AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0342840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0342840 MtrunA17_Chr7g0271411 90.323 310 25 2 1 310 1 305 9.68e-164 457
Msa0342840 MtrunA17_Chr1g0193131 56.388 227 64 10 42 240 279 498 2.85e-49 173
Msa0342840 MtrunA17_Chr5g0443331 57.037 135 55 2 137 271 86 217 2.20e-38 137
Msa0342840 MtrunA17_Chr3g0096451 63.551 107 36 1 139 245 93 196 2.92e-38 136
Msa0342840 MtrunA17_Chr2g0304061 62.069 87 32 1 161 247 158 243 1.20e-31 120
Msa0342840 MtrunA17_Chr8g0381081 46.903 113 52 3 139 246 34 143 6.44e-28 106
Msa0342840 MtrunA17_Chr1g0177171 40.667 150 80 4 102 246 347 492 1.41e-26 108
Msa0342840 MtrunA17_Chr4g0040911 49.153 118 47 3 161 275 37 144 2.52e-26 102
Msa0342840 MtrunA17_Chr8g0378621 37.209 172 90 4 136 302 339 497 1.25e-25 106
Msa0342840 MtrunA17_Chr8g0378361 51.042 96 45 2 159 253 28 122 1.34e-25 100
Msa0342840 MtrunA17_Chr5g0401301 41.667 144 72 4 105 244 279 414 1.77e-25 105
Msa0342840 MtrunA17_Chr4g0075031 51.613 93 44 1 154 245 375 467 5.53e-25 104
Msa0342840 MtrunA17_Chr8g0378371 54.545 88 37 2 160 247 32 116 4.25e-24 94.7
Msa0342840 MtrunA17_Chr3g0121271 39.695 131 68 3 148 277 349 469 9.87e-23 98.2
Msa0342840 MtrunA17_Chr8g0380231 59.155 71 29 0 168 238 24 94 4.93e-22 88.6
Msa0342840 MtrunA17_Chr8g0378381 50.562 89 38 3 160 245 31 116 5.14e-22 89.4
Msa0342840 MtrunA17_Chr1g0154991 47.706 109 46 5 147 252 8 108 7.16e-22 88.6
Msa0342840 MtrunA17_Chr5g0447651 49.412 85 42 1 161 244 30 114 1.63e-21 87.8
Msa0342840 MtrunA17_Chr5g0434591 42.056 107 61 1 154 259 353 459 1.70e-21 94.4
Msa0342840 MtrunA17_Chr5g0439251 47.253 91 47 1 154 243 375 465 4.36e-21 93.2
Msa0342840 MtrunA17_Chr3g0110371 43.299 97 54 1 154 249 354 450 5.37e-21 93.2
Msa0342840 MtrunA17_Chr4g0034901 43.860 114 54 3 143 248 352 463 2.32e-20 90.9
Msa0342840 MtrunA17_Chr4g0043511 44.340 106 57 2 160 264 419 523 3.25e-20 90.9
Msa0342840 MtrunA17_Chr3g0120401 45.977 87 45 2 161 246 157 242 3.60e-20 87.8
Msa0342840 MtrunA17_Chr5g0419121 47.368 95 46 3 156 247 17 110 5.43e-20 87.4
Msa0342840 MtrunA17_Chr8g0378401 50.602 83 35 3 160 239 31 110 1.87e-19 82.4
Msa0342840 MtrunA17_Chr1g0161011 40.909 110 59 2 154 262 354 458 2.41e-19 88.2
Msa0342840 MtrunA17_Chr4g0076131 41.379 87 50 1 160 245 27 113 4.54e-19 81.3
Msa0342840 MtrunA17_Chr4g0076141 44.304 79 43 1 161 238 13 91 7.10e-19 80.1
Msa0342840 MtrunA17_Chr6g0487261 40.541 111 61 4 136 244 347 454 2.72e-18 85.1
Msa0342840 MtrunA17_Chr4g0043151 47.253 91 45 3 161 249 21 110 3.00e-18 81.3
Msa0342840 MtrunA17_Chr1g0213651 44.706 85 45 2 161 244 276 359 4.66e-18 83.6
Msa0342840 MtrunA17_Chr4g0061401 44.944 89 46 3 157 244 20 106 7.37e-18 80.9
Msa0342840 MtrunA17_Chr8g0389741 33.099 142 84 3 136 271 348 484 2.22e-17 82.4
Msa0342840 MtrunA17_Chr2g0313891 44.186 86 45 2 160 244 29 112 1.85e-16 73.9
Msa0342840 MtrunA17_Chr7g0234821 38.983 118 67 3 133 248 348 462 2.90e-16 79.0
Msa0342840 MtrunA17_Chr3g0144531 39.815 108 59 3 144 248 354 458 2.95e-16 79.0
Msa0342840 MtrunA17_Chr8g0339191 36.082 97 61 1 152 247 365 461 3.91e-16 78.6
Msa0342840 MtrunA17_Chr2g0306311 40.816 98 53 4 160 255 398 492 1.48e-13 70.9
Msa0342840 MtrunA17_Chr7g0223961 41.379 87 45 3 159 242 363 446 1.77e-13 70.5
Msa0342840 MtrunA17_Chr3g0129341 35.714 112 62 5 141 245 348 456 9.73e-13 68.6
Msa0342840 MtrunA17_Chr4g0062771 34.483 116 71 4 160 273 373 485 2.31e-12 67.4
Msa0342840 MtrunA17_Chr4g0046311 36.905 84 52 1 157 239 369 452 1.19e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0342840 AT1G09460.1 59.375 128 48 2 121 248 99 222 2.64e-37 135
Msa0342840 AT2G30933.1 61.798 89 34 0 160 248 81 169 8.40e-34 124
Msa0342840 AT1G29380.3 58.889 90 37 0 160 249 147 236 1.18e-32 122
Msa0342840 AT1G29380.1 58.824 85 35 0 160 244 147 231 2.62e-31 119
Msa0342840 AT2G30933.2 62.025 79 30 0 160 238 81 159 2.22e-30 112
Msa0342840 AT2G30933.3 62.025 79 30 0 160 238 81 159 2.22e-30 112
Msa0342840 AT1G29380.4 58.750 80 33 0 160 239 147 226 4.16e-29 111
Msa0342840 AT1G29380.2 58.750 80 33 0 160 239 147 226 4.16e-29 111
Msa0342840 AT1G11820.2 46.032 126 64 2 148 269 368 493 2.25e-27 112
Msa0342840 AT4G05430.3 45.133 113 57 2 141 248 24 136 3.58e-27 104
Msa0342840 AT4G05430.1 49.462 93 46 1 157 248 19 111 6.14e-27 103
Msa0342840 AT3G58100.1 46.218 119 62 2 161 278 41 158 1.91e-26 103
