AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1366010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366010 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366010 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366010 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366010 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366010 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366010 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1366010 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366010 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018442:-110233 Msa1366010:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018442:+110253 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018442:-110264 Msa1366010:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018442:-110564 Msa1366010:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018442:-110508 Msa1366010:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018442:-110205 Msa1366010:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018442:+110293 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018442:-110707 Msa1366010:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018442:+110384 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018442:+110258 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018442:+110439 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018442:-110232 Msa1366010:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018442:-110228 Msa1366010:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018442:+110684 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018442:+110279 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018442:-110543 Msa1366010:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018442:+110440 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018442:+110705 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018442:+110292 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018442:-110364 Msa1366010:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018442:+110366 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018442:+110524 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018442:-110696 Msa1366010:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018442:-110363 Msa1366010:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018442:+110447 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018442:+110274 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018442:+110296 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018442:+110294 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018442:-110764 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018442:-110765 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018442:-110552 Msa1366010:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018442:+110424 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018442:+110295 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0018442:110702-110721 Msa1366010:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0018442:110703-110722 Msa1366010:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0018442:110730-110749 Msa1366010:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0018442:110329-110348 Msa1366010:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0018442:110414-110433 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0018442:110449-110468 Msa1366010:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0018442:110680-110699 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0018442:110685-110704 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0018442:110707-110726 Msa1366010:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0018442:110199-110218 Msa1366010:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0018442:110200-110219 Msa1366010:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0018442:110427-110446 Msa1366010:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0018442:110448-110467 Msa1366010:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0018442:110514-110533 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0018442:110512-110531 Msa1366010:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0018442:110572-110591 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0018442:110571-110590 Msa1366010:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0018442:110572-110591 Msa1366010:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0018442:110671-110690 Msa1366010:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0018442:110197-110216 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0018442:110201-110220 Msa1366010:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0018442:110228-110247 Msa1366010:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0018442:110454-110473 Msa1366010:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0018442:110498-110517 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0018442:110499-110518 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0018442:110202-110221 Msa1366010:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0018442:110280-110299 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0018442:110371-110390 Msa1366010:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0018442:110491-110510 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0018442:110554-110573 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0018442:110646-110665 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0018442:110659-110678 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0018442:110664-110683 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0018442:110239-110258 Msa1366010:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0018442:110254-110273 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0018442:110392-110411 Msa1366010:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0018442:110645-110664 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0018442:110233-110252 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0018442:110383-110402 Msa1366010:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0018442:110644-110663 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0018442:110642-110661 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0018442:110643-110662 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0018442:110212-110231 Msa1366010:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018442 gene 110176 110781 110176 ID=Msa1366010;Name=Msa1366010
tig0018442 mRNA 110176 110781 110176 ID=Msa1366010-mRNA-1;Parent=Msa1366010;Name=Msa1366010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018442 exon 110176 110781 110176 ID=Msa1366010-mRNA-1:exon:14936;Parent=Msa1366010-mRNA-1
tig0018442 CDS 110176 110781 110176 ID=Msa1366010-mRNA-1:cds;Parent=Msa1366010-mRNA-1
Gene Sequence

>Msa1366010

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1366010

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*