Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1337310 | XP_003610146.1 | 96.212 | 132 | 5 | 0 | 1 | 132 | 1 | 132 | 1.49e-86 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1337310 | G7JD05 | 96.212 | 132 | 5 | 0 | 1 | 132 | 1 | 132 | 7.09e-87 | 258 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002250 | Msa1337310 | 0.805426 | 1.342435e-49 | -8.615850e-47 |
| Msa0060070 | Msa1337310 | 0.803584 | 3.250585e-49 | -8.615850e-47 |
| Msa0743870 | Msa1337310 | 0.822925 | 1.824671e-53 | -8.615850e-47 |
| Msa0754770 | Msa1337310 | 0.806132 | 9.543759e-50 | -8.615850e-47 |
| Msa0796630 | Msa1337310 | 0.813477 | 2.509152e-51 | -8.615850e-47 |
| Msa0823540 | Msa1337310 | 0.800901 | 1.159450e-48 | -8.615850e-47 |
| Msa0829650 | Msa1337310 | 0.806143 | 9.489262e-50 | -8.615850e-47 |
| Msa0833890 | Msa1337310 | 0.801718 | 7.890534e-49 | -8.615850e-47 |
| Msa0834180 | Msa1337310 | 0.800550 | 1.367533e-48 | -8.615850e-47 |
| Msa0136630 | Msa1337310 | 0.821427 | 4.060721e-53 | -8.615850e-47 |
| Msa0160960 | Msa1337310 | 0.801990 | 6.938127e-49 | -8.615850e-47 |
| Msa0187310 | Msa1337310 | 0.813524 | 2.450969e-51 | -8.615850e-47 |
| Msa1259860 | Msa1337310 | 0.826221 | 3.056073e-54 | -8.615850e-47 |
| Msa1280170 | Msa1337310 | 0.801567 | 8.471118e-49 | -8.615850e-47 |
| Msa1337310 | Msa1360020 | 0.807899 | 4.034821e-50 | -8.615850e-47 |
| Msa1337310 | Msa1386890 | 0.815905 | 7.273881e-52 | -8.615850e-47 |
| Msa1337310 | Msa1388090 | 0.805248 | 1.463171e-49 | -8.615850e-47 |
| Msa0380600 | Msa1337310 | 0.823318 | 1.477828e-53 | -8.615850e-47 |
| Msa0382370 | Msa1337310 | 0.810853 | 9.377055e-51 | -8.615850e-47 |
| Msa0430240 | Msa1337310 | 0.821303 | 4.337004e-53 | -8.615850e-47 |
| Msa0432160 | Msa1337310 | 0.820150 | 7.985015e-53 | -8.615850e-47 |
| Msa0476680 | Msa1337310 | 0.817717 | 2.852675e-52 | -8.615850e-47 |
| Msa0314000 | Msa1337310 | 0.821624 | 3.657422e-53 | -8.615850e-47 |
| Msa1177560 | Msa1337310 | 0.811572 | 6.545391e-51 | -8.615850e-47 |
| Msa1212050 | Msa1337310 | 0.824349 | 8.470007e-54 | -8.615850e-47 |
| Msa1212130 | Msa1337310 | 0.828297 | 9.721793e-55 | -8.615850e-47 |
| Msa1214170 | Msa1337310 | 0.808345 | 3.241887e-50 | -8.615850e-47 |
| Msa0484800 | Msa1337310 | 0.830670 | 2.576230e-55 | -8.615850e-47 |
| Msa0521010 | Msa1337310 | 0.819398 | 1.185896e-52 | -8.615850e-47 |
| Msa0863990 | Msa1337310 | 0.810736 | 9.938034e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1337310 | MtrunA17_Chr4g0072541 | 96.212 | 132 | 5 | 0 | 1 | 132 | 1 | 132 | 1.37e-90 | 258 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1337310 | AT5G57510.1 | 48.800 | 125 | 58 | 5 | 6 | 127 | 3 | 124 | 1.87e-26 | 96.3 |
Find 19 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGGGAACAAAAGAGTTC+TGG | 0.299960 | tig0001526:-20802 | Msa1337310:CDS |
| CATTGCAGCTACGTTATTGC+AGG | 0.354632 | tig0001526:-20819 | Msa1337310:CDS |
| CTCCATCTCCTTGTCTTCAA+AGG | 0.416512 | tig0001526:-20939 | Msa1337310:CDS |
| GTTCCATGTGTGGTTGAGTA+TGG | 0.434971 | tig0001526:+21074 | None:intergenic |
| CCTACGAACGGCGGTGCAAA+AGG | 0.478843 | tig0001526:-21118 | Msa1337310:CDS |
| CTTATTGTCCTTTGAAGACA+AGG | 0.489660 | tig0001526:+20931 | None:intergenic |
| CTAAGTTTCAACGATCGTCC+TGG | 0.511331 | tig0001526:-21009 | Msa1337310:CDS |
| GTCCTTTGAAGACAAGGAGA+TGG | 0.521798 | tig0001526:+20937 | None:intergenic |
