Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867640 | YP_009141600.1 | 100.000 | 201 | 0 | 0 | 1 | 201 | 1 | 201 | 3.55e-143 | 407 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867640 | sp|Q9BBT5|RR4_LOTJA | 95.522 | 201 | 9 | 0 | 1 | 201 | 1 | 201 | 8.95e-140 | 391 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867640 | A0A0F6NLD0 | 100.000 | 201 | 0 | 0 | 1 | 201 | 1 | 201 | 1.70e-143 | 407 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0095170 | Msa0867640 | 0.823413 | 1.404212e-53 | -8.615850e-47 |
| Msa0095190 | Msa0867640 | 0.805386 | 1.368831e-49 | -8.615850e-47 |
| Msa0095210 | Msa0867640 | 0.824499 | 7.809232e-54 | -8.615850e-47 |
| Msa0095290 | Msa0867640 | 0.816560 | 5.193055e-52 | -8.615850e-47 |
| Msa0173880 | Msa0867640 | 0.832641 | 8.419594e-56 | -8.615850e-47 |
| Msa0202640 | Msa0867640 | 0.833536 | 5.040152e-56 | -8.615850e-47 |
| Msa0221840 | Msa0867640 | 0.809328 | 1.997887e-50 | -8.615850e-47 |
| Msa0370210 | Msa0867640 | 0.817297 | 3.548371e-52 | -8.615850e-47 |
| Msa0398630 | Msa0867640 | 0.821031 | 5.010864e-53 | -8.615850e-47 |
| Msa0449920 | Msa0867640 | 0.805959 | 1.037517e-49 | -8.615850e-47 |
| Msa0268170 | Msa0867640 | 0.834354 | 3.146253e-56 | -8.615850e-47 |
| Msa0268190 | Msa0867640 | 0.818072 | 2.371697e-52 | -8.615850e-47 |
| Msa0268370 | Msa0867640 | 0.810594 | 1.066701e-50 | -8.615850e-47 |
| Msa0268390 | Msa0867640 | 0.836345 | 9.878824e-57 | -8.615850e-47 |
| Msa0268400 | Msa0867640 | 0.824253 | 8.919831e-54 | -8.615850e-47 |
| Msa0268410 | Msa0867640 | 0.837652 | 4.580644e-57 | -8.615850e-47 |
| Msa0268430 | Msa0867640 | 0.818181 | 2.241424e-52 | -8.615850e-47 |
| Msa0268530 | Msa0867640 | 0.818841 | 1.587748e-52 | -8.615850e-47 |
| Msa0268550 | Msa0867640 | 0.839682 | 1.369397e-57 | -8.615850e-47 |
| Msa0268560 | Msa0867640 | 0.808707 | 2.713939e-50 | -8.615850e-47 |
| Msa0268570 | Msa0867640 | 0.807667 | 4.519267e-50 | -8.615850e-47 |
| Msa0268580 | Msa0867640 | 0.824108 | 9.647966e-54 | -8.615850e-47 |
| Msa0268630 | Msa0867640 | 0.821700 | 3.512831e-53 | -8.615850e-47 |
| Msa0268640 | Msa0867640 | 0.820130 | 8.071479e-53 | -8.615850e-47 |
| Msa0268720 | Msa0867640 | 0.834332 | 3.185795e-56 | -8.615850e-47 |
| Msa0268740 | Msa0867640 | 0.823492 | 1.345517e-53 | -8.615850e-47 |
| Msa0268750 | Msa0867640 | 0.805770 | 1.136770e-49 | -8.615850e-47 |
| Msa0268760 | Msa0867640 | 0.807288 | 5.438885e-50 | -8.615850e-47 |
| Msa0268830 | Msa0867640 | 0.825099 | 5.637407e-54 | -8.615850e-47 |