Msa0342840 AT3G58100.2 46.218 119 62 2 161 278 117 234 5.65e-26 103
Msa0342840 AT4G05430.2 47.115 104 50 2 141 239 24 127 3.60e-25 98.2
Msa0342840 AT2G01630.1 44.340 106 58 1 154 258 353 458 5.68e-25 105
Msa0342840 AT4G26830.1 43.590 117 53 2 131 246 349 453 8.72e-25 103
Msa0342840 AT1G66250.1 37.415 147 81 4 154 297 361 499 9.68e-25 104
Msa0342840 AT4G26830.2 43.590 117 53 2 131 246 353 457 1.08e-24 103
Msa0342840 AT5G55180.1 52.222 90 42 1 158 246 369 458 1.86e-24 103
Msa0342840 AT2G05790.1 33.750 240 138 8 23 246 236 470 2.02e-24 103
Msa0342840 AT5G35740.1 47.368 95 46 3 154 245 23 116 1.02e-23 94.0
Msa0342840 AT4G13600.1 43.182 132 55 4 161 274 61 190 4.30e-23 95.5
Msa0342840 AT1G18650.1 46.237 93 48 2 157 248 17 108 2.82e-22 92.4
Msa0342840 AT1G11820.3 50.538 93 45 1 148 239 368 460 2.83e-22 97.1
Msa0342840 AT1G18650.2 46.237 93 48 2 157 248 17 108 3.36e-22 90.5
Msa0342840 AT5G55180.2 53.012 83 38 1 158 239 369 451 1.31e-21 95.1
Msa0342840 AT5G67460.1 38.571 140 70 5 113 244 246 377 2.57e-21 93.6
Msa0342840 AT4G34480.3 47.674 86 43 2 161 245 253 337 4.70e-21 92.8
Msa0342840 AT4G34480.1 47.674 86 43 2 161 245 364 448 6.65e-21 93.2
Msa0342840 AT2G01630.3 46.512 86 45 1 154 238 353 438 3.65e-20 90.9
Msa0342840 AT5G67460.2 38.519 135 67 5 113 239 246 372 7.16e-20 89.4
Msa0342840 AT5G24318.3 41.121 107 57 3 141 246 217 318 1.17e-19 88.2
Msa0342840 AT2G16230.1 44.118 102 52 3 161 261 366 463 1.31e-19 89.4
Msa0342840 AT5G24318.1 41.121 107 57 3 141 246 356 457 1.73e-19 89.0
Msa0342840 AT3G55430.1 42.553 94 52 2 152 244 357 449 1.81e-19 88.6
Msa0342840 AT5G63240.1 38.393 112 62 4 139 246 20 128 9.17e-19 81.3
Msa0342840 AT5G63250.1 40.816 98 53 3 153 246 34 130 1.22e-18 80.9
Msa0342840 AT1G66870.1 38.298 94 55 2 153 244 18 110 2.87e-18 79.3
Msa0342840 AT1G66870.2 38.298 94 55 2 153 244 45 137 3.67e-18 79.7
Msa0342840 AT2G04910.2 40.860 93 54 1 154 245 8 100 3.90e-18 78.6
Msa0342840 AT1G79480.2 44.706 85 45 2 161 244 310 393 5.94e-18 84.0
Msa0342840 AT1G79480.1 44.706 85 45 2 161 244 311 394 6.09e-18 84.0
Msa0342840 AT5G24318.2 42.424 99 51 3 141 238 356 449 1.08e-17 83.6
Msa0342840 AT1G66852.1 34.783 92 60 0 153 244 18 109 1.27e-17 77.4
Msa0342840 AT5G63225.1 41.860 86 48 2 160 244 24 108 1.53e-17 77.4
Msa0342840 AT2G43660.2 41.758 91 52 1 159 248 33 123 3.36e-17 76.6
Msa0342840 AT2G43660.1 41.758 91 52 1 159 248 32 122 3.59e-17 76.6
Msa0342840 AT4G16165.1 38.710 93 56 1 153 244 18 110 3.60e-17 76.3
Msa0342840 AT5G56590.1 31.250 144 83 3 161 303 369 497 4.53e-17 82.0
Msa0342840 AT2G39640.1 35.246 122 68 3 136 247 426 546 6.34e-17 81.6
Msa0342840 AT2G39640.1 40.659 91 52 2 153 242 351 440 1.74e-14 74.3
Msa0342840 AT2G43670.1 40.426 94 53 2 153 245 27 118 9.42e-17 75.5
Msa0342840 AT1G69295.1 45.349 86 44 3 157 241 17 100 9.62e-17 78.2
Msa0342840 AT4G09465.1 40.217 92 53 2 156 246 26 116 1.02e-16 75.1
Msa0342840 AT4G09464.1 40.217 92 53 2 156 246 26 116 1.14e-16 75.1
Msa0342840 AT4G09467.1 40.217 92 53 2 156 246 26 116 1.14e-16 75.1
Msa0342840 AT1G66855.1 39.535 86 51 1 160 244 25 110 1.44e-16 74.7
Msa0342840 AT4G09462.1 40.217 92 53 2 156 246 24 114 1.46e-16 74.7
Msa0342840 AT5G08000.2 48.387 93 46 2 154 245 33 124 1.70e-16 77.4
Msa0342840 AT3G23770.1 39.048 105 60 2 145 248 374 475 1.79e-16 80.1
Msa0342840 AT2G04910.1 41.379 87 50 1 154 239 8 94 2.56e-16 74.3
Msa0342840 AT1G69295.2 45.349 86 44 3 157 241 13 96 3.12e-16 76.6
Msa0342840 AT4G09466.1 40.217 92 53 2 156 246 26 116 3.24e-16 73.9
Msa0342840 AT4G09090.1 42.529 87 48 2 161 246 31 116 5.07e-16 73.6
Msa0342840 AT5G61130.1 43.333 90 49 2 157 245 17 105 5.38e-16 75.5
Msa0342840 AT3G13560.4 40.625 96 56 1 161 255 362 457 1.28e-15 77.4
Msa0342840 AT3G13560.1 40.625 96 56 1 161 255 362 457 1.28e-15 77.4
Msa0342840 AT3G13560.2 40.625 96 56 1 161 255 362 457 1.28e-15 77.4
Msa0342840 AT3G13560.5 40.625 96 56 1 161 255 362 457 1.28e-15 77.4
Msa0342840 AT3G13560.3 40.625 96 56 1 161 255 362 457 1.28e-15 77.4
Msa0342840 AT4G29360.2 38.202 89 54 1 160 247 400 488 1.51e-15 77.4
Msa0342840 AT5G53600.1 39.785 93 53 2 153 244 21 111 1.67e-15 71.6
Msa0342840 AT4G29360.1 38.202 89 54 1 160 247 390 478 1.78e-15 77.0
Msa0342840 AT5G08000.1 48.276 87 43 2 160 245 20 105 1.89e-15 73.9
Msa0342840 AT2G42930.1 39.241 79 47 1 161 239 40 117 9.60e-15 70.5
