AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0080820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0080820 MtrunA17_Chr1g0193131 94.787 633 33 0 1 633 1 633 0.0 1021
Msa0080820 MtrunA17_Chr7g0271411 63.402 194 50 8 318 503 64 244 7.75e-56 191
Msa0080820 MtrunA17_Chr3g0096451 47.799 159 60 4 346 503 60 196 8.33e-37 138
Msa0080820 MtrunA17_Chr5g0443331 54.545 110 49 1 402 510 90 199 7.02e-32 124
Msa0080820 MtrunA17_Chr2g0304061 41.589 214 82 6 331 511 46 249 3.20e-30 121
Msa0080820 MtrunA17_Chr2g0304061 48.819 127 48 2 420 546 158 267 5.95e-26 109
Msa0080820 MtrunA17_Chr8g0381081 53.636 110 47 3 405 512 44 151 7.29e-29 113
Msa0080820 MtrunA17_Chr8g0378371 60.227 88 33 1 418 505 31 116 8.00e-29 110
Msa0080820 MtrunA17_Chr1g0177171 57.609 92 37 2 416 505 402 493 4.20e-28 118
Msa0080820 MtrunA17_Chr8g0378361 45.038 131 60 2 419 548 29 148 2.72e-27 108
Msa0080820 MtrunA17_Chr5g0401301 26.984 441 205 16 87 502 66 414 9.79e-27 113
Msa0080820 MtrunA17_Chr4g0075031 50.485 103 49 2 412 512 374 476 1.28e-26 114
Msa0080820 MtrunA17_Chr8g0378381 49.438 89 44 1 416 503 28 116 8.04e-25 99.8
Msa0080820 MtrunA17_Chr8g0380231 65.217 69 23 1 429 496 26 94 8.91e-23 93.2
Msa0080820 MtrunA17_Chr8g0378401 49.398 83 41 1 416 497 28 110 1.67e-22 93.2
Msa0080820 MtrunA17_Chr1g0154991 48.889 90 45 1 419 507 19 108 2.13e-22 92.4
Msa0080820 MtrunA17_Chr3g0121271 46.000 100 52 2 418 515 360 459 2.83e-22 100
Msa0080820 MtrunA17_Chr3g0120401 50.575 87 42 1 420 505 157 243 5.07e-22 95.5
Msa0080820 MtrunA17_Chr5g0447651 51.613 93 43 2 412 502 22 114 5.75e-22 91.7
Msa0080820 MtrunA17_Chr8g0378621 48.421 95 47 2 420 512 364 458 1.87e-21 98.2
Msa0080820 MtrunA17_Chr5g0434591 47.917 96 48 2 412 505 352 447 2.91e-21 97.4
Msa0080820 MtrunA17_Chr5g0439251 54.444 90 39 2 414 501 376 465 6.64e-21 96.3
Msa0080820 MtrunA17_Chr6g0487261 38.739 111 65 2 408 515 357 467 9.32e-21 96.3
Msa0080820 MtrunA17_Chr4g0043511 41.000 100 58 1 418 516 418 517 1.21e-20 95.9
Msa0080820 MtrunA17_Chr4g0040911 50.526 95 45 2 420 512 37 131 1.74e-20 89.4
Msa0080820 MtrunA17_Chr4g0061401 52.941 85 38 2 419 502 23 106 2.93e-20 89.7
Msa0080820 MtrunA17_Chr1g0213651 46.591 88 46 1 416 502 272 359 3.39e-20 92.8
Msa0080820 MtrunA17_Chr3g0110371 40.984 122 70 2 397 516 338 459 3.54e-20 94.4
Msa0080820 MtrunA17_Chr5g0419121 50.000 92 43 2 417 505 19 110 5.85e-20 90.1
Msa0080820 MtrunA17_Chr4g0043151 50.000 88 42 2 420 505 21 108 2.14e-19 86.7
Msa0080820 MtrunA17_Chr4g0034901 46.018 113 52 4 403 507 353 464 3.24e-19 90.9
Msa0080820 MtrunA17_Chr8g0389741 36.441 118 73 2 411 526 359 476 4.36e-19 90.9
Msa0080820 MtrunA17_Chr4g0076141 47.727 88 44 2 414 499 7 94 5.08e-19 82.4
Msa0080820 MtrunA17_Chr2g0313891 46.392 97 49 3 408 502 17 112 7.52e-19 82.4
Msa0080820 MtrunA17_Chr1g0161011 40.517 116 67 2 418 531 359 474 1.04e-18 89.7
Msa0080820 MtrunA17_Chr4g0076131 46.154 91 47 2 414 502 22 112 1.13e-18 82.0
Msa0080820 MtrunA17_Chr8g0339191 39.216 102 55 3 405 504 364 460 1.13e-16 83.2
Msa0080820 MtrunA17_Chr3g0144531 43.119 109 57 3 403 507 352 459 1.56e-16 82.8
Msa0080820 MtrunA17_Chr7g0234821 42.478 113 60 5 395 504 350 460 3.83e-16 81.6
Msa0080820 MtrunA17_Chr7g0223961 39.216 102 61 1 400 500 345 446 1.20e-14 76.6
Msa0080820 MtrunA17_Chr2g0306311 35.849 106 58 5 418 518 397 497 4.64e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0080820 AT1G09460.1 69.048 126 36 2 386 509 101 225 6.37e-44 160
Msa0080820 AT2G30933.1 60.638 94 36 1 418 510 80 173 1.03e-33 129
Msa0080820 AT1G29380.3 65.556 90 30 1 420 508 148 237 2.70e-32 126
Msa0080820 AT1G29380.1 66.667 84 27 1 420 502 148 231 5.09e-31 124
Msa0080820 AT2G30933.2 44.615 130 40 2 368 496 61 159 1.63e-29 114
Msa0080820 AT2G30933.3 44.615 130 40 2 368 496 61 159 1.63e-29 114
Msa0080820 AT1G29380.4 67.949 78 24 1 420 496 148 225 3.47e-29 116
Msa0080820 AT1G29380.2 67.949 78 24 1 420 496 148 225 3.47e-29 116
Msa0080820 AT2G05790.1 54.310 116 48 3 395 505 356 471 5.10e-29 121
Msa0080820 AT1G11820.2 51.515 99 46 2 418 514 379 477 6.76e-26 112
Msa0080820 AT4G13600.1 44.094 127 66 3 420 542 61 186 1.27e-25 106
Msa0080820 AT4G05430.1 44.444 117 63 2 400 514 3 119 1.73e-25 102
Msa0080820 AT5G55180.1 53.684 95 42 2 413 505 365 459 2.65e-25 110
Msa0080820 AT4G05430.3 44.167 120 65 2 397 514 25 144 4.57e-25 102
Msa0080820 AT4G26830.1 35.751 193 110 6 322 505 267 454 3.11e-24 106
Msa0080820 AT4G26830.2 35.751 193 110 6 322 505 271 458 3.95e-24 106
Msa0080820 AT5G55180.2 56.977 86 35 2 413 496 365 450 5.14e-23 103
Msa0080820 AT3G55430.1 45.652 92 49 1 412 502 358 449 5.68e-23 102
Msa0080820 AT5G35740.1 52.747 91 40 3 415 502 25 115 1.68e-22 93.6
Msa0080820 AT4G34480.3 48.837 86 43 1 419 503 252 337 3.11e-22 99.8
Msa0080820 AT2G01630.1 39.535 129 74 3 418 544 358 484 5.55e-22 100
Msa0080820 AT4G34480.1 48.837 86 43 1 419 503 363 448 5.56e-22 100
Msa0080820 AT3G58100.1 48.936 94 46 2 420 511 41 134 8.17e-22 93.6
Msa0080820 AT1G18650.1 47.778 90 46 1 419 507 20 109 1.16e-21 93.2
