AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1269420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1269420 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1269420 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1269420 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1269420 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1269420 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1269420 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1269420 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1269420 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 8_3:-2642756 Msa1269420:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 8_3:+2642776 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 8_3:-2642787 Msa1269420:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 8_3:-2643087 Msa1269420:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 8_3:-2643031 Msa1269420:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 8_3:-2642728 Msa1269420:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 8_3:+2642816 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 8_3:-2643230 Msa1269420:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 8_3:+2642907 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 8_3:+2642781 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 8_3:+2642962 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 8_3:-2642755 Msa1269420:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 8_3:-2642751 Msa1269420:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 8_3:+2643207 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 8_3:+2642802 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 8_3:-2643066 Msa1269420:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 8_3:+2642963 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 8_3:+2643228 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 8_3:+2642815 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 8_3:-2642887 Msa1269420:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 8_3:+2642889 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 8_3:+2643047 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 8_3:-2643219 Msa1269420:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 8_3:-2642886 Msa1269420:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 8_3:+2642970 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 8_3:+2642797 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 8_3:+2642819 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 8_3:+2642817 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 8_3:-2643287 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 8_3:-2643288 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 8_3:-2643075 Msa1269420:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 8_3:+2642947 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 8_3:+2642818 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - chr8_3:2643225-2643244 Msa1269420:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - chr8_3:2643226-2643245 Msa1269420:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - chr8_3:2643253-2643272 Msa1269420:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - chr8_3:2642852-2642871 Msa1269420:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + chr8_3:2642937-2642956 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - chr8_3:2642972-2642991 Msa1269420:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + chr8_3:2643203-2643222 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + chr8_3:2643208-2643227 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - chr8_3:2643230-2643249 Msa1269420:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - chr8_3:2642722-2642741 Msa1269420:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - chr8_3:2642723-2642742 Msa1269420:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - chr8_3:2642950-2642969 Msa1269420:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - chr8_3:2642971-2642990 Msa1269420:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + chr8_3:2643037-2643056 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - chr8_3:2643035-2643054 Msa1269420:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + chr8_3:2643095-2643114 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - chr8_3:2643094-2643113 Msa1269420:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - chr8_3:2643095-2643114 Msa1269420:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - chr8_3:2643194-2643213 Msa1269420:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + chr8_3:2642720-2642739 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - chr8_3:2642724-2642743 Msa1269420:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - chr8_3:2642751-2642770 Msa1269420:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - chr8_3:2642977-2642996 Msa1269420:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + chr8_3:2643021-2643040 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + chr8_3:2643022-2643041 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - chr8_3:2642725-2642744 Msa1269420:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + chr8_3:2642803-2642822 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - chr8_3:2642894-2642913 Msa1269420:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + chr8_3:2643014-2643033 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + chr8_3:2643077-2643096 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + chr8_3:2643169-2643188 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + chr8_3:2643182-2643201 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + chr8_3:2643187-2643206 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - chr8_3:2642762-2642781 Msa1269420:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + chr8_3:2642777-2642796 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - chr8_3:2642915-2642934 Msa1269420:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + chr8_3:2643168-2643187 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + chr8_3:2642756-2642775 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - chr8_3:2642906-2642925 Msa1269420:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + chr8_3:2643167-2643186 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + chr8_3:2643165-2643184 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + chr8_3:2643166-2643185 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - chr8_3:2642735-2642754 Msa1269420:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 2642699 2643304 2642699 ID=Msa1269420;Name=Msa1269420
chr8_3 mRNA 2642699 2643304 2642699 ID=Msa1269420-mRNA-1;Parent=Msa1269420;Name=Msa1269420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
chr8_3 exon 2642699 2643304 2642699 ID=Msa1269420-mRNA-1:exon:843;Parent=Msa1269420-mRNA-1
chr8_3 CDS 2642699 2643304 2642699 ID=Msa1269420-mRNA-1:cds;Parent=Msa1269420-mRNA-1
Gene Sequence

>Msa1269420

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1269420

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*