AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000190.t1 MTR_2g062370 87.946 589 37 3 4 589 32 589 0.0 1041
MS.gene000190.t1 MTR_2g062380 84.428 533 83 0 58 590 28 560 0.0 947
MS.gene000190.t1 MTR_2g062410 76.610 590 102 3 1 589 31 585 0.0 919
MS.gene000190.t1 MTR_2g062430 68.557 582 140 4 9 589 13 552 0.0 829
MS.gene000190.t1 MTR_4g131710 68.803 593 149 4 1 590 27 586 0.0 817
MS.gene000190.t1 MTR_2g067640 56.309 531 201 8 70 590 32 541 0.0 602
MS.gene000190.t1 MTR_2g062340 51.601 531 200 10 70 589 32 516 8.70e-179 516
MS.gene000190.t1 MTR_1g080370 45.911 538 261 9 70 590 35 559 1.34e-163 478
MS.gene000190.t1 MTR_1g080410 45.911 538 261 9 70 590 35 559 1.34e-163 478
MS.gene000190.t1 MTR_5g007860 43.693 547 269 9 70 589 71 605 4.75e-154 456
MS.gene000190.t1 MTR_4g077620 44.712 539 268 11 70 589 40 567 1.98e-148 440
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000190.t1 AT2G44260 53.726 577 254 6 15 589 17 582 0.0 632
MS.gene000190.t1 AT2G44260 57.854 522 208 5 70 589 41 552 0.0 623
MS.gene000190.t1 AT2G44230 53.333 525 227 6 69 589 31 541 0.0 567
MS.gene000190.t1 AT3G01870 48.674 528 248 7 70 589 71 583 2.57e-176 512
MS.gene000190.t1 AT3G01880 48.393 529 250 8 70 589 75 589 1.90e-172 502
MS.gene000190.t1 AT5G18490 44.944 534 263 9 70 590 37 552 3.44e-159 467
MS.gene000190.t1 AT3G04350 44.015 543 270 10 69 590 15 544 8.01e-154 453
MS.gene000190.t1 AT3G04350 44.096 542 269 10 70 590 38 566 3.48e-153 452
MS.gene000190.t1 AT5G43950 41.728 544 294 8 62 589 28 564 1.37e-144 430
MS.gene000190.t1 AT1G04090 41.392 546 283 12 70 589 36 570 1.91e-141 422

Find 140 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACTCCCCTTTGTATATT+TGG 0.124214 2.1:+33596955 MS.gene000190:CDS
GCTTTCCCAGGTCCATTAAA+AGG 0.194984 2.1:-33597614 None:intergenic
ATTGGTTTCTGCAGAGTATT+TGG 0.229797 2.1:+33597973 MS.gene000190:CDS
CTTGTAGCAAAGGATATTTC+AGG 0.255851 2.1:+33596858 MS.gene000190:CDS
AGGTAGCAATGTTATGGATA+TGG 0.258056 2.1:+33597940 MS.gene000190:CDS
GTGAGTCTTCTACATGGATT+TGG 0.274745 2.1:+33597090 MS.gene000190:CDS
CATCAACATTCAGCAAAGTT+TGG 0.275579 2.1:+33596709 MS.gene000190:CDS
TCCTCTCCCAGCACTTCTTT+TGG 0.282938 2.1:-33598145 None:intergenic
ATTGGGAGCATGTGACATTA+AGG 0.285039 2.1:+33597690 MS.gene000190:CDS
GTCCAAAGGAAAAGGGTAAT+TGG 0.292083 2.1:+33598179 MS.gene000190:CDS
CATGACTGTGTTTGCGAAAC+TGG 0.292140 2.1:-33596658 None:intergenic
AATTTGAAGTTAGGGAAAAT+AGG 0.310027 2.1:+33597653 MS.gene000190:CDS
ATCAACATTCAGCAAAGTTT+GGG 0.318101 2.1:+33596710 MS.gene000190:CDS
GGTACCTTCGTTGCTCAAAA+TGG 0.332082 2.1:+33597185 MS.gene000190:CDS
GAGTTCATAAATTTGAAGTT+AGG 0.341852 2.1:+33597644 MS.gene000190:CDS
TGAGTCTTCTACATGGATTT+GGG 0.342802 2.1:+33597091 MS.gene000190:CDS
GCTTTAGTTCCTCTATTGAT+TGG 0.347877 2.1:-33597146 None:intergenic
CTCGAAGAAGTTTGTGCAAT+AGG 0.348542 2.1:-33596534 None:intergenic
GAACCAACGGGACTGACTTA+TGG 0.352846 2.1:+33596777 MS.gene000190:CDS
GACCTGTGGTTTATGCTTCT+CGG 0.360096 2.1:+33597837 MS.gene000190:CDS
CATGCTGGCCTTCATCTACT+AGG 0.369862 2.1:+33597881 MS.gene000190:CDS
CTGACACACATGACTTTGTT+GGG 0.371564 2.1:-33597047 None:intergenic
CTCAACATAATGGTGGTTCA+TGG 0.380091 2.1:+33597753 MS.gene000190:CDS
GTCTATTTCTCTCAACATAA+TGG 0.382864 2.1:+33597743 MS.gene000190:CDS
TCTGACACACATGACTTTGT+TGG 0.395634 2.1:-33597048 None:intergenic
CTAACAATGATGGTGCCTAT+TGG 0.409794 2.1:+33597459 MS.gene000190:CDS
CAATAGGCACCATCATTGTT+AGG 0.412763 2.1:-33597458 None:intergenic
TGATAAAGGTAGCAATGTTA+TGG 0.414830 2.1:+33597934 MS.gene000190:CDS
AGTTCATAAATTTGAAGTTA+GGG 0.416307 2.1:+33597645 MS.gene000190:CDS
ACTTACCTTCCTCTGTCAAT+TGG 0.417119 2.1:+33597345 MS.gene000190:CDS
ACTTATGGATACTCCATGTT+AGG 0.419517 2.1:+33596792 MS.gene000190:CDS
GTCTCAATTTGTGTTAGATT+AGG 0.420473 2.1:-33597272 None:intergenic
CCTGTTGCAATAGTACAAAA+TGG 0.426079 2.1:+33597416 MS.gene000190:CDS
AGCTTGAATGTGTGGTCAAT+GGG 0.428759 2.1:-33596510 None:intergenic
GCAAGAGATGATACTGATAA+AGG 0.429248 2.1:+33597920 MS.gene000190:CDS
CTTCATCAGGATGCAAGTAT+AGG 0.432505 2.1:-33597319 None:intergenic
GGATATGGGAAAATATGTAT+TGG 0.435630 2.1:+33597955 MS.gene000190:CDS
TCTTTATTTGCATCATCAAC+AGG 0.436217 2.1:-33597491 None:intergenic
ACCAACAGGTCCAAAGGAAA+AGG 0.437204 2.1:+33598171 MS.gene000190:CDS
GAAGGTAAGTATTCTTCATC+AGG 0.444599 2.1:-33597332 None:intergenic
GGGTTTACCAAAAGAAGTGC+TGG 0.449038 2.1:+33598138 MS.gene000190:CDS
GGTAGCAATGTTATGGATAT+GGG 0.449769 2.1:+33597941 MS.gene000190:CDS
AATGTTGAGAAGTTATTGCC+TGG 0.454803 2.1:+33598082 MS.gene000190:CDS
ATCAGTTTGAAACTCAAGTT+GGG 0.455255 2.1:-33597786 None:intergenic
GTACATGTGAAACCAATGTT+TGG 0.455920 2.1:+33597554 MS.gene000190:CDS
TATCAGTTTGAAACTCAAGT+TGG 0.456320 2.1:-33597787 None:intergenic
AATACTAGTGAGTAATCAAC+AGG 0.461578 2.1:-33596906 None:intergenic
CTTGTATAGTAACGCTCCAT+TGG 0.467093 2.1:-33597375 None:intergenic
ATAGGAATGGAGAGTAAGAG+TGG 0.472282 2.1:-33597301 None:intergenic
TAGGTGAACATGTTGGTGAT+TGG 0.478019 2.1:+33597672 MS.gene000190:CDS
GCAAACACAGTCATGAATCT+AGG 0.478598 2.1:+33596666 MS.gene000190:CDS
TGGTAGCCATCAGGTGCTAT+TGG 0.479434 2.1:-33596981 None:intergenic
CTGGGAGAGGAAGGACCAAC+AGG 0.482050 2.1:+33598157 MS.gene000190:CDS
GGAAGGTTTGTCTTGTGTTG+TGG 0.484281 2.1:-33597024 None:intergenic
GTGGTTTATGCTTCTCGGCA+TGG 0.484909 2.1:+33597842 MS.gene000190:CDS
ACTGAGGCGCCTTGATCGTC+CGG 0.485272 2.1:-33596750 None:intergenic
TTGATGATGCAAATAAAGAA+AGG 0.492381 2.1:+33597495 MS.gene000190:CDS
TTGGTAGCCAAATATACAAA+GGG 0.494599 2.1:-33596962 None:intergenic
AACAGGTAGGTCAAGCCAAT+AGG 0.497340 2.1:-33597474 None:intergenic
CTTTCCCAGGTCCATTAAAA+GGG 0.501041 2.1:-33597613 None:intergenic
GTGGTCAATGGGGCGAGAGC+GGG 0.505037 2.1:-33596499 None:intergenic
GACATTAAGGGTAAGTAACT+TGG 0.505898 2.1:+33597703 MS.gene000190:CDS
TGGATATGTTCTTGTAGCAA+AGG 0.506215 2.1:+33596848 MS.gene000190:CDS
TTGGCTGTAGCTTCCTAACA+TGG 0.507947 2.1:-33596805 None:intergenic
AGGTGAACATGTTGGTGATT+GGG 0.511142 2.1:+33597673 MS.gene000190:CDS
GAAGGTACCAACGCGAACAC+CGG 0.518336 2.1:-33597171 None:intergenic
GCCTCAGTGTATGAACCAAC+GGG 0.521228 2.1:+33596765 MS.gene000190:CDS
GAAGGTGGACCGGACGATCA+AGG 0.523730 2.1:+33596741 MS.gene000190:CDS
AGTGTTGAATTGCAACATAC+AGG 0.524704 2.1:+33596633 MS.gene000190:CDS
AAGCTTGAATGTGTGGTCAA+TGG 0.525417 2.1:-33596511 None:intergenic
TTGGGAGCATGTGACATTAA+GGG 0.526297 2.1:+33597691 MS.gene000190:CDS
CCTCAGGATCCTAACAATGA+TGG 0.528215 2.1:+33597449 MS.gene000190:CDS
CCTACAGCATGGTAGCCATC+AGG 0.530924 2.1:-33596990 None:intergenic
GTGAATGTTCCTCCAAACAT+TGG 0.533393 2.1:-33597566 None:intergenic
CCAACAGGTCCAAAGGAAAA+GGG 0.535094 2.1:+33598172 MS.gene000190:CDS
AAGGTACCAACGCGAACACC+GGG 0.535734 2.1:-33597170 None:intergenic
