AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1398720


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1398720 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398720 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398720 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398720 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398720 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398720 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1398720 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1398720 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0022279:-79473 Msa1398720:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0022279:+79493 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0022279:-79504 Msa1398720:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0022279:-79804 Msa1398720:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0022279:-79748 Msa1398720:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0022279:-79445 Msa1398720:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0022279:+79533 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0022279:-79947 Msa1398720:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0022279:+79624 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0022279:+79498 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0022279:+79679 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0022279:-79472 Msa1398720:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0022279:-79468 Msa1398720:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0022279:+79924 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0022279:+79519 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0022279:-79783 Msa1398720:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0022279:+79680 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0022279:+79945 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0022279:+79532 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0022279:-79604 Msa1398720:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0022279:+79606 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0022279:+79764 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0022279:-79936 Msa1398720:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0022279:-79603 Msa1398720:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0022279:+79687 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0022279:+79514 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0022279:+79536 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0022279:+79534 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0022279:-80004 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0022279:-80005 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0022279:-79792 Msa1398720:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0022279:+79664 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0022279:+79535 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0022279:79942-79961 Msa1398720:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0022279:79943-79962 Msa1398720:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0022279:79970-79989 Msa1398720:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0022279:79569-79588 Msa1398720:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0022279:79654-79673 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0022279:79689-79708 Msa1398720:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0022279:79920-79939 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0022279:79925-79944 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0022279:79947-79966 Msa1398720:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0022279:79439-79458 Msa1398720:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0022279:79440-79459 Msa1398720:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0022279:79667-79686 Msa1398720:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0022279:79688-79707 Msa1398720:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0022279:79754-79773 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0022279:79752-79771 Msa1398720:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0022279:79812-79831 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0022279:79811-79830 Msa1398720:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0022279:79812-79831 Msa1398720:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0022279:79911-79930 Msa1398720:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0022279:79437-79456 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0022279:79441-79460 Msa1398720:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0022279:79468-79487 Msa1398720:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0022279:79694-79713 Msa1398720:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0022279:79738-79757 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0022279:79739-79758 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0022279:79442-79461 Msa1398720:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0022279:79520-79539 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0022279:79611-79630 Msa1398720:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0022279:79731-79750 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0022279:79794-79813 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0022279:79886-79905 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0022279:79899-79918 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0022279:79904-79923 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0022279:79479-79498 Msa1398720:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0022279:79494-79513 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0022279:79632-79651 Msa1398720:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0022279:79885-79904 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0022279:79473-79492 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0022279:79623-79642 Msa1398720:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0022279:79884-79903 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0022279:79882-79901 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0022279:79883-79902 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0022279:79452-79471 Msa1398720:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0022279 gene 79416 80021 79416 ID=Msa1398720;Name=Msa1398720
tig0022279 mRNA 79416 80021 79416 ID=Msa1398720-mRNA-1;Parent=Msa1398720;Name=Msa1398720-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0022279 exon 79416 80021 79416 ID=Msa1398720-mRNA-1:exon:16776;Parent=Msa1398720-mRNA-1
tig0022279 CDS 79416 80021 79416 ID=Msa1398720-mRNA-1:cds;Parent=Msa1398720-mRNA-1
Gene Sequence

>Msa1398720

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1398720

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*