AlfalfaGEDB | Msa1431850

M. sativa cultivar zhongmu-4 / Msa1431850

KEGG: -
GO: GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009639 GO:0009653 GO:0009826 GO:0009987 GO:0010114 GO:0010218 GO:0010467 GO:0016043 GO:0016049 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0071704 GO:0071840 GO:0090304 GO:0090698 GO:0097659 GO:1901360 GO:1901362 GO:1901576
PFAM: PF04852.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431850	XP_013450572.1	97.647	170	3	1	1	169	1	170	3.41e-120	346

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431850	sp\|Q9FGH2\|LSH5_ARATH	70.423	142	36	4	26	163	18	157	1.68e-66	203

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431850	I3T2Z0	97.647	170	3	1	1	169	1	170	1.63e-120	346

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002930	Msa1431850	0.808174	3.526392e-50	-8.615850e-47
Msa0006980	Msa1431850	0.868397	6.732506e-66	-8.615850e-47
Msa0015710	Msa1431850	0.876551	1.275907e-68	-8.615850e-47
Msa0016120	Msa1431850	0.855944	4.490587e-62	-8.615850e-47
Msa0019600	Msa1431850	0.863626	2.176861e-64	-8.615850e-47
Msa0025030	Msa1431850	0.801007	1.103019e-48	-8.615850e-47
Msa0050040	Msa1431850	0.826511	2.606667e-54	-8.615850e-47
Msa0055450	Msa1431850	0.845690	3.469821e-59	-8.615850e-47
Msa0074800	Msa1431850	0.818274	2.134887e-52	-8.615850e-47
Msa0080990	Msa1431850	0.842065	3.247060e-58	-8.615850e-47
Msa0102990	Msa1431850	0.825279	5.110443e-54	-8.615850e-47
Msa0109880	Msa1431850	0.877788	4.742348e-69	-8.615850e-47
Msa0111750	Msa1431850	0.831412	1.694332e-55	-8.615850e-47
Msa1356990	Msa1431850	0.836749	7.796136e-57	-8.615850e-47
Msa1374550	Msa1431850	0.806874	6.655984e-50	-8.615850e-47
Msa1378870	Msa1431850	0.838858	2.240234e-57	-8.615850e-47
Msa1379970	Msa1431850	0.895615	8.100002e-76	-8.615850e-47
Msa1388770	Msa1431850	0.812495	4.119889e-51	-8.615850e-47
Msa1394890	Msa1431850	0.857619	1.443240e-62	-8.615850e-47
Msa1406780	Msa1431850	0.993804	2.409325e-202	-8.615850e-47
Msa1420060	Msa1431850	0.822019	2.962359e-53	-8.615850e-47
Msa1421550	Msa1431850	0.889785	1.774847e-73	-8.615850e-47
Msa1423460	Msa1431850	0.861151	1.254505e-63	-8.615850e-47
Msa1431280	Msa1431850	0.802214	6.238065e-49	-8.615850e-47
Msa1431850	Msa1435480	0.832641	8.417528e-56	-8.615850e-47
Msa1431850	Msa1437710	0.818099	2.339563e-52	-8.615850e-47
Msa1431850	Msa1449940	0.847048	1.479105e-59	-8.615850e-47
Msa1431850	Msa1455320	0.831648	1.481491e-55	-8.615850e-47
Msa1431850	Msa1455590	0.806225	9.120600e-50	-8.615850e-47
Msa1431850	Msa1456730	0.804534	2.063277e-49	-8.615850e-47
Msa1431850	Msa1458910	0.803354	3.628489e-49	-8.615850e-47
Msa1431850	Msa1459530	0.840386	8.972051e-58	-8.615850e-47
Msa1431850	Msa1461060	0.870196	1.752175e-66	-8.615850e-47
Msa1431850	Msa1462530	0.826720	2.322937e-54	-8.615850e-47
Msa0641250	Msa1431850	0.851119	1.092021e-60	-8.615850e-47
Msa0641360	Msa1431850	0.825299	5.055182e-54	-8.615850e-47
Msa0669840	Msa1431850	0.915264	7.237986e-85	-8.615850e-47
