AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1265460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265460 MtrunA17_Chr4g0072291 95.174 518 15 3 1 514 1 512 0.0 1000
Msa1265460 MtrunA17_Chr2g0328971 80.465 215 41 1 148 361 156 370 5.22e-125 375
Msa1265460 MtrunA17_Chr4g0032631 81.395 215 36 2 149 359 153 367 5.55e-124 373
Msa1265460 MtrunA17_Chr7g0249621 88.325 197 19 2 149 341 249 445 7.18e-124 378
Msa1265460 MtrunA17_Chr8g0343851 80.095 211 41 1 142 351 231 441 4.97e-121 367
Msa1265460 MtrunA17_Chr1g0152161 63.296 267 86 2 91 357 256 510 7.76e-116 356
Msa1265460 MtrunA17_Chr3g0135161 62.547 267 85 3 91 357 254 505 1.93e-113 350
Msa1265460 MtrunA17_Chr4g0054351 72.936 218 56 1 143 357 294 511 2.66e-112 347
Msa1265460 MtrunA17_Chr5g0412151 84.264 197 28 1 148 341 213 409 2.72e-111 340
Msa1265460 MtrunA17_Chr5g0400871 73.892 203 50 1 155 354 245 447 1.69e-107 331
Msa1265460 MtrunA17_Chr4g0073521 72.892 166 45 0 158 323 46 211 9.23e-89 276
Msa1265460 MtrunA17_Chr7g0216601 63.298 188 66 1 139 323 45 232 3.17e-80 255
Msa1265460 MtrunA17_Chr8g0367311 59.048 210 76 4 196 402 22 224 1.97e-79 248
Msa1265460 MtrunA17_Chr6g0452911 52.632 247 77 5 163 399 65 281 8.96e-79 251
Msa1265460 MtrunA17_Chr8g0355191 68.072 166 53 0 158 323 54 219 2.74e-78 251
Msa1265460 MtrunA17_Chr4g0002011 70.414 169 47 1 158 323 35 203 9.87e-77 244
Msa1265460 MtrunA17_Chr8g0355181 66.867 166 55 0 158 323 55 220 4.72e-73 236
Msa1265460 MtrunA17_Chr8g0362541 62.245 196 60 1 142 323 52 247 1.33e-72 236
Msa1265460 MtrunA17_Chr2g0305821 67.857 112 36 0 158 269 46 157 4.48e-44 153
Msa1265460 MtrunA17_Chr7g0257361 61.538 130 42 2 157 283 55 179 4.42e-42 155
Msa1265460 MtrunA17_Chr4g0053181 49.239 197 84 5 163 356 177 360 1.58e-40 153
Msa1265460 MtrunA17_Chr7g0263581 39.427 279 141 8 134 392 110 380 3.11e-40 151
Msa1265460 MtrunA17_Chr5g0401891 53.333 165 63 4 163 324 153 306 1.08e-39 150
Msa1265460 MtrunA17_Chr4g0030191 49.091 165 70 4 163 324 132 285 3.92e-37 142
Msa1265460 MtrunA17_Chr2g0325911 45.556 180 75 4 145 324 117 273 3.87e-32 128
Msa1265460 MtrunA17_Chr2g0305831 76.271 59 14 0 265 323 1 59 5.85e-26 105
Msa1265460 MtrunA17_Chr3g0126151 45.263 95 51 1 207 301 47 140 4.02e-22 92.4
Msa1265460 MtrunA17_Chr8g0367321 78.000 50 11 0 148 197 144 193 1.16e-21 93.2
Msa1265460 MtrunA17_Chr1g0170761 36.691 139 74 4 143 278 118 245 2.97e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265460 AT5G57390.1 54.004 487 158 14 75 514 91 558 1.02e-147 435
Msa1265460 AT5G57390.2 57.477 428 137 11 118 514 27 440 2.55e-145 424
Msa1265460 AT1G51190.1 80.734 218 38 2 146 359 174 391 1.10e-124 376
Msa1265460 AT3G20840.1 81.043 211 36 2 148 354 167 377 4.21e-122 370
Msa1265460 AT5G17430.2 86.154 195 23 2 146 336 132 326 7.79e-121 365
Msa1265460 AT5G17430.1 86.154 195 23 2 146 336 194 388 4.93e-120 365
Msa1265460 AT1G72570.1 83.158 190 29 1 149 335 210 399 8.75e-116 348
Msa1265460 AT4G37750.1 64.130 276 83 6 150 412 271 543 7.77e-114 348
Msa1265460 AT5G10510.2 72.803 239 52 3 121 359 200 425 5.83e-110 337
Msa1265460 AT5G65510.4 73.568 227 46 2 121 344 143 358 1.51e-108 330
Msa1265460 AT5G65510.2 73.568 227 46 2 121 344 201 416 3.34e-108 332
Msa1265460 AT5G65510.1 73.568 227 46 2 121 344 201 416 3.34e-108 332
Msa1265460 AT5G10510.7 71.901 242 52 4 121 359 200 428 7.19e-108 332
Msa1265460 AT5G10510.5 71.901 242 52 4 121 359 200 428 7.19e-108 332
Msa1265460 AT5G10510.1 71.901 242 52 4 121 359 223 451 1.32e-107 332
Msa1265460 AT5G10510.6 71.901 242 52 4 121 359 238 466 1.67e-107 332
Msa1265460 AT5G10510.4 71.901 242 52 4 121 359 238 466 1.67e-107 332
Msa1265460 AT5G10510.3 70.417 240 46 3 143 359 249 486 4.71e-103 322
Msa1265460 AT5G65510.3 74.857 175 30 2 121 292 143 306 7.89e-91 280
Msa1265460 AT3G54320.3 67.456 169 52 1 158 323 61 229 2.18e-70 231
Msa1265460 AT3G54320.1 67.456 169 52 1 158 323 61 229 3.47e-70 231
Msa1265460 AT1G16060.1 69.231 169 49 1 158 323 54 222 2.17e-65 216
Msa1265460 AT1G79700.2 68.639 169 50 1 158 323 48 216 5.34e-65 213
Msa1265460 AT1G79700.1 64.497 169 47 2 158 323 48 206 5.02e-57 192
Msa1265460 AT3G54320.4 68.750 128 40 0 196 323 36 163 2.05e-51 179
Msa1265460 AT3G54320.5 68.750 128 40 0 196 323 36 163 2.05e-51 179
Msa1265460 AT1G79700.4 69.531 128 39 0 196 323 7 134 9.31e-46 160
Msa1265460 AT1G79700.3 69.531 128 39 0 196 323 13 140 1.06e-45 160
Msa1265460 AT1G16060.3 68.657 134 42 0 190 323 19 152 2.59e-45 160
Msa1265460 AT2G41710.1 59.848 132 45 2 155 283 63 189 2.08e-41 154
Msa1265460 AT2G41710.4 59.848 132 45 2 155 283 63 189 3.48e-41 154
Msa1265460 AT4G36920.1 50.259 193 81 5 143 332 113 293 7.11e-41 152
Msa1265460 AT4G36920.2 50.259 193 81 5 143 332 113 293 7.11e-41 152
Msa1265460 AT2G41710.2 57.664 137 45 3 155 283 63 194 5.38e-39 147
Msa1265460 AT2G28550.3 39.163 263 123 7 163 407 154 397 3.20e-38 146
Msa1265460 AT5G67180.2 47.150 193 85 5 135 324 72 250 9.17e-38 140
Msa1265460 AT5G67180.1 40.840 262 126 7 135 384 72 316 1.24e-37 142
Msa1265460 AT5G67180.3 46.842 190 90 4 135 324 72 250 1.54e-37 139
Msa1265460 AT2G41710.3 47.305 167 45 3 155 283 63 224 9.29e-35 136
Msa1265460 AT1G16060.2 72.727 88 24 0 236 323 1 88 5.29e-33 125