Msa0080820 AT3G58100.2 47.959 98 49 2 416 511 113 210 1.57e-21 95.1
Msa0080820 AT1G11820.3 54.321 81 35 2 418 496 379 459 2.77e-21 97.8
Msa0080820 AT1G18650.2 47.191 89 46 1 419 506 20 108 8.03e-21 89.4
Msa0080820 AT4G05430.2 46.078 102 53 2 397 496 25 126 8.16e-21 89.0
Msa0080820 AT1G79480.2 50.000 84 41 1 420 502 310 393 1.44e-20 94.7
Msa0080820 AT1G79480.1 50.000 84 41 1 420 502 311 394 1.46e-20 94.7
Msa0080820 AT1G66250.1 39.474 114 66 3 418 529 366 478 1.80e-20 95.5
Msa0080820 AT2G16230.1 40.777 103 60 1 419 520 365 467 2.87e-20 95.1
Msa0080820 AT5G61130.1 47.674 86 44 1 419 503 20 105 7.93e-20 88.6
Msa0080820 AT5G67460.1 48.235 85 42 2 420 502 293 377 2.13e-19 91.3
Msa0080820 AT1G69295.1 56.338 71 29 2 419 488 20 89 2.49e-19 87.8
Msa0080820 AT1G69295.2 56.338 71 29 2 419 488 16 85 3.90e-19 87.0
Msa0080820 AT1G26450.1 38.312 154 67 6 421 550 22 171 5.47e-19 85.9
Msa0080820 AT5G56590.1 40.777 103 59 2 420 520 369 471 8.00e-19 90.5
Msa0080820 AT1G66870.2 42.529 87 48 2 418 502 51 137 8.47e-19 83.6
Msa0080820 AT5G63225.1 46.512 86 45 1 418 502 23 108 9.18e-19 82.8
Msa0080820 AT1G66870.1 42.529 87 48 2 418 502 24 110 1.00e-18 82.4
Msa0080820 AT5G67460.2 50.633 79 37 2 420 496 293 371 1.31e-18 88.6
Msa0080820 AT5G24318.3 36.697 109 62 2 398 505 217 319 1.82e-18 87.4
Msa0080820 AT5G24318.1 36.697 109 62 2 398 505 356 458 2.21e-18 89.0
Msa0080820 AT4G29360.2 45.977 87 45 2 418 502 399 485 2.30e-18 89.4
Msa0080820 AT4G29360.1 45.977 87 45 2 418 502 389 475 2.53e-18 89.0
Msa0080820 AT4G09462.1 41.758 91 52 1 415 504 24 114 3.59e-18 80.9
Msa0080820 AT3G13560.4 43.434 99 54 2 420 516 362 460 4.70e-18 88.2
Msa0080820 AT3G13560.1 43.434 99 54 2 420 516 362 460 4.70e-18 88.2
Msa0080820 AT3G13560.2 43.434 99 54 2 420 516 362 460 4.70e-18 88.2
Msa0080820 AT3G13560.5 43.434 99 54 2 420 516 362 460 4.70e-18 88.2
Msa0080820 AT3G13560.3 43.434 99 54 2 420 516 362 460 4.70e-18 88.2
Msa0080820 AT5G08000.2 44.262 122 66 2 417 536 37 158 5.53e-18 83.6
Msa0080820 AT4G09465.1 40.659 91 53 1 415 504 26 116 6.05e-18 80.5
Msa0080820 AT4G09464.1 40.659 91 53 1 415 504 26 116 7.86e-18 80.1
Msa0080820 AT4G09467.1 40.659 91 53 1 415 504 26 116 7.86e-18 80.1
Msa0080820 AT4G09090.1 43.023 86 48 1 420 504 31 116 1.52e-17 79.3
Msa0080820 AT1G66852.1 39.535 86 51 1 418 502 24 109 1.54e-17 79.0
Msa0080820 AT2G01630.3 48.148 81 40 2 418 496 358 438 1.54e-17 85.9
Msa0080820 AT4G09466.1 40.659 91 53 1 415 504 26 116 1.60e-17 79.3
Msa0080820 AT2G42930.1 44.872 78 43 0 420 497 40 117 1.97e-17 79.7
Msa0080820 AT3G23770.1 44.118 102 53 4 405 504 374 473 3.45e-17 85.1
Msa0080820 AT5G08000.1 47.674 86 44 1 419 503 20 105 5.34e-17 80.1
Msa0080820 AT1G78520.1 40.000 90 52 2 414 502 26 114 9.37e-17 77.0
Msa0080820 AT2G04910.2 43.182 88 48 2 415 500 10 97 1.12e-16 76.6
Msa0080820 AT5G63240.1 37.255 102 60 2 407 504 27 128 1.36e-16 77.0
Msa0080820 AT5G24318.2 37.000 100 56 2 398 496 356 449 5.24e-16 81.3
Msa0080820 AT1G66855.1 39.080 87 51 2 418 502 24 110 1.42e-15 73.6
Msa0080820 AT5G63230.1 37.363 91 56 1 413 502 19 109 2.81e-15 72.8
Msa0080820 AT5G63250.1 38.776 98 55 3 412 504 33 130 3.21e-15 73.2
Msa0080820 AT5G53600.1 40.698 86 49 2 418 502 27 111 1.21e-14 70.9
Msa0080820 AT4G16165.1 37.931 87 52 2 418 502 24 110 1.29e-14 70.9
Msa0080820 AT2G43670.1 38.298 94 55 3 410 502 26 117 2.63e-14 70.1
Msa0080820 AT2G39640.1 34.091 88 57 1 419 505 459 546 3.07e-14 76.3
Msa0080820 AT2G39640.1 36.458 96 54 2 406 500 351 440 7.55e-13 72.0
Msa0080820 AT2G04910.1 43.678 87 46 3 412 496 8 93 3.80e-14 70.1
Msa0080820 AT2G43660.2 41.667 96 52 4 409 502 26 119 7.15e-14 68.9
Msa0080820 AT2G43660.1 41.667 96 52 4 409 502 25 118 8.30e-14 68.9
Msa0080820 AT2G29735.1 40.860 93 52 2 413 502 24 116 1.68e-13 67.8
Msa0080820 AT3G07320.1 39.216 102 58 3 405 504 358 457 2.10e-13 73.2
Msa0080820 AT4G14080.1 37.255 102 62 2 405 504 374 475 2.83e-13 73.2
Msa0080820 AT1G13830.2 39.130 92 47 3 417 502 32 120 5.48e-13 68.9
Msa0080820 AT2G03505.3 36.667 90 54 2 419 505 20 109 7.26e-13 66.2
Msa0080820 AT1G13830.1 39.326 89 45 3 420 502 21 106 7.55e-13 68.2
Msa0080820 AT2G03505.4 36.667 90 54 2 419 505 20 109 7.62e-13 67.4
Msa0080820 AT2G03505.1 36.667 90 54 2 419 505 20 109 7.62e-13 67.4
Msa0080820 AT2G03505.2 36.667 90 54 2 419 505 20 109 7.62e-13 67.4
Msa0080820 AT5G53610.1 41.463 82 46 2 420 500 28 108 1.49e-12 65.1
Msa0080820 AT5G64790.1 38.144 97 56 4 418 512 367 461 3.34e-12 69.7

Find 172 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATCGCCATCAGTGCTTTC+TGG 0.163697 1_2:-59207252 Msa0080830:three_prime_UTR
AATATATTCATTAAATTATC+AGG 0.166823 1_2:+59207223 Msa0080820:three_prime_UTR
TCAGGTACAATGCCAGATTT+TGG 0.194586 1_2:+59206628 Msa0080820:CDS
CCAACATCTTCAGGCACTTT+TGG 0.216484 1_2:+59206458 Msa0080820:CDS
CAATATATTATGGTCATTAT+TGG 0.244640 1_2:+59206953 Msa0080820:three_prime_UTR
TCACTTTCATGCAGGTTCTT+TGG 0.248475 1_2:+59204732 Msa0080820:intron
TCTCTCTTAATTTCATGTTC+TGG 0.269637 1_2:+59204636 Msa0080820:CDS
TAGATTACTTTCATTTATAA+AGG 0.277901 1_2:+59205188 Msa0080820:CDS