TTCAAACATGAAATTGATGG+AGG 0.536359 2.1:-33597221 None:intergenic
ACATGAAATTGATGGAGGGT+TGG 0.537969 2.1:-33597216 None:intergenic
AAAGAGGTTTGTTGTTGGGT+TGG 0.542937 2.1:-33596824 None:intergenic
GTAACTTGGATGGTCAGTTA+TGG 0.544808 2.1:+33597717 MS.gene000190:CDS
AAATGGTACTAACCTTCCTC+AGG 0.545862 2.1:+33597433 MS.gene000190:CDS
GGAAGGACCAACAGGTCCAA+AGG 0.546506 2.1:+33598165 MS.gene000190:CDS
TCAGGATGCAAGTATAGGAA+TGG 0.556685 2.1:-33597314 None:intergenic
TCTAGACTATAGTCAATATG+TGG 0.557808 2.1:-33598046 None:intergenic
AAAGAAGTGCTGGGAGAGGA+AGG 0.559112 2.1:+33598148 MS.gene000190:CDS
ATGTTGAGAAGTTATTGCCT+GGG 0.560794 2.1:+33598083 MS.gene000190:CDS
CGCCTCAGTGTATGAACCAA+CGG 0.561230 2.1:+33596764 MS.gene000190:CDS
GCACAAACTTCTTCGAGTGA+AGG 0.562526 2.1:+33596540 MS.gene000190:CDS
ATGCTGGCCTTCATCTACTA+GGG 0.562857 2.1:+33597882 MS.gene000190:CDS
TGTGGTCAATGGGGCGAGAG+CGG 0.565396 2.1:-33596500 None:intergenic
TGATGATGCAAATAAAGAAA+GGG 0.567205 2.1:+33597496 MS.gene000190:CDS
AAGGAAAAGGGTAATTGGAA+TGG 0.567477 2.1:+33598184 MS.gene000190:CDS
AATAGAGGAACTAAAGCTCC+CGG 0.568897 2.1:+33597152 MS.gene000190:CDS
TAATACATTATAGTGAATGC+AGG 0.571622 2.1:+33596598 MS.gene000190:CDS
CATTGTTAGGATCCTGAGGA+AGG 0.572703 2.1:-33597445 None:intergenic
TCAAACATGAAATTGATGGA+GGG 0.575731 2.1:-33597220 None:intergenic
CATGCATCATATCCACATGC+TGG 0.576287 2.1:+33597866 MS.gene000190:CDS
GTTTGGGGCGCCTACGAAGG+TGG 0.577121 2.1:+33596726 MS.gene000190:CDS
CTTGTGTTGTGGTGACAACA+AGG 0.577689 2.1:-33597013 None:intergenic
TGACACACATGACTTTGTTG+GGG 0.578338 2.1:-33597046 None:intergenic
ATTGGTAGCCAAATATACAA+AGG 0.583699 2.1:-33596963 None:intergenic
GGGCGCCTACGAAGGTGGAC+CGG 0.583899 2.1:+33596731 MS.gene000190:CDS
TATTTGCATCATCAACAGGT+AGG 0.588221 2.1:-33597487 None:intergenic
TCACTATAATGTATTACAAG+AGG 0.590425 2.1:-33596591 None:intergenic
ACCAAAAGAAGTGCTGGGAG+AGG 0.590505 2.1:+33598144 MS.gene000190:CDS
CTGAAGTTAAAGAGCCAGCA+TGG 0.591737 2.1:+33597999 MS.gene000190:CDS
GATGTTAGACCAATCAATAG+AGG 0.591766 2.1:+33597137 MS.gene000190:CDS
ATCGTCCGGTCCACCTTCGT+AGG 0.592536 2.1:-33596736 None:intergenic
TATTTCTCTCAACATAATGG+TGG 0.595363 2.1:+33597746 MS.gene000190:CDS
AAAGTTTGGGGCGCCTACGA+AGG 0.597769 2.1:+33596723 MS.gene000190:CDS
GGGAAAATAGGTGAACATGT+TGG 0.598707 2.1:+33597665 MS.gene000190:CDS
GGAGCGTTACTATACAAGAA+AGG 0.600026 2.1:+33597380 MS.gene000190:CDS
GATATTTCAGGAAAGAGCAA+TGG 0.606860 2.1:+33596870 MS.gene000190:CDS
CCTGATGGCTACCATGCTGT+AGG 0.611461 2.1:+33596990 MS.gene000190:CDS
CATGTGAAACCAATGTTTGG+AGG 0.614379 2.1:+33597557 MS.gene000190:CDS
AGCAATGTGAGTCTTCTACA+TGG 0.615710 2.1:+33597084 MS.gene000190:CDS
GAGTCTTCTACATGGATTTG+GGG 0.615942 2.1:+33597092 MS.gene000190:CDS
GGTTTACCAAAAGAAGTGCT+GGG 0.616870 2.1:+33598139 MS.gene000190:CDS
TATCCATAAGTCAGTCCCGT+TGG 0.618765 2.1:-33596780 None:intergenic
CCATCATTGTTAGGATCCTG+AGG 0.623207 2.1:-33597449 None:intergenic
TGCCGAGAAGCATAAACCAC+AGG 0.627638 2.1:-33597839 None:intergenic
AATAAAGAAAGGGTCAAGCA+AGG 0.630463 2.1:+33597506 MS.gene000190:CDS
TCAACATTCAGCAAAGTTTG+GGG 0.632154 2.1:+33596711 MS.gene000190:CDS
AGTAGATGAAGGCCAGCATG+TGG 0.633143 2.1:-33597878 None:intergenic
TGGCTACCAATAGCACCTGA+TGG 0.636406 2.1:+33596975 MS.gene000190:CDS
AAAGCTCCCGGTGTTCGCGT+TGG 0.645605 2.1:+33597164 MS.gene000190:CDS
ATTGAACACTGCATCCGTCA+CGG 0.650457 2.1:+33596926 MS.gene000190:CDS
