Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385370 | YP_009141600.1 | 100.000 | 201 | 0 | 0 | 1 | 201 | 1 | 201 | 3.55e-143 | 407 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385370 | sp|Q9BBT5|RR4_LOTJA | 95.522 | 201 | 9 | 0 | 1 | 201 | 1 | 201 | 8.95e-140 | 391 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385370 | A0A0F6NLD0 | 100.000 | 201 | 0 | 0 | 1 | 201 | 1 | 201 | 1.70e-143 | 407 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1354350 | Msa1385370 | 0.866030 | 3.840245e-65 | -8.615850e-47 |
| Msa1354380 | Msa1385370 | 0.828018 | 1.135011e-54 | -8.615850e-47 |
| Msa1354390 | Msa1385370 | 0.842461 | 2.549659e-58 | -8.615850e-47 |
| Msa1354900 | Msa1385370 | 0.818511 | 1.886939e-52 | -8.615850e-47 |
| Msa1354930 | Msa1385370 | 0.809170 | 2.159552e-50 | -8.615850e-47 |
| Msa1354950 | Msa1385370 | 0.871187 | 8.269260e-67 | -8.615850e-47 |
| Msa1366010 | Msa1385370 | 0.864178 | 1.466159e-64 | -8.615850e-47 |
| Msa1366050 | Msa1385370 | 0.856578 | 2.926848e-62 | -8.615850e-47 |
| Msa1368270 | Msa1385370 | 0.863464 | 2.444156e-64 | -8.615850e-47 |
| Msa1368290 | Msa1385370 | 0.854544 | 1.147147e-61 | -8.615850e-47 |
| Msa1368300 | Msa1385370 | 0.833625 | 4.790663e-56 | -8.615850e-47 |
| Msa1380590 | Msa1385370 | 0.807326 | 5.339016e-50 | -8.615850e-47 |
| Msa1380640 | Msa1385370 | 0.810646 | 1.039466e-50 | -8.615850e-47 |
| Msa1380710 | Msa1385370 | 0.807023 | 6.189216e-50 | -8.615850e-47 |
| Msa1385370 | Msa1394230 | 0.802106 | 6.566133e-49 | -8.615850e-47 |
| Msa1385370 | Msa1398700 | 0.837933 | 3.878592e-57 | -8.615850e-47 |
| Msa1385370 | Msa1398710 | 0.817838 | 2.679673e-52 | -8.615850e-47 |
| Msa1385370 | Msa1398720 | 0.824530 | 7.680967e-54 | -8.615850e-47 |
| Msa1269400 | Msa1385370 | 0.819746 | 9.877062e-53 | -8.615850e-47 |
| Msa1269420 | Msa1385370 | 0.836703 | 8.009303e-57 | -8.615850e-47 |
| Msa1305090 | Msa1385370 | 0.802256 | 6.116460e-49 | -8.615850e-47 |
| Msa1086180 | Msa1385370 | 0.807371 | 5.223863e-50 | -8.615850e-47 |
| Msa1086280 | Msa1385370 | 0.804721 | 1.885832e-49 | -8.615850e-47 |
| Msa1086450 | Msa1385370 | 0.802227 | 6.200691e-49 | -8.615850e-47 |
| Msa0268410 | Msa1385370 | 0.807153 | 5.808562e-50 | -8.615850e-47 |
| Msa0268550 | Msa1385370 | 0.804563 | 2.034148e-49 | -8.615850e-47 |
| Msa0268580 | Msa1385370 | 0.817260 | 3.615612e-52 | -8.615850e-47 |
| Msa0268640 | Msa1385370 | 0.800342 | 1.507756e-48 | -8.615850e-47 |
| Msa0268940 | Msa1385370 | 0.800850 | 1.187782e-48 | -8.615850e-47 |
| Msa0269050 | Msa1385370 | 0.800055 | 1.724281e-48 | -8.615850e-47 |
| Msa0344190 | Msa1385370 | 0.805753 | 1.146273e-49 | -8.615850e-47 |
| Msa0344300 | Msa1385370 | 0.803710 | 3.060831e-49 | -8.615850e-47 |