TGAGTGGGAAGGAGAGAAAA+AGG 0.289037 1_2:-59204471 None:intergenic
TTAGGTTGTACAATATATTA+TGG 0.299298 1_2:+59206943 Msa0080820:three_prime_UTR
GGTGGAGCTGTTGGAATTGA+TGG 0.308099 1_2:-59206437 None:intergenic
CATTTCCACTTCAAGTCTTA+AGG 0.342871 1_2:+59205133 Msa0080820:CDS
AAGACTATACAGCCAGTAAA+AGG 0.355621 1_2:-59206709 None:intergenic
TTAGTGTGATTGATATATAC+AGG 0.373763 1_2:-59204538 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.377508 1_2:-59205840 None:intergenic
ATGATAGAAGATAGCATTGA+TGG 0.377602 1_2:+59205228 Msa0080820:CDS
TAGCAAATAACTTTAGATAA+AGG 0.378208 1_2:-59204497 None:intergenic
CCTCCAAAGTCGCAACTTGT+TGG 0.380624 1_2:-59206119 None:intergenic
GGTGTAATGGTTGGAGTTGT+TGG 0.382964 1_2:-59205744 None:intergenic
GGTGGAGGTGTTGTTAGATT+TGG 0.383669 1_2:-59206371 None:intergenic
GGCATTGCAGCTGGATTTGT+AGG 0.384777 1_2:-59205681 None:intergenic
TGTGGAAGGAACTGCTATAA+TGG 0.388341 1_2:-59205640 None:intergenic
AACTCAAGCAACCCAGCTTC+AGG 0.391337 1_2:+59206610 Msa0080820:CDS
GTCAGAGTTGTTATAATTTG+AGG 0.392142 1_2:+59207062 Msa0080820:three_prime_UTR
GCTCCAACAAGTTGCGACTT+TGG 0.392668 1_2:+59206116 Msa0080820:CDS
TACACAATACTCTCTGATAA+TGG 0.394136 1_2:+59204578 Msa0080820:exon
ATAACTGGTGTTGGATTTGT+AGG 0.394170 1_2:-59205597 None:intergenic
GCATGGTTCTGCAAAGTAAC+AGG 0.394899 1_2:-59206056 None:intergenic
ACGAAGAAACTTTGCTCTGC+TGG 0.394901 1_2:-59204801 None:intergenic
GAGCTTTCGTCACAGATCAT+AGG 0.396108 1_2:+59204833 Msa0080820:CDS
GGAGGTGTTGTTAGATTTGG+TGG 0.400113 1_2:-59206368 None:intergenic
CGAGTTACTTCTATTGAACT+TGG 0.401043 1_2:-59205369 None:intergenic
TTGGATTATGCTTGTGGAAA+TGG 0.401736 1_2:+59205990 Msa0080820:CDS
CAATGTAGTTCAGTGTAAAT+TGG 0.409911 1_2:+59207040 Msa0080820:three_prime_UTR
CACAAATGCTCCCTCTGCTC+AGG 0.410849 1_2:+59205911 Msa0080820:CDS
CTTCAATGAAAGAACTCAAA+AGG 0.413209 1_2:+59205472 Msa0080820:CDS
GTAGTTGAAGGAACTGTTAC+TGG 0.413784 1_2:-59205702 None:intergenic
GAGGGAGCATTTGTGTTTGA+AGG 0.413852 1_2:-59205903 None:intergenic
GTTGGAGTTGTTGGAGGTAA+AGG 0.413921 1_2:-59205735 None:intergenic
TTGAAGGAGGTGGCTGTTGA+GGG 0.417203 1_2:-59205887 None:intergenic
CATTACATGCTGCAGTGTTA+AGG 0.418204 1_2:+59206845 Msa0080820:three_prime_UTR
ACAGCTCCACCAACATCTTC+AGG 0.424830 1_2:+59206449 Msa0080820:CDS
ACCACTGGAACATTTCCTAA+TGG 0.424925 1_2:-59205861 None:intergenic
GGATAAGATGTTACTGGAGG+TGG 0.426667 1_2:-59205834 None:intergenic
GCCGATGTTGTGTTTACAAT+AGG 0.430103 1_2:-59206664 None:intergenic
ACTGTTACTGGCATTGCAGC+TGG 0.430772 1_2:-59205690 None:intergenic
TGATTGTTCTCAAATACAAC+AGG 0.433295 1_2:+59206016 Msa0080820:CDS
ACAACTCCTACAACAGTTCC+AGG 0.436358 1_2:+59205789 Msa0080820:CDS
ACATCTTATCCACCTCCATT+AGG 0.436623 1_2:+59205846 Msa0080820:CDS
CTTTCATGCAGGTTCTTTGG+TGG 0.439102 1_2:+59204735 Msa0080820:intron
GGAAATTGTGACAGGATTTG+TGG 0.439347 1_2:-59205658 None:intergenic
GGGCTATCAGACCCAAAATC+TGG 0.444861 1_2:-59206640 None:intergenic
CACCTATAGAGTTCAGAAAC+AGG 0.446988 1_2:+59206766 Msa0080820:exon
TTTCATGCAGGTTCTTTGGT+GGG 0.448012 1_2:+59204736 Msa0080820:intron
TTTGAAGGAGGTGGCTGTTG+AGG 0.448619 1_2:-59205888 None:intergenic
GGTGGAGCTGTTGACATTGT+TGG 0.450114 1_2:-59206416 None:intergenic
AGCTTCCCTTCAATCAGCAT+TGG 0.454325 1_2:+59205971 Msa0080820:CDS
GCACATGTGAAATGCTTTCA+AGG 0.454934 1_2:+59207305 Msa0080820:three_prime_UTR
AACCTGTTTCTGAACTCTAT+AGG 0.458686 1_2:-59206768 None:intergenic
GCATTTGTGTTTGAAGGAGG+TGG 0.463327 1_2:-59205897 None:intergenic
CTGAGGCTTGCAGTGACAAA+TGG 0.467298 1_2:-59206739 None:intergenic
AGTTGGTGTGTGGCGAAAAC+AGG 0.475765 1_2:+59205939 Msa0080820:CDS
GGCGCTATTGTCGGAATAGA+TGG 0.476552 1_2:-59206392 None:intergenic
AAGTCGCAACTTGTTGGAGC+TGG 0.478203 1_2:-59206113 None:intergenic
TCTGGCATTGTACCTGAAGC+TGG 0.478959 1_2:-59206622 None:intergenic
AACTTCCTTAAGACTTGAAG+TGG 0.484162 1_2:-59205138 None:intergenic
GAGGCAGAAAGAAGCTTCCT+TGG 0.484282 1_2:-59204601 None:intergenic
GGTGCACCTGGAACTGTTGT+AGG 0.485248 1_2:-59205795 None:intergenic
GTAGGGTAAACTGTGTTTGT+TGG 0.486254 1_2:-59205579 None:intergenic
GGAGGTGGATAAGATGTTAC+TGG 0.487201 1_2:-59205819 None:intergenic
AGAATCAGCATCCAATGCAT+TGG 0.490685 1_2:-59207192 None:intergenic
GGCTGTTGAGGGTTTACCAC+TGG 0.491135 1_2:-59205876 None:intergenic
CATAATCCAATGCTGATTGA+AGG 0.493090 1_2:-59205977 None:intergenic
GTTGATTGTGTAGGTGGTGT+TGG 0.494210 1_2:-59206329 None:intergenic
TTTGTAGGGGAAATTGTGAC+AGG 0.494725 1_2:-59205666 None:intergenic
TATCTAGAATATTTCTTCTG+TGG 0.496929 1_2:-59205545 None:intergenic
TATATAGAAGCACCTGTCAC+AGG 0.499776 1_2:-59207140 None:intergenic
AAAATATTCTAAGCAGCTCA+TGG 0.499869 1_2:-59206922 None:intergenic
AGTGTCTCCTCTCTCATGAT+AGG 0.500543 1_2:-59204764 None:intergenic
ATTGTTGGTGGCGCTATTGT+CGG 0.502128 1_2:-59206401 None:intergenic
GTATCTGAGGGTGTAATAAC+TGG 0.502389 1_2:-59205612 None:intergenic
GCATTGCAGCTGGATTTGTA+GGG 0.503176 1_2:-59205680 None:intergenic
CAGTGATCTTCACATAAGTA+AGG 0.508886 1_2:+59205092 Msa0080820:CDS
GATGTTACTGGTGGTGCACC+TGG 0.511282 1_2:-59205807 None:intergenic