TGACAACAAGGCCTACAGCA+TGG 0.652162 2.1:-33597001 None:intergenic
CAATAGGAAGCTTGAATGTG+TGG 0.655769 2.1:-33596518 None:intergenic
TAAACCACAGGTCTTTGTGT+CGG 0.656244 2.1:-33597827 None:intergenic
GACACACATGACTTTGTTGG+GGG 0.656587 2.1:-33597045 None:intergenic
TTAAGGGTAAGTAACTTGGA+TGG 0.663636 2.1:+33597707 MS.gene000190:CDS
GGAGTCTTGATCAACCGTGA+CGG 0.669237 2.1:-33596940 None:intergenic
ACTAGTGAGTAATCAACAGG+TGG 0.691530 2.1:-33596903 None:intergenic
AAGACTCACATTGCTCTGTG+AGG 0.699723 2.1:-33597076 None:intergenic
TGGTAGCCAAATATACAAAG+GGG 0.704831 2.1:-33596961 None:intergenic
CATTCACTGATATTGCCATG+TGG 0.716023 2.1:+33597582 MS.gene000190:CDS
GCTTGAATGTGTGGTCAATG+GGG 0.727664 2.1:-33596509 None:intergenic
TCCCGTTGGTTCATACACTG+AGG 0.727884 2.1:-33596766 None:intergenic
AAAAGGGTAGAAAATCCACA+TGG 0.732112 2.1:-33597597 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTGAAAAAATTATTAT+GGG + chr2.1:33598118-33598137 MS.gene000190:CDS 10.0%
!!! TGTTTTTGAAAAAATTATTA+TGG + chr2.1:33598117-33598136 MS.gene000190:CDS 10.0%
!!! AGTTCATAAATTTGAAGTTA+GGG + chr2.1:33597645-33597664 MS.gene000190:CDS 20.0%
!!! TTTTTCAAACATGAAATTGA+TGG - chr2.1:33597227-33597246 None:intergenic 20.0%
! TAATACATTATAGTGAATGC+AGG + chr2.1:33596598-33596617 MS.gene000190:CDS 25.0%
! TCACTATAATGTATTACAAG+AGG - chr2.1:33596594-33596613 None:intergenic 25.0%
!! AATTTGAAGTTAGGGAAAAT+AGG + chr2.1:33597653-33597672 MS.gene000190:CDS 25.0%
!! GAGTTCATAAATTTGAAGTT+AGG + chr2.1:33597644-33597663 MS.gene000190:CDS 25.0%
!! TGATGATGCAAATAAAGAAA+GGG + chr2.1:33597496-33597515 MS.gene000190:CDS 25.0%
!!! AAAACATCTTTTAATTTCCC+AGG - chr2.1:33598103-33598122 None:intergenic 25.0%
!!! AGCATGGTTGAATTTTTTTA+GGG + chr2.1:33598015-33598034 MS.gene000190:CDS 25.0%
!!! TTGATGATGCAAATAAAGAA+AGG + chr2.1:33597495-33597514 MS.gene000190:CDS 25.0%
AATACTAGTGAGTAATCAAC+AGG - chr2.1:33596909-33596928 None:intergenic 30.0%
ACAAGAACATATCCAAAAAG+AGG - chr2.1:33596843-33596862 None:intergenic 30.0%
ATCAACATTCAGCAAAGTTT+GGG + chr2.1:33596710-33596729 MS.gene000190:CDS 30.0%
CTAAAAAAATTCAACCATGC+TGG - chr2.1:33598016-33598035 None:intergenic 30.0%
GGATATGGGAAAATATGTAT+TGG + chr2.1:33597955-33597974 MS.gene000190:CDS 30.0%
GTCTCAATTTGTGTTAGATT+AGG - chr2.1:33597275-33597294 None:intergenic 30.0%
TATTTCTCTCAACATAATGG+TGG + chr2.1:33597746-33597765 MS.gene000190:CDS 30.0%
TCAAACATGAAATTGATGGA+GGG - chr2.1:33597223-33597242 None:intergenic 30.0%
TCTAGACTATAGTCAATATG+TGG - chr2.1:33598049-33598068 None:intergenic 30.0%
TGATAAAGGTAGCAATGTTA+TGG + chr2.1:33597934-33597953 MS.gene000190:CDS 30.0%
TTCAAACATGAAATTGATGG+AGG - chr2.1:33597224-33597243 None:intergenic 30.0%
! ATCAGTTTGAAACTCAAGTT+GGG - chr2.1:33597789-33597808 None:intergenic 30.0%
! ATTGGTAGCCAAATATACAA+AGG - chr2.1:33596966-33596985 None:intergenic 30.0%
! GTCTATTTCTCTCAACATAA+TGG + chr2.1:33597743-33597762 MS.gene000190:CDS 30.0%
! TATCAGTTTGAAACTCAAGT+TGG - chr2.1:33597790-33597809 None:intergenic 30.0%
! TCTTTATTTGCATCATCAAC+AGG - chr2.1:33597494-33597513 None:intergenic 30.0%
! TGGATTTTCTACCCTTTTAA+TGG + chr2.1:33597602-33597621 MS.gene000190:CDS 30.0%
! TTGGTAGCCAAATATACAAA+GGG - chr2.1:33596965-33596984 None:intergenic 30.0%
!!! CAGCATGGTTGAATTTTTTT+AGG + chr2.1:33598014-33598033 MS.gene000190:CDS 30.0%
!!! GTTGAATTTTTTTAGGGAGT+GGG + chr2.1:33598021-33598040 MS.gene000190:CDS 30.0%