| Msa0268840 | Msa0867640 | 0.828931 | 6.832218e-55 | -8.615850e-47 |
| Msa0268860 | Msa0867640 | 0.817745 | 2.811354e-52 | -8.615850e-47 |
| Msa0268920 | Msa0867640 | 0.815819 | 7.602597e-52 | -8.615850e-47 |
| Msa0268940 | Msa0867640 | 0.828482 | 8.771758e-55 | -8.615850e-47 |
| Msa0268950 | Msa0867640 | 0.811926 | 5.482012e-51 | -8.615850e-47 |
| Msa0268960 | Msa0867640 | 0.806252 | 9.002812e-50 | -8.615850e-47 |
| Msa0269040 | Msa0867640 | 0.814694 | 1.352197e-51 | -8.615850e-47 |
| Msa0269050 | Msa0867640 | 0.839860 | 1.230482e-57 | -8.615850e-47 |
| Msa0269070 | Msa0867640 | 0.815201 | 1.043649e-51 | -8.615850e-47 |
| Msa0344180 | Msa0867640 | 0.820432 | 6.880183e-53 | -8.615850e-47 |
| Msa0344190 | Msa0867640 | 0.829275 | 5.637506e-55 | -8.615850e-47 |
| Msa0344210 | Msa0867640 | 0.808872 | 2.502248e-50 | -8.615850e-47 |
| Msa0344280 | Msa0867640 | 0.821473 | 3.962417e-53 | -8.615850e-47 |
| Msa0344300 | Msa0867640 | 0.843533 | 1.321813e-58 | -8.615850e-47 |
| Msa0344310 | Msa0867640 | 0.801913 | 7.194967e-49 | -8.615850e-47 |
| Msa0344330 | Msa0867640 | 0.812615 | 3.878975e-51 | -8.615850e-47 |
| Msa0483990 | Msa0867640 | 0.813979 | 1.945738e-51 | -8.615850e-47 |
| Msa0571220 | Msa0867640 | 0.801326 | 9.493046e-49 | -8.615850e-47 |
| Msa0582510 | Msa0867640 | 0.828713 | 7.714612e-55 | -8.615850e-47 |
| Msa0582520 | Msa0867640 | 0.836361 | 9.791609e-57 | -8.615850e-47 |
| Msa0582540 | Msa0867640 | 0.812430 | 4.255736e-51 | -8.615850e-47 |
| Msa0582610 | Msa0867640 | 0.809128 | 2.205368e-50 | -8.615850e-47 |
| Msa0582630 | Msa0867640 | 0.814861 | 1.241817e-51 | -8.615850e-47 |
| Msa0582640 | Msa0867640 | 0.803056 | 4.182335e-49 | -8.615850e-47 |
| Msa0582660 | Msa0867640 | 0.831744 | 1.403341e-55 | -8.615850e-47 |
| Msa0582690 | Msa0867640 | 0.818952 | 1.498122e-52 | -8.615850e-47 |
| Msa0582700 | Msa0867640 | 0.846387 | 2.242874e-59 | -8.615850e-47 |
| Msa0582720 | Msa0867640 | 0.825237 | 5.230098e-54 | -8.615850e-47 |
| Msa0582730 | Msa0867640 | 0.805338 | 1.400757e-49 | -8.615850e-47 |
| Msa0582750 | Msa0867640 | 0.826780 | 2.248607e-54 | -8.615850e-47 |
| Msa0582760 | Msa0867640 | 0.811048 | 8.507299e-51 | -8.615850e-47 |
| Msa0582780 | Msa0867640 | 0.821437 | 4.040224e-53 | -8.615850e-47 |
| Msa0582810 | Msa0867640 | 0.818239 | 2.174970e-52 | -8.615850e-47 |
| Msa0582820 | Msa0867640 | 0.841329 | 5.075517e-58 | -8.615850e-47 |
| Msa0582840 | Msa0867640 | 0.819159 | 1.344392e-52 | -8.615850e-47 |
