AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000193.t1 MTR_2g062430 85.714 553 22 2 1 496 1 553 0.0 958
MS.gene000193.t1 MTR_2g062410 70.620 548 97 4 13 496 39 586 0.0 783
MS.gene000193.t1 MTR_2g062370 66.234 539 116 3 24 496 52 590 0.0 732
MS.gene000193.t1 MTR_4g131710 63.949 552 131 4 13 496 35 586 0.0 704
MS.gene000193.t1 MTR_2g062380 65.326 522 114 4 41 496 40 560 0.0 689
MS.gene000193.t1 MTR_2g067640 55.678 546 160 10 19 496 10 541 0.0 570
MS.gene000193.t1 MTR_2g062340 50.820 549 158 13 18 495 9 516 5.95e-169 487
MS.gene000193.t1 MTR_1g080370 42.647 544 227 9 29 495 23 558 2.06e-144 426
MS.gene000193.t1 MTR_1g080410 42.647 544 227 9 29 495 23 558 2.06e-144 426
MS.gene000193.t1 MTR_4g077620 41.506 571 254 11 1 495 1 567 7.81e-140 414
MS.gene000193.t1 MTR_5g007860 41.774 541 229 8 38 495 68 605 8.77e-140 416
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000193.t1 AT2G44260 53.321 557 193 6 1 495 1 552 0.0 588
MS.gene000193.t1 AT2G44260 50.767 587 192 7 1 495 1 582 0.0 574
MS.gene000193.t1 AT2G44230 51.577 539 188 8 22 496 13 542 0.0 532
MS.gene000193.t1 AT3G01870 47.114 537 217 9 22 495 51 583 1.15e-163 476
MS.gene000193.t1 AT3G01880 46.168 561 228 12 5 495 33 589 4.13e-157 459
MS.gene000193.t1 AT5G18490 41.002 539 236 9 29 496 25 552 3.57e-139 412
MS.gene000193.t1 AT3G04350 39.560 546 247 9 29 496 26 566 2.63e-132 395
MS.gene000193.t1 AT1G04090 39.855 552 242 13 29 495 24 570 9.03e-130 389
MS.gene000193.t1 AT5G43950 39.852 542 250 11 29 495 24 564 2.10e-129 387
MS.gene000193.t1 AT3G04350 39.513 534 240 9 41 496 16 544 4.77e-129 386

Find 118 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGTTCATAAATGTTAAAT+TGG 0.173007 2.1:-33550332 MS.gene000193:CDS
GCTCTTCCAGGTCCATTAAA+AGG 0.179021 2.1:+33550361 None:intergenic
AAGATACCACATGTTATATT+TGG 0.191626 2.1:-33551020 MS.gene000193:CDS
TATCAACATTTAACAAAGTT+TGG 0.199497 2.1:-33551269 MS.gene000193:CDS
GCGAGTCTTCTTCGTGGATT+TGG 0.208690 2.1:-33550885 MS.gene000193:CDS
ATTGAACACATCAACTATTA+AGG 0.251355 2.1:-33551049 MS.gene000193:CDS
CGAGTCTTCTTCGTGGATTT+GGG 0.254463 2.1:-33550884 MS.gene000193:CDS
GTCCAAAGCTGAAGAATAAT+TGG 0.267074 2.1:-33549823 MS.gene000193:CDS
GGCACCTTTGTTGCACAAAA+TGG 0.283924 2.1:-33550790 MS.gene000193:CDS
TCCTCTCCCAGCACTTCTTC+TGG 0.294781 2.1:+33549857 None:intergenic
CTCCAATTATTCTTCAGCTT+TGG 0.329586 2.1:+33549821 None:intergenic
AATGTTAAATTGGGGAAAAT+AGG 0.333070 2.1:-33550322 MS.gene000193:CDS
ATCAACATTTAACAAAGTTT+GGG 0.335558 2.1:-33551268 MS.gene000193:CDS
CCTAATGATTTGTAGCCTTC+AGG 0.343612 2.1:+33550985 None:intergenic
CCAAAGAGAGGCTTATTGTT+TGG 0.347453 2.1:+33551150 None:intergenic
ATACCTGGTGGCCAAGCATT+TGG 0.352008 2.1:+33551638 None:intergenic
GGCCAAGCATTTGGAGCAAT+TGG 0.358291 2.1:+33551647 None:intergenic
AAGTTCATAAATGTTAAATT+GGG 0.368198 2.1:-33550331 MS.gene000193:CDS
GCATCAATTTGTACTAGATT+AGG 0.374766 2.1:+33550703 None:intergenic
CCAAACAATAAGCCTCTCTT+TGG 0.390475 2.1:-33551150 MS.gene000193:CDS
ATTATCTGGTCCACCATCAT+AGG 0.395820 2.1:+33551242 None:intergenic
AATACAAGTGTATAATCAAT+TGG 0.395907 2.1:+33551069 None:intergenic