!!! TTGAATTTTTTTAGGGAGTG+GGG + chr2.1:33598022-33598041 MS.gene000190:CDS 30.0%
AAAAACCAATTGACAGAGGA+AGG - chr2.1:33597353-33597372 None:intergenic 35.0%
AAAAGGGTAGAAAATCCACA+TGG - chr2.1:33597600-33597619 None:intergenic 35.0%
AAGACTCCCCTTTGTATATT+TGG + chr2.1:33596955-33596974 MS.gene000190:CDS 35.0%
AAGGAAAAGGGTAATTGGAA+TGG + chr2.1:33598184-33598203 MS.gene000190:CDS 35.0%
AATAAAGAAAGGGTCAAGCA+AGG + chr2.1:33597506-33597525 MS.gene000190:CDS 35.0%
AATGTTGAGAAGTTATTGCC+TGG + chr2.1:33598082-33598101 MS.gene000190:CDS 35.0%
ACTTATGGATACTCCATGTT+AGG + chr2.1:33596792-33596811 MS.gene000190:CDS 35.0%
AGGTAGCAATGTTATGGATA+TGG + chr2.1:33597940-33597959 MS.gene000190:CDS 35.0%
ATGTTGAGAAGTTATTGCCT+GGG + chr2.1:33598083-33598102 MS.gene000190:CDS 35.0%
CATCAACATTCAGCAAAGTT+TGG + chr2.1:33596709-33596728 MS.gene000190:CDS 35.0%
CCTGTTGCAATAGTACAAAA+TGG + chr2.1:33597416-33597435 MS.gene000190:CDS 35.0%
CTTCATCTACTAGGGAAAAA+TGG + chr2.1:33597890-33597909 MS.gene000190:CDS 35.0%
CTTGTAGCAAAGGATATTTC+AGG + chr2.1:33596858-33596877 MS.gene000190:CDS 35.0%
GAAGGTAAGTATTCTTCATC+AGG - chr2.1:33597335-33597354 None:intergenic 35.0%
GACATTAAGGGTAAGTAACT+TGG + chr2.1:33597703-33597722 MS.gene000190:CDS 35.0%
GATATTTCAGGAAAGAGCAA+TGG + chr2.1:33596870-33596889 MS.gene000190:CDS 35.0%
GATGTTAGACCAATCAATAG+AGG + chr2.1:33597137-33597156 MS.gene000190:CDS 35.0%
GCAAGAGATGATACTGATAA+AGG + chr2.1:33597920-33597939 MS.gene000190:CDS 35.0%
GGTAGCAATGTTATGGATAT+GGG + chr2.1:33597941-33597960 MS.gene000190:CDS 35.0%
GTACATGTGAAACCAATGTT+TGG + chr2.1:33597554-33597573 MS.gene000190:CDS 35.0%
TATTTGCATCATCAACAGGT+AGG - chr2.1:33597490-33597509 None:intergenic 35.0%
TCAACATTCAGCAAAGTTTG+GGG + chr2.1:33596711-33596730 MS.gene000190:CDS 35.0%
TGAGTCTTCTACATGGATTT+GGG + chr2.1:33597091-33597110 MS.gene000190:CDS 35.0%
TGGATATGTTCTTGTAGCAA+AGG + chr2.1:33596848-33596867 MS.gene000190:CDS 35.0%
TGGTAGCCAAATATACAAAG+GGG - chr2.1:33596964-33596983 None:intergenic 35.0%
TTAAGGGTAAGTAACTTGGA+TGG + chr2.1:33597707-33597726 MS.gene000190:CDS 35.0%
! AGTGTTGAATTGCAACATAC+AGG + chr2.1:33596633-33596652 MS.gene000190:CDS 35.0%
! ATTGGTTTCTGCAGAGTATT+TGG + chr2.1:33597973-33597992 MS.gene000190:CDS 35.0%
! CATTTTTCCCTAGTAGATGA+AGG - chr2.1:33597892-33597911 None:intergenic 35.0%
! CCATTTTGTACTATTGCAAC+AGG - chr2.1:33597419-33597438 None:intergenic 35.0%
! GCTTTAGTTCCTCTATTGAT+TGG - chr2.1:33597149-33597168 None:intergenic 35.0%
! TTCCAATTACCCTTTTCCTT+TGG - chr2.1:33598184-33598203 None:intergenic 35.0%
!! CCAAAAAGAGGTTTGTTGTT+GGG - chr2.1:33596831-33596850 None:intergenic 35.0%
!! TCCAAAAAGAGGTTTGTTGT+TGG - chr2.1:33596832-33596851 None:intergenic 35.0%
!!! GGTTGAATTTTTTTAGGGAG+TGG + chr2.1:33598020-33598039 MS.gene000190:CDS 35.0%
!!! GTCAATTGGTTTTTCTCCAA+TGG + chr2.1:33597359-33597378 MS.gene000190:CDS 35.0%
AAATGGTACTAACCTTCCTC+AGG + chr2.1:33597433-33597452 MS.gene000190:CDS 40.0%
AAGCTTGAATGTGTGGTCAA+TGG - chr2.1:33596514-33596533 None:intergenic 40.0%
AATAGAGGAACTAAAGCTCC+CGG + chr2.1:33597152-33597171 MS.gene000190:CDS 40.0%
ACATGAAATTGATGGAGGGT+TGG - chr2.1:33597219-33597238 None:intergenic 40.0%
ACTAGTGAGTAATCAACAGG+TGG - chr2.1:33596906-33596925 None:intergenic 40.0%
ACTTACCTTCCTCTGTCAAT+TGG + chr2.1:33597345-33597364 MS.gene000190:CDS 40.0%