Msa1265460 AT1G16060.4 72.727 88 24 0 236 323 1 88 5.29e-33 125
Msa1265460 AT1G16060.6 72.727 88 24 0 236 323 1 88 5.29e-33 125
Msa1265460 AT1G16060.5 72.727 88 24 0 236 323 1 88 5.29e-33 125
Msa1265460 AT3G54320.2 71.591 88 25 0 236 323 1 88 4.91e-32 125
Msa1265460 AT2G28550.4 48.571 140 58 4 163 299 154 282 5.94e-30 120
Msa1265460 AT2G28550.1 36.502 263 115 8 163 407 154 382 8.09e-30 122
Msa1265460 AT5G67180.4 44.118 170 84 4 135 304 72 230 2.35e-28 114
Msa1265460 AT2G28550.2 48.413 126 51 4 163 285 154 268 3.57e-25 105
Msa1265460 AT2G28550.6 48.413 126 51 4 163 285 154 268 3.57e-25 105
Msa1265460 AT2G28550.5 35.242 227 108 5 196 407 14 216 1.08e-22 98.6
Msa1265460 AT2G39250.2 42.857 112 51 3 163 271 109 210 1.45e-22 97.1
Msa1265460 AT2G39250.1 42.857 112 51 3 163 271 109 210 4.06e-22 97.8
Msa1265460 AT2G39250.1 40.196 102 55 3 235 332 80 179 9.86e-11 63.5
Msa1265460 AT5G60120.2 30.220 364 183 17 139 480 141 455 1.74e-19 92.0
Msa1265460 AT5G60120.6 37.569 181 80 7 139 316 141 291 5.48e-19 90.1
Msa1265460 AT5G60120.1 37.569 181 80 7 139 316 141 291 5.82e-19 90.1
Msa1265460 AT5G60120.4 42.105 152 66 6 138 284 140 274 6.67e-19 89.4
Msa1265460 AT5G60120.3 42.105 152 66 6 138 284 140 274 6.67e-19 89.4
Msa1265460 AT5G60120.5 42.105 152 66 6 138 284 140 274 6.67e-19 89.4
Msa1265460 AT1G16060.7 79.310 58 12 0 266 323 9 66 6.84e-19 85.1
Msa1265460 AT3G54990.2 47.170 106 43 3 163 265 123 218 2.06e-17 82.4
Msa1265460 AT3G54990.1 47.170 106 43 3 163 265 123 218 7.04e-17 82.4
Msa1265460 AT1G03800.1 35.338 133 75 3 231 363 22 143 3.62e-11 63.9
Msa1265460 AT1G36060.1 38.318 107 60 2 252 358 133 233 3.87e-11 64.7
Msa1265460 AT2G20880.1 44.578 83 45 1 236 318 161 242 8.09e-11 63.9

Find 156 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTTCAAACGCCAATATT+TGG 0.167429 8_2:+69821696 Msa1265460:CDS
TTGAATTGGTTCGTTGATTT+TGG 0.203511 8_2:-69821241 None:intergenic
AGGATTAGGAAAGTTATTAT+TGG 0.265279 8_2:-69818384 None:intergenic
AAGACCCTTCTACCGATTAT+TGG 0.267990 8_2:+69821334 Msa1265460:CDS
TGAAGCTTAGTTGGTTTCTT+TGG 0.274376 8_2:-69818245 None:intergenic
CAGCACTGCTAGGGTTATTA+TGG 0.274613 8_2:-69821374 None:intergenic
GTAGAAGGGTCTTGTTTAAT+AGG 0.278427 8_2:-69821324 None:intergenic
GCTCATTCCATTCCAAATAT+TGG 0.285129 8_2:-69821708 None:intergenic
ATCTTGCTGCTCTCAAGTAC+TGG 0.314872 8_2:+69820031 Msa1265460:CDS
CTTATTTGATAAACCTCCTA+TGG 0.315963 8_2:-69821184 None:intergenic
AAGGTAGAGGTCTTTGTTTC+CGG 0.318164 8_2:-69820630 None:intergenic
GGAGATATGAAGCACATCTA+TGG 0.326305 8_2:+69819132 Msa1265460:CDS
CTTGTGGCTGCCGGTGAATA+TGG 0.331498 8_2:-69818657 None:intergenic
AACGACCACTACCAACTTTC+CGG 0.338959 8_2:+69820059 Msa1265460:CDS
TTACTATTTAAAGAAAATAA+TGG 0.350585 8_2:-69821588 None:intergenic
CACAAGTTCGAGGACTTCTT+AGG 0.357176 8_2:+69818674 Msa1265460:CDS
ATTGCAGCAATCAAATTTAG+AGG 0.358044 8_2:+69821093 Msa1265460:CDS
GAGGTGTTGTTAGTGTTTGT+AGG 0.362481 8_2:-69818344 None:intergenic
GAAGATGAGTGGGAAGGATT+AGG 0.362867 8_2:-69818398 None:intergenic
GACATGATTTATGAGTATAA+TGG 0.370754 8_2:+69822048 Msa1265460:three_prime_UTR
TTGGACCAATAATCGGTAGA+AGG 0.379956 8_2:-69821339 None:intergenic
TATGAGAATGGTACTAGTTA+TGG 0.381199 8_2:+69821621 Msa1265460:CDS
GGTATTATAGAGGAAGGATA+AGG 0.383296 8_2:-69818452 None:intergenic
ATTATGGAAGCCGAGAATAT+TGG 0.385997 8_2:-69821358 None:intergenic
ACATGATTTATGAGTATAAT+GGG 0.387504 8_2:+69822049 Msa1265460:three_prime_UTR
CCTGGTGACACCGCGATAGA+TGG 0.390623 8_2:-69818903 None:intergenic
CGATGGCGTTGAAGCTTAGT+TGG 0.395432 8_2:-69818254 None:intergenic
GACTTGGCTTTGCATGTGTT+TGG 0.396154 8_2:-69821674 None:intergenic
GGAATGAGCTCATGCATATC+AGG 0.398293 8_2:+69821722 Msa1265460:exon
AGGTGTTGTTAGTGTTTGTA+GGG 0.402140 8_2:-69818343 None:intergenic
TCTATTTCGTCAGATTGAAT+TGG 0.402860 8_2:-69821255 None:intergenic
ATCAATCTCCTTCTCATAGT+TGG 0.408692 8_2:-69820206 None:intergenic
GTATTATAGAGGAAGGATAA+GGG 0.409045 8_2:-69818451 None:intergenic
AATGGTACTAGTTATGGAAA+TGG 0.413030 8_2:+69821627 Msa1265460:CDS
ATGGTACCAACACAATGAAA+TGG 0.414251 8_2:-69822091 None:intergenic
GATTATTGGTCCAATATTCT+CGG 0.416679 8_2:+69821348 Msa1265460:CDS
GTTATGGAAATGGAAATAAC+TGG 0.419371 8_2:+69821637 Msa1265460:CDS
ACAATAGCTGCTTGCTTCCC+TGG 0.422931 8_2:+69818800 Msa1265460:CDS
TGTAATCCATTTCATTGTGT+TGG 0.424726 8_2:+69822085 Msa1265460:three_prime_UTR
TTGCCTGCCATCGGCCATGC+TGG 0.430696 8_2:-69820592 None:intergenic
GGTTCGGTGGGGTAGCCACC+AGG 0.431604 8_2:-69818818 None:intergenic
GAGAATATTGGACCAATAAT+CGG 0.435797 8_2:-69821346 None:intergenic
TTATCACTGTTGGTATTTGA+AGG 0.440953 8_2:-69821438 None:intergenic
TGACGACGAAGATGAAGAAT+TGG 0.441913 8_2:-69821535 None:intergenic
GTTGCGGCGGCTGTTCTTGT+TGG 0.443667 8_2:-69818710 None:intergenic
AGGTTCGGAGTTTGGTTCGG+TGG 0.444923 8_2:-69818831 None:intergenic
GAGAGATGGTGGTATTATAG+AGG 0.451776 8_2:-69818462 None:intergenic
GCAAGGATAGGCAGAGTTGC+CGG 0.451955 8_2:+69820611 Msa1265460:CDS
TAATTTAGGTGGCTATGATA+AGG 0.452771 8_2:+69819984 Msa1265460:intron
GGTTCGGAGTTTGGTTCGGT+GGG 0.458866 8_2:-69818830 None:intergenic
TTGAGGTTCGGAGTTTGGTT+CGG 0.459991 8_2:-69818834 None:intergenic
TCTTGCTGCTCTCAAGTACT+GGG 0.460585 8_2:+69820032 Msa1265460:CDS
GGCTGTTCTTGTTGGTGCAA+CGG 0.461915 8_2:-69818702 None:intergenic