| Msa1143130 | Msa1385370 | 0.801782 | 7.652290e-49 | -8.615850e-47 |
| Msa0483990 | Msa1385370 | 0.827762 | 1.307835e-54 | -8.615850e-47 |
| Msa0582520 | Msa1385370 | 0.804479 | 2.118521e-49 | -8.615850e-47 |
| Msa0582700 | Msa1385370 | 0.806748 | 7.075885e-50 | -8.615850e-47 |
| Msa0582780 | Msa1385370 | 0.806297 | 8.810374e-50 | -8.615850e-47 |
| Msa0867640 | Msa1385370 | 0.838212 | 3.287658e-57 | -8.615850e-47 |
| Msa0867650 | Msa1385370 | 0.809197 | 2.131811e-50 | -8.615850e-47 |
| Msa0931240 | Msa1385370 | 0.809175 | 2.154368e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385370 | MtrunA17_Chr7g0229301 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa1385370 | MtrunA17_Chr6g0454441 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa1385370 | MtrunA17_Chr4g0016451 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa1385370 | MtrunA17_Chr6g0466281 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa1385370 | MtrunA17_CPg0493311 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa1385370 | MtrunA17_Chr7g0229661 | 84.080 | 201 | 13 | 1 | 1 | 201 | 1 | 182 | 1.89e-115 | 327 |
| Msa1385370 | MtrunA17_Chr4g0015291 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 3.32e-108 | 307 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1385370 | ATCG00380.1 | 88.060 | 201 | 24 | 0 | 1 | 201 | 1 | 201 | 2.12e-126 | 355 |
Find 33 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAATAATAGATAGTAAA+TGG | 0.189787 | tig0020748:+39234 | Msa1385370:CDS |
| GATTGACTAATCCTTTATAT+TGG | 0.284280 | tig0020748:-39214 | None:intergenic |
| CTTGATCCATTCCAATATAA+AGG | 0.291752 | tig0020748:+39203 | Msa1385370:CDS |
| CTTAAATACGTTCGTATCGC+TGG | 0.342652 | tig0020748:+38903 | Msa1385370:CDS |
| ACAATTACTTGAAATGCGTT+TGG | 0.359489 | tig0020748:+38959 | Msa1385370:CDS |
| TTTGAAAATTAATGAATTGC+TGG | 0.365999 | tig0020748:+39262 | Msa1385370:CDS |
| ATGGATTGGCAATTCTTCGC+GGG | 0.450528 | tig0020748:-39174 | None:intergenic |
| TACCAGGACTAACTAGTAAA+CGG | 0.463029 | tig0020748:+38760 | Msa1385370:CDS |
| TGAGGTTTGCAACGATAACT+TGG | 0.468099 | tig0020748:-39083 | None:intergenic |
| ACTAATCCTTTATATTGGAA+TGG | 0.475526 | tig0020748:-39209 | None:intergenic |
| GTCTGTGGTTAACTAATTGA+CGG | 0.477609 | tig0020748:-39028 | None:intergenic |
| TCAAATAATAGATAGTAAAT+GGG | 0.487910 | tig0020748:+39235 | Msa1385370:CDS |
| ATAATAGATAGTAAATGGGT+TGG | 0.504615 | tig0020748:+39239 | Msa1385370:CDS |
| CTAAGTTCGCTTCCAACTGT+AGG | 0.508663 | tig0020748:-38783 | None:intergenic |
| GGATCAAGAGTCAAATGGAT+TGG | 0.511159 | tig0020748:-39188 | None:intergenic |
| GGAAAAGCGAAAGGTTCAAC+CGG | 0.516182 | tig0020748:+38924 | Msa1385370:CDS |
| TCTGTGGTTAACTAATTGAC+GGG | 0.521261 | tig0020748:-39027 | None:intergenic |