AGCAATGTGAGTCTTCTACA+TGG + chr2.1:33597084-33597103 MS.gene000190:CDS 40.0%
AGCTTGAATGTGTGGTCAAT+GGG - chr2.1:33596513-33596532 None:intergenic 40.0%
ATAGGAATGGAGAGTAAGAG+TGG - chr2.1:33597304-33597323 None:intergenic 40.0%
CAATAGGAAGCTTGAATGTG+TGG - chr2.1:33596521-33596540 None:intergenic 40.0%
CAATAGGCACCATCATTGTT+AGG - chr2.1:33597461-33597480 None:intergenic 40.0%
CATGTGAAACCAATGTTTGG+AGG + chr2.1:33597557-33597576 MS.gene000190:CDS 40.0%
CATTCACTGATATTGCCATG+TGG + chr2.1:33597582-33597601 MS.gene000190:CDS 40.0%
CTACTAGGGAAAAATGGAGT+AGG + chr2.1:33597896-33597915 MS.gene000190:CDS 40.0%
CTCAACATAATGGTGGTTCA+TGG + chr2.1:33597753-33597772 MS.gene000190:CDS 40.0%
CTCGAAGAAGTTTGTGCAAT+AGG - chr2.1:33596537-33596556 None:intergenic 40.0%
CTGACACACATGACTTTGTT+GGG - chr2.1:33597050-33597069 None:intergenic 40.0%
CTTCATCAGGATGCAAGTAT+AGG - chr2.1:33597322-33597341 None:intergenic 40.0%
CTTGTATAGTAACGCTCCAT+TGG - chr2.1:33597378-33597397 None:intergenic 40.0%
CTTTCCCAGGTCCATTAAAA+GGG - chr2.1:33597616-33597635 None:intergenic 40.0%
GAGTCTTCTACATGGATTTG+GGG + chr2.1:33597092-33597111 MS.gene000190:CDS 40.0%
GCAAACACAGTCATGAATCT+AGG + chr2.1:33596666-33596685 MS.gene000190:CDS 40.0%
GGAGAAAAACCAATTGACAG+AGG - chr2.1:33597357-33597376 None:intergenic 40.0%
GGGAAAATAGGTGAACATGT+TGG + chr2.1:33597665-33597684 MS.gene000190:CDS 40.0%
GTAACTTGGATGGTCAGTTA+TGG + chr2.1:33597717-33597736 MS.gene000190:CDS 40.0%
GTCCAAAGGAAAAGGGTAAT+TGG + chr2.1:33598179-33598198 MS.gene000190:CDS 40.0%
GTGAATGTTCCTCCAAACAT+TGG - chr2.1:33597569-33597588 None:intergenic 40.0%
GTGAGTCTTCTACATGGATT+TGG + chr2.1:33597090-33597109 MS.gene000190:CDS 40.0%
TAAACCACAGGTCTTTGTGT+CGG - chr2.1:33597830-33597849 None:intergenic 40.0%
TCAGGATGCAAGTATAGGAA+TGG - chr2.1:33597317-33597336 None:intergenic 40.0%
TCTGACACACATGACTTTGT+TGG - chr2.1:33597051-33597070 None:intergenic 40.0%
TGACACACATGACTTTGTTG+GGG - chr2.1:33597049-33597068 None:intergenic 40.0%
! ATTGGGAGCATGTGACATTA+AGG + chr2.1:33597690-33597709 MS.gene000190:CDS 40.0%
! CCCAACAACAAACCTCTTTT+TGG + chr2.1:33596828-33596847 MS.gene000190:CDS 40.0%
! CTAACAATGATGGTGCCTAT+TGG + chr2.1:33597459-33597478 MS.gene000190:CDS 40.0%
! GGAGCGTTACTATACAAGAA+AGG + chr2.1:33597380-33597399 MS.gene000190:CDS 40.0%
! TTGGGAGCATGTGACATTAA+GGG + chr2.1:33597691-33597710 MS.gene000190:CDS 40.0%
!! AAAGAGGTTTGTTGTTGGGT+TGG - chr2.1:33596827-33596846 None:intergenic 40.0%
!! AACTCTACTTTTGCTTTCCC+AGG - chr2.1:33597629-33597648 None:intergenic 40.0%
!! AGGTGAACATGTTGGTGATT+GGG + chr2.1:33597673-33597692 MS.gene000190:CDS 40.0%
!! GGTTTACCAAAAGAAGTGCT+GGG + chr2.1:33598139-33598158 MS.gene000190:CDS 40.0%
!! TAGGTGAACATGTTGGTGAT+TGG + chr2.1:33597672-33597691 MS.gene000190:CDS 40.0%
!! TCTACCCTTTTAATGGACCT+GGG + chr2.1:33597609-33597628 MS.gene000190:CDS 40.0%
!! TTCTACCCTTTTAATGGACC+TGG + chr2.1:33597608-33597627 MS.gene000190:CDS 40.0%
AACAGGTAGGTCAAGCCAAT+AGG - chr2.1:33597477-33597496 None:intergenic 45.0%
AAGACTCACATTGCTCTGTG+AGG - chr2.1:33597079-33597098 None:intergenic 45.0%
ACCAACAGGTCCAAAGGAAA+AGG + chr2.1:33598171-33598190 MS.gene000190:CDS 45.0%
ATTGAACACTGCATCCGTCA+CGG + chr2.1:33596926-33596945 MS.gene000190:CDS 45.0%
CATGACTGTGTTTGCGAAAC+TGG - chr2.1:33596661-33596680 None:intergenic 45.0%
CATGCATCATATCCACATGC+TGG + chr2.1:33597866-33597885 MS.gene000190:CDS 45.0%