Msa0342840 AT1G78520.1 42.529 87 47 2 160 245 31 115 1.01e-14 69.7
Msa0342840 AT5G63230.1 37.209 86 52 2 160 244 25 109 1.05e-14 69.7
Msa0342840 AT4G14080.1 35.897 117 67 3 137 248 364 477 1.47e-14 74.3
Msa0342840 AT1G26450.1 45.455 77 39 3 157 232 17 91 1.70e-14 71.2
Msa0342840 AT2G03505.3 36.458 96 57 3 157 249 17 111 3.36e-14 68.6
Msa0342840 AT2G03505.4 36.170 94 56 3 157 247 17 109 4.29e-14 69.7
Msa0342840 AT2G03505.1 36.170 94 56 3 157 247 17 109 4.29e-14 69.7
Msa0342840 AT2G03505.2 36.170 94 56 3 157 247 17 109 4.29e-14 69.7
Msa0342840 AT5G53610.1 41.176 85 47 2 161 244 28 110 9.13e-14 67.0
Msa0342840 AT3G07320.1 39.785 93 55 1 157 248 367 459 1.17e-13 71.6
Msa0342840 AT1G13830.2 43.373 83 40 4 158 238 32 109 7.58e-13 67.0
Msa0342840 AT1G13830.1 43.750 80 38 4 161 238 21 95 1.69e-12 65.9
Msa0342840 AT3G04010.1 41.935 93 48 4 160 249 373 462 7.20e-12 66.2
Msa0342840 AT4G17180.1 38.372 86 49 3 160 244 358 440 1.05e-11 65.9
Msa0342840 AT5G58090.1 41.573 89 46 4 160 245 361 446 1.27e-11 65.5
Msa0342840 AT2G29735.1 35.870 92 55 3 156 244 26 116 7.94e-11 58.9

Find 130 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAAGAAATTGTGTTTC+TGG 0.151251 3_1:-866628 None:intergenic
ACTATGGTGTTGGTAAAATT+TGG 0.173757 3_1:+868993 Msa0342840:three_prime_UTR
ACATCATCTCCAGTAGGTTT+TGG 0.266537 3_1:+868446 Msa0342840:CDS
GCTGGATTTGTTGGTGATAA+TGG 0.267994 3_1:-867043 None:intergenic
TAGAAAGACAGTAGAGTAGT+AGG 0.271212 3_1:+868639 Msa0342840:three_prime_UTR
TTTGCTAAGCTGCATGGTTC+TGG 0.314068 3_1:+868517 Msa0342840:CDS
AGTCCTCCATCTTCTTCTTC+AGG 0.320635 3_1:+867199 Msa0342840:CDS
TTCAGGAACTGGAGTAGTTT+AGG 0.325211 3_1:+868765 Msa0342840:three_prime_UTR
TGCATCTTCCCATCAGCTTC+AGG 0.344512 3_1:+867571 Msa0342840:CDS
TTGTAACAGATCACAAGATT+TGG 0.357056 3_1:+866888 Msa0342840:CDS
TAGAGCATGGAAGCTAACTA+TGG 0.359132 3_1:-868575 None:intergenic
AGTGTAGATTGTGGTGTTCC+AGG 0.362624 3_1:-867244 None:intergenic
AATATGTTTCTTTGATACTA+AGG 0.363586 3_1:-866445 None:intergenic
TCTTGGCTATAATCAGATTC+AGG 0.369696 3_1:+868748 Msa0342840:three_prime_UTR
GCTTACAAATCCAACATCTT+AGG 0.371314 3_1:+869050 Msa0342840:three_prime_UTR
CCACCACCATCACCATTATC+AGG 0.376324 3_1:+867130 Msa0342840:CDS
GTTCCTGTTCCTCCTGATAA+TGG 0.379708 3_1:-867142 None:intergenic
ACCTTTATACCTTTAGTTCA+AGG 0.379755 3_1:+866536 Msa0342840:five_prime_UTR
GGTGAATTTGCTGGATTTGT+TGG 0.382630 3_1:-867052 None:intergenic
CATGGTTCTGGTGACATTAT+TGG 0.382811 3_1:+868529 Msa0342840:CDS
GCTGCAACAAGCTGTGACTT+TGG 0.388632 3_1:+867406 Msa0342840:CDS
TTGGATTCCTCTCATTCAAC+TGG 0.389358 3_1:+868485 Msa0342840:CDS
GGCACAGGCACAGGCACATC+CGG 0.389361 3_1:+867610 Msa0342840:CDS
TCCTTGAACTAAAGGTATAA+AGG 0.392920 3_1:-866537 None:intergenic
GGAACTGTTGGTGGTGTAAC+AGG 0.393025 3_1:-867079 None:intergenic
AGTGTGTGCATTGTTTGTTT+TGG 0.397338 3_1:+868802 Msa0342840:three_prime_UTR
TTGTAAAGAATATAGGATAT+TGG 0.399613 3_1:-869206 None:intergenic
AAGGCCTATCCTTGAACTAA+AGG 0.401117 3_1:-866545 None:intergenic
TGTGTTTCTGGAATTTAGAA+TGG 0.403661 3_1:-866616 None:intergenic
AGCAACAAATTTACTGTCAA+TGG 0.409812 3_1:-869235 None:intergenic
CATATGTAAATCAACAACTA+TGG 0.412229 3_1:+868977 Msa0342840:three_prime_UTR
GGAGGAGCTGGCGCAGGCAC+AGG 0.414477 3_1:+867595 Msa0342840:CDS
CTTCTTCTTCAGGAGGTAGC+TGG 0.414888 3_1:+867209 Msa0342840:CDS
GCTGGCGCAGGCACAGGCAC+AGG 0.415709 3_1:+867601 Msa0342840:CDS
TACAAAGAAAGAAACAATAA+AGG 0.417834 3_1:+866361 Msa0342840:five_prime_UTR
GACTATGCTTGTGGAACAAA+AGG 0.417962 3_1:+867280 Msa0342840:CDS
CTATAATCAGATTCAGGAAC+TGG 0.422203 3_1:+868754 Msa0342840:three_prime_UTR
TTCACTGGTGTTGTTCCTCC+TGG 0.427069 3_1:-867100 None:intergenic
TGACAGTGACAGCGTTGAAA+TGG 0.428428 3_1:-869160 None:intergenic
GAACTAAAGGTATAAAGGTT+AGG 0.428488 3_1:-866532 None:intergenic
GATGCAAGTAGAAGCTTCAT+TGG 0.437577 3_1:-866655 None:intergenic
GTTACACCACCAACAGTTCC+AGG 0.439114 3_1:+867082 Msa0342840:CDS
ACAGGTACTGGTGAATTTGC+TGG 0.439151 3_1:-867061 None:intergenic
AGAAATAACTGAGGACCTCT+TGG 0.442274 3_1:+868731 Msa0342840:three_prime_UTR
AAGTCACAGCTTGTTGCAGC+AGG 0.460540 3_1:-867403 None:intergenic
GTTGTTCCTCCTGGAACTGT+TGG 0.460619 3_1:-867091 None:intergenic
TCAGCTTCAGGAGGAGGAGC+TGG 0.463929 3_1:+867583 Msa0342840:CDS
GGTGTTGCACCTGGATTTGT+TGG 0.466876 3_1:-866986 None:intergenic
AAACACAATTTCTTTCATCA+TGG 0.468138 3_1:+866632 Msa0342840:exon
GGAATTTAGAATGGAGCTGT+TGG 0.469798 3_1:-866607 None:intergenic