| GGCCGTTTACTAGTTAGTCC+TGG | 0.527000 | tig0020748:-38762 | None:intergenic |
| AATGGATTGGCAATTCTTCG+CGG | 0.545268 | tig0020748:-39175 | None:intergenic |
| TCAAGATATTATTACAGCGA+AGG | 0.559737 | tig0020748:+39103 | Msa1385370:CDS |
| TTCGCTGTAATAATATCTTG+AGG | 0.560954 | tig0020748:-39101 | None:intergenic |
| TAATTGTAGTAAAACTTGAC+CGG | 0.568250 | tig0020748:-38943 | None:intergenic |
| ACTAGTAAACGGCCTACAGT+TGG | 0.568372 | tig0020748:+38771 | Msa1385370:CDS |
| CAAGATATTATTACAGCGAA+GGG | 0.569002 | tig0020748:+39104 | Msa1385370:CDS |
| TTAACTAATTGACGGGCTTG+AGG | 0.581554 | tig0020748:-39020 | None:intergenic |
| GGAATGGATCAAGAGTCAAA+TGG | 0.582974 | tig0020748:-39193 | None:intergenic |
| GATTGGCAATTCTTCGCGGG+GGG | 0.584547 | tig0020748:-39171 | None:intergenic |
| TGGATTGGCAATTCTTCGCG+GGG | 0.610754 | tig0020748:-39173 | None:intergenic |
| TGTTTATGTCACGTTACAGA+GGG | 0.632465 | tig0020748:+38703 | None:intergenic |
| GTGTTTATGTCACGTTACAG+AGG | 0.633128 | tig0020748:+38702 | None:intergenic |
| CGTATCGCTGGAAAAGCGAA+AGG | 0.633501 | tig0020748:+38915 | Msa1385370:CDS |
| CATTAACTAAAACATGTCTG+TGG | 0.663264 | tig0020748:-39043 | None:intergenic |
| GGATTGGCAATTCTTCGCGG+GGG | 0.681198 | tig0020748:-39172 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAATAATAGATAGTAAA+TGG | + | tig0020748:39234-39253 | Msa1385370:CDS | 15.0% |
| !! | TCAAATAATAGATAGTAAAT+GGG | + | tig0020748:39235-39254 | Msa1385370:CDS | 15.0% |
| !! | TTTGAAAATTAATGAATTGC+TGG | + | tig0020748:39262-39281 | Msa1385370:CDS | 20.0% |
| !! | CAAAAATTGCGTTTTCATTA+TGG | + | tig0020748:38861-38880 | Msa1385370:CDS | 25.0% |
| ! | TAATTGTAGTAAAACTTGAC+CGG | - | tig0020748:38946-38965 | None:intergenic | 25.0% |
| !!! | GATAACATACTTTTTCGATT+GGG | + | tig0020748:38981-39000 | Msa1385370:CDS | 25.0% |
| ! | ACTAATCCTTTATATTGGAA+TGG | - | tig0020748:39212-39231 | None:intergenic | 25.0% |
| ! | GATTGACTAATCCTTTATAT+TGG | - | tig0020748:39217-39236 | None:intergenic | 25.0% |
| ! | ATAATAGATAGTAAATGGGT+TGG | + | tig0020748:39239-39258 | Msa1385370:CDS | 25.0% |
| TTTCAAAAAAATACGTCGTC+TGG | + | tig0020748:38731-38750 | Msa1385370:CDS | 30.0% | |
| TTCAAAAAAATACGTCGTCT+GGG | + | tig0020748:38732-38751 | Msa1385370:CDS | 30.0% | |
| ACAATTACTTGAAATGCGTT+TGG | + | tig0020748:38959-38978 | Msa1385370:CDS | 30.0% | |
| !!! | GGATAACATACTTTTTCGAT+TGG | + | tig0020748:38980-38999 | Msa1385370:CDS | 30.0% |
| CATTAACTAAAACATGTCTG+TGG | - | tig0020748:39046-39065 | None:intergenic | 30.0% | |
| !!! | CACAGACATGTTTTAGTTAA+TGG | + | tig0020748:39044-39063 | Msa1385370:CDS | 30.0% |
| TTCGCTGTAATAATATCTTG+AGG | - | tig0020748:39104-39123 | None:intergenic | 30.0% | |
| TCAAGATATTATTACAGCGA+AGG | + | tig0020748:39103-39122 | Msa1385370:CDS | 30.0% | |