Msa0679830	Msa1431850	0.805340	1.399402e-49	-8.615850e-47
Msa0680150	Msa1431850	0.803959	2.718165e-49	-8.615850e-47
Msa0707220	Msa1431850	0.810953	8.918966e-51	-8.615850e-47
Msa0709450	Msa1431850	0.826559	2.538214e-54	-8.615850e-47
Msa0711320	Msa1431850	0.829429	5.174940e-55	-8.615850e-47
Msa0725860	Msa1431850	0.839645	1.400056e-57	-8.615850e-47
Msa0727930	Msa1431850	0.802149	6.434442e-49	-8.615850e-47
Msa0739580	Msa1431850	0.958916	7.166599e-117	-8.615850e-47
Msa0748000	Msa1431850	0.841417	4.812909e-58	-8.615850e-47
Msa0751890	Msa1431850	0.831839	1.329264e-55	-8.615850e-47
Msa0752840	Msa1431850	0.810207	1.292709e-50	-8.615850e-47
Msa0764820	Msa1431850	0.813873	2.053265e-51	-8.615850e-47
Msa0770290	Msa1431850	0.836932	7.003844e-57	-8.615850e-47
Msa0782230	Msa1431850	0.826094	3.275867e-54	-8.615850e-47
Msa0782360	Msa1431850	0.930430	1.656694e-93	-8.615850e-47
Msa0819890	Msa1431850	0.876910	9.589966e-69	-8.615850e-47
Msa0823400	Msa1431850	0.826576	2.515175e-54	-8.615850e-47
Msa0828830	Msa1431850	0.881545	2.195055e-70	-8.615850e-47
Msa0831940	Msa1431850	0.834518	2.862295e-56	-8.615850e-47
Msa0124130	Msa1431850	0.898349	5.789620e-77	-8.615850e-47
Msa0125170	Msa1431850	0.813138	2.978717e-51	-8.615850e-47
Msa0141120	Msa1431850	0.852702	3.880644e-61	-8.615850e-47
Msa0147600	Msa1431850	0.811757	5.967994e-51	-8.615850e-47
Msa0149140	Msa1431850	0.887193	1.769365e-72	-8.615850e-47
Msa0152500	Msa1431850	0.824892	6.309069e-54	-8.615850e-47
Msa0156250	Msa1431850	0.888412	6.039649e-73	-8.615850e-47
Msa0169910	Msa1431850	0.805846	1.096032e-49	-8.615850e-47
Msa0185490	Msa1431850	0.858745	6.670910e-63	-8.615850e-47
Msa0197100	Msa1431850	0.883171	5.624071e-71	-8.615850e-47
Msa0203030	Msa1431850	0.831312	1.792724e-55	-8.615850e-47
Msa0212080	Msa1431850	0.829727	4.379427e-55	-8.615850e-47
Msa0212150	Msa1431850	0.910487	1.773410e-82	-8.615850e-47
Msa0215640	Msa1431850	0.843979	1.004044e-58	-8.615850e-47
Msa0217230	Msa1431850	0.807679	4.492402e-50	-8.615850e-47
Msa0219190	Msa1431850	0.811616	6.405342e-51	-8.615850e-47
Msa0221290	Msa1431850	0.835135	2.001756e-56	-8.615850e-47
Msa1228190	Msa1431850	0.888019	8.555813e-73	-8.615850e-47
Msa1232730	Msa1431850	0.803824	2.899091e-49	-8.615850e-47
Msa1233560	Msa1431850	0.843292	1.532270e-58	-8.615850e-47
Msa1248040	Msa1431850	0.807385	5.188336e-50	-8.615850e-47
Msa1271260	Msa1431850	0.861845	7.707040e-64	-8.615850e-47
Msa1277400	Msa1431850	0.824555	7.577287e-54	-8.615850e-47
Msa1285900	Msa1431850	0.806943	6.433248e-50	-8.615850e-47
Msa1294290	Msa1431850	0.884827	1.374800e-71	-8.615850e-47
Msa1305310	Msa1431850	0.817980	2.489041e-52	-8.615850e-47
Msa1305510	Msa1431850	0.878501	2.668966e-69	-8.615850e-47
Msa1307150	Msa1431850	0.819319	1.236131e-52	-8.615850e-47
Msa1307190	Msa1431850	0.837986	3.758963e-57	-8.615850e-47
Msa1313970	Msa1431850	0.821078	4.889095e-53	-8.615850e-47
Msa1316540	Msa1431850	0.819134	1.361837e-52	-8.615850e-47
