AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368290 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368290 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368290 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368290 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368290 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368290 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1368290 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368290 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018631:+125389 Msa1368290:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018631:-125369 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018631:+125358 Msa1368290:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018631:+125058 Msa1368290:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018631:+125114 Msa1368290:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018631:+125417 Msa1368290:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018631:-125329 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018631:+124915 Msa1368290:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018631:-125238 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018631:-125364 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018631:-125183 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018631:+125390 Msa1368290:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018631:+125394 Msa1368290:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018631:-124938 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018631:-125343 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018631:+125079 Msa1368290:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018631:-125182 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018631:-124917 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018631:-125330 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018631:+125258 Msa1368290:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018631:-125256 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018631:-125098 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018631:+124926 Msa1368290:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018631:+125259 Msa1368290:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018631:-125175 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018631:-125348 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018631:-125326 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018631:-125328 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018631:+124858 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018631:+124857 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018631:+125070 Msa1368290:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018631:-125198 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018631:-125327 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG + tig0018631:125389-125408 Msa1368290:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG + tig0018631:125390-125409 Msa1368290:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG + tig0018631:125417-125436 Msa1368290:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG + tig0018631:125016-125035 Msa1368290:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG - tig0018631:125101-125120 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG + tig0018631:125136-125155 Msa1368290:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG - tig0018631:125367-125386 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG - tig0018631:125372-125391 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG + tig0018631:125394-125413 Msa1368290:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG + tig0018631:124886-124905 Msa1368290:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG + tig0018631:124887-124906 Msa1368290:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG + tig0018631:125114-125133 Msa1368290:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG + tig0018631:125135-125154 Msa1368290:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG - tig0018631:125201-125220 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG + tig0018631:125199-125218 Msa1368290:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG - tig0018631:125259-125278 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG + tig0018631:125258-125277 Msa1368290:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG + tig0018631:125259-125278 Msa1368290:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG + tig0018631:125358-125377 Msa1368290:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG - tig0018631:124884-124903 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG + tig0018631:124888-124907 Msa1368290:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG + tig0018631:124915-124934 Msa1368290:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG + tig0018631:125141-125160 Msa1368290:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG - tig0018631:125185-125204 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG - tig0018631:125186-125205 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG + tig0018631:124889-124908 Msa1368290:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG - tig0018631:124967-124986 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG + tig0018631:125058-125077 Msa1368290:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG - tig0018631:125178-125197 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG - tig0018631:125241-125260 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG - tig0018631:125333-125352 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG - tig0018631:125346-125365 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG - tig0018631:125351-125370 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG + tig0018631:124926-124945 Msa1368290:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG - tig0018631:124941-124960 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG + tig0018631:125079-125098 Msa1368290:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG - tig0018631:125332-125351 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG - tig0018631:124920-124939 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG + tig0018631:125070-125089 Msa1368290:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG - tig0018631:125331-125350 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG - tig0018631:125329-125348 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG - tig0018631:125330-125349 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG + tig0018631:124899-124918 Msa1368290:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018631 gene 124863 125468 124863 ID=Msa1368290;Name=Msa1368290
tig0018631 mRNA 124863 125468 124863 ID=Msa1368290-mRNA-1;Parent=Msa1368290;Name=Msa1368290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018631 exon 124863 125468 124863 ID=Msa1368290-mRNA-1:exon:399;Parent=Msa1368290-mRNA-1
tig0018631 CDS 124863 125468 124863 ID=Msa1368290-mRNA-1:cds;Parent=Msa1368290-mRNA-1
Gene Sequence

>Msa1368290

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1368290

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*