AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1366050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366050 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366050 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366050 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366050 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366050 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1366050 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1366050 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366050 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018442:-194498 Msa1366050:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018442:+194518 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018442:-194529 Msa1366050:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018442:-194829 Msa1366050:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018442:-194773 Msa1366050:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018442:-194470 Msa1366050:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018442:+194558 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018442:-194972 Msa1366050:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018442:+194649 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018442:+194523 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018442:+194704 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018442:-194497 Msa1366050:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018442:-194493 Msa1366050:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018442:+194949 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018442:+194544 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018442:-194808 Msa1366050:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018442:+194705 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018442:+194970 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018442:+194557 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018442:-194629 Msa1366050:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018442:+194631 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018442:+194789 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018442:-194961 Msa1366050:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018442:-194628 Msa1366050:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018442:+194712 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018442:+194539 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018442:+194561 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018442:+194559 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018442:-195029 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018442:-195030 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018442:-194817 Msa1366050:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018442:+194689 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018442:+194560 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0018442:194967-194986 Msa1366050:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0018442:194968-194987 Msa1366050:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0018442:194995-195014 Msa1366050:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0018442:194594-194613 Msa1366050:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0018442:194679-194698 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0018442:194714-194733 Msa1366050:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0018442:194945-194964 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0018442:194950-194969 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0018442:194972-194991 Msa1366050:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0018442:194464-194483 Msa1366050:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0018442:194465-194484 Msa1366050:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0018442:194692-194711 Msa1366050:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0018442:194713-194732 Msa1366050:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0018442:194779-194798 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0018442:194777-194796 Msa1366050:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0018442:194837-194856 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0018442:194836-194855 Msa1366050:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0018442:194837-194856 Msa1366050:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0018442:194936-194955 Msa1366050:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0018442:194462-194481 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0018442:194466-194485 Msa1366050:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0018442:194493-194512 Msa1366050:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0018442:194719-194738 Msa1366050:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0018442:194763-194782 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0018442:194764-194783 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0018442:194467-194486 Msa1366050:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0018442:194545-194564 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0018442:194636-194655 Msa1366050:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0018442:194756-194775 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0018442:194819-194838 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0018442:194911-194930 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0018442:194924-194943 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0018442:194929-194948 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0018442:194504-194523 Msa1366050:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0018442:194519-194538 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0018442:194657-194676 Msa1366050:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0018442:194910-194929 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0018442:194498-194517 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0018442:194648-194667 Msa1366050:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0018442:194909-194928 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0018442:194907-194926 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0018442:194908-194927 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0018442:194477-194496 Msa1366050:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018442 gene 194441 195046 194441 ID=Msa1366050;Name=Msa1366050
tig0018442 mRNA 194441 195046 194441 ID=Msa1366050-mRNA-1;Parent=Msa1366050;Name=Msa1366050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018442 exon 194441 195046 194441 ID=Msa1366050-mRNA-1:exon:14937;Parent=Msa1366050-mRNA-1
tig0018442 CDS 194441 195046 194441 ID=Msa1366050-mRNA-1:cds;Parent=Msa1366050-mRNA-1
Gene Sequence

>Msa1366050

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1366050

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*