AAGGATAAGGGGTGAAGGAG+TGG 0.462972 8_2:-69818439 None:intergenic
GATGGTGGTATTATAGAGGA+AGG 0.464335 8_2:-69818458 None:intergenic
GAAAATAATGGCGTTGCGAA+TGG 0.470205 8_2:-69821576 None:intergenic
CCATCTATCGCGGTGTCACC+AGG 0.475525 8_2:+69818903 Msa1265460:CDS
GTTCCTCCATCTGTTGCCTC+TGG 0.480278 8_2:-69818623 None:intergenic
AGGGTCTTGTTTAATAGGTA+AGG 0.484892 8_2:-69821319 None:intergenic
GATGATGATGAAAGTGTTGA+AGG 0.487520 8_2:+69821798 Msa1265460:three_prime_UTR
GAGGTGAGAGGAAGATGAGT+GGG 0.487978 8_2:-69818408 None:intergenic
AGAGGAAGGATAAGGGGTGA+AGG 0.490294 8_2:-69818444 None:intergenic
ACAATGAAATGGATTACAAA+AGG 0.493633 8_2:-69822080 None:intergenic
GTCCTCGAACTTGTGGCTGC+CGG 0.496373 8_2:-69818666 None:intergenic
TCAAACGCCAATATTTGGAA+TGG 0.498126 8_2:+69821701 Msa1265460:CDS
AATAGTAGTAGTTATGAGAA+TGG 0.502038 8_2:+69821609 Msa1265460:CDS
TATTGGAAAGAGAGAAAGCG+AGG 0.505616 8_2:-69818367 None:intergenic
TCATCCATCTTCAACTTCGT+TGG 0.509118 8_2:+69821278 Msa1265460:CDS
GGTGAATATGGAGAGGTTTG+TGG 0.510555 8_2:-69818645 None:intergenic
TGCCTATCCTTGCCTGCCAT+CGG 0.511733 8_2:-69820601 None:intergenic
GTGGTAGTAACAGCACTGCT+AGG 0.512894 8_2:-69821384 None:intergenic
GGAAAGAGAGAAAGCGAGGG+AGG 0.518672 8_2:-69818363 None:intergenic
TGAAGGAGTGGAAAAGAGAG+AGG 0.524119 8_2:-69818427 None:intergenic
AGCATGGCCGATGGCAGGCA+AGG 0.524298 8_2:+69820594 Msa1265460:CDS
TGCCTCTGGTGAAGCTTCCA+CGG 0.528484 8_2:-69818609 None:intergenic
AATAGTTCTCTTCCCATAGG+AGG 0.530212 8_2:+69821171 Msa1265460:CDS
GGCCGATGGCAGGCAAGGAT+AGG 0.531558 8_2:+69820599 Msa1265460:CDS
GAAAACTGCTGAAGCTGTGT+GGG 0.532869 8_2:-69818740 None:intergenic
CCACCAGCATGGCCGATGGC+AGG 0.533190 8_2:+69820589 Msa1265460:CDS
TACTGAAAGTGCCAAGGTAG+AGG 0.534747 8_2:-69820643 None:intergenic
CTAAGAAGTCCTCGAACTTG+TGG 0.537216 8_2:-69818673 None:intergenic
CCTGCCATCGGCCATGCTGG+TGG 0.544113 8_2:-69820589 None:intergenic
GGAATTATATATATCATTGT+CGG 0.547335 8_2:-69818765 None:intergenic
GTGGAAAAGAGAGAGGTGAG+AGG 0.548112 8_2:-69818420 None:intergenic
ATTGGAAAGAGAGAAAGCGA+GGG 0.549140 8_2:-69818366 None:intergenic
GTTGCCAACGAAGTTGAAGA+TGG 0.549484 8_2:-69821282 None:intergenic
AGAGGTGAGAGGAAGATGAG+TGG 0.549550 8_2:-69818409 None:intergenic
GCAAATAGTTCTCTTCCCAT+AGG 0.555242 8_2:+69821168 Msa1265460:CDS
CCACCATCTCTCACCCTCAC+AGG 0.557858 8_2:+69818473 Msa1265460:CDS
TGAGAGGAAGATGAGTGGGA+AGG 0.558701 8_2:-69818404 None:intergenic
GAGATATGAAGCACATCTAT+GGG 0.559005 8_2:+69819133 Msa1265460:CDS
CAAAATATGAACGGAAGCAG+TGG 0.559047 8_2:+69821510 Msa1265460:CDS
AGGGAAGGACAAAGTAGGAA+AGG 0.559779 8_2:+69819170 Msa1265460:CDS
GTACAAACTTACCGGAAAGT+TGG 0.561227 8_2:-69820070 None:intergenic
CAATTTACAGAGGGGTAACA+AGG 0.562932 8_2:+69820420 Msa1265460:CDS
TTATTTGATAAACCTCCTAT+GGG 0.563688 8_2:-69821183 None:intergenic
TGGTAGTAACAGCACTGCTA+GGG 0.567073 8_2:-69821383 None:intergenic
CACCGGCAGCCACAAGTTCG+AGG 0.568522 8_2:+69818664 Msa1265460:CDS
GGAAAGTTGGTAGTGGTCGT+TGG 0.570366 8_2:-69820057 None:intergenic
GGAAACAAAGACCTCTACCT+TGG 0.572572 8_2:+69820632 Msa1265460:CDS
GTGTTGTTAGTGTTTGTAGG+GGG 0.575757 8_2:-69818341 None:intergenic
AAGAGTAGTGGTTTCTCAAG+AGG 0.576512 8_2:+69820392 Msa1265460:CDS
GATGGAGGTGCGTTGTCCGA+AGG 0.579181 8_2:-69818885 None:intergenic
GCTTCACCAGAGGCAACAGA+TGG 0.579683 8_2:+69818617 Msa1265460:CDS
TACTACCACACCATTCAACA+TGG 0.582642 8_2:+69821398 Msa1265460:CDS
TTTCAGCAACAAAATATGAA+CGG 0.583240 8_2:+69821501 Msa1265460:CDS
TCAGATTTCCAACTATGAGA+AGG 0.583626 8_2:+69820198 Msa1265460:intron
TATGGGACAATAGTTGCAGA+AGG 0.584131 8_2:+69819150 Msa1265460:CDS
GACACCACCAGCATGGCCGA+TGG 0.584738 8_2:+69820585 Msa1265460:intron
GACAATAGTTGCAGAAGGGA+AGG 0.585408 8_2:+69819155 Msa1265460:CDS
TATTATAGAGGAAGGATAAG+GGG 0.586147 8_2:-69818450 None:intergenic
TCATGCATATCAGGTGCTGA+TGG 0.589848 8_2:+69821731 Msa1265460:three_prime_UTR
GGAGAGAGAAAGACCTGTGA+GGG 0.590470 8_2:-69818486 None:intergenic
TGGACCAATAATCGGTAGAA+GGG 0.591088 8_2:-69821338 None:intergenic
ATATGAACGTGAAATATCGA+TGG 0.594272 8_2:-69818271 None:intergenic
CAAAGTAGGAAAGGAAGACA+AGG 0.595973 8_2:+69819179 Msa1265460:CDS
GAAGGACAGTGTTGTAGTGA+TGG 0.596070 8_2:+69821820 Msa1265460:three_prime_UTR
GCAGAAGGGAAGGACAAAGT+AGG 0.597553 8_2:+69819165 Msa1265460:CDS
GGTGTTGTTAGTGTTTGTAG+GGG 0.599101 8_2:-69818342 None:intergenic
GTTCGGAGTTTGGTTCGGTG+GGG 0.599227 8_2:-69818829 None:intergenic
GGTTTCTGATGCAGAATCTG+TGG 0.599958 8_2:-69821220 None:intergenic
ATGATGAAAGTGTTGAAGGA+AGG 0.600265 8_2:+69821802 Msa1265460:three_prime_UTR
TCATGTTATAGGAAGAGTAG+TGG 0.600719 8_2:+69820380 Msa1265460:intron
ATTGGCGTTTGAAACAGACT+TGG 0.602086 8_2:-69821690 None:intergenic
GTTCGGTGGGGTAGCCACCA+GGG 0.604192 8_2:-69818817 None:intergenic
ATTATATATATCATTGTCGG+TGG 0.604777 8_2:-69818762 None:intergenic
CTGTGTGGGTGCACACGTTG+CGG 0.605215 8_2:-69818726 None:intergenic
GAGGAGCATCAATTTACAGA+GGG 0.606917 8_2:+69820411 Msa1265460:CDS
ATTGTGGGGATTATCACTGT+TGG 0.607060 8_2:-69821448 None:intergenic
AATTTGTTGCTTCTCTACGA+AGG 0.609124 8_2:+69820254 Msa1265460:CDS
ACAAACCTCTCCATATTCAC+CGG 0.611418 8_2:+69818647 Msa1265460:CDS
TCACCAGAGGCAACAGATGG+AGG 0.617655 8_2:+69818620 Msa1265460:CDS
