AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0195440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195440 MtrunA17_Chr2g0286601 94.405 697 35 1 1 697 1 693 0.0 1362
Msa0195440 MtrunA17_Chr2g0286551 82.546 699 93 4 1 697 1 672 0.0 1144
Msa0195440 MtrunA17_Chr2g0286571 89.643 280 27 1 1 278 1 280 0.0 525
Msa0195440 MtrunA17_Chr3g0102001 36.694 744 400 20 5 693 18 745 1.52e-132 408
Msa0195440 MtrunA17_Chr5g0439061 34.672 747 397 18 9 693 23 740 1.46e-124 387
Msa0195440 MtrunA17_Chr2g0286561 87.317 205 26 0 493 697 14 218 4.82e-122 362
Msa0195440 MtrunA17_Chr3g0098481 45.425 306 159 4 395 693 37 341 9.94e-83 265
Msa0195440 MtrunA17_Chr2g0284501 27.059 680 430 10 13 676 18 647 8.14e-68 235
Msa0195440 MtrunA17_Chr3g0098531 42.606 284 154 5 417 693 12 293 4.01e-66 220
Msa0195440 MtrunA17_Chr3g0098301 39.203 301 175 4 395 688 103 402 4.69e-61 210
Msa0195440 MtrunA17_Chr5g0439051 53.000 200 93 1 494 693 20 218 2.10e-59 199
Msa0195440 MtrunA17_Chr5g0439081 47.423 194 100 1 496 689 18 209 1.04e-52 181
Msa0195440 MtrunA17_Chr2g0297701 33.663 303 195 3 381 679 265 565 3.35e-50 184
Msa0195440 MtrunA17_Chr2g0297701 28.641 206 137 3 9 214 48 243 2.46e-15 79.7
Msa0195440 MtrunA17_Chr5g0410271 37.201 293 173 4 375 657 15 306 4.78e-50 177
Msa0195440 MtrunA17_Chr5g0443001 34.948 289 187 1 394 682 313 600 1.93e-49 182
Msa0195440 MtrunA17_Chr5g0443001 24.107 224 156 3 5 218 21 240 6.24e-11 65.9
Msa0195440 MtrunA17_Chr5g0410201 35.390 308 194 4 375 679 16 321 3.62e-49 175
Msa0195440 MtrunA17_Chr5g0410171 36.149 296 171 4 394 679 440 727 3.12e-48 181
Msa0195440 MtrunA17_Chr5g0410171 25.221 226 140 8 16 219 133 351 3.04e-11 67.0
Msa0195440 MtrunA17_Chr6g0456141 33.333 315 196 5 373 682 75 380 7.25e-48 173
Msa0195440 MtrunA17_Chr5g0410461 34.494 316 190 4 373 679 151 458 2.24e-47 174
Msa0195440 MtrunA17_Chr2g0297491 33.016 315 193 6 382 692 78 378 2.33e-47 172
Msa0195440 MtrunA17_Chr5g0410341 36.177 293 176 4 375 657 434 725 4.89e-46 175
Msa0195440 MtrunA17_Chr5g0410341 26.457 223 136 8 5 207 119 333 3.31e-12 70.1
Msa0195440 MtrunA17_Chr2g0297481 33.779 299 190 5 382 679 176 467 8.94e-46 170
Msa0195440 MtrunA17_Chr2g0297691 35.417 288 183 3 394 679 401 687 4.44e-45 172
Msa0195440 MtrunA17_Chr2g0284541 35.093 322 201 5 372 693 331 644 9.66e-45 170
Msa0195440 MtrunA17_Chr2g0284541 25.214 234 148 6 13 228 8 232 2.67e-14 76.6
Msa0195440 MtrunA17_Chr2g0297651 33.439 314 193 6 382 692 391 691 1.75e-44 170
Msa0195440 MtrunA17_Chr5g0439071 26.930 531 328 13 12 495 26 543 2.74e-44 167
Msa0195440 MtrunA17_Chr5g0410371 36.207 290 173 5 394 675 1 286 4.27e-44 160
Msa0195440 MtrunA17_Chr2g0297681 34.983 303 182 6 394 692 399 690 9.25e-44 168
Msa0195440 MtrunA17_Chr6g0456101 32.390 318 201 5 373 685 432 740 2.37e-43 167
Msa0195440 MtrunA17_Chr3g0100301 38.776 196 120 0 482 677 208 403 3.79e-43 161
Msa0195440 MtrunA17_Chr6g0457101 29.936 314 209 4 377 685 380 687 2.32e-42 164
Msa0195440 MtrunA17_Chr2g0297711 30.796 289 190 4 397 679 238 522 2.92e-40 155
Msa0195440 MtrunA17_Chr2g0297711 35.833 120 71 2 9 127 51 165 1.64e-11 67.4
Msa0195440 MtrunA17_Chr5g0410191 38.587 184 112 1 496 679 4 186 8.49e-38 139
Msa0195440 MtrunA17_Chr5g0410581 34.520 281 172 5 394 665 499 776 1.20e-37 150
Msa0195440 MtrunA17_Chr3g0100741 29.181 281 198 1 394 674 308 587 4.28e-37 147
Msa0195440 MtrunA17_Chr3g0098541 30.556 360 199 12 35 367 2 337 1.81e-35 137
Msa0195440 MtrunA17_Chr3g0096941 28.114 281 201 1 394 674 309 588 9.89e-34 137
Msa0195440 MtrunA17_Chr3g0096941 25.359 209 142 3 2 200 20 224 1.47e-11 67.8
Msa0195440 MtrunA17_Chr5g0410541 38.816 152 92 1 496 647 25 175 1.28e-24 102
Msa0195440 MtrunA17_Chr5g0410511 33.333 183 112 3 493 671 5 181 2.76e-24 101
Msa0195440 MtrunA17_Chr5g0410451 31.915 188 124 2 496 679 43 230 1.73e-22 97.1
Msa0195440 MtrunA17_Chr5g0410181 37.398 123 70 1 553 668 2 124 4.58e-22 92.8
Msa0195440 MtrunA17_Chr3g0098291 33.673 196 122 5 14 204 32 224 4.65e-21 92.8
Msa0195440 MtrunA17_Chr5g0439021 36.306 157 96 2 12 164 26 182 9.30e-20 87.8
Msa0195440 MtrunA17_Chr5g0439041 31.579 152 97 2 351 495 18 169 1.44e-19 87.8
Msa0195440 MtrunA17_Chr3g0098511 38.776 98 58 1 598 693 1 98 9.51e-19 82.0
Msa0195440 MtrunA17_Chr1g0211531 25.338 296 210 7 397 684 243 535 5.29e-17 84.7
Msa0195440 MtrunA17_Chr1g0211531 27.027 185 126 5 13 195 27 204 5.00e-11 65.9
Msa0195440 MtrunA17_Chr3g0098521 32.903 155 98 3 14 165 1 152 9.98e-17 78.2
Msa0195440 MtrunA17_Chr3g0098491 32.468 154 98 3 14 164 1 151 8.18e-16 75.1
Msa0195440 MtrunA17_Chr1g0211541 23.197 319 232 7 382 697 207 515 9.91e-16 80.9
Msa0195440 MtrunA17_Chr2g0297611 37.778 90 55 1 500 589 1 89 9.07e-15 70.5
Msa0195440 MtrunA17_Chr5g0410211 27.000 200 125 5 28 212 3 196 5.50e-14 71.6
Msa0195440 MtrunA17_Chr5g0410291 26.923 182 115 6 42 212 17 191 2.76e-12 66.6
Msa0195440 MtrunA17_Chr2g0297521 35.366 82 52 1 489 570 4 84 1.40e-11 61.2
Msa0195440 MtrunA17_Chr1g0211511 24.038 312 224 7 384 686 7 314 2.67e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195440 AT3G18670.1 33.993 303 194 5 397 695 296 596 4.91e-48 179
Msa0195440 AT3G18670.1 27.313 227 155 4 9 230 53 274 1.78e-13 74.3
