AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1354390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354390 MtrunA17_Chr7g0229301 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354390 MtrunA17_Chr6g0454441 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354390 MtrunA17_Chr4g0016451 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354390 MtrunA17_Chr6g0466281 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354390 MtrunA17_CPg0493311 97.512 201 5 0 1 201 1 201 1.37e-143 398
Msa1354390 MtrunA17_Chr7g0229661 84.080 201 13 1 1 201 1 182 1.89e-115 327
Msa1354390 MtrunA17_Chr4g0015291 97.436 156 4 0 1 156 1 156 3.32e-108 307
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1354390 ATCG00380.1 88.060 201 24 0 1 201 1 201 2.12e-126 355

Find 33 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 tig0014543:-224446 Msa1354390:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 tig0014543:+224466 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 tig0014543:-224477 Msa1354390:CDS
CTTAAATACGTTCGTATCGC+TGG 0.342652 tig0014543:-224777 Msa1354390:CDS
ACAATTACTTGAAATGCGTT+TGG 0.359489 tig0014543:-224721 Msa1354390:CDS
TTTGAAAATTAATGAATTGC+TGG 0.365999 tig0014543:-224418 Msa1354390:CDS
ATGGATTGGCAATTCTTCGC+GGG 0.450528 tig0014543:+224506 None:intergenic
TACCAGGACTAACTAGTAAA+CGG 0.463029 tig0014543:-224920 Msa1354390:CDS
TGAGGTTTGCAACGATAACT+TGG 0.468099 tig0014543:+224597 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 tig0014543:+224471 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 tig0014543:+224652 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 tig0014543:-224445 Msa1354390:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 tig0014543:-224441 Msa1354390:CDS
CTAAGTTCGCTTCCAACTGT+AGG 0.508663 tig0014543:+224897 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 tig0014543:+224492 None:intergenic
GGAAAAGCGAAAGGTTCAAC+CGG 0.516182 tig0014543:-224756 Msa1354390:CDS
TCTGTGGTTAACTAATTGAC+GGG 0.521261 tig0014543:+224653 None:intergenic
GGCCGTTTACTAGTTAGTCC+TGG 0.527000 tig0014543:+224918 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 tig0014543:+224505 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 tig0014543:-224577 Msa1354390:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 tig0014543:+224579 None:intergenic
TAATTGTAGTAAAACTTGAC+CGG 0.568250 tig0014543:+224737 None:intergenic
ACTAGTAAACGGCCTACAGT+TGG 0.568372 tig0014543:-224909 Msa1354390:CDS
CAAGATATTATTACAGCGAA+GGG 0.569002 tig0014543:-224576 Msa1354390:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 tig0014543:+224660 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 tig0014543:+224487 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 tig0014543:+224509 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 tig0014543:+224507 None:intergenic
TGTTTATGTCACGTTACAGA+GGG 0.632465 tig0014543:-224977 None:intergenic
GTGTTTATGTCACGTTACAG+AGG 0.633128 tig0014543:-224978 None:intergenic
CGTATCGCTGGAAAAGCGAA+AGG 0.633501 tig0014543:-224765 Msa1354390:CDS
CATTAACTAAAACATGTCTG+TGG 0.663264 tig0014543:+224637 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 tig0014543:+224508 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAATAGATAGTAAA+TGG - tig0014543:224915-224934 Msa1354390:CDS 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - tig0014543:224916-224935 Msa1354390:CDS 15.0%
!! TTTGAAAATTAATGAATTGC+TGG - tig0014543:224943-224962 Msa1354390:CDS 20.0%
!! CAAAAATTGCGTTTTCATTA+TGG - tig0014543:224542-224561 Msa1354390:CDS 25.0%
! TAATTGTAGTAAAACTTGAC+CGG + tig0014543:224627-224646 None:intergenic 25.0%
!!! GATAACATACTTTTTCGATT+GGG - tig0014543:224662-224681 Msa1354390:CDS 25.0%
! ACTAATCCTTTATATTGGAA+TGG + tig0014543:224893-224912 None:intergenic 25.0%