Msa1326630	Msa1431850	0.820609	6.266716e-53	-8.615850e-47
Msa1336620	Msa1431850	0.802144	6.448930e-49	-8.615850e-47
Msa1336780	Msa1431850	0.801253	9.824683e-49	-8.615850e-47
Msa1339580	Msa1431850	0.820180	7.860035e-53	-8.615850e-47
Msa0373960	Msa1431850	0.836127	1.122888e-56	-8.615850e-47
Msa0375680	Msa1431850	0.840917	6.514246e-58	-8.615850e-47
Msa0375890	Msa1431850	0.801320	9.518733e-49	-8.615850e-47
Msa0384910	Msa1431850	0.859330	4.456401e-63	-8.615850e-47
Msa0385390	Msa1431850	0.805917	1.058971e-49	-8.615850e-47
Msa0392420	Msa1431850	0.843941	1.027727e-58	-8.615850e-47
Msa0392460	Msa1431850	0.839392	1.628655e-57	-8.615850e-47
Msa0392490	Msa1431850	0.817745	2.811522e-52	-8.615850e-47
Msa0406000	Msa1431850	0.934341	4.693240e-96	-8.615850e-47
Msa0415320	Msa1431850	0.819128	1.366414e-52	-8.615850e-47
Msa0417440	Msa1431850	0.809358	1.968347e-50	-8.615850e-47
Msa0424330	Msa1431850	0.878175	3.474544e-69	-8.615850e-47
Msa0424340	Msa1431850	0.879981	7.993142e-70	-8.615850e-47
Msa0424350	Msa1431850	0.877986	4.046167e-69	-8.615850e-47
Msa0441590	Msa1431850	0.817764	2.784891e-52	-8.615850e-47
Msa0451400	Msa1431850	0.933714	1.231651e-95	-8.615850e-47
Msa0469260	Msa1431850	0.814356	1.606670e-51	-8.615850e-47
Msa0471240	Msa1431850	0.827406	1.592655e-54	-8.615850e-47
Msa0477460	Msa1431850	0.801893	7.262570e-49	-8.615850e-47
Msa0479540	Msa1431850	0.820410	6.959887e-53	-8.615850e-47
Msa0996270	Msa1431850	0.831821	1.343035e-55	-8.615850e-47
Msa0999300	Msa1431850	0.881635	2.037421e-70	-8.615850e-47
Msa0999320	Msa1431850	0.845859	3.121678e-59	-8.615850e-47
Msa0999340	Msa1431850	0.800384	1.478297e-48	-8.615850e-47
Msa0999350	Msa1431850	0.838388	2.962625e-57	-8.615850e-47
Msa0999360	Msa1431850	0.827884	1.222683e-54	-8.615850e-47
Msa0999380	Msa1431850	0.800570	1.354748e-48	-8.615850e-47
Msa0999400	Msa1431850	0.828393	9.215347e-55	-8.615850e-47
Msa0999410	Msa1431850	0.830003	3.750842e-55	-8.615850e-47
Msa1001870	Msa1431850	0.846306	2.359801e-59	-8.615850e-47
Msa1001890	Msa1431850	0.846405	2.216812e-59	-8.615850e-47
Msa1010840	Msa1431850	0.809728	1.638947e-50	-8.615850e-47
Msa1016600	Msa1431850	0.814884	1.227378e-51	-8.615850e-47
Msa1034680	Msa1431850	0.815678	8.172837e-52	-8.615850e-47
Msa1037770	Msa1431850	0.874920	4.632075e-68	-8.615850e-47
Msa1037890	Msa1431850	0.830462	2.897072e-55	-8.615850e-47
Msa1037900	Msa1431850	0.851617	7.897200e-61	-8.615850e-47
Msa1037910	Msa1431850	0.845703	3.442840e-59	-8.615850e-47
Msa1041530	Msa1431850	0.823630	1.248810e-53	-8.615850e-47
Msa1047990	Msa1431850	0.809004	2.344081e-50	-8.615850e-47
Msa1052360	Msa1431850	0.813483	2.501501e-51	-8.615850e-47
Msa1081570	Msa1431850	0.839673	1.376823e-57	-8.615850e-47
Msa1084520	Msa1431850	0.855048	8.190153e-62	-8.615850e-47
Msa1092020	Msa1431850	0.825718	4.023027e-54	-8.615850e-47
Msa1096210	Msa1431850	0.828559	8.403456e-55	-8.615850e-47
Msa0239030	Msa1431850	0.891954	2.476533e-74	-8.615850e-47