TGCAAAGCAATAGAATCAGA+AGG 0.621670 8_2:+69821860 Msa1265460:three_prime_UTR
AATAGAATCAGAAGGAAACA+AGG 0.627213 8_2:+69821868 Msa1265460:three_prime_UTR
TCCAGGAAGCAGCAACGCCG+TGG 0.632938 8_2:+69818592 Msa1265460:intron
CGCCGTGGAAGCTTCACCAG+AGG 0.633581 8_2:+69818607 Msa1265460:CDS
CCTGTGAGGGTGAGAGATGG+TGG 0.636945 8_2:-69818473 None:intergenic
GGAAAACTGCTGAAGCTGTG+TGG 0.638121 8_2:-69818741 None:intergenic
TGTGGGTGCACACGTTGCGG+CGG 0.646698 8_2:-69818723 None:intergenic
TGGTGCAGACACCACCAGCA+TGG 0.647953 8_2:+69820578 Msa1265460:intron
AAAATCCATGTTGAATGGTG+TGG 0.648019 8_2:-69821403 None:intergenic
ACTTACCGGAAAGTTGGTAG+TGG 0.649056 8_2:-69820064 None:intergenic
ATGGGACAATAGTTGCAGAA+GGG 0.651127 8_2:+69819151 Msa1265460:CDS
GCGTTGTCCGAAGGTATCAA+CGG 0.652510 8_2:-69818876 None:intergenic
AGTACATACTGAAAGTGCCA+AGG 0.656101 8_2:-69820649 None:intergenic
GGTGACACCGCGATAGATGG+AGG 0.661765 8_2:-69818900 None:intergenic
AGACCTGTGAGGGTGAGAGA+TGG 0.662144 8_2:-69818476 None:intergenic
AAGTGAATAATACACAACAA+TGG 0.674679 8_2:-69822110 None:intergenic
ATAGCTGCTTGCTTCCCTGG+TGG 0.677407 8_2:+69818803 Msa1265460:CDS
CTTGCTGCTCTCAAGTACTG+GGG 0.677546 8_2:+69820033 Msa1265460:CDS
GGCTGCCGGTGAATATGGAG+AGG 0.695158 8_2:-69818652 None:intergenic
CAACGCACCTCCATCTATCG+CGG 0.695636 8_2:+69818893 Msa1265460:CDS
AGAGGAGCATCAATTTACAG+AGG 0.704191 8_2:+69820410 Msa1265460:CDS
GGGAGAGAGAAAGACCTGTG+AGG 0.704212 8_2:-69818487 None:intergenic
AAATTGAAGACTTAAAACAA+AGG 0.710279 8_2:-69821967 None:intergenic
AGGAGCATCAATTTACAGAG+GGG 0.728920 8_2:+69820412 Msa1265460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATAAATTTTTAAAAAAA+TGG - chr8_2:69820878-69820897 None:intergenic 0.0%
!!! TTTGTTTTTTGTTATAATTT+AGG + chr8_2:69819970-69819989 Msa1265460:intron 10.0%
!!! AAATTATATGAATAGTTTTA+TGG + chr8_2:69820341-69820360 Msa1265460:intron 10.0%
!! TTATTTATAGTATACTATAA+AGG + chr8_2:69820777-69820796 Msa1265460:intron 10.0%
!! TATTTATAGTATACTATAAA+GGG + chr8_2:69820778-69820797 Msa1265460:intron 10.0%
!! TATATTATTATTATGTCATT+AGG - chr8_2:69820935-69820954 None:intergenic 10.0%
!! TAAATTGAAAAAAAATGATT+AGG - chr8_2:69821027-69821046 None:intergenic 10.0%
!! TTACTATTTAAAGAAAATAA+TGG - chr8_2:69821591-69821610 None:intergenic 10.0%
!! TGAAAGATTATTTATATGTA+GGG + chr8_2:69819222-69819241 Msa1265460:intron 15.0%
!! TAGTAGTATAAATTTGAATA+AGG + chr8_2:69819299-69819318 Msa1265460:intron 15.0%
!! TTATTATGATCAAAATAGAT+CGG + chr8_2:69819649-69819668 Msa1265460:intron 15.0%
!! CATTAAACAGTAAAATAAAA+GGG - chr8_2:69820114-69820133 None:intergenic 15.0%
!!! AATGTTTTACATTTTTTTGT+TGG + chr8_2:69820166-69820185 Msa1265460:intron 15.0%
!! AAACAGTTTAATAAAAAAAC+TGG - chr8_2:69820825-69820844 None:intergenic 15.0%
!! AAAATTGAAAAACACAAAAA+AGG - chr8_2:69821995-69822014 None:intergenic 15.0%
!!! TTTTTGTGTTTTTCAATTTT+TGG + chr8_2:69821995-69822014 Msa1265460:three_prime_UTR 15.0%
!!! ACTTTTTTTTTTTCATTTCC+AGG + chr8_2:69818575-69818594 Msa1265460:intron 20.0%
!! GGAATTATATATATCATTGT+CGG - chr8_2:69818768-69818787 None:intergenic 20.0%
!! GTGAAAGATTATTTATATGT+AGG + chr8_2:69819221-69819240 Msa1265460:intron 20.0%
!! TATTATGATCAAAATAGATC+GGG + chr8_2:69819650-69819669 Msa1265460:intron 20.0%
!!! TAATCATTTAGCTCATAAAA+TGG + chr8_2:69819943-69819962 Msa1265460:intron 20.0%
!!! GTTTTTTGTTATAATTTAGG+TGG + chr8_2:69819973-69819992 Msa1265460:intron 20.0%
!! GCATTAAACAGTAAAATAAA+AGG - chr8_2:69820115-69820134 None:intergenic 20.0%
!!! TTTATTTTCCTTCATGTTAT+AGG + chr8_2:69820369-69820388 Msa1265460:intron 20.0%
!!! TGATAAATTCATATTCGTTT+TGG + chr8_2:69820477-69820496 Msa1265460:intron 20.0%
!!! TTGAAACAACTTTAATTTGT+TGG + chr8_2:69820558-69820577 Msa1265460:intron 20.0%
!! TAAAAAAATATTAGATGGGT+CGG - chr8_2:69820760-69820779 None:intergenic 20.0%
!! AATAAAAAAACTGGCAAAAA+TGG - chr8_2:69820816-69820835 None:intergenic 20.0%
!! AAATTGAAGACTTAAAACAA+AGG - chr8_2:69821970-69821989 None:intergenic 20.0%
!! ACATGATTTATGAGTATAAT+GGG + chr8_2:69822049-69822068 Msa1265460:three_prime_UTR 20.0%
!!! CCATAGTTTGATTTTGTTTT+TGG - chr8_2:69818304-69818323 None:intergenic 25.0%
! CCAAAAACAAAATCAAACTA+TGG + chr8_2:69818301-69818320 Msa1265460:exon 25.0%
! AGGATTAGGAAAGTTATTAT+TGG - chr8_2:69818387-69818406 None:intergenic 25.0%
! ATTATATATATCATTGTCGG+TGG - chr8_2:69818765-69818784 None:intergenic 25.0%
! AAAAAGCTAGCCAAAATATA+TGG + chr8_2:69818973-69818992 Msa1265460:intron 25.0%
!! TTGTCTATGTCCATATATTT+TGG - chr8_2:69818986-69819005 None:intergenic 25.0%
!! TGATAATTTTCAGACATAGA+TGG + chr8_2:69819105-69819124 Msa1265460:intron 25.0%
! ATTATTTATATGTAGGGACT+AGG + chr8_2:69819228-69819247 Msa1265460:intron 25.0%
! TTATTTATATGTAGGGACTA+GGG + chr8_2:69819229-69819248 Msa1265460:intron 25.0%
!! ATCTTTTGCTTCAGTTTATT+TGG + chr8_2:69819756-69819775 Msa1265460:intron 25.0%
!! TCTTTTGCTTCAGTTTATTT+GGG + chr8_2:69819757-69819776 Msa1265460:intron 25.0%
!!! ACCAAATCATCATTTTGTTT+TGG + chr8_2:69819836-69819855 Msa1265460:intron 25.0%
! GATTATTTGTACAAACTTAC+CGG - chr8_2:69820081-69820100 None:intergenic 25.0%
! ACATTTATGAAAACCGTTAA+AGG - chr8_2:69820151-69820170 None:intergenic 25.0%
!! AAGTTAAAACTCAAAGGTAT+TGG - chr8_2:69820448-69820467 None:intergenic 25.0%