GGACTATAAGTGCAGAAAGA+AGG 0.511738 1_2:+59206866 Msa0080820:three_prime_UTR
TTCAATGAAAGAACTCAAAA+GGG 0.511868 1_2:+59205473 Msa0080820:CDS
CTTGAGTTTAACACTGAAGG+TGG 0.514620 1_2:-59206595 None:intergenic
TCAGCATTGGATTATGCTTG+TGG 0.514748 1_2:+59205984 Msa0080820:CDS
CTCTTGATTGTAAGAACCAC+AGG 0.520516 1_2:-59205336 None:intergenic
GGAGCATTTGTGTTTGAAGG+AGG 0.521515 1_2:-59205900 None:intergenic
GAAGACTGGTGCCGGTGTAA+TGG 0.528319 1_2:-59205757 None:intergenic
GATGTTGTGTTTACAATAGG+AGG 0.530245 1_2:-59206661 None:intergenic
TACTAAGCACTCACTTCCAC+AGG 0.531203 1_2:-59205016 None:intergenic
TCCTTGCAAAGATGTTCCTG+TGG 0.532621 1_2:+59205320 Msa0080820:CDS
CCAACAAGTTGCGACTTTGG+AGG 0.533263 1_2:+59206119 Msa0080820:CDS
GAAGATGTTGGTGGAGCTGT+TGG 0.534788 1_2:-59206446 None:intergenic
GGAACAGCAGGTGTAGTTGA+AGG 0.536488 1_2:-59205714 None:intergenic
TTTAGATAAAGGAGATGAGT+GGG 0.537957 1_2:-59204486 None:intergenic
GAAAGAACTCAAAAGGGAAG+AGG 0.546037 1_2:+59205479 Msa0080820:CDS
GATAAAGGAGATGAGTGGGA+AGG 0.546644 1_2:-59204482 None:intergenic
CAACTCTGTCCCTGAGCAGA+GGG 0.550836 1_2:-59205921 None:intergenic
GGAACATTTCCTAATGGAGG+TGG 0.552963 1_2:-59205855 None:intergenic
GTTGGAGGTAAAGGAACAGC+AGG 0.553488 1_2:-59205726 None:intergenic
TCTTACAAACTCAAATGTGT+TGG 0.554054 1_2:+59204867 Msa0080820:CDS
AAAAGCATCGTTGCTGCCTG+TGG 0.556134 1_2:+59205000 Msa0080820:CDS
TGTAGCTTAATCCAATGCAT+TGG 0.557109 1_2:+59207181 Msa0080820:three_prime_UTR
AAAGCATTTCACATGTGCGC+AGG 0.560995 1_2:-59207300 Msa0080830:three_prime_UTR
ATATATTCATTAAATTATCA+GGG 0.562769 1_2:+59207224 Msa0080820:three_prime_UTR
GAGGGTGTAATAACTGGTGT+TGG 0.563477 1_2:-59205606 None:intergenic
ATAATCCAATGCTGATTGAA+GGG 0.564193 1_2:-59205976 None:intergenic
ACTGTTGTAGGAGTTGTAGC+TGG 0.566759 1_2:-59205783 None:intergenic
TTTAGCTCAGAAACTGCTGA+AGG 0.570280 1_2:-59204940 None:intergenic
CCTCTGCTCAGGGACAGAGT+TGG 0.572186 1_2:+59205922 Msa0080820:CDS
GGTGTTGTTAGATTTGGTGG+TGG 0.572595 1_2:-59206365 None:intergenic
TTGAGTTTAACACTGAAGGT+GGG 0.573230 1_2:-59206594 None:intergenic
TAACTGGTGTTGGATTTGTA+GGG 0.580532 1_2:-59205596 None:intergenic
TTGCTTGAGTTTAACACTGA+AGG 0.581994 1_2:-59206598 None:intergenic
TTAAGAGCATGCATAATCAA+AGG 0.583126 1_2:-59205051 None:intergenic
TTTCAGAGCTCGAGGGTGCG+AGG 0.590599 1_2:-59206686 None:intergenic
TATAATGGTTGGAGTATCTG+AGG 0.591925 1_2:-59205625 None:intergenic
GAAGGAACTGCTATAATGGT+TGG 0.591976 1_2:-59205636 None:intergenic
AAGATCTCTCTTCTAGACAA+AGG 0.594620 1_2:-59205513 None:intergenic
GGTGTTGGAGATGATATCAT+TGG 0.598845 1_2:-59206314 None:intergenic
AAGTAAGGAAGTGAAAATCT+CGG 0.601968 1_2:+59205107 Msa0080820:CDS
ATACTCTCTGATAATGGCCA+AGG 0.605015 1_2:+59204584 Msa0080820:exon
GTAATGGTTGGAGTTGTTGG+AGG 0.606112 1_2:-59205741 None:intergenic
ATCATGAGAGAGGAGACACT+TGG 0.607322 1_2:+59204767 Msa0080820:CDS
GTAAAAGGTTTCAGAGCTCG+AGG 0.607544 1_2:-59206694 None:intergenic
TGAGTTTAACACTGAAGGTG+GGG 0.608299 1_2:-59206593 None:intergenic
CTTTAGATAAAGGAGATGAG+TGG 0.608450 1_2:-59204487 None:intergenic
GAAGTTGAAGTTGATTGTGT+AGG 0.609053 1_2:-59206338 None:intergenic
TGATATCATTGGTGAAGTTG+AGG 0.609455 1_2:-59206303 None:intergenic
GGAGCTGTTGACATTGTTGG+TGG 0.610709 1_2:-59206413 None:intergenic
GTTGAAGTTGATTGTGTAGG+TGG 0.611559 1_2:-59206335 None:intergenic
ACAAATGCTCCCTCTGCTCA+GGG 0.611713 1_2:+59205912 Msa0080820:CDS
ATGTTGTGTTTACAATAGGA+GGG 0.618672 1_2:-59206660 None:intergenic
GTTGTAGCTGGATTGAAGAC+TGG 0.620005 1_2:-59205771 None:intergenic
GGTACTCTATACCCTGTGAC+AGG 0.620454 1_2:+59207128 Msa0080820:three_prime_UTR
ATTGTGACAGGATTTGTGGA+AGG 0.620979 1_2:-59205654 None:intergenic
GCTGGATTGAAGACTGGTGC+CGG 0.624496 1_2:-59205765 None:intergenic
AACAACTCCAACCATTACAC+CGG 0.624559 1_2:+59205746 Msa0080820:CDS
GGAGCTGTTGGAATTGATGG+TGG 0.624833 1_2:-59206434 None:intergenic
ACTGGTGCCGGTGTAATGGT+TGG 0.625957 1_2:-59205753 None:intergenic
CTGGCATTGTACCTGAAGCT+GGG 0.626526 1_2:-59206621 None:intergenic
TTAGCTCAGAAACTGCTGAA+GGG 0.631188 1_2:-59204939 None:intergenic
ACCACAGGAACATCTTTGCA+AGG 0.631919 1_2:-59205321 None:intergenic
AACTCTATAGGTGCATGCTG+AGG 0.632489 1_2:-59206756 None:intergenic
CATTGCAGCTGGATTTGTAG+GGG 0.635434 1_2:-59205679 None:intergenic
CCAACTCTGTCCCTGAGCAG+AGG 0.639664 1_2:-59205922 None:intergenic
GAACTCAAAAGGGAAGAGGA+AGG 0.639773 1_2:+59205483 Msa0080820:CDS
CCAAAAGTGCCTGAAGATGT+TGG 0.642640 1_2:-59206458 None:intergenic
ATTGTCGGAATAGATGGTGG+AGG 0.645107 1_2:-59206386 None:intergenic
TGTTGAAGGCAAAAGAAGCA+TGG 0.645483 1_2:-59206073 None:intergenic
ATATAGAAGCACCTGTCACA+GGG 0.657268 1_2:-59207139 None:intergenic
TCCATTAGGAAATGTTCCAG+TGG 0.658091 1_2:+59205860 Msa0080820:CDS
ACTGGAACATTTCCTAATGG+AGG 0.658231 1_2:-59205858 None:intergenic
GAGTAGTGTGATGATCCGAA+TGG 0.661571 1_2:-59206793 None:intergenic
GCTGATTGAAGGGAAGCTTG+TGG 0.661755 1_2:-59205966 None:intergenic
CATTGATGGAGAATTAAGTG+TGG 0.667086 1_2:+59205242 Msa0080820:CDS
TAAAAGGTTTCAGAGCTCGA+GGG 0.668012 1_2:-59206693 None:intergenic
TCCTATTGTAAACACAACAT+CGG 0.678848 1_2:+59206663 Msa0080820:CDS