Msa0252630	Msa1431850	0.843374	1.457303e-58	-8.615850e-47
Msa0252770	Msa1431850	0.914980	1.013099e-84	-8.615850e-47
Msa0266930	Msa1431850	0.801752	7.763266e-49	-8.615850e-47
Msa0276080	Msa1431850	0.804413	2.185843e-49	-8.615850e-47
Msa0276740	Msa1431850	0.870230	1.706737e-66	-8.615850e-47
Msa0289920	Msa1431850	0.904397	1.280821e-79	-8.615850e-47
Msa0291330	Msa1431850	0.811367	7.252869e-51	-8.615850e-47
Msa0298240	Msa1431850	0.808415	3.132819e-50	-8.615850e-47
Msa0306120	Msa1431850	0.915910	3.354458e-85	-8.615850e-47
Msa0316680	Msa1431850	0.880078	7.379568e-70	-8.615850e-47
Msa0322830	Msa1431850	0.820996	5.105858e-53	-8.615850e-47
Msa0322840	Msa1431850	0.837248	5.812525e-57	-8.615850e-47
Msa0322860	Msa1431850	0.821436	4.041848e-53	-8.615850e-47
Msa0329620	Msa1431850	0.919747	3.055569e-87	-8.615850e-47
Msa0333450	Msa1431850	0.826048	3.359475e-54	-8.615850e-47
Msa0333700	Msa1431850	0.843171	1.650959e-58	-8.615850e-47
Msa0338970	Msa1431850	0.817955	2.521583e-52	-8.615850e-47
Msa0340480	Msa1431850	0.821405	4.108609e-53	-8.615850e-47
Msa0343660	Msa1431850	0.868797	4.999820e-66	-8.615850e-47
Msa0344570	Msa1431850	0.802371	5.792532e-49	-8.615850e-47
Msa0351770	Msa1431850	0.823354	1.448836e-53	-8.615850e-47
Msa0359250	Msa1431850	0.926481	4.422647e-91	-8.615850e-47
Msa1125810	Msa1431850	0.826946	2.051777e-54	-8.615850e-47
Msa1127140	Msa1431850	0.846711	1.829996e-59	-8.615850e-47
Msa1129960	Msa1431850	0.835287	1.831775e-56	-8.615850e-47
Msa1129970	Msa1431850	0.839292	1.728961e-57	-8.615850e-47
Msa1130010	Msa1431850	0.829952	3.860470e-55	-8.615850e-47
Msa1134660	Msa1431850	0.804347	2.256444e-49	-8.615850e-47
Msa1135380	Msa1431850	0.839854	1.235326e-57	-8.615850e-47
Msa1140640	Msa1431850	0.824504	7.788464e-54	-8.615850e-47
Msa1145020	Msa1431850	0.837417	5.262575e-57	-8.615850e-47
Msa1166400	Msa1431850	0.835993	1.214322e-56	-8.615850e-47
Msa1171310	Msa1431850	0.843714	1.182145e-58	-8.615850e-47
Msa1173310	Msa1431850	0.811605	6.439153e-51	-8.615850e-47
Msa1176160	Msa1431850	0.843834	1.098054e-58	-8.615850e-47
Msa1176580	Msa1431850	0.816648	4.961616e-52	-8.615850e-47
Msa1179280	Msa1431850	0.813969	1.955072e-51	-8.615850e-47
Msa1200440	Msa1431850	0.813941	1.983706e-51	-8.615850e-47
Msa1201110	Msa1431850	0.869018	4.239538e-66	-8.615850e-47
Msa1201160	Msa1431850	0.896825	2.543405e-76	-8.615850e-47
Msa0487570	Msa1431850	0.839483	1.542315e-57	-8.615850e-47
Msa0487630	Msa1431850	0.827992	1.151631e-54	-8.615850e-47
Msa0492190	Msa1431850	0.917571	4.512947e-86	-8.615850e-47
Msa0515950	Msa1431850	0.892652	1.302517e-74	-8.615850e-47
Msa0523000	Msa1431850	0.803435	3.490978e-49	-8.615850e-47
Msa0534930	Msa1431850	0.860101	2.611566e-63	-8.615850e-47
Msa0535040	Msa1431850	0.839614	1.425736e-57	-8.615850e-47
Msa0537920	Msa1431850	0.832149	1.114775e-55	-8.615850e-47
Msa0538220	Msa1431850	0.821491	3.924379e-53	-8.615850e-47
Msa0575740	Msa1431850	0.833306	5.751425e-56	-8.615850e-47
