AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1351900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351900 MtrunA17_Chr7g0271411 90.034 291 24 2 7 297 20 305 4.31e-158 442
Msa1351900 MtrunA17_Chr1g0193131 56.388 227 64 10 29 227 279 498 1.85e-49 173
Msa1351900 MtrunA17_Chr5g0443331 57.037 135 55 2 124 258 86 217 1.21e-38 137
Msa1351900 MtrunA17_Chr3g0096451 63.551 107 36 1 126 232 93 196 1.89e-38 136
Msa1351900 MtrunA17_Chr2g0304061 62.069 87 32 1 148 234 158 243 5.39e-32 121
Msa1351900 MtrunA17_Chr8g0381081 46.903 113 52 3 126 233 34 143 4.90e-28 106
Msa1351900 MtrunA17_Chr1g0177171 40.667 150 80 4 89 233 347 492 9.80e-27 109
Msa1351900 MtrunA17_Chr4g0040911 49.153 118 47 3 148 262 37 144 1.82e-26 102
Msa1351900 MtrunA17_Chr8g0378361 51.042 96 45 2 146 240 28 122 7.71e-26 101
Msa1351900 MtrunA17_Chr8g0378621 39.610 154 75 4 141 289 357 497 8.52e-26 106
Msa1351900 MtrunA17_Chr5g0401301 41.667 144 72 4 92 231 279 414 1.33e-25 105
Msa1351900 MtrunA17_Chr4g0075031 51.613 93 44 1 141 232 375 467 4.16e-25 104
Msa1351900 MtrunA17_Chr8g0378371 55.172 87 36 2 147 233 32 115 3.85e-24 94.4
Msa1351900 MtrunA17_Chr3g0121271 39.695 131 68 3 135 264 349 469 6.94e-23 98.2
Msa1351900 MtrunA17_Chr8g0380231 59.155 71 29 0 155 225 24 94 3.78e-22 89.0
Msa1351900 MtrunA17_Chr1g0154991 47.706 109 46 5 134 239 8 108 4.02e-22 89.0
Msa1351900 MtrunA17_Chr8g0378381 50.562 89 38 3 147 232 31 116 5.73e-22 89.0
Msa1351900 MtrunA17_Chr5g0434591 42.056 107 61 1 141 246 353 459 1.41e-21 94.4
Msa1351900 MtrunA17_Chr5g0447651 49.412 85 42 1 148 231 30 114 1.77e-21 87.4
Msa1351900 MtrunA17_Chr5g0439251 47.253 91 47 1 141 230 375 465 3.05e-21 93.2
Msa1351900 MtrunA17_Chr3g0110371 43.299 97 54 1 141 236 354 450 4.96e-21 92.8
Msa1351900 MtrunA17_Chr4g0034901 43.860 114 54 3 130 235 352 463 2.12e-20 90.9
Msa1351900 MtrunA17_Chr4g0043511 47.778 90 45 2 147 235 419 507 2.78e-20 90.9
Msa1351900 MtrunA17_Chr3g0120401 45.977 87 45 2 148 233 157 242 2.82e-20 87.8
Msa1351900 MtrunA17_Chr5g0419121 47.368 95 46 3 143 234 17 110 4.66e-20 87.4
Msa1351900 MtrunA17_Chr8g0378401 50.602 83 35 3 147 226 31 110 1.82e-19 82.0
Msa1351900 MtrunA17_Chr1g0161011 43.269 104 53 2 147 249 360 458 2.17e-19 88.2
Msa1351900 MtrunA17_Chr4g0076131 41.379 87 50 1 147 232 27 113 4.54e-19 80.9
Msa1351900 MtrunA17_Chr6g0487261 40.541 111 61 4 123 231 347 454 2.16e-18 85.1
Msa1351900 MtrunA17_Chr4g0043151 47.253 91 45 3 148 236 21 110 2.84e-18 81.3
Msa1351900 MtrunA17_Chr1g0213651 44.706 85 45 2 148 231 276 359 3.07e-18 84.0
Msa1351900 MtrunA17_Chr4g0076141 43.210 81 45 1 147 226 12 92 4.92e-18 77.8
Msa1351900 MtrunA17_Chr4g0061401 44.944 89 46 3 144 231 20 106 5.31e-18 80.9
Msa1351900 MtrunA17_Chr8g0389741 33.099 142 84 3 123 258 348 484 1.59e-17 82.4
Msa1351900 MtrunA17_Chr2g0313891 44.186 86 45 2 147 231 29 112 1.39e-16 74.3
Msa1351900 MtrunA17_Chr3g0144531 40.385 104 56 3 131 231 354 454 1.94e-16 79.3
Msa1351900 MtrunA17_Chr7g0234821 38.983 118 67 3 120 235 348 462 2.74e-16 79.0
Msa1351900 MtrunA17_Chr8g0339191 36.082 97 61 1 139 234 365 461 3.84e-16 78.2
Msa1351900 MtrunA17_Chr2g0306311 40.816 98 53 4 147 242 398 492 8.35e-14 71.6
Msa1351900 MtrunA17_Chr7g0223961 41.379 87 45 3 146 229 363 446 1.04e-13 71.2
Msa1351900 MtrunA17_Chr3g0129341 35.714 112 62 5 128 232 348 456 4.75e-13 69.3
Msa1351900 MtrunA17_Chr4g0062771 34.483 116 71 4 147 260 373 485 8.30e-13 68.6
Msa1351900 MtrunA17_Chr4g0046311 36.905 84 52 1 144 226 369 452 8.49e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351900 AT1G09460.1 59.375 128 48 2 108 235 99 222 1.39e-37 136
Msa1351900 AT2G30933.1 61.798 89 34 0 147 235 81 169 4.14e-34 124
Msa1351900 AT1G29380.3 58.889 90 37 0 147 236 147 236 7.09e-33 122
Msa1351900 AT1G29380.1 58.824 85 35 0 147 231 147 231 1.57e-31 120
Msa1351900 AT2G30933.2 62.025 79 30 0 147 225 81 159 1.02e-30 113
Msa1351900 AT2G30933.3 62.025 79 30 0 147 225 81 159 1.02e-30 113
Msa1351900 AT1G29380.4 58.750 80 33 0 147 226 147 226 2.75e-29 111
Msa1351900 AT1G29380.2 58.750 80 33 0 147 226 147 226 2.75e-29 111
Msa1351900 AT1G11820.2 46.032 126 64 2 135 256 368 493 1.45e-27 112
Msa1351900 AT4G05430.3 45.133 113 57 2 128 235 24 136 2.95e-27 104
Msa1351900 AT4G05430.1 50.000 92 45 1 144 234 19 110 4.48e-27 103
Msa1351900 AT3G58100.1 46.218 119 62 2 148 265 41 158 1.80e-26 103
Msa1351900 AT3G58100.2 46.218 119 62 2 148 265 117 234 4.51e-26 103
Msa1351900 AT4G05430.2 47.115 104 50 2 128 226 24 127 2.07e-25 98.6
Msa1351900 AT2G01630.1 44.340 106 58 1 141 245 353 458 3.59e-25 105
Msa1351900 AT1G66250.1 37.415 147 81 4 141 284 361 499 6.19e-25 104
Msa1351900 AT4G26830.1 43.590 117 53 2 118 233 349 453 6.65e-25 103
Msa1351900 AT4G26830.2 43.590 117 53 2 118 233 353 457 6.92e-25 103
Msa1351900 AT5G55180.1 52.222 90 42 1 145 233 369 458 1.41e-24 103
Msa1351900 AT2G05790.1 33.198 247 141 9 3 233 232 470 1.75e-24 103
Msa1351900 AT5G35740.1 47.368 95 46 3 141 232 23 116 6.09e-24 94.4
Msa1351900 AT4G13600.1 43.182 132 55 4 148 261 61 190 2.72e-23 95.9
Msa1351900 AT1G11820.3 51.087 92 44 1 135 225 368 459 1.71e-22 97.4
Msa1351900 AT1G18650.1 46.237 93 48 2 144 235 17 108 1.98e-22 92.4
Msa1351900 AT1G18650.2 46.237 93 48 2 144 235 17 108 3.29e-22 90.1
Msa1351900 AT5G55180.2 53.012 83 38 1 145 226 369 451 9.96e-22 95.1
Msa1351900 AT5G67460.1 38.571 140 70 5 100 231 246 377 1.93e-21 93.6
Msa1351900 AT4G34480.3 47.674 86 43 2 148 232 253 337 3.14e-21 93.2
Msa1351900 AT4G34480.1 47.674 86 43 2 148 232 364 448 4.03e-21 93.6
Msa1351900 AT2G01630.3 46.512 86 45 1 141 225 353 438 2.13e-20 91.3