| Msa0582890 | Msa0867640 | 0.842777 | 2.102337e-58 | -8.615850e-47 |
| Msa0582910 | Msa0867640 | 0.824669 | 7.121122e-54 | -8.615850e-47 |
| Msa0582920 | Msa0867640 | 0.810984 | 8.783602e-51 | -8.615850e-47 |
| Msa0582930 | Msa0867640 | 0.815917 | 7.228679e-52 | -8.615850e-47 |
| Msa0591370 | Msa0867640 | 0.806466 | 8.115074e-50 | -8.615850e-47 |
| Msa0867640 | Msa0867650 | 0.836613 | 8.445519e-57 | -8.615850e-47 |
| Msa0867640 | Msa0924850 | 0.805244 | 1.465788e-49 | -8.615850e-47 |
| Msa0867640 | Msa0924870 | 0.820583 | 6.353146e-53 | -8.615850e-47 |
| Msa0867640 | Msa0924880 | 0.824701 | 6.998348e-54 | -8.615850e-47 |
| Msa0867640 | Msa0924990 | 0.822935 | 1.814763e-53 | -8.615850e-47 |
| Msa0867640 | Msa0925040 | 0.819464 | 1.145362e-52 | -8.615850e-47 |
| Msa0867640 | Msa0931070 | 0.829533 | 4.880008e-55 | -8.615850e-47 |
| Msa0867640 | Msa0931080 | 0.828981 | 6.643207e-55 | -8.615850e-47 |
| Msa0867640 | Msa0931100 | 0.829122 | 6.140359e-55 | -8.615850e-47 |
| Msa0867640 | Msa0931130 | 0.826397 | 2.774362e-54 | -8.615850e-47 |
| Msa0867640 | Msa0931140 | 0.830299 | 3.176519e-55 | -8.615850e-47 |
| Msa0867640 | Msa0931160 | 0.806379 | 8.465040e-50 | -8.615850e-47 |
| Msa0867640 | Msa0931190 | 0.848413 | 6.226154e-60 | -8.615850e-47 |
| Msa0867640 | Msa0931210 | 0.839895 | 1.204877e-57 | -8.615850e-47 |
| Msa0867640 | Msa0931220 | 0.805036 | 1.620647e-49 | -8.615850e-47 |
| Msa0867640 | Msa0931230 | 0.808276 | 3.352761e-50 | -8.615850e-47 |
| Msa0867640 | Msa0931240 | 0.825366 | 4.874573e-54 | -8.615850e-47 |
| Msa0867640 | Msa0931260 | 0.821760 | 3.401745e-53 | -8.615850e-47 |
| Msa0867640 | Msa0931270 | 0.817529 | 3.146163e-52 | -8.615850e-47 |
| Msa0867640 | Msa0983700 | 0.832570 | 8.768956e-56 | -8.615850e-47 |
| Msa0867640 | Msa0983720 | 0.826869 | 2.140667e-54 | -8.615850e-47 |
| Msa0867640 | Msa1086050 | 0.824071 | 9.845179e-54 | -8.615850e-47 |
| Msa0867640 | Msa1086070 | 0.822362 | 2.466578e-53 | -8.615850e-47 |
| Msa0867640 | Msa1086080 | 0.803795 | 2.940084e-49 | -8.615850e-47 |
| Msa0867640 | Msa1086100 | 0.824321 | 8.598639e-54 | -8.615850e-47 |
| Msa0867640 | Msa1086140 | 0.826083 | 3.296221e-54 | -8.615850e-47 |
| Msa0867640 | Msa1086150 | 0.832260 | 1.046165e-55 | -8.615850e-47 |
| Msa0867640 | Msa1086180 | 0.823211 | 1.565242e-53 | -8.615850e-47 |
| Msa0867640 | Msa1086190 | 0.808911 | 2.454520e-50 | -8.615850e-47 |
| Msa0867640 | Msa1086200 | 0.802638 | 5.102905e-49 | -8.615850e-47 |