AGAGAGAAGAGAGAAACATG+AGG 0.679970 1_2:-59204620 None:intergenic
CAGAAGCAGCAAGATTAGCG+CGG 0.682382 1_2:-59205293 None:intergenic
AAAGTGCCTGAAGATGTTGG+TGG 0.686382 1_2:-59206455 None:intergenic
GCTATTGTCGGAATAGATGG+TGG 0.692718 1_2:-59206389 None:intergenic
ATAATGGTTGGAGTATCTGA+GGG 0.698374 1_2:-59205624 None:intergenic
TCAGGGACAGAGTTGGTGTG+TGG 0.715688 1_2:+59205929 Msa0080820:CDS
GGTGGATAAGATGTTACTGG+AGG 0.717889 1_2:-59205837 None:intergenic
GATTGTTCTCAAATACAACA+GGG 0.719905 1_2:+59206017 Msa0080820:CDS
GACGACGATGAAGATGATGA+CGG 0.723295 1_2:-59206275 None:intergenic
CATTGGATTAAGCTACACGT+AGG 0.726032 1_2:-59207175 None:intergenic
GGTGGATAAGATGTTACTGG+TGG 0.740803 1_2:-59205816 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATTCATTAAATTATC+AGG + chr1_2:59207223-59207242 Msa0080820:three_prime_UTR 10.0%
!! ATATATTCATTAAATTATCA+GGG + chr1_2:59207224-59207243 Msa0080820:three_prime_UTR 10.0%
!!! AAATAATGAATTTTTCAACT+TGG - chr1_2:59204915-59204934 None:intergenic 15.0%
!! TAGATTACTTTCATTTATAA+AGG + chr1_2:59205188-59205207 Msa0080820:CDS 15.0%
!! TTAAATGTTAAAATCTTACT+TGG - chr1_2:59206170-59206189 None:intergenic 15.0%
!!! TATTGATGATCATTTTTAAT+AGG + chr1_2:59206233-59206252 Msa0080820:intron 15.0%
!! TAGCAAATAACTTTAGATAA+AGG - chr1_2:59204500-59204519 None:intergenic 20.0%
!!! TCTTTTTGCTTCTAAATATT+TGG - chr1_2:59205412-59205431 None:intergenic 20.0%
!! TGAAAGAAAGAAAAAAACAA+GGG - chr1_2:59206197-59206216 None:intergenic 20.0%
!! TTGAAAGAAAGAAAAAAACA+AGG - chr1_2:59206198-59206217 None:intergenic 20.0%
!!! TTAGGTTGTACAATATATTA+TGG + chr1_2:59206943-59206962 Msa0080820:three_prime_UTR 20.0%
!! CAATATATTATGGTCATTAT+TGG + chr1_2:59206953-59206972 Msa0080820:three_prime_UTR 20.0%
!!! TACATATATTTTGAGAACTT+AGG + chr1_2:59206981-59207000 Msa0080820:three_prime_UTR 20.0%
! TTAGTGTGATTGATATATAC+AGG - chr1_2:59204541-59204560 None:intergenic 25.0%
! ATATTATTTCACTTTCATGC+AGG + chr1_2:59204724-59204743 Msa0080820:intron 25.0%
!! ATGCTTTTGATGTTTAAATG+TGG - chr1_2:59204988-59205007 None:intergenic 25.0%
! TTCAATGAAAGAACTCAAAA+GGG + chr1_2:59205473-59205492 Msa0080820:CDS 25.0%
!! TATCTAGAATATTTCTTCTG+TGG - chr1_2:59205548-59205567 None:intergenic 25.0%
!!! TGATCATTTTTAATAGGTTC+TGG + chr1_2:59206239-59206258 Msa0080820:intron 25.0%
!! AGTTTTCAATTGTTTGTTCA+AGG + chr1_2:59207005-59207024 Msa0080820:three_prime_UTR 25.0%
!! TCATCATTTGACACAATTTT+TGG - chr1_2:59207108-59207127 None:intergenic 25.0%
! AAAAATTGTGTCAAATGATG+AGG + chr1_2:59207107-59207126 Msa0080820:three_prime_UTR 25.0%
! AAGGTGTCAAAAAAGAAAGA+GGG - chr1_2:59204455-59204474 None:intergenic 30.0%
!! AAAGGTGTCAAAAAAGAAAG+AGG - chr1_2:59204456-59204475 None:intergenic 30.0%
TTTAGATAAAGGAGATGAGT+GGG - chr1_2:59204489-59204508 None:intergenic 30.0%
TACACAATACTCTCTGATAA+TGG + chr1_2:59204578-59204597 Msa0080820:exon 30.0%
TCTCTCTTAATTTCATGTTC+TGG + chr1_2:59204636-59204655 Msa0080820:CDS 30.0%
TCTTACAAACTCAAATGTGT+TGG + chr1_2:59204867-59204886 Msa0080820:CDS 30.0%
! TTAAGAGCATGCATAATCAA+AGG - chr1_2:59205054-59205073 None:intergenic 30.0%
AAGTAAGGAAGTGAAAATCT+CGG + chr1_2:59205107-59205126 Msa0080820:CDS 30.0%
! ATGATAGAAGATAGCATTGA+TGG + chr1_2:59205228-59205247 Msa0080820:CDS 30.0%
CTTCAATGAAAGAACTCAAA+AGG + chr1_2:59205472-59205491 Msa0080820:CDS 30.0%
! ATAATCCAATGCTGATTGAA+GGG - chr1_2:59205979-59205998 None:intergenic 30.0%
TGATTGTTCTCAAATACAAC+AGG + chr1_2:59206016-59206035 Msa0080820:CDS 30.0%
GATTGTTCTCAAATACAACA+GGG + chr1_2:59206017-59206036 Msa0080820:CDS 30.0%
!! CTTTTGATAGTAACTGTTGA+AGG - chr1_2:59206090-59206109 None:intergenic 30.0%
CATGATTAGTACATGCTTAT+TGG - chr1_2:59206535-59206554 None:intergenic 30.0%
CAATAAGCATGTACTAATCA+TGG + chr1_2:59206533-59206552 Msa0080820:intron 30.0%
ATGTTGTGTTTACAATAGGA+GGG - chr1_2:59206663-59206682 None:intergenic 30.0%
TCCTATTGTAAACACAACAT+CGG + chr1_2:59206663-59206682 Msa0080820:CDS 30.0%
AAAATATTCTAAGCAGCTCA+TGG - chr1_2:59206925-59206944 None:intergenic 30.0%
! TGAGCTGCTTAGAATATTTT+AGG + chr1_2:59206925-59206944 Msa0080820:three_prime_UTR 30.0%
CAATGTAGTTCAGTGTAAAT+TGG + chr1_2:59207040-59207059 Msa0080820:three_prime_UTR 30.0%
GTCAGAGTTGTTATAATTTG+AGG + chr1_2:59207062-59207081 Msa0080820:three_prime_UTR 30.0%
CTTTAGATAAAGGAGATGAG+TGG - chr1_2:59204490-59204509 None:intergenic 35.0%
CAGTGATCTTCACATAAGTA+AGG + chr1_2:59205092-59205111 Msa0080820:CDS 35.0%
CATTTCCACTTCAAGTCTTA+AGG + chr1_2:59205133-59205152 Msa0080820:CDS 35.0%
AACTTCCTTAAGACTTGAAG+TGG - chr1_2:59205141-59205160 None:intergenic 35.0%
CATTGATGGAGAATTAAGTG+TGG + chr1_2:59205242-59205261 Msa0080820:CDS 35.0%
CGAGTTACTTCTATTGAACT+TGG - chr1_2:59205372-59205391 None:intergenic 35.0%
AAGATCTCTCTTCTAGACAA+AGG - chr1_2:59205516-59205535 None:intergenic 35.0%
! TAACTGGTGTTGGATTTGTA+GGG - chr1_2:59205599-59205618 None:intergenic 35.0%
! ATAACTGGTGTTGGATTTGT+AGG - chr1_2:59205600-59205619 None:intergenic 35.0%
! ATAATGGTTGGAGTATCTGA+GGG - chr1_2:59205627-59205646 None:intergenic 35.0%
! TATAATGGTTGGAGTATCTG+AGG - chr1_2:59205628-59205647 None:intergenic 35.0%