! CACAAAAAGTTAAAACTCAA+AGG - chr8_2:69820454-69820473 None:intergenic 25.0%
!!! CTTTGAGTTTTAACTTTTTG+TGG + chr8_2:69820452-69820471 Msa1265460:intron 25.0%
! AATCTAATGATATACACTAG+GGG - chr8_2:69820503-69820522 None:intergenic 25.0%
! GAATCTAATGATATACACTA+GGG - chr8_2:69820504-69820523 None:intergenic 25.0%
! AGAATCTAATGATATACACT+AGG - chr8_2:69820505-69820524 None:intergenic 25.0%
! TGATGTCAATATATGGATAA+TGG - chr8_2:69820678-69820697 None:intergenic 25.0%
! TGTATAGTGATGTCAATATA+TGG - chr8_2:69820685-69820704 None:intergenic 25.0%
! TTATTTGATAAACCTCCTAT+GGG - chr8_2:69821186-69821205 None:intergenic 25.0%
! TTTCAGCAACAAAATATGAA+CGG + chr8_2:69821501-69821520 Msa1265460:CDS 25.0%
! AATAGTAGTAGTTATGAGAA+TGG + chr8_2:69821609-69821628 Msa1265460:CDS 25.0%
! GACATGATTTATGAGTATAA+TGG + chr8_2:69822048-69822067 Msa1265460:three_prime_UTR 25.0%
!!! ACAATGAAATGGATTACAAA+AGG - chr8_2:69822083-69822102 None:intergenic 25.0%
! AAGTGAATAATACACAACAA+TGG - chr8_2:69822113-69822132 None:intergenic 25.0%
ATATGAACGTGAAATATCGA+TGG - chr8_2:69818274-69818293 None:intergenic 30.0%
TATTATAGAGGAAGGATAAG+GGG - chr8_2:69818453-69818472 None:intergenic 30.0%
GTATTATAGAGGAAGGATAA+GGG - chr8_2:69818454-69818473 None:intergenic 30.0%
TTAGAGAGAAACTGAGATAT+TGG - chr8_2:69818534-69818553 None:intergenic 30.0%
!!! AAGGTATCAACGGTTTTTTT+AGG - chr8_2:69818869-69818888 None:intergenic 30.0%
AAAAAAACCGTTGATACCTT+CGG + chr8_2:69818869-69818888 Msa1265460:CDS 30.0%
! AATTTTCAGACATAGATGGA+CGG + chr8_2:69819109-69819128 Msa1265460:intron 30.0%
TCTTTAACTCCCATTTATTC+CGG - chr8_2:69819423-69819442 None:intergenic 30.0%
TTCAAAACCATTCTCAATCA+AGG + chr8_2:69819470-69819489 Msa1265460:intron 30.0%
CAGGTACAAAAGCAAAAAAA+AGG - chr8_2:69819708-69819727 None:intergenic 30.0%
!!! CTTTTTTTTGCTTTTGTACC+TGG + chr8_2:69819706-69819725 Msa1265460:intron 30.0%
GCCAAAACAAAATGATGATT+TGG - chr8_2:69819840-69819859 None:intergenic 30.0%
CATTAAAATGTACATGAAGC+AGG - chr8_2:69819870-69819889 None:intergenic 30.0%
TAATTTAGGTGGCTATGATA+AGG + chr8_2:69819984-69820003 Msa1265460:intron 30.0%
ATTGCAGCAATCAAATTTAG+AGG + chr8_2:69821093-69821112 Msa1265460:CDS 30.0%
CTTATTTGATAAACCTCCTA+TGG - chr8_2:69821187-69821206 None:intergenic 30.0%
!!! TTGAATTGGTTCGTTGATTT+TGG - chr8_2:69821244-69821263 None:intergenic 30.0%
TCTATTTCGTCAGATTGAAT+TGG - chr8_2:69821258-69821277 None:intergenic 30.0%
GAGAATATTGGACCAATAAT+CGG - chr8_2:69821349-69821368 None:intergenic 30.0%
GATTATTGGTCCAATATTCT+CGG + chr8_2:69821348-69821367 Msa1265460:CDS 30.0%
TTATCACTGTTGGTATTTGA+AGG - chr8_2:69821441-69821460 None:intergenic 30.0%
TATGAGAATGGTACTAGTTA+TGG + chr8_2:69821621-69821640 Msa1265460:CDS 30.0%
AATGGTACTAGTTATGGAAA+TGG + chr8_2:69821627-69821646 Msa1265460:CDS 30.0%
GTTATGGAAATGGAAATAAC+TGG + chr8_2:69821637-69821656 Msa1265460:CDS 30.0%
AATAGAATCAGAAGGAAACA+AGG + chr8_2:69821868-69821887 Msa1265460:three_prime_UTR 30.0%
TGTAATCCATTTCATTGTGT+TGG + chr8_2:69822085-69822104 Msa1265460:three_prime_UTR 30.0%
! TGAAGCTTAGTTGGTTTCTT+TGG - chr8_2:69818248-69818267 None:intergenic 35.0%
!! AGGTGTTGTTAGTGTTTGTA+GGG - chr8_2:69818346-69818365 None:intergenic 35.0%
GGTATTATAGAGGAAGGATA+AGG - chr8_2:69818455-69818474 None:intergenic 35.0%
! AGCTATTGTTTTCTTCAGCT+CGG - chr8_2:69818789-69818808 None:intergenic 35.0%
!! TTTTTAGGAGAAGGCTTTTG+AGG - chr8_2:69818854-69818873 None:intergenic 35.0%
!!! TCAACGGTTTTTTTAGGAGA+AGG - chr8_2:69818863-69818882 None:intergenic 35.0%
AAACATGCACTAAACATACC+TGG - chr8_2:69818924-69818943 None:intergenic 35.0%
! ATTTTCAGACATAGATGGAC+GGG + chr8_2:69819110-69819129 Msa1265460:intron 35.0%
GAGATATGAAGCACATCTAT+GGG + chr8_2:69819133-69819152 Msa1265460:CDS 35.0%
TCTGAATCCTTGATTGAGAA+TGG - chr8_2:69819480-69819499 None:intergenic 35.0%
! TGAACTTTGAAGATTCTGAC+CGG + chr8_2:69819681-69819700 Msa1265460:intron 35.0%
ACAAAAGCAAAAAAAAGGCC+CGG - chr8_2:69819703-69819722 None:intergenic 35.0%
AACTATTCACGCTCCTTTAA+CGG + chr8_2:69820135-69820154 Msa1265460:intron 35.0%
TCAGATTTCCAACTATGAGA+AGG + chr8_2:69820198-69820217 Msa1265460:intron 35.0%
ATCAATCTCCTTCTCATAGT+TGG - chr8_2:69820209-69820228 None:intergenic 35.0%
!! AATTTGTTGCTTCTCTACGA+AGG + chr8_2:69820254-69820273 Msa1265460:CDS 35.0%
CTACTCTTCCTATAACATGA+AGG - chr8_2:69820380-69820399 None:intergenic 35.0%
TCATGTTATAGGAAGAGTAG+TGG + chr8_2:69820380-69820399 Msa1265460:intron 35.0%
GTCTGCATGCTAGTAATTTA+TGG + chr8_2:69820972-69820991 Msa1265460:intron 35.0%
!! AGGGTCTTGTTTAATAGGTA+AGG - chr8_2:69821322-69821341 None:intergenic 35.0%
!! GTAGAAGGGTCTTGTTTAAT+AGG - chr8_2:69821327-69821346 None:intergenic 35.0%
ATTATGGAAGCCGAGAATAT+TGG - chr8_2:69821361-69821380 None:intergenic 35.0%
AAAATCCATGTTGAATGGTG+TGG - chr8_2:69821406-69821425 None:intergenic 35.0%
GCAGAAAAATCCATGTTGAA+TGG - chr8_2:69821411-69821430 None:intergenic 35.0%
ACTGAAAAATGCTGCATTGT+GGG - chr8_2:69821466-69821485 None:intergenic 35.0%
CTGTTTCAAACGCCAATATT+TGG + chr8_2:69821696-69821715 Msa1265460:CDS 35.0%
TCAAACGCCAATATTTGGAA+TGG + chr8_2:69821701-69821720 Msa1265460:CDS 35.0%
GCTCATTCCATTCCAAATAT+TGG - chr8_2:69821711-69821730 None:intergenic 35.0%
!! GATGATGATGAAAGTGTTGA+AGG + chr8_2:69821798-69821817 Msa1265460:three_prime_UTR 35.0%