Msa0591790	Msa1431850	0.879808	9.206058e-70	-8.615850e-47
Msa0591800	Msa1431850	0.811476	6.868596e-51	-8.615850e-47
Msa0594090	Msa1431850	0.896537	3.355514e-76	-8.615850e-47
Msa0844560	Msa1431850	0.814249	1.696086e-51	-8.615850e-47
Msa0849280	Msa1431850	0.854153	1.487456e-61	-8.615850e-47
Msa0851020	Msa1431850	0.860370	2.165876e-63	-8.615850e-47
Msa0860160	Msa1431850	0.847054	1.474312e-59	-8.615850e-47
Msa0860170	Msa1431850	0.807343	5.293399e-50	-8.615850e-47
Msa0860280	Msa1431850	0.938982	2.727549e-99	-8.615850e-47
Msa0867350	Msa1431850	0.871135	8.605498e-67	-8.615850e-47
Msa0896350	Msa1431850	0.810914	9.093577e-51	-8.615850e-47
Msa0898730	Msa1431850	0.876755	1.085249e-68	-8.615850e-47
Msa0911540	Msa1431850	0.801251	9.833423e-49	-8.615850e-47
Msa0916640	Msa1431850	0.815352	9.660375e-52	-8.615850e-47
Msa0922750	Msa1431850	0.815962	7.065555e-52	-8.615850e-47
Msa0929870	Msa1431850	0.854729	1.013658e-61	-8.615850e-47
Msa0941820	Msa1431850	0.803903	2.791828e-49	-8.615850e-47
Msa0944760	Msa1431850	0.859262	4.672654e-63	-8.615850e-47
Msa0944880	Msa1431850	0.838897	2.189027e-57	-8.615850e-47
Msa0946210	Msa1431850	0.804196	2.426107e-49	-8.615850e-47
Msa0953540	Msa1431850	0.820041	8.456212e-53	-8.615850e-47
Msa0961560	Msa1431850	0.846227	2.478890e-59	-8.615850e-47
Msa0964840	Msa1431850	0.822734	2.021265e-53	-8.615850e-47
Msa0965310	Msa1431850	0.810285	1.243491e-50	-8.615850e-47
Msa0979510	Msa1431850	0.804182	2.441807e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431850	MtrunA17_Chr7g0274631	97.647	170	3	1	1	169	1	170	3.14e-124	346
Msa1431850	MtrunA17_Chr5g0431211	72.263	137	31	4	26	158	36	169	8.43e-68	205
Msa1431850	MtrunA17_Chr4g0051251	68.571	140	37	4	25	160	44	180	2.27e-64	197
Msa1431850	MtrunA17_Chr1g0167411	73.438	128	28	3	27	151	65	189	3.21e-64	196
Msa1431850	MtrunA17_Chr2g0283861	71.318	129	31	3	25	150	33	158	7.14e-63	191
Msa1431850	MtrunA17_Chr1g0190231	73.288	146	33	3	27	169	40	182	1.66e-62	191
Msa1431850	MtrunA17_Chr2g0325831	65.306	147	45	3	16	159	12	155	3.45e-62	189
Msa1431850	MtrunA17_Chr2g0315791	68.217	129	35	3	25	150	31	156	4.06e-60	184
Msa1431850	MtrunA17_Chr1g0187671	70.213	141	36	3	19	156	45	182	7.23e-60	184
Msa1431850	MtrunA17_Chr4g0041141	66.667	129	37	3	25	150	27	152	1.41e-59	183
Msa1431850	MtrunA17_Chr7g0261381	72.263	137	32	3	23	156	60	193	3.61e-59	183
Msa1431850	MtrunA17_Chr1g0183761	73.684	133	29	3	27	156	45	174	1.18e-58	182
Msa1431850	MtrunA17_Chr3g0105921	68.276	145	39	4	27	167	42	183	1.15e-56	177
Msa1431850	MtrunA17_Chr8g0379061	68.462	130	33	4	25	150	83	208	5.48e-47	153
Msa1431850	MtrunA17_Chr1g0211641	71.739	46	12	1	20	65	64	108	6.14e-18	74.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431850	AT5G58500.1	70.423	142	36	4	26	163	18	157	1.71e-67	203
Msa1431850	AT1G07090.1	65.493	142	43	3	26	164	30	168	1.55e-64	196
Msa1431850	AT5G28490.1	76.471	136	26	3	27	159	25	157	1.67e-62	191