ACAGCTGGTGGAGTAGTAGA+AGG 0.473175 3_1:-867019 None:intergenic
AAAATAAGTCATGTGGGTAC+TGG 0.473219 3_1:+868856 Msa0342840:three_prime_UTR
AGAAAGACAGTAGAGTAGTA+GGG 0.474208 3_1:+868640 Msa0342840:three_prime_UTR
AGAAGAAAATGAGAAAAGAT+AGG 0.477075 3_1:-866683 None:intergenic
CAGTGATGATTGTTGGTGAT+GGG 0.480237 3_1:-866931 None:intergenic
TCTGAATTTGCGAAAATGAA+AGG 0.480451 3_1:-869109 None:intergenic
AGCTGGTGTGTGGCTAAACC+TGG 0.480971 3_1:+867226 Msa0342840:CDS
GTTCCTCCTGGAACTGTTGG+TGG 0.482015 3_1:-867088 None:intergenic
TGGGCCGCAAAGCTCTCCAT+TGG 0.483468 3_1:+868466 Msa0342840:CDS
TCACCATTATCAGGAGGAAC+AGG 0.487525 3_1:+867139 Msa0342840:CDS
TTCCCATCAGCTTCAGGAGG+AGG 0.492744 3_1:+867577 Msa0342840:CDS
TCAGGAGGAGGAGCTGGCGC+AGG 0.502820 3_1:+867589 Msa0342840:CDS
CTTTGCGGCCCAAAACCTAC+TGG 0.504420 3_1:-868455 None:intergenic
CAACAAGCTGTGACTTTGGA+GGG 0.506706 3_1:+867410 Msa0342840:CDS
ATCTACACTTCAAACAGCAT+TGG 0.509235 3_1:+867258 Msa0342840:CDS
AGAAATAGTTGAAAATGCAT+TGG 0.517953 3_1:-866813 None:intergenic
AAGCTTGAGTAAACAGATCA+AGG 0.518696 3_1:-868687 None:intergenic
GATGGAGGACTTGTGCTTGA+AGG 0.519243 3_1:-867187 None:intergenic
GTAAAATCGTAGTGGTCTGA+TGG 0.520816 3_1:-869074 None:intergenic
GAGACCTTTGCTAAGCTGCA+TGG 0.522316 3_1:+868511 Msa0342840:CDS
TATACCTTTAGTTCAAGGAT+AGG 0.525984 3_1:+866541 Msa0342840:five_prime_UTR
GAAGGAACAGTGATGATTGT+TGG 0.527796 3_1:-866938 None:intergenic
GCAACAAGCTGTGACTTTGG+AGG 0.530607 3_1:+867409 Msa0342840:CDS
TCATTGTACTAACTAGTAGA+TGG 0.530763 3_1:+868890 Msa0342840:three_prime_UTR
GAAGGTGGAGGATTTGTGAC+AGG 0.530909 3_1:-867169 None:intergenic
TGTTATACAATTCTACAAGC+AGG 0.531967 3_1:+868614 Msa0342840:three_prime_UTR
TAAATCAACAACTATGGTGT+TGG 0.532644 3_1:+868983 Msa0342840:three_prime_UTR
GTTGGAGAAACTGTTGTGAC+AGG 0.533113 3_1:-866968 None:intergenic
GAATCCAATGGAGAGCTTTG+CGG 0.535900 3_1:-868470 None:intergenic
CCTGATAATGGTGATGGTGG+TGG 0.538366 3_1:-867130 None:intergenic
CTCCTCCTCCTGAAGCTGAT+GGG 0.539809 3_1:-867579 None:intergenic
TGAAGCTGATGGGAAGATGC+AGG 0.543799 3_1:-867569 None:intergenic
ATTCAAGAAAATAAGTCATG+TGG 0.550923 3_1:+868849 Msa0342840:three_prime_UTR
CTACCTCCTGAAGAAGAAGA+TGG 0.552545 3_1:-867205 None:intergenic
ACAGTGATGATTGTTGGTGA+TGG 0.553424 3_1:-866932 None:intergenic
GTTCCTCCTGATAATGGTGA+TGG 0.553903 3_1:-867136 None:intergenic
GCTCCTCCTCCTGAAGCTGA+TGG 0.558816 3_1:-867580 None:intergenic
GGACTTGTGCTTGAAGGTGG+AGG 0.559600 3_1:-867181 None:intergenic
GTTGGTGATAATGGAACAGC+TGG 0.559840 3_1:-867034 None:intergenic
GTAGTAGAAGGAACTGTGAC+AGG 0.561662 3_1:-867007 None:intergenic
CAGGCACAGGCACATCCGGC+GGG 0.564968 3_1:+867614 Msa0342840:CDS
AGAGTAGTAGGGAGAGTTTG+TGG 0.566599 3_1:+868651 Msa0342840:three_prime_UTR
ACAGTTTCTCCAACAAATCC+AGG 0.567466 3_1:+866977 Msa0342840:CDS
TTCAGGAGGTAGCTGGTGTG+TGG 0.575773 3_1:+867216 Msa0342840:CDS
TTCAAGAAAATAAGTCATGT+GGG 0.579136 3_1:+868850 Msa0342840:three_prime_UTR
TACAGCGAGGAGATAGAGCA+TGG 0.583522 3_1:-868588 None:intergenic
GTTGGTGGTGTAACAGGTAC+TGG 0.587227 3_1:-867073 None:intergenic
ACTGTGACAGGTGTTGCACC+TGG 0.594459 3_1:-866995 None:intergenic
GTTGTGACAGGATTGGTTGA+AGG 0.600451 3_1:-866956 None:intergenic
ACAGCATTGGACTATGCTTG+TGG 0.604056 3_1:+867271 Msa0342840:CDS
CCTCCATCTTCTTCTTCAGG+AGG 0.605110 3_1:+867202 Msa0342840:CDS
GTCTGATGGACCTAAGATGT+TGG 0.605429 3_1:-869060 None:intergenic
AACAAGCTGTGACTTTGGAG+GGG 0.609446 3_1:+867411 Msa0342840:CDS
AGAGCATGGAAGCTAACTAT+GGG 0.609588 3_1:-868574 None:intergenic
AGAAACTGTTGTGACAGGAT+TGG 0.611206 3_1:-866963 None:intergenic
CCTCCTGATAATGGTGATGG+TGG 0.614026 3_1:-867133 None:intergenic
TTATTGGTCAGTGGAAGACT+CGG 0.614232 3_1:+868545 Msa0342840:CDS
TGTTGAATGCAAAAGAAGCA+TGG 0.614920 3_1:-867363 None:intergenic
GATTGTAACAGCTACCACCT+TGG 0.617114 3_1:-867324 None:intergenic
GTTGAATGAGAGGAATCCAA+TGG 0.618258 3_1:-868482 None:intergenic
CTGGTGACATTATTGGTCAG+TGG 0.619927 3_1:+868536 Msa0342840:CDS
AGAACCATGCAGCTTAGCAA+AGG 0.620135 3_1:-868515 None:intergenic
TCTCAGACCAGTTGAATGAG+AGG 0.621991 3_1:-868492 None:intergenic
ACAGGCACAGGCACATCCGG+CGG 0.622496 3_1:+867613 Msa0342840:CDS
GGAGGACTTGTGCTTGAAGG+TGG 0.626099 3_1:-867184 None:intergenic
ATCTTCCCATCAGCTTCAGG+AGG 0.632762 3_1:+867574 Msa0342840:CDS
GGTGATAATGGAACAGCTGG+TGG 0.636040 3_1:-867031 None:intergenic
GGTGGTGGATAAGAATTCAC+TGG 0.638656 3_1:-867115 None:intergenic