Msa0195440 AT3G18670.2 33.993 303 194 5 397 695 351 651 6.05e-48 179
Msa0195440 AT3G18670.2 28.910 211 143 3 9 214 53 261 1.63e-16 84.0
Msa0195440 AT5G35810.1 36.000 300 175 8 392 678 31 326 1.77e-45 166
Msa0195440 AT5G04730.1 34.768 302 184 5 381 675 285 580 3.21e-45 171
Msa0195440 AT5G04700.1 38.153 249 152 2 429 675 399 647 6.08e-43 165
Msa0195440 AT5G04690.1 34.572 269 171 4 431 696 359 625 4.71e-38 150
Msa0195440 AT3G54065.1 37.850 214 123 3 480 687 8 217 6.48e-38 141
Msa0195440 AT5G04690.3 34.572 269 171 4 431 696 379 645 6.87e-38 150
Msa0195440 AT5G04690.2 34.572 269 171 4 431 696 393 659 7.54e-38 150
Msa0195440 AT5G04680.2 35.271 258 155 4 429 675 263 519 4.09e-34 137
Msa0195440 AT5G04680.3 35.271 258 155 4 429 675 372 628 6.09e-34 138
Msa0195440 AT5G04680.4 35.271 258 155 4 429 675 386 642 6.88e-34 138
Msa0195440 AT5G04680.1 35.271 258 155 4 429 675 415 671 1.19e-33 137

Find 147 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTGGCACTTCAAATAAT+TGG 0.180342 2_1:-12286241 Msa0195440:CDS
AACCATTGCTCGCGAAATTT+TGG 0.216103 2_1:-12285477 Msa0195440:intron
ATCTTTGCCTCCAATACTTC+AGG 0.230897 2_1:+12286831 None:intergenic
CAGACATGTTGCAGCATATA+TGG 0.231231 2_1:+12283038 None:intergenic
GAACAAGAATGTTGTTCTTT+TGG 0.248304 2_1:-12284213 Msa0195440:CDS
AGTCCTCATGTTGAAGAATT+TGG 0.251360 2_1:-12284410 Msa0195440:CDS
TTTCTATGAACGTTATAATA+AGG 0.259052 2_1:-12283496 Msa0195440:CDS
CCTTCATATCTTTGTTGAAT+AGG 0.261530 2_1:-12285673 Msa0195440:CDS
AACCCTACAAGAAGCTTTCT+TGG 0.265236 2_1:+12283156 None:intergenic
TTATAACGTTCATAGAAATT+AGG 0.268291 2_1:+12283501 None:intergenic
TGCATCTTGCTGCTACTTAT+AGG 0.285788 2_1:-12284088 Msa0195440:CDS
TGAGCAAGATTCTGCAGTTT+TGG 0.300345 2_1:+12283401 None:intergenic
GCAACTTTATGAGGCCCTTT+TGG 0.312018 2_1:-12282766 Msa0195440:three_prime_UTR
GTGCAGCTTCCAAAACAATT+AGG 0.314136 2_1:+12286148 None:intergenic
GCTAGGAAGCCTAATTGTTT+TGG 0.323574 2_1:-12286157 Msa0195440:CDS
ACAATTTCATCTTATCTTGT+TGG 0.333720 2_1:-12282678 Msa0195440:three_prime_UTR
TTGTAAAAGAAGGTAGTAAA+TGG 0.341101 2_1:-12283428 Msa0195440:CDS
TCTGTACTTTGATCTGAGTT+TGG 0.341942 2_1:-12282983 Msa0195440:CDS
ACCAAAGTATTCACTGGATA+TGG 0.355789 2_1:+12286446 None:intergenic
AATGAGGATGGGATCTCTTC+TGG 0.357932 2_1:+12286620 None:intergenic
CATCACTATCCATTGAACTA+TGG 0.365853 2_1:-12285707 Msa0195440:CDS
TGGTTAGCTAGCTCTTAAAA+TGG 0.369012 2_1:-12286934 Msa0195440:exon
TTACCAAGAAAGCTTCTTGT+AGG 0.369536 2_1:-12283159 Msa0195440:CDS
GATAAACTGCAAATCTCATC+TGG 0.369859 2_1:+12283061 None:intergenic
GGATGTTCCTGCACATATCT+TGG 0.372166 2_1:+12286724 None:intergenic
TATACAGTTTGTTAAATAAG+AGG 0.373856 2_1:-12284166 Msa0195440:CDS
TTTACAGCATTCACAAGCTT+TGG 0.376948 2_1:+12286211 None:intergenic
TTCAAACCGAGATGTGAGTA+TGG 0.377547 2_1:+12283203 None:intergenic
ATCACTGGTTCCCACCCAAT+TGG 0.380459 2_1:-12284917 Msa0195440:CDS
TGTGAAATCATACTTGTATA+AGG 0.382875 2_1:+12284439 None:intergenic
GAAGAATTTGGTGGTGGTGG+TGG 0.390543 2_1:-12284398 Msa0195440:CDS
GTCTGCAAGTGTTCATTGTT+AGG 0.397301 2_1:+12282834 None:intergenic
TCATTGTTAGGAGATATCTA+AGG 0.398711 2_1:+12282846 None:intergenic
CACATCTATAGCATCTGTTC+TGG 0.402327 2_1:-12283121 Msa0195440:CDS
TCGGATCATGGATTCCCAAT+TGG 0.407165 2_1:+12284902 None:intergenic
GTTCTAACCATTGGAAAGCT+TGG 0.411070 2_1:-12286954 Msa0195440:five_prime_UTR
GTATGGAAAGAAACAAAATA+AGG 0.413629 2_1:+12283220 None:intergenic
CGGATCATGGATTCCCAATT+GGG 0.415680 2_1:+12284903 None:intergenic
CTTCATGTTGCAGCAGAGCT+TGG 0.417345 2_1:-12286669 Msa0195440:CDS
AGGCAATATGCAACTTTATG+AGG 0.418327 2_1:-12282775 Msa0195440:three_prime_UTR
TATCTTAATTGCAGCAAAGA+TGG 0.421596 2_1:-12284303 Msa0195440:CDS
ATCTTTGCTGCAATTAAGAT+AGG 0.424483 2_1:+12284305 None:intergenic
TTCATGTTGCAGCAGAGCTT+GGG 0.430517 2_1:-12286668 Msa0195440:CDS
TAGTCTGAGTTTGTGCTTGA+AGG 0.435141 2_1:+12285732 None:intergenic
GATTCTTGATCAGGACCGCC+TGG 0.440459 2_1:+12283327 None:intergenic
CTTATAGGAGGTTTAAGCCT+TGG 0.448660 2_1:-12284073 Msa0195440:CDS
TTTCGTCAAGTTGATATCAA+TGG 0.450346 2_1:-12284122 Msa0195440:CDS
TCTTTGCCTCCAATACTTCA+GGG 0.455629 2_1:+12286832 None:intergenic
TTTAAGCCTTGGCGTGTGCC+TGG 0.460405 2_1:-12284062 Msa0195440:CDS
TCACTGGTTCCCACCCAATT+GGG 0.470490 2_1:-12284916 Msa0195440:CDS
TATGAACGTTATAATAAGGA+TGG 0.471736 2_1:-12283492 Msa0195440:CDS
CGAAATTTCGATAGGAAATG+AGG 0.474414 2_1:+12286604 None:intergenic
AAAATGAAGTTATTGAGAAG+AGG 0.477595 2_1:-12284340 Msa0195440:CDS
TGCATTTGCATCGCAGCACC+AGG 0.478373 2_1:+12284044 None:intergenic
GTTGAAGAATTTGGTGGTGG+TGG 0.479544 2_1:-12284401 Msa0195440:CDS
TACCGACACCGAAAATGATT+AGG 0.481204 2_1:+12284853 None:intergenic
ACCATATCCAGTGAATACTT+TGG 0.482222 2_1:-12286447 Msa0195440:intron
GGTATGCCGGATTCTTGATC+AGG 0.485024 2_1:+12283318 None:intergenic
TATGGAAAGAAACAAAATAA+GGG 0.487722 2_1:+12283221 None:intergenic
TTCTTTCCATACTCACATCT+CGG 0.490447 2_1:-12283209 Msa0195440:CDS
TTCTCTCCATTGCCCTCTCA+TGG 0.496208 2_1:+12286878 None:intergenic
CCTATTCAACAAAGATATGA+AGG 0.498588 2_1:+12285673 None:intergenic
GGCGGTCCTGATCAAGAATC+CGG 0.500801 2_1:-12283324 Msa0195440:CDS
