AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1398700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1398700 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398700 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398700 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398700 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398700 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1398700 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1398700 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1398700 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0022279:-8362 Msa1398700:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0022279:+8382 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0022279:-8393 Msa1398700:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0022279:-8693 Msa1398700:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0022279:-8637 Msa1398700:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0022279:-8334 Msa1398700:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0022279:+8422 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0022279:-8836 Msa1398700:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0022279:+8513 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0022279:+8387 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0022279:+8568 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0022279:-8361 Msa1398700:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0022279:-8357 Msa1398700:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0022279:+8813 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0022279:+8408 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0022279:-8672 Msa1398700:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0022279:+8569 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0022279:+8834 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0022279:+8421 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0022279:-8493 Msa1398700:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0022279:+8495 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0022279:+8653 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0022279:-8825 Msa1398700:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0022279:-8492 Msa1398700:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0022279:+8576 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0022279:+8403 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0022279:+8425 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0022279:+8423 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0022279:-8893 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0022279:-8894 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0022279:-8681 Msa1398700:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0022279:+8553 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0022279:+8424 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0022279:8831-8850 Msa1398700:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0022279:8832-8851 Msa1398700:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0022279:8859-8878 Msa1398700:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0022279:8458-8477 Msa1398700:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0022279:8543-8562 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0022279:8578-8597 Msa1398700:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0022279:8809-8828 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0022279:8814-8833 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0022279:8836-8855 Msa1398700:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0022279:8328-8347 Msa1398700:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0022279:8329-8348 Msa1398700:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0022279:8556-8575 Msa1398700:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0022279:8577-8596 Msa1398700:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0022279:8643-8662 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0022279:8641-8660 Msa1398700:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0022279:8701-8720 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0022279:8700-8719 Msa1398700:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0022279:8701-8720 Msa1398700:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0022279:8800-8819 Msa1398700:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0022279:8326-8345 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0022279:8330-8349 Msa1398700:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0022279:8357-8376 Msa1398700:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0022279:8583-8602 Msa1398700:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0022279:8627-8646 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0022279:8628-8647 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0022279:8331-8350 Msa1398700:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0022279:8409-8428 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0022279:8500-8519 Msa1398700:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0022279:8620-8639 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0022279:8683-8702 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0022279:8775-8794 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0022279:8788-8807 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0022279:8793-8812 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0022279:8368-8387 Msa1398700:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0022279:8383-8402 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0022279:8521-8540 Msa1398700:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0022279:8774-8793 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0022279:8362-8381 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0022279:8512-8531 Msa1398700:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0022279:8773-8792 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0022279:8771-8790 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0022279:8772-8791 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0022279:8341-8360 Msa1398700:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0022279 gene 8305 8910 8305 ID=Msa1398700;Name=Msa1398700
tig0022279 mRNA 8305 8910 8305 ID=Msa1398700-mRNA-1;Parent=Msa1398700;Name=Msa1398700-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0022279 exon 8305 8910 8305 ID=Msa1398700-mRNA-1:exon:16775;Parent=Msa1398700-mRNA-1
tig0022279 CDS 8305 8910 8305 ID=Msa1398700-mRNA-1:cds;Parent=Msa1398700-mRNA-1
Gene Sequence

>Msa1398700

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1398700

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*