Msa1351900 AT5G67460.2 38.806 134 66 5 100 225 246 371 6.31e-20 89.4
Msa1351900 AT5G24318.3 41.121 107 57 3 128 233 217 318 6.52e-20 88.6
Msa1351900 AT2G16230.1 44.554 101 51 3 148 247 366 462 9.65e-20 89.4
Msa1351900 AT5G24318.1 41.121 107 57 3 128 233 356 457 1.01e-19 89.4
Msa1351900 AT3G55430.1 42.553 94 52 2 139 231 357 449 1.20e-19 89.0
Msa1351900 AT5G63240.1 40.179 112 60 5 126 233 20 128 8.28e-19 81.3
Msa1351900 AT5G63250.1 40.816 98 53 3 140 233 34 130 8.40e-19 81.3
Msa1351900 AT1G66870.1 38.298 94 55 2 140 231 18 110 2.81e-18 79.3
Msa1351900 AT1G66870.2 38.298 94 55 2 140 231 45 137 3.19e-18 79.7
Msa1351900 AT1G79480.2 44.706 85 45 2 148 231 310 393 4.06e-18 84.3
Msa1351900 AT1G79480.1 44.706 85 45 2 148 231 311 394 4.21e-18 84.3
Msa1351900 AT2G04910.2 40.860 93 54 1 141 232 8 100 4.37e-18 78.6
Msa1351900 AT5G24318.2 42.424 99 51 3 128 225 356 449 6.43e-18 84.0
Msa1351900 AT5G63225.1 41.860 86 48 2 147 231 24 108 1.20e-17 77.4
Msa1351900 AT1G66852.1 34.783 92 60 0 140 231 18 109 1.43e-17 77.0
Msa1351900 AT2G43660.2 41.758 91 52 1 146 235 33 123 2.79e-17 76.6
Msa1351900 AT2G43660.1 41.758 91 52 1 146 235 32 122 3.08e-17 76.6
Msa1351900 AT5G56590.1 31.250 144 83 3 148 290 369 497 3.10e-17 82.0
Msa1351900 AT4G16165.1 38.710 93 56 1 140 231 18 110 3.67e-17 76.3
Msa1351900 AT2G39640.1 35.246 122 68 3 123 234 426 546 4.85e-17 81.6
Msa1351900 AT2G39640.1 40.909 88 50 2 143 229 354 440 1.06e-14 74.7
Msa1351900 AT2G43670.1 40.426 94 53 2 140 232 27 118 8.10e-17 75.5
Msa1351900 AT1G69295.1 45.349 86 44 3 144 228 17 100 8.40e-17 78.2
Msa1351900 AT4G09465.1 40.217 92 53 2 143 233 26 116 1.06e-16 75.1
Msa1351900 AT4G09464.1 40.217 92 53 2 143 233 26 116 1.14e-16 75.1
Msa1351900 AT4G09467.1 40.217 92 53 2 143 233 26 116 1.14e-16 75.1
Msa1351900 AT3G23770.1 39.048 105 60 2 132 235 374 475 1.39e-16 80.1
Msa1351900 AT4G09462.1 39.000 100 59 2 135 233 16 114 1.42e-16 74.7
Msa1351900 AT5G08000.2 48.387 93 46 2 141 232 33 124 1.46e-16 77.4
Msa1351900 AT1G66855.1 39.535 86 51 1 147 231 25 110 1.50e-16 74.3
Msa1351900 AT1G69295.2 45.349 86 44 3 144 228 13 96 2.85e-16 76.6
Msa1351900 AT2G04910.1 41.379 87 50 1 141 226 8 94 3.23e-16 73.9
Msa1351900 AT4G09466.1 40.217 92 53 2 143 233 26 116 3.31e-16 73.6
Msa1351900 AT4G09090.1 42.529 87 48 2 148 233 31 116 5.24e-16 73.2
Msa1351900 AT5G61130.1 43.333 90 49 2 144 232 17 105 5.59e-16 75.5
Msa1351900 AT3G13560.4 40.625 96 56 1 148 242 362 457 1.00e-15 77.8
Msa1351900 AT3G13560.1 40.625 96 56 1 148 242 362 457 1.00e-15 77.8
Msa1351900 AT3G13560.2 40.625 96 56 1 148 242 362 457 1.00e-15 77.8
Msa1351900 AT3G13560.5 40.625 96 56 1 148 242 362 457 1.00e-15 77.8
Msa1351900 AT3G13560.3 40.625 96 56 1 148 242 362 457 1.00e-15 77.8
Msa1351900 AT4G29360.2 38.636 88 53 1 148 234 401 488 1.18e-15 77.4
Msa1351900 AT4G29360.1 38.636 88 53 1 148 234 391 478 1.24e-15 77.4
Msa1351900 AT5G08000.1 48.276 87 43 2 147 232 20 105 1.40e-15 73.9
Msa1351900 AT5G53600.1 39.785 93 53 2 140 231 21 111 1.54e-15 71.6
Msa1351900 AT1G26450.1 45.455 77 39 3 144 219 17 91 8.30e-15 72.0
Msa1351900 AT2G42930.1 39.241 79 47 1 148 226 40 117 8.44e-15 70.5
Msa1351900 AT5G63230.1 37.209 86 52 2 147 231 25 109 9.78e-15 69.7
Msa1351900 AT1G78520.1 42.529 87 47 2 147 232 31 115 9.96e-15 69.7
Msa1351900 AT4G14080.1 35.897 117 67 3 124 235 364 477 1.21e-14 74.3
Msa1351900 AT2G03505.4 36.170 94 56 3 144 234 17 109 3.22e-14 69.7
Msa1351900 AT2G03505.1 36.170 94 56 3 144 234 17 109 3.22e-14 69.7
Msa1351900 AT2G03505.2 36.170 94 56 3 144 234 17 109 3.22e-14 69.7
Msa1351900 AT2G03505.3 36.458 96 57 3 144 236 17 111 3.44e-14 68.2
Msa1351900 AT3G07320.1 39.785 93 55 1 144 235 367 459 8.94e-14 71.6
Msa1351900 AT5G53610.1 41.176 85 47 2 148 231 28 110 1.19e-13 66.6
Msa1351900 AT1G13830.2 43.373 83 40 4 145 225 32 109 5.92e-13 67.0
Msa1351900 AT1G13830.1 43.750 80 38 4 148 225 21 95 1.11e-12 66.2
Msa1351900 AT3G04010.1 40.860 93 49 4 147 236 373 462 4.51e-12 66.6
Msa1351900 AT4G17180.1 38.372 86 49 3 147 231 358 440 6.42e-12 66.2
Msa1351900 AT5G58090.1 41.573 89 46 4 147 232 361 446 8.56e-12 65.9
Msa1351900 AT2G29735.1 35.870 92 55 3 143 231 26 116 8.01e-11 58.9

Find 116 sgRNAs with CRISPR-Local

Find 344 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATGGTGTTGGTAAAATT+TGG 0.173757 tig0012395:+2236 Msa1351900:three_prime_UTR
AATGGAAAATGCATGCATTT+AGG 0.226405 tig0012395:-2385 None:intergenic
ACATCATCTCCAGTAGGTTT+TGG 0.266537 tig0012395:+1684 Msa1351900:CDS
GCTGGATTTGTTGGTGATAA+TGG 0.267994 tig0012395:-280 None:intergenic
TAGAAAGACAGTAGAGTAGT+AGG 0.271212 tig0012395:+1877 Msa1351900:three_prime_UTR
TTTGCTAAGCTGCATGGTTC+TGG 0.314068 tig0012395:+1755 Msa1351900:CDS
AGTCCTCCATCTTCTTCTTC+AGG 0.320635 tig0012395:+436 Msa1351900:CDS
TTCAGGAACTGGAGTAGTTT+AGG 0.325211 tig0012395:+2010 Msa1351900:three_prime_UTR
TGCATCTTCCCATCAGCTTC+AGG 0.344512 tig0012395:+806 Msa1351900:CDS
TTGTAACAGATCACAAGATT+TGG 0.357056 tig0012395:+125 Msa1351900:CDS
TAGAGCATGGAAGCTAACTA+TGG 0.359132 tig0012395:-1813 None:intergenic
AGTGTAGATTGTGGTGTTCC+AGG 0.362624 tig0012395:-481 None:intergenic
TCTTGGCTATAATCAGATTC+AGG 0.369696 tig0012395:+1993 Msa1351900:three_prime_UTR
GCTTACAAATCCAACATCTT+AGG 0.371314 tig0012395:+2293 Msa1351900:three_prime_UTR
CCACCACCATCACCATTATC+AGG 0.376324 tig0012395:+367 Msa1351900:CDS
GTTCCTGTTCCTCCTGATAA+TGG 0.379708 tig0012395:-379 None:intergenic
GGTGAATTTGCTGGATTTGT+TGG 0.382630 tig0012395:-289 None:intergenic
CATGGTTCTGGTGACATTAT+TGG 0.382811 tig0012395:+1767 Msa1351900:CDS
GCTGCAACAAGCTGTGACTT+TGG 0.388632 tig0012395:+643 Msa1351900:CDS