CATTGTTAGGATCCTGAGGA+AGG - chr2.1:33597448-33597467 None:intergenic 45.0%
CCAACAGGTCCAAAGGAAAA+GGG + chr2.1:33598172-33598191 MS.gene000190:CDS 45.0%
CCATCATTGTTAGGATCCTG+AGG - chr2.1:33597452-33597471 None:intergenic 45.0%
CCTCAGGATCCTAACAATGA+TGG + chr2.1:33597449-33597468 MS.gene000190:CDS 45.0%
CTGAAGTTAAAGAGCCAGCA+TGG + chr2.1:33597999-33598018 MS.gene000190:CDS 45.0%
CTTGTGTTGTGGTGACAACA+AGG - chr2.1:33597016-33597035 None:intergenic 45.0%
GACACACATGACTTTGTTGG+GGG - chr2.1:33597048-33597067 None:intergenic 45.0%
GACCTGTGGTTTATGCTTCT+CGG + chr2.1:33597837-33597856 MS.gene000190:CDS 45.0%
GCACAAACTTCTTCGAGTGA+AGG + chr2.1:33596540-33596559 MS.gene000190:CDS 45.0%
GCTTGAATGTGTGGTCAATG+GGG - chr2.1:33596512-33596531 None:intergenic 45.0%
GCTTTCCCAGGTCCATTAAA+AGG - chr2.1:33597617-33597636 None:intergenic 45.0%
GGAAGGTTTGTCTTGTGTTG+TGG - chr2.1:33597027-33597046 None:intergenic 45.0%
GGTACCTTCGTTGCTCAAAA+TGG + chr2.1:33597185-33597204 MS.gene000190:CDS 45.0%
TATCCATAAGTCAGTCCCGT+TGG - chr2.1:33596783-33596802 None:intergenic 45.0%
TTGGCTGTAGCTTCCTAACA+TGG - chr2.1:33596808-33596827 None:intergenic 45.0%
! CTCACCATTTTGAGCAACGA+AGG - chr2.1:33597192-33597211 None:intergenic 45.0%
! GGGTTTACCAAAAGAAGTGC+TGG + chr2.1:33598138-33598157 MS.gene000190:CDS 45.0%
! TTTTCCGACACAAAGACCTG+TGG + chr2.1:33597823-33597842 MS.gene000190:CDS 45.0%
!! ATGCTGGCCTTCATCTACTA+GGG + chr2.1:33597882-33597901 MS.gene000190:CDS 45.0%
AGTAGATGAAGGCCAGCATG+TGG - chr2.1:33597881-33597900 None:intergenic 50.0%
CACATGACTTTGTTGGGGGA+AGG - chr2.1:33597044-33597063 None:intergenic 50.0%
CGCCTCAGTGTATGAACCAA+CGG + chr2.1:33596764-33596783 MS.gene000190:CDS 50.0%
GCCTCAGTGTATGAACCAAC+GGG + chr2.1:33596765-33596784 MS.gene000190:CDS 50.0%
GGAGTCTTGATCAACCGTGA+CGG - chr2.1:33596943-33596962 None:intergenic 50.0%
GTGGTTTATGCTTCTCGGCA+TGG + chr2.1:33597842-33597861 MS.gene000190:CDS 50.0%
TGACAACAAGGCCTACAGCA+TGG - chr2.1:33597004-33597023 None:intergenic 50.0%
TGGCTACCAATAGCACCTGA+TGG + chr2.1:33596975-33596994 MS.gene000190:CDS 50.0%
! CCCTTTTCCTTTGGACCTGT+TGG - chr2.1:33598175-33598194 None:intergenic 50.0%
! GAACCAACGGGACTGACTTA+TGG + chr2.1:33596777-33596796 MS.gene000190:CDS 50.0%
! TCCTCTCCCAGCACTTCTTT+TGG - chr2.1:33598148-33598167 None:intergenic 50.0%
! TGCCGAGAAGCATAAACCAC+AGG - chr2.1:33597842-33597861 None:intergenic 50.0%
! TGGTAGCCATCAGGTGCTAT+TGG - chr2.1:33596984-33597003 None:intergenic 50.0%
!! AAAGAAGTGCTGGGAGAGGA+AGG + chr2.1:33598148-33598167 MS.gene000190:CDS 50.0%
!! ACCAAAAGAAGTGCTGGGAG+AGG + chr2.1:33598144-33598163 MS.gene000190:CDS 50.0%
!! CATGCTGGCCTTCATCTACT+AGG + chr2.1:33597881-33597900 MS.gene000190:CDS 50.0%
!! TCCCGTTGGTTCATACACTG+AGG - chr2.1:33596769-33596788 None:intergenic 50.0%
CCTACAGCATGGTAGCCATC+AGG - chr2.1:33596993-33597012 None:intergenic 55.0%
CCTGATGGCTACCATGCTGT+AGG + chr2.1:33596990-33597009 MS.gene000190:CDS 55.0%
GGAAGGACCAACAGGTCCAA+AGG + chr2.1:33598165-33598184 MS.gene000190:CDS 55.0%
! AAAGTTTGGGGCGCCTACGA+AGG + chr2.1:33596723-33596742 MS.gene000190:CDS 55.0%
! AAGGTACCAACGCGAACACC+GGG - chr2.1:33597173-33597192 None:intergenic 55.0%
!! GAAGGTACCAACGCGAACAC+CGG - chr2.1:33597174-33597193 None:intergenic 55.0%
AAAGCTCCCGGTGTTCGCGT+TGG + chr2.1:33597164-33597183 MS.gene000190:CDS 60.0%
ATCGTCCGGTCCACCTTCGT+AGG - chr2.1:33596739-33596758 None:intergenic 60.0%