| Msa0867640 | Msa1086210 | 0.814385 | 1.582867e-51 | -8.615850e-47 |
| Msa0867640 | Msa1086270 | 0.827242 | 1.743080e-54 | -8.615850e-47 |
| Msa0867640 | Msa1086280 | 0.838471 | 2.819580e-57 | -8.615850e-47 |
| Msa0867640 | Msa1086300 | 0.807882 | 4.067785e-50 | -8.615850e-47 |
| Msa0867640 | Msa1086360 | 0.817537 | 3.131940e-52 | -8.615850e-47 |
| Msa0867640 | Msa1086380 | 0.821157 | 4.688344e-53 | -8.615850e-47 |
| Msa0867640 | Msa1086430 | 0.812323 | 4.492345e-51 | -8.615850e-47 |
| Msa0867640 | Msa1086450 | 0.830899 | 2.264295e-55 | -8.615850e-47 |
| Msa0867640 | Msa1086470 | 0.834323 | 3.203194e-56 | -8.615850e-47 |
| Msa0867640 | Msa1086660 | 0.828879 | 7.032791e-55 | -8.615850e-47 |
| Msa0867640 | Msa1086860 | 0.824115 | 9.615397e-54 | -8.615850e-47 |
| Msa0867640 | Msa1086880 | 0.821877 | 3.196441e-53 | -8.615850e-47 |
| Msa0867640 | Msa1086890 | 0.804439 | 2.158985e-49 | -8.615850e-47 |
| Msa0867640 | Msa1086910 | 0.816321 | 5.874905e-52 | -8.615850e-47 |
| Msa0867640 | Msa1091700 | 0.803127 | 4.042503e-49 | -8.615850e-47 |
| Msa0867640 | Msa1143120 | 0.824304 | 8.677824e-54 | -8.615850e-47 |
| Msa0867640 | Msa1143130 | 0.818252 | 2.159890e-52 | -8.615850e-47 |
| Msa0867640 | Msa1143160 | 0.821836 | 3.267131e-53 | -8.615850e-47 |
| Msa0867640 | Msa1143180 | 0.823189 | 1.583377e-53 | -8.615850e-47 |
| Msa0867640 | Msa1269400 | 0.856537 | 3.008046e-62 | -8.615850e-47 |
| Msa0867640 | Msa1269420 | 0.878603 | 2.456779e-69 | -8.615850e-47 |
| Msa0867640 | Msa1305090 | 0.810152 | 1.328653e-50 | -8.615850e-47 |
| Msa0867640 | Msa1354320 | 0.816678 | 4.886753e-52 | -8.615850e-47 |
| Msa0867640 | Msa1354330 | 0.807139 | 5.847496e-50 | -8.615850e-47 |
| Msa0867640 | Msa1354340 | 0.819563 | 1.087793e-52 | -8.615850e-47 |
| Msa0867640 | Msa1354350 | 0.876587 | 1.239805e-68 | -8.615850e-47 |
| Msa0867640 | Msa1354380 | 0.882955 | 6.743737e-71 | -8.615850e-47 |
| Msa0867640 | Msa1354390 | 0.868780 | 5.063611e-66 | -8.615850e-47 |
| Msa0867640 | Msa1354880 | 0.824598 | 7.401094e-54 | -8.615850e-47 |
| Msa0867640 | Msa1354900 | 0.838182 | 3.347397e-57 | -8.615850e-47 |
| Msa0867640 | Msa1354910 | 0.810923 | 9.052735e-51 | -8.615850e-47 |
| Msa0867640 | Msa1354920 | 0.805092 | 1.577448e-49 | -8.615850e-47 |
| Msa0867640 | Msa1354930 | 0.827097 | 1.888201e-54 | -8.615850e-47 |
| Msa0867640 | Msa1354950 | 0.858347 | 8.767427e-63 | -8.615850e-47 |
| Msa0867640 | Msa1355050 | 0.819132 | 1.363416e-52 | -8.615850e-47 |