! GATTGACTAATCCTTTATAT+TGG + tig0014543:224898-224917 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - tig0014543:224920-224939 Msa1354390:CDS 25.0%
TTTCAAAAAAATACGTCGTC+TGG - tig0014543:224412-224431 Msa1354390:CDS 30.0%
TTCAAAAAAATACGTCGTCT+GGG - tig0014543:224413-224432 Msa1354390:CDS 30.0%
ACAATTACTTGAAATGCGTT+TGG - tig0014543:224640-224659 Msa1354390:CDS 30.0%
!!! GGATAACATACTTTTTCGAT+TGG - tig0014543:224661-224680 Msa1354390:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + tig0014543:224727-224746 None:intergenic 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - tig0014543:224725-224744 Msa1354390:CDS 30.0%
TTCGCTGTAATAATATCTTG+AGG + tig0014543:224785-224804 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - tig0014543:224784-224803 Msa1354390:CDS 30.0%
CAAGATATTATTACAGCGAA+GGG - tig0014543:224785-224804 Msa1354390:CDS 30.0%
CTTGATCCATTCCAATATAA+AGG - tig0014543:224884-224903 Msa1354390:CDS 30.0%
!!! CGACGTATTTTTTTGAAACG+AGG + tig0014543:224410-224429 None:intergenic 35.0%
TCAAAAAAATACGTCGTCTG+GGG - tig0014543:224414-224433 Msa1354390:CDS 35.0%
TACCAGGACTAACTAGTAAA+CGG - tig0014543:224441-224460 Msa1354390:CDS 35.0%
!!! CATACTTTTTCGATTGGGTA+TGG - tig0014543:224667-224686 Msa1354390:CDS 35.0%
TCTGTGGTTAACTAATTGAC+GGG + tig0014543:224711-224730 None:intergenic 35.0%
GTCTGTGGTTAACTAATTGA+CGG + tig0014543:224712-224731 None:intergenic 35.0%
CAAAAAAATACGTCGTCTGG+GGG - tig0014543:224415-224434 Msa1354390:CDS 40.0%
!!! ATTTTTTACTCGAGCGCGAT+TGG + tig0014543:224493-224512 None:intergenic 40.0%
CTTAAATACGTTCGTATCGC+TGG - tig0014543:224584-224603 Msa1354390:CDS 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + tig0014543:224704-224723 None:intergenic 40.0%
TGAGGTTTGCAACGATAACT+TGG + tig0014543:224767-224786 None:intergenic 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + tig0014543:224859-224878 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + tig0014543:224872-224891 None:intergenic 40.0%
GGAATGGATCAAGAGTCAAA+TGG + tig0014543:224877-224896 None:intergenic 40.0%
ACTAGTAAACGGCCTACAGT+TGG - tig0014543:224452-224471 Msa1354390:CDS 45.0%
CTAAGTTCGCTTCCAACTGT+AGG + tig0014543:224467-224486 None:intergenic 45.0%
GGAAAAGCGAAAGGTTCAAC+CGG - tig0014543:224605-224624 Msa1354390:CDS 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + tig0014543:224858-224877 None:intergenic 45.0%
GGCCGTTTACTAGTTAGTCC+TGG + tig0014543:224446-224465 None:intergenic 50.0%
CGTATCGCTGGAAAAGCGAA+AGG - tig0014543:224596-224615 Msa1354390:CDS 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + tig0014543:224857-224876 None:intergenic 50.0%
GATTGGCAATTCTTCGCGGG+GGG + tig0014543:224855-224874 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + tig0014543:224856-224875 None:intergenic 55.0%
CGTCGTCTGGGGGCTTTACC+AGG - tig0014543:224425-224444 Msa1354390:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0014543 gene 224389 224994 224389 ID=Msa1354390;Name=Msa1354390
tig0014543 mRNA 224389 224994 224389 ID=Msa1354390-mRNA-1;Parent=Msa1354390;Name=Msa1354390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201
tig0014543 exon 224389 224994 224389 ID=Msa1354390-mRNA-1:exon:9113;Parent=Msa1354390-mRNA-1
tig0014543 CDS 224389 224994 224389 ID=Msa1354390-mRNA-1:cds;Parent=Msa1354390-mRNA-1
Gene Sequence

>Msa1354390

ATGTCACGTTACAGAGGGCCTCGTTTCAAAAAAATACGTCGTCTGGGGGCTTTACCAGGACTAACTAGTAAACGGCCTACAGTTGGAAGCGAACTTAGAAACCAATCGCGCTCGAGTAAAAAATCTCAATATCGTATTCGTTTAGAAGAAAAACAAAAATTGCGTTTTCATTATGGTCTTACAGAACGACAATTGCTTAAATACGTTCGTATCGCTGGAAAAGCGAAAGGTTCAACCGGTCAAGTTTTACTACAATTACTTGAAATGCGTTTGGATAACATACTTTTTCGATTGGGTATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGGTTGTAGAATATTATTCTCGTCAGACTTAA

Protein sequence

>Msa1354390

MSRYRGPRFKKIRRLGALPGLTSKRPTVGSELRNQSRSSKKSQYRIRLEEKQKLRFHYGLTERQLLKYVRIAGKAKGSTGQVLLQLLEMRLDNILFRLGMASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLVVEYYSRQT*