AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354950 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354950 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354950 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354950 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354950 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354950 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1354950 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354950 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0014543:-619162 Msa1354950:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0014543:+619182 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0014543:-619193 Msa1354950:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0014543:-619493 Msa1354950:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0014543:-619437 Msa1354950:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0014543:-619134 Msa1354950:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0014543:+619222 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0014543:-619636 Msa1354950:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0014543:+619313 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0014543:+619187 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0014543:+619368 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0014543:-619161 Msa1354950:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0014543:-619157 Msa1354950:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0014543:+619613 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0014543:+619208 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0014543:-619472 Msa1354950:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0014543:+619369 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0014543:+619634 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0014543:+619221 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0014543:-619293 Msa1354950:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0014543:+619295 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0014543:+619453 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0014543:-619625 Msa1354950:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0014543:-619292 Msa1354950:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0014543:+619376 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0014543:+619203 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0014543:+619225 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0014543:+619223 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0014543:-619693 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0014543:-619694 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0014543:-619481 Msa1354950:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0014543:+619353 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0014543:+619224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0014543:619631-619650 Msa1354950:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0014543:619632-619651 Msa1354950:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0014543:619659-619678 Msa1354950:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0014543:619258-619277 Msa1354950:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0014543:619343-619362 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0014543:619378-619397 Msa1354950:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0014543:619609-619628 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0014543:619614-619633 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0014543:619636-619655 Msa1354950:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0014543:619128-619147 Msa1354950:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0014543:619129-619148 Msa1354950:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0014543:619356-619375 Msa1354950:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0014543:619377-619396 Msa1354950:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0014543:619443-619462 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0014543:619441-619460 Msa1354950:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0014543:619501-619520 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0014543:619500-619519 Msa1354950:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0014543:619501-619520 Msa1354950:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0014543:619600-619619 Msa1354950:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0014543:619126-619145 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0014543:619130-619149 Msa1354950:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0014543:619157-619176 Msa1354950:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0014543:619383-619402 Msa1354950:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0014543:619427-619446 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0014543:619428-619447 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0014543:619131-619150 Msa1354950:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0014543:619209-619228 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0014543:619300-619319 Msa1354950:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0014543:619420-619439 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0014543:619483-619502 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0014543:619575-619594 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0014543:619588-619607 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0014543:619593-619612 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0014543:619168-619187 Msa1354950:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0014543:619183-619202 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0014543:619321-619340 Msa1354950:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0014543:619574-619593 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0014543:619162-619181 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0014543:619312-619331 Msa1354950:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0014543:619573-619592 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0014543:619571-619590 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0014543:619572-619591 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0014543:619141-619160 Msa1354950:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 619105 619710 619105 ID=Msa1354950;Name=Msa1354950
tig0014543 mRNA 619105 619710 619105 ID=Msa1354950-mRNA-1;Parent=Msa1354950;Name=Msa1354950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0014543 exon 619105 619710 619105 ID=Msa1354950-mRNA-1:exon:9276;Parent=Msa1354950-mRNA-1
tig0014543 CDS 619105 619710 619105 ID=Msa1354950-mRNA-1:cds;Parent=Msa1354950-mRNA-1
Gene Sequence

>Msa1354950

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1354950

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*