| CAAGATATTATTACAGCGAA+GGG | + | tig0020748:39104-39123 | Msa1385370:CDS | 30.0% | |
| CTTGATCCATTCCAATATAA+AGG | + | tig0020748:39203-39222 | Msa1385370:CDS | 30.0% | |
| !!! | CGACGTATTTTTTTGAAACG+AGG | - | tig0020748:38729-38748 | None:intergenic | 35.0% |
| TCAAAAAAATACGTCGTCTG+GGG | + | tig0020748:38733-38752 | Msa1385370:CDS | 35.0% | |
| TACCAGGACTAACTAGTAAA+CGG | + | tig0020748:38760-38779 | Msa1385370:CDS | 35.0% | |
| !!! | CATACTTTTTCGATTGGGTA+TGG | + | tig0020748:38986-39005 | Msa1385370:CDS | 35.0% |
| TCTGTGGTTAACTAATTGAC+GGG | - | tig0020748:39030-39049 | None:intergenic | 35.0% | |
| GTCTGTGGTTAACTAATTGA+CGG | - | tig0020748:39031-39050 | None:intergenic | 35.0% | |
| CAAAAAAATACGTCGTCTGG+GGG | + | tig0020748:38734-38753 | Msa1385370:CDS | 40.0% | |
| !!! | ATTTTTTACTCGAGCGCGAT+TGG | - | tig0020748:38812-38831 | None:intergenic | 40.0% |
| CTTAAATACGTTCGTATCGC+TGG | + | tig0020748:38903-38922 | Msa1385370:CDS | 40.0% | |
| !! | TTAACTAATTGACGGGCTTG+AGG | - | tig0020748:39023-39042 | None:intergenic | 40.0% |
| TGAGGTTTGCAACGATAACT+TGG | - | tig0020748:39086-39105 | None:intergenic | 40.0% | |
| !! | AATGGATTGGCAATTCTTCG+CGG | - | tig0020748:39178-39197 | None:intergenic | 40.0% |
| GGATCAAGAGTCAAATGGAT+TGG | - | tig0020748:39191-39210 | None:intergenic | 40.0% | |
| GGAATGGATCAAGAGTCAAA+TGG | - | tig0020748:39196-39215 | None:intergenic | 40.0% | |
| ACTAGTAAACGGCCTACAGT+TGG | + | tig0020748:38771-38790 | Msa1385370:CDS | 45.0% | |
| CTAAGTTCGCTTCCAACTGT+AGG | - | tig0020748:38786-38805 | None:intergenic | 45.0% | |
| GGAAAAGCGAAAGGTTCAAC+CGG | + | tig0020748:38924-38943 | Msa1385370:CDS | 45.0% | |
| !! | ATGGATTGGCAATTCTTCGC+GGG | - | tig0020748:39177-39196 | None:intergenic | 45.0% |
| GGCCGTTTACTAGTTAGTCC+TGG | - | tig0020748:38765-38784 | None:intergenic | 50.0% | |
| CGTATCGCTGGAAAAGCGAA+AGG | + | tig0020748:38915-38934 | Msa1385370:CDS | 50.0% | |
| !! | TGGATTGGCAATTCTTCGCG+GGG | - | tig0020748:39176-39195 | None:intergenic | 50.0% |
| GATTGGCAATTCTTCGCGGG+GGG | - | tig0020748:39174-39193 | None:intergenic | 55.0% | |
| ! | GGATTGGCAATTCTTCGCGG+GGG | - | tig0020748:39175-39194 | None:intergenic | 55.0% |
| CGTCGTCTGGGGGCTTTACC+AGG | + | tig0020748:38744-38763 | Msa1385370:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020748 | gene | 38708 | 39313 | 38708 | ID=Msa1385370;Name=Msa1385370 |
| tig0020748 | mRNA | 38708 | 39313 | 38708 | ID=Msa1385370-mRNA-1;Parent=Msa1385370;Name=Msa1385370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201 |
| tig0020748 | exon | 38708 | 39313 | 38708 | ID=Msa1385370-mRNA-1:exon:9548;Parent=Msa1385370-mRNA-1 |
| tig0020748 | CDS | 38708 | 39313 | 38708 | ID=Msa1385370-mRNA-1:cds;Parent=Msa1385370-mRNA-1 |
| Gene Sequence |
| Protein sequence |