GGTACTACATCATCTCCAGT+AGG 0.642872 3_1:+868440 Msa0342840:CDS
ACTAGGATTAGTGCTAGTAA+TGG 0.644779 3_1:-867440 None:intergenic
TGAATCTGATTATAGCCAAG+AGG 0.657778 3_1:-868746 None:intergenic
ATGTAAAAGTAAAATCGTAG+TGG 0.671959 3_1:-869082 None:intergenic
GATTGTTCACAAATAAACCA+AGG 0.679441 3_1:+867307 Msa0342840:CDS
CCTCCTGAAGAAGAAGATGG+AGG 0.682773 3_1:-867202 None:intergenic
CCACCATCACCATTATCAGG+AGG 0.700034 3_1:+867133 Msa0342840:CDS
TTCAACAGCAGAAATAACTG+AGG 0.709826 3_1:+868722 Msa0342840:three_prime_UTR
TGTTCACAAATAAACCAAGG+TGG 0.741309 3_1:+867310 Msa0342840:CDS
GCTGTTTGAAGTGTAGATTG+TGG 0.741949 3_1:-867253 None:intergenic
TTGTATAACAAACTACAGCG+AGG 0.756170 3_1:-868601 None:intergenic
ACACCACCAACAGTTCCAGG+AGG 0.766003 3_1:+867085 Msa0342840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTGTTTTAGAAATTTTA+AGG + chr3_1:867762-867781 Msa0342840:intron 15.0%
!!! TTTGATTTGATTTTATCTTT+TGG - chr3_1:867961-867980 None:intergenic 15.0%
!!! TTTTCAACTTATTTTCATAA+GGG - chr3_1:868218-868237 None:intergenic 15.0%
!! TACAAAGAAAGAAACAATAA+AGG + chr3_1:866361-866380 Msa0342840:five_prime_UTR 20.0%
!! AATATGTTTCTTTGATACTA+AGG - chr3_1:866448-866467 None:intergenic 20.0%
!! TGTAAAAATAGCATTAAACA+AGG - chr3_1:866758-866777 None:intergenic 20.0%
!! ACTATTATAGATATCTTACT+AGG - chr3_1:867460-867479 None:intergenic 20.0%
!! CAAGCATAATATGAAAAATT+AGG - chr3_1:867490-867509 None:intergenic 20.0%
!! ATTTGAATTGAGTATCTTAT+TGG - chr3_1:867701-867720 None:intergenic 20.0%
!! ATACTCAATTCAAATATCTA+CGG + chr3_1:867706-867725 Msa0342840:intron 20.0%
!!! TTACAAAAAAGACATTGATT+AGG - chr3_1:867788-867807 None:intergenic 20.0%
!! TAAGCTATAAGCTTAAAAAT+GGG - chr3_1:868041-868060 None:intergenic 20.0%
!! ATAAGCTATAAGCTTAAAAA+TGG - chr3_1:868042-868061 None:intergenic 20.0%
!!! ATATCTAAAACTAGCTTATA+GGG - chr3_1:868154-868173 None:intergenic 20.0%
!!! AATATCTAAAACTAGCTTAT+AGG - chr3_1:868155-868174 None:intergenic 20.0%
!!! GTTTTCAACTTATTTTCATA+AGG - chr3_1:868219-868238 None:intergenic 20.0%
!!! AATAAGTTGAAAACAACTTA+TGG + chr3_1:868225-868244 Msa0342840:intron 20.0%
!! TTGTAAAGAATATAGGATAT+TGG - chr3_1:869209-869228 None:intergenic 20.0%
!!! GCAATTTTTGTAAAGAATAT+AGG - chr3_1:869216-869235 None:intergenic 20.0%
!! ATTTTTCTTCTTTGTGCAAA+TGG + chr3_1:866391-866410 Msa0342840:five_prime_UTR 25.0%
!! TGATACTAAGGAAATGTTTT+TGG - chr3_1:866436-866455 None:intergenic 25.0%
!! TGTAGATGTAATGCTTTTAA+AGG - chr3_1:866567-866586 None:intergenic 25.0%
! AAACACAATTTCTTTCATCA+TGG + chr3_1:866632-866651 Msa0342840:exon 25.0%
! AGAAGAAAATGAGAAAAGAT+AGG - chr3_1:866686-866705 None:intergenic 25.0%
! AGAAATAGTTGAAAATGCAT+TGG - chr3_1:866816-866835 None:intergenic 25.0%
!! AATGCATTTTCAACTATTTC+TGG + chr3_1:866815-866834 Msa0342840:CDS 25.0%
!!! AGTATCTTATTGGCTTAAAA+AGG - chr3_1:867691-867710 None:intergenic 25.0%
!!! AAGTTGTTTTCACTTTTCAT+AGG + chr3_1:868000-868019 Msa0342840:intron 25.0%
!!! AGTTGTTTTCACTTTTCATA+GGG + chr3_1:868001-868020 Msa0342840:intron 25.0%
!! TATCTAAAACTAGCTTATAG+GGG - chr3_1:868153-868172 None:intergenic 25.0%
! ACTAAGCTTAATACATCAAT+AGG + chr3_1:868362-868381 Msa0342840:intron 25.0%
! ATTCAAGAAAATAAGTCATG+TGG + chr3_1:868849-868868 Msa0342840:three_prime_UTR 25.0%
! TTCAAGAAAATAAGTCATGT+GGG + chr3_1:868850-868869 Msa0342840:three_prime_UTR 25.0%
! CATATGTAAATCAACAACTA+TGG + chr3_1:868977-868996 Msa0342840:three_prime_UTR 25.0%
! ATGTAAAAGTAAAATCGTAG+TGG - chr3_1:869085-869104 None:intergenic 25.0%
! GAACTAAAGGTATAAAGGTT+AGG - chr3_1:866535-866554 None:intergenic 30.0%
! ACCTTTATACCTTTAGTTCA+AGG + chr3_1:866536-866555 Msa0342840:five_prime_UTR 30.0%
! TCCTTGAACTAAAGGTATAA+AGG - chr3_1:866540-866559 None:intergenic 30.0%
! TATACCTTTAGTTCAAGGAT+AGG + chr3_1:866541-866560 Msa0342840:five_prime_UTR 30.0%
!! TGTGTTTCTGGAATTTAGAA+TGG - chr3_1:866619-866638 None:intergenic 30.0%
GATGAAAGAAATTGTGTTTC+TGG - chr3_1:866631-866650 None:intergenic 30.0%
! TTTCTCATTTTCTTCTGTTC+TGG + chr3_1:866690-866709 Msa0342840:CDS 30.0%
!! GCTACTTTCAAATTCTTTTC+AGG + chr3_1:866788-866807 Msa0342840:intron 30.0%
TTGTAACAGATCACAAGATT+TGG + chr3_1:866888-866907 Msa0342840:CDS 30.0%
GATTGTTCACAAATAAACCA+AGG + chr3_1:867307-867326 Msa0342840:CDS 30.0%
!! GGAATGAGAACTTTGAAATA+TGG + chr3_1:867825-867844 Msa0342840:intron 30.0%
AGCATAATTTAGACCTTGTT+TGG + chr3_1:867862-867881 Msa0342840:intron 30.0%