!! ATGATGAAAGTGTTGAAGGA+AGG + chr8_2:69821802-69821821 Msa1265460:three_prime_UTR 35.0%
TGCAAAGCAATAGAATCAGA+AGG + chr8_2:69821860-69821879 Msa1265460:three_prime_UTR 35.0%
ATGGTACCAACACAATGAAA+TGG - chr8_2:69822094-69822113 None:intergenic 35.0%
! GTGTTGTTAGTGTTTGTAGG+GGG - chr8_2:69818344-69818363 None:intergenic 40.0%
!! GGTGTTGTTAGTGTTTGTAG+GGG - chr8_2:69818345-69818364 None:intergenic 40.0%
!! GAGGTGTTGTTAGTGTTTGT+AGG - chr8_2:69818347-69818366 None:intergenic 40.0%
ATTGGAAAGAGAGAAAGCGA+GGG - chr8_2:69818369-69818388 None:intergenic 40.0%
TATTGGAAAGAGAGAAAGCG+AGG - chr8_2:69818370-69818389 None:intergenic 40.0%
! GATGGTGGTATTATAGAGGA+AGG - chr8_2:69818461-69818480 None:intergenic 40.0%
! GAGAGATGGTGGTATTATAG+AGG - chr8_2:69818465-69818484 None:intergenic 40.0%
ACAAACCTCTCCATATTCAC+CGG + chr8_2:69818647-69818666 Msa1265460:CDS 40.0%
GTGCATGTTTGAATTGACAG+TGG + chr8_2:69818934-69818953 Msa1265460:intron 40.0%
! TTTTCAGACATAGATGGACG+GGG + chr8_2:69819111-69819130 Msa1265460:intron 40.0%
GGAGATATGAAGCACATCTA+TGG + chr8_2:69819132-69819151 Msa1265460:CDS 40.0%
TATGGGACAATAGTTGCAGA+AGG + chr8_2:69819150-69819169 Msa1265460:CDS 40.0%
ATGGGACAATAGTTGCAGAA+GGG + chr8_2:69819151-69819170 Msa1265460:CDS 40.0%
CAAAGTAGGAAAGGAAGACA+AGG + chr8_2:69819179-69819198 Msa1265460:CDS 40.0%
ATCACAAACACTAGATCTCG+TGG - chr8_2:69819371-69819390 None:intergenic 40.0%
ACGAGATCTAGTGTTTGTGA+TGG + chr8_2:69819370-69819389 Msa1265460:intron 40.0%
CGAGATCTAGTGTTTGTGAT+GGG + chr8_2:69819371-69819390 Msa1265460:intron 40.0%
! ATCTAGTGTTTGTGATGGGA+AGG + chr8_2:69819375-69819394 Msa1265460:intron 40.0%
! TCTAGTGTTTGTGATGGGAA+GGG + chr8_2:69819376-69819395 Msa1265460:intron 40.0%
GATCAAAATAGATCGGGTGT+TGG + chr8_2:69819656-69819675 Msa1265460:intron 40.0%
! GAACTTTGAAGATTCTGACC+GGG + chr8_2:69819682-69819701 Msa1265460:intron 40.0%
!! GTGAATGATTGCTGATTCGT+GGG + chr8_2:69819728-69819747 Msa1265460:intron 40.0%
GTACAAACTTACCGGAAAGT+TGG - chr8_2:69820073-69820092 None:intergenic 40.0%
AAGAGTAGTGGTTTCTCAAG+AGG + chr8_2:69820392-69820411 Msa1265460:CDS 40.0%
!! AGAGGAGCATCAATTTACAG+AGG + chr8_2:69820410-69820429 Msa1265460:CDS 40.0%
!! GAGGAGCATCAATTTACAGA+GGG + chr8_2:69820411-69820430 Msa1265460:CDS 40.0%
!! AGGAGCATCAATTTACAGAG+GGG + chr8_2:69820412-69820431 Msa1265460:CDS 40.0%
CAATTTACAGAGGGGTAACA+AGG + chr8_2:69820420-69820439 Msa1265460:CDS 40.0%
! AAGGTAGAGGTCTTTGTTTC+CGG - chr8_2:69820633-69820652 None:intergenic 40.0%
!! AGTACATACTGAAAGTGCCA+AGG - chr8_2:69820652-69820671 None:intergenic 40.0%
TAGCATGCAGACGAACATTA+AGG - chr8_2:69820964-69820983 None:intergenic 40.0%
GCAAATAGTTCTCTTCCCAT+AGG + chr8_2:69821168-69821187 Msa1265460:CDS 40.0%
AATAGTTCTCTTCCCATAGG+AGG + chr8_2:69821171-69821190 Msa1265460:CDS 40.0%
TCATCCATCTTCAACTTCGT+TGG + chr8_2:69821278-69821297 Msa1265460:CDS 40.0%
! AAGACCCTTCTACCGATTAT+TGG + chr8_2:69821334-69821353 Msa1265460:CDS 40.0%
TGGACCAATAATCGGTAGAA+GGG - chr8_2:69821341-69821360 None:intergenic 40.0%
TTGGACCAATAATCGGTAGA+AGG - chr8_2:69821342-69821361 None:intergenic 40.0%
TACTACCACACCATTCAACA+TGG + chr8_2:69821398-69821417 Msa1265460:CDS 40.0%
ATTGTGGGGATTATCACTGT+TGG - chr8_2:69821451-69821470 None:intergenic 40.0%
CTGAAAAATGCTGCATTGTG+GGG - chr8_2:69821465-69821484 None:intergenic 40.0%
CACTGAAAAATGCTGCATTG+TGG - chr8_2:69821467-69821486 None:intergenic 40.0%
!! CACAATGCAGCATTTTTCAG+TGG + chr8_2:69821465-69821484 Msa1265460:CDS 40.0%
CAAAATATGAACGGAAGCAG+TGG + chr8_2:69821510-69821529 Msa1265460:CDS 40.0%
TGACGACGAAGATGAAGAAT+TGG - chr8_2:69821538-69821557 None:intergenic 40.0%
GAAAATAATGGCGTTGCGAA+TGG - chr8_2:69821579-69821598 None:intergenic 40.0%
! ATTGGCGTTTGAAACAGACT+TGG - chr8_2:69821693-69821712 None:intergenic 40.0%
GAAGATGAGTGGGAAGGATT+AGG - chr8_2:69818401-69818420 None:intergenic 45.0%
TGAAGGAGTGGAAAAGAGAG+AGG - chr8_2:69818430-69818449 None:intergenic 45.0%
GAGAGAGAGAGAGAGAGATA+GGG - chr8_2:69818510-69818529 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGAT+AGG - chr8_2:69818511-69818530 None:intergenic 45.0%
GGTGAATATGGAGAGGTTTG+TGG - chr8_2:69818648-69818667 None:intergenic 45.0%
CTAAGAAGTCCTCGAACTTG+TGG - chr8_2:69818676-69818695 None:intergenic 45.0%
CACAAGTTCGAGGACTTCTT+AGG + chr8_2:69818674-69818693 Msa1265460:CDS 45.0%
GAAAACTGCTGAAGCTGTGT+GGG - chr8_2:69818743-69818762 None:intergenic 45.0%
TTGAGGTTCGGAGTTTGGTT+CGG - chr8_2:69818837-69818856 None:intergenic 45.0%
!!! AGGAGAAGGCTTTTGAGGTT+CGG - chr8_2:69818849-69818868 None:intergenic 45.0%
GACAATAGTTGCAGAAGGGA+AGG + chr8_2:69819155-69819174 Msa1265460:CDS 45.0%
AGGGAAGGACAAAGTAGGAA+AGG + chr8_2:69819170-69819189 Msa1265460:CDS 45.0%
ATTGTGTCTAACGTGCGCTA+TGG + chr8_2:69819322-69819341 Msa1265460:intron 45.0%
TGACCACTGACCGGAATAAA+TGG + chr8_2:69819410-69819429 Msa1265460:intron 45.0%
GACCACTGACCGGAATAAAT+GGG + chr8_2:69819411-69819430 Msa1265460:intron 45.0%
TGACAGTGTGACTGATGCAT+TGG - chr8_2:69819581-69819600 None:intergenic 45.0%
CGAATCAGCAATCATTCACC+AGG - chr8_2:69819727-69819746 None:intergenic 45.0%
!! GGTGAATGATTGCTGATTCG+TGG + chr8_2:69819727-69819746 Msa1265460:intron 45.0%
ATCTTGCTGCTCTCAAGTAC+TGG + chr8_2:69820031-69820050 Msa1265460:CDS 45.0%