TTGGATTCCTCTCATTCAAC+TGG 0.389358 tig0012395:+1723 Msa1351900:CDS
GGCACAGGCACAGGCACATC+CGG 0.389361 tig0012395:+845 Msa1351900:CDS
GGAACTGTTGGTGGTGTAAC+AGG 0.393025 tig0012395:-316 None:intergenic
AGTGTGTGCATTGTTTGTTT+TGG 0.397338 tig0012395:+2047 Msa1351900:three_prime_UTR
CATATGTAAATCAACAACTA+TGG 0.412229 tig0012395:+2220 Msa1351900:three_prime_UTR
GGAGGAGCTGGCGCAGGCAC+AGG 0.414477 tig0012395:+830 Msa1351900:CDS
CTTCTTCTTCAGGAGGTAGC+TGG 0.414888 tig0012395:+446 Msa1351900:CDS
GCTGGCGCAGGCACAGGCAC+AGG 0.415709 tig0012395:+836 Msa1351900:CDS
GACTATGCTTGTGGAACAAA+AGG 0.417962 tig0012395:+517 Msa1351900:CDS
CTATAATCAGATTCAGGAAC+TGG 0.422203 tig0012395:+1999 Msa1351900:three_prime_UTR
TTCACTGGTGTTGTTCCTCC+TGG 0.427069 tig0012395:-337 None:intergenic
TGACAGTGACAGCGTTGAAA+TGG 0.428428 tig0012395:-2403 None:intergenic
GTTACACCACCAACAGTTCC+AGG 0.439114 tig0012395:+319 Msa1351900:CDS
ACAGGTACTGGTGAATTTGC+TGG 0.439151 tig0012395:-298 None:intergenic
AGAAATAACTGAGGACCTCT+TGG 0.442274 tig0012395:+1976 Msa1351900:three_prime_UTR
AAGTCACAGCTTGTTGCAGC+AGG 0.460540 tig0012395:-640 None:intergenic
GTTGTTCCTCCTGGAACTGT+TGG 0.460619 tig0012395:-328 None:intergenic
TCAGCTTCAGGAGGAGGAGC+TGG 0.463929 tig0012395:+818 Msa1351900:CDS
GGTGTTGCACCTGGATTTGT+TGG 0.466876 tig0012395:-223 None:intergenic
ACAGCTGGTGGAGTAGTAGA+AGG 0.473175 tig0012395:-256 None:intergenic
AAAATAAGTCATGTGGGTAC+TGG 0.473219 tig0012395:+2101 Msa1351900:three_prime_UTR
AGAAAGACAGTAGAGTAGTA+GGG 0.474208 tig0012395:+1878 Msa1351900:three_prime_UTR
CAGTGATGATTGTTGGTGAT+GGG 0.480237 tig0012395:-168 None:intergenic
TCTGAATTTGCGAAAATGAA+AGG 0.480451 tig0012395:-2352 None:intergenic
AGCTGGTGTGTGGCTAAACC+TGG 0.480971 tig0012395:+463 Msa1351900:CDS
GTTCCTCCTGGAACTGTTGG+TGG 0.482015 tig0012395:-325 None:intergenic
TGGGCCGCAAAGCTCTCCAT+TGG 0.483468 tig0012395:+1704 Msa1351900:CDS
TCACCATTATCAGGAGGAAC+AGG 0.487525 tig0012395:+376 Msa1351900:CDS
TTCCCATCAGCTTCAGGAGG+AGG 0.492744 tig0012395:+812 Msa1351900:CDS
TCAGGAGGAGGAGCTGGCGC+AGG 0.502820 tig0012395:+824 Msa1351900:CDS
CTTTGCGGCCCAAAACCTAC+TGG 0.504420 tig0012395:-1693 None:intergenic
CAACAAGCTGTGACTTTGGA+GGG 0.506706 tig0012395:+647 Msa1351900:CDS
ATCTACACTTCAAACAGCAT+TGG 0.509235 tig0012395:+495 Msa1351900:CDS
TCATTGCACTAACTAGTAGA+TGG 0.513999 tig0012395:+2135 Msa1351900:three_prime_UTR
AGAAATAGTTGAAAATGCAT+TGG 0.517953 tig0012395:-50 None:intergenic
AAGCTTGAGTAAACAGATCA+AGG 0.518696 tig0012395:-1932 None:intergenic
GATGGAGGACTTGTGCTTGA+AGG 0.519243 tig0012395:-424 None:intergenic
GTAAAATCGTAGTGGTCTGA+TGG 0.520816 tig0012395:-2317 None:intergenic
GAGACCTTTGCTAAGCTGCA+TGG 0.522316 tig0012395:+1749 Msa1351900:CDS
GAAGGAACAGTGATGATTGT+TGG 0.527796 tig0012395:-175 None:intergenic
GCAACAAGCTGTGACTTTGG+AGG 0.530607 tig0012395:+646 Msa1351900:CDS
GAAGGTGGAGGATTTGTGAC+AGG 0.530909 tig0012395:-406 None:intergenic
TGTTATACAATTCTACAAGC+AGG 0.531967 tig0012395:+1852 Msa1351900:three_prime_UTR
TAAATCAACAACTATGGTGT+TGG 0.532644 tig0012395:+2226 Msa1351900:three_prime_UTR
GTTGGAGAAACTGTTGTGAC+AGG 0.533113 tig0012395:-205 None:intergenic
GAATCCAATGGAGAGCTTTG+CGG 0.535900 tig0012395:-1708 None:intergenic
CCTGATAATGGTGATGGTGG+TGG 0.538366 tig0012395:-367 None:intergenic
CTCCTCCTCCTGAAGCTGAT+GGG 0.539809 tig0012395:-814 None:intergenic
TGAAGCTGATGGGAAGATGC+AGG 0.543799 tig0012395:-804 None:intergenic
ATTCAAGAAAATAAGTCATG+TGG 0.550923 tig0012395:+2094 Msa1351900:three_prime_UTR
CTACCTCCTGAAGAAGAAGA+TGG 0.552545 tig0012395:-442 None:intergenic
ACAGTGATGATTGTTGGTGA+TGG 0.553424 tig0012395:-169 None:intergenic
GTTCCTCCTGATAATGGTGA+TGG 0.553903 tig0012395:-373 None:intergenic
GCTCCTCCTCCTGAAGCTGA+TGG 0.558816 tig0012395:-815 None:intergenic
GGACTTGTGCTTGAAGGTGG+AGG 0.559600 tig0012395:-418 None:intergenic
GTTGGTGATAATGGAACAGC+TGG 0.559840 tig0012395:-271 None:intergenic
GTAGTAGAAGGAACTGTGAC+AGG 0.561662 tig0012395:-244 None:intergenic
CAGGCACAGGCACATCCGGC+GGG 0.564968 tig0012395:+849 Msa1351900:CDS
AGAGTAGTAGGGAGAGTTTG+TGG 0.566599 tig0012395:+1889 Msa1351900:three_prime_UTR
ACAGTTTCTCCAACAAATCC+AGG 0.567466 tig0012395:+214 Msa1351900:CDS
TTCAGGAGGTAGCTGGTGTG+TGG 0.575773 tig0012395:+453 Msa1351900:CDS
TTCAAGAAAATAAGTCATGT+GGG 0.579136 tig0012395:+2095 Msa1351900:three_prime_UTR
TACAGCGAGGAGATAGAGCA+TGG 0.583522 tig0012395:-1826 None:intergenic
GTTGGTGGTGTAACAGGTAC+TGG 0.587227 tig0012395:-310 None:intergenic
ACTGTGACAGGTGTTGCACC+TGG 0.594459 tig0012395:-232 None:intergenic
GTTGTGACAGGATTGGTTGA+AGG 0.600451 tig0012395:-193 None:intergenic
ACAGCATTGGACTATGCTTG+TGG 0.604056 tig0012395:+508 Msa1351900:CDS
CCTCCATCTTCTTCTTCAGG+AGG 0.605110 tig0012395:+439 Msa1351900:CDS
GTCTGATGGACCTAAGATGT+TGG 0.605429 tig0012395:-2303 None:intergenic
AACAAGCTGTGACTTTGGAG+GGG 0.609446 tig0012395:+648 Msa1351900:CDS
AGAGCATGGAAGCTAACTAT+GGG 0.609588 tig0012395:-1812 None:intergenic
AGAAACTGTTGTGACAGGAT+TGG 0.611206 tig0012395:-200 None:intergenic
CCTCCTGATAATGGTGATGG+TGG 0.614026 tig0012395:-370 None:intergenic
TTATTGGTCAGTGGAAGACT+CGG 0.614232 tig0012395:+1783 Msa1351900:CDS
TGTTGAATGCAAAAGAAGCA+TGG 0.614920 tig0012395:-600 None:intergenic
GATTGTAACAGCTACCACCT+TGG 0.617114 tig0012395:-561 None:intergenic
GTTGAATGAGAGGAATCCAA+TGG 0.618258 tig0012395:-1720 None:intergenic
CTGGTGACATTATTGGTCAG+TGG 0.619927 tig0012395:+1774 Msa1351900:CDS
AGAACCATGCAGCTTAGCAA+AGG 0.620135 tig0012395:-1753 None:intergenic
TCTCAGACCAGTTGAATGAG+AGG 0.621991 tig0012395:-1730 None:intergenic
ACAGGCACAGGCACATCCGG+CGG 0.622496 tig0012395:+848 Msa1351900:CDS
GGAGGACTTGTGCTTGAAGG+TGG 0.626099 tig0012395:-421 None:intergenic