| Msa0867640 | Msa1355070 | 0.828136 | 1.063239e-54 | -8.615850e-47 |
| Msa0867640 | Msa1355080 | 0.813449 | 2.545901e-51 | -8.615850e-47 |
| Msa0867640 | Msa1355090 | 0.802240 | 6.160875e-49 | -8.615850e-47 |
| Msa0867640 | Msa1355100 | 0.817259 | 3.618593e-52 | -8.615850e-47 |
| Msa0867640 | Msa1366000 | 0.867439 | 1.367113e-65 | -8.615850e-47 |
| Msa0867640 | Msa1366010 | 0.864682 | 1.020041e-64 | -8.615850e-47 |
| Msa0867640 | Msa1366040 | 0.826400 | 2.770238e-54 | -8.615850e-47 |
| Msa0867640 | Msa1366050 | 0.882727 | 8.170426e-71 | -8.615850e-47 |
| Msa0867640 | Msa1368270 | 0.875192 | 3.739555e-68 | -8.615850e-47 |
| Msa0867640 | Msa1368280 | 0.838429 | 2.890670e-57 | -8.615850e-47 |
| Msa0867640 | Msa1368290 | 0.872522 | 2.982573e-67 | -8.615850e-47 |
| Msa0867640 | Msa1368300 | 0.852911 | 3.382071e-61 | -8.615850e-47 |
| Msa0867640 | Msa1368310 | 0.800808 | 1.211347e-48 | -8.615850e-47 |
| Msa0867640 | Msa1380570 | 0.815735 | 7.939118e-52 | -8.615850e-47 |
| Msa0867640 | Msa1380590 | 0.833177 | 6.192825e-56 | -8.615850e-47 |
| Msa0867640 | Msa1380600 | 0.836988 | 6.774744e-57 | -8.615850e-47 |
| Msa0867640 | Msa1380610 | 0.812976 | 3.233109e-51 | -8.615850e-47 |
| Msa0867640 | Msa1380620 | 0.819206 | 1.311520e-52 | -8.615850e-47 |
| Msa0867640 | Msa1380640 | 0.880478 | 5.312877e-70 | -8.615850e-47 |
| Msa0867640 | Msa1380650 | 0.814687 | 1.357287e-51 | -8.615850e-47 |
| Msa0867640 | Msa1380710 | 0.840713 | 7.368348e-58 | -8.615850e-47 |
| Msa0867640 | Msa1385370 | 0.838212 | 3.287658e-57 | -8.615850e-47 |
| Msa0867640 | Msa1394030 | 0.813641 | 2.309130e-51 | -8.615850e-47 |
| Msa0867640 | Msa1394040 | 0.821209 | 4.559184e-53 | -8.615850e-47 |
| Msa0867640 | Msa1394060 | 0.830959 | 2.188383e-55 | -8.615850e-47 |
| Msa0867640 | Msa1394130 | 0.822840 | 1.909872e-53 | -8.615850e-47 |
| Msa0867640 | Msa1394150 | 0.815607 | 8.479558e-52 | -8.615850e-47 |
| Msa0867640 | Msa1394160 | 0.817840 | 2.676091e-52 | -8.615850e-47 |
| Msa0867640 | Msa1394170 | 0.808764 | 2.638842e-50 | -8.615850e-47 |
| Msa0867640 | Msa1394210 | 0.810904 | 9.139592e-51 | -8.615850e-47 |
| Msa0867640 | Msa1394220 | 0.827472 | 1.535501e-54 | -8.615850e-47 |
| Msa0867640 | Msa1394230 | 0.830155 | 3.443915e-55 | -8.615850e-47 |
| Msa0867640 | Msa1394250 | 0.826118 | 3.232913e-54 | -8.615850e-47 |
| Msa0867640 | Msa1398700 | 0.831355 | 1.749337e-55 | -8.615850e-47 |
| Msa0867640 | Msa1398710 | 0.807679 | 4.492260e-50 | -8.615850e-47 |
