AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368270 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368270 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368270 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368270 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368270 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368270 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1368270 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368270 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018631:+46456 Msa1368270:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018631:-46436 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018631:+46425 Msa1368270:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018631:+46125 Msa1368270:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018631:+46181 Msa1368270:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018631:+46484 Msa1368270:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018631:-46396 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018631:+45982 Msa1368270:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018631:-46305 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018631:-46431 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018631:-46250 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018631:+46457 Msa1368270:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018631:+46461 Msa1368270:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018631:-46005 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018631:-46410 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018631:+46146 Msa1368270:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018631:-46249 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018631:-45984 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018631:-46397 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018631:+46325 Msa1368270:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018631:-46323 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018631:-46165 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018631:+45993 Msa1368270:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018631:+46326 Msa1368270:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018631:-46242 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018631:-46415 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018631:-46393 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018631:-46395 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018631:+45925 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018631:+45924 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018631:+46137 Msa1368270:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018631:-46265 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018631:-46394 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG + tig0018631:46456-46475 Msa1368270:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG + tig0018631:46457-46476 Msa1368270:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG + tig0018631:46484-46503 Msa1368270:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG + tig0018631:46083-46102 Msa1368270:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG - tig0018631:46168-46187 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG + tig0018631:46203-46222 Msa1368270:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG - tig0018631:46434-46453 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG - tig0018631:46439-46458 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG + tig0018631:46461-46480 Msa1368270:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG + tig0018631:45953-45972 Msa1368270:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG + tig0018631:45954-45973 Msa1368270:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG + tig0018631:46181-46200 Msa1368270:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG + tig0018631:46202-46221 Msa1368270:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG - tig0018631:46268-46287 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG + tig0018631:46266-46285 Msa1368270:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG - tig0018631:46326-46345 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG + tig0018631:46325-46344 Msa1368270:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG + tig0018631:46326-46345 Msa1368270:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG + tig0018631:46425-46444 Msa1368270:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG - tig0018631:45951-45970 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG + tig0018631:45955-45974 Msa1368270:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG + tig0018631:45982-46001 Msa1368270:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG + tig0018631:46208-46227 Msa1368270:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG - tig0018631:46252-46271 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG - tig0018631:46253-46272 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG + tig0018631:45956-45975 Msa1368270:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG - tig0018631:46034-46053 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG + tig0018631:46125-46144 Msa1368270:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG - tig0018631:46245-46264 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG - tig0018631:46308-46327 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG - tig0018631:46400-46419 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG - tig0018631:46413-46432 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG - tig0018631:46418-46437 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG + tig0018631:45993-46012 Msa1368270:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG - tig0018631:46008-46027 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG + tig0018631:46146-46165 Msa1368270:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG - tig0018631:46399-46418 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG - tig0018631:45987-46006 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG + tig0018631:46137-46156 Msa1368270:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG - tig0018631:46398-46417 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG - tig0018631:46396-46415 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG - tig0018631:46397-46416 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG + tig0018631:45966-45985 Msa1368270:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018631 gene 45930 46535 45930 ID=Msa1368270;Name=Msa1368270
tig0018631 mRNA 45930 46535 45930 ID=Msa1368270-mRNA-1;Parent=Msa1368270;Name=Msa1368270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018631 exon 45930 46535 45930 ID=Msa1368270-mRNA-1:exon:383;Parent=Msa1368270-mRNA-1
tig0018631 CDS 45930 46535 45930 ID=Msa1368270-mRNA-1:cds;Parent=Msa1368270-mRNA-1
Gene Sequence

>Msa1368270

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1368270

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*