AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1368300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368300 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368300 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368300 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368300 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368300 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1368300 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1368300 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1368300 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0018631:-162753 Msa1368300:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0018631:+162773 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0018631:-162784 Msa1368300:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0018631:-163084 Msa1368300:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0018631:-163028 Msa1368300:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0018631:-162725 Msa1368300:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0018631:+162813 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0018631:-163227 Msa1368300:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0018631:+162904 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0018631:+162778 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0018631:+162959 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0018631:-162752 Msa1368300:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0018631:-162748 Msa1368300:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0018631:+163204 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0018631:+162799 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0018631:-163063 Msa1368300:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0018631:+162960 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0018631:+163225 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0018631:+162812 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0018631:-162884 Msa1368300:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0018631:+162886 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0018631:+163044 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0018631:-163216 Msa1368300:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0018631:-162883 Msa1368300:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0018631:+162967 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0018631:+162794 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0018631:+162816 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0018631:+162814 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0018631:-163284 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0018631:-163285 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0018631:-163072 Msa1368300:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0018631:+162944 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0018631:+162815 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0018631:163222-163241 Msa1368300:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0018631:163223-163242 Msa1368300:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0018631:163250-163269 Msa1368300:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0018631:162849-162868 Msa1368300:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0018631:162934-162953 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0018631:162969-162988 Msa1368300:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0018631:163200-163219 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0018631:163205-163224 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0018631:163227-163246 Msa1368300:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0018631:162719-162738 Msa1368300:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0018631:162720-162739 Msa1368300:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0018631:162947-162966 Msa1368300:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0018631:162968-162987 Msa1368300:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0018631:163034-163053 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0018631:163032-163051 Msa1368300:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0018631:163092-163111 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0018631:163091-163110 Msa1368300:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0018631:163092-163111 Msa1368300:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0018631:163191-163210 Msa1368300:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0018631:162717-162736 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0018631:162721-162740 Msa1368300:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0018631:162748-162767 Msa1368300:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0018631:162974-162993 Msa1368300:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0018631:163018-163037 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0018631:163019-163038 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0018631:162722-162741 Msa1368300:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0018631:162800-162819 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0018631:162891-162910 Msa1368300:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0018631:163011-163030 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0018631:163074-163093 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0018631:163166-163185 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0018631:163179-163198 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0018631:163184-163203 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0018631:162759-162778 Msa1368300:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0018631:162774-162793 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0018631:162912-162931 Msa1368300:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0018631:163165-163184 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0018631:162753-162772 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0018631:162903-162922 Msa1368300:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0018631:163164-163183 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0018631:163162-163181 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0018631:163163-163182 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0018631:162732-162751 Msa1368300:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018631 gene 162696 163301 162696 ID=Msa1368300;Name=Msa1368300
tig0018631 mRNA 162696 163301 162696 ID=Msa1368300-mRNA-1;Parent=Msa1368300;Name=Msa1368300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0018631 exon 162696 163301 162696 ID=Msa1368300-mRNA-1:exon:402;Parent=Msa1368300-mRNA-1
tig0018631 CDS 162696 163301 162696 ID=Msa1368300-mRNA-1:cds;Parent=Msa1368300-mRNA-1
Gene Sequence

>Msa1368300

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1368300

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*