Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867650 | YP_009141600.1 | 100.000 | 201 | 0 | 0 | 1 | 201 | 1 | 201 | 3.55e-143 | 407 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867650 | sp|Q9BBT5|RR4_LOTJA | 95.522 | 201 | 9 | 0 | 1 | 201 | 1 | 201 | 8.95e-140 | 391 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867650 | A0A0F6NLD0 | 100.000 | 201 | 0 | 0 | 1 | 201 | 1 | 201 | 1.70e-143 | 407 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0095170 | Msa0867650 | 0.802945 | 4.409236e-49 | -8.615850e-47 |
| Msa0095190 | Msa0867650 | 0.820365 | 7.127215e-53 | -8.615850e-47 |
| Msa0139800 | Msa0867650 | 0.802137 | 6.470567e-49 | -8.615850e-47 |
| Msa0268170 | Msa0867650 | 0.802592 | 5.213449e-49 | -8.615850e-47 |
| Msa0268390 | Msa0867650 | 0.801490 | 8.785555e-49 | -8.615850e-47 |
| Msa0268400 | Msa0867650 | 0.810309 | 1.228850e-50 | -8.615850e-47 |
| Msa0268410 | Msa0867650 | 0.810094 | 1.367082e-50 | -8.615850e-47 |
| Msa0268550 | Msa0867650 | 0.820444 | 6.838140e-53 | -8.615850e-47 |
| Msa0268580 | Msa0867650 | 0.805231 | 1.474972e-49 | -8.615850e-47 |
| Msa0268640 | Msa0867650 | 0.813967 | 1.957136e-51 | -8.615850e-47 |
| Msa0268720 | Msa0867650 | 0.803205 | 3.894897e-49 | -8.615850e-47 |
| Msa0268840 | Msa0867650 | 0.814873 | 1.234356e-51 | -8.615850e-47 |
| Msa0268940 | Msa0867650 | 0.802427 | 5.640419e-49 | -8.615850e-47 |
| Msa0269040 | Msa0867650 | 0.800228 | 1.590113e-48 | -8.615850e-47 |
| Msa0269050 | Msa0867650 | 0.808572 | 2.899802e-50 | -8.615850e-47 |
| Msa0344180 | Msa0867650 | 0.809320 | 2.006056e-50 | -8.615850e-47 |
| Msa0344190 | Msa0867650 | 0.804758 | 1.852463e-49 | -8.615850e-47 |
| Msa0344300 | Msa0867650 | 0.824746 | 6.829361e-54 | -8.615850e-47 |
| Msa0344330 | Msa0867650 | 0.806900 | 6.570568e-50 | -8.615850e-47 |
| Msa0582510 | Msa0867650 | 0.802230 | 6.191695e-49 | -8.615850e-47 |
| Msa0582520 | Msa0867650 | 0.810367 | 1.194253e-50 | -8.615850e-47 |
| Msa0582630 | Msa0867650 | 0.806189 | 9.284087e-50 | -8.615850e-47 |
| Msa0582660 | Msa0867650 | 0.804914 | 1.718256e-49 | -8.615850e-47 |
| Msa0582690 | Msa0867650 | 0.800986 | 1.114308e-48 | -8.615850e-47 |
| Msa0582700 | Msa0867650 | 0.813261 | 2.799074e-51 | -8.615850e-47 |
| Msa0582750 | Msa0867650 | 0.807412 | 5.119493e-50 | -8.615850e-47 |
| Msa0582780 | Msa0867650 | 0.808077 | 3.698248e-50 | -8.615850e-47 |
| Msa0582820 | Msa0867650 | 0.801085 | 1.063245e-48 | -8.615850e-47 |
| Msa0867640 | Msa0867650 | 0.836613 | 8.445519e-57 | -8.615850e-47 |
| Msa0867650 | Msa0931070 | 0.800860 | 1.181916e-48 | -8.615850e-47 |
| Msa0867650 | Msa0931080 | 0.808645 | 2.797325e-50 | -8.615850e-47 |
| Msa0867650 | Msa0931130 | 0.803934 | 2.749786e-49 | -8.615850e-47 |