| Msa0867640 | Msa1398720 | 0.851610 | 7.936381e-61 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867640 | MtrunA17_Chr7g0229301 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867640 | MtrunA17_Chr6g0454441 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867640 | MtrunA17_Chr4g0016451 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867640 | MtrunA17_Chr6g0466281 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867640 | MtrunA17_CPg0493311 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867640 | MtrunA17_Chr7g0229661 | 84.080 | 201 | 13 | 1 | 1 | 201 | 1 | 182 | 1.89e-115 | 327 |
| Msa0867640 | MtrunA17_Chr4g0015291 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 3.32e-108 | 307 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867640 | ATCG00380.1 | 88.060 | 201 | 24 | 0 | 1 | 201 | 1 | 201 | 2.12e-126 | 355 |
Find 33 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAATAATAGATAGTAAA+TGG | 0.189787 | 6_1:+706829 | Msa0867640:CDS |
| GATTGACTAATCCTTTATAT+TGG | 0.284280 | 6_1:-706809 | None:intergenic |
| CTTGATCCATTCCAATATAA+AGG | 0.291752 | 6_1:+706798 | Msa0867640:CDS |
| CTTAAATACGTTCGTATCGC+TGG | 0.342652 | 6_1:+706498 | Msa0867640:CDS |
| ACAATTACTTGAAATGCGTT+TGG | 0.359489 | 6_1:+706554 | Msa0867640:CDS |
| TTTGAAAATTAATGAATTGC+TGG | 0.365999 | 6_1:+706857 | Msa0867640:CDS |
| ATGGATTGGCAATTCTTCGC+GGG | 0.450528 | 6_1:-706769 | None:intergenic |
| TACCAGGACTAACTAGTAAA+CGG | 0.463029 | 6_1:+706355 | Msa0867640:CDS |
| TGAGGTTTGCAACGATAACT+TGG | 0.468099 | 6_1:-706678 | None:intergenic |
| ACTAATCCTTTATATTGGAA+TGG | 0.475526 | 6_1:-706804 | None:intergenic |
| GTCTGTGGTTAACTAATTGA+CGG | 0.477609 | 6_1:-706623 | None:intergenic |
| TCAAATAATAGATAGTAAAT+GGG | 0.487910 | 6_1:+706830 | Msa0867640:CDS |
| ATAATAGATAGTAAATGGGT+TGG | 0.504615 | 6_1:+706834 | Msa0867640:CDS |
| CTAAGTTCGCTTCCAACTGT+AGG | 0.508663 | 6_1:-706378 | None:intergenic |
| GGATCAAGAGTCAAATGGAT+TGG | 0.511159 | 6_1:-706783 | None:intergenic |
| GGAAAAGCGAAAGGTTCAAC+CGG | 0.516182 | 6_1:+706519 | Msa0867640:CDS |
| TCTGTGGTTAACTAATTGAC+GGG | 0.521261 | 6_1:-706622 | None:intergenic |
| GGCCGTTTACTAGTTAGTCC+TGG | 0.527000 | 6_1:-706357 | None:intergenic |
| AATGGATTGGCAATTCTTCG+CGG | 0.545268 | 6_1:-706770 | None:intergenic |
| TCAAGATATTATTACAGCGA+AGG | 0.559737 | 6_1:+706698 | Msa0867640:CDS |
| TTCGCTGTAATAATATCTTG+AGG | 0.560954 | 6_1:-706696 | None:intergenic |
| TAATTGTAGTAAAACTTGAC+CGG | 0.568250 | 6_1:-706538 | None:intergenic |
