AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354350


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354350 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354350 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354350 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354350 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354350 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354350 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1354350 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354350 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0014543:-130715 Msa1354350:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0014543:+130735 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0014543:-130746 Msa1354350:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0014543:-131046 Msa1354350:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0014543:-130990 Msa1354350:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0014543:-130687 Msa1354350:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0014543:+130775 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0014543:-131189 Msa1354350:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0014543:+130866 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0014543:+130740 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0014543:+130921 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0014543:-130714 Msa1354350:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0014543:-130710 Msa1354350:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0014543:+131166 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0014543:+130761 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0014543:-131025 Msa1354350:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0014543:+130922 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0014543:+131187 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0014543:+130774 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0014543:-130846 Msa1354350:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0014543:+130848 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0014543:+131006 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0014543:-131178 Msa1354350:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0014543:-130845 Msa1354350:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0014543:+130929 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0014543:+130756 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0014543:+130778 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0014543:+130776 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0014543:-131246 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0014543:-131247 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0014543:-131034 Msa1354350:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0014543:+130906 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0014543:+130777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0014543:131184-131203 Msa1354350:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0014543:131185-131204 Msa1354350:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0014543:131212-131231 Msa1354350:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0014543:130811-130830 Msa1354350:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0014543:130896-130915 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0014543:130931-130950 Msa1354350:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0014543:131162-131181 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0014543:131167-131186 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0014543:131189-131208 Msa1354350:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0014543:130681-130700 Msa1354350:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0014543:130682-130701 Msa1354350:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0014543:130909-130928 Msa1354350:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0014543:130930-130949 Msa1354350:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0014543:130996-131015 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0014543:130994-131013 Msa1354350:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0014543:131054-131073 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0014543:131053-131072 Msa1354350:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0014543:131054-131073 Msa1354350:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0014543:131153-131172 Msa1354350:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0014543:130679-130698 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0014543:130683-130702 Msa1354350:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0014543:130710-130729 Msa1354350:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0014543:130936-130955 Msa1354350:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0014543:130980-130999 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0014543:130981-131000 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0014543:130684-130703 Msa1354350:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0014543:130762-130781 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0014543:130853-130872 Msa1354350:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0014543:130973-130992 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0014543:131036-131055 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0014543:131128-131147 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0014543:131141-131160 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0014543:131146-131165 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0014543:130721-130740 Msa1354350:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0014543:130736-130755 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0014543:130874-130893 Msa1354350:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0014543:131127-131146 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0014543:130715-130734 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0014543:130865-130884 Msa1354350:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0014543:131126-131145 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0014543:131124-131143 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0014543:131125-131144 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0014543:130694-130713 Msa1354350:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 130658 131263 130658 ID=Msa1354350;Name=Msa1354350
tig0014543 mRNA 130658 131263 130658 ID=Msa1354350-mRNA-1;Parent=Msa1354350;Name=Msa1354350-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0014543 exon 130658 131263 130658 ID=Msa1354350-mRNA-1:exon:9098;Parent=Msa1354350-mRNA-1
tig0014543 CDS 130658 131263 130658 ID=Msa1354350-mRNA-1:cds;Parent=Msa1354350-mRNA-1
Gene Sequence

>Msa1354350

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1354350

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*