ACACACAATGTTCTAACCAT+TGG 0.504628 2_1:-12286963 Msa0195440:five_prime_UTR
GTTCTGGTCTCATTCTGTGC+AGG 0.507575 2_1:-12283105 Msa0195440:CDS
CTAAGAATGCAAAACTCAAA+GGG 0.508121 2_1:-12286702 Msa0195440:CDS
TCCATTGCCCTCTCATGGCA+TGG 0.508412 2_1:+12286883 None:intergenic
TACCAAAATTTCGCGAGCAA+TGG 0.510348 2_1:+12285475 None:intergenic
CAAGTGGTGCGTGTGTATCA+AGG 0.510861 2_1:+12283448 None:intergenic
TTCATAGAAATTAGGTGGCA+TGG 0.511469 2_1:+12283509 None:intergenic
AGACATGTTGCAGCATATAT+GGG 0.513588 2_1:+12283039 None:intergenic
ACTTCTAATCCTTGCAATGA+AGG 0.515634 2_1:-12284880 Msa0195440:CDS
CCTAAGAATGCAAAACTCAA+AGG 0.517719 2_1:-12286703 Msa0195440:CDS
AGTCATCATCTTTGTTGTGT+TGG 0.519193 2_1:+12286511 None:intergenic
ATCTTAATTGCAGCAAAGAT+GGG 0.523320 2_1:-12284302 Msa0195440:CDS
CAAAATAAGGGCTGTGACGG+AGG 0.524058 2_1:+12283233 None:intergenic
ATTTCGATAGGAAATGAGGA+TGG 0.526430 2_1:+12286608 None:intergenic
AAGGATTAGAAGTCGGATCA+TGG 0.527536 2_1:+12284890 None:intergenic
ATGCATGTTCCATAGTTCAA+TGG 0.529696 2_1:+12285698 None:intergenic
ACATAAGCCAAGATATGTGC+AGG 0.531025 2_1:-12286731 Msa0195440:CDS
TACCAAGAAAGCTTCTTGTA+GGG 0.531662 2_1:-12283158 Msa0195440:CDS
CATTGTTAGGAGATATCTAA+GGG 0.536400 2_1:+12282847 None:intergenic
TTATACAAGTATGATTTCAC+AGG 0.538164 2_1:-12284437 Msa0195440:CDS
ACACTAACCAAAGTATTCAC+TGG 0.538217 2_1:+12286440 None:intergenic
AGTGCACAATGAGAACCAAA+AGG 0.538252 2_1:+12282751 None:intergenic
CACCTCTGCATATTCTTGCT+AGG 0.541079 2_1:-12286174 Msa0195440:CDS
TGAACAGCTGCCAAGAAAAG+AGG 0.541803 2_1:+12286570 None:intergenic
AGCTCTGCTGCAACATGAAG+AGG 0.542273 2_1:+12286672 None:intergenic
ATCTTGCTGCTACTTATAGG+AGG 0.551809 2_1:-12284085 Msa0195440:CDS
CACGCACCACTTGTAAAAGA+AGG 0.553960 2_1:-12283438 Msa0195440:CDS
AGTTATTGAGAAGAGGAGAA+TGG 0.554778 2_1:-12284333 Msa0195440:CDS
TTAGGAAGACCTTCATTGCA+AGG 0.555765 2_1:+12284871 None:intergenic
CATGTTGAAGAATTTGGTGG+TGG 0.560650 2_1:-12284404 Msa0195440:CDS
TCCACATCATGAATAGCCAC+AGG 0.562021 2_1:+12284242 None:intergenic
TTGGCTTATGTTATCTAACA+AGG 0.563339 2_1:+12286743 None:intergenic
GATCAAAGTACAGAGGGAGT+TGG 0.566389 2_1:+12282992 None:intergenic
AATTCTTCAACATGAGGACT+TGG 0.567474 2_1:+12284413 None:intergenic
TTGTTTAACCATGCCATGAG+AGG 0.567650 2_1:-12286891 Msa0195440:CDS
AAACAAAATAAGGGCTGTGA+CGG 0.571099 2_1:+12283230 None:intergenic
GTGCACAATGAGAACCAAAA+GGG 0.574034 2_1:+12282752 None:intergenic
GCTCTGCTGCAACATGAAGA+GGG 0.574081 2_1:+12286673 None:intergenic
GCAGCACCAGGCACACGCCA+AGG 0.574147 2_1:+12284056 None:intergenic
ATGTAGAGCAAGCGCAAGCA+AGG 0.575164 2_1:-12284953 Msa0195440:intron
TGTTTAACCATGCCATGAGA+GGG 0.575955 2_1:-12286890 Msa0195440:CDS
ATTTCCTATCGAAATTTCGA+AGG 0.577067 2_1:-12286600 Msa0195440:CDS
GAGTTTGGCTATGTGTCGGA+AGG 0.578667 2_1:-12282968 Msa0195440:CDS
ATGAACGTTATAATAAGGAT+GGG 0.580533 2_1:-12283491 Msa0195440:CDS
ACCGCCTGGGATAGCTGTTG+AGG 0.581995 2_1:+12283341 None:intergenic
ATCATGGATTCCCAATTGGG+TGG 0.589546 2_1:+12284906 None:intergenic
TTCGCCTTCGAAATTTCGAT+AGG 0.591191 2_1:+12286596 None:intergenic
TTCAATTGTTGATGAGAACA+AGG 0.592418 2_1:-12285768 Msa0195440:CDS
ATCTGAGTTTGGCTATGTGT+CGG 0.593043 2_1:-12282972 Msa0195440:CDS
TTTCGATAGGAAATGAGGAT+GGG 0.593948 2_1:+12286609 None:intergenic
ATTCTTGATCAGGACCGCCT+GGG 0.594512 2_1:+12283328 None:intergenic
AACTCAGATCAAAGTACAGA+GGG 0.594945 2_1:+12282986 None:intergenic
TAACGTTCATAGAAATTAGG+TGG 0.595282 2_1:+12283504 None:intergenic
TAGCTAACCAAGCTTTCCAA+TGG 0.596585 2_1:+12286947 None:intergenic
CCCTGTGGCTATTCATGATG+TGG 0.598098 2_1:-12284243 Msa0195440:CDS
TTTAACAAACTGTATACATG+AGG 0.601178 2_1:+12284173 None:intergenic
GTGGGAATACAAGTGGTACA+AGG 0.606330 2_1:-12284021 Msa0195440:intron
TCATGGATTCCCAATTGGGT+GGG 0.611555 2_1:+12284907 None:intergenic
TGCTGCGATGCAAATGCAGT+GGG 0.612632 2_1:-12284039 Msa0195440:CDS
ATAAACTGCAAATCTCATCT+GGG 0.613516 2_1:+12283062 None:intergenic
CCTCATGTTGAAGAATTTGG+TGG 0.614175 2_1:-12284407 Msa0195440:CDS
TCATTGCAAGGATTAGAAGT+CGG 0.619332 2_1:+12284883 None:intergenic
ACAGCATAGAGTTTGAAAGC+AGG 0.620300 2_1:+12283279 None:intergenic
AGAGAAAATCCAAAGGAGGA+AGG 0.622162 2_1:-12284513 Msa0195440:CDS
GCAGAGGTGATAATCCATCA+TGG 0.623629 2_1:+12286188 None:intergenic
CATTGACATAGGAGCAACAT+GGG 0.626539 2_1:-12284537 Msa0195440:intron
ACAACCTCAACAGCTATCCC+AGG 0.633602 2_1:-12283345 Msa0195440:CDS
GTGAATGCTGTAAACCATGA+TGG 0.647679 2_1:-12286202 Msa0195440:CDS
CCACCAAATTCTTCAACATG+AGG 0.652444 2_1:+12284407 None:intergenic
GGGTAGAGAAAATCCAAAGG+AGG 0.656067 2_1:-12284517 Msa0195440:CDS
CTCTGCTGCAACATGAAGAG+GGG 0.656516 2_1:+12286674 None:intergenic
AAATGCAGTGGGAATACAAG+TGG 0.670083 2_1:-12284028 Msa0195440:CDS
ACCATGCCATGAGAGGGCAA+TGG 0.670926 2_1:-12286884 Msa0195440:CDS
AACACTTTGTAGCTGCGCTG+AGG 0.674861 2_1:+12282943 None:intergenic
GTGCTGCGATGCAAATGCAG+TGG 0.675008 2_1:-12284040 Msa0195440:CDS
AAACTCAGATCAAAGTACAG+AGG 0.675417 2_1:+12282985 None:intergenic
GCATTGACATAGGAGCAACA+TGG 0.681991 2_1:-12284538 Msa0195440:intron
AACTGCAGAATCTTGCTCAG+TGG 0.683105 2_1:-12283397 Msa0195440:CDS
GTACAGAGGGAGTTGGACCA+AGG 0.683296 2_1:+12282999 None:intergenic
TTCCTAGCAAGAATATGCAG+AGG 0.691547 2_1:+12286172 None:intergenic