ATCTTCCCATCAGCTTCAGG+AGG 0.632762 tig0012395:+809 Msa1351900:CDS
GGTGATAATGGAACAGCTGG+TGG 0.636040 tig0012395:-268 None:intergenic
GGTGGTGGATAAGAATTCAC+TGG 0.638656 tig0012395:-352 None:intergenic
ACTGGGATTAGTGCTAGTAA+TGG 0.642339 tig0012395:-677 None:intergenic
GGTACTACATCATCTCCAGT+AGG 0.642872 tig0012395:+1678 Msa1351900:CDS
TGAATCTGATTATAGCCAAG+AGG 0.657778 tig0012395:-1991 None:intergenic
ATGTAAAAGTAAAATCGTAG+TGG 0.671959 tig0012395:-2325 None:intergenic
GATTGTTCACAAATAAACCA+AGG 0.679441 tig0012395:+544 Msa1351900:CDS
CCTCCTGAAGAAGAAGATGG+AGG 0.682773 tig0012395:-439 None:intergenic
CCACCATCACCATTATCAGG+AGG 0.700034 tig0012395:+370 Msa1351900:CDS
TTCAACAGCAGAAATAACTG+AGG 0.709826 tig0012395:+1967 Msa1351900:three_prime_UTR
TGTTCACAAATAAACCAAGG+TGG 0.741309 tig0012395:+547 Msa1351900:CDS
GCTGTTTGAAGTGTAGATTG+TGG 0.741949 tig0012395:-490 None:intergenic
TTGTATAACAAACTACAGCG+AGG 0.756170 tig0012395:-1839 None:intergenic
ACACCACCAACAGTTCCAGG+AGG 0.766003 tig0012395:+322 Msa1351900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTTATTTTATCTTT+TGG - tig0012395:1199-1218 None:intergenic 10.0%
!!! TTTGATTTTATTTTATCTTT+TGG - tig0012395:1199-1218 None:intergenic 10.0%
!!! TTTGTGTTTTAGAAATTTTA+AGG + tig0012395:1000-1019 Msa1351900:intron 15.0%
!!! TTTTCAACTTATTTTCATAA+GGG - tig0012395:1456-1475 None:intergenic 15.0%
!!! TTTGTGTTTTAGAAATTTTA+AGG + tig0012395:1000-1019 Msa1351900:intron 15.0%
!!! TTTTCAACTTATTTTCATAA+GGG - tig0012395:1456-1475 None:intergenic 15.0%
!! AAACTATTAAAGATCTTACT+GGG - tig0012395:697-716 None:intergenic 20.0%
!! AAAACTATTAAAGATCTTAC+TGG - tig0012395:698-717 None:intergenic 20.0%
!! CAAGCATAATATGAAAAATT+AGG - tig0012395:725-744 None:intergenic 20.0%
!! TTACTCAATTCAAATATCTA+CGG + tig0012395:945-964 Msa1351900:intron 20.0%
!!! TTACAAAAAAGACATTGATT+AGG - tig0012395:1026-1045 None:intergenic 20.0%
!! TAAGCTATAAGCTTAAAAAT+GGG - tig0012395:1279-1298 None:intergenic 20.0%
!! ATAAGCTATAAGCTTAAAAA+TGG - tig0012395:1280-1299 None:intergenic 20.0%
!!! ATATCTAAAACTAGCTTATA+GGG - tig0012395:1392-1411 None:intergenic 20.0%
!!! AATATCTAAAACTAGCTTAT+AGG - tig0012395:1393-1412 None:intergenic 20.0%
!!! GTTTTCAACTTATTTTCATA+AGG - tig0012395:1457-1476 None:intergenic 20.0%
!!! AATAAGTTGAAAACAACTTA+TGG + tig0012395:1463-1482 Msa1351900:intron 20.0%
!! AAACTATTAAAGATCTTACT+GGG - tig0012395:697-716 None:intergenic 20.0%
!! AAAACTATTAAAGATCTTAC+TGG - tig0012395:698-717 None:intergenic 20.0%
!! CAAGCATAATATGAAAAATT+AGG - tig0012395:725-744 None:intergenic 20.0%
!! TTACTCAATTCAAATATCTA+CGG + tig0012395:945-964 Msa1351900:intron 20.0%
!!! TTACAAAAAAGACATTGATT+AGG - tig0012395:1026-1045 None:intergenic 20.0%
!! TAAGCTATAAGCTTAAAAAT+GGG - tig0012395:1279-1298 None:intergenic 20.0%
!! ATAAGCTATAAGCTTAAAAA+TGG - tig0012395:1280-1299 None:intergenic 20.0%
!!! ATATCTAAAACTAGCTTATA+GGG - tig0012395:1392-1411 None:intergenic 20.0%
!!! AATATCTAAAACTAGCTTAT+AGG - tig0012395:1393-1412 None:intergenic 20.0%
!!! GTTTTCAACTTATTTTCATA+AGG - tig0012395:1457-1476 None:intergenic 20.0%
!!! AATAAGTTGAAAACAACTTA+TGG + tig0012395:1463-1482 Msa1351900:intron 20.0%
! AGAAATAGTTGAAAATGCAT+TGG - tig0012395:53-72 None:intergenic 25.0%
!! AATGCATTTTCAACTATTTC+TGG + tig0012395:52-71 Msa1351900:CDS 25.0%
!!! AGTATCTTATTGGCTTAAAA+AGG - tig0012395:926-945 None:intergenic 25.0%
! GAATTGAGTAAGTATCTTAT+TGG - tig0012395:936-955 None:intergenic 25.0%
!!! AAGTTGTTTTCACTTTTCAT+AGG + tig0012395:1238-1257 Msa1351900:intron 25.0%
!!! AGTTGTTTTCACTTTTCATA+GGG + tig0012395:1239-1258 Msa1351900:intron 25.0%
!! TATCTAAAACTAGCTTATAG+GGG - tig0012395:1391-1410 None:intergenic 25.0%
! GCTTAATACATCAATAGATA+TGG + tig0012395:1605-1624 Msa1351900:intron 25.0%
! ATTCAAGAAAATAAGTCATG+TGG + tig0012395:2094-2113 Msa1351900:three_prime_UTR 25.0%
! TTCAAGAAAATAAGTCATGT+GGG + tig0012395:2095-2114 Msa1351900:three_prime_UTR 25.0%
! CATATGTAAATCAACAACTA+TGG + tig0012395:2220-2239 Msa1351900:three_prime_UTR 25.0%
! ATGTAAAAGTAAAATCGTAG+TGG - tig0012395:2328-2347 None:intergenic 25.0%
! AGAAATAGTTGAAAATGCAT+TGG - tig0012395:53-72 None:intergenic 25.0%
!! AATGCATTTTCAACTATTTC+TGG + tig0012395:52-71 Msa1351900:CDS 25.0%
!!! AGTATCTTATTGGCTTAAAA+AGG - tig0012395:926-945 None:intergenic 25.0%
! GAATTGAGTAAGTATCTTAT+TGG - tig0012395:936-955 None:intergenic 25.0%
!!! AAGTTGTTTTCACTTTTCAT+AGG + tig0012395:1238-1257 Msa1351900:intron 25.0%
!!! AGTTGTTTTCACTTTTCATA+GGG + tig0012395:1239-1258 Msa1351900:intron 25.0%
!! TATCTAAAACTAGCTTATAG+GGG - tig0012395:1391-1410 None:intergenic 25.0%
! GCTTAATACATCAATAGATA+TGG + tig0012395:1605-1624 Msa1351900:intron 25.0%
! ATTCAAGAAAATAAGTCATG+TGG + tig0012395:2094-2113 Msa1351900:three_prime_UTR 25.0%
! TTCAAGAAAATAAGTCATGT+GGG + tig0012395:2095-2114 Msa1351900:three_prime_UTR 25.0%
! CATATGTAAATCAACAACTA+TGG + tig0012395:2220-2239 Msa1351900:three_prime_UTR 25.0%
! ATGTAAAAGTAAAATCGTAG+TGG - tig0012395:2328-2347 None:intergenic 25.0%
!! GCTACTTTCAAATTCTTTTC+AGG + tig0012395:25-44 Msa1351900:CDS 30.0%
TTGTAACAGATCACAAGATT+TGG + tig0012395:125-144 Msa1351900:CDS 30.0%
GATTGTTCACAAATAAACCA+AGG + tig0012395:544-563 Msa1351900:CDS 30.0%
!! GGAATGAGAACTTTGAAATA+TGG + tig0012395:1063-1082 Msa1351900:intron 30.0%
AGCATAATTTAGACCTTGTT+TGG + tig0012395:1100-1119 Msa1351900:intron 30.0%
TTAAGCTGTATATCCAAACA+AGG - tig0012395:1116-1135 None:intergenic 30.0%
TTGACTAAAAAAGACCAGTT+TGG + tig0012395:1315-1334 Msa1351900:intron 30.0%
!!! TTCTGAAACTGTTTTAGACA+TGG - tig0012395:1489-1508 None:intergenic 30.0%
ACATCAATAGATATGGAACA+AGG + tig0012395:1612-1631 Msa1351900:intron 30.0%