TCTTGCTGCTCTCAAGTACT+GGG + chr8_2:69820032-69820051 Msa1265460:CDS 45.0%
AACGACCACTACCAACTTTC+CGG + chr8_2:69820059-69820078 Msa1265460:CDS 45.0%
!! ACTTACCGGAAAGTTGGTAG+TGG - chr8_2:69820067-69820086 None:intergenic 45.0%
GGAAACAAAGACCTCTACCT+TGG + chr8_2:69820632-69820651 Msa1265460:CDS 45.0%
!! TACTGAAAGTGCCAAGGTAG+AGG - chr8_2:69820646-69820665 None:intergenic 45.0%
GGTTTCTGATGCAGAATCTG+TGG - chr8_2:69821223-69821242 None:intergenic 45.0%
GTTGCCAACGAAGTTGAAGA+TGG - chr8_2:69821285-69821304 None:intergenic 45.0%
CAGCACTGCTAGGGTTATTA+TGG - chr8_2:69821377-69821396 None:intergenic 45.0%
TGGTAGTAACAGCACTGCTA+GGG - chr8_2:69821386-69821405 None:intergenic 45.0%
!! GCAGCATTTTTCAGTGGATC+AGG + chr8_2:69821471-69821490 Msa1265460:CDS 45.0%
!! GACTTGGCTTTGCATGTGTT+TGG - chr8_2:69821677-69821696 None:intergenic 45.0%
GGAATGAGCTCATGCATATC+AGG + chr8_2:69821722-69821741 Msa1265460:exon 45.0%
!! TCATGCATATCAGGTGCTGA+TGG + chr8_2:69821731-69821750 Msa1265460:three_prime_UTR 45.0%
GAAGGACAGTGTTGTAGTGA+TGG + chr8_2:69821820-69821839 Msa1265460:three_prime_UTR 45.0%
!! TAAATAAAAAAATATTAGAT+GGG - chr8_2:69820764-69820783 None:intergenic 5.0%
!! ATAAATAAAAAAATATTAGA+TGG - chr8_2:69820765-69820784 None:intergenic 5.0%
!! AAAATTTATTATTGATATAT+TGG + chr8_2:69820886-69820905 Msa1265460:intron 5.0%
!!! TCAATTTAATTTATAAATTT+AGG + chr8_2:69821039-69821058 Msa1265460:intron 5.0%
! CGATGGCGTTGAAGCTTAGT+TGG - chr8_2:69818257-69818276 None:intergenic 50.0%
GTTTGTAGGGGGTGAAGAAG+AGG - chr8_2:69818333-69818352 None:intergenic 50.0%
TGAGAGGAAGATGAGTGGGA+AGG - chr8_2:69818407-69818426 None:intergenic 50.0%
GAGGTGAGAGGAAGATGAGT+GGG - chr8_2:69818411-69818430 None:intergenic 50.0%
AGAGGTGAGAGGAAGATGAG+TGG - chr8_2:69818412-69818431 None:intergenic 50.0%
GTGGAAAAGAGAGAGGTGAG+AGG - chr8_2:69818423-69818442 None:intergenic 50.0%
AAGGATAAGGGGTGAAGGAG+TGG - chr8_2:69818442-69818461 None:intergenic 50.0%
AGAGGAAGGATAAGGGGTGA+AGG - chr8_2:69818447-69818466 None:intergenic 50.0%
GGAGAGAGAAAGACCTGTGA+GGG - chr8_2:69818489-69818508 None:intergenic 50.0%
GGCTGTTCTTGTTGGTGCAA+CGG - chr8_2:69818705-69818724 None:intergenic 50.0%
GGAAAACTGCTGAAGCTGTG+TGG - chr8_2:69818744-69818763 None:intergenic 50.0%
ACAATAGCTGCTTGCTTCCC+TGG + chr8_2:69818800-69818819 Msa1265460:CDS 50.0%
!!! GGCTTTTGAGGTTCGGAGTT+TGG - chr8_2:69818842-69818861 None:intergenic 50.0%
!! GCGTTGTCCGAAGGTATCAA+CGG - chr8_2:69818879-69818898 None:intergenic 50.0%
GCAGAAGGGAAGGACAAAGT+AGG + chr8_2:69819165-69819184 Msa1265460:CDS 50.0%
TGTCTAACGTGCGCTATGGA+TGG + chr8_2:69819326-69819345 Msa1265460:intron 50.0%
GATGGTACACGGGCAAAAGT+GGG + chr8_2:69819344-69819363 Msa1265460:intron 50.0%
CAGTGAATGTGACCACTGAC+CGG + chr8_2:69819401-69819420 Msa1265460:intron 50.0%
CTCCCATTTATTCCGGTCAG+TGG - chr8_2:69819416-69819435 None:intergenic 50.0%
! CTTGCTGCTCTCAAGTACTG+GGG + chr8_2:69820033-69820052 Msa1265460:CDS 50.0%
!! GGAAAGTTGGTAGTGGTCGT+TGG - chr8_2:69820060-69820079 None:intergenic 50.0%
GTGGTAGTAACAGCACTGCT+AGG - chr8_2:69821387-69821406 None:intergenic 50.0%
GGAAAGAGAGAAAGCGAGGG+AGG - chr8_2:69818366-69818385 None:intergenic 55.0%
AGACCTGTGAGGGTGAGAGA+TGG - chr8_2:69818479-69818498 None:intergenic 55.0%
GGGAGAGAGAAAGACCTGTG+AGG - chr8_2:69818490-69818509 None:intergenic 55.0%
TGCCTCTGGTGAAGCTTCCA+CGG - chr8_2:69818612-69818631 None:intergenic 55.0%
GCTTCACCAGAGGCAACAGA+TGG + chr8_2:69818617-69818636 Msa1265460:CDS 55.0%
TCACCAGAGGCAACAGATGG+AGG + chr8_2:69818620-69818639 Msa1265460:CDS 55.0%
GTTCCTCCATCTGTTGCCTC+TGG - chr8_2:69818626-69818645 None:intergenic 55.0%
CTTGTGGCTGCCGGTGAATA+TGG - chr8_2:69818660-69818679 None:intergenic 55.0%
ATAGCTGCTTGCTTCCCTGG+TGG + chr8_2:69818803-69818822 Msa1265460:CDS 55.0%
!! GTTCGGAGTTTGGTTCGGTG+GGG - chr8_2:69818832-69818851 None:intergenic 55.0%
!! GGTTCGGAGTTTGGTTCGGT+GGG - chr8_2:69818833-69818852 None:intergenic 55.0%
!! AGGTTCGGAGTTTGGTTCGG+TGG - chr8_2:69818834-69818853 None:intergenic 55.0%
CAACGCACCTCCATCTATCG+CGG + chr8_2:69818893-69818912 Msa1265460:CDS 55.0%
CGTGCGCTATGGATGGTACA+CGG + chr8_2:69819333-69819352 Msa1265460:intron 55.0%
GTGCGCTATGGATGGTACAC+GGG + chr8_2:69819334-69819353 Msa1265460:intron 55.0%
GGATGGTACACGGGCAAAAG+TGG + chr8_2:69819343-69819362 Msa1265460:intron 55.0%
CGGTCAGTGGTCACATTCAC+TGG - chr8_2:69819403-69819422 None:intergenic 55.0%
TGCCTATCCTTGCCTGCCAT+CGG - chr8_2:69820604-69820623 None:intergenic 55.0%
GCAAGGATAGGCAGAGTTGC+CGG + chr8_2:69820611-69820630 Msa1265460:CDS 55.0%
CCTGTGAGGGTGAGAGATGG+TGG - chr8_2:69818476-69818495 None:intergenic 60.0%
CCACCATCTCTCACCCTCAC+AGG + chr8_2:69818473-69818492 Msa1265460:CDS 60.0%
GGCTGCCGGTGAATATGGAG+AGG - chr8_2:69818655-69818674 None:intergenic 60.0%
GTCCTCGAACTTGTGGCTGC+CGG - chr8_2:69818669-69818688 None:intergenic 60.0%
GTTGCGGCGGCTGTTCTTGT+TGG - chr8_2:69818713-69818732 None:intergenic 60.0%
CTGTGTGGGTGCACACGTTG+CGG - chr8_2:69818729-69818748 None:intergenic 60.0%
! GATGGAGGTGCGTTGTCCGA+AGG - chr8_2:69818888-69818907 None:intergenic 60.0%
GGTGACACCGCGATAGATGG+AGG - chr8_2:69818903-69818922 None:intergenic 60.0%
CCTGGTGACACCGCGATAGA+TGG - chr8_2:69818906-69818925 None:intergenic 60.0%
!! CCATCTATCGCGGTGTCACC+AGG + chr8_2:69818903-69818922 Msa1265460:CDS 60.0%
TGGTGCAGACACCACCAGCA+TGG + chr8_2:69820578-69820597 Msa1265460:intron 60.0%
TCCAGGAAGCAGCAACGCCG+TGG + chr8_2:69818592-69818611 Msa1265460:intron 65.0%
! TCCACGGCGTTGCTGCTTCC+TGG - chr8_2:69818596-69818615 None:intergenic 65.0%
CGCCGTGGAAGCTTCACCAG+AGG + chr8_2:69818607-69818626 Msa1265460:CDS 65.0%
CACCGGCAGCCACAAGTTCG+AGG + chr8_2:69818664-69818683 Msa1265460:CDS 65.0%
TGTGGGTGCACACGTTGCGG+CGG - chr8_2:69818726-69818745 None:intergenic 65.0%
! GTTCGGTGGGGTAGCCACCA+GGG - chr8_2:69818820-69818839 None:intergenic 65.0%
GACACCACCAGCATGGCCGA+TGG + chr8_2:69820585-69820604 Msa1265460:intron 65.0%
TTGCCTGCCATCGGCCATGC+TGG - chr8_2:69820595-69820614 None:intergenic 65.0%
AGCATGGCCGATGGCAGGCA+AGG + chr8_2:69820594-69820613 Msa1265460:CDS 65.0%
GGCCGATGGCAGGCAAGGAT+AGG + chr8_2:69820599-69820618 Msa1265460:CDS 65.0%
!! GGTTCGGTGGGGTAGCCACC+AGG - chr8_2:69818821-69818840 None:intergenic 70.0%
CCTGCCATCGGCCATGCTGG+TGG - chr8_2:69820592-69820611 None:intergenic 70.0%
CCACCAGCATGGCCGATGGC+AGG + chr8_2:69820589-69820608 Msa1265460:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 69818214 69822189 69818214 ID=Msa1265460;Name=Msa1265460
chr8_2 mRNA 69818214 69822189 69818214 ID=Msa1265460-mRNA-1;Parent=Msa1265460;Name=Msa1265460-mRNA-1;_AED=0.14;_eAED=0.14;_QI=106|0.85|1|1|1|1|8|461|514
chr8_2 exon 69818214 69818494 69818214 ID=Msa1265460-mRNA-1:exon:23090;Parent=Msa1265460-mRNA-1
chr8_2 exon 69818597 69818924 69818597 ID=Msa1265460-mRNA-1:exon:23091;Parent=Msa1265460-mRNA-1
chr8_2 exon 69819118 69819200 69819118 ID=Msa1265460-mRNA-1:exon:23092;Parent=Msa1265460-mRNA-1
chr8_2 exon 69819992 69820080 69819992 ID=Msa1265460-mRNA-1:exon:23093;Parent=Msa1265460-mRNA-1
chr8_2 exon 69820202 69820275 69820202 ID=Msa1265460-mRNA-1:exon:23094;Parent=Msa1265460-mRNA-1
chr8_2 exon 69820391 69820441 69820391 ID=Msa1265460-mRNA-1:exon:23095;Parent=Msa1265460-mRNA-1
chr8_2 exon 69820586 69820662 69820586 ID=Msa1265460-mRNA-1:exon:23096;Parent=Msa1265460-mRNA-1
chr8_2 exon 69821061 69822189 69821061 ID=Msa1265460-mRNA-1:exon:23097;Parent=Msa1265460-mRNA-1
chr8_2 five_prime_UTR 69818214 69818319 69818214 ID=Msa1265460-mRNA-1:five_prime_utr;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69818320 69818494 69818320 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69818597 69818924 69818597 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69819118 69819200 69819118 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69819992 69820080 69819992 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69820202 69820275 69820202 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69820391 69820441 69820391 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69820586 69820662 69820586 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 CDS 69821061 69821728 69821061 ID=Msa1265460-mRNA-1:cds;Parent=Msa1265460-mRNA-1
chr8_2 three_prime_UTR 69821729 69822189 69821729 ID=Msa1265460-mRNA-1:three_prime_utr;Parent=Msa1265460-mRNA-1
Gene Sequence

>Msa1265460

ATGGACAAATCCTCTTCTTCACCCCCTACAAACACTAACAACACCTCCCTCGCTTTCTCTCTTTCCAATAATAACTTTCCTAATCCTTCCCACTCATCTTCCTCTCACCTCTCTCTTTTCCACTCCTTCACCCCTTATCCTTCCTCTATAATACCACCATCTCTCACCCTCACAGGAAGCAGCAACGCCGTGGAAGCTTCACCAGAGGCAACAGATGGAGGAACCACAAACCTCTCCATATTCACCGGCAGCCACAAGTTCGAGGACTTCTTAGGAAGCTCCGTTGCACCAACAAGAACAGCCGCCGCAACGTGTGCACCCACACAGCTTCAGCAGTTTTCCACCGACAATGATATATATAATTCCGAGCTGAAGAAAACAATAGCTGCTTGCTTCCCTGGTGGCTACCCCACCGAACCAAACTCCGAACCTCAAAAGCCTTCTCCTAAAAAAACCGTTGATACCTTCGGACAACGCACCTCCATCTATCGCGGTGTCACCAGACATAGATGGACGGGGAGATATGAAGCACATCTATGGGACAATAGTTGCAGAAGGGAAGGACAAAGTAGGAAAGGAAGACAAGGTGGCTATGATAAGGAAGAGAAAGCAGCTAGAGCTTATGATCTTGCTGCTCTCAAGTACTGGGGTCCAACGACCACTACCAACTTTCCGATTTCCAACTATGAGAAGGAGATTGATGACATGAAGAACATGACTAGACAAGAATTTGTTGCTTCTCTACGAAGGAAGAGTAGTGGTTTCTCAAGAGGAGCATCAATTTACAGAGGGGTAACAAGACACCACCAGCATGGCCGATGGCAGGCAAGGATAGGCAGAGTTGCCGGAAACAAAGACCTCTACCTTGGCACTTTCAGCACTCAAGAAGAAGCTGCAGAAGCGTATGATATTGCAGCAATCAAATTTAGAGGACTAAATGCAGTAACAAATTTTGACATGAGTCGCTACGACGTAAAGAGTATTGCAAATAGTTCTCTTCCCATAGGAGGTTTATCAAATAAGAACAACAAAAATTCCACAGATTCTGCATCAGAAACCAAAATCAACGAACCAATTCAATCTGACGAAATAGATCATCCATCTTCAACTTCGTTGGCAACAACTTTAAGCTTCGCCTTACCTATTAAACAAGACCCTTCTACCGATTATTGGTCCAATATTCTCGGCTTCCATAATAACCCTAGCAGTGCTGTTACTACCACACCATTCAACATGGATTTTTCTGCTCATGCACCTTCAAATACCAACAGTGATAATCCCCACAATGCAGCATTTTTCAGTGGATCAGGTATATTTTTTCAGCAACAAAATATGAACGGAAGCAGTGGTTCCAATTCTTCATCTTCGTCGTCAGCTTCAACGAGTTCAATTCCATTCGCAACGCCATTATTTTCTTTAAATAGTAATAGTAGTAGTTATGAGAATGGTACTAGTTATGGAAATGGAAATAACTGGATTGTACACACATTCCAAACACATGCAAAGCCAAGTCTGTTTCAAACGCCAATATTTGGAATGGAATGA
Protein sequence

>Msa1265460

MDKSSSSPPTNTNNTSLAFSLSNNNFPNPSHSSSSHLSLFHSFTPYPSSIIPPSLTLTGSSNAVEASPEATDGGTTNLSIFTGSHKFEDFLGSSVAPTRTAAATCAPTQLQQFSTDNDIYNSELKKTIAACFPGGYPTEPNSEPQKPSPKKTVDTFGQRTSIYRGVTRHRWTGRYEAHLWDNSCRREGQSRKGRQGGYDKEEKAARAYDLAALKYWGPTTTTNFPISNYEKEIDDMKNMTRQEFVASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDIAAIKFRGLNAVTNFDMSRYDVKSIANSSLPIGGLSNKNNKNSTDSASETKINEPIQSDEIDHPSSTSLATTLSFALPIKQDPSTDYWSNILGFHNNPSSAVTTTPFNMDFSAHAPSNTNSDNPHNAAFFSGSGIFFQQQNMNGSSGSNSSSSSSASTSSIPFATPLFSLNSNSSSYENGTSYGNGNNWIVHTFQTHAKPSLFQTPIFGME*