AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1221130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221130 MtrunA17_Chr4g0072291 95.560 518 13 3 1 514 1 512 0.0 1003
Msa1221130 MtrunA17_Chr2g0328971 87.958 191 22 1 148 337 156 346 6.77e-125 375
Msa1221130 MtrunA17_Chr7g0249621 88.325 197 19 2 149 341 249 445 6.18e-124 378
Msa1221130 MtrunA17_Chr4g0032631 88.601 193 18 2 149 337 153 345 8.45e-124 373
Msa1221130 MtrunA17_Chr8g0343851 83.673 196 32 0 142 337 231 426 3.35e-120 365
Msa1221130 MtrunA17_Chr1g0152161 62.921 267 87 2 91 357 256 510 1.05e-114 353
Msa1221130 MtrunA17_Chr3g0135161 57.843 306 107 5 91 395 254 538 8.29e-114 351
Msa1221130 MtrunA17_Chr4g0054351 67.078 243 76 2 143 382 294 535 2.96e-111 344
Msa1221130 MtrunA17_Chr5g0412151 84.264 197 28 1 148 341 213 409 3.06e-111 340
Msa1221130 MtrunA17_Chr5g0400871 81.111 180 31 1 155 331 245 424 6.54e-107 329
Msa1221130 MtrunA17_Chr4g0073521 72.892 166 45 0 158 323 46 211 9.95e-89 276
Msa1221130 MtrunA17_Chr7g0216601 63.298 188 66 1 139 323 45 232 3.07e-80 256
Msa1221130 MtrunA17_Chr8g0367311 59.048 210 76 4 196 402 22 224 9.77e-79 247
Msa1221130 MtrunA17_Chr6g0452911 66.667 171 47 1 163 323 65 235 1.21e-78 251
Msa1221130 MtrunA17_Chr8g0355191 68.072 166 53 0 158 323 54 219 3.25e-78 251
Msa1221130 MtrunA17_Chr4g0002011 70.414 169 47 1 158 323 35 203 1.03e-76 244
Msa1221130 MtrunA17_Chr8g0355181 66.867 166 55 0 158 323 55 220 5.30e-73 236
Msa1221130 MtrunA17_Chr8g0362541 62.245 196 60 1 142 323 52 247 1.42e-72 236
Msa1221130 MtrunA17_Chr2g0305821 67.857 112 36 0 158 269 46 157 4.43e-44 153
Msa1221130 MtrunA17_Chr7g0257361 61.538 130 42 2 157 283 55 179 4.20e-42 155
Msa1221130 MtrunA17_Chr4g0053181 49.239 197 84 5 163 356 177 360 3.01e-40 152
Msa1221130 MtrunA17_Chr7g0263581 47.396 192 92 4 134 324 110 293 3.37e-40 151
Msa1221130 MtrunA17_Chr5g0401891 53.086 162 68 3 163 324 153 306 9.39e-40 150
Msa1221130 MtrunA17_Chr4g0030191 49.091 165 70 4 163 324 132 285 2.98e-37 143
Msa1221130 MtrunA17_Chr2g0325911 45.556 180 75 4 145 324 117 273 3.59e-32 128
Msa1221130 MtrunA17_Chr2g0305831 76.271 59 14 0 265 323 1 59 6.27e-26 105
Msa1221130 MtrunA17_Chr3g0126151 45.263 95 51 1 207 301 47 140 4.02e-22 92.4
Msa1221130 MtrunA17_Chr8g0367321 78.000 50 11 0 148 197 144 193 1.05e-21 93.2
Msa1221130 MtrunA17_Chr1g0170761 36.691 139 74 4 143 278 118 245 3.24e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1221130 AT5G57390.1 53.799 487 159 13 75 514 91 558 1.07e-147 435
Msa1221130 AT5G57390.2 57.243 428 138 10 118 514 27 440 4.85e-145 424
Msa1221130 AT1G51190.1 85.500 200 25 2 146 341 174 373 1.01e-123 374
Msa1221130 AT3G20840.1 86.082 194 23 2 148 337 167 360 7.06e-122 369
Msa1221130 AT5G17430.2 86.154 195 23 2 146 336 132 326 1.17e-120 364
Msa1221130 AT5G17430.1 86.154 195 23 2 146 336 194 388 4.38e-120 365
Msa1221130 AT1G72570.1 83.158 190 29 1 149 335 210 399 7.13e-116 348
Msa1221130 AT4G37750.1 64.130 276 83 7 150 412 271 543 1.78e-113 347
Msa1221130 AT5G10510.2 73.593 231 50 1 121 351 200 419 3.67e-110 338
Msa1221130 AT5G10510.7 72.650 234 50 2 121 351 200 422 5.80e-108 332
Msa1221130 AT5G10510.5 72.650 234 50 2 121 351 200 422 5.80e-108 332
Msa1221130 AT5G65510.4 73.451 226 46 2 121 343 143 357 6.54e-108 329
Msa1221130 AT5G10510.1 72.650 234 50 2 121 351 223 445 9.89e-108 333
Msa1221130 AT5G10510.6 71.074 242 54 3 121 359 238 466 1.41e-107 333
Msa1221130 AT5G10510.4 71.074 242 54 3 121 359 238 466 1.41e-107 333
Msa1221130 AT5G65510.2 73.451 226 46 2 121 343 201 415 1.61e-107 330
Msa1221130 AT5G65510.1 73.451 226 46 2 121 343 201 415 1.61e-107 330
Msa1221130 AT5G10510.3 69.583 240 48 2 143 359 249 486 3.07e-103 322
Msa1221130 AT5G65510.3 74.857 175 30 2 121 292 143 306 8.78e-91 280
Msa1221130 AT3G54320.3 67.456 169 52 1 158 323 61 229 2.50e-70 231
Msa1221130 AT3G54320.1 67.456 169 52 1 158 323 61 229 2.61e-70 231
Msa1221130 AT1G16060.1 69.231 169 49 1 158 323 54 222 2.39e-65 216
Msa1221130 AT1G79700.2 68.639 169 50 1 158 323 48 216 5.45e-65 213
Msa1221130 AT1G79700.1 64.497 169 47 2 158 323 48 206 4.82e-57 192
Msa1221130 AT3G54320.4 68.750 128 40 0 196 323 36 163 2.01e-51 179
Msa1221130 AT3G54320.5 68.750 128 40 0 196 323 36 163 2.01e-51 179
Msa1221130 AT1G79700.3 69.531 128 39 0 196 323 13 140 9.27e-46 160
Msa1221130 AT1G79700.4 69.531 128 39 0 196 323 7 134 9.41e-46 160
Msa1221130 AT1G16060.3 68.657 134 42 0 190 323 19 152 2.46e-45 160
Msa1221130 AT2G41710.1 59.848 132 45 2 155 283 63 189 1.57e-41 154
Msa1221130 AT2G41710.4 59.848 132 45 2 155 283 63 189 3.69e-41 154
Msa1221130 AT4G36920.1 50.259 193 81 5 143 332 113 293 7.25e-41 152
Msa1221130 AT4G36920.2 50.259 193 81 5 143 332 113 293 7.25e-41 152
Msa1221130 AT2G41710.2 57.664 137 45 3 155 283 63 194 4.54e-39 147
Msa1221130 AT2G28550.3 39.544 263 122 7 163 407 154 397 4.69e-39 148
Msa1221130 AT5G67180.2 46.842 190 90 4 135 324 72 250 8.28e-38 141
Msa1221130 AT5G67180.3 46.842 190 90 4 135 324 72 250 1.28e-37 140
Msa1221130 AT5G67180.1 47.150 193 85 5 135 324 72 250 3.79e-37 140
Msa1221130 AT2G41710.3 47.305 167 45 3 155 283 63 224 9.38e-35 136
Msa1221130 AT1G16060.2 72.727 88 24 0 236 323 1 88 5.79e-33 125