CATCAATAGATATGGAACAA+GGG + tig0012395:1613-1632 Msa1351900:intron 30.0%
TGTTATACAATTCTACAAGC+AGG + tig0012395:1852-1871 Msa1351900:three_prime_UTR 30.0%
TAAATCAACAACTATGGTGT+TGG + tig0012395:2226-2245 Msa1351900:three_prime_UTR 30.0%
!! ACTATGGTGTTGGTAAAATT+TGG + tig0012395:2236-2255 Msa1351900:three_prime_UTR 30.0%
TCTGAATTTGCGAAAATGAA+AGG - tig0012395:2355-2374 None:intergenic 30.0%
AATGGAAAATGCATGCATTT+AGG - tig0012395:2388-2407 None:intergenic 30.0%
!! GCTACTTTCAAATTCTTTTC+AGG + tig0012395:25-44 Msa1351900:CDS 30.0%
TTGTAACAGATCACAAGATT+TGG + tig0012395:125-144 Msa1351900:CDS 30.0%
GATTGTTCACAAATAAACCA+AGG + tig0012395:544-563 Msa1351900:CDS 30.0%
!! GGAATGAGAACTTTGAAATA+TGG + tig0012395:1063-1082 Msa1351900:intron 30.0%
AGCATAATTTAGACCTTGTT+TGG + tig0012395:1100-1119 Msa1351900:intron 30.0%
TTAAGCTGTATATCCAAACA+AGG - tig0012395:1116-1135 None:intergenic 30.0%
TTGACTAAAAAAGACCAGTT+TGG + tig0012395:1315-1334 Msa1351900:intron 30.0%
!!! TTCTGAAACTGTTTTAGACA+TGG - tig0012395:1489-1508 None:intergenic 30.0%
ACATCAATAGATATGGAACA+AGG + tig0012395:1612-1631 Msa1351900:intron 30.0%
CATCAATAGATATGGAACAA+GGG + tig0012395:1613-1632 Msa1351900:intron 30.0%
TGTTATACAATTCTACAAGC+AGG + tig0012395:1852-1871 Msa1351900:three_prime_UTR 30.0%
TAAATCAACAACTATGGTGT+TGG + tig0012395:2226-2245 Msa1351900:three_prime_UTR 30.0%
!! ACTATGGTGTTGGTAAAATT+TGG + tig0012395:2236-2255 Msa1351900:three_prime_UTR 30.0%
TCTGAATTTGCGAAAATGAA+AGG - tig0012395:2355-2374 None:intergenic 30.0%
AATGGAAAATGCATGCATTT+AGG - tig0012395:2388-2407 None:intergenic 30.0%
!!! TTGAGATCTTGCTTTTTGGA+AGG - tig0012395:87-106 None:intergenic 35.0%
!!! GACTTTGAGATCTTGCTTTT+TGG - tig0012395:91-110 None:intergenic 35.0%
ATCTACACTTCAAACAGCAT+TGG + tig0012395:495-514 Msa1351900:CDS 35.0%
TGTTCACAAATAAACCAAGG+TGG + tig0012395:547-566 Msa1351900:CDS 35.0%
!!! GCATGGTTTTGAAGTGAATT+TGG - tig0012395:586-605 None:intergenic 35.0%
TGTTGAATGCAAAAGAAGCA+TGG - tig0012395:603-622 None:intergenic 35.0%
! CTCTCTTTTGAATTGTGACT+AGG + tig0012395:773-792 Msa1351900:intron 35.0%
TTTGAATTGTGACTAGGTTC+TGG + tig0012395:779-798 Msa1351900:intron 35.0%
TAATGAAAGCTTTCCCTTCA+CGG - tig0012395:885-904 None:intergenic 35.0%
CAAAGTTCTCATTCCATCTA+AGG - tig0012395:1058-1077 None:intergenic 35.0%
TATAGGGGATGAACATATAG+TGG - tig0012395:1376-1395 None:intergenic 35.0%
TTGTATAACAAACTACAGCG+AGG - tig0012395:1842-1861 None:intergenic 35.0%
TAGAAAGACAGTAGAGTAGT+AGG + tig0012395:1877-1896 Msa1351900:three_prime_UTR 35.0%
AGAAAGACAGTAGAGTAGTA+GGG + tig0012395:1878-1897 Msa1351900:three_prime_UTR 35.0%
AAGCTTGAGTAAACAGATCA+AGG - tig0012395:1935-1954 None:intergenic 35.0%
TTCAACAGCAGAAATAACTG+AGG + tig0012395:1967-1986 Msa1351900:three_prime_UTR 35.0%
TGAATCTGATTATAGCCAAG+AGG - tig0012395:1994-2013 None:intergenic 35.0%
TCTTGGCTATAATCAGATTC+AGG + tig0012395:1993-2012 Msa1351900:three_prime_UTR 35.0%
CTATAATCAGATTCAGGAAC+TGG + tig0012395:1999-2018 Msa1351900:three_prime_UTR 35.0%
! AGTGTGTGCATTGTTTGTTT+TGG + tig0012395:2047-2066 Msa1351900:three_prime_UTR 35.0%
AAAATAAGTCATGTGGGTAC+TGG + tig0012395:2101-2120 Msa1351900:three_prime_UTR 35.0%
TCATTGCACTAACTAGTAGA+TGG + tig0012395:2135-2154 Msa1351900:three_prime_UTR 35.0%
GCTTACAAATCCAACATCTT+AGG + tig0012395:2293-2312 Msa1351900:three_prime_UTR 35.0%
!!! TTGAGATCTTGCTTTTTGGA+AGG - tig0012395:87-106 None:intergenic 35.0%
!!! GACTTTGAGATCTTGCTTTT+TGG - tig0012395:91-110 None:intergenic 35.0%
ATCTACACTTCAAACAGCAT+TGG + tig0012395:495-514 Msa1351900:CDS 35.0%
TGTTCACAAATAAACCAAGG+TGG + tig0012395:547-566 Msa1351900:CDS 35.0%
!!! GCATGGTTTTGAAGTGAATT+TGG - tig0012395:586-605 None:intergenic 35.0%
TGTTGAATGCAAAAGAAGCA+TGG - tig0012395:603-622 None:intergenic 35.0%
! CTCTCTTTTGAATTGTGACT+AGG + tig0012395:773-792 Msa1351900:intron 35.0%
TTTGAATTGTGACTAGGTTC+TGG + tig0012395:779-798 Msa1351900:intron 35.0%
TAATGAAAGCTTTCCCTTCA+CGG - tig0012395:885-904 None:intergenic 35.0%
CAAAGTTCTCATTCCATCTA+AGG - tig0012395:1058-1077 None:intergenic 35.0%
TATAGGGGATGAACATATAG+TGG - tig0012395:1376-1395 None:intergenic 35.0%
TTGTATAACAAACTACAGCG+AGG - tig0012395:1842-1861 None:intergenic 35.0%
TAGAAAGACAGTAGAGTAGT+AGG + tig0012395:1877-1896 Msa1351900:three_prime_UTR 35.0%
AGAAAGACAGTAGAGTAGTA+GGG + tig0012395:1878-1897 Msa1351900:three_prime_UTR 35.0%
AAGCTTGAGTAAACAGATCA+AGG - tig0012395:1935-1954 None:intergenic 35.0%
TTCAACAGCAGAAATAACTG+AGG + tig0012395:1967-1986 Msa1351900:three_prime_UTR 35.0%
TGAATCTGATTATAGCCAAG+AGG - tig0012395:1994-2013 None:intergenic 35.0%
TCTTGGCTATAATCAGATTC+AGG + tig0012395:1993-2012 Msa1351900:three_prime_UTR 35.0%
CTATAATCAGATTCAGGAAC+TGG + tig0012395:1999-2018 Msa1351900:three_prime_UTR 35.0%
! AGTGTGTGCATTGTTTGTTT+TGG + tig0012395:2047-2066 Msa1351900:three_prime_UTR 35.0%
AAAATAAGTCATGTGGGTAC+TGG + tig0012395:2101-2120 Msa1351900:three_prime_UTR 35.0%
TCATTGCACTAACTAGTAGA+TGG + tig0012395:2135-2154 Msa1351900:three_prime_UTR 35.0%
GCTTACAAATCCAACATCTT+AGG + tig0012395:2293-2312 Msa1351900:three_prime_UTR 35.0%
!! CAGTGATGATTGTTGGTGAT+GGG - tig0012395:171-190 None:intergenic 40.0%
! ACAGTGATGATTGTTGGTGA+TGG - tig0012395:172-191 None:intergenic 40.0%
GAAGGAACAGTGATGATTGT+TGG - tig0012395:178-197 None:intergenic 40.0%
AGAAACTGTTGTGACAGGAT+TGG - tig0012395:203-222 None:intergenic 40.0%
ACAGTTTCTCCAACAAATCC+AGG + tig0012395:214-233 Msa1351900:CDS 40.0%
!! GCTGGATTTGTTGGTGATAA+TGG - tig0012395:283-302 None:intergenic 40.0%
!! GGTGAATTTGCTGGATTTGT+TGG - tig0012395:292-311 None:intergenic 40.0%
! GCTGTTTGAAGTGTAGATTG+TGG - tig0012395:493-512 None:intergenic 40.0%