TTAAGCTGTATATCCAAACA+AGG - chr3_1:867878-867897 None:intergenic 30.0%
TTGACTAAAAAAGACCAGTT+TGG + chr3_1:868077-868096 Msa0342840:intron 30.0%
!!! TTCTGAAACTGTTTTAGACA+TGG - chr3_1:868251-868270 None:intergenic 30.0%
GCTTAATACATCAATAGGTA+TGG + chr3_1:868367-868386 Msa0342840:intron 30.0%
TGTTATACAATTCTACAAGC+AGG + chr3_1:868614-868633 Msa0342840:three_prime_UTR 30.0%
TCATTGTACTAACTAGTAGA+TGG + chr3_1:868890-868909 Msa0342840:three_prime_UTR 30.0%
TAAATCAACAACTATGGTGT+TGG + chr3_1:868983-869002 Msa0342840:three_prime_UTR 30.0%
!! ACTATGGTGTTGGTAAAATT+TGG + chr3_1:868993-869012 Msa0342840:three_prime_UTR 30.0%
TCTGAATTTGCGAAAATGAA+AGG - chr3_1:869112-869131 None:intergenic 30.0%
!!! TTGAGATCTTGCTTTTTGGA+AGG - chr3_1:866850-866869 None:intergenic 35.0%
!!! GACTTTGAGATCTTGCTTTT+TGG - chr3_1:866854-866873 None:intergenic 35.0%
ATCTACACTTCAAACAGCAT+TGG + chr3_1:867258-867277 Msa0342840:CDS 35.0%
TGTTCACAAATAAACCAAGG+TGG + chr3_1:867310-867329 Msa0342840:CDS 35.0%
!!! GCATGGTTTTGAAGTGAATT+TGG - chr3_1:867349-867368 None:intergenic 35.0%
TGTTGAATGCAAAAGAAGCA+TGG - chr3_1:867366-867385 None:intergenic 35.0%
! ACTAGGATTAGTGCTAGTAA+TGG - chr3_1:867443-867462 None:intergenic 35.0%
! CTCTCTTTTGAATTGTGACT+AGG + chr3_1:867538-867557 Msa0342840:intron 35.0%
TTTGAATTGTGACTAGGTTC+TGG + chr3_1:867544-867563 Msa0342840:intron 35.0%
TAATGAAAGCTTTCCCTTCA+CGG - chr3_1:867650-867669 None:intergenic 35.0%
CAAAGTTCTCATTCCATCTA+AGG - chr3_1:867820-867839 None:intergenic 35.0%
TATAGGGGATGAACATATAG+TGG - chr3_1:868138-868157 None:intergenic 35.0%
ACATCAATAGGTATGGAACA+AGG + chr3_1:868374-868393 Msa0342840:intron 35.0%
CATCAATAGGTATGGAACAA+GGG + chr3_1:868375-868394 Msa0342840:intron 35.0%
TTGTATAACAAACTACAGCG+AGG - chr3_1:868604-868623 None:intergenic 35.0%
TAGAAAGACAGTAGAGTAGT+AGG + chr3_1:868639-868658 Msa0342840:three_prime_UTR 35.0%
AGAAAGACAGTAGAGTAGTA+GGG + chr3_1:868640-868659 Msa0342840:three_prime_UTR 35.0%
AAGCTTGAGTAAACAGATCA+AGG - chr3_1:868690-868709 None:intergenic 35.0%
TTCAACAGCAGAAATAACTG+AGG + chr3_1:868722-868741 Msa0342840:three_prime_UTR 35.0%
TGAATCTGATTATAGCCAAG+AGG - chr3_1:868749-868768 None:intergenic 35.0%
TCTTGGCTATAATCAGATTC+AGG + chr3_1:868748-868767 Msa0342840:three_prime_UTR 35.0%
CTATAATCAGATTCAGGAAC+TGG + chr3_1:868754-868773 Msa0342840:three_prime_UTR 35.0%
! AGTGTGTGCATTGTTTGTTT+TGG + chr3_1:868802-868821 Msa0342840:three_prime_UTR 35.0%
AAAATAAGTCATGTGGGTAC+TGG + chr3_1:868856-868875 Msa0342840:three_prime_UTR 35.0%
GCTTACAAATCCAACATCTT+AGG + chr3_1:869050-869069 Msa0342840:three_prime_UTR 35.0%
AAGGCCTATCCTTGAACTAA+AGG - chr3_1:866548-866567 None:intergenic 40.0%
! GGAATTTAGAATGGAGCTGT+TGG - chr3_1:866610-866629 None:intergenic 40.0%
GATGCAAGTAGAAGCTTCAT+TGG - chr3_1:866658-866677 None:intergenic 40.0%
!! CAGTGATGATTGTTGGTGAT+GGG - chr3_1:866934-866953 None:intergenic 40.0%
! ACAGTGATGATTGTTGGTGA+TGG - chr3_1:866935-866954 None:intergenic 40.0%
GAAGGAACAGTGATGATTGT+TGG - chr3_1:866941-866960 None:intergenic 40.0%
AGAAACTGTTGTGACAGGAT+TGG - chr3_1:866966-866985 None:intergenic 40.0%
ACAGTTTCTCCAACAAATCC+AGG + chr3_1:866977-866996 Msa0342840:CDS 40.0%
!! GCTGGATTTGTTGGTGATAA+TGG - chr3_1:867046-867065 None:intergenic 40.0%
!! GGTGAATTTGCTGGATTTGT+TGG - chr3_1:867055-867074 None:intergenic 40.0%
! GCTGTTTGAAGTGTAGATTG+TGG - chr3_1:867256-867275 None:intergenic 40.0%
GACTATGCTTGTGGAACAAA+AGG + chr3_1:867280-867299 Msa0342840:CDS 40.0%
GTAACCAAACCTACCTTAGA+TGG + chr3_1:867804-867823 Msa0342840:intron 40.0%
! CATTCCATCTAAGGTAGGTT+TGG - chr3_1:867811-867830 None:intergenic 40.0%
! GTTCTCATTCCATCTAAGGT+AGG - chr3_1:867816-867835 None:intergenic 40.0%
! CACTTTTCATAGGGTCTGTA+TGG + chr3_1:868010-868029 Msa0342840:intron 40.0%
CACATTAAGCGAGTCTGTTT+GGG - chr3_1:868310-868329 None:intergenic 40.0%
ACACATTAAGCGAGTCTGTT+TGG - chr3_1:868311-868330 None:intergenic 40.0%
!! GAACAAGGGAGTACTAATCA+TGG + chr3_1:868389-868408 Msa0342840:intron 40.0%
! ACATCATCTCCAGTAGGTTT+TGG + chr3_1:868446-868465 Msa0342840:CDS 40.0%
! CATCATCTCCAGTAGGTTTT+GGG + chr3_1:868447-868466 Msa0342840:CDS 40.0%
GTTGAATGAGAGGAATCCAA+TGG - chr3_1:868485-868504 None:intergenic 40.0%
TTGGATTCCTCTCATTCAAC+TGG + chr3_1:868485-868504 Msa0342840:CDS 40.0%
!! CATGGTTCTGGTGACATTAT+TGG + chr3_1:868529-868548 Msa0342840:CDS 40.0%
TTATTGGTCAGTGGAAGACT+CGG + chr3_1:868545-868564 Msa0342840:CDS 40.0%
AGAGCATGGAAGCTAACTAT+GGG - chr3_1:868577-868596 None:intergenic 40.0%
TAGAGCATGGAAGCTAACTA+TGG - chr3_1:868578-868597 None:intergenic 40.0%
AGAAATAACTGAGGACCTCT+TGG + chr3_1:868731-868750 Msa0342840:three_prime_UTR 40.0%
TTCAGGAACTGGAGTAGTTT+AGG + chr3_1:868765-868784 Msa0342840:three_prime_UTR 40.0%
GTAAAATCGTAGTGGTCTGA+TGG - chr3_1:869077-869096 None:intergenic 40.0%
!! GTTGTGACAGGATTGGTTGA+AGG - chr3_1:866959-866978 None:intergenic 45.0%
GTTGGAGAAACTGTTGTGAC+AGG - chr3_1:866971-866990 None:intergenic 45.0%
GTAGTAGAAGGAACTGTGAC+AGG - chr3_1:867010-867029 None:intergenic 45.0%
!! GTTGGTGATAATGGAACAGC+TGG - chr3_1:867037-867056 None:intergenic 45.0%
!! ACAGGTACTGGTGAATTTGC+TGG - chr3_1:867064-867083 None:intergenic 45.0%
!! GGTGGTGGATAAGAATTCAC+TGG - chr3_1:867118-867137 None:intergenic 45.0%
!! GTTCCTCCTGATAATGGTGA+TGG - chr3_1:867139-867158 None:intergenic 45.0%
TCACCATTATCAGGAGGAAC+AGG + chr3_1:867139-867158 Msa0342840:CDS 45.0%
GTTCCTGTTCCTCCTGATAA+TGG - chr3_1:867145-867164 None:intergenic 45.0%
AGTCCTCCATCTTCTTCTTC+AGG + chr3_1:867199-867218 Msa0342840:CDS 45.0%
CTACCTCCTGAAGAAGAAGA+TGG - chr3_1:867208-867227 None:intergenic 45.0%
AGTGTAGATTGTGGTGTTCC+AGG - chr3_1:867247-867266 None:intergenic 45.0%
! ACAGCATTGGACTATGCTTG+TGG + chr3_1:867271-867290 Msa0342840:CDS 45.0%
GATTGTAACAGCTACCACCT+TGG - chr3_1:867327-867346 None:intergenic 45.0%
CAACAAGCTGTGACTTTGGA+GGG + chr3_1:867410-867429 Msa0342840:CDS 45.0%
! AACAAGCTGTGACTTTGGAG+GGG + chr3_1:867411-867430 Msa0342840:CDS 45.0%
GAAGGTGACGTTTACCAAAC+TGG - chr3_1:868094-868113 None:intergenic 45.0%
! AGATAGCTTGTGGCTCTGTT+TGG - chr3_1:868190-868209 None:intergenic 45.0%
AACAGAGCCACAAGCTATCT+TGG + chr3_1:868190-868209 Msa0342840:intron 45.0%
! TAAGCGAGTCTGTTTGGGAT+TGG - chr3_1:868305-868324 None:intergenic 45.0%
! ATTCCCATCTTTTGCAGCTC+TGG + chr3_1:868419-868438 Msa0342840:intron 45.0%
GTACCAGAGCTGCAAAAGAT+GGG - chr3_1:868425-868444 None:intergenic 45.0%
AGTACCAGAGCTGCAAAAGA+TGG - chr3_1:868426-868445 None:intergenic 45.0%
GGTACTACATCATCTCCAGT+AGG + chr3_1:868440-868459 Msa0342840:CDS 45.0%
GAATCCAATGGAGAGCTTTG+CGG - chr3_1:868473-868492 None:intergenic 45.0%
TCTCAGACCAGTTGAATGAG+AGG - chr3_1:868495-868514 None:intergenic 45.0%
AGAACCATGCAGCTTAGCAA+AGG - chr3_1:868518-868537 None:intergenic 45.0%
TTTGCTAAGCTGCATGGTTC+TGG + chr3_1:868517-868536 Msa0342840:CDS 45.0%
CTGGTGACATTATTGGTCAG+TGG + chr3_1:868536-868555 Msa0342840:CDS 45.0%
AGAGTAGTAGGGAGAGTTTG+TGG + chr3_1:868651-868670 Msa0342840:three_prime_UTR 45.0%
! GTCTGATGGACCTAAGATGT+TGG - chr3_1:869063-869082 None:intergenic 45.0%
! TGACAGTGACAGCGTTGAAA+TGG - chr3_1:869163-869182 None:intergenic 45.0%
! GGTGTTGCACCTGGATTTGT+TGG - chr3_1:866989-867008 None:intergenic 50.0%
ACAGCTGGTGGAGTAGTAGA+AGG - chr3_1:867022-867041 None:intergenic 50.0%
!! GGTGATAATGGAACAGCTGG+TGG - chr3_1:867034-867053 None:intergenic 50.0%
!! GTTGGTGGTGTAACAGGTAC+TGG - chr3_1:867076-867095 None:intergenic 50.0%
!! GGAACTGTTGGTGGTGTAAC+AGG - chr3_1:867082-867101 None:intergenic 50.0%
GTTACACCACCAACAGTTCC+AGG + chr3_1:867082-867101 Msa0342840:CDS 50.0%
GTTGTTCCTCCTGGAACTGT+TGG - chr3_1:867094-867113 None:intergenic 50.0%
!! TTCACTGGTGTTGTTCCTCC+TGG - chr3_1:867103-867122 None:intergenic 50.0%
!! CCTGATAATGGTGATGGTGG+TGG - chr3_1:867133-867152 None:intergenic 50.0%
CCACCACCATCACCATTATC+AGG + chr3_1:867130-867149 Msa0342840:CDS 50.0%
!! CCTCCTGATAATGGTGATGG+TGG - chr3_1:867136-867155 None:intergenic 50.0%
CCACCATCACCATTATCAGG+AGG + chr3_1:867133-867152 Msa0342840:CDS 50.0%
GAAGGTGGAGGATTTGTGAC+AGG - chr3_1:867172-867191 None:intergenic 50.0%
GATGGAGGACTTGTGCTTGA+AGG - chr3_1:867190-867209 None:intergenic 50.0%
CCTCCTGAAGAAGAAGATGG+AGG - chr3_1:867205-867224 None:intergenic 50.0%
CCTCCATCTTCTTCTTCAGG+AGG + chr3_1:867202-867221 Msa0342840:CDS 50.0%
CTTCTTCTTCAGGAGGTAGC+TGG + chr3_1:867209-867228 Msa0342840:CDS 50.0%
!! AAGTCACAGCTTGTTGCAGC+AGG - chr3_1:867406-867425 None:intergenic 50.0%
GCTGCAACAAGCTGTGACTT+TGG + chr3_1:867406-867425 Msa0342840:CDS 50.0%
GCAACAAGCTGTGACTTTGG+AGG + chr3_1:867409-867428 Msa0342840:CDS 50.0%
TGAAGCTGATGGGAAGATGC+AGG - chr3_1:867572-867591 None:intergenic 50.0%
TGCATCTTCCCATCAGCTTC+AGG + chr3_1:867571-867590 Msa0342840:CDS 50.0%