! CATAATCCAATGCTGATTGA+AGG - chr1_2:59205980-59205999 None:intergenic 35.0%
TTGGATTATGCTTGTGGAAA+TGG + chr1_2:59205990-59206009 Msa0080820:CDS 35.0%
TGATATCATTGGTGAAGTTG+AGG - chr1_2:59206306-59206325 None:intergenic 35.0%
! GAAGTTGAAGTTGATTGTGT+AGG - chr1_2:59206341-59206360 None:intergenic 35.0%
! AATGCTGTCTTTTGTAGCTA+TGG + chr1_2:59206568-59206587 Msa0080820:intron 35.0%
TTGAGTTTAACACTGAAGGT+GGG - chr1_2:59206597-59206616 None:intergenic 35.0%
TTGCTTGAGTTTAACACTGA+AGG - chr1_2:59206601-59206620 None:intergenic 35.0%
GATGTTGTGTTTACAATAGG+AGG - chr1_2:59206664-59206683 None:intergenic 35.0%
AAGACTATACAGCCAGTAAA+AGG - chr1_2:59206712-59206731 None:intergenic 35.0%
!! AACCTGTTTCTGAACTCTAT+AGG - chr1_2:59206771-59206790 None:intergenic 35.0%
! TCAGAAACAGGTTTTCCATT+CGG + chr1_2:59206778-59206797 Msa0080820:three_prime_UTR 35.0%
TGTAGCTTAATCCAATGCAT+TGG + chr1_2:59207181-59207200 Msa0080820:three_prime_UTR 35.0%
! GGTTAAAAAGTCAAGGTGTT+TGG - chr1_2:59207282-59207301 Msa0080830:three_prime_UTR 35.0%
!! ATACTCTCTGATAATGGCCA+AGG + chr1_2:59204584-59204603 Msa0080820:exon 40.0%
AGAGAGAAGAGAGAAACATG+AGG - chr1_2:59204623-59204642 None:intergenic 40.0%
TCACTTTCATGCAGGTTCTT+TGG + chr1_2:59204732-59204751 Msa0080820:intron 40.0%
TTTCATGCAGGTTCTTTGGT+GGG + chr1_2:59204736-59204755 Msa0080820:intron 40.0%
! GGTTTTTCCTATCATGAGAG+AGG + chr1_2:59204757-59204776 Msa0080820:CDS 40.0%
TTAGCTCAGAAACTGCTGAA+GGG - chr1_2:59204942-59204961 None:intergenic 40.0%
! TTTAGCTCAGAAACTGCTGA+AGG - chr1_2:59204943-59204962 None:intergenic 40.0%
CTCTTGATTGTAAGAACCAC+AGG - chr1_2:59205339-59205358 None:intergenic 40.0%
GAAAGAACTCAAAAGGGAAG+AGG + chr1_2:59205479-59205498 Msa0080820:CDS 40.0%
GTAGGGTAAACTGTGTTTGT+TGG - chr1_2:59205582-59205601 None:intergenic 40.0%
! GTATCTGAGGGTGTAATAAC+TGG - chr1_2:59205615-59205634 None:intergenic 40.0%
GAAGGAACTGCTATAATGGT+TGG - chr1_2:59205639-59205658 None:intergenic 40.0%
TGTGGAAGGAACTGCTATAA+TGG - chr1_2:59205643-59205662 None:intergenic 40.0%
ATTGTGACAGGATTTGTGGA+AGG - chr1_2:59205657-59205676 None:intergenic 40.0%
GGAAATTGTGACAGGATTTG+TGG - chr1_2:59205661-59205680 None:intergenic 40.0%
TTTGTAGGGGAAATTGTGAC+AGG - chr1_2:59205669-59205688 None:intergenic 40.0%
GTAGTTGAAGGAACTGTTAC+TGG - chr1_2:59205705-59205724 None:intergenic 40.0%
AACAACTCCAACCATTACAC+CGG + chr1_2:59205746-59205765 Msa0080820:CDS 40.0%
ACATCTTATCCACCTCCATT+AGG + chr1_2:59205846-59205865 Msa0080820:CDS 40.0%
ACTGGAACATTTCCTAATGG+AGG - chr1_2:59205861-59205880 None:intergenic 40.0%
TCCATTAGGAAATGTTCCAG+TGG + chr1_2:59205860-59205879 Msa0080820:CDS 40.0%
ACCACTGGAACATTTCCTAA+TGG - chr1_2:59205864-59205883 None:intergenic 40.0%
! TCAGCATTGGATTATGCTTG+TGG + chr1_2:59205984-59206003 Msa0080820:CDS 40.0%
TGTTGAAGGCAAAAGAAGCA+TGG - chr1_2:59206076-59206095 None:intergenic 40.0%
! GGTGTTGGAGATGATATCAT+TGG - chr1_2:59206317-59206336 None:intergenic 40.0%
! GTTGAAGTTGATTGTGTAGG+TGG - chr1_2:59206338-59206357 None:intergenic 40.0%
! CAACATCTTCAGGCACTTTT+GGG + chr1_2:59206459-59206478 Msa0080820:CDS 40.0%
TGAGTTTAACACTGAAGGTG+GGG - chr1_2:59206596-59206615 None:intergenic 40.0%
CTTGAGTTTAACACTGAAGG+TGG - chr1_2:59206598-59206617 None:intergenic 40.0%
! TCAGGTACAATGCCAGATTT+TGG + chr1_2:59206628-59206647 Msa0080820:CDS 40.0%
! CAGGTACAATGCCAGATTTT+GGG + chr1_2:59206629-59206648 Msa0080820:CDS 40.0%
GCCGATGTTGTGTTTACAAT+AGG - chr1_2:59206667-59206686 None:intergenic 40.0%
TAAAAGGTTTCAGAGCTCGA+GGG - chr1_2:59206696-59206715 None:intergenic 40.0%
CACCTATAGAGTTCAGAAAC+AGG + chr1_2:59206766-59206785 Msa0080820:exon 40.0%
CATTACATGCTGCAGTGTTA+AGG + chr1_2:59206845-59206864 Msa0080820:three_prime_UTR 40.0%
GGACTATAAGTGCAGAAAGA+AGG + chr1_2:59206866-59206885 Msa0080820:three_prime_UTR 40.0%
!! ATATAGAAGCACCTGTCACA+GGG - chr1_2:59207142-59207161 None:intergenic 40.0%
!! TATATAGAAGCACCTGTCAC+AGG - chr1_2:59207143-59207162 None:intergenic 40.0%
CATTGGATTAAGCTACACGT+AGG - chr1_2:59207178-59207197 None:intergenic 40.0%
AGAATCAGCATCCAATGCAT+TGG - chr1_2:59207195-59207214 None:intergenic 40.0%
!! GAAAAACCAGAAAGCACTGA+TGG + chr1_2:59207246-59207265 Msa0080820:three_prime_UTR 40.0%
GCACATGTGAAATGCTTTCA+AGG + chr1_2:59207305-59207324 Msa0080820:three_prime_UTR 40.0%
TGAGTGGGAAGGAGAGAAAA+AGG - chr1_2:59204474-59204493 None:intergenic 45.0%
GATAAAGGAGATGAGTGGGA+AGG - chr1_2:59204485-59204504 None:intergenic 45.0%
CTTTCATGCAGGTTCTTTGG+TGG + chr1_2:59204735-59204754 Msa0080820:intron 45.0%
AGTGTCTCCTCTCTCATGAT+AGG - chr1_2:59204767-59204786 None:intergenic 45.0%
ATCATGAGAGAGGAGACACT+TGG + chr1_2:59204767-59204786 Msa0080820:CDS 45.0%
ACGAAGAAACTTTGCTCTGC+TGG - chr1_2:59204804-59204823 None:intergenic 45.0%
GAGCTTTCGTCACAGATCAT+AGG + chr1_2:59204833-59204852 Msa0080820:CDS 45.0%
TACTAAGCACTCACTTCCAC+AGG - chr1_2:59205019-59205038 None:intergenic 45.0%
TCCTTGCAAAGATGTTCCTG+TGG + chr1_2:59205320-59205339 Msa0080820:CDS 45.0%
ACCACAGGAACATCTTTGCA+AGG - chr1_2:59205324-59205343 None:intergenic 45.0%
