AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0541410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541410 MtrunA17_Chr4g0018731 78.846 104 16 2 24 123 71 172 5.24e-27 99.0
Msa0541410 MtrunA17_Chr1g0168301 81.897 116 17 1 24 135 210 325 3.64e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAGTGTTTAAGACTAAC+AGG 0.286414 4_1:-25284657 Msa0541410:CDS
ATCATCAACTGACAGTGATT+CGG 0.382705 4_1:-25284419 Msa0541410:CDS
AAAGGAGGAGGAGGGAGCAC+AGG 0.386258 4_1:-25284606 Msa0541410:CDS
TCATCAACTGACAGTGATTC+GGG 0.386672 4_1:-25284418 Msa0541410:CDS
GATTCGGGTGATGGATATAA+TGG 0.404972 4_1:-25284403 Msa0541410:CDS
ACCATGGAGATGATGATTAG+AGG 0.412561 4_1:-25284330 None:intergenic
TGGATATAATGGTTCAAGTA+AGG 0.433386 4_1:-25284392 Msa0541410:CDS
CTATATTCATCACTATCATC+TGG 0.440092 4_1:+25284562 None:intergenic
GCTAGTGATTCAGATGGTTC+CGG 0.468678 4_1:-25284631 Msa0541410:CDS
ACTGACAGTGATTCGGGTGA+TGG 0.486358 4_1:-25284412 Msa0541410:CDS
CTCCCTCCTCCTCCTTTCAC+CGG 0.487250 4_1:+25284612 None:intergenic
GTAGGAAAGAGAAGAGGAGA+AGG 0.494224 4_1:-25284522 Msa0541410:CDS
ACAAAGATGCTGACCGAAAG+AGG 0.498522 4_1:-25284703 Msa0541410:CDS
ATTCAGATGGTTCCGGTGAA+AGG 0.502557 4_1:-25284624 Msa0541410:CDS
TCCTCTAATCATCATCTCCA+TGG 0.523954 4_1:+25284329 None:intergenic
GTTCCGGTGAAAGGAGGAGG+AGG 0.525308 4_1:-25284615 Msa0541410:CDS
ATGAATATAGACACAAGAAA+AGG 0.532593 4_1:-25284549 Msa0541410:CDS
GGATATAATGGTTCAAGTAA+GGG 0.549938 4_1:-25284391 Msa0541410:CDS
ATGGTTCCGGTGAAAGGAGG+AGG 0.558346 4_1:-25284618 Msa0541410:CDS
TAATGGTTCAAGTAAGGGTA+GGG 0.560844 4_1:-25284386 Msa0541410:CDS
GACACAAGAAAAGGAAGAGT+AGG 0.567421 4_1:-25284540 Msa0541410:CDS
TTCTGATTCTGAAACATCTG+AGG 0.573778 4_1:-25284482 Msa0541410:CDS
AGAGAGGTAGAGAAAAGAGA+AGG 0.579127 4_1:-25284676 Msa0541410:CDS
ATAATGGTTCAAGTAAGGGT+AGG 0.582111 4_1:-25284387 Msa0541410:CDS
GGAAGAGTAGGAAAGAGAAG+AGG 0.590104 4_1:-25284528 Msa0541410:CDS
GGGAGCACAGGAGAAGCATG+AGG 0.590249 4_1:-25284594 Msa0541410:CDS
AAGAGGAGGAGACGCCACCA+TGG 0.594257 4_1:-25284346 Msa0541410:CDS
CAGATGGTTCCGGTGAAAGG+AGG 0.617536 4_1:-25284621 Msa0541410:CDS
CATCTGAGGATTCTACTTCG+CGG 0.617958 4_1:-25284468 Msa0541410:CDS
AGGGAAGCTAGTGATTCAGA+TGG 0.649064 4_1:-25284637 Msa0541410:CDS
GGAGCACAGGAGAAGCATGA+GGG 0.656858 4_1:-25284593 Msa0541410:CDS
TTCCGGTGAAAGGAGGAGGA+GGG 0.666380 4_1:-25284614 Msa0541410:CDS
AGGAGTGTTTAAGACTAACA+GGG 0.683554 4_1:-25284656 Msa0541410:CDS
TCTAATCATCATCTCCATGG+TGG 0.691101 4_1:+25284332 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCAAAAAATCAGAAAAG+AGG - chr4_1:25284688-25284707 Msa0541410:CDS 20.0%
! ATGAATATAGACACAAGAAA+AGG - chr4_1:25284502-25284521 Msa0541410:CDS 25.0%
CTATATTCATCACTATCATC+TGG + chr4_1:25284492-25284511 None:intergenic 30.0%
TGGATATAATGGTTCAAGTA+AGG - chr4_1:25284659-25284678 Msa0541410:CDS 30.0%
GGATATAATGGTTCAAGTAA+GGG - chr4_1:25284660-25284679 Msa0541410:CDS 30.0%
TCAAAAAATCAGAAAAGAGG+AGG - chr4_1:25284691-25284710 Msa0541410:CDS 30.0%
! TACCTCTCTTTTTCCTCTTT+CGG + chr4_1:25284364-25284383 None:intergenic 35.0%
!! AAGGAGTGTTTAAGACTAAC+AGG - chr4_1:25284394-25284413 Msa0541410:CDS 35.0%
!! AGGAGTGTTTAAGACTAACA+GGG - chr4_1:25284395-25284414 Msa0541410:CDS 35.0%
!! TTCTGATTCTGAAACATCTG+AGG - chr4_1:25284569-25284588 Msa0541410:CDS 35.0%
ATCATCAACTGACAGTGATT+CGG - chr4_1:25284632-25284651 Msa0541410:CDS 35.0%
ATAATGGTTCAAGTAAGGGT+AGG - chr4_1:25284664-25284683 Msa0541410:CDS 35.0%
TAATGGTTCAAGTAAGGGTA+GGG - chr4_1:25284665-25284684 Msa0541410:CDS 35.0%
AGAGAGGTAGAGAAAAGAGA+AGG - chr4_1:25284375-25284394 Msa0541410:CDS 40.0%
GACACAAGAAAAGGAAGAGT+AGG - chr4_1:25284511-25284530 Msa0541410:CDS 40.0%
TCATCAACTGACAGTGATTC+GGG - chr4_1:25284633-25284652 Msa0541410:CDS 40.0%
!! GATTCGGGTGATGGATATAA+TGG - chr4_1:25284648-25284667 Msa0541410:CDS 40.0%
ACAAAGATGCTGACCGAAAG+AGG - chr4_1:25284348-25284367 Msa0541410:CDS 45.0%
GACCGAAAGAGGAAAAAGAG+AGG - chr4_1:25284359-25284378 Msa0541410:CDS 45.0%
AGGGAAGCTAGTGATTCAGA+TGG - chr4_1:25284414-25284433 Msa0541410:CDS 45.0%
GCTAGTGATTCAGATGGTTC+CGG - chr4_1:25284420-25284439 Msa0541410:CDS 45.0%
ATTCAGATGGTTCCGGTGAA+AGG - chr4_1:25284427-25284446 Msa0541410:CDS 45.0%
GGAAGAGTAGGAAAGAGAAG+AGG - chr4_1:25284523-25284542 Msa0541410:CDS 45.0%
GTAGGAAAGAGAAGAGGAGA+AGG - chr4_1:25284529-25284548 Msa0541410:CDS 45.0%
CATCTGAGGATTCTACTTCG+CGG - chr4_1:25284583-25284602 Msa0541410:CDS 45.0%
! ACTGACAGTGATTCGGGTGA+TGG - chr4_1:25284639-25284658 Msa0541410:CDS 50.0%
CAGATGGTTCCGGTGAAAGG+AGG - chr4_1:25284430-25284449 Msa0541410:CDS 55.0%
ATGGTTCCGGTGAAAGGAGG+AGG - chr4_1:25284433-25284452 Msa0541410:CDS 55.0%
TTCCGGTGAAAGGAGGAGGA+GGG - chr4_1:25284437-25284456 Msa0541410:CDS 55.0%
! GGAGCACAGGAGAAGCATGA+GGG - chr4_1:25284458-25284477 Msa0541410:CDS 55.0%
GTTCCGGTGAAAGGAGGAGG+AGG - chr4_1:25284436-25284455 Msa0541410:CDS 60.0%
CTCCCTCCTCCTCCTTTCAC+CGG + chr4_1:25284442-25284461 None:intergenic 60.0%
! AAAGGAGGAGGAGGGAGCAC+AGG - chr4_1:25284445-25284464 Msa0541410:CDS 60.0%
! GGGAGCACAGGAGAAGCATG+AGG - chr4_1:25284457-25284476 Msa0541410:CDS 60.0%
AAGAGGAGGAGACGCCACCA+TGG - chr4_1:25284705-25284724 Msa0541410:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 25284333 25284740 25284333 ID=Msa0541410;Name=Msa0541410
chr4_1 mRNA 25284333 25284740 25284333 ID=Msa0541410-mRNA-1;Parent=Msa0541410;Name=Msa0541410-mRNA-1;_AED=0.39;_eAED=0.56;_QI=0|-1|0|1|-1|1|1|0|135
chr4_1 exon 25284333 25284740 25284333 ID=Msa0541410-mRNA-1:exon:6795;Parent=Msa0541410-mRNA-1
chr4_1 CDS 25284333 25284740 25284333 ID=Msa0541410-mRNA-1:cds;Parent=Msa0541410-mRNA-1
Gene Sequence