ATCTTCCCATCAGCTTCAGG+AGG + chr3_1:867574-867593 Msa0342840:CDS 50.0%
GGGTAAGTGAGAACCGTGAA+GGG + chr3_1:867634-867653 Msa0342840:intron 50.0%
GCTGATAAGCTCGTGTGAGA+AGG - chr3_1:868112-868131 None:intergenic 50.0%
AAGGGCTCCAAGATAGCTTG+TGG - chr3_1:868200-868219 None:intergenic 50.0%
GAGACCTTTGCTAAGCTGCA+TGG + chr3_1:868511-868530 Msa0342840:CDS 50.0%
TACAGCGAGGAGATAGAGCA+TGG - chr3_1:868591-868610 None:intergenic 50.0%
! ACTGTGACAGGTGTTGCACC+TGG - chr3_1:866998-867017 None:intergenic 55.0%
ACACCACCAACAGTTCCAGG+AGG + chr3_1:867085-867104 Msa0342840:CDS 55.0%
GTTCCTCCTGGAACTGTTGG+TGG - chr3_1:867091-867110 None:intergenic 55.0%
GGACTTGTGCTTGAAGGTGG+AGG - chr3_1:867184-867203 None:intergenic 55.0%
GGAGGACTTGTGCTTGAAGG+TGG - chr3_1:867187-867206 None:intergenic 55.0%
TTCAGGAGGTAGCTGGTGTG+TGG + chr3_1:867216-867235 Msa0342840:CDS 55.0%
AGCTGGTGTGTGGCTAAACC+TGG + chr3_1:867226-867245 Msa0342840:CDS 55.0%
TTCCCATCAGCTTCAGGAGG+AGG + chr3_1:867577-867596 Msa0342840:CDS 55.0%
CTCCTCCTCCTGAAGCTGAT+GGG - chr3_1:867582-867601 None:intergenic 55.0%
CGGGTAAGTGAGAACCGTGA+AGG + chr3_1:867633-867652 Msa0342840:intron 55.0%
CTTTGCGGCCCAAAACCTAC+TGG - chr3_1:868458-868477 None:intergenic 55.0%
GCTCCTCCTCCTGAAGCTGA+TGG - chr3_1:867583-867602 None:intergenic 60.0%
TCAGCTTCAGGAGGAGGAGC+TGG + chr3_1:867583-867602 Msa0342840:CDS 60.0%
CACGGTTCTCACTTACCCGC+CGG - chr3_1:867632-867651 None:intergenic 60.0%
TGGGCCGCAAAGCTCTCCAT+TGG + chr3_1:868466-868485 Msa0342840:CDS 60.0%
GGCACAGGCACAGGCACATC+CGG + chr3_1:867610-867629 Msa0342840:CDS 65.0%
ACAGGCACAGGCACATCCGG+CGG + chr3_1:867613-867632 Msa0342840:CDS 65.0%
TCAGGAGGAGGAGCTGGCGC+AGG + chr3_1:867589-867608 Msa0342840:CDS 70.0%
CAGGCACAGGCACATCCGGC+GGG + chr3_1:867614-867633 Msa0342840:CDS 70.0%
GGAGGAGCTGGCGCAGGCAC+AGG + chr3_1:867595-867614 Msa0342840:CDS 75.0%
GCTGGCGCAGGCACAGGCAC+AGG + chr3_1:867601-867620 Msa0342840:CDS 75.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 866353 869242 866353 ID=Msa0342840;Name=Msa0342840
chr3_1 mRNA 866353 869242 866353 ID=Msa0342840-mRNA-1;Parent=Msa0342840;Name=Msa0342840-mRNA-1;_AED=0.05;_eAED=0.05;_QI=298|1|1|1|1|1|4|662|310
chr3_1 exon 866353 866711 866353 ID=Msa0342840-mRNA-1:exon:161;Parent=Msa0342840-mRNA-1
chr3_1 exon 866810 867460 866810 ID=Msa0342840-mRNA-1:exon:162;Parent=Msa0342840-mRNA-1
chr3_1 exon 867560 867635 867560 ID=Msa0342840-mRNA-1:exon:163;Parent=Msa0342840-mRNA-1
chr3_1 exon 868436 869242 868436 ID=Msa0342840-mRNA-1:exon:164;Parent=Msa0342840-mRNA-1
chr3_1 five_prime_UTR 866353 866650 866353 ID=Msa0342840-mRNA-1:five_prime_utr;Parent=Msa0342840-mRNA-1
chr3_1 CDS 866651 866711 866651 ID=Msa0342840-mRNA-1:cds;Parent=Msa0342840-mRNA-1
chr3_1 CDS 866810 867460 866810 ID=Msa0342840-mRNA-1:cds;Parent=Msa0342840-mRNA-1
chr3_1 CDS 867560 867635 867560 ID=Msa0342840-mRNA-1:cds;Parent=Msa0342840-mRNA-1
chr3_1 CDS 868436 868580 868436 ID=Msa0342840-mRNA-1:cds;Parent=Msa0342840-mRNA-1
chr3_1 three_prime_UTR 868581 869242 868581 ID=Msa0342840-mRNA-1:three_prime_utr;Parent=Msa0342840-mRNA-1
Gene Sequence

>Msa0342840

ATGGCCAATGAAGCTTCTACTTGCATCTTGTTCCTATCTTTTCTCATTTTCTTCTGTTCTGGAACCAATGCATTTTCAACTATTTCTGGAGCAAGATTCCTTCCAAAAAGCAAGATCTCAAAGTCTCAGATTCAGTTTCTTGTAACAGATCACAAGATTTGGAGAAAACTTCTAGACACAAACCCATCACCAACAATCATCACTGTTCCTTCAACCAATCCTGTCACAACAGTTTCTCCAACAAATCCAGGTGCAACACCTGTCACAGTTCCTTCTACTACTCCACCAGCTGTTCCATTATCACCAACAAATCCAGCAAATTCACCAGTACCTGTTACACCACCAACAGTTCCAGGAGGAACAACACCAGTGAATTCTTATCCACCACCATCACCATTATCAGGAGGAACAGGAACTGTTCCTGTCACAAATCCTCCACCTTCAAGCACAAGTCCTCCATCTTCTTCTTCAGGAGGTAGCTGGTGTGTGGCTAAACCTGGAACACCACAATCTACACTTCAAACAGCATTGGACTATGCTTGTGGAACAAAAGGAACTGATTGTTCACAAATAAACCAAGGTGGTAGCTGTTACAATCCAAATTCACTTCAAAACCATGCTTCTTTTGCATTCAACAGCTACTATGTAAAGAATCCTGCTGCAACAAGCTGTGACTTTGGAGGGGTTGCTACCATTACTAGCACTAATCCTAGTTCTGGTACCTGCATCTTCCCATCAGCTTCAGGAGGAGGAGCTGGCGCAGGCACAGGCACAGGCACATCCGGCGGCTCTGGTACTACATCATCTCCAGTAGGTTTTGGGCCGCAAAGCTCTCCATTGGATTCCTCTCATTCAACTGGTCTGAGACCTTTGCTAAGCTGCATGGTTCTGGTGACATTATTGGTCAGTGGAAGACTCGGCATGAGCCCATAG
Protein sequence

>Msa0342840

MANEASTCILFLSFLIFFCSGTNAFSTISGARFLPKSKISKSQIQFLVTDHKIWRKLLDTNPSPTIITVPSTNPVTTVSPTNPGATPVTVPSTTPPAVPLSPTNPANSPVPVTPPTVPGGTTPVNSYPPPSPLSGGTGTVPVTNPPPSSTSPPSSSSGGSWCVAKPGTPQSTLQTALDYACGTKGTDCSQINQGGSCYNPNSLQNHASFAFNSYYVKNPAATSCDFGGVATITSTNPSSGTCIFPSASGGGAGAGTGTGTSGGSGTTSSPVGFGPQSSPLDSSHSTGLRPLLSCMVLVTLLVSGRLGMSP*