Msa1221130 AT1G16060.4 72.727 88 24 0 236 323 1 88 5.79e-33 125
Msa1221130 AT1G16060.6 72.727 88 24 0 236 323 1 88 5.79e-33 125
Msa1221130 AT1G16060.5 72.727 88 24 0 236 323 1 88 5.79e-33 125
Msa1221130 AT3G54320.2 71.591 88 25 0 236 323 1 88 5.37e-32 124
Msa1221130 AT2G28550.1 36.882 263 114 8 163 407 154 382 1.14e-30 124
Msa1221130 AT2G28550.4 48.571 140 58 4 163 299 154 282 5.44e-30 120
Msa1221130 AT5G67180.4 44.118 170 84 4 135 304 72 230 2.23e-28 114
Msa1221130 AT2G28550.2 48.413 126 51 4 163 285 154 268 3.79e-25 105
Msa1221130 AT2G28550.6 48.413 126 51 4 163 285 154 268 3.79e-25 105
Msa1221130 AT2G28550.5 35.683 227 107 5 196 407 14 216 1.73e-23 100
Msa1221130 AT2G39250.2 42.857 112 51 3 163 271 109 210 1.60e-22 96.7
Msa1221130 AT2G39250.1 42.857 112 51 3 163 271 109 210 3.98e-22 97.8
Msa1221130 AT2G39250.1 40.196 102 55 3 235 332 80 179 9.17e-11 63.5
Msa1221130 AT5G60120.2 29.752 363 188 18 138 480 140 455 3.17e-19 91.3
Msa1221130 AT5G60120.1 37.569 181 80 7 139 316 141 291 6.14e-19 90.1
Msa1221130 AT5G60120.6 37.363 182 81 7 138 316 140 291 6.56e-19 90.1
Msa1221130 AT1G16060.7 79.310 58 12 0 266 323 9 66 6.58e-19 85.1
Msa1221130 AT5G60120.4 42.105 152 66 6 138 284 140 274 7.38e-19 89.4
Msa1221130 AT5G60120.3 42.105 152 66 6 138 284 140 274 7.38e-19 89.4
Msa1221130 AT5G60120.5 42.105 152 66 6 138 284 140 274 7.38e-19 89.4
Msa1221130 AT3G54990.2 47.170 106 43 3 163 265 123 218 2.31e-17 82.0
Msa1221130 AT3G54990.1 47.170 106 43 3 163 265 123 218 6.73e-17 82.4
Msa1221130 AT5G65130.1 32.571 175 94 6 250 405 99 268 5.60e-11 63.9
Msa1221130 AT5G65130.2 32.571 175 94 6 250 405 101 270 5.77e-11 63.9
Msa1221130 AT1G36060.1 47.368 76 39 1 252 327 133 207 5.83e-11 64.3
Msa1221130 AT1G03800.1 40.860 93 54 1 231 323 22 113 6.10e-11 63.2

Find 157 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTTCAAACGCCAATATT+TGG 0.167429 8_1:+84629868 Msa1221130:CDS
TTGAATTGGTTCGTTGATTT+TGG 0.203511 8_1:-84629413 None:intergenic
AGGATTAGGAAAGTTATTAT+TGG 0.265279 8_1:-84626557 None:intergenic
AAGACCCTTCTACCGATTAT+TGG 0.267990 8_1:+84629506 Msa1221130:CDS
TGAAGCTTAGTTGGTTTCTT+TGG 0.274376 8_1:-84626418 None:intergenic
CAGCACTGCTAGGGTTATTA+TGG 0.274613 8_1:-84629546 None:intergenic
GTAGAAGGGTCTTGTTTAAT+AGG 0.278427 8_1:-84629496 None:intergenic
GCTCATTCCATTCCAAATAT+TGG 0.285129 8_1:-84629880 None:intergenic
TTACTATTTAAAGAAAATAT+TGG 0.298151 8_1:-84629760 None:intergenic
ATCTTGCTGCTCTCAAGTAC+TGG 0.314872 8_1:+84628203 Msa1221130:CDS
CTTATTTGATAAACCTCCTA+TGG 0.315963 8_1:-84629356 None:intergenic
AAGGTAGAGGTCTTTGTTTC+CGG 0.318164 8_1:-84628802 None:intergenic
GGAGATATGAAGCACATCTA+TGG 0.326305 8_1:+84627304 Msa1221130:CDS
CTTGTGGCTGCCGGTGAATA+TGG 0.331498 8_1:-84626828 None:intergenic
AACGACCACTACCAACTTTC+CGG 0.338959 8_1:+84628231 Msa1221130:CDS
CACAAGTTCGAGGACTTCTT+AGG 0.357176 8_1:+84626845 Msa1221130:CDS
ATTGCAGCAATCAAATTTAG+AGG 0.358044 8_1:+84629265 Msa1221130:CDS
GAGGTGTTGTTAGTGTTTGT+AGG 0.362481 8_1:-84626517 None:intergenic
GAAGATGAGTGGGAAGGATT+AGG 0.362867 8_1:-84626571 None:intergenic
GACATGATTTATGAGTATAA+TGG 0.370754 8_1:+84630224 Msa1221130:three_prime_UTR
TTGGACCAATAATCGGTAGA+AGG 0.379956 8_1:-84629511 None:intergenic
TATGAGAATGGTACTAGTTA+TGG 0.381199 8_1:+84629793 Msa1221130:CDS
GGTATTATAGAGGAAGGATA+AGG 0.383296 8_1:-84626625 None:intergenic
ATTATGGAAGCCGAGAATAT+TGG 0.385997 8_1:-84629530 None:intergenic
ACATGATTTATGAGTATAAT+GGG 0.387504 8_1:+84630225 Msa1221130:three_prime_UTR
CCTGGTGACACCGCGATAGA+TGG 0.390623 8_1:-84627074 None:intergenic
CGATGGCGTTGAAGCTTAGT+TGG 0.395432 8_1:-84626427 None:intergenic
GACTTGGCTTTGCATGTGTT+TGG 0.396154 8_1:-84629846 None:intergenic
GGAATGAGCTCATGCATATC+AGG 0.398293 8_1:+84629894 Msa1221130:exon
AGGTGTTGTTAGTGTTTGTA+GGG 0.402140 8_1:-84626516 None:intergenic
TCTATTTCGTCAGATTGAAT+TGG 0.402860 8_1:-84629427 None:intergenic
ATCAATCTCCTTCTCATAGT+TGG 0.408692 8_1:-84628378 None:intergenic
GTATTATAGAGGAAGGATAA+GGG 0.409045 8_1:-84626624 None:intergenic
AATGGTACTAGTTATGGAAA+TGG 0.413030 8_1:+84629799 Msa1221130:CDS
ATGGTACCAACACAATGAAA+TGG 0.414251 8_1:-84630267 None:intergenic
GATTATTGGTCCAATATTCT+CGG 0.416679 8_1:+84629520 Msa1221130:CDS
GTTATGGAAATGGAAATAAC+TGG 0.419371 8_1:+84629809 Msa1221130:CDS
ACAATAGCTGCTTGCTTCCC+TGG 0.422931 8_1:+84626971 Msa1221130:CDS
TGTAATCCATTTCATTGTGT+TGG 0.424726 8_1:+84630261 Msa1221130:three_prime_UTR
TTGCCTGCCATCGGCCATGC+TGG 0.430696 8_1:-84628764 None:intergenic
GGTTCGGTGGGGTAGCCACC+AGG 0.431604 8_1:-84626989 None:intergenic
GAGAATATTGGACCAATAAT+CGG 0.435797 8_1:-84629518 None:intergenic
TTATCACTGTTGGTATTTGA+AGG 0.440953 8_1:-84629610 None:intergenic
TGACGACGAAGATGAAGAAT+TGG 0.441913 8_1:-84629707 None:intergenic
GTTGCGGCGGCTGTTCTTGT+TGG 0.443667 8_1:-84626881 None:intergenic
AGGTTCGGAGTTTGGTTCGG+TGG 0.444923 8_1:-84627002 None:intergenic
GAGAGATGGTGGTATTATAG+AGG 0.451776 8_1:-84626635 None:intergenic
GCAAGAATAGGCAGAGTTGC+CGG 0.451955 8_1:+84628783 Msa1221130:CDS
TAATTTAGGTGGCTATGATA+AGG 0.452771 8_1:+84628156 Msa1221130:intron
GGTTCGGAGTTTGGTTCGGT+GGG 0.458866 8_1:-84627001 None:intergenic
TTGAGGTTCGGAGTTTGGTT+CGG 0.459991 8_1:-84627005 None:intergenic
TCTTGCTGCTCTCAAGTACT+GGG 0.460585 8_1:+84628204 Msa1221130:CDS
GGCTGTTCTTGTTGGTGCAA+CGG 0.461915 8_1:-84626873 None:intergenic
AAGGATAAGGGGTGAAGGAG+TGG 0.462972 8_1:-84626612 None:intergenic
GGAAATGGAAATAACTGGAT+TGG 0.463612 8_1:+84629814 Msa1221130:CDS
GATGGTGGTATTATAGAGGA+AGG 0.464335 8_1:-84626631 None:intergenic
GAAAATATTGGTGTTGCGAA+TGG 0.474020 8_1:-84629748 None:intergenic
CCATCTATCGCGGTGTCACC+AGG 0.475525 8_1:+84627074 Msa1221130:CDS
GTTCCTCCATCTGTTGCCTC+TGG 0.480278 8_1:-84626794 None:intergenic
AGGGTCTTGTTTAATAGGTA+AGG 0.484892 8_1:-84629491 None:intergenic
GATGATGATGAAAGTGTTGA+AGG 0.487520 8_1:+84629970 Msa1221130:three_prime_UTR
GAGGTGAGAGGAAGATGAGT+GGG 0.487978 8_1:-84626581 None:intergenic
AGAGGAAGGATAAGGGGTGA+AGG 0.490294 8_1:-84626617 None:intergenic
ACAATGAAATGGATTACAAA+AGG 0.493633 8_1:-84630256 None:intergenic
GTCCTCGAACTTGTGGCTGC+CGG 0.496373 8_1:-84626837 None:intergenic
TCAAACGCCAATATTTGGAA+TGG 0.498126 8_1:+84629873 Msa1221130:CDS
AATAGTAGTAGTTATGAGAA+TGG 0.502038 8_1:+84629781 Msa1221130:CDS
TATTGGAAAGAGAGAAAGCG+AGG 0.505616 8_1:-84626540 None:intergenic
GGTGAATATGGAGAGGTTTG+TGG 0.510555 8_1:-84626816 None:intergenic
GGCCGATGGCAGGCAAGAAT+AGG 0.512416 8_1:+84628771 Msa1221130:CDS
GTGGTAGTAACAGCACTGCT+AGG 0.512894 8_1:-84629556 None:intergenic
GGAAAGAGAGAAAGCGAGGG+AGG 0.518672 8_1:-84626536 None:intergenic
TGAAGGAGTGGAAAAGAGAG+AGG 0.524119 8_1:-84626600 None:intergenic
TGCCTCTGGTGAAGCTTCCA+CGG 0.528484 8_1:-84626780 None:intergenic
AATAGTTCTCTTCCCATAGG+AGG 0.530212 8_1:+84629343 Msa1221130:CDS
GAAAACTGCTGAAGCTGTGT+GGG 0.532869 8_1:-84626911 None:intergenic
CCACCAGCATGGCCGATGGC+AGG 0.533190 8_1:+84628761 Msa1221130:CDS
TACTGAAAGTGCCAAGGTAG+AGG 0.534747 8_1:-84628815 None:intergenic
CTAAGAAGTCCTCGAACTTG+TGG 0.537216 8_1:-84626844 None:intergenic
CCTGCCATCGGCCATGCTGG+TGG 0.544113 8_1:-84628761 None:intergenic
TGCCTATTCTTGCCTGCCAT+CGG 0.545171 8_1:-84628773 None:intergenic
TGAAAGTGTTGAAGGAAGGA+AGG 0.546554 8_1:+84629978 Msa1221130:three_prime_UTR
GGAATTATATATATCATTGT+CGG 0.547335 8_1:-84626936 None:intergenic
GTGGAAAAGAGAGAGGTGAG+AGG 0.548112 8_1:-84626593 None:intergenic
ATTGGAAAGAGAGAAAGCGA+GGG 0.549140 8_1:-84626539 None:intergenic
AGAGGTGAGAGGAAGATGAG+TGG 0.549550 8_1:-84626582 None:intergenic
GCAAATAGTTCTCTTCCCAT+AGG 0.555242 8_1:+84629340 Msa1221130:CDS
CCACCATCTCTCACCCTCAC+AGG 0.557858 8_1:+84626646 Msa1221130:CDS
TGAGAGGAAGATGAGTGGGA+AGG 0.558701 8_1:-84626577 None:intergenic
GAGATATGAAGCACATCTAT+GGG 0.559005 8_1:+84627305 Msa1221130:CDS
CAAAATATGAACGGAAGCAG+TGG 0.559047 8_1:+84629682 Msa1221130:CDS
AGGGAAGGACAAAGTAGGAA+AGG 0.559779 8_1:+84627342 Msa1221130:CDS
GTACAAACTTACCGGAAAGT+TGG 0.561227 8_1:-84628242 None:intergenic
CAATTTACAGAGGGGTAACA+AGG 0.562932 8_1:+84628592 Msa1221130:CDS
TTATTTGATAAACCTCCTAT+GGG 0.563688 8_1:-84629355 None:intergenic
GTTGCCGACGAAGTTGAAGA+TGG 0.564476 8_1:-84629454 None:intergenic
TGGTAGTAACAGCACTGCTA+GGG 0.567073 8_1:-84629555 None:intergenic
CACCGGCAGCCACAAGTTCG+AGG 0.568522 8_1:+84626835 Msa1221130:CDS
GGAAAGTTGGTAGTGGTCGT+TGG 0.570366 8_1:-84628229 None:intergenic
GGAAACAAAGACCTCTACCT+TGG 0.572572 8_1:+84628804 Msa1221130:CDS
GTGTTGTTAGTGTTTGTAGG+GGG 0.575757 8_1:-84626514 None:intergenic
AAGAGTAGTGGTTTCTCAAG+AGG 0.576512 8_1:+84628564 Msa1221130:CDS
GATGGAGGTGCGTTGTCCGA+AGG 0.579181 8_1:-84627056 None:intergenic
GCTTCACCAGAGGCAACAGA+TGG 0.579683 8_1:+84626788 Msa1221130:CDS
TACTACCACACCATTCAACA+TGG 0.582642 8_1:+84629570 Msa1221130:CDS
TTTCAGCAACAAAATATGAA+CGG 0.583240 8_1:+84629673 Msa1221130:CDS
TCAGATTTCCAACTATGAGA+AGG 0.583626 8_1:+84628370 Msa1221130:intron
TATGGGACAATAGTTGCAGA+AGG 0.584131 8_1:+84627322 Msa1221130:CDS
GACACCACCAGCATGGCCGA+TGG 0.584738 8_1:+84628757 Msa1221130:intron
GACAATAGTTGCAGAAGGGA+AGG 0.585408 8_1:+84627327 Msa1221130:CDS
TATTATAGAGGAAGGATAAG+GGG 0.586147 8_1:-84626623 None:intergenic
TCATGCATATCAGGTGCTGA+TGG 0.589848 8_1:+84629903 Msa1221130:three_prime_UTR
GGAGAGAGAAAGACCTGTGA+GGG 0.590470 8_1:-84626659 None:intergenic
TGGACCAATAATCGGTAGAA+GGG 0.591088 8_1:-84629510 None:intergenic
TCATCCATCTTCAACTTCGT+CGG 0.591398 8_1:+84629450 Msa1221130:CDS
ATATGAACGTGAAATATCGA+TGG 0.594272 8_1:-84626444 None:intergenic
CAAAGTAGGAAAGGAAGACA+AGG 0.595973 8_1:+84627351 Msa1221130:CDS
GAAGGACAGTGTTGTAGTGA+TGG 0.596070 8_1:+84629996 Msa1221130:three_prime_UTR
GCAGAAGGGAAGGACAAAGT+AGG 0.597553 8_1:+84627337 Msa1221130:CDS
GGTGTTGTTAGTGTTTGTAG+GGG 0.599101 8_1:-84626515 None:intergenic
GTTCGGAGTTTGGTTCGGTG+GGG 0.599227 8_1:-84627000 None:intergenic
GGTTTCTGATGCAGAATCTG+TGG 0.599958 8_1:-84629392 None:intergenic
TCATGTTATAGGAAGAGTAG+TGG 0.600719 8_1:+84628552 Msa1221130:intron
ATTGGCGTTTGAAACAGACT+TGG 0.602086 8_1:-84629862 None:intergenic
GTTCGGTGGGGTAGCCACCA+GGG 0.604192 8_1:-84626988 None:intergenic
ATTATATATATCATTGTCGG+TGG 0.604777 8_1:-84626933 None:intergenic
CTGTGTGGGTGCACACGTTG+CGG 0.605215 8_1:-84626897 None:intergenic
GAGGAGCATCAATTTACAGA+GGG 0.606917 8_1:+84628583 Msa1221130:CDS
ATTGTGGGGATTATCACTGT+TGG 0.607060 8_1:-84629620 None:intergenic
AATTTGTTGCTTCTCTACGA+AGG 0.609124 8_1:+84628426 Msa1221130:CDS
ACAAACCTCTCCATATTCAC+CGG 0.611418 8_1:+84626818 Msa1221130:CDS
ATGATGAAAGTGTTGAAGGA+AGG 0.612896 8_1:+84629974 Msa1221130:three_prime_UTR
TCACCAGAGGCAACAGATGG+AGG 0.617655 8_1:+84626791 Msa1221130:CDS
TGCAAAGCAATAGAATCAGA+AGG 0.621670 8_1:+84630036 Msa1221130:three_prime_UTR
AATAGAATCAGAAGGAAACA+AGG 0.627213 8_1:+84630044 Msa1221130:three_prime_UTR
TCCAGGAAGCAGCAACGCCG+TGG 0.632938 8_1:+84626763 Msa1221130:intron
CGCCGTGGAAGCTTCACCAG+AGG 0.633581 8_1:+84626778 Msa1221130:CDS
CCTGTGAGGGTGAGAGATGG+TGG 0.636945 8_1:-84626646 None:intergenic
GGAAAACTGCTGAAGCTGTG+TGG 0.638121 8_1:-84626912 None:intergenic
TGTGGGTGCACACGTTGCGG+CGG 0.646698 8_1:-84626894 None:intergenic
TGGTGCAGACACCACCAGCA+TGG 0.647953 8_1:+84628750 Msa1221130:intron
AAAATCCATGTTGAATGGTG+TGG 0.648019 8_1:-84629575 None:intergenic
ACTTACCGGAAAGTTGGTAG+TGG 0.649056 8_1:-84628236 None:intergenic
ATGGGACAATAGTTGCAGAA+GGG 0.651127 8_1:+84627323 Msa1221130:CDS
GCGTTGTCCGAAGGTATCAA+CGG 0.652510 8_1:-84627047 None:intergenic
AGTACATACTGAAAGTGCCA+AGG 0.656101 8_1:-84628821 None:intergenic
GGTGACACCGCGATAGATGG+AGG 0.661765 8_1:-84627071 None:intergenic
AGACCTGTGAGGGTGAGAGA+TGG 0.662144 8_1:-84626649 None:intergenic
AAGTGAATAATACACAACAA+TGG 0.674679 8_1:-84630286 None:intergenic
ATAGCTGCTTGCTTCCCTGG+TGG 0.677407 8_1:+84626974 Msa1221130:CDS
CTTGCTGCTCTCAAGTACTG+GGG 0.677546 8_1:+84628205 Msa1221130:CDS
GGCTGCCGGTGAATATGGAG+AGG 0.695158 8_1:-84626823 None:intergenic
CAACGCACCTCCATCTATCG+CGG 0.695636 8_1:+84627064 Msa1221130:CDS
AGAGGAGCATCAATTTACAG+AGG 0.704191 8_1:+84628582 Msa1221130:CDS
GGGAGAGAGAAAGACCTGTG+AGG 0.704212 8_1:-84626660 None:intergenic
AAATTGAAGACTTAAAACAA+AGG 0.710279 8_1:-84630143 None:intergenic
AGGAGCATCAATTTACAGAG+GGG 0.728920 8_1:+84628584 Msa1221130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATAAATTTTTAAAAAAA+TGG - chr8_1:84629050-84629069 None:intergenic 0.0%
!!! TTTGTTTTTTGTTATAATTT+AGG + chr8_1:84628142-84628161 Msa1221130:intron 10.0%
!!! AAATTATATGAATAGTTTTA+TGG + chr8_1:84628513-84628532 Msa1221130:intron 10.0%
!! TTATTTATAGTATACTATAA+AGG + chr8_1:84628949-84628968 Msa1221130:intron 10.0%
!! TATTTATAGTATACTATAAA+GGG + chr8_1:84628950-84628969 Msa1221130:intron 10.0%
!! TATATTATTATTATGTCATT+AGG - chr8_1:84629107-84629126 None:intergenic 10.0%
!! TAAATTGAAAAAAAATGATT+AGG - chr8_1:84629199-84629218 None:intergenic 10.0%
!! TTACTATTTAAAGAAAATAT+TGG - chr8_1:84629763-84629782 None:intergenic 10.0%
!! TGAAAGATTATTTATATGTA+GGG + chr8_1:84627394-84627413 Msa1221130:intron 15.0%
!! TAGTAGTATAAATTTGAATA+AGG + chr8_1:84627471-84627490 Msa1221130:intron 15.0%
!! TTATTATGATCAAAATAGAT+CGG + chr8_1:84627821-84627840 Msa1221130:intron 15.0%
!! CATTATACAGTAAAATAAAA+GGG - chr8_1:84628286-84628305 None:intergenic 15.0%
!!! AATGTTTTACATTTTTTTGT+TGG + chr8_1:84628338-84628357 Msa1221130:intron 15.0%
!! AAACAGTTTAATAAAAAAAC+TGG - chr8_1:84628997-84629016 None:intergenic 15.0%
!! AAAATTGAAAAACACAAAAA+AGG - chr8_1:84630171-84630190 None:intergenic 15.0%
!!! TTTTTGTGTTTTTCAATTTT+TGG + chr8_1:84630171-84630190 Msa1221130:three_prime_UTR 15.0%
!!! ACTTTTTTTTTTTCATTTCC+AGG + chr8_1:84626746-84626765 Msa1221130:intron 20.0%
!! GGAATTATATATATCATTGT+CGG - chr8_1:84626939-84626958 None:intergenic 20.0%
!! GTGAAAGATTATTTATATGT+AGG + chr8_1:84627393-84627412 Msa1221130:intron 20.0%
!! TATTATGATCAAAATAGATC+GGG + chr8_1:84627822-84627841 Msa1221130:intron 20.0%
!!! TAATCATTTAGCTCATAAAA+TGG + chr8_1:84628115-84628134 Msa1221130:intron 20.0%
!!! GTTTTTTGTTATAATTTAGG+TGG + chr8_1:84628145-84628164 Msa1221130:intron 20.0%
!! GCATTATACAGTAAAATAAA+AGG - chr8_1:84628287-84628306 None:intergenic 20.0%
!!! TTTATTTTCCTTCATGTTAT+AGG + chr8_1:84628541-84628560 Msa1221130:intron 20.0%
!!! TGATAAATTCATATTCGTTT+TGG + chr8_1:84628649-84628668 Msa1221130:intron 20.0%
!!! TTGAAACAACTTTAATTTGT+TGG + chr8_1:84628730-84628749 Msa1221130:intron 20.0%
!! TAAAAAAATATTAGATGGGT+CGG - chr8_1:84628932-84628951 None:intergenic 20.0%
!! AATAAAAAAACTGGCAAAAA+TGG - chr8_1:84628988-84629007 None:intergenic 20.0%
!! AAATTGAAGACTTAAAACAA+AGG - chr8_1:84630146-84630165 None:intergenic 20.0%
!! ACATGATTTATGAGTATAAT+GGG + chr8_1:84630225-84630244 Msa1221130:three_prime_UTR 20.0%
!!! CCATAGTTTGATTTTGTTTT+TGG - chr8_1:84626477-84626496 None:intergenic 25.0%
! CCAAAAACAAAATCAAACTA+TGG + chr8_1:84626474-84626493 Msa1221130:exon 25.0%
! AGGATTAGGAAAGTTATTAT+TGG - chr8_1:84626560-84626579 None:intergenic 25.0%
! ATTATATATATCATTGTCGG+TGG - chr8_1:84626936-84626955 None:intergenic 25.0%
! AAAAAGCTAGCCAAAATATA+TGG + chr8_1:84627144-84627163 Msa1221130:intron 25.0%
!! TTGTCTATGTCCATATATTT+TGG - chr8_1:84627157-84627176 None:intergenic 25.0%
!! TGATAATTTTCAGACATAGA+TGG + chr8_1:84627277-84627296 Msa1221130:intron 25.0%
! ATTATTTATATGTAGGGACT+AGG + chr8_1:84627400-84627419 Msa1221130:intron 25.0%
! TTATTTATATGTAGGGACTA+GGG + chr8_1:84627401-84627420 Msa1221130:intron 25.0%
!! ATCTTTTGCTTCAGTTTATT+TGG + chr8_1:84627928-84627947 Msa1221130:intron 25.0%
!! TCTTTTGCTTCAGTTTATTT+GGG + chr8_1:84627929-84627948 Msa1221130:intron 25.0%
!!! ACCAAATCATCATTTTGTTT+TGG + chr8_1:84628008-84628027 Msa1221130:intron 25.0%
! GATTATTTGTACAAACTTAC+CGG - chr8_1:84628253-84628272 None:intergenic 25.0%
! ACATTTATGAAAACCGTTAA+AGG - chr8_1:84628323-84628342 None:intergenic 25.0%
!! AAGTTAAAACTCAAAGGTAT+TGG - chr8_1:84628620-84628639 None:intergenic 25.0%
! CACAAAAAGTTAAAACTCAA+AGG - chr8_1:84628626-84628645 None:intergenic 25.0%
!!! CTTTGAGTTTTAACTTTTTG+TGG + chr8_1:84628624-84628643 Msa1221130:intron 25.0%
! AATCTAATGATATACACTAG+GGG - chr8_1:84628675-84628694 None:intergenic 25.0%
! GAATCTAATGATATACACTA+GGG - chr8_1:84628676-84628695 None:intergenic 25.0%
! AGAATCTAATGATATACACT+AGG - chr8_1:84628677-84628696 None:intergenic 25.0%
! TGATGTCAATATATGGATAA+TGG - chr8_1:84628850-84628869 None:intergenic 25.0%
! TGTATAGTGATGTCAATATA+TGG - chr8_1:84628857-84628876 None:intergenic 25.0%
! TTATTTGATAAACCTCCTAT+GGG - chr8_1:84629358-84629377 None:intergenic 25.0%
! TTTCAGCAACAAAATATGAA+CGG + chr8_1:84629673-84629692 Msa1221130:CDS 25.0%
! AATAGTAGTAGTTATGAGAA+TGG + chr8_1:84629781-84629800 Msa1221130:CDS 25.0%
! GACATGATTTATGAGTATAA+TGG + chr8_1:84630224-84630243 Msa1221130:three_prime_UTR 25.0%
!!! ACAATGAAATGGATTACAAA+AGG - chr8_1:84630259-84630278 None:intergenic 25.0%
! AAGTGAATAATACACAACAA+TGG - chr8_1:84630289-84630308 None:intergenic 25.0%
ATATGAACGTGAAATATCGA+TGG - chr8_1:84626447-84626466 None:intergenic 30.0%
TATTATAGAGGAAGGATAAG+GGG - chr8_1:84626626-84626645 None:intergenic 30.0%
GTATTATAGAGGAAGGATAA+GGG - chr8_1:84626627-84626646 None:intergenic 30.0%
TTAGAGAGAAACTGAGATAT+TGG - chr8_1:84626705-84626724 None:intergenic 30.0%
!!! AAGGTATCAACGGTTTTTTT+AGG - chr8_1:84627040-84627059 None:intergenic 30.0%
AAAAAAACCGTTGATACCTT+CGG + chr8_1:84627040-84627059 Msa1221130:CDS 30.0%
! AATTTTCAGACATAGATGGA+CGG + chr8_1:84627281-84627300 Msa1221130:intron 30.0%
TCTTTAACTCCCATTTATTC+CGG - chr8_1:84627595-84627614 None:intergenic 30.0%
TTCAAAACCATTCTCAATCA+AGG + chr8_1:84627642-84627661 Msa1221130:intron 30.0%
CAGGTACAAAAGCAAAAAAA+AGG - chr8_1:84627880-84627899 None:intergenic 30.0%
!!! CTTTTTTTTGCTTTTGTACC+TGG + chr8_1:84627878-84627897 Msa1221130:intron 30.0%
GCCAAAACAAAATGATGATT+TGG - chr8_1:84628012-84628031 None:intergenic 30.0%
CATTAAAATGTACATGAAGC+AGG - chr8_1:84628042-84628061 None:intergenic 30.0%
TAATTTAGGTGGCTATGATA+AGG + chr8_1:84628156-84628175 Msa1221130:intron 30.0%
ATTGCAGCAATCAAATTTAG+AGG + chr8_1:84629265-84629284 Msa1221130:CDS 30.0%
CTTATTTGATAAACCTCCTA+TGG - chr8_1:84629359-84629378 None:intergenic 30.0%
!!! TTGAATTGGTTCGTTGATTT+TGG - chr8_1:84629416-84629435 None:intergenic 30.0%
TCTATTTCGTCAGATTGAAT+TGG - chr8_1:84629430-84629449 None:intergenic 30.0%
GAGAATATTGGACCAATAAT+CGG - chr8_1:84629521-84629540 None:intergenic 30.0%
GATTATTGGTCCAATATTCT+CGG + chr8_1:84629520-84629539 Msa1221130:CDS 30.0%
TTATCACTGTTGGTATTTGA+AGG - chr8_1:84629613-84629632 None:intergenic 30.0%
TATGAGAATGGTACTAGTTA+TGG + chr8_1:84629793-84629812 Msa1221130:CDS 30.0%
AATGGTACTAGTTATGGAAA+TGG + chr8_1:84629799-84629818 Msa1221130:CDS 30.0%
GTTATGGAAATGGAAATAAC+TGG + chr8_1:84629809-84629828 Msa1221130:CDS 30.0%
AATAGAATCAGAAGGAAACA+AGG + chr8_1:84630044-84630063 Msa1221130:three_prime_UTR 30.0%
TGTAATCCATTTCATTGTGT+TGG + chr8_1:84630261-84630280 Msa1221130:three_prime_UTR 30.0%
! TGAAGCTTAGTTGGTTTCTT+TGG - chr8_1:84626421-84626440 None:intergenic 35.0%
!! AGGTGTTGTTAGTGTTTGTA+GGG - chr8_1:84626519-84626538 None:intergenic 35.0%
GGTATTATAGAGGAAGGATA+AGG - chr8_1:84626628-84626647 None:intergenic 35.0%
! AGCTATTGTTTTCTTCAGCT+CGG - chr8_1:84626960-84626979 None:intergenic 35.0%
!! TTTTTAGGAGAAGGCTTTTG+AGG - chr8_1:84627025-84627044 None:intergenic 35.0%
!!! TCAACGGTTTTTTTAGGAGA+AGG - chr8_1:84627034-84627053 None:intergenic 35.0%
AAACATGCACTAAACATACC+TGG - chr8_1:84627095-84627114 None:intergenic 35.0%
! ATTTTCAGACATAGATGGAC+GGG + chr8_1:84627282-84627301 Msa1221130:intron 35.0%
GAGATATGAAGCACATCTAT+GGG + chr8_1:84627305-84627324 Msa1221130:CDS 35.0%
TCTGAATCCTTGATTGAGAA+TGG - chr8_1:84627652-84627671 None:intergenic 35.0%
! TGAACTTTGAAGATTCTGAC+CGG + chr8_1:84627853-84627872 Msa1221130:intron 35.0%
ACAAAAGCAAAAAAAAGGCC+CGG - chr8_1:84627875-84627894 None:intergenic 35.0%
AACTATTCACGCTCCTTTAA+CGG + chr8_1:84628307-84628326 Msa1221130:intron 35.0%
TCAGATTTCCAACTATGAGA+AGG + chr8_1:84628370-84628389 Msa1221130:intron 35.0%
ATCAATCTCCTTCTCATAGT+TGG - chr8_1:84628381-84628400 None:intergenic 35.0%
!! AATTTGTTGCTTCTCTACGA+AGG + chr8_1:84628426-84628445 Msa1221130:CDS 35.0%
CTACTCTTCCTATAACATGA+AGG - chr8_1:84628552-84628571 None:intergenic 35.0%
TCATGTTATAGGAAGAGTAG+TGG + chr8_1:84628552-84628571 Msa1221130:intron 35.0%
GTCTGCATGCTAGTAATTTA+TGG + chr8_1:84629144-84629163 Msa1221130:intron 35.0%
!! AGGGTCTTGTTTAATAGGTA+AGG - chr8_1:84629494-84629513 None:intergenic 35.0%
!! GTAGAAGGGTCTTGTTTAAT+AGG - chr8_1:84629499-84629518 None:intergenic 35.0%
ATTATGGAAGCCGAGAATAT+TGG - chr8_1:84629533-84629552 None:intergenic 35.0%
AAAATCCATGTTGAATGGTG+TGG - chr8_1:84629578-84629597 None:intergenic 35.0%
GCAGAAAAATCCATGTTGAA+TGG - chr8_1:84629583-84629602 None:intergenic 35.0%
ACTGAAAAATGCTGCATTGT+GGG - chr8_1:84629638-84629657 None:intergenic 35.0%
!! GAAAATATTGGTGTTGCGAA+TGG - chr8_1:84629751-84629770 None:intergenic 35.0%
GGAAATGGAAATAACTGGAT+TGG + chr8_1:84629814-84629833 Msa1221130:CDS 35.0%
CTGTTTCAAACGCCAATATT+TGG + chr8_1:84629868-84629887 Msa1221130:CDS 35.0%
TCAAACGCCAATATTTGGAA+TGG + chr8_1:84629873-84629892 Msa1221130:CDS 35.0%
GCTCATTCCATTCCAAATAT+TGG - chr8_1:84629883-84629902 None:intergenic 35.0%
!! GATGATGATGAAAGTGTTGA+AGG + chr8_1:84629970-84629989 Msa1221130:three_prime_UTR 35.0%
!! ATGATGAAAGTGTTGAAGGA+AGG + chr8_1:84629974-84629993 Msa1221130:three_prime_UTR 35.0%
TGCAAAGCAATAGAATCAGA+AGG + chr8_1:84630036-84630055 Msa1221130:three_prime_UTR 35.0%
ATGGTACCAACACAATGAAA+TGG - chr8_1:84630270-84630289 None:intergenic 35.0%
! GTGTTGTTAGTGTTTGTAGG+GGG - chr8_1:84626517-84626536 None:intergenic 40.0%
!! GGTGTTGTTAGTGTTTGTAG+GGG - chr8_1:84626518-84626537 None:intergenic 40.0%
!! GAGGTGTTGTTAGTGTTTGT+AGG - chr8_1:84626520-84626539 None:intergenic 40.0%
ATTGGAAAGAGAGAAAGCGA+GGG - chr8_1:84626542-84626561 None:intergenic 40.0%
TATTGGAAAGAGAGAAAGCG+AGG - chr8_1:84626543-84626562 None:intergenic 40.0%
! GATGGTGGTATTATAGAGGA+AGG - chr8_1:84626634-84626653 None:intergenic 40.0%
! GAGAGATGGTGGTATTATAG+AGG - chr8_1:84626638-84626657 None:intergenic 40.0%
ACAAACCTCTCCATATTCAC+CGG + chr8_1:84626818-84626837 Msa1221130:CDS 40.0%
GTGCATGTTTGAATTGACAG+TGG + chr8_1:84627105-84627124 Msa1221130:intron 40.0%
! TTTTCAGACATAGATGGACG+GGG + chr8_1:84627283-84627302 Msa1221130:intron 40.0%
GGAGATATGAAGCACATCTA+TGG + chr8_1:84627304-84627323 Msa1221130:CDS 40.0%
TATGGGACAATAGTTGCAGA+AGG + chr8_1:84627322-84627341 Msa1221130:CDS 40.0%
ATGGGACAATAGTTGCAGAA+GGG + chr8_1:84627323-84627342 Msa1221130:CDS 40.0%
CAAAGTAGGAAAGGAAGACA+AGG + chr8_1:84627351-84627370 Msa1221130:CDS 40.0%
ATCACAAACACTAGATCTCG+TGG - chr8_1:84627543-84627562 None:intergenic 40.0%
ACGAGATCTAGTGTTTGTGA+TGG + chr8_1:84627542-84627561 Msa1221130:intron 40.0%
CGAGATCTAGTGTTTGTGAT+GGG + chr8_1:84627543-84627562 Msa1221130:intron 40.0%
! ATCTAGTGTTTGTGATGGGA+AGG + chr8_1:84627547-84627566 Msa1221130:intron 40.0%
! TCTAGTGTTTGTGATGGGAA+GGG + chr8_1:84627548-84627567 Msa1221130:intron 40.0%
GATCAAAATAGATCGGGTGT+TGG + chr8_1:84627828-84627847 Msa1221130:intron 40.0%
! GAACTTTGAAGATTCTGACC+GGG + chr8_1:84627854-84627873 Msa1221130:intron 40.0%
!! GTGAATGATTGCTGATTCGT+GGG + chr8_1:84627900-84627919 Msa1221130:intron 40.0%
GTACAAACTTACCGGAAAGT+TGG - chr8_1:84628245-84628264 None:intergenic 40.0%
AAGAGTAGTGGTTTCTCAAG+AGG + chr8_1:84628564-84628583 Msa1221130:CDS 40.0%
!! AGAGGAGCATCAATTTACAG+AGG + chr8_1:84628582-84628601 Msa1221130:CDS 40.0%
!! GAGGAGCATCAATTTACAGA+GGG + chr8_1:84628583-84628602 Msa1221130:CDS 40.0%
!! AGGAGCATCAATTTACAGAG+GGG + chr8_1:84628584-84628603 Msa1221130:CDS 40.0%
CAATTTACAGAGGGGTAACA+AGG + chr8_1:84628592-84628611 Msa1221130:CDS 40.0%
! AAGGTAGAGGTCTTTGTTTC+CGG - chr8_1:84628805-84628824 None:intergenic 40.0%
!! AGTACATACTGAAAGTGCCA+AGG - chr8_1:84628824-84628843 None:intergenic 40.0%
GCAAATAGTTCTCTTCCCAT+AGG + chr8_1:84629340-84629359 Msa1221130:CDS 40.0%
AATAGTTCTCTTCCCATAGG+AGG + chr8_1:84629343-84629362 Msa1221130:CDS 40.0%
TCATCCATCTTCAACTTCGT+CGG + chr8_1:84629450-84629469 Msa1221130:CDS 40.0%
! AAGACCCTTCTACCGATTAT+TGG + chr8_1:84629506-84629525 Msa1221130:CDS 40.0%
TGGACCAATAATCGGTAGAA+GGG - chr8_1:84629513-84629532 None:intergenic 40.0%
TTGGACCAATAATCGGTAGA+AGG - chr8_1:84629514-84629533 None:intergenic 40.0%
TACTACCACACCATTCAACA+TGG + chr8_1:84629570-84629589 Msa1221130:CDS 40.0%
ATTGTGGGGATTATCACTGT+TGG - chr8_1:84629623-84629642 None:intergenic 40.0%
CTGAAAAATGCTGCATTGTG+GGG - chr8_1:84629637-84629656 None:intergenic 40.0%
CACTGAAAAATGCTGCATTG+TGG - chr8_1:84629639-84629658 None:intergenic 40.0%
!! CACAATGCAGCATTTTTCAG+TGG + chr8_1:84629637-84629656 Msa1221130:CDS 40.0%
CAAAATATGAACGGAAGCAG+TGG + chr8_1:84629682-84629701 Msa1221130:CDS 40.0%
TGACGACGAAGATGAAGAAT+TGG - chr8_1:84629710-84629729 None:intergenic 40.0%
! ATTGGCGTTTGAAACAGACT+TGG - chr8_1:84629865-84629884 None:intergenic 40.0%
!! TGAAAGTGTTGAAGGAAGGA+AGG + chr8_1:84629978-84629997 Msa1221130:three_prime_UTR 40.0%
GAAGATGAGTGGGAAGGATT+AGG - chr8_1:84626574-84626593 None:intergenic 45.0%
TGAAGGAGTGGAAAAGAGAG+AGG - chr8_1:84626603-84626622 None:intergenic 45.0%
GAGAGAGAGAGAGAGAGATA+GGG - chr8_1:84626683-84626702 None:intergenic 45.0%
GGTGAATATGGAGAGGTTTG+TGG - chr8_1:84626819-84626838 None:intergenic 45.0%
CTAAGAAGTCCTCGAACTTG+TGG - chr8_1:84626847-84626866 None:intergenic 45.0%
CACAAGTTCGAGGACTTCTT+AGG + chr8_1:84626845-84626864 Msa1221130:CDS 45.0%
GAAAACTGCTGAAGCTGTGT+GGG - chr8_1:84626914-84626933 None:intergenic 45.0%
TTGAGGTTCGGAGTTTGGTT+CGG - chr8_1:84627008-84627027 None:intergenic 45.0%
!!! AGGAGAAGGCTTTTGAGGTT+CGG - chr8_1:84627020-84627039 None:intergenic 45.0%
GACAATAGTTGCAGAAGGGA+AGG + chr8_1:84627327-84627346 Msa1221130:CDS 45.0%
AGGGAAGGACAAAGTAGGAA+AGG + chr8_1:84627342-84627361 Msa1221130:CDS 45.0%
ATTGTGTCTAACGTGCGCTA+TGG + chr8_1:84627494-84627513 Msa1221130:intron 45.0%
TGACCACTGACCGGAATAAA+TGG + chr8_1:84627582-84627601 Msa1221130:intron 45.0%
GACCACTGACCGGAATAAAT+GGG + chr8_1:84627583-84627602 Msa1221130:intron 45.0%
TGACAGTGTGACTGATGCAT+TGG - chr8_1:84627753-84627772 None:intergenic 45.0%
CGAATCAGCAATCATTCACC+AGG - chr8_1:84627899-84627918 None:intergenic 45.0%
!! GGTGAATGATTGCTGATTCG+TGG + chr8_1:84627899-84627918 Msa1221130:intron 45.0%
ATCTTGCTGCTCTCAAGTAC+TGG + chr8_1:84628203-84628222 Msa1221130:CDS 45.0%
TCTTGCTGCTCTCAAGTACT+GGG + chr8_1:84628204-84628223 Msa1221130:CDS 45.0%
AACGACCACTACCAACTTTC+CGG + chr8_1:84628231-84628250 Msa1221130:CDS 45.0%
!! ACTTACCGGAAAGTTGGTAG+TGG - chr8_1:84628239-84628258 None:intergenic 45.0%
GGAAACAAAGACCTCTACCT+TGG + chr8_1:84628804-84628823 Msa1221130:CDS 45.0%
!! TACTGAAAGTGCCAAGGTAG+AGG - chr8_1:84628818-84628837 None:intergenic 45.0%
GGTTTCTGATGCAGAATCTG+TGG - chr8_1:84629395-84629414 None:intergenic 45.0%
CAGCACTGCTAGGGTTATTA+TGG - chr8_1:84629549-84629568 None:intergenic 45.0%
TGGTAGTAACAGCACTGCTA+GGG - chr8_1:84629558-84629577 None:intergenic 45.0%
!! GCAGCATTTTTCAGTGGATC+AGG + chr8_1:84629643-84629662 Msa1221130:CDS 45.0%
!! GACTTGGCTTTGCATGTGTT+TGG - chr8_1:84629849-84629868 None:intergenic 45.0%
GGAATGAGCTCATGCATATC+AGG + chr8_1:84629894-84629913 Msa1221130:exon 45.0%
!! TCATGCATATCAGGTGCTGA+TGG + chr8_1:84629903-84629922 Msa1221130:three_prime_UTR 45.0%
GAAGGACAGTGTTGTAGTGA+TGG + chr8_1:84629996-84630015 Msa1221130:three_prime_UTR 45.0%
!! TAAATAAAAAAATATTAGAT+GGG - chr8_1:84628936-84628955 None:intergenic 5.0%
!! ATAAATAAAAAAATATTAGA+TGG - chr8_1:84628937-84628956 None:intergenic 5.0%
!! AAAATTTATTATTGATATAT+TGG + chr8_1:84629058-84629077 Msa1221130:intron 5.0%
!!! TCAATTTAATTTATAAATTT+AGG + chr8_1:84629211-84629230 Msa1221130:intron 5.0%
! CGATGGCGTTGAAGCTTAGT+TGG - chr8_1:84626430-84626449 None:intergenic 50.0%
GTTTGTAGGGGGTGAAGAAG+AGG - chr8_1:84626506-84626525 None:intergenic 50.0%
TGAGAGGAAGATGAGTGGGA+AGG - chr8_1:84626580-84626599 None:intergenic 50.0%
GAGGTGAGAGGAAGATGAGT+GGG - chr8_1:84626584-84626603 None:intergenic 50.0%
AGAGGTGAGAGGAAGATGAG+TGG - chr8_1:84626585-84626604 None:intergenic 50.0%
GTGGAAAAGAGAGAGGTGAG+AGG - chr8_1:84626596-84626615 None:intergenic 50.0%
AAGGATAAGGGGTGAAGGAG+TGG - chr8_1:84626615-84626634 None:intergenic 50.0%
AGAGGAAGGATAAGGGGTGA+AGG - chr8_1:84626620-84626639 None:intergenic 50.0%
GGAGAGAGAAAGACCTGTGA+GGG - chr8_1:84626662-84626681 None:intergenic 50.0%
GGAGAGAGAGAGAGAGAGAT+AGG - chr8_1:84626684-84626703 None:intergenic 50.0%
GGCTGTTCTTGTTGGTGCAA+CGG - chr8_1:84626876-84626895 None:intergenic 50.0%
GGAAAACTGCTGAAGCTGTG+TGG - chr8_1:84626915-84626934 None:intergenic 50.0%
ACAATAGCTGCTTGCTTCCC+TGG + chr8_1:84626971-84626990 Msa1221130:CDS 50.0%
!!! GGCTTTTGAGGTTCGGAGTT+TGG - chr8_1:84627013-84627032 None:intergenic 50.0%
!! GCGTTGTCCGAAGGTATCAA+CGG - chr8_1:84627050-84627069 None:intergenic 50.0%
GCAGAAGGGAAGGACAAAGT+AGG + chr8_1:84627337-84627356 Msa1221130:CDS 50.0%
TGTCTAACGTGCGCTATGGA+TGG + chr8_1:84627498-84627517 Msa1221130:intron 50.0%
GATGGTACACGGGCAAAAGT+GGG + chr8_1:84627516-84627535 Msa1221130:intron 50.0%
CAGTGAATGTGACCACTGAC+CGG + chr8_1:84627573-84627592 Msa1221130:intron 50.0%
CTCCCATTTATTCCGGTCAG+TGG - chr8_1:84627588-84627607 None:intergenic 50.0%
! CTTGCTGCTCTCAAGTACTG+GGG + chr8_1:84628205-84628224 Msa1221130:CDS 50.0%
!! GGAAAGTTGGTAGTGGTCGT+TGG - chr8_1:84628232-84628251 None:intergenic 50.0%
TGCCTATTCTTGCCTGCCAT+CGG - chr8_1:84628776-84628795 None:intergenic 50.0%
GCAAGAATAGGCAGAGTTGC+CGG + chr8_1:84628783-84628802 Msa1221130:CDS 50.0%
GTTGCCGACGAAGTTGAAGA+TGG - chr8_1:84629457-84629476 None:intergenic 50.0%
GTGGTAGTAACAGCACTGCT+AGG - chr8_1:84629559-84629578 None:intergenic 50.0%
GGAAAGAGAGAAAGCGAGGG+AGG - chr8_1:84626539-84626558 None:intergenic 55.0%
AGACCTGTGAGGGTGAGAGA+TGG - chr8_1:84626652-84626671 None:intergenic 55.0%
GGGAGAGAGAAAGACCTGTG+AGG - chr8_1:84626663-84626682 None:intergenic 55.0%
TGCCTCTGGTGAAGCTTCCA+CGG - chr8_1:84626783-84626802 None:intergenic 55.0%
GCTTCACCAGAGGCAACAGA+TGG + chr8_1:84626788-84626807 Msa1221130:CDS 55.0%
TCACCAGAGGCAACAGATGG+AGG + chr8_1:84626791-84626810 Msa1221130:CDS 55.0%
GTTCCTCCATCTGTTGCCTC+TGG - chr8_1:84626797-84626816 None:intergenic 55.0%
CTTGTGGCTGCCGGTGAATA+TGG - chr8_1:84626831-84626850 None:intergenic 55.0%
ATAGCTGCTTGCTTCCCTGG+TGG + chr8_1:84626974-84626993 Msa1221130:CDS 55.0%
!! GTTCGGAGTTTGGTTCGGTG+GGG - chr8_1:84627003-84627022 None:intergenic 55.0%
!! GGTTCGGAGTTTGGTTCGGT+GGG - chr8_1:84627004-84627023 None:intergenic 55.0%
!! AGGTTCGGAGTTTGGTTCGG+TGG - chr8_1:84627005-84627024 None:intergenic 55.0%
CAACGCACCTCCATCTATCG+CGG + chr8_1:84627064-84627083 Msa1221130:CDS 55.0%
CGTGCGCTATGGATGGTACA+CGG + chr8_1:84627505-84627524 Msa1221130:intron 55.0%
GTGCGCTATGGATGGTACAC+GGG + chr8_1:84627506-84627525 Msa1221130:intron 55.0%
GGATGGTACACGGGCAAAAG+TGG + chr8_1:84627515-84627534 Msa1221130:intron 55.0%
CGGTCAGTGGTCACATTCAC+TGG - chr8_1:84627575-84627594 None:intergenic 55.0%
CCTGTGAGGGTGAGAGATGG+TGG - chr8_1:84626649-84626668 None:intergenic 60.0%
CCACCATCTCTCACCCTCAC+AGG + chr8_1:84626646-84626665 Msa1221130:CDS 60.0%
GGCTGCCGGTGAATATGGAG+AGG - chr8_1:84626826-84626845 None:intergenic 60.0%
GTCCTCGAACTTGTGGCTGC+CGG - chr8_1:84626840-84626859 None:intergenic 60.0%
GTTGCGGCGGCTGTTCTTGT+TGG - chr8_1:84626884-84626903 None:intergenic 60.0%
CTGTGTGGGTGCACACGTTG+CGG - chr8_1:84626900-84626919 None:intergenic 60.0%
! GATGGAGGTGCGTTGTCCGA+AGG - chr8_1:84627059-84627078 None:intergenic 60.0%
GGTGACACCGCGATAGATGG+AGG - chr8_1:84627074-84627093 None:intergenic 60.0%
CCTGGTGACACCGCGATAGA+TGG - chr8_1:84627077-84627096 None:intergenic 60.0%
!! CCATCTATCGCGGTGTCACC+AGG + chr8_1:84627074-84627093 Msa1221130:CDS 60.0%
TGGTGCAGACACCACCAGCA+TGG + chr8_1:84628750-84628769 Msa1221130:intron 60.0%
GGCCGATGGCAGGCAAGAAT+AGG + chr8_1:84628771-84628790 Msa1221130:CDS 60.0%
TCCAGGAAGCAGCAACGCCG+TGG + chr8_1:84626763-84626782 Msa1221130:intron 65.0%
! TCCACGGCGTTGCTGCTTCC+TGG - chr8_1:84626767-84626786 None:intergenic 65.0%
CGCCGTGGAAGCTTCACCAG+AGG + chr8_1:84626778-84626797 Msa1221130:CDS 65.0%
CACCGGCAGCCACAAGTTCG+AGG + chr8_1:84626835-84626854 Msa1221130:CDS 65.0%
TGTGGGTGCACACGTTGCGG+CGG - chr8_1:84626897-84626916 None:intergenic 65.0%
! GTTCGGTGGGGTAGCCACCA+GGG - chr8_1:84626991-84627010 None:intergenic 65.0%
GACACCACCAGCATGGCCGA+TGG + chr8_1:84628757-84628776 Msa1221130:intron 65.0%
TTGCCTGCCATCGGCCATGC+TGG - chr8_1:84628767-84628786 None:intergenic 65.0%
!! GGTTCGGTGGGGTAGCCACC+AGG - chr8_1:84626992-84627011 None:intergenic 70.0%
CCTGCCATCGGCCATGCTGG+TGG - chr8_1:84628764-84628783 None:intergenic 70.0%
CCACCAGCATGGCCGATGGC+AGG + chr8_1:84628761-84628780 Msa1221130:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 84626387 84630365 84626387 ID=Msa1221130;Name=Msa1221130
chr8_1 mRNA 84626387 84630365 84626387 ID=Msa1221130-mRNA-1;Parent=Msa1221130;Name=Msa1221130-mRNA-1;_AED=0.14;_eAED=0.14;_QI=106|0.85|1|1|1|1|8|465|514
chr8_1 exon 84626387 84626667 84626387 ID=Msa1221130-mRNA-1:exon:30363;Parent=Msa1221130-mRNA-1
chr8_1 exon 84626768 84627095 84626768 ID=Msa1221130-mRNA-1:exon:30364;Parent=Msa1221130-mRNA-1
chr8_1 exon 84627290 84627372 84627290 ID=Msa1221130-mRNA-1:exon:30365;Parent=Msa1221130-mRNA-1
chr8_1 exon 84628164 84628252 84628164 ID=Msa1221130-mRNA-1:exon:30366;Parent=Msa1221130-mRNA-1
chr8_1 exon 84628374 84628447 84628374 ID=Msa1221130-mRNA-1:exon:30367;Parent=Msa1221130-mRNA-1
chr8_1 exon 84628563 84628613 84628563 ID=Msa1221130-mRNA-1:exon:30368;Parent=Msa1221130-mRNA-1
chr8_1 exon 84628758 84628834 84628758 ID=Msa1221130-mRNA-1:exon:30369;Parent=Msa1221130-mRNA-1
chr8_1 exon 84629233 84630365 84629233 ID=Msa1221130-mRNA-1:exon:30370;Parent=Msa1221130-mRNA-1
chr8_1 five_prime_UTR 84626387 84626492 84626387 ID=Msa1221130-mRNA-1:five_prime_utr;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84626493 84626667 84626493 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84626768 84627095 84626768 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84627290 84627372 84627290 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84628164 84628252 84628164 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84628374 84628447 84628374 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84628563 84628613 84628563 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84628758 84628834 84628758 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 CDS 84629233 84629900 84629233 ID=Msa1221130-mRNA-1:cds;Parent=Msa1221130-mRNA-1
chr8_1 three_prime_UTR 84629901 84630365 84629901 ID=Msa1221130-mRNA-1:three_prime_utr;Parent=Msa1221130-mRNA-1
Gene Sequence

>Msa1221130

ATGGACAAATCCTCTTCTTCACCCCCTACAAACACTAACAACACCTCCCTCGCTTTCTCTCTTTCCAATAATAACTTTCCTAATCCTTCCCACTCATCTTCCTCTCACCTCTCTCTTTTCCACTCCTTCACCCCTTATCCTTCCTCTATAATACCACCATCTCTCACCCTCACAGGAAGCAGCAACGCCGTGGAAGCTTCACCAGAGGCAACAGATGGAGGAACCACAAACCTCTCCATATTCACCGGCAGCCACAAGTTCGAGGACTTCTTAGGAAGCTCCGTTGCACCAACAAGAACAGCCGCCGCAACGTGTGCACCCACACAGCTTCAGCAGTTTTCCACCGACAATGATATATATAATTCCGAGCTGAAGAAAACAATAGCTGCTTGCTTCCCTGGTGGCTACCCCACCGAACCAAACTCCGAACCTCAAAAGCCTTCTCCTAAAAAAACCGTTGATACCTTCGGACAACGCACCTCCATCTATCGCGGTGTCACCAGACATAGATGGACGGGGAGATATGAAGCACATCTATGGGACAATAGTTGCAGAAGGGAAGGACAAAGTAGGAAAGGAAGACAAGGTGGCTATGATAAGGAAGAGAAAGCAGCTAGAGCTTATGATCTTGCTGCTCTCAAGTACTGGGGTCCAACGACCACTACCAACTTTCCGATTTCCAACTATGAGAAGGAGATTGATGACATGAAGAACATGACTAGACAAGAATTTGTTGCTTCTCTACGAAGGAAGAGTAGTGGTTTCTCAAGAGGAGCATCAATTTACAGAGGGGTAACAAGACACCACCAGCATGGCCGATGGCAGGCAAGAATAGGCAGAGTTGCCGGAAACAAAGACCTCTACCTTGGCACTTTCAGCACTCAAGAAGAAGCTGCAGAAGCGTATGATATTGCAGCAATCAAATTTAGAGGACTAAATGCAGTAACAAATTTTGACATGAGTCGCTACGACGTAAAGAGTATTGCAAATAGTTCTCTTCCCATAGGAGGTTTATCAAATAAGAACAACATAAATTCCACAGATTCTGCATCAGAAACCAAAATCAACGAACCAATTCAATCTGACGAAATAGATCATCCATCTTCAACTTCGTCGGCAACAACTTTAAGCTTCGCCTTACCTATTAAACAAGACCCTTCTACCGATTATTGGTCCAATATTCTCGGCTTCCATAATAACCCTAGCAGTGCTGTTACTACCACACCATTCAACATGGATTTTTCTGCTCATGCACCTTCAAATACCAACAGTGATAATCCCCACAATGCAGCATTTTTCAGTGGATCAGGTATATTTTTTCAGCAACAAAATATGAACGGAAGCAGTGGTTCCAATTCTTCATCTTCGTCGTCAGCTTCAACGAGTTCAATTCCATTCGCAACACCAATATTTTCTTTAAATAGTAATAGTAGTAGTTATGAGAATGGTACTAGTTATGGAAATGGAAATAACTGGATTGGACACACATTCCAAACACATGCAAAGCCAAGTCTGTTTCAAACGCCAATATTTGGAATGGAATGA
Protein sequence

>Msa1221130

MDKSSSSPPTNTNNTSLAFSLSNNNFPNPSHSSSSHLSLFHSFTPYPSSIIPPSLTLTGSSNAVEASPEATDGGTTNLSIFTGSHKFEDFLGSSVAPTRTAAATCAPTQLQQFSTDNDIYNSELKKTIAACFPGGYPTEPNSEPQKPSPKKTVDTFGQRTSIYRGVTRHRWTGRYEAHLWDNSCRREGQSRKGRQGGYDKEEKAARAYDLAALKYWGPTTTTNFPISNYEKEIDDMKNMTRQEFVASLRRKSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDIAAIKFRGLNAVTNFDMSRYDVKSIANSSLPIGGLSNKNNINSTDSASETKINEPIQSDEIDHPSSTSSATTLSFALPIKQDPSTDYWSNILGFHNNPSSAVTTTPFNMDFSAHAPSNTNSDNPHNAAFFSGSGIFFQQQNMNGSSGSNSSSSSSASTSSIPFATPIFSLNSNSSSYENGTSYGNGNNWIGHTFQTHAKPSLFQTPIFGME*