>Msa0541410

ATGATGACGAGTCAGACAAAGATGCTGACCGAAAGAGGAAAAAGAGAGGTAGAGAAAAGAGAAGGAGTGTTTAAGACTAACAGGGAAGCTAGTGATTCAGATGGTTCCGGTGAAAGGAGGAGGAGGGAGCACAGGAGAAGCATGAGGGACGAGTCTCCAGATGATAGTGATGAATATAGACACAAGAAAAGGAAGAGTAGGAAAGAGAAGAGGAGAAGGAGAAGTCGTCTTTCAGATTCTGATTCTGAAACATCTGAGGATTCTACTTCGCGGCACAAGCGAAATAGCAAGAGAACTTCATCATCAACTGACAGTGATTCGGGTGATGGATATAATGGTTCAAGTAAGGGTAGGGATATCAAAAAATCAGAAAAGAGGAGGAGACGCCACCATGGAGATGATGATTAG

Protein sequence

>Msa0541410

MMTSQTKMLTERGKREVEKREGVFKTNREASDSDGSGERRRREHRRSMRDESPDDSDEYRHKKRKSRKEKRRRRSRLSDSDSETSEDSTSRHKRNSKRTSSSTDSDSGDGYNGSSKGRDIKKSEKRRRRHHGDDD*