GACTATGCTTGTGGAACAAA+AGG + tig0012395:517-536 Msa1351900:CDS 40.0%
! ACTGGGATTAGTGCTAGTAA+TGG - tig0012395:680-699 None:intergenic 40.0%
GTAACCAAACCTACCTTAGA+TGG + tig0012395:1042-1061 Msa1351900:intron 40.0%
! CATTCCATCTAAGGTAGGTT+TGG - tig0012395:1049-1068 None:intergenic 40.0%
! GTTCTCATTCCATCTAAGGT+AGG - tig0012395:1054-1073 None:intergenic 40.0%
! CACTTTTCATAGGGTCTGTA+TGG + tig0012395:1248-1267 Msa1351900:intron 40.0%
CACATTAAGCGAGTCTGTTT+GGG - tig0012395:1548-1567 None:intergenic 40.0%
ACACATTAAGCGAGTCTGTT+TGG - tig0012395:1549-1568 None:intergenic 40.0%
!! GAACAAGGGAGTACTAATCA+TGG + tig0012395:1627-1646 Msa1351900:intron 40.0%
! ACATCATCTCCAGTAGGTTT+TGG + tig0012395:1684-1703 Msa1351900:CDS 40.0%
! CATCATCTCCAGTAGGTTTT+GGG + tig0012395:1685-1704 Msa1351900:CDS 40.0%
GTTGAATGAGAGGAATCCAA+TGG - tig0012395:1723-1742 None:intergenic 40.0%
TTGGATTCCTCTCATTCAAC+TGG + tig0012395:1723-1742 Msa1351900:CDS 40.0%
!! CATGGTTCTGGTGACATTAT+TGG + tig0012395:1767-1786 Msa1351900:CDS 40.0%
TTATTGGTCAGTGGAAGACT+CGG + tig0012395:1783-1802 Msa1351900:CDS 40.0%
AGAGCATGGAAGCTAACTAT+GGG - tig0012395:1815-1834 None:intergenic 40.0%
TAGAGCATGGAAGCTAACTA+TGG - tig0012395:1816-1835 None:intergenic 40.0%
AGAAATAACTGAGGACCTCT+TGG + tig0012395:1976-1995 Msa1351900:three_prime_UTR 40.0%
TTCAGGAACTGGAGTAGTTT+AGG + tig0012395:2010-2029 Msa1351900:three_prime_UTR 40.0%
GTAAAATCGTAGTGGTCTGA+TGG - tig0012395:2320-2339 None:intergenic 40.0%
!! CAGTGATGATTGTTGGTGAT+GGG - tig0012395:171-190 None:intergenic 40.0%
! ACAGTGATGATTGTTGGTGA+TGG - tig0012395:172-191 None:intergenic 40.0%
GAAGGAACAGTGATGATTGT+TGG - tig0012395:178-197 None:intergenic 40.0%
AGAAACTGTTGTGACAGGAT+TGG - tig0012395:203-222 None:intergenic 40.0%
ACAGTTTCTCCAACAAATCC+AGG + tig0012395:214-233 Msa1351900:CDS 40.0%
!! GCTGGATTTGTTGGTGATAA+TGG - tig0012395:283-302 None:intergenic 40.0%
!! GGTGAATTTGCTGGATTTGT+TGG - tig0012395:292-311 None:intergenic 40.0%
! GCTGTTTGAAGTGTAGATTG+TGG - tig0012395:493-512 None:intergenic 40.0%
GACTATGCTTGTGGAACAAA+AGG + tig0012395:517-536 Msa1351900:CDS 40.0%
! ACTGGGATTAGTGCTAGTAA+TGG - tig0012395:680-699 None:intergenic 40.0%
GTAACCAAACCTACCTTAGA+TGG + tig0012395:1042-1061 Msa1351900:intron 40.0%
! CATTCCATCTAAGGTAGGTT+TGG - tig0012395:1049-1068 None:intergenic 40.0%
! GTTCTCATTCCATCTAAGGT+AGG - tig0012395:1054-1073 None:intergenic 40.0%
! CACTTTTCATAGGGTCTGTA+TGG + tig0012395:1248-1267 Msa1351900:intron 40.0%
CACATTAAGCGAGTCTGTTT+GGG - tig0012395:1548-1567 None:intergenic 40.0%
ACACATTAAGCGAGTCTGTT+TGG - tig0012395:1549-1568 None:intergenic 40.0%
!! GAACAAGGGAGTACTAATCA+TGG + tig0012395:1627-1646 Msa1351900:intron 40.0%
! ACATCATCTCCAGTAGGTTT+TGG + tig0012395:1684-1703 Msa1351900:CDS 40.0%
! CATCATCTCCAGTAGGTTTT+GGG + tig0012395:1685-1704 Msa1351900:CDS 40.0%
GTTGAATGAGAGGAATCCAA+TGG - tig0012395:1723-1742 None:intergenic 40.0%
TTGGATTCCTCTCATTCAAC+TGG + tig0012395:1723-1742 Msa1351900:CDS 40.0%
!! CATGGTTCTGGTGACATTAT+TGG + tig0012395:1767-1786 Msa1351900:CDS 40.0%
TTATTGGTCAGTGGAAGACT+CGG + tig0012395:1783-1802 Msa1351900:CDS 40.0%
AGAGCATGGAAGCTAACTAT+GGG - tig0012395:1815-1834 None:intergenic 40.0%
TAGAGCATGGAAGCTAACTA+TGG - tig0012395:1816-1835 None:intergenic 40.0%
AGAAATAACTGAGGACCTCT+TGG + tig0012395:1976-1995 Msa1351900:three_prime_UTR 40.0%
TTCAGGAACTGGAGTAGTTT+AGG + tig0012395:2010-2029 Msa1351900:three_prime_UTR 40.0%
GTAAAATCGTAGTGGTCTGA+TGG - tig0012395:2320-2339 None:intergenic 40.0%
!! GTTGTGACAGGATTGGTTGA+AGG - tig0012395:196-215 None:intergenic 45.0%
GTTGGAGAAACTGTTGTGAC+AGG - tig0012395:208-227 None:intergenic 45.0%
GTAGTAGAAGGAACTGTGAC+AGG - tig0012395:247-266 None:intergenic 45.0%
!! GTTGGTGATAATGGAACAGC+TGG - tig0012395:274-293 None:intergenic 45.0%
!! ACAGGTACTGGTGAATTTGC+TGG - tig0012395:301-320 None:intergenic 45.0%
!! GGTGGTGGATAAGAATTCAC+TGG - tig0012395:355-374 None:intergenic 45.0%
!! GTTCCTCCTGATAATGGTGA+TGG - tig0012395:376-395 None:intergenic 45.0%
TCACCATTATCAGGAGGAAC+AGG + tig0012395:376-395 Msa1351900:CDS 45.0%
GTTCCTGTTCCTCCTGATAA+TGG - tig0012395:382-401 None:intergenic 45.0%
AGTCCTCCATCTTCTTCTTC+AGG + tig0012395:436-455 Msa1351900:CDS 45.0%
CTACCTCCTGAAGAAGAAGA+TGG - tig0012395:445-464 None:intergenic 45.0%
AGTGTAGATTGTGGTGTTCC+AGG - tig0012395:484-503 None:intergenic 45.0%
! ACAGCATTGGACTATGCTTG+TGG + tig0012395:508-527 Msa1351900:CDS 45.0%
GATTGTAACAGCTACCACCT+TGG - tig0012395:564-583 None:intergenic 45.0%
CAACAAGCTGTGACTTTGGA+GGG + tig0012395:647-666 Msa1351900:CDS 45.0%
! AACAAGCTGTGACTTTGGAG+GGG + tig0012395:648-667 Msa1351900:CDS 45.0%
GAAGGTGACGTTTACCAAAC+TGG - tig0012395:1332-1351 None:intergenic 45.0%
! AGATAGCTTGTGGCTCTGTT+TGG - tig0012395:1428-1447 None:intergenic 45.0%
AACAGAGCCACAAGCTATCT+TGG + tig0012395:1428-1447 Msa1351900:intron 45.0%
! TAAGCGAGTCTGTTTGGGAT+TGG - tig0012395:1543-1562 None:intergenic 45.0%
! ATTCCCATCTTTTGCAGCTC+TGG + tig0012395:1657-1676 Msa1351900:intron 45.0%
GTACCAGAGCTGCAAAAGAT+GGG - tig0012395:1663-1682 None:intergenic 45.0%
AGTACCAGAGCTGCAAAAGA+TGG - tig0012395:1664-1683 None:intergenic 45.0%
GGTACTACATCATCTCCAGT+AGG + tig0012395:1678-1697 Msa1351900:CDS 45.0%
GAATCCAATGGAGAGCTTTG+CGG - tig0012395:1711-1730 None:intergenic 45.0%
TCTCAGACCAGTTGAATGAG+AGG - tig0012395:1733-1752 None:intergenic 45.0%
AGAACCATGCAGCTTAGCAA+AGG - tig0012395:1756-1775 None:intergenic 45.0%
TTTGCTAAGCTGCATGGTTC+TGG + tig0012395:1755-1774 Msa1351900:CDS 45.0%
CTGGTGACATTATTGGTCAG+TGG + tig0012395:1774-1793 Msa1351900:CDS 45.0%
AGAGTAGTAGGGAGAGTTTG+TGG + tig0012395:1889-1908 Msa1351900:three_prime_UTR 45.0%