Msa1431850	AT3G04510.1	70.714	140	35	3	27	163	33	169	8.90e-60	184
Msa1431850	AT2G42610.1	67.969	128	35	3	26	150	24	148	2.65e-59	182
Msa1431850	AT2G42610.2	67.969	128	35	3	26	150	24	148	2.65e-59	182
Msa1431850	AT2G31160.1	75.556	135	26	4	27	157	54	185	4.71e-58	181
Msa1431850	AT4G18610.1	65.116	129	38	3	26	150	36	161	2.26e-57	178
Msa1431850	AT3G23290.2	75.573	131	26	3	23	150	44	171	2.79e-57	178
Msa1431850	AT1G78815.1	63.566	129	41	3	25	150	38	163	3.08e-55	173
Msa1431850	AT1G16910.1	58.955	134	49	3	25	155	21	151	3.35e-52	164

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCACTACCTTGATCAATT+TGG	0.137888	tig0026913:-50869	Msa1431850:CDS
AATCCAACTTGTCCTTTCTT+TGG	0.215866	tig0026913:-50830	Msa1431850:CDS
TGTTCCAGTCTCTCCTCTTT+TGG	0.217847	tig0026913:+50972	None:intergenic
ATCCAACTTGTCCTTTCTTT+GGG	0.324334	tig0026913:-50829	Msa1431850:CDS
TTCACTACCTTGATCAATTT+GGG	0.326576	tig0026913:-50868	Msa1431850:CDS
TTGTCCTCTCCGTCAGGCTT+GGG	0.343185	tig0026913:-50780	Msa1431850:CDS
CTTGTCCTCTCCGTCAGGCT+TGG	0.356689	tig0026913:-50781	Msa1431850:CDS
CATGGTGGAAGTAACCCTTT+TGG	0.395005	tig0026913:-50704	Msa1431850:CDS
GCACATGGAGATGGAGGGTT+TGG	0.411549	tig0026913:+50803	None:intergenic
CACTTCCATTGTAGCTGCTA+GGG	0.441671	tig0026913:+51032	None:intergenic
GTCGATGGTTTCTTAAGTAC+TGG	0.444811	tig0026913:+50942	None:intergenic
ATGTGCTTGTCCTCTCCGTC+AGG	0.459665	tig0026913:-50786	Msa1431850:CDS
TGATCAATTTGGGAAGACTA+AGG	0.465045	tig0026913:-50858	Msa1431850:CDS
GTGGTGGCAACAAGATCAAC+AGG	0.470799	tig0026913:-51003	Msa1431850:CDS
CTAACAGCGCCATCACCAAA+AGG	0.486109	tig0026913:+50689	None:intergenic
GAGTTAGCTACCATAAGAAA+AGG	0.502671	tig0026913:-50622	Msa1431850:CDS
TAGTCTTCCCAAATTGATCA+AGG	0.503689	tig0026913:+50861	None:intergenic
GTAGTTGTGATGTTGCGGTT+AGG	0.503903	tig0026913:+50593	None:intergenic
AGAGCCAAAAGAGGAGAGAC+TGG	0.512337	tig0026913:-50976	Msa1431850:CDS
GCTTATGATCAAGAGATTCA+TGG	0.517251	tig0026913:-50722	Msa1431850:CDS
ATTGAAGCAGAAAGTGGTAC+TGG	0.535622	tig0026913:+50920	None:intergenic
TCACTTCCATTGTAGCTGCT+AGG	0.535763	tig0026913:+51031	None:intergenic
TTATACAAGAAATATGGCAT+TGG	0.537262	tig0026913:-51074	None:intergenic
CTTTCTGCTTCAATGTGCAA+TGG	0.545802	tig0026913:-50911	Msa1431850:CDS
TGTCCTCTCCGTCAGGCTTG+GGG	0.552431	tig0026913:-50779	Msa1431850:CDS
GGTAGCCTTGATGCTCTCAT+CGG	0.556787	tig0026913:-50758	Msa1431850:CDS
AGAGGACAAGCACATGGAGA+TGG	0.557220	tig0026913:+50794	None:intergenic
CAAATTGATCAAGGTAGTGA+AGG	0.557585	tig0026913:+50870	None:intergenic
GGGTTTGGCATCCCAAAGAA+AGG	0.560750	tig0026913:+50818	None:intergenic
ACAAACCCTAGCAGCTACAA+TGG	0.562580	tig0026913:-51037	Msa1431850:CDS
TTGCACATTGAAGCAGAAAG+TGG	0.564133	tig0026913:+50914	None:intergenic
TAACAGCGCCATCACCAAAA+GGG	0.569402	tig0026913:+50690	None:intergenic
CATGACAAATAGAAATCAAG+AGG	0.576026	tig0026913:+50559	None:intergenic
GGACAAGCACATGGAGATGG+AGG	0.578694	tig0026913:+50797	None:intergenic
ATCCCAAAGAAAGGACAAGT+TGG	0.587746	tig0026913:+50827	None:intergenic
