Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0582520 | YP_009141617.1 | 100.000 | 510 | 0 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1021 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0582520 | sp|Q9BBS3|ATPA_LOTJA | 97.255 | 510 | 14 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1001 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0582520 | A0A1J0I0I3 | 100.000 | 510 | 0 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1021 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000220 | Msa0582520 | 0.800987 | 1.113577e-48 | -8.615850e-47 |
| Msa0002110 | Msa0582520 | 0.803711 | 3.059708e-49 | -8.615850e-47 |
| Msa0002690 | Msa0582520 | 0.846703 | 1.839033e-59 | -8.615850e-47 |
| Msa0002700 | Msa0582520 | 0.845035 | 5.219078e-59 | -8.615850e-47 |
| Msa0003370 | Msa0582520 | 0.817408 | 3.348722e-52 | -8.615850e-47 |
| Msa0004180 | Msa0582520 | 0.803519 | 3.353475e-49 | -8.615850e-47 |
| Msa0016830 | Msa0582520 | 0.828205 | 1.023035e-54 | -8.615850e-47 |
| Msa0018010 | Msa0582520 | 0.810298 | 1.235984e-50 | -8.615850e-47 |
| Msa0021570 | Msa0582520 | 0.844572 | 6.959137e-59 | -8.615850e-47 |
| Msa0028410 | Msa0582520 | 0.835931 | 1.259166e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0582520 | MtrunA17_Chr4g0015651 | 98.821 | 509 | 6 | 0 | 1 | 509 | 1 | 509 | 0.0 | 1004 |
| Msa0582520 | MtrunA17_Chr4g0016741 | 98.821 | 509 | 6 | 0 | 1 | 509 | 1 | 509 | 0.0 | 1004 |
| Msa0582520 | MtrunA17_CPg0492941 | 98.821 | 509 | 6 | 0 | 1 | 509 | 1 | 509 | 0.0 | 1004 |
| Msa0582520 | MtrunA17_MTg0490471 | 61.310 | 504 | 175 | 5 | 3 | 492 | 4 | 501 | 0.0 | 601 |
| Msa0582520 | MtrunA17_Chr2g0278821 | 91.538 | 260 | 22 | 0 | 221 | 480 | 2 | 261 | 4.68e-173 | 489 |
| Msa0582520 | MtrunA17_Chr2g0286451 | 91.803 | 183 | 15 | 0 | 79 | 261 | 7 | 189 | 4.91e-115 | 338 |
| Msa0582520 | MtrunA17_Chr4g0040631 | 96.748 | 123 | 4 | 0 | 1 | 123 | 1 | 123 | 1.09e-74 | 232 |
| Msa0582520 | MtrunA17_Chr4g0061471 | 89.333 | 75 | 8 | 0 | 100 | 174 | 1 | 75 | 1.92e-39 | 137 |
| Msa0582520 | MtrunA17_Chr1g0211291 | 30.363 | 303 | 191 | 5 | 87 | 371 | 84 | 384 | 5.22e-29 | 119 |
| Msa0582520 | MtrunA17_Chr7g0250051 | 30.132 | 302 | 192 | 5 | 87 | 371 | 83 | 382 | 1.82e-28 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0582520 | ATCG00120.1 | 93.294 | 507 | 34 | 0 | 1 | 507 | 1 | 507 | 0.0 | 956 |
| Msa0582520 | AT2G07698.1 | 57.916 | 499 | 196 | 3 | 5 | 492 | 276 | 771 | 0.0 | 577 |
| Msa0582520 | ATMG01190.1 | 57.715 | 499 | 197 | 3 | 5 | 492 | 6 | 501 | 0.0 | 572 |