CTGGGAGAGGAAGGACCAAC+AGG + chr2.1:33598157-33598176 MS.gene000190:CDS 60.0%
GAAGGTGGACCGGACGATCA+AGG + chr2.1:33596741-33596760 MS.gene000190:CDS 60.0%
TGTGGTCAATGGGGCGAGAG+CGG - chr2.1:33596503-33596522 None:intergenic 60.0%
! ACTGAGGCGCCTTGATCGTC+CGG - chr2.1:33596753-33596772 None:intergenic 60.0%
GTGGTCAATGGGGCGAGAGC+GGG - chr2.1:33596502-33596521 None:intergenic 65.0%
! GTTTGGGGCGCCTACGAAGG+TGG + chr2.1:33596726-33596745 MS.gene000190:CDS 65.0%
GGGCGCCTACGAAGGTGGAC+CGG + chr2.1:33596731-33596750 MS.gene000190:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 33596447 33598219 33596447 ID=MS.gene000190
chr2.1 mRNA 33596447 33598219 33596447 ID=MS.gene000190.t1;Parent=MS.gene000190
chr2.1 exon 33596447 33598219 33596447 ID=MS.gene000190.t1.exon1;Parent=MS.gene000190.t1
chr2.1 CDS 33596447 33598219 33596447 ID=cds.MS.gene000190.t1;Parent=MS.gene000190.t1
Gene Sequence

>MS.gene000190

ATGTTTTCATTTGCAGAGAATAAGAATGTCTTCAAGAAAAAGAGACATGTATCCCGCTCTCGCCCCATTGACCACACATTCAAGCTTCCTATTGCACAAACTTCTTCGAGTGAAGGTATTGTTTATGTGATTTCAATTTTCTGTCCTCTTGTAATACATTATAGTGAATGCAGGCTAACTGTAGCAAGTGTTGAATTGCAACATACAGGTACCAGTTTCGCAAACACAGTCATGAATCTAGGTGAGCTGCATGTGATGCAAGCATCAACATTCAGCAAAGTTTGGGGCGCCTACGAAGGTGGACCGGACGATCAAGGCGCCTCAGTGTATGAACCAACGGGACTGACTTATGGATACTCCATGTTAGGAAGCTACAGCCAACCCAACAACAAACCTCTTTTTGGATATGTTCTTGTAGCAAAGGATATTTCAGGAAAGAGCAATGGCACTTTAAAGCCACCTGTTGATTACTCACTAGTATTGAACACTGCATCCGTCACGGTTGATCAAGACTCCCCTTTGTATATTTGGCTACCAATAGCACCTGATGGCTACCATGCTGTAGGCCTTGTTGTCACCACAACACAAGACAAACCTTCCCCCAACAAAGTCATGTGTGTCAGATCAGACCTCACAGAGCAATGTGAGTCTTCTACATGGATTTGGGGATCAAACGACATAAATTTCTATGATGTTAGACCAATCAATAGAGGAACTAAAGCTCCCGGTGTTCGCGTTGGTACCTTCGTTGCTCAAAATGGTGAGAATACCAACCCTCCATCAATTTCATGTTTGAAAAATCTCAATGAAATTTCACAAATCATGCCTAATCTAACACAAATTGAGACAATACTCCACTCTTACTCTCCATTCCTATACTTGCATCCTGATGAAGAATACTTACCTTCCTCTGTCAATTGGTTTTTCTCCAATGGAGCGTTACTATACAAGAAAGGTGAAGAATCAAATCCTGTTGCAATAGTACAAAATGGTACTAACCTTCCTCAGGATCCTAACAATGATGGTGCCTATTGGCTTGACCTACCTGTTGATGATGCAAATAAAGAAAGGGTCAAGCAAGGTAATTTACAAAGTGCAGAATCTTATGTACATGTGAAACCAATGTTTGGAGGAACATTCACTGATATTGCCATGTGGATTTTCTACCCTTTTAATGGACCTGGGAAAGCAAAAGTAGAGTTCATAAATTTGAAGTTAGGGAAAATAGGTGAACATGTTGGTGATTGGGAGCATGTGACATTAAGGGTAAGTAACTTGGATGGTCAGTTATGGCATGTCTATTTCTCTCAACATAATGGTGGTTCATGGCTTGATGCATCCCAACTTGAGTTTCAAACTGATAATAGCAGTTTTGATTTTCCGACACAAAGACCTGTGGTTTATGCTTCTCGGCATGGTCATGCATCATATCCACATGCTGGCCTTCATCTACTAGGGAAAAATGGAGTAGGTGCAAGAGATGATACTGATAAAGGTAGCAATGTTATGGATATGGGAAAATATGTATTGGTTTCTGCAGAGTATTTGGAGTCTGAAGTTAAAGAGCCAGCATGGTTGAATTTTTTTAGGGAGTGGGGTCCACATATTGACTATAGTCTAGATGATGAGTTGAAGAATGTTGAGAAGTTATTGCCTGGGAAATTAAAAGATGTTTTTGAAAAAATTATTATGGGTTTACCAAAAGAAGTGCTGGGAGAGGAAGGACCAACAGGTCCAAAGGAAAAGGGTAATTGGAATGGTGATGAATTTTGA
Protein sequence

>MS.gene000190.t1

MFSFAENKNVFKKKRHVSRSRPIDHTFKLPIAQTSSSEGIVYVISIFCPLVIHYSECRLTVASVELQHTGTSFANTVMNLGELHVMQASTFSKVWGAYEGGPDDQGASVYEPTGLTYGYSMLGSYSQPNNKPLFGYVLVAKDISGKSNGTLKPPVDYSLVLNTASVTVDQDSPLYIWLPIAPDGYHAVGLVVTTTQDKPSPNKVMCVRSDLTEQCESSTWIWGSNDINFYDVRPINRGTKAPGVRVGTFVAQNGENTNPPSISCLKNLNEISQIMPNLTQIETILHSYSPFLYLHPDEEYLPSSVNWFFSNGALLYKKGEESNPVAIVQNGTNLPQDPNNDGAYWLDLPVDDANKERVKQGNLQSAESYVHVKPMFGGTFTDIAMWIFYPFNGPGKAKVEFINLKLGKIGEHVGDWEHVTLRVSNLDGQLWHVYFSQHNGGSWLDASQLEFQTDNSSFDFPTQRPVVYASRHGHASYPHAGLHLLGKNGVGARDDTDKGSNVMDMGKYVLVSAEYLESEVKEPAWLNFFREWGPHIDYSLDDELKNVEKLLPGKLKDVFEKIIMGLPKEVLGEEGPTGPKEKGNWNGDEF