CATGGGTAGAGAAAATCCAA+AGG 0.708498 2_1:-12284520 Msa0195440:CDS
CCACATCATGAATAGCCACA+GGG 0.710076 2_1:+12284243 None:intergenic
GATTCTAGATATGTACCCTG+TGG 0.719569 2_1:-12284258 Msa0195440:CDS
ACCTCAACAGCTATCCCAGG+CGG 0.731295 2_1:-12283342 Msa0195440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTATTTAATTTTTACT+CGG - chr2_1:12284016-12284035 Msa0195440:intron 10.0%
!!! TTTATTTTTCATTTAGTATA+TGG - chr2_1:12284580-12284599 Msa0195440:intron 10.0%
!! TAATTGATTAAATTATGTAT+GGG - chr2_1:12286049-12286068 Msa0195440:intron 10.0%
!!! GCAAATTATTTTATGAAATA+TGG - chr2_1:12283304-12283323 Msa0195440:CDS 15.0%
!!! TATATTTTTTCTTTTCAACA+AGG - chr2_1:12283580-12283599 Msa0195440:intron 15.0%
!! AATAATCATATAAACAATGT+AGG + chr2_1:12283610-12283629 None:intergenic 15.0%
!! ATTATAAAATAAAATCAGGT+TGG + chr2_1:12283642-12283661 None:intergenic 15.0%
!! TCACATTATAAAATAAAATC+AGG + chr2_1:12283646-12283665 None:intergenic 15.0%
!!! TGATTTTATTTTATAATGTG+AGG - chr2_1:12283645-12283664 Msa0195440:intron 15.0%
!!! TTTTAGATATGCTTTTATTA+TGG + chr2_1:12283719-12283738 None:intergenic 15.0%
!! TGTAAAAAAATTTATATCGA+CGG - chr2_1:12283773-12283792 Msa0195440:intron 15.0%
!! TGAATTTACAATATTTGAAA+TGG + chr2_1:12284328-12284347 None:intergenic 15.0%
!! TTTCAAATATTGTAAATTCA+TGG - chr2_1:12284328-12284347 Msa0195440:CDS 15.0%
!! AAATTACAGATAAATATGAT+TGG - chr2_1:12284413-12284432 Msa0195440:CDS 15.0%
!!! TGAAGAAATATTAAGTTTTA+GGG + chr2_1:12284939-12284958 None:intergenic 15.0%
!!! AATTTTTTTGTTTAACTGAA+TGG - chr2_1:12285078-12285097 Msa0195440:intron 15.0%
!! CATACATAATTTAATCAATT+AGG + chr2_1:12286050-12286069 None:intergenic 15.0%
!! CTAATTGATTAAATTATGTA+TGG - chr2_1:12286048-12286067 Msa0195440:intron 15.0%
!!! TAAGAAATTTTTTAAGTGTT+AGG - chr2_1:12286843-12286862 Msa0195440:CDS 15.0%
!! AGTGTAATGAATTTATATGT+AGG - chr2_1:12283376-12283395 Msa0195440:CDS 20.0%
!! AATGAATTTATATGTAGGTT+TGG - chr2_1:12283381-12283400 Msa0195440:CDS 20.0%
!! AATTCAAAATTAATCTCTTC+CGG - chr2_1:12283805-12283824 Msa0195440:intron 20.0%
!! TAAAAGATGAAATTCAATTG+CGG + chr2_1:12284528-12284547 None:intergenic 20.0%
!!! GAAGAAATATTAAGTTTTAG+GGG + chr2_1:12284938-12284957 None:intergenic 20.0%
!!! GTGAAGAAATATTAAGTTTT+AGG + chr2_1:12284940-12284959 None:intergenic 20.0%
!! TATAGAATATAGACCATATA+AGG + chr2_1:12284975-12284994 None:intergenic 20.0%
!! CAGTTAAACAAAAAAATTAC+AGG + chr2_1:12285076-12285095 None:intergenic 20.0%
!!! TATACAGTTTGTTAAATAAG+AGG - chr2_1:12285472-12285491 Msa0195440:intron 20.0%
!!! ATTTTTGTTGATCATGTTAT+TGG - chr2_1:12285693-12285712 Msa0195440:CDS 20.0%
!! TTATAACGTTCATAGAAATT+AGG + chr2_1:12286140-12286159 None:intergenic 20.0%
!! TTTCTATGAACGTTATAATA+AGG - chr2_1:12286142-12286161 Msa0195440:CDS 20.0%
!! TATGGAAAGAAACAAAATAA+GGG + chr2_1:12286420-12286439 None:intergenic 20.0%
!! TTGTAACATCACTTAATATA+TGG + chr2_1:12286928-12286947 None:intergenic 20.0%
! TCACTTTCAATCAAAAACAA+TGG - chr2_1:12283149-12283168 Msa0195440:CDS 25.0%
!! TCGTGTAATTTTTATGTGAT+CGG + chr2_1:12283234-12283253 None:intergenic 25.0%
! TGATACTGATAAGTGATAAA+AGG - chr2_1:12283828-12283847 Msa0195440:intron 25.0%
!! ATATACTGGCTATAACTATA+AGG + chr2_1:12284056-12284075 None:intergenic 25.0%
! AGAAAAATAGTAGACAAGTT+AGG + chr2_1:12284085-12284104 None:intergenic 25.0%
!! ATGATTTTTAATCGTAGTTG+TGG - chr2_1:12284375-12284394 Msa0195440:CDS 25.0%
! AGACCTCAAAAACATTAATA+TGG - chr2_1:12284476-12284495 Msa0195440:CDS 25.0%
!!! ACACCATATTAATGTTTTTG+AGG + chr2_1:12284482-12284501 None:intergenic 25.0%
! ACAGATCAGTAAAAATACAA+AGG - chr2_1:12284547-12284566 Msa0195440:intron 25.0%
! CAAAAACTGTTATATTTGCA+AGG + chr2_1:12284874-12284893 None:intergenic 25.0%
!! CTTGCAAATATAACAGTTTT+TGG - chr2_1:12284872-12284891 Msa0195440:CDS 25.0%
!!! CTTTAGAATTAGTCCTTATA+TGG - chr2_1:12284959-12284978 Msa0195440:intron 25.0%
! AATCCATCCATATAAGAATT+AGG - chr2_1:12285045-12285064 Msa0195440:intron 25.0%
! TTTCCTAATTCTTATATGGA+TGG + chr2_1:12285051-12285070 None:intergenic 25.0%
! TGTGAAATCATACTTGTATA+AGG + chr2_1:12285202-12285221 None:intergenic 25.0%
! TTATACAAGTATGATTTCAC+AGG - chr2_1:12285201-12285220 Msa0195440:intron 25.0%
! AAAATGAAGTTATTGAGAAG+AGG - chr2_1:12285298-12285317 Msa0195440:intron 25.0%
! TTTAACAAACTGTATACATG+AGG + chr2_1:12285468-12285487 None:intergenic 25.0%
! AAGATTTCTAATCTATTAGG+AGG + chr2_1:12285657-12285676 None:intergenic 25.0%
! GTGAAGATTTCTAATCTATT+AGG + chr2_1:12285660-12285679 None:intergenic 25.0%
! TGGTTAATTAAATTGCTGAT+TGG - chr2_1:12285934-12285953 Msa0195440:intron 25.0%
! TAGTTACTCATAAAGTAAAG+TGG - chr2_1:12285972-12285991 Msa0195440:intron 25.0%
!!! TTGTATTTTTCAGCTTGTTA+AGG - chr2_1:12286103-12286122 Msa0195440:intron 25.0%
! TATGAACGTTATAATAAGGA+TGG - chr2_1:12286146-12286165 Msa0195440:CDS 25.0%
! ATGAACGTTATAATAAGGAT+GGG - chr2_1:12286147-12286166 Msa0195440:CDS 25.0%
! TTGTAAAAGAAGGTAGTAAA+TGG - chr2_1:12286210-12286229 Msa0195440:CDS 25.0%
! GTATGGAAAGAAACAAAATA+AGG + chr2_1:12286421-12286440 None:intergenic 25.0%
! ACAATTTCATCTTATCTTGT+TGG - chr2_1:12286960-12286979 Msa0195440:five_prime_UTR 25.0%