CAGAAAGTGGTACTGGTCGA+TGG	0.588396	tig0026913:+50927	None:intergenic
GAGAAGTAGTTGTGATGTTG+CGG	0.588969	tig0026913:+50588	None:intergenic
CAAGGCTACCCCAAGCCTGA+CGG	0.597531	tig0026913:+50771	None:intergenic
ACAGGTACGAGAGCCAAAAG+AGG	0.613687	tig0026913:-50985	Msa1431850:CDS
TGACGGAGAGGACAAGCACA+TGG	0.624357	tig0026913:+50788	None:intergenic
AATGGAAGTGATTCAAGTGG+TGG	0.625867	tig0026913:-51019	Msa1431850:CDS
GGCGACCGATGAGAGCATCA+AGG	0.637841	tig0026913:+50753	None:intergenic
GACAAGCACATGGAGATGGA+GGG	0.652537	tig0026913:+50798	None:intergenic
CTACCCCAAGCCTGACGGAG+AGG	0.655871	tig0026913:+50776	None:intergenic
TACAATGGAAGTGATTCAAG+TGG	0.673612	tig0026913:-51022	Msa1431850:CDS
CTTGATCATAAGCAGCGCGG+AGG	0.674942	tig0026913:+50732	None:intergenic
TATGATCAAGAGATTCATGG+TGG	0.715975	tig0026913:-50719	Msa1431850:CDS
TCTCTTGATCATAAGCAGCG+CGG	0.775865	tig0026913:+50729	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTCACTACCTTGATCAATTT+GGG	-	tig0026913:50767-50786	Msa1431850:CDS	30.0%
	TACAATGGAAGTGATTCAAG+TGG	-	tig0026913:50613-50632	Msa1431850:CDS	35.0%
	CAAATTGATCAAGGTAGTGA+AGG	+	tig0026913:50768-50787	None:intergenic	35.0%
	CTTCACTACCTTGATCAATT+TGG	-	tig0026913:50766-50785	Msa1431850:CDS	35.0%
	TAGTCTTCCCAAATTGATCA+AGG	+	tig0026913:50777-50796	None:intergenic	35.0%
	TGATCAATTTGGGAAGACTA+AGG	-	tig0026913:50777-50796	Msa1431850:CDS	35.0%
	AATCCAACTTGTCCTTTCTT+TGG	-	tig0026913:50805-50824	Msa1431850:CDS	35.0%
	ATCCAACTTGTCCTTTCTTT+GGG	-	tig0026913:50806-50825	Msa1431850:CDS	35.0%
	GCTTATGATCAAGAGATTCA+TGG	-	tig0026913:50913-50932	Msa1431850:CDS	35.0%
	TATGATCAAGAGATTCATGG+TGG	-	tig0026913:50916-50935	Msa1431850:CDS	35.0%
!	TGTTCGTGATTTTCAGTCAA+AGG	-	tig0026913:50984-51003	Msa1431850:CDS	35.0%
	GAGTTAGCTACCATAAGAAA+AGG	-	tig0026913:51013-51032	Msa1431850:CDS	35.0%
!	TAGGTCTCTTCCTTTTCTTA+TGG	+	tig0026913:51026-51045	None:intergenic	35.0%
	AATGGAAGTGATTCAAGTGG+TGG	-	tig0026913:50616-50635	Msa1431850:CDS	40.0%
	GTCGATGGTTTCTTAAGTAC+TGG	+	tig0026913:50696-50715	None:intergenic	40.0%
	ATTGAAGCAGAAAGTGGTAC+TGG	+	tig0026913:50718-50737	None:intergenic	40.0%
	TTGCACATTGAAGCAGAAAG+TGG	+	tig0026913:50724-50743	None:intergenic	40.0%
	CTTTCTGCTTCAATGTGCAA+TGG	-	tig0026913:50724-50743	Msa1431850:CDS	40.0%
	ATCCCAAAGAAAGGACAAGT+TGG	+	tig0026913:50811-50830	None:intergenic	40.0%
!	GATTTTCAGTCAAAGGCTAG+AGG	-	tig0026913:50991-51010	Msa1431850:CDS	40.0%
	GAGAAGTAGTTGTGATGTTG+CGG	+	tig0026913:51050-51069	None:intergenic	40.0%
	ACAAACCCTAGCAGCTACAA+TGG	-	tig0026913:50598-50617	Msa1431850:CDS	45.0%
	CACTTCCATTGTAGCTGCTA+GGG	+	tig0026913:50606-50625	None:intergenic	45.0%
	TCACTTCCATTGTAGCTGCT+AGG	+	tig0026913:50607-50626	None:intergenic	45.0%
!	TGTTCCAGTCTCTCCTCTTT+TGG	+	tig0026913:50666-50685	None:intergenic	45.0%
	TCTCTTGATCATAAGCAGCG+CGG	+	tig0026913:50909-50928	None:intergenic	45.0%
!!	CATGGTGGAAGTAACCCTTT+TGG	-	tig0026913:50931-50950	Msa1431850:CDS	45.0%