! GTCTGATGGACCTAAGATGT+TGG - tig0012395:2306-2325 None:intergenic 45.0%
!! GTTGTGACAGGATTGGTTGA+AGG - tig0012395:196-215 None:intergenic 45.0%
GTTGGAGAAACTGTTGTGAC+AGG - tig0012395:208-227 None:intergenic 45.0%
GTAGTAGAAGGAACTGTGAC+AGG - tig0012395:247-266 None:intergenic 45.0%
!! GTTGGTGATAATGGAACAGC+TGG - tig0012395:274-293 None:intergenic 45.0%
!! ACAGGTACTGGTGAATTTGC+TGG - tig0012395:301-320 None:intergenic 45.0%
!! GGTGGTGGATAAGAATTCAC+TGG - tig0012395:355-374 None:intergenic 45.0%
!! GTTCCTCCTGATAATGGTGA+TGG - tig0012395:376-395 None:intergenic 45.0%
TCACCATTATCAGGAGGAAC+AGG + tig0012395:376-395 Msa1351900:CDS 45.0%
GTTCCTGTTCCTCCTGATAA+TGG - tig0012395:382-401 None:intergenic 45.0%
AGTCCTCCATCTTCTTCTTC+AGG + tig0012395:436-455 Msa1351900:CDS 45.0%
CTACCTCCTGAAGAAGAAGA+TGG - tig0012395:445-464 None:intergenic 45.0%
AGTGTAGATTGTGGTGTTCC+AGG - tig0012395:484-503 None:intergenic 45.0%
! ACAGCATTGGACTATGCTTG+TGG + tig0012395:508-527 Msa1351900:CDS 45.0%
GATTGTAACAGCTACCACCT+TGG - tig0012395:564-583 None:intergenic 45.0%
CAACAAGCTGTGACTTTGGA+GGG + tig0012395:647-666 Msa1351900:CDS 45.0%
! AACAAGCTGTGACTTTGGAG+GGG + tig0012395:648-667 Msa1351900:CDS 45.0%
GAAGGTGACGTTTACCAAAC+TGG - tig0012395:1332-1351 None:intergenic 45.0%
! AGATAGCTTGTGGCTCTGTT+TGG - tig0012395:1428-1447 None:intergenic 45.0%
AACAGAGCCACAAGCTATCT+TGG + tig0012395:1428-1447 Msa1351900:intron 45.0%
! TAAGCGAGTCTGTTTGGGAT+TGG - tig0012395:1543-1562 None:intergenic 45.0%
! ATTCCCATCTTTTGCAGCTC+TGG + tig0012395:1657-1676 Msa1351900:intron 45.0%
GTACCAGAGCTGCAAAAGAT+GGG - tig0012395:1663-1682 None:intergenic 45.0%
AGTACCAGAGCTGCAAAAGA+TGG - tig0012395:1664-1683 None:intergenic 45.0%
GGTACTACATCATCTCCAGT+AGG + tig0012395:1678-1697 Msa1351900:CDS 45.0%
GAATCCAATGGAGAGCTTTG+CGG - tig0012395:1711-1730 None:intergenic 45.0%
TCTCAGACCAGTTGAATGAG+AGG - tig0012395:1733-1752 None:intergenic 45.0%
AGAACCATGCAGCTTAGCAA+AGG - tig0012395:1756-1775 None:intergenic 45.0%
TTTGCTAAGCTGCATGGTTC+TGG + tig0012395:1755-1774 Msa1351900:CDS 45.0%
CTGGTGACATTATTGGTCAG+TGG + tig0012395:1774-1793 Msa1351900:CDS 45.0%
AGAGTAGTAGGGAGAGTTTG+TGG + tig0012395:1889-1908 Msa1351900:three_prime_UTR 45.0%
! GTCTGATGGACCTAAGATGT+TGG - tig0012395:2306-2325 None:intergenic 45.0%
! GGTGTTGCACCTGGATTTGT+TGG - tig0012395:226-245 None:intergenic 50.0%
ACAGCTGGTGGAGTAGTAGA+AGG - tig0012395:259-278 None:intergenic 50.0%
!! GGTGATAATGGAACAGCTGG+TGG - tig0012395:271-290 None:intergenic 50.0%
!! GTTGGTGGTGTAACAGGTAC+TGG - tig0012395:313-332 None:intergenic 50.0%
!! GGAACTGTTGGTGGTGTAAC+AGG - tig0012395:319-338 None:intergenic 50.0%
GTTACACCACCAACAGTTCC+AGG + tig0012395:319-338 Msa1351900:CDS 50.0%
GTTGTTCCTCCTGGAACTGT+TGG - tig0012395:331-350 None:intergenic 50.0%
!! TTCACTGGTGTTGTTCCTCC+TGG - tig0012395:340-359 None:intergenic 50.0%
!! CCTGATAATGGTGATGGTGG+TGG - tig0012395:370-389 None:intergenic 50.0%
CCACCACCATCACCATTATC+AGG + tig0012395:367-386 Msa1351900:CDS 50.0%
!! CCTCCTGATAATGGTGATGG+TGG - tig0012395:373-392 None:intergenic 50.0%
CCACCATCACCATTATCAGG+AGG + tig0012395:370-389 Msa1351900:CDS 50.0%
GAAGGTGGAGGATTTGTGAC+AGG - tig0012395:409-428 None:intergenic 50.0%
GATGGAGGACTTGTGCTTGA+AGG - tig0012395:427-446 None:intergenic 50.0%
CCTCCTGAAGAAGAAGATGG+AGG - tig0012395:442-461 None:intergenic 50.0%
CCTCCATCTTCTTCTTCAGG+AGG + tig0012395:439-458 Msa1351900:CDS 50.0%
CTTCTTCTTCAGGAGGTAGC+TGG + tig0012395:446-465 Msa1351900:CDS 50.0%
!! AAGTCACAGCTTGTTGCAGC+AGG - tig0012395:643-662 None:intergenic 50.0%
GCTGCAACAAGCTGTGACTT+TGG + tig0012395:643-662 Msa1351900:CDS 50.0%
GCAACAAGCTGTGACTTTGG+AGG + tig0012395:646-665 Msa1351900:CDS 50.0%
TGAAGCTGATGGGAAGATGC+AGG - tig0012395:807-826 None:intergenic 50.0%
TGCATCTTCCCATCAGCTTC+AGG + tig0012395:806-825 Msa1351900:CDS 50.0%
ATCTTCCCATCAGCTTCAGG+AGG + tig0012395:809-828 Msa1351900:CDS 50.0%
GGGTAAGTGAGAACCGTGAA+GGG + tig0012395:869-888 Msa1351900:intron 50.0%
GCTGATAAGCTCGTGTGAGA+AGG - tig0012395:1350-1369 None:intergenic 50.0%
AAGGGCTCCAAGATAGCTTG+TGG - tig0012395:1438-1457 None:intergenic 50.0%
GAGACCTTTGCTAAGCTGCA+TGG + tig0012395:1749-1768 Msa1351900:CDS 50.0%
TACAGCGAGGAGATAGAGCA+TGG - tig0012395:1829-1848 None:intergenic 50.0%
! GGTGTTGCACCTGGATTTGT+TGG - tig0012395:226-245 None:intergenic 50.0%
ACAGCTGGTGGAGTAGTAGA+AGG - tig0012395:259-278 None:intergenic 50.0%
!! GGTGATAATGGAACAGCTGG+TGG - tig0012395:271-290 None:intergenic 50.0%
!! GTTGGTGGTGTAACAGGTAC+TGG - tig0012395:313-332 None:intergenic 50.0%
!! GGAACTGTTGGTGGTGTAAC+AGG - tig0012395:319-338 None:intergenic 50.0%
GTTACACCACCAACAGTTCC+AGG + tig0012395:319-338 Msa1351900:CDS 50.0%
GTTGTTCCTCCTGGAACTGT+TGG - tig0012395:331-350 None:intergenic 50.0%
!! TTCACTGGTGTTGTTCCTCC+TGG - tig0012395:340-359 None:intergenic 50.0%
!! CCTGATAATGGTGATGGTGG+TGG - tig0012395:370-389 None:intergenic 50.0%
CCACCACCATCACCATTATC+AGG + tig0012395:367-386 Msa1351900:CDS 50.0%
!! CCTCCTGATAATGGTGATGG+TGG - tig0012395:373-392 None:intergenic 50.0%
CCACCATCACCATTATCAGG+AGG + tig0012395:370-389 Msa1351900:CDS 50.0%
GAAGGTGGAGGATTTGTGAC+AGG - tig0012395:409-428 None:intergenic 50.0%
GATGGAGGACTTGTGCTTGA+AGG - tig0012395:427-446 None:intergenic 50.0%
CCTCCTGAAGAAGAAGATGG+AGG - tig0012395:442-461 None:intergenic 50.0%
CCTCCATCTTCTTCTTCAGG+AGG + tig0012395:439-458 Msa1351900:CDS 50.0%
CTTCTTCTTCAGGAGGTAGC+TGG + tig0012395:446-465 Msa1351900:CDS 50.0%
!! AAGTCACAGCTTGTTGCAGC+AGG - tig0012395:643-662 None:intergenic 50.0%
GCTGCAACAAGCTGTGACTT+TGG + tig0012395:643-662 Msa1351900:CDS 50.0%