GAACTCAAAAGGGAAGAGGA+AGG + chr1_2:59205483-59205502 Msa0080820:CDS 45.0%
! GAGGGTGTAATAACTGGTGT+TGG - chr1_2:59205609-59205628 None:intergenic 45.0%
CATTGCAGCTGGATTTGTAG+GGG - chr1_2:59205682-59205701 None:intergenic 45.0%
GCATTGCAGCTGGATTTGTA+GGG - chr1_2:59205683-59205702 None:intergenic 45.0%
GTTGGAGTTGTTGGAGGTAA+AGG - chr1_2:59205738-59205757 None:intergenic 45.0%
GTAATGGTTGGAGTTGTTGG+AGG - chr1_2:59205744-59205763 None:intergenic 45.0%
GGTGTAATGGTTGGAGTTGT+TGG - chr1_2:59205747-59205766 None:intergenic 45.0%
GTTGTAGCTGGATTGAAGAC+TGG - chr1_2:59205774-59205793 None:intergenic 45.0%
ACTGTTGTAGGAGTTGTAGC+TGG - chr1_2:59205786-59205805 None:intergenic 45.0%
ACAACTCCTACAACAGTTCC+AGG + chr1_2:59205789-59205808 Msa0080820:CDS 45.0%
GGTGGATAAGATGTTACTGG+TGG - chr1_2:59205819-59205838 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG - chr1_2:59205822-59205841 None:intergenic 45.0%
GGATAAGATGTTACTGGAGG+TGG - chr1_2:59205837-59205856 None:intergenic 45.0%
GGTGGATAAGATGTTACTGG+AGG - chr1_2:59205819-59205838 None:intergenic 45.0%
GGAGGTGGATAAGATGTTAC+TGG - chr1_2:59205822-59205841 None:intergenic 45.0%
GGAACATTTCCTAATGGAGG+TGG - chr1_2:59205858-59205877 None:intergenic 45.0%
GCATTTGTGTTTGAAGGAGG+TGG - chr1_2:59205900-59205919 None:intergenic 45.0%
! GGAGCATTTGTGTTTGAAGG+AGG - chr1_2:59205903-59205922 None:intergenic 45.0%
!! GAGGGAGCATTTGTGTTTGA+AGG - chr1_2:59205906-59205925 None:intergenic 45.0%
AGCTTCCCTTCAATCAGCAT+TGG + chr1_2:59205971-59205990 Msa0080820:CDS 45.0%
! GCATGGTTCTGCAAAGTAAC+AGG - chr1_2:59206059-59206078 None:intergenic 45.0%
GACGACGATGAAGATGATGA+CGG - chr1_2:59206278-59206297 None:intergenic 45.0%
GTTGATTGTGTAGGTGGTGT+TGG - chr1_2:59206332-59206351 None:intergenic 45.0%
!! GGTGTTGTTAGATTTGGTGG+TGG - chr1_2:59206368-59206387 None:intergenic 45.0%
!! GGAGGTGTTGTTAGATTTGG+TGG - chr1_2:59206371-59206390 None:intergenic 45.0%
!! GGTGGAGGTGTTGTTAGATT+TGG - chr1_2:59206374-59206393 None:intergenic 45.0%
! ATTGTCGGAATAGATGGTGG+AGG - chr1_2:59206389-59206408 None:intergenic 45.0%
GCTATTGTCGGAATAGATGG+TGG - chr1_2:59206392-59206411 None:intergenic 45.0%
!! ATTGTTGGTGGCGCTATTGT+CGG - chr1_2:59206404-59206423 None:intergenic 45.0%
! AAAGTGCCTGAAGATGTTGG+TGG - chr1_2:59206458-59206477 None:intergenic 45.0%
!! CCAAAAGTGCCTGAAGATGT+TGG - chr1_2:59206461-59206480 None:intergenic 45.0%
! CCAACATCTTCAGGCACTTT+TGG + chr1_2:59206458-59206477 Msa0080820:CDS 45.0%
GTAAAAGGTTTCAGAGCTCG+AGG - chr1_2:59206697-59206716 None:intergenic 45.0%
!!! CGAGCTCTGAAACCTTTTAC+TGG + chr1_2:59206697-59206716 Msa0080820:CDS 45.0%
AACTCTATAGGTGCATGCTG+AGG - chr1_2:59206759-59206778 None:intergenic 45.0%
GAGTAGTGTGATGATCCGAA+TGG - chr1_2:59206796-59206815 None:intergenic 45.0%
GTGCGCAGGTTAAAAAGTCA+AGG - chr1_2:59207289-59207308 Msa0080830:three_prime_UTR 45.0%
! AAAGCATTTCACATGTGCGC+AGG - chr1_2:59207303-59207322 Msa0080830:three_prime_UTR 45.0%
GAGGCAGAAAGAAGCTTCCT+TGG - chr1_2:59204604-59204623 None:intergenic 50.0%
!! AAAAGCATCGTTGCTGCCTG+TGG + chr1_2:59205000-59205019 Msa0080820:CDS 50.0%
CAGAAGCAGCAAGATTAGCG+CGG - chr1_2:59205296-59205315 None:intergenic 50.0%
GGCATTGCAGCTGGATTTGT+AGG - chr1_2:59205684-59205703 None:intergenic 50.0%
ACTGTTACTGGCATTGCAGC+TGG - chr1_2:59205693-59205712 None:intergenic 50.0%
GGAACAGCAGGTGTAGTTGA+AGG - chr1_2:59205717-59205736 None:intergenic 50.0%
GTTGGAGGTAAAGGAACAGC+AGG - chr1_2:59205729-59205748 None:intergenic 50.0%
TTGAAGGAGGTGGCTGTTGA+GGG - chr1_2:59205890-59205909 None:intergenic 50.0%
TTTGAAGGAGGTGGCTGTTG+AGG - chr1_2:59205891-59205910 None:intergenic 50.0%
ACAAATGCTCCCTCTGCTCA+GGG + chr1_2:59205912-59205931 Msa0080820:CDS 50.0%
! AGTTGGTGTGTGGCGAAAAC+AGG + chr1_2:59205939-59205958 Msa0080820:CDS 50.0%
GCTGATTGAAGGGAAGCTTG+TGG - chr1_2:59205969-59205988 None:intergenic 50.0%
!! AAGTCGCAACTTGTTGGAGC+TGG - chr1_2:59206116-59206135 None:intergenic 50.0%
GCTCCAACAAGTTGCGACTT+TGG + chr1_2:59206116-59206135 Msa0080820:CDS 50.0%
CCTCCAAAGTCGCAACTTGT+TGG - chr1_2:59206122-59206141 None:intergenic 50.0%
CCAACAAGTTGCGACTTTGG+AGG + chr1_2:59206119-59206138 Msa0080820:CDS 50.0%
GGCGCTATTGTCGGAATAGA+TGG - chr1_2:59206395-59206414 None:intergenic 50.0%
GGAGCTGTTGACATTGTTGG+TGG - chr1_2:59206416-59206435 None:intergenic 50.0%
GGTGGAGCTGTTGACATTGT+TGG - chr1_2:59206419-59206438 None:intergenic 50.0%
GGAGCTGTTGGAATTGATGG+TGG - chr1_2:59206437-59206456 None:intergenic 50.0%
GGTGGAGCTGTTGGAATTGA+TGG - chr1_2:59206440-59206459 None:intergenic 50.0%
! GAAGATGTTGGTGGAGCTGT+TGG - chr1_2:59206449-59206468 None:intergenic 50.0%
ACAGCTCCACCAACATCTTC+AGG + chr1_2:59206449-59206468 Msa0080820:CDS 50.0%
!! GGCACTTTTGGGTAAGTGAG+AGG + chr1_2:59206470-59206489 Msa0080820:intron 50.0%
AACTCAAGCAACCCAGCTTC+AGG + chr1_2:59206610-59206629 Msa0080820:CDS 50.0%
CTGGCATTGTACCTGAAGCT+GGG - chr1_2:59206624-59206643 None:intergenic 50.0%
TCTGGCATTGTACCTGAAGC+TGG - chr1_2:59206625-59206644 None:intergenic 50.0%
GGGCTATCAGACCCAAAATC+TGG - chr1_2:59206643-59206662 None:intergenic 50.0%