| Msa0582520 | AT4G38510.1 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0582520 | AT4G38510.3 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0582520 | AT4G38510.4 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0582520 | AT4G38510.2 | 30.464 | 302 | 191 | 5 | 87 | 371 | 83 | 382 | 8.56e-30 | 122 |
| Msa0582520 | AT4G38510.5 | 30.464 | 302 | 191 | 5 | 87 | 371 | 90 | 389 | 8.84e-30 | 122 |
| Msa0582520 | AT1G76030.1 | 30.464 | 302 | 191 | 5 | 87 | 371 | 82 | 381 | 7.41e-29 | 119 |
| Msa0582520 | AT1G20260.1 | 29.703 | 303 | 193 | 5 | 87 | 371 | 82 | 382 | 4.64e-28 | 117 |
Find 119 sgRNAs with CRISPR-Local
Find 260 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCCATGAAACAGGTAGC+TGG | 0.558354 | 4_2:+1059174 | Msa0582520:CDS |
| AAATATTTGGCCATCTGTAA+TGG | 0.434228 | 4_2:-1059064 | None:intergenic |
| AAATGTTGGTGTTGTATTAA+TGG | 0.258988 | 4_2:+1058264 | Msa0582520:CDS |
| AAATTTCAGCTTCGGAATCT+CGG | 0.492871 | 4_2:+1058416 | Msa0582520:CDS |
| AACTACTTTACAAGAAAGAG+GGG | 0.635218 | 4_2:+1058684 | Msa0582520:CDS |
| AAGCTGAAACATCTCCTGAT+TGG | 0.409535 | 4_2:-1059020 | None:intergenic |
| AATCAGACCAGCTATTAATG+TGG | 0.625394 | 4_2:+1059113 | Msa0582520:CDS |
| AATGGAGTACACTATTATAG+TGG | 0.544433 | 4_2:+1058708 | Msa0582520:CDS |
| AATGTAATTTCCATTACAGA+TGG | 0.557336 | 4_2:+1059054 | Msa0582520:CDS |
| AATGTTGGTGTTGTATTAAT+GGG | 0.391664 | 4_2:+1058265 | Msa0582520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATATTGCTCAATACGTTCA+CGG | - | chr4_2:1058096-1058115 | None:intergenic | 30.0% | |
| TGAATTAGTCGAATTTGAAG+AGG | + | chr4_2:1058207-1058226 | Msa0582520:CDS | 30.0% | |
| GAATTAGTCGAATTTGAAGA+GGG | + | chr4_2:1058208-1058227 | Msa0582520:CDS | 30.0% | |
| TTGATTCTATGATCCCTATA+GGG | + | chr4_2:1058515-1058534 | Msa0582520:CDS | 30.0% | |
| AGATACAATTCTCAATCAAC+AGG | + | chr4_2:1058597-1058616 | Msa0582520:CDS | 30.0% | |
| GATACAATTCTCAATCAACA+GGG | + | chr4_2:1058598-1058617 | Msa0582520:CDS | 30.0% | |
| GTAACTACTTTACAAGAAAG+AGG | + | chr4_2:1058682-1058701 | Msa0582520:CDS | 30.0% | |
| AACTACTTTACAAGAAAGAG+GGG | + | chr4_2:1058684-1058703 | Msa0582520:CDS | 30.0% | |
| AATGGAGTACACTATTATAG+TGG | + | chr4_2:1058708-1058727 | Msa0582520:CDS | 30.0% | |
| TTCGTAATAGAAGAGACATT+TGG | - | chr4_2:1058873-1058892 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 1058055 | 1059587 | 1058055 | ID=Msa0582520;Name=Msa0582520 |