| Msa0867650 | Msa0931140 | 0.807664 | 4.526788e-50 | -8.615850e-47 |
| Msa0867650 | Msa0931190 | 0.800699 | 1.274728e-48 | -8.615850e-47 |
| Msa0867650 | Msa0931210 | 0.808307 | 3.303163e-50 | -8.615850e-47 |
| Msa0867650 | Msa0931240 | 0.808048 | 3.749938e-50 | -8.615850e-47 |
| Msa0867650 | Msa0931260 | 0.802507 | 5.428201e-49 | -8.615850e-47 |
| Msa0867650 | Msa0983720 | 0.811024 | 8.607722e-51 | -8.615850e-47 |
| Msa0867650 | Msa1086070 | 0.803364 | 3.611441e-49 | -8.615850e-47 |
| Msa0867650 | Msa1086150 | 0.812742 | 3.637592e-51 | -8.615850e-47 |
| Msa0867650 | Msa1086180 | 0.812263 | 4.629665e-51 | -8.615850e-47 |
| Msa0867650 | Msa1086210 | 0.801016 | 1.098392e-48 | -8.615850e-47 |
| Msa0867650 | Msa1086280 | 0.802773 | 4.784766e-49 | -8.615850e-47 |
| Msa0867650 | Msa1086380 | 0.806222 | 9.133986e-50 | -8.615850e-47 |
| Msa0867650 | Msa1086450 | 0.822846 | 1.903863e-53 | -8.615850e-47 |
| Msa0867650 | Msa1086470 | 0.807790 | 4.255651e-50 | -8.615850e-47 |
| Msa0867650 | Msa1086880 | 0.807937 | 3.959566e-50 | -8.615850e-47 |
| Msa0867650 | Msa1086910 | 0.801456 | 8.927074e-49 | -8.615850e-47 |
| Msa0867650 | Msa1143120 | 0.800392 | 1.472593e-48 | -8.615850e-47 |
| Msa0867650 | Msa1143130 | 0.815600 | 8.508211e-52 | -8.615850e-47 |
| Msa0867650 | Msa1269400 | 0.808190 | 3.498731e-50 | -8.615850e-47 |
| Msa0867650 | Msa1269410 | 0.809244 | 2.082129e-50 | -8.615850e-47 |
| Msa0867650 | Msa1269420 | 0.855857 | 4.761420e-62 | -8.615850e-47 |
| Msa0867650 | Msa1354320 | 0.802104 | 6.571236e-49 | -8.615850e-47 |
| Msa0867650 | Msa1354350 | 0.848852 | 4.706584e-60 | -8.615850e-47 |
| Msa0867650 | Msa1354380 | 0.854641 | 1.074858e-61 | -8.615850e-47 |
| Msa0867650 | Msa1354390 | 0.867657 | 1.164162e-65 | -8.615850e-47 |
| Msa0867650 | Msa1354900 | 0.812394 | 4.334217e-51 | -8.615850e-47 |
| Msa0867650 | Msa1354930 | 0.801536 | 8.598996e-49 | -8.615850e-47 |
| Msa0867650 | Msa1354950 | 0.842046 | 3.283090e-58 | -8.615850e-47 |
| Msa0867650 | Msa1355070 | 0.810188 | 1.304751e-50 | -8.615850e-47 |
| Msa0867650 | Msa1366000 | 0.821082 | 4.878817e-53 | -8.615850e-47 |
| Msa0867650 | Msa1366010 | 0.833083 | 6.538489e-56 | -8.615850e-47 |
| Msa0867650 | Msa1366050 | 0.843787 | 1.130100e-58 | -8.615850e-47 |
| Msa0867650 | Msa1368270 | 0.856873 | 2.396773e-62 | -8.615850e-47 |
| Msa0867650 | Msa1368280 | 0.814072 | 1.856007e-51 | -8.615850e-47 |
| Msa0867650 | Msa1368290 | 0.864989 | 8.171499e-65 | -8.615850e-47 |
| Msa0867650 | Msa1368300 | 0.844190 | 8.813047e-59 | -8.615850e-47 |
| Msa0867650 | Msa1380590 | 0.800097 | 1.691153e-48 | -8.615850e-47 |
| Msa0867650 | Msa1380620 | 0.803604 | 3.220524e-49 | -8.615850e-47 |
| Msa0867650 | Msa1380640 | 0.840387 | 8.966278e-58 | -8.615850e-47 |
| Msa0867650 | Msa1380710 | 0.822844 | 1.906244e-53 | -8.615850e-47 |
| Msa0867650 | Msa1385370 | 0.809197 | 2.131811e-50 | -8.615850e-47 |
| Msa0867650 | Msa1394040 | 0.801283 | 9.684444e-49 | -8.615850e-47 |
| Msa0867650 | Msa1394060 | 0.804278 | 2.333047e-49 | -8.615850e-47 |
| Msa0867650 | Msa1394230 | 0.829549 | 4.836651e-55 | -8.615850e-47 |
| Msa0867650 | Msa1394250 | 0.802520 | 5.397013e-49 | -8.615850e-47 |
| Msa0867650 | Msa1398700 | 0.820813 | 5.624541e-53 | -8.615850e-47 |
| Msa0867650 | Msa1398710 | 0.817791 | 2.745362e-52 | -8.615850e-47 |
| Msa0867650 | Msa1398720 | 0.850364 | 1.781712e-60 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867650 | MtrunA17_Chr7g0229301 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867650 | MtrunA17_Chr6g0454441 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867650 | MtrunA17_Chr4g0016451 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867650 | MtrunA17_Chr6g0466281 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867650 | MtrunA17_CPg0493311 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.37e-143 | 398 |
| Msa0867650 | MtrunA17_Chr7g0229661 | 84.080 | 201 | 13 | 1 | 1 | 201 | 1 | 182 | 1.89e-115 | 327 |
| Msa0867650 | MtrunA17_Chr4g0015291 | 97.436 | 156 | 4 | 0 | 1 | 156 | 1 | 156 | 3.32e-108 | 307 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867650 | ATCG00380.1 | 88.060 | 201 | 24 | 0 | 1 | 201 | 1 | 201 | 2.12e-126 | 355 |
Find 33 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAATAATAGATAGTAAA+TGG | 0.189787 | 6_1:-717927 | Msa0867650:CDS |
| GATTGACTAATCCTTTATAT+TGG | 0.284280 | 6_1:+717947 | None:intergenic |
| CTTGATCCATTCCAATATAA+AGG | 0.291752 | 6_1:-717958 | Msa0867650:CDS |
| CTTAAATACGTTCGTATCGC+TGG | 0.342652 | 6_1:-718258 | Msa0867650:CDS |
| ACAATTACTTGAAATGCGTT+TGG | 0.359489 | 6_1:-718202 | Msa0867650:CDS |
| TTTGAAAATTAATGAATTGC+TGG | 0.365999 | 6_1:-717899 | Msa0867650:CDS |
| ATGGATTGGCAATTCTTCGC+GGG | 0.450528 | 6_1:+717987 | None:intergenic |
| TACCAGGACTAACTAGTAAA+CGG | 0.463029 | 6_1:-718401 | Msa0867650:CDS |
| TGAGGTTTGCAACGATAACT+TGG | 0.468099 | 6_1:+718078 | None:intergenic |
| ACTAATCCTTTATATTGGAA+TGG | 0.475526 | 6_1:+717952 | None:intergenic |
| GTCTGTGGTTAACTAATTGA+CGG | 0.477609 | 6_1:+718133 | None:intergenic |
| TCAAATAATAGATAGTAAAT+GGG | 0.487910 | 6_1:-717926 | Msa0867650:CDS |
| ATAATAGATAGTAAATGGGT+TGG | 0.504615 | 6_1:-717922 | Msa0867650:CDS |
| CTAAGTTCGCTTCCAACTGT+AGG | 0.508663 | 6_1:+718378 | None:intergenic |
| GGATCAAGAGTCAAATGGAT+TGG | 0.511159 | 6_1:+717973 | None:intergenic |
| GGAAAAGCGAAAGGTTCAAC+CGG | 0.516182 | 6_1:-718237 | Msa0867650:CDS |