CTGAGGCTTGCAGTGACAAA+TGG - chr1_2:59206742-59206761 None:intergenic 50.0%
GGTACTCTATACCCTGTGAC+AGG + chr1_2:59207128-59207147 Msa0080820:three_prime_UTR 50.0%
TGATCGCCATCAGTGCTTTC+TGG - chr1_2:59207255-59207274 Msa0080830:three_prime_UTR 50.0%
!! ACTGGTGCCGGTGTAATGGT+TGG - chr1_2:59205756-59205775 None:intergenic 55.0%
!! GAAGACTGGTGCCGGTGTAA+TGG - chr1_2:59205760-59205779 None:intergenic 55.0%
GCTGGATTGAAGACTGGTGC+CGG - chr1_2:59205768-59205787 None:intergenic 55.0%
GGTGCACCTGGAACTGTTGT+AGG - chr1_2:59205798-59205817 None:intergenic 55.0%
! GATGTTACTGGTGGTGCACC+TGG - chr1_2:59205810-59205829 None:intergenic 55.0%
GGCTGTTGAGGGTTTACCAC+TGG - chr1_2:59205879-59205898 None:intergenic 55.0%
CACAAATGCTCCCTCTGCTC+AGG + chr1_2:59205911-59205930 Msa0080820:CDS 55.0%
CAACTCTGTCCCTGAGCAGA+GGG - chr1_2:59205924-59205943 None:intergenic 55.0%
!! TCAGGGACAGAGTTGGTGTG+TGG + chr1_2:59205929-59205948 Msa0080820:CDS 55.0%
CCAACTCTGTCCCTGAGCAG+AGG - chr1_2:59205925-59205944 None:intergenic 60.0%
CCTCTGCTCAGGGACAGAGT+TGG + chr1_2:59205922-59205941 Msa0080820:CDS 60.0%
! TTTCAGAGCTCGAGGGTGCG+AGG - chr1_2:59206689-59206708 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 59204447 59207342 59204447 ID=Msa0080820;Name=Msa0080820
chr1_2 mRNA 59204447 59207342 59204447 ID=Msa0080820-mRNA-1;Parent=Msa0080820;Name=Msa0080820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=150|1|1|1|0.33|0.25|4|568|633
chr1_2 exon 59204447 59204657 59204447 ID=Msa0080820-mRNA-1:exon:19143;Parent=Msa0080820-mRNA-1
chr1_2 exon 59204746 59206170 59204746 ID=Msa0080820-mRNA-1:exon:19144;Parent=Msa0080820-mRNA-1
chr1_2 exon 59206255 59206480 59206255 ID=Msa0080820-mRNA-1:exon:19145;Parent=Msa0080820-mRNA-1
chr1_2 exon 59206585 59207342 59206585 ID=Msa0080820-mRNA-1:exon:19146;Parent=Msa0080820-mRNA-1
chr1_2 five_prime_UTR 59204447 59204596 59204447 ID=Msa0080820-mRNA-1:five_prime_utr;Parent=Msa0080820-mRNA-1
chr1_2 CDS 59204597 59204657 59204597 ID=Msa0080820-mRNA-1:cds;Parent=Msa0080820-mRNA-1
chr1_2 CDS 59204746 59206170 59204746 ID=Msa0080820-mRNA-1:cds;Parent=Msa0080820-mRNA-1
chr1_2 CDS 59206255 59206480 59206255 ID=Msa0080820-mRNA-1:cds;Parent=Msa0080820-mRNA-1
chr1_2 CDS 59206585 59206774 59206585 ID=Msa0080820-mRNA-1:cds;Parent=Msa0080820-mRNA-1
chr1_2 three_prime_UTR 59206775 59207342 59206775 ID=Msa0080820-mRNA-1:three_prime_utr;Parent=Msa0080820-mRNA-1
Gene Sequence

>Msa0080820

ATGGCCAAGGAAGCTTCTTTCTGCCTCATGTTTCTCTCTTCTCTCTTAATTTCATGTTCTGGTTCTTTGGTGGGTTTTTCCTATCATGAGAGAGGAGACACTTGGACATCATTACTCCAGCAGAGCAAAGTTTCTTCGTCTCAGATTCGAGCTTTCGTCACAGATCATAGGATTTTGAGCACTCTTACAAACTCAAATGTGTTGGTTGATCTCTACTTAAACAAAAGCCAAGTTGAAAAATTCATTATTTCAAACCCTTCAGCAGTTTCTGAGCTAAAAGCTCAACTAGTGAACTTTCTTCCACATTTAAACATCAAAAGCATCGTTGCTGCCTGTGGAAGTGAGTGCTTAGTACAAAATGAGATGCCTTTGATTATGCATGCTCTTAAATCAATTCATTCAATTCTCAGTGATCTTCACATAAGTAAGGAAGTGAAAATCTCGGTCGCATTTCCACTTCAAGTCTTAAGGAAGTTGAATGCATCACAACAACACGAAATTCGTAGATTACTTTCATTTATAAAGGAAACGAAATCGTTTGTAATGATAGAAGATAGCATTGATGGAGAATTAAGTGTGGATGATCATTTTGTTCAAACAATTATCAACCGCGCTAATCTTGCTGCTTCTGTTCTTCCTTGCAAAGATGTTCCTGTGGTTCTTACAATCAAGAGTTCAGTTATACCAAGTTCAATAGAAGTAACTCGATTTAGTAAAAGAGTATCCAAATATTTAGAAGCAAAAAGAATAGCTGCATTATATGTAGAACTGCATACAACAGAAGATTCTTCAATGAAAGAACTCAAAAGGGAAGAGGAAGGTATGTTTCCTTTGTCTAGAAGAGAGATCTTAAGCAAATTCCACAGAAGAAATATTCTAGATAACACAAACAGTCCAACAAACACAGTTTACCCTACAAATCCAACACCAGTTATTACACCCTCAGATACTCCAACCATTATAGCAGTTCCTTCCACAAATCCTGTCACAATTTCCCCTACAAATCCAGCTGCAATGCCAGTAACAGTTCCTTCAACTACACCTGCTGTTCCTTTACCTCCAACAACTCCAACCATTACACCGGCACCAGTCTTCAATCCAGCTACAACTCCTACAACAGTTCCAGGTGCACCACCAGTAACATCTTATCCACCTCCAGTAACATCTTATCCACCTCCATTAGGAAATGTTCCAGTGGTAAACCCTCAACAGCCACCTCCTTCAAACACAAATGCTCCCTCTGCTCAGGGACAGAGTTGGTGTGTGGCGAAAACAGGTGCTCCACAAGCTTCCCTTCAATCAGCATTGGATTATGCTTGTGGAAATGGTGCTGATTGTTCTCAAATACAACAGGGTGCAAGTTGTTACAGTCCTGTTACTTTGCAGAACCATGCTTCTTTTGCCTTCAACAGTTACTATCAAAAGAAGCCAGCTCCAACAAGTTGCGACTTTGGAGGCGCTGCTATGTTAGTTAGCTCAAATCCAAGTTCTGGTTCTTGCATTTATCCGTCATCATCTTCATCGTCGTCGTCATCCTCAACTTCACCAATGATATCATCTCCAACACCACCTACACAATCAACTTCAACTTCAATACCACCACCAAATCTAACAACACCTCCACCATCTATTCCGACAATAGCGCCACCAACAATGTCAACAGCTCCACCATCAATTCCAACAGCTCCACCAACATCTTCAGGCACTTTTGGCTATGGTACCCCACCTTCAGTGTTAAACTCAAGCAACCCAGCTTCAGGTACAATGCCAGATTTTGGGTCTGATAGCCCTCCTATTGTAAACACAACATCGGCCTCGCACCCTCGAGCTCTGAAACCTTTTACTGGCTGTATAGTCTTGATGATTCCATTTGTCACTGCAAGCCTCAGCATGCACCTATAG
Protein sequence

>Msa0080820

MAKEASFCLMFLSSLLISCSGSLVGFSYHERGDTWTSLLQQSKVSSSQIRAFVTDHRILSTLTNSNVLVDLYLNKSQVEKFIISNPSAVSELKAQLVNFLPHLNIKSIVAACGSECLVQNEMPLIMHALKSIHSILSDLHISKEVKISVAFPLQVLRKLNASQQHEIRRLLSFIKETKSFVMIEDSIDGELSVDDHFVQTIINRANLAASVLPCKDVPVVLTIKSSVIPSSIEVTRFSKRVSKYLEAKRIAALYVELHTTEDSSMKELKREEEGMFPLSRREILSKFHRRNILDNTNSPTNTVYPTNPTPVITPSDTPTIIAVPSTNPVTISPTNPAAMPVTVPSTTPAVPLPPTTPTITPAPVFNPATTPTTVPGAPPVTSYPPPVTSYPPPLGNVPVVNPQQPPPSNTNAPSAQGQSWCVAKTGAPQASLQSALDYACGNGADCSQIQQGASCYSPVTLQNHASFAFNSYYQKKPAPTSCDFGGAAMLVSSNPSSGSCIYPSSSSSSSSSSTSPMISSPTPPTQSTSTSIPPPNLTTPPPSIPTIAPPTMSTAPPSIPTAPPTSSGTFGYGTPPSVLNSSNPASGTMPDFGSDSPPIVNTTSASHPRALKPFTGCIVLMIPFVTASLSMHL*