| chr4_2 | mRNA | 1058055 | 1059587 | 1058055 | ID=Msa0582520-mRNA-1;Parent=Msa0582520;Name=Msa0582520-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|510 |
| chr4_2 | exon | 1058055 | 1059587 | 1058055 | ID=Msa0582520-mRNA-1:exon:196;Parent=Msa0582520-mRNA-1 |
| chr4_2 | CDS | 1058055 | 1059587 | 1058055 | ID=Msa0582520-mRNA-1:cds;Parent=Msa0582520-mRNA-1 |
| Gene Sequence |
>Msa0582520 ATGGTAACCATTCGTGCAGATGAAATTAGTAAAATTATCCGTGAACGTATTGAGCAATATAATACCGAAGTAAAAATTGTAAATACAGGTACTGTACTTCAAGTAGGCGACGGCATTGCTCGTATTTATGGTCTTGATGAAGTAATGGCAGGTGAATTAGTCGAATTTGAAGAGGGTACTGTAGGCATTGCTTTGAATTTGGAATCCAAAAATGTTGGTGTTGTATTAATGGGTGATGGTTTGCTGATACAAGAGGGAAGTTCGGTAAAAGCAACAGGAAGAATTGCTCAGATACCAGTAAGTGAGGGTTATTTGGGTCGTGTTGTAAATGCCCTAGCTAAACCTATTGATGGTCGAGGAGAAATTTCAGCTTCGGAATCTCGGTTAATCGAATCTCCCGCTCCCGGTATTATTTCCAGACGTTCCGTGTATGAGCCTCTTCAAACGGGACTTATTGCTATTGATTCTATGATCCCTATAGGGCGTGGCCAGCGAGAATTAATTATTGGGGACAGACAAACAGGTAAAACAGCAGTAGCAACAGATACAATTCTCAATCAACAGGGACAAAATGTAATATGTGTTTATGTAGCTATTGGTCAAAAAGCATCTTCTGTGGCTCAAGTGGTAACTACTTTACAAGAAAGAGGGGCAATGGAGTACACTATTATAGTGGCTGAAACAGCAAATTCTCCAGCTACATTACAATACCTCGCTCCTTATACAGGTGCAGCTCTGGCTGAATATTTTATGTACCGTGAACGTCACACTTTAATCATTTATGACGATCCCTCCAAACAAGCACAGGCTTATCGCCAAATGTCTCTTCTATTACGAAGACCACCAGGTCGCGAAGCGTATCCCGGAGATGTTTTTTATTTACATTCTCGTCTTTTGGAAAGAGCCGCTAAATTAAGTTCTCAGTTAGGTGAAGGAAGTATGACTGCTTTACCAATAGTTGAGACCCAATCAGGAGATGTTTCAGCTTATATTCCTACTAATGTAATTTCCATTACAGATGGCCAAATATTTTTATCTGCCGATCTATTCAATGCTGGAATCAGACCAGCTATTAATGTGGGTATTTCCGTTTCCAGAGTTGGATCGGCGGCTCAAATTAAAGCCATGAAACAGGTAGCTGGTAAATTAAAATTGGAATTGGCACAATTCGCAGAATTAGAAGCTTTTGCACAATTCGCTTCTGATCTCGATAAAGCTACGCAAAATCAATTGGCAAGAGGTCAACGATTGCGCGAGTTGCTTAAACAATCCCAATCAGCTCCTCTCACTGTGGAAGAACAGATAATAACTATTTATACTGGAACGAATGGTTATCTTGATTCATTAGAAATTGATCAGGTACGTAAATTTCTCGTTGAGTTAAGGGCTTATTTAAAAACGAATAAACCTAAATTTAACGAAATCATATCTTCTACCAAGACATTCACTGGGGAAGCAGAAGCCCTTTTGAAGGAAGCTATTCAGGAACAGATGGAACTCTTTTTACTTCAGGAACAAGTAGAAAAAAATTGA |
| Protein sequence |
>Msa0582520 MVTIRADEISKIIRERIEQYNTEVKIVNTGTVLQVGDGIARIYGLDEVMAGELVEFEEGTVGIALNLESKNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEGYLGRVVNALAKPIDGRGEISASESRLIESPAPGIISRRSVYEPLQTGLIAIDSMIPIGRGQRELIIGDRQTGKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTTLQERGAMEYTIIVAETANSPATLQYLAPYTGAALAEYFMYRERHTLIIYDDPSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSSQLGEGSMTALPIVETQSGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVGSAAQIKAMKQVAGKLKLELAQFAELEAFAQFASDLDKATQNQLARGQRLRELLKQSQSAPLTVEEQIITIYTGTNGYLDSLEIDQVRKFLVELRAYLKTNKPKFNEIISSTKTFTGEAEALLKEAIQEQMELFLLQEQVEKN* |