TTGGCTTATGTTATCTAACA+AGG + chr2_1:12282898-12282917 None:intergenic 30.0%
CTAAGAATGCAAAACTCAAA+GGG - chr2_1:12282936-12282955 Msa0195440:CDS 30.0%
ATTTCCTATCGAAATTTCGA+AGG - chr2_1:12283038-12283057 Msa0195440:CDS 30.0%
TTATCACTTATCAGTATCAC+CGG + chr2_1:12283827-12283846 None:intergenic 30.0%
! TTCAATTGTTGATGAGAACA+AGG - chr2_1:12283870-12283889 Msa0195440:intron 30.0%
CCTATTCAACAAAGATATGA+AGG + chr2_1:12283968-12283987 None:intergenic 30.0%
CCTTCATATCTTTGTTGAAT+AGG - chr2_1:12283965-12283984 Msa0195440:intron 30.0%
AAGTTAGGCTTGTAATATAC+TGG + chr2_1:12284070-12284089 None:intergenic 30.0%
TTCTGTTATATTACAGCAAC+AGG - chr2_1:12284101-12284120 Msa0195440:CDS 30.0%
! TACTAGTAGTTTTCATTTCG+TGG - chr2_1:12284352-12284371 Msa0195440:CDS 30.0%
TCTAATCATGATCGCAATTA+CGG + chr2_1:12284444-12284463 None:intergenic 30.0%
GGAAAGAAAAGAAAAATCAC+TGG - chr2_1:12284706-12284725 Msa0195440:intron 30.0%
! AAGGTCTTCCTAATCATTTT+CGG - chr2_1:12284777-12284796 Msa0195440:intron 30.0%
TCAAACTTTATTATGCCTCA+AGG + chr2_1:12284905-12284924 None:intergenic 30.0%
GGTCTATATTCTATAGACTA+AGG - chr2_1:12284980-12284999 Msa0195440:intron 30.0%
GGCATTTCCTAATTCTTATA+TGG + chr2_1:12285055-12285074 None:intergenic 30.0%
ATCTTTGCTGCAATTAAGAT+AGG + chr2_1:12285336-12285355 None:intergenic 30.0%
TATCTTAATTGCAGCAAAGA+TGG - chr2_1:12285335-12285354 Msa0195440:intron 30.0%
ATCTTAATTGCAGCAAAGAT+GGG - chr2_1:12285336-12285355 Msa0195440:intron 30.0%
! GAACAAGAATGTTGTTCTTT+TGG - chr2_1:12285425-12285444 Msa0195440:intron 30.0%
! TTCTTTTGGCCATAGAAAAT+AGG - chr2_1:12285439-12285458 Msa0195440:intron 30.0%
!! TTTCGTCAAGTTGATATCAA+TGG - chr2_1:12285516-12285535 Msa0195440:CDS 30.0%
! TTAATCAATGTCTCGTTTTG+TGG + chr2_1:12285763-12285782 None:intergenic 30.0%
! ACAAAACGAGACATTGATTA+AGG - chr2_1:12285762-12285781 Msa0195440:CDS 30.0%
! ATGTTTTGTCTTGCATAAAG+TGG - chr2_1:12285897-12285916 Msa0195440:intron 30.0%
! AAGTGGATTTCATTGTAATC+TGG - chr2_1:12285914-12285933 Msa0195440:intron 30.0%
!! TTTTTGAGGGAAAAGTAAAG+TGG - chr2_1:12286012-12286031 Msa0195440:intron 30.0%
TAACGTTCATAGAAATTAGG+TGG + chr2_1:12286137-12286156 None:intergenic 30.0%
! TTACTACCTTCTTTTACAAG+TGG + chr2_1:12286209-12286228 None:intergenic 30.0%
ATAAACTGCAAATCTCATCT+GGG + chr2_1:12286579-12286598 None:intergenic 30.0%
CATTGTTAGGAGATATCTAA+GGG + chr2_1:12286794-12286813 None:intergenic 30.0%
TCATTGTTAGGAGATATCTA+AGG + chr2_1:12286795-12286814 None:intergenic 30.0%
ACACACAATGTTCTAACCAT+TGG - chr2_1:12282675-12282694 Msa0195440:three_prime_UTR 35.0%
! TGGTTAGCTAGCTCTTAAAA+TGG - chr2_1:12282704-12282723 Msa0195440:intron 35.0%
TGAGAAAAACCCTGAAGTAT+TGG - chr2_1:12282797-12282816 Msa0195440:three_prime_UTR 35.0%
AGGTTGTCACAAAAACTGTT+TGG + chr2_1:12282878-12282897 None:intergenic 35.0%
!! CCTTTGAGTTTTGCATTCTT+AGG + chr2_1:12282938-12282957 None:intergenic 35.0%
CCTAAGAATGCAAAACTCAA+AGG - chr2_1:12282935-12282954 Msa0195440:CDS 35.0%
TTTCGATAGGAAATGAGGAT+GGG + chr2_1:12283032-12283051 None:intergenic 35.0%
ATTTCGATAGGAAATGAGGA+TGG + chr2_1:12283033-12283052 None:intergenic 35.0%
CGAAATTTCGATAGGAAATG+AGG + chr2_1:12283037-12283056 None:intergenic 35.0%
! GATGCTTTCTTTTGTCTTCA+TGG - chr2_1:12283101-12283120 Msa0195440:CDS 35.0%
! AGTCATCATCTTTGTTGTGT+TGG + chr2_1:12283130-12283149 None:intergenic 35.0%
ACCATATCCAGTGAATACTT+TGG - chr2_1:12283191-12283210 Msa0195440:CDS 35.0%
! ACCAAAGTATTCACTGGATA+TGG + chr2_1:12283195-12283214 None:intergenic 35.0%
! ACACTAACCAAAGTATTCAC+TGG + chr2_1:12283201-12283220 None:intergenic 35.0%
GGTTTGGCACTTCAAATAAT+TGG - chr2_1:12283397-12283416 Msa0195440:CDS 35.0%
TTTACAGCATTCACAAGCTT+TGG + chr2_1:12283430-12283449 None:intergenic 35.0%
TGTAAGTAGCATCACTTACA+AGG - chr2_1:12283547-12283566 Msa0195440:intron 35.0%
AAGAAACAACCAAGTAACGT+GGG - chr2_1:12283692-12283711 Msa0195440:intron 35.0%
CATCACTATCCATTGAACTA+TGG - chr2_1:12283931-12283950 Msa0195440:intron 35.0%
ATGCATGTTCCATAGTTCAA+TGG + chr2_1:12283943-12283962 None:intergenic 35.0%
TCTATGTAAACGTACAGTAG+AGG + chr2_1:12284505-12284524 None:intergenic 35.0%
TCATTGCAAGGATTAGAAGT+CGG + chr2_1:12284758-12284777 None:intergenic 35.0%
ACTTCTAATCCTTGCAATGA+AGG - chr2_1:12284758-12284777 Msa0195440:intron 35.0%
!! TTCCTAATCATTTTCGGTGT+CGG - chr2_1:12284783-12284802 Msa0195440:intron 35.0%
ACAATGAGAAGACTAATGCA+TGG + chr2_1:12284820-12284839 None:intergenic 35.0%
!! GCATTAGTCTTCTCATTGTT+AGG - chr2_1:12284821-12284840 Msa0195440:intron 35.0%
AAGGACGACAAAGATATTGA+AGG + chr2_1:12284855-12284874 None:intergenic 35.0%
!!! GTTTTTGGTTTTAGTCCTTG+AGG - chr2_1:12284887-12284906 Msa0195440:CDS 35.0%
!! TAACTGAATGGCATTGACAT+AGG - chr2_1:12285090-12285109 Msa0195440:intron 35.0%
AATTCTTCAACATGAGGACT+TGG + chr2_1:12285228-12285247 None:intergenic 35.0%
AGTCCTCATGTTGAAGAATT+TGG - chr2_1:12285228-12285247 Msa0195440:intron 35.0%
AGTTATTGAGAAGAGGAGAA+TGG - chr2_1:12285305-12285324 Msa0195440:intron 35.0%
! ATGAGGTTGCCTATTTTCTA+TGG + chr2_1:12285451-12285470 None:intergenic 35.0%
TGTAATCAGATTCGCAAAGT+TGG + chr2_1:12285731-12285750 None:intergenic 35.0%
AATTTGAGTCAAGTTCCACT+CGG - chr2_1:12285785-12285804 Msa0195440:intron 35.0%