!	GGAAGTAACCCTTTTGGTGA+TGG	-	tig0026913:50937-50956	Msa1431850:CDS	45.0%
	TAACAGCGCCATCACCAAAA+GGG	+	tig0026913:50948-50967	None:intergenic	45.0%
	GTAGTTGTGATGTTGCGGTT+AGG	+	tig0026913:51045-51064	None:intergenic	45.0%
	GTGGTGGCAACAAGATCAAC+AGG	-	tig0026913:50632-50651	Msa1431850:CDS	50.0%
	ACAGGTACGAGAGCCAAAAG+AGG	-	tig0026913:50650-50669	Msa1431850:CDS	50.0%
	AGAGCCAAAAGAGGAGAGAC+TGG	-	tig0026913:50659-50678	Msa1431850:CDS	50.0%
!!	CAGAAAGTGGTACTGGTCGA+TGG	+	tig0026913:50711-50730	None:intergenic	50.0%
	GGGTTTGGCATCCCAAAGAA+AGG	+	tig0026913:50820-50839	None:intergenic	50.0%
	GACAAGCACATGGAGATGGA+GGG	+	tig0026913:50840-50859	None:intergenic	50.0%
	AGAGGACAAGCACATGGAGA+TGG	+	tig0026913:50844-50863	None:intergenic	50.0%
!	GGTAGCCTTGATGCTCTCAT+CGG	-	tig0026913:50877-50896	Msa1431850:CDS	50.0%
	CTAACAGCGCCATCACCAAA+AGG	+	tig0026913:50949-50968	None:intergenic	50.0%
!!!	CTTTTGGTGATGGCGCTGTT+AGG	-	tig0026913:50947-50966	Msa1431850:CDS	50.0%
	GCACATGGAGATGGAGGGTT+TGG	+	tig0026913:50835-50854	None:intergenic	55.0%
	GGACAAGCACATGGAGATGG+AGG	+	tig0026913:50841-50860	None:intergenic	55.0%
	TGACGGAGAGGACAAGCACA+TGG	+	tig0026913:50850-50869	None:intergenic	55.0%
	ATGTGCTTGTCCTCTCCGTC+AGG	-	tig0026913:50849-50868	Msa1431850:CDS	55.0%
	TTGTCCTCTCCGTCAGGCTT+GGG	-	tig0026913:50855-50874	Msa1431850:CDS	55.0%
	CTTGATCATAAGCAGCGCGG+AGG	+	tig0026913:50906-50925	None:intergenic	55.0%
	CTTGTCCTCTCCGTCAGGCT+TGG	-	tig0026913:50854-50873	Msa1431850:CDS	60.0%
	TGTCCTCTCCGTCAGGCTTG+GGG	-	tig0026913:50856-50875	Msa1431850:CDS	60.0%
	CAAGGCTACCCCAAGCCTGA+CGG	+	tig0026913:50867-50886	None:intergenic	60.0%
!!	GGCGACCGATGAGAGCATCA+AGG	+	tig0026913:50885-50904	None:intergenic	60.0%
	CTACCCCAAGCCTGACGGAG+AGG	+	tig0026913:50862-50881	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0026913	gene	50574	51083	50574	ID=Msa1431850;Name=Msa1431850
tig0026913	mRNA	50574	51083	50574	ID=Msa1431850-mRNA-1;Parent=Msa1431850;Name=Msa1431850-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|169
tig0026913	exon	50574	51083	50574	ID=Msa1431850-mRNA-1:exon:7764;Parent=Msa1431850-mRNA-1
tig0026913	CDS	50574	51083	50574	ID=Msa1431850-mRNA-1:cds;Parent=Msa1431850-mRNA-1

Gene Sequence

>Msa1431850

ATGGCATTGGTTTCAGCTGAATCAACAAACCCTAGCAGCTACAATGGAAGTGATTCAAGTGGTGGCAACAAGATCAACAGGTACGAGAGCCAAAAGAGGAGAGACTGGAACACATTCTGCCAGTACTTAAGAAACCATCGACCAGTACCACTTTCTGCTTCAATGTGCAATGGAACTCATGTTCTTGAATTCCTTCACTACCTTGATCAATTTGGGAAGACTAAGGTTCACAATCCAACTTGTCCTTTCTTTGGGATGCCAAACCCTCCATCTCCATGTGCTTGTCCTCTCCGTCAGGCTTGGGGTAGCCTTGATGCTCTCATCGGTCGCCTCCGCGCTGCTTATGATCAAGAGATTCATGGTGGAAGTAACCCTTTTGGTGATGGCGCTGTTAGGTTTTACTTGCGTGATGTTCGTGATTTTCAGTCAAAGGCTAGAGGAGTTAGCTACCATAAGAAAAGGAAGAGACCTAACCGCAACATCACAACTACTTCTCAATCATCCTCTTGA

Protein sequence

>Msa1431850

MALVSAESTNPSSYNGSDSSGGNKINRYESQKRRDWNTFCQYLRNHRPVPLSASMCNGTHVLEFLHYLDQFGKTKVHNPTCPFFGMPNPPSPCACPLRQAWGSLDALIGRLRAAYDQEIHGGSNPFGDGAVRFYLRDVRDFQSKARGVSYHKKRKRPNRNITTTSQSSS*