| GAAGAATACATCATCAATCC+AGG | 0.534017 | tig0001526:+20991 | None:intergenic |
| TTCAGATGAAACTGATTCAG+AGG | 0.544944 | tig0001526:-20971 | Msa1337310:CDS |
| CCACCATACTCAACCACACA+TGG | 0.550819 | tig0001526:-21077 | Msa1337310:CDS |
| TCAGATGAAACTGATTCAGA+GGG | 0.570568 | tig0001526:-20970 | Msa1337310:CDS |
| CCATGTGTGGTTGAGTATGG+TGG | 0.585997 | tig0001526:+21077 | None:intergenic |
| TTTGCACCGCCGTTCGTAGG+CGG | 0.592132 | tig0001526:+21121 | None:intergenic |
| GGATGACATTGATAAGAGAT+CGG | 0.600101 | tig0001526:-20893 | Msa1337310:CDS |
| TCAATGTCATCCTCATCTGA+AGG | 0.601031 | tig0001526:+20904 | None:intergenic |
| ATTGCAGCTACGTTATTGCA+GGG | 0.621027 | tig0001526:-20818 | Msa1337310:CDS |
| TCTACTAAGCCGCCTACGAA+CGG | 0.672450 | tig0001526:-21130 | Msa1337310:CDS |
| ACTAAGCCGCCTACGAACGG+CGG | 0.714763 | tig0001526:-21127 | Msa1337310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAAGTTTTCCTTCAGATG+AGG | - | tig0001526:21034-21053 | Msa1337310:CDS | 30.0% |
| GAAGAATACATCATCAATCC+AGG | + | tig0001526:20960-20979 | None:intergenic | 35.0% | |
| ! | TTCAGATGAAACTGATTCAG+AGG | - | tig0001526:20977-20996 | Msa1337310:CDS | 35.0% |
| ! | TCAGATGAAACTGATTCAGA+GGG | - | tig0001526:20978-20997 | Msa1337310:CDS | 35.0% |
| ! | CTTATTGTCCTTTGAAGACA+AGG | + | tig0001526:21020-21039 | None:intergenic | 35.0% |
| GGATGACATTGATAAGAGAT+CGG | - | tig0001526:21055-21074 | Msa1337310:CDS | 35.0% | |
| TCAATGTCATCCTCATCTGA+AGG | + | tig0001526:21047-21066 | None:intergenic | 40.0% | |
| ATTGCAGCTACGTTATTGCA+GGG | - | tig0001526:21130-21149 | Msa1337310:CDS | 40.0% | |
| GTTCCATGTGTGGTTGAGTA+TGG | + | tig0001526:20877-20896 | None:intergenic | 45.0% | |
| ! | TTTTTGCAGCGTTCCATGTG+TGG | + | tig0001526:20887-20906 | None:intergenic | 45.0% |
| CTAAGTTTCAACGATCGTCC+TGG | - | tig0001526:20939-20958 | Msa1337310:CDS | 45.0% | |
| CTCCATCTCCTTGTCTTCAA+AGG | - | tig0001526:21009-21028 | Msa1337310:CDS | 45.0% | |
| ! | GTCCTTTGAAGACAAGGAGA+TGG | + | tig0001526:21014-21033 | None:intergenic | 45.0% |
| CATTGCAGCTACGTTATTGC+AGG | - | tig0001526:21129-21148 | Msa1337310:CDS | 45.0% | |
| TGCAGGGAACAAAAGAGTTC+TGG | - | tig0001526:21146-21165 | Msa1337310:CDS | 45.0% | |
| TCTACTAAGCCGCCTACGAA+CGG | - | tig0001526:20818-20837 | Msa1337310:CDS | 50.0% | |
| CCATGTGTGGTTGAGTATGG+TGG | + | tig0001526:20874-20893 | None:intergenic | 50.0% | |
| CCACCATACTCAACCACACA+TGG | - | tig0001526:20871-20890 | Msa1337310:CDS | 50.0% | |
| ACTAAGCCGCCTACGAACGG+CGG | - | tig0001526:20821-20840 | Msa1337310:CDS | 60.0% | |
| TTTGCACCGCCGTTCGTAGG+CGG | + | tig0001526:20830-20849 | None:intergenic | 60.0% | |
| ! | CCTTTTGCACCGCCGTTCGT+AGG | + | tig0001526:20833-20852 | None:intergenic | 60.0% |
| CCTACGAACGGCGGTGCAAA+AGG | - | tig0001526:20830-20849 | Msa1337310:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0001526 | gene | 20786 | 21184 | 20786 | ID=Msa1337310;Name=Msa1337310 |
| tig0001526 | mRNA | 20786 | 21184 | 20786 | ID=Msa1337310-mRNA-1;Parent=Msa1337310;Name=Msa1337310-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|132 |
| tig0001526 | exon | 20786 | 21184 | 20786 | ID=Msa1337310-mRNA-1:exon:1103;Parent=Msa1337310-mRNA-1 |
| tig0001526 | CDS | 20786 | 21184 | 20786 | ID=Msa1337310-mRNA-1:cds;Parent=Msa1337310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