| TCTGTGGTTAACTAATTGAC+GGG | 0.521261 | 6_1:+718134 | None:intergenic |
| GGCCGTTTACTAGTTAGTCC+TGG | 0.527000 | 6_1:+718399 | None:intergenic |
| AATGGATTGGCAATTCTTCG+CGG | 0.545268 | 6_1:+717986 | None:intergenic |
| TCAAGATATTATTACAGCGA+AGG | 0.559737 | 6_1:-718058 | Msa0867650:CDS |
| TTCGCTGTAATAATATCTTG+AGG | 0.560954 | 6_1:+718060 | None:intergenic |
| TAATTGTAGTAAAACTTGAC+CGG | 0.568250 | 6_1:+718218 | None:intergenic |
| ACTAGTAAACGGCCTACAGT+TGG | 0.568372 | 6_1:-718390 | Msa0867650:CDS |
| CAAGATATTATTACAGCGAA+GGG | 0.569002 | 6_1:-718057 | Msa0867650:CDS |
| TTAACTAATTGACGGGCTTG+AGG | 0.581554 | 6_1:+718141 | None:intergenic |
| GGAATGGATCAAGAGTCAAA+TGG | 0.582974 | 6_1:+717968 | None:intergenic |
| GATTGGCAATTCTTCGCGGG+GGG | 0.584547 | 6_1:+717990 | None:intergenic |
| TGGATTGGCAATTCTTCGCG+GGG | 0.610754 | 6_1:+717988 | None:intergenic |
| TGTTTATGTCACGTTACAGA+GGG | 0.632465 | 6_1:-718458 | None:intergenic |
| GTGTTTATGTCACGTTACAG+AGG | 0.633128 | 6_1:-718459 | None:intergenic |
| CGTATCGCTGGAAAAGCGAA+AGG | 0.633501 | 6_1:-718246 | Msa0867650:CDS |
| CATTAACTAAAACATGTCTG+TGG | 0.663264 | 6_1:+718118 | None:intergenic |
| GGATTGGCAATTCTTCGCGG+GGG | 0.681198 | 6_1:+717989 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAATAATAGATAGTAAA+TGG | - | chr6_1:718396-718415 | Msa0867650:CDS | 15.0% |
| !! | TCAAATAATAGATAGTAAAT+GGG | - | chr6_1:718397-718416 | Msa0867650:CDS | 15.0% |
| !! | TTTGAAAATTAATGAATTGC+TGG | - | chr6_1:718424-718443 | Msa0867650:CDS | 20.0% |
| !! | CAAAAATTGCGTTTTCATTA+TGG | - | chr6_1:718023-718042 | Msa0867650:CDS | 25.0% |
| ! | TAATTGTAGTAAAACTTGAC+CGG | + | chr6_1:718108-718127 | None:intergenic | 25.0% |
| !!! | GATAACATACTTTTTCGATT+GGG | - | chr6_1:718143-718162 | Msa0867650:CDS | 25.0% |
| ! | ACTAATCCTTTATATTGGAA+TGG | + | chr6_1:718374-718393 | None:intergenic | 25.0% |
| ! | GATTGACTAATCCTTTATAT+TGG | + | chr6_1:718379-718398 | None:intergenic | 25.0% |
| ! | ATAATAGATAGTAAATGGGT+TGG | - | chr6_1:718401-718420 | Msa0867650:CDS | 25.0% |
| TTTCAAAAAAATACGTCGTC+TGG | - | chr6_1:717893-717912 | Msa0867650:CDS | 30.0% | |
| TTCAAAAAAATACGTCGTCT+GGG | - | chr6_1:717894-717913 | Msa0867650:CDS | 30.0% | |
| ACAATTACTTGAAATGCGTT+TGG | - | chr6_1:718121-718140 | Msa0867650:CDS | 30.0% | |
| !!! | GGATAACATACTTTTTCGAT+TGG | - | chr6_1:718142-718161 | Msa0867650:CDS | 30.0% |
| CATTAACTAAAACATGTCTG+TGG | + | chr6_1:718208-718227 | None:intergenic | 30.0% | |
| !!! | CACAGACATGTTTTAGTTAA+TGG | - | chr6_1:718206-718225 | Msa0867650:CDS | 30.0% |
| TTCGCTGTAATAATATCTTG+AGG | + | chr6_1:718266-718285 | None:intergenic | 30.0% | |
| TCAAGATATTATTACAGCGA+AGG | - | chr6_1:718265-718284 | Msa0867650:CDS | 30.0% | |