GCAACAAGCTGTGACTTTGG+AGG + tig0012395:646-665 Msa1351900:CDS 50.0%
TGAAGCTGATGGGAAGATGC+AGG - tig0012395:807-826 None:intergenic 50.0%
TGCATCTTCCCATCAGCTTC+AGG + tig0012395:806-825 Msa1351900:CDS 50.0%
ATCTTCCCATCAGCTTCAGG+AGG + tig0012395:809-828 Msa1351900:CDS 50.0%
GGGTAAGTGAGAACCGTGAA+GGG + tig0012395:869-888 Msa1351900:intron 50.0%
GCTGATAAGCTCGTGTGAGA+AGG - tig0012395:1350-1369 None:intergenic 50.0%
AAGGGCTCCAAGATAGCTTG+TGG - tig0012395:1438-1457 None:intergenic 50.0%
GAGACCTTTGCTAAGCTGCA+TGG + tig0012395:1749-1768 Msa1351900:CDS 50.0%
TACAGCGAGGAGATAGAGCA+TGG - tig0012395:1829-1848 None:intergenic 50.0%
! ACTGTGACAGGTGTTGCACC+TGG - tig0012395:235-254 None:intergenic 55.0%
ACACCACCAACAGTTCCAGG+AGG + tig0012395:322-341 Msa1351900:CDS 55.0%
GTTCCTCCTGGAACTGTTGG+TGG - tig0012395:328-347 None:intergenic 55.0%
GGACTTGTGCTTGAAGGTGG+AGG - tig0012395:421-440 None:intergenic 55.0%
GGAGGACTTGTGCTTGAAGG+TGG - tig0012395:424-443 None:intergenic 55.0%
TTCAGGAGGTAGCTGGTGTG+TGG + tig0012395:453-472 Msa1351900:CDS 55.0%
AGCTGGTGTGTGGCTAAACC+TGG + tig0012395:463-482 Msa1351900:CDS 55.0%
TTCCCATCAGCTTCAGGAGG+AGG + tig0012395:812-831 Msa1351900:CDS 55.0%
CTCCTCCTCCTGAAGCTGAT+GGG - tig0012395:817-836 None:intergenic 55.0%
CGGGTAAGTGAGAACCGTGA+AGG + tig0012395:868-887 Msa1351900:intron 55.0%
CTTTGCGGCCCAAAACCTAC+TGG - tig0012395:1696-1715 None:intergenic 55.0%
! ACTGTGACAGGTGTTGCACC+TGG - tig0012395:235-254 None:intergenic 55.0%
ACACCACCAACAGTTCCAGG+AGG + tig0012395:322-341 Msa1351900:CDS 55.0%
GTTCCTCCTGGAACTGTTGG+TGG - tig0012395:328-347 None:intergenic 55.0%
GGACTTGTGCTTGAAGGTGG+AGG - tig0012395:421-440 None:intergenic 55.0%
GGAGGACTTGTGCTTGAAGG+TGG - tig0012395:424-443 None:intergenic 55.0%
TTCAGGAGGTAGCTGGTGTG+TGG + tig0012395:453-472 Msa1351900:CDS 55.0%
AGCTGGTGTGTGGCTAAACC+TGG + tig0012395:463-482 Msa1351900:CDS 55.0%
TTCCCATCAGCTTCAGGAGG+AGG + tig0012395:812-831 Msa1351900:CDS 55.0%
CTCCTCCTCCTGAAGCTGAT+GGG - tig0012395:817-836 None:intergenic 55.0%
CGGGTAAGTGAGAACCGTGA+AGG + tig0012395:868-887 Msa1351900:intron 55.0%
CTTTGCGGCCCAAAACCTAC+TGG - tig0012395:1696-1715 None:intergenic 55.0%
GCTCCTCCTCCTGAAGCTGA+TGG - tig0012395:818-837 None:intergenic 60.0%
TCAGCTTCAGGAGGAGGAGC+TGG + tig0012395:818-837 Msa1351900:CDS 60.0%
CACGGTTCTCACTTACCCGC+CGG - tig0012395:867-886 None:intergenic 60.0%
TGGGCCGCAAAGCTCTCCAT+TGG + tig0012395:1704-1723 Msa1351900:CDS 60.0%
GCTCCTCCTCCTGAAGCTGA+TGG - tig0012395:818-837 None:intergenic 60.0%
TCAGCTTCAGGAGGAGGAGC+TGG + tig0012395:818-837 Msa1351900:CDS 60.0%
CACGGTTCTCACTTACCCGC+CGG - tig0012395:867-886 None:intergenic 60.0%
TGGGCCGCAAAGCTCTCCAT+TGG + tig0012395:1704-1723 Msa1351900:CDS 60.0%
GGCACAGGCACAGGCACATC+CGG + tig0012395:845-864 Msa1351900:CDS 65.0%
ACAGGCACAGGCACATCCGG+CGG + tig0012395:848-867 Msa1351900:CDS 65.0%
GGCACAGGCACAGGCACATC+CGG + tig0012395:845-864 Msa1351900:CDS 65.0%
ACAGGCACAGGCACATCCGG+CGG + tig0012395:848-867 Msa1351900:CDS 65.0%
TCAGGAGGAGGAGCTGGCGC+AGG + tig0012395:824-843 Msa1351900:CDS 70.0%
CAGGCACAGGCACATCCGGC+GGG + tig0012395:849-868 Msa1351900:CDS 70.0%
TCAGGAGGAGGAGCTGGCGC+AGG + tig0012395:824-843 Msa1351900:CDS 70.0%
CAGGCACAGGCACATCCGGC+GGG + tig0012395:849-868 Msa1351900:CDS 70.0%
GGAGGAGCTGGCGCAGGCAC+AGG + tig0012395:830-849 Msa1351900:CDS 75.0%
GCTGGCGCAGGCACAGGCAC+AGG + tig0012395:836-855 Msa1351900:CDS 75.0%
GGAGGAGCTGGCGCAGGCAC+AGG + tig0012395:830-849 Msa1351900:CDS 75.0%
GCTGGCGCAGGCACAGGCAC+AGG + tig0012395:836-855 Msa1351900:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0012395 gene 25 2415 25 ID=Msa1351900;Name=Msa1351900
tig0012395 mRNA 25 2415 25 ID=Msa1351900-mRNA-1;Parent=Msa1351900;Name=Msa1351900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|1|1|1|1|3|597|297
tig0012395 exon 25 697 25 ID=Msa1351900-mRNA-1:exon:7910;Parent=Msa1351900-mRNA-1
tig0012395 exon 795 870 795 ID=Msa1351900-mRNA-1:exon:7911;Parent=Msa1351900-mRNA-1
tig0012395 exon 1674 2415 1674 ID=Msa1351900-mRNA-1:exon:7912;Parent=Msa1351900-mRNA-1
tig0012395 CDS 25 697 25 ID=Msa1351900-mRNA-1:cds;Parent=Msa1351900-mRNA-1
tig0012395 CDS 795 870 795 ID=Msa1351900-mRNA-1:cds;Parent=Msa1351900-mRNA-1
tig0012395 CDS 1674 1818 1674 ID=Msa1351900-mRNA-1:cds;Parent=Msa1351900-mRNA-1
tig0012395 three_prime_UTR 1819 2415 1819 ID=Msa1351900-mRNA-1:three_prime_utr;Parent=Msa1351900-mRNA-1
Gene Sequence

>Msa1351900

GCTACTTTCAAATTCTTTTCAGGAACCAATGCATTTTCAACTATTTCTGGAGCAAGATTCCTTCCAAAAAGCAAGATCTCAAAGTCTCAGATTCAGTTTCTTGTAACAGATCACAAGATTTGGAGAAAACTTCTAGACACAAACCCATCACCAACAATCATCACTGTTCCTTCAACCAATCCTGTCACAACAGTTTCTCCAACAAATCCAGGTGCAACACCTGTCACAGTTCCTTCTACTACTCCACCAGCTGTTCCATTATCACCAACAAATCCAGCAAATTCACCAGTACCTGTTACACCACCAACAGTTCCAGGAGGAACAACACCAGTGAATTCTTATCCACCACCATCACCATTATCAGGAGGAACAGGAACTGTTCCTGTCACAAATCCTCCACCTTCAAGCACAAGTCCTCCATCTTCTTCTTCAGGAGGTAGCTGGTGTGTGGCTAAACCTGGAACACCACAATCTACACTTCAAACAGCATTGGACTATGCTTGTGGAACAAAAGGAACTGATTGTTCACAAATAAACCAAGGTGGTAGCTGTTACAATCCAAATTCACTTCAAAACCATGCTTCTTTTGCATTCAACAGCTACTATGTAAAGAATCCTGCTGCAACAAGCTGTGACTTTGGAGGGGTTGCTACCATTACTAGCACTAATCCCAGTTCTGGTACCTGCATCTTCCCATCAGCTTCAGGAGGAGGAGCTGGCGCAGGCACAGGCACAGGCACATCCGGCGGCTCTGGTACTACATCATCTCCAGTAGGTTTTGGGCCGCAAAGCTCTCCATTGGATTCCTCTCATTCAACTGGTCTGAGACCTTTGCTAAGCTGCATGGTTCTGGTGACATTATTGGTCAGTGGAAGACTCGGCATGAGCCCATAG
Protein sequence

>Msa1351900

ATFKFFSGTNAFSTISGARFLPKSKISKSQIQFLVTDHKIWRKLLDTNPSPTIITVPSTNPVTTVSPTNPGATPVTVPSTTPPAVPLSPTNPANSPVPVTPPTVPGGTTPVNSYPPPSPLSGGTGTVPVTNPPPSSTSPPSSSSGGSWCVAKPGTPQSTLQTALDYACGTKGTDCSQINQGGSCYNPNSLQNHASFAFNSYYVKNPAATSCDFGGVATITSTNPSSGTCIFPSASGGGAGAGTGTGTSGGSGTTSSPVGFGPQSSPLDSSHSTGLRPLLSCMVLVTLLVSGRLGMSP*