!!! ATATAGTATTTTGGTCCGAG+TGG + chr2_1:12285803-12285822 None:intergenic 35.0%
TTCATAGAAATTAGGTGGCA+TGG + chr2_1:12286132-12286151 None:intergenic 35.0%
AAACAAAATAAGGGCTGTGA+CGG + chr2_1:12286411-12286430 None:intergenic 35.0%
TTCTTTCCATACTCACATCT+CGG - chr2_1:12286429-12286448 Msa0195440:intron 35.0%
TTACCAAGAAAGCTTCTTGT+AGG - chr2_1:12286479-12286498 Msa0195440:CDS 35.0%
TACCAAGAAAGCTTCTTGTA+GGG - chr2_1:12286480-12286499 Msa0195440:CDS 35.0%
GATAAACTGCAAATCTCATC+TGG + chr2_1:12286580-12286599 None:intergenic 35.0%
AGACATGTTGCAGCATATAT+GGG + chr2_1:12286602-12286621 None:intergenic 35.0%
AACTCAGATCAAAGTACAGA+GGG + chr2_1:12286655-12286674 None:intergenic 35.0%
AAACTCAGATCAAAGTACAG+AGG + chr2_1:12286656-12286675 None:intergenic 35.0%
TCTGTACTTTGATCTGAGTT+TGG - chr2_1:12286655-12286674 Msa0195440:CDS 35.0%
AGGCAATATGCAACTTTATG+AGG - chr2_1:12286863-12286882 Msa0195440:CDS 35.0%
GTTCTAACCATTGGAAAGCT+TGG - chr2_1:12282684-12282703 Msa0195440:three_prime_UTR 40.0%
TAGCTAACCAAGCTTTCCAA+TGG + chr2_1:12282694-12282713 None:intergenic 40.0%
TTGTTTAACCATGCCATGAG+AGG - chr2_1:12282747-12282766 Msa0195440:three_prime_UTR 40.0%
TGTTTAACCATGCCATGAGA+GGG - chr2_1:12282748-12282767 Msa0195440:three_prime_UTR 40.0%
!! GAAAAACCCTGAAGTATTGG+AGG - chr2_1:12282800-12282819 Msa0195440:three_prime_UTR 40.0%
TCTTTGCCTCCAATACTTCA+GGG + chr2_1:12282809-12282828 None:intergenic 40.0%
ATCTTTGCCTCCAATACTTC+AGG + chr2_1:12282810-12282829 None:intergenic 40.0%
ACATAAGCCAAGATATGTGC+AGG - chr2_1:12282907-12282926 Msa0195440:intron 40.0%
TTCGCCTTCGAAATTTCGAT+AGG + chr2_1:12283045-12283064 None:intergenic 40.0%
GTGAATGCTGTAAACCATGA+TGG - chr2_1:12283436-12283455 Msa0195440:CDS 40.0%
TTCCTAGCAAGAATATGCAG+AGG + chr2_1:12283469-12283488 None:intergenic 40.0%
! GCTAGGAAGCCTAATTGTTT+TGG - chr2_1:12283481-12283500 Msa0195440:CDS 40.0%
GTGCAGCTTCCAAAACAATT+AGG + chr2_1:12283493-12283512 None:intergenic 40.0%
!!! ATTGTTTTGGAAGCTGCACA+AGG - chr2_1:12283494-12283513 Msa0195440:CDS 40.0%
CAAGAAACAACCAAGTAACG+TGG - chr2_1:12283691-12283710 Msa0195440:intron 40.0%
TATTATGGACCCACGTTACT+TGG + chr2_1:12283704-12283723 None:intergenic 40.0%
! AAGGTGTTTGTGTCAATGTC+AGG - chr2_1:12283847-12283866 Msa0195440:intron 40.0%
TAGTCTGAGTTTGTGCTTGA+AGG + chr2_1:12283909-12283928 None:intergenic 40.0%
! AACCATTGCTCGCGAAATTT+TGG - chr2_1:12284161-12284180 Msa0195440:CDS 40.0%
TACCAAAATTTCGCGAGCAA+TGG + chr2_1:12284166-12284185 None:intergenic 40.0%
CTAGCAACATGGTTTACAAG+AGG + chr2_1:12284198-12284217 None:intergenic 40.0%
AAGGATTAGAAGTCGGATCA+TGG + chr2_1:12284751-12284770 None:intergenic 40.0%
TTAGGAAGACCTTCATTGCA+AGG + chr2_1:12284770-12284789 None:intergenic 40.0%
TACCGACACCGAAAATGATT+AGG + chr2_1:12284788-12284807 None:intergenic 40.0%
CATTGACATAGGAGCAACAT+GGG - chr2_1:12285101-12285120 Msa0195440:intron 40.0%
CATGGGTAGAGAAAATCCAA+AGG - chr2_1:12285118-12285137 Msa0195440:intron 40.0%
AGAGAAAATCCAAAGGAGGA+AGG - chr2_1:12285125-12285144 Msa0195440:intron 40.0%
! TGTGTTTTTCCTTCCTCCTT+TGG + chr2_1:12285137-12285156 None:intergenic 40.0%
GGAGGAAGGAAAAACACATT+CGG - chr2_1:12285139-12285158 Msa0195440:intron 40.0%
GAGGAAGGAAAAACACATTC+GGG - chr2_1:12285140-12285159 Msa0195440:intron 40.0%
CCACCAAATTCTTCAACATG+AGG + chr2_1:12285234-12285253 None:intergenic 40.0%
CCTCATGTTGAAGAATTTGG+TGG - chr2_1:12285231-12285250 Msa0195440:intron 40.0%
! CATGTTGAAGAATTTGGTGG+TGG - chr2_1:12285234-12285253 Msa0195440:intron 40.0%
GATTCTAGATATGTACCCTG+TGG - chr2_1:12285380-12285399 Msa0195440:intron 40.0%
! TGCATCTTGCTGCTACTTAT+AGG - chr2_1:12285550-12285569 Msa0195440:intron 40.0%
! ATCTTGCTGCTACTTATAGG+AGG - chr2_1:12285553-12285572 Msa0195440:intron 40.0%
CTTATAGGAGGTTTAAGCCT+TGG - chr2_1:12285565-12285584 Msa0195440:intron 40.0%
AAATGCAGTGGGAATACAAG+TGG - chr2_1:12285610-12285629 Msa0195440:intron 40.0%
! TCAATGTCTCGTTTTGTGGA+TGG + chr2_1:12285759-12285778 None:intergenic 40.0%
! TGAGCAAGATTCTGCAGTTT+TGG + chr2_1:12286240-12286259 None:intergenic 40.0%
! TTTTAGCAGTAGTGGTATGC+CGG + chr2_1:12286336-12286355 None:intergenic 40.0%
ACAGCATAGAGTTTGAAAGC+AGG + chr2_1:12286362-12286381 None:intergenic 40.0%
TTCAAACCGAGATGTGAGTA+TGG + chr2_1:12286438-12286457 None:intergenic 40.0%
AACCCTACAAGAAGCTTTCT+TGG + chr2_1:12286485-12286504 None:intergenic 40.0%
CACATCTATAGCATCTGTTC+TGG - chr2_1:12286517-12286536 Msa0195440:CDS 40.0%
CAGACATGTTGCAGCATATA+TGG + chr2_1:12286603-12286622 None:intergenic 40.0%
ATCTGAGTTTGGCTATGTGT+CGG - chr2_1:12286666-12286685 Msa0195440:CDS 40.0%
GTCTGCAAGTGTTCATTGTT+AGG + chr2_1:12286807-12286826 None:intergenic 40.0%
GTGCACAATGAGAACCAAAA+GGG + chr2_1:12286889-12286908 None:intergenic 40.0%
AGTGCACAATGAGAACCAAA+AGG + chr2_1:12286890-12286909 None:intergenic 40.0%
GGATGTTCCTGCACATATCT+TGG + chr2_1:12282917-12282936 None:intergenic 45.0%
TTCATGTTGCAGCAGAGCTT+GGG - chr2_1:12282970-12282989 Msa0195440:CDS 45.0%
AATGAGGATGGGATCTCTTC+TGG + chr2_1:12283021-12283040 None:intergenic 45.0%
! AGGCGAAACTCCTCTTTTCT+TGG - chr2_1:12283058-12283077 Msa0195440:CDS 45.0%
TGAACAGCTGCCAAGAAAAG+AGG + chr2_1:12283071-12283090 None:intergenic 45.0%
! CTTTTCTTGGCAGCTGTTCA+TGG - chr2_1:12283071-12283090 Msa0195440:CDS 45.0%
GCAGAGGTGATAATCCATCA+TGG + chr2_1:12283453-12283472 None:intergenic 45.0%
CACCTCTGCATATTCTTGCT+AGG - chr2_1:12283464-12283483 Msa0195440:CDS 45.0%
!! TTTTGGAAGCTGCACAAGGA+TGG - chr2_1:12283498-12283517 Msa0195440:CDS 45.0%
! AGAAGGTAGGACTAGCAACA+TGG + chr2_1:12284209-12284228 None:intergenic 45.0%
TCATGGATTCCCAATTGGGT+GGG + chr2_1:12284734-12284753 None:intergenic 45.0%
ATCATGGATTCCCAATTGGG+TGG + chr2_1:12284735-12284754 None:intergenic 45.0%
CGGATCATGGATTCCCAATT+GGG + chr2_1:12284738-12284757 None:intergenic 45.0%
TCGGATCATGGATTCCCAAT+TGG + chr2_1:12284739-12284758 None:intergenic 45.0%
! GCATTGACATAGGAGCAACA+TGG - chr2_1:12285100-12285119 Msa0195440:intron 45.0%
GGGTAGAGAAAATCCAAAGG+AGG - chr2_1:12285121-12285140 Msa0195440:intron 45.0%
!! GTTGAAGAATTTGGTGGTGG+TGG - chr2_1:12285237-12285256 Msa0195440:intron 45.0%
CCACATCATGAATAGCCACA+GGG + chr2_1:12285398-12285417 None:intergenic 45.0%
TCCACATCATGAATAGCCAC+AGG + chr2_1:12285399-12285418 None:intergenic 45.0%
GTGGGAATACAAGTGGTACA+AGG - chr2_1:12285617-12285636 Msa0195440:intron 45.0%
CACGCACCACTTGTAAAAGA+AGG - chr2_1:12286200-12286219 Msa0195440:CDS 45.0%
AACTGCAGAATCTTGCTCAG+TGG - chr2_1:12286241-12286260 Msa0195440:CDS 45.0%
!!! GCAGGCTTTTTTAGCAGTAG+TGG + chr2_1:12286344-12286363 None:intergenic 45.0%
ACCAAGGCAAAAAAGGACAC+AGG + chr2_1:12286626-12286645 None:intergenic 45.0%
GAGTTGGACCAAGGCAAAAA+AGG + chr2_1:12286633-12286652 None:intergenic 45.0%
GATCAAAGTACAGAGGGAGT+TGG + chr2_1:12286649-12286668 None:intergenic 45.0%
! GCAACTTTATGAGGCCCTTT+TGG - chr2_1:12286872-12286891 Msa0195440:CDS 45.0%
!!! TATATATATATATAGTATTT+TGG + chr2_1:12285812-12285831 None:intergenic 5.0%
!!! TTATTTTTTTATTTTTTTTG+AGG - chr2_1:12285998-12286017 Msa0195440:intron 5.0%
!!! TATTTTTTTATTTTTTTTGA+GGG - chr2_1:12285999-12286018 Msa0195440:intron 5.0%
TTCTCTCCATTGCCCTCTCA+TGG + chr2_1:12282763-12282782 None:intergenic 50.0%
CTCTGCTGCAACATGAAGAG+GGG + chr2_1:12282967-12282986 None:intergenic 50.0%
GCTCTGCTGCAACATGAAGA+GGG + chr2_1:12282968-12282987 None:intergenic 50.0%
AGCTCTGCTGCAACATGAAG+AGG + chr2_1:12282969-12282988 None:intergenic 50.0%
CTTCATGTTGCAGCAGAGCT+TGG - chr2_1:12282969-12282988 Msa0195440:CDS 50.0%
AGTCCTACCTTCTAACCCAC+CGG - chr2_1:12284216-12284235 Msa0195440:CDS 50.0%
ATGTAGAGCAAGCGCAAGCA+AGG - chr2_1:12284685-12284704 Msa0195440:intron 50.0%
!! ATCACTGGTTCCCACCCAAT+TGG - chr2_1:12284721-12284740 Msa0195440:intron 50.0%
!! TCACTGGTTCCCACCCAATT+GGG - chr2_1:12284722-12284741 Msa0195440:intron 50.0%
!! GAAGAATTTGGTGGTGGTGG+TGG - chr2_1:12285240-12285259 Msa0195440:intron 50.0%
CCCTGTGGCTATTCATGATG+TGG - chr2_1:12285395-12285414 Msa0195440:intron 50.0%
TGCTGCGATGCAAATGCAGT+GGG - chr2_1:12285599-12285618 Msa0195440:intron 50.0%
CAAGTGGTGCGTGTGTATCA+AGG + chr2_1:12286193-12286212 None:intergenic 50.0%
ACAACCTCAACAGCTATCCC+AGG - chr2_1:12286293-12286312 Msa0195440:intron 50.0%
ATTCTTGATCAGGACCGCCT+GGG + chr2_1:12286313-12286332 None:intergenic 50.0%
GGTATGCCGGATTCTTGATC+AGG + chr2_1:12286323-12286342 None:intergenic 50.0%
CAAAATAAGGGCTGTGACGG+AGG + chr2_1:12286408-12286427 None:intergenic 50.0%
GTTCTGGTCTCATTCTGTGC+AGG - chr2_1:12286533-12286552 Msa0195440:CDS 50.0%
!!! GCCTGTGTCCTTTTTTGCCT+TGG - chr2_1:12286622-12286641 Msa0195440:CDS 50.0%
GAGTTTGGCTATGTGTCGGA+AGG - chr2_1:12286670-12286689 Msa0195440:CDS 50.0%
AACACTTTGTAGCTGCGCTG+AGG + chr2_1:12286698-12286717 None:intergenic 50.0%
ACCATGCCATGAGAGGGCAA+TGG - chr2_1:12282754-12282773 Msa0195440:three_prime_UTR 55.0%
TCCATTGCCCTCTCATGGCA+TGG + chr2_1:12282758-12282777 None:intergenic 55.0%
GTCCTACCTTCTAACCCACC+GGG - chr2_1:12284217-12284236 Msa0195440:CDS 55.0%
CCAACTACTTGCGCTAGACC+CGG + chr2_1:12284238-12284257 None:intergenic 55.0%
!! GAATTTGGTGGTGGTGGTGG+TGG - chr2_1:12285243-12285262 Msa0195440:intron 55.0%
TTTAAGCCTTGGCGTGTGCC+TGG - chr2_1:12285576-12285595 Msa0195440:intron 55.0%
TGCATTTGCATCGCAGCACC+AGG + chr2_1:12285597-12285616 None:intergenic 55.0%
GTGCTGCGATGCAAATGCAG+TGG - chr2_1:12285598-12285617 Msa0195440:intron 55.0%
ACCTCAACAGCTATCCCAGG+CGG - chr2_1:12286296-12286315 Msa0195440:intron 55.0%
GATTCTTGATCAGGACCGCC+TGG + chr2_1:12286314-12286333 None:intergenic 55.0%
GGCGGTCCTGATCAAGAATC+CGG - chr2_1:12286314-12286333 Msa0195440:intron 55.0%
GTACAGAGGGAGTTGGACCA+AGG + chr2_1:12286642-12286661 None:intergenic 55.0%
!! GACCCGGTGGGTTAGAAGGT+AGG + chr2_1:12284222-12284241 None:intergenic 60.0%
!! GCTAGACCCGGTGGGTTAGA+AGG + chr2_1:12284226-12284245 None:intergenic 60.0%
CTACTTGCGCTAGACCCGGT+GGG + chr2_1:12284234-12284253 None:intergenic 60.0%
ACTACTTGCGCTAGACCCGG+TGG + chr2_1:12284235-12284254 None:intergenic 60.0%
CCGGGTCTAGCGCAAGTAGT+TGG - chr2_1:12284235-12284254 Msa0195440:CDS 60.0%
!! TTTGGTGGTGGTGGTGGTGG+TGG - chr2_1:12285246-12285265 Msa0195440:intron 60.0%
ACCGCCTGGGATAGCTGTTG+AGG + chr2_1:12286300-12286319 None:intergenic 60.0%
!! GGTGGTGGTGGTGGTGGCAT+AGG - chr2_1:12285255-12285274 Msa0195440:intron 65.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG - chr2_1:12285249-12285268 Msa0195440:intron 70.0%
GCAGCACCAGGCACACGCCA+AGG + chr2_1:12285585-12285604 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 12282669 12286991 12282669 ID=Msa0195440;Name=Msa0195440
chr2_1 mRNA 12282669 12286991 12282669 ID=Msa0195440-mRNA-1;Parent=Msa0195440;Name=Msa0195440-mRNA-1;_AED=0.07;_eAED=0.07;_QI=54|1|1|1|0.75|0.66|9|215|697
chr2_1 exon 12282669 12282714 12282669 ID=Msa0195440-mRNA-1:exon:5364;Parent=Msa0195440-mRNA-1
chr2_1 exon 12282740 12282886 12282740 ID=Msa0195440-mRNA-1:exon:5363;Parent=Msa0195440-mRNA-1
chr2_1 exon 12282911 12283544 12282911 ID=Msa0195440-mRNA-1:exon:5362;Parent=Msa0195440-mRNA-1
chr2_1 exon 12284022 12284548 12284022 ID=Msa0195440-mRNA-1:exon:5361;Parent=Msa0195440-mRNA-1
chr2_1 exon 12284856 12284969 12284856 ID=Msa0195440-mRNA-1:exon:5360;Parent=Msa0195440-mRNA-1
chr2_1 exon 12285478 12285543 12285478 ID=Msa0195440-mRNA-1:exon:5359;Parent=Msa0195440-mRNA-1
chr2_1 exon 12285657 12285791 12285657 ID=Msa0195440-mRNA-1:exon:5358;Parent=Msa0195440-mRNA-1
chr2_1 exon 12286113 12286262 12286113 ID=Msa0195440-mRNA-1:exon:5357;Parent=Msa0195440-mRNA-1
chr2_1 exon 12286448 12286991 12286448 ID=Msa0195440-mRNA-1:exon:5356;Parent=Msa0195440-mRNA-1
chr2_1 five_prime_UTR 12286938 12286991 12286938 ID=Msa0195440-mRNA-1:five_prime_utr;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12286448 12286937 12286448 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12286113 12286262 12286113 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12285657 12285791 12285657 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12285478 12285543 12285478 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12284856 12284969 12284856 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12284022 12284548 12284022 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 CDS 12282933 12283544 12282933 ID=Msa0195440-mRNA-1:cds;Parent=Msa0195440-mRNA-1
chr2_1 three_prime_UTR 12282911 12282932 12282911 ID=Msa0195440-mRNA-1:three_prime_utr;Parent=Msa0195440-mRNA-1
chr2_1 three_prime_UTR 12282740 12282886 12282740 ID=Msa0195440-mRNA-1:three_prime_utr;Parent=Msa0195440-mRNA-1
chr2_1 three_prime_UTR 12282669 12282714 12282669 ID=Msa0195440-mRNA-1:three_prime_utr;Parent=Msa0195440-mRNA-1
Gene Sequence

>Msa0195440

ATGGCTGAGATTGAGTTTGAAAGTTTGTTTAACCATGCCATGAGAGGGCAATGGAGAGAAGTTTTAGAGTCGTATGAGAAAAACCCTGAAGTATTGGAGGCAAAGATCACAAAAGCAGAAGACACAGTGCTACACATTGCTATATACGTGAGCCAAACAGTTTTTGTGACAACCTTGTTAGATAACATAAGCCAAGATATGTGCAGGAACATCCTAAGAATGCAAAACTCAAAGGGAAATACCCCTCTTCATGTTGCAGCAGAGCTTGGGAATGTAGACATTTGTAACAACATAGCCAGAAGAGATCCCATCCTCATTTCCTATCGAAATTTCGAAGGCGAAACTCCTCTTTTCTTGGCAGCTGTTCATGGAAAAAGAGATGCTTTCTTTTGTCTTCATGGTCGCCAACACAACAAAGATGATGACTCACTTTCAATCAAAAACAATGGAGACACTATTCTTCATTCTACCATATCCAGTGAATACTTTGGTTTGGCACTTCAAATAATTGGAATGTATCCAAAGCTTGTGAATGCTGTAAACCATGATGGATTATCACCTCTGCATATTCTTGCTAGGAAGCCTAATTGTTTTGGAAGCTGCACAAGGATGGATCTAATTGATCGTATCATCTACACTTGTTCAATTGTTGATGAGAACAAGGAAGAAAGATATGACCTTCAAGCACAAACTCAGACTACTCATCACTATCCATTGAACTATGGAACATGCATGACCTTCATATCTTTGTTGAATAGGTTTTTTAAAGTAACAACAACAGGAAAAGATACAAATGCTGCTGCAACTAGTGACGAAGAGAACCATTGCTCGCGAAATTTTGAGCAAGCGCAAGCAAGGAAAGAAAAGAAAAATCACTGGTTCCCACCCAATTGGGAATCCATGATCCGACTTCTAATCCTTGCAATGAAGGTCTTCCTAATCATTTTCGGTGTCGGAGCAACATGGGTAGAGAAAATCCAAAGGAGGAAGGAAAAACACATTCGGGCTACACAAGTAATGAATGAACTGATACAACGCGCTTCCTTATACAAGTATGATTTCACAGGACCAAGTCCTCATGTTGAAGAATTTGGTGGTGGTGGTGGTGGTGGTGGCATAGGCAAAATCAAGAGCAATACAGAAAATGAAGTTATTGAGAAGAGGAGAATGGTGTCACCTATCTTAATTGCAGCAAAGATGGGAGTGACTGAAATGATTGAAAAGATTCTAGATATGTACCCTGTGGCTATTCATGATGTGGACAGTCAGAACAAGAATGTTGTTCTTTTGGCCATAGAAAATAGGCAACCTCATGTATACAGTTTGTTAAATAAGAGGAGTGTAATCAAAGAGACTGCATTTCGTCAAGTTGATATCAATGGTAACAGTGCATTGCATCTTGCTGCTACTTATAGGAGGTTTAAGCCTTGGCGTGTGCCTGGTGCTGCGATGCAAATGCAGTGGGAATACAAGTGGTACAAGCTTGTTAAGGACTCCATGCCACCTAATTTCTATGAACGTTATAATAAGGATGGGAAAACAGCAAAGCAAGTCTTCCTTGATACACACGCACCACTTGTAAAAGAAGGTAGTAAATGGCTCACCAAAACTGCAGAATCTTGCTCAGTGGTAGCAGCACTTGTTGCAACTGTTGCATTCACAACCTCAACAGCTATCCCAGGCGGTCCTGATCAAGAATCCGGCATACCACTACTGCTAAAAAAGCCTGCTTTCAAACTCTATGCTGTAGCATCACTTGTTGCACTTTGCTCCTCCGTCACAGCCCTTATTTTGTTTCTTTCCATACTCACATCTCGGTTTGAAGAAAAAGATTTTGTTATCGACTTACCAAGAAAGCTTCTTGTAGGGTTGACTACACTCTTCACATCTATAGCATCTGTTCTGGTCTCATTCTGTGCAGGACATTTCTTTATTGTTGAAGCCCAGATGAGATTTGCAGTTTATCCCATATATGCTGCAACATGTCTGCCTGTGTCCTTTTTTGCCTTGGTCCAACTCCCTCTGTACTTTGATCTGAGTTTGGCTATGTGTCGGAAGGTTCCTCAGCGCAGCTACAAAGTGTTCTTTCACTAG
Protein sequence

>Msa0195440

MAEIEFESLFNHAMRGQWREVLESYEKNPEVLEAKITKAEDTVLHIAIYVSQTVFVTTLLDNISQDMCRNILRMQNSKGNTPLHVAAELGNVDICNNIARRDPILISYRNFEGETPLFLAAVHGKRDAFFCLHGRQHNKDDDSLSIKNNGDTILHSTISSEYFGLALQIIGMYPKLVNAVNHDGLSPLHILARKPNCFGSCTRMDLIDRIIYTCSIVDENKEERYDLQAQTQTTHHYPLNYGTCMTFISLLNRFFKVTTTGKDTNAAATSDEENHCSRNFEQAQARKEKKNHWFPPNWESMIRLLILAMKVFLIIFGVGATWVEKIQRRKEKHIRATQVMNELIQRASLYKYDFTGPSPHVEEFGGGGGGGGIGKIKSNTENEVIEKRRMVSPILIAAKMGVTEMIEKILDMYPVAIHDVDSQNKNVVLLAIENRQPHVYSLLNKRSVIKETAFRQVDINGNSALHLAATYRRFKPWRVPGAAMQMQWEYKWYKLVKDSMPPNFYERYNKDGKTAKQVFLDTHAPLVKEGSKWLTKTAESCSVVAALVATVAFTTSTAIPGGPDQESGIPLLLKKPAFKLYAVASLVALCSSVTALILFLSILTSRFEEKDFVIDLPRKLLVGLTTLFTSIASVLVSFCAGHFFIVEAQMRFAVYPIYAATCLPVSFFALVQLPLYFDLSLAMCRKVPQRSYKVFFH*