| ACTAGTAAACGGCCTACAGT+TGG | 0.568372 | 6_1:+706366 | Msa0867640:CDS |
| CAAGATATTATTACAGCGAA+GGG | 0.569002 | 6_1:+706699 | Msa0867640:CDS |
| TTAACTAATTGACGGGCTTG+AGG | 0.581554 | 6_1:-706615 | None:intergenic |
| GGAATGGATCAAGAGTCAAA+TGG | 0.582974 | 6_1:-706788 | None:intergenic |
| GATTGGCAATTCTTCGCGGG+GGG | 0.584547 | 6_1:-706766 | None:intergenic |
| TGGATTGGCAATTCTTCGCG+GGG | 0.610754 | 6_1:-706768 | None:intergenic |
| TGTTTATGTCACGTTACAGA+GGG | 0.632465 | 6_1:+706298 | None:intergenic |
| GTGTTTATGTCACGTTACAG+AGG | 0.633128 | 6_1:+706297 | None:intergenic |
| CGTATCGCTGGAAAAGCGAA+AGG | 0.633501 | 6_1:+706510 | Msa0867640:CDS |
| CATTAACTAAAACATGTCTG+TGG | 0.663264 | 6_1:-706638 | None:intergenic |
| GGATTGGCAATTCTTCGCGG+GGG | 0.681198 | 6_1:-706767 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAATAATAGATAGTAAA+TGG | + | chr6_1:706829-706848 | Msa0867640:CDS | 15.0% |
| !! | TCAAATAATAGATAGTAAAT+GGG | + | chr6_1:706830-706849 | Msa0867640:CDS | 15.0% |
| !! | TTTGAAAATTAATGAATTGC+TGG | + | chr6_1:706857-706876 | Msa0867640:CDS | 20.0% |
| !! | CAAAAATTGCGTTTTCATTA+TGG | + | chr6_1:706456-706475 | Msa0867640:CDS | 25.0% |
| ! | TAATTGTAGTAAAACTTGAC+CGG | - | chr6_1:706541-706560 | None:intergenic | 25.0% |
| !!! | GATAACATACTTTTTCGATT+GGG | + | chr6_1:706576-706595 | Msa0867640:CDS | 25.0% |
| ! | ACTAATCCTTTATATTGGAA+TGG | - | chr6_1:706807-706826 | None:intergenic | 25.0% |
| ! | GATTGACTAATCCTTTATAT+TGG | - | chr6_1:706812-706831 | None:intergenic | 25.0% |
| ! | ATAATAGATAGTAAATGGGT+TGG | + | chr6_1:706834-706853 | Msa0867640:CDS | 25.0% |
| TTTCAAAAAAATACGTCGTC+TGG | + | chr6_1:706326-706345 | Msa0867640:CDS | 30.0% | |
| TTCAAAAAAATACGTCGTCT+GGG | + | chr6_1:706327-706346 | Msa0867640:CDS | 30.0% | |
| ACAATTACTTGAAATGCGTT+TGG | + | chr6_1:706554-706573 | Msa0867640:CDS | 30.0% | |
| !!! | GGATAACATACTTTTTCGAT+TGG | + | chr6_1:706575-706594 | Msa0867640:CDS | 30.0% |
| CATTAACTAAAACATGTCTG+TGG | - | chr6_1:706641-706660 | None:intergenic | 30.0% | |
| !!! | CACAGACATGTTTTAGTTAA+TGG | + | chr6_1:706639-706658 | Msa0867640:CDS | 30.0% |
| TTCGCTGTAATAATATCTTG+AGG | - | chr6_1:706699-706718 | None:intergenic | 30.0% | |
| TCAAGATATTATTACAGCGA+AGG | + | chr6_1:706698-706717 | Msa0867640:CDS | 30.0% | |
| CAAGATATTATTACAGCGAA+GGG | + | chr6_1:706699-706718 | Msa0867640:CDS | 30.0% | |
| CTTGATCCATTCCAATATAA+AGG | + | chr6_1:706798-706817 | Msa0867640:CDS | 30.0% | |
| !!! | CGACGTATTTTTTTGAAACG+AGG | - | chr6_1:706324-706343 | None:intergenic | 35.0% |
