AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1380640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380640 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1380640 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1380640 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1380640 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1380640 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1380640 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1380640 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1380640 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0020386:-56526 Msa1380640:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0020386:+56546 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0020386:-56557 Msa1380640:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0020386:-56857 Msa1380640:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0020386:-56801 Msa1380640:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0020386:-56498 Msa1380640:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0020386:+56586 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0020386:-57000 Msa1380640:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0020386:+56677 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0020386:+56551 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0020386:+56732 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0020386:-56525 Msa1380640:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0020386:-56521 Msa1380640:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0020386:+56977 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0020386:+56572 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0020386:-56836 Msa1380640:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0020386:+56733 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0020386:+56998 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0020386:+56585 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0020386:-56657 Msa1380640:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0020386:+56659 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0020386:+56817 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0020386:-56989 Msa1380640:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0020386:-56656 Msa1380640:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0020386:+56740 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0020386:+56567 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0020386:+56589 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0020386:+56587 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0020386:-57057 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0020386:-57058 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0020386:-56845 Msa1380640:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0020386:+56717 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0020386:+56588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0020386:56995-57014 Msa1380640:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0020386:56996-57015 Msa1380640:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0020386:57023-57042 Msa1380640:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0020386:56622-56641 Msa1380640:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0020386:56707-56726 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0020386:56742-56761 Msa1380640:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0020386:56973-56992 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0020386:56978-56997 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0020386:57000-57019 Msa1380640:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0020386:56492-56511 Msa1380640:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0020386:56493-56512 Msa1380640:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0020386:56720-56739 Msa1380640:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0020386:56741-56760 Msa1380640:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0020386:56807-56826 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0020386:56805-56824 Msa1380640:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0020386:56865-56884 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0020386:56864-56883 Msa1380640:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0020386:56865-56884 Msa1380640:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0020386:56964-56983 Msa1380640:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0020386:56490-56509 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0020386:56494-56513 Msa1380640:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0020386:56521-56540 Msa1380640:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0020386:56747-56766 Msa1380640:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0020386:56791-56810 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0020386:56792-56811 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0020386:56495-56514 Msa1380640:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0020386:56573-56592 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0020386:56664-56683 Msa1380640:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0020386:56784-56803 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0020386:56847-56866 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0020386:56939-56958 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0020386:56952-56971 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0020386:56957-56976 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0020386:56532-56551 Msa1380640:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0020386:56547-56566 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0020386:56685-56704 Msa1380640:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0020386:56938-56957 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0020386:56526-56545 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0020386:56676-56695 Msa1380640:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0020386:56937-56956 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0020386:56935-56954 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0020386:56936-56955 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0020386:56505-56524 Msa1380640:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0020386 gene 56469 57074 56469 ID=Msa1380640;Name=Msa1380640
tig0020386 mRNA 56469 57074 56469 ID=Msa1380640-mRNA-1;Parent=Msa1380640;Name=Msa1380640-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0020386 exon 56469 57074 56469 ID=Msa1380640-mRNA-1:exon:7180;Parent=Msa1380640-mRNA-1
tig0020386 CDS 56469 57074 56469 ID=Msa1380640-mRNA-1:cds;Parent=Msa1380640-mRNA-1
Gene Sequence

>Msa1380640

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1380640

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*