AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354380 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354380 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354380 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354380 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354380 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354380 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1354380 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354380 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0014543:+211721 Msa1354380:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0014543:-211701 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0014543:+211690 Msa1354380:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0014543:+211390 Msa1354380:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0014543:+211446 Msa1354380:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0014543:+211749 Msa1354380:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0014543:-211661 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0014543:+211247 Msa1354380:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0014543:-211570 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0014543:-211696 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0014543:-211515 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0014543:+211722 Msa1354380:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0014543:+211726 Msa1354380:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0014543:-211270 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0014543:-211675 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0014543:+211411 Msa1354380:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0014543:-211514 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0014543:-211249 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0014543:-211662 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0014543:+211590 Msa1354380:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0014543:-211588 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0014543:-211430 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0014543:+211258 Msa1354380:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0014543:+211591 Msa1354380:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0014543:-211507 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0014543:-211680 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0014543:-211658 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0014543:-211660 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0014543:+211190 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0014543:+211189 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0014543:+211402 Msa1354380:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0014543:-211530 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0014543:-211659 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG + tig0014543:211721-211740 Msa1354380:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG + tig0014543:211722-211741 Msa1354380:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG + tig0014543:211749-211768 Msa1354380:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG + tig0014543:211348-211367 Msa1354380:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG - tig0014543:211433-211452 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG + tig0014543:211468-211487 Msa1354380:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG - tig0014543:211699-211718 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG - tig0014543:211704-211723 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG + tig0014543:211726-211745 Msa1354380:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG + tig0014543:211218-211237 Msa1354380:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG + tig0014543:211219-211238 Msa1354380:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG + tig0014543:211446-211465 Msa1354380:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG + tig0014543:211467-211486 Msa1354380:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG - tig0014543:211533-211552 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG + tig0014543:211531-211550 Msa1354380:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG - tig0014543:211591-211610 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG + tig0014543:211590-211609 Msa1354380:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG + tig0014543:211591-211610 Msa1354380:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG + tig0014543:211690-211709 Msa1354380:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG - tig0014543:211216-211235 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG + tig0014543:211220-211239 Msa1354380:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG + tig0014543:211247-211266 Msa1354380:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG + tig0014543:211473-211492 Msa1354380:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG - tig0014543:211517-211536 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG - tig0014543:211518-211537 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG + tig0014543:211221-211240 Msa1354380:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG - tig0014543:211299-211318 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG + tig0014543:211390-211409 Msa1354380:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG - tig0014543:211510-211529 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG - tig0014543:211573-211592 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG - tig0014543:211665-211684 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG - tig0014543:211678-211697 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG - tig0014543:211683-211702 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG + tig0014543:211258-211277 Msa1354380:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG - tig0014543:211273-211292 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG + tig0014543:211411-211430 Msa1354380:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG - tig0014543:211664-211683 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG - tig0014543:211252-211271 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG + tig0014543:211402-211421 Msa1354380:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG - tig0014543:211663-211682 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG - tig0014543:211661-211680 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG - tig0014543:211662-211681 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG + tig0014543:211231-211250 Msa1354380:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 211195 211800 211195 ID=Msa1354380;Name=Msa1354380
tig0014543 mRNA 211195 211800 211195 ID=Msa1354380-mRNA-1;Parent=Msa1354380;Name=Msa1354380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0014543 exon 211195 211800 211195 ID=Msa1354380-mRNA-1:exon:9104;Parent=Msa1354380-mRNA-1
tig0014543 CDS 211195 211800 211195 ID=Msa1354380-mRNA-1:cds;Parent=Msa1354380-mRNA-1
Gene Sequence

>Msa1354380

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1354380

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*