| TCAAAAAAATACGTCGTCTG+GGG | + | chr6_1:706328-706347 | Msa0867640:CDS | 35.0% | |
| TACCAGGACTAACTAGTAAA+CGG | + | chr6_1:706355-706374 | Msa0867640:CDS | 35.0% | |
| !!! | CATACTTTTTCGATTGGGTA+TGG | + | chr6_1:706581-706600 | Msa0867640:CDS | 35.0% |
| TCTGTGGTTAACTAATTGAC+GGG | - | chr6_1:706625-706644 | None:intergenic | 35.0% | |
| GTCTGTGGTTAACTAATTGA+CGG | - | chr6_1:706626-706645 | None:intergenic | 35.0% | |
| CAAAAAAATACGTCGTCTGG+GGG | + | chr6_1:706329-706348 | Msa0867640:CDS | 40.0% | |
| !!! | ATTTTTTACTCGAGCGCGAT+TGG | - | chr6_1:706407-706426 | None:intergenic | 40.0% |
| CTTAAATACGTTCGTATCGC+TGG | + | chr6_1:706498-706517 | Msa0867640:CDS | 40.0% | |
| !! | TTAACTAATTGACGGGCTTG+AGG | - | chr6_1:706618-706637 | None:intergenic | 40.0% |
| TGAGGTTTGCAACGATAACT+TGG | - | chr6_1:706681-706700 | None:intergenic | 40.0% | |
| !! | AATGGATTGGCAATTCTTCG+CGG | - | chr6_1:706773-706792 | None:intergenic | 40.0% |
| GGATCAAGAGTCAAATGGAT+TGG | - | chr6_1:706786-706805 | None:intergenic | 40.0% | |
| GGAATGGATCAAGAGTCAAA+TGG | - | chr6_1:706791-706810 | None:intergenic | 40.0% | |
| ACTAGTAAACGGCCTACAGT+TGG | + | chr6_1:706366-706385 | Msa0867640:CDS | 45.0% | |
| CTAAGTTCGCTTCCAACTGT+AGG | - | chr6_1:706381-706400 | None:intergenic | 45.0% | |
| GGAAAAGCGAAAGGTTCAAC+CGG | + | chr6_1:706519-706538 | Msa0867640:CDS | 45.0% | |
| !! | ATGGATTGGCAATTCTTCGC+GGG | - | chr6_1:706772-706791 | None:intergenic | 45.0% |
| GGCCGTTTACTAGTTAGTCC+TGG | - | chr6_1:706360-706379 | None:intergenic | 50.0% | |
| CGTATCGCTGGAAAAGCGAA+AGG | + | chr6_1:706510-706529 | Msa0867640:CDS | 50.0% | |
| !! | TGGATTGGCAATTCTTCGCG+GGG | - | chr6_1:706771-706790 | None:intergenic | 50.0% |
| GATTGGCAATTCTTCGCGGG+GGG | - | chr6_1:706769-706788 | None:intergenic | 55.0% | |
| ! | GGATTGGCAATTCTTCGCGG+GGG | - | chr6_1:706770-706789 | None:intergenic | 55.0% |
| CGTCGTCTGGGGGCTTTACC+AGG | + | chr6_1:706339-706358 | Msa0867640:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 706303 | 706908 | 706303 | ID=Msa0867640;Name=Msa0867640 |
| chr6_1 | mRNA | 706303 | 706908 | 706303 | ID=Msa0867640-mRNA-1;Parent=Msa0867640;Name=Msa0867640-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201 |
| chr6_1 | exon | 706303 | 706908 | 706303 | ID=Msa0867640-mRNA-1:exon:204;Parent=Msa0867640-mRNA-1 |
| chr6_1 | CDS | 706303 | 706908 | 706303 | ID=Msa0867640-mRNA-1:cds;Parent=Msa0867640-mRNA-1 |
| Gene Sequence |
| Protein sequence |