| CAAGATATTATTACAGCGAA+GGG | - | chr6_1:718266-718285 | Msa0867650:CDS | 30.0% | |
| CTTGATCCATTCCAATATAA+AGG | - | chr6_1:718365-718384 | Msa0867650:CDS | 30.0% | |
| !!! | CGACGTATTTTTTTGAAACG+AGG | + | chr6_1:717891-717910 | None:intergenic | 35.0% |
| TCAAAAAAATACGTCGTCTG+GGG | - | chr6_1:717895-717914 | Msa0867650:CDS | 35.0% | |
| TACCAGGACTAACTAGTAAA+CGG | - | chr6_1:717922-717941 | Msa0867650:CDS | 35.0% | |
| !!! | CATACTTTTTCGATTGGGTA+TGG | - | chr6_1:718148-718167 | Msa0867650:CDS | 35.0% |
| TCTGTGGTTAACTAATTGAC+GGG | + | chr6_1:718192-718211 | None:intergenic | 35.0% | |
| GTCTGTGGTTAACTAATTGA+CGG | + | chr6_1:718193-718212 | None:intergenic | 35.0% | |
| CAAAAAAATACGTCGTCTGG+GGG | - | chr6_1:717896-717915 | Msa0867650:CDS | 40.0% | |
| !!! | ATTTTTTACTCGAGCGCGAT+TGG | + | chr6_1:717974-717993 | None:intergenic | 40.0% |
| CTTAAATACGTTCGTATCGC+TGG | - | chr6_1:718065-718084 | Msa0867650:CDS | 40.0% | |
| !! | TTAACTAATTGACGGGCTTG+AGG | + | chr6_1:718185-718204 | None:intergenic | 40.0% |
| TGAGGTTTGCAACGATAACT+TGG | + | chr6_1:718248-718267 | None:intergenic | 40.0% | |
| !! | AATGGATTGGCAATTCTTCG+CGG | + | chr6_1:718340-718359 | None:intergenic | 40.0% |
| GGATCAAGAGTCAAATGGAT+TGG | + | chr6_1:718353-718372 | None:intergenic | 40.0% | |
| GGAATGGATCAAGAGTCAAA+TGG | + | chr6_1:718358-718377 | None:intergenic | 40.0% | |
| ACTAGTAAACGGCCTACAGT+TGG | - | chr6_1:717933-717952 | Msa0867650:CDS | 45.0% | |
| CTAAGTTCGCTTCCAACTGT+AGG | + | chr6_1:717948-717967 | None:intergenic | 45.0% | |
| GGAAAAGCGAAAGGTTCAAC+CGG | - | chr6_1:718086-718105 | Msa0867650:CDS | 45.0% | |
| !! | ATGGATTGGCAATTCTTCGC+GGG | + | chr6_1:718339-718358 | None:intergenic | 45.0% |
| GGCCGTTTACTAGTTAGTCC+TGG | + | chr6_1:717927-717946 | None:intergenic | 50.0% | |
| CGTATCGCTGGAAAAGCGAA+AGG | - | chr6_1:718077-718096 | Msa0867650:CDS | 50.0% | |
| !! | TGGATTGGCAATTCTTCGCG+GGG | + | chr6_1:718338-718357 | None:intergenic | 50.0% |
| GATTGGCAATTCTTCGCGGG+GGG | + | chr6_1:718336-718355 | None:intergenic | 55.0% | |
| ! | GGATTGGCAATTCTTCGCGG+GGG | + | chr6_1:718337-718356 | None:intergenic | 55.0% |
| CGTCGTCTGGGGGCTTTACC+AGG | - | chr6_1:717906-717925 | Msa0867650:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 717870 | 718475 | 717870 | ID=Msa0867650;Name=Msa0867650 |
| chr6_1 | mRNA | 717870 | 718475 | 717870 | ID=Msa0867650-mRNA-1;Parent=Msa0867650;Name=Msa0867650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201 |
| chr6_1 | exon | 717870 | 718475 | 717870 | ID=Msa0867650-mRNA-1:exon:215;Parent=Msa0867650-mRNA-1 |
| chr6_1 | CDS | 717870 | 718475 | 717870 | ID=Msa0867650-mRNA-1:cds;Parent=Msa0867650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