AGCAATTGGAAGCTTGAATA+TGG 0.401497 2.1:+33551661 None:intergenic
TTGTAGCCTTCAGGTGCTAT+TGG 0.401546 2.1:+33550994 None:intergenic
ATTGTGGCACCTTGATTATC+TGG 0.402581 2.1:+33551228 None:intergenic
ACTTACCTTCCTCTGTCAAT+TGG 0.417119 2.1:-33550630 MS.gene000193:CDS
ATTGGGAGCATGTGACCTTA+AGG 0.423436 2.1:-33550285 MS.gene000193:CDS
AAAATATCCTTTAGTTTCCA+TGG 0.425791 2.1:+33549902 None:intergenic
CCTCAAGGATTCTCCATGTT+AGG 0.427209 2.1:-33551186 MS.gene000193:CDS
CTAACAATGATGGTACCTAT+TGG 0.429733 2.1:-33550516 MS.gene000193:CDS
ATCTAAATTACTCACCCTTA+AGG 0.430906 2.1:+33550270 None:intergenic
TTCTTCAGCTTTGGACCTGT+GGG 0.433050 2.1:+33549830 None:intergenic
CTTGTATAGTAATGCTCCAT+TGG 0.433338 2.1:+33550600 None:intergenic
CAATAGGTACCATCATTGTT+AGG 0.437937 2.1:+33550517 None:intergenic
CCTGTTGCAATAGCACAAAA+TGG 0.439644 2.1:-33550559 MS.gene000193:CDS
GAAGGTAAGTATTCTTCATC+AGG 0.444599 2.1:+33550643 None:intergenic
TTCCAATTGCTCCAAATGCT+TGG 0.445747 2.1:-33551649 MS.gene000193:CDS
GAGTTTACCAGAAGAAGTGC+TGG 0.446564 2.1:-33549864 MS.gene000193:CDS
CCTGAAGGCTACAAATCATT+AGG 0.447915 2.1:-33550985 MS.gene000193:CDS
CTCTTCCAGGTCCATTAAAA+GGG 0.448662 2.1:+33550362 None:intergenic
AGATGAGGAGTTGAGGAAGG+TGG 0.450535 2.1:-33549936 MS.gene000193:CDS
GCTCCAAATGCTTGGCCACC+AGG 0.451485 2.1:-33551641 MS.gene000193:intron
ATTCTTCAGCTTTGGACCTG+TGG 0.451562 2.1:+33549829 None:intergenic
CATGAAGGACTAGTTCTTCA+AGG 0.455081 2.1:-33550121 MS.gene000193:CDS
TAGGTGAGCATGTTGGTGAT+TGG 0.460021 2.1:-33550303 MS.gene000193:CDS
AGAAGGTAGGTCAAGCCAAT+AGG 0.478817 2.1:+33550501 None:intergenic
AAAGTTTGGGCCACCTATGA+TGG 0.480692 2.1:-33551255 MS.gene000193:CDS
AGAGAGGCTTATTGTTTGGT+TGG 0.485270 2.1:+33551154 None:intergenic
ACCTTTGTTGCACAAAATGG+TGG 0.487087 2.1:-33550787 MS.gene000193:CDS
GCAAATGGAATTATTGATGT+AGG 0.495761 2.1:-33551312 MS.gene000193:CDS
GCCACAATATTTGAACCAAC+AGG 0.497066 2.1:-33551213 MS.gene000193:CDS
GAAGAAGTGCTGGGAGAGGA+AGG 0.504490 2.1:-33549854 MS.gene000193:CDS
TCAGGATGCAAGTACATCAA+TGG 0.505882 2.1:+33550661 None:intergenic
GTGAAGGTTCCTCCAAGCAT+TGG 0.506638 2.1:+33550409 None:intergenic
ACCAATGCGAGTCTTCTTCG+TGG 0.508222 2.1:-33550891 MS.gene000193:CDS
AGGTGAGCATGTTGGTGATT+GGG 0.511142 2.1:-33550302 MS.gene000193:CDS
CTGGGAGAGGAAGGACCCAC+AGG 0.511477 2.1:-33549845 MS.gene000193:CDS
ACCTGTTGGTTCAAATATTG+TGG 0.520433 2.1:+33551212 None:intergenic
AAGCTTGAATATGGTGTCAA+TGG 0.520587 2.1:+33551670 None:intergenic
TATAGATGAGGAGTTGAGGA+AGG 0.523513 2.1:-33549939 MS.gene000193:CDS
GATATTGCCATGGAAACTAA+AGG 0.528394 2.1:-33549909 MS.gene000193:CDS
TGGATATGTTCTTGTGGCAA+AGG 0.530949 2.1:-33551130 MS.gene000193:CDS
GAATATGGTGTCAATGGGAC+GGG 0.533624 2.1:+33551676 None:intergenic
CCTCAGGATCCTAACAATGA+TGG 0.535568 2.1:-33550526 MS.gene000193:CDS
TGTTTGCATCATCAGAAGGT+AGG 0.539120 2.1:+33550488 None:intergenic
ACCAGAAGAAGTGCTGGGAG+AGG 0.544118 2.1:-33549858 MS.gene000193:CDS
AAATGGTACTAACCTTCCTC+AGG 0.545862 2.1:-33550542 MS.gene000193:CDS
TCTCTTTGGATATGTTCTTG+TGG 0.546851 2.1:-33551136 MS.gene000193:CDS
AGTTCATAAATGTTAAATTG+GGG 0.549300 2.1:-33550330 MS.gene000193:CDS
ATAATATAGATGAGGAGTTG+AGG 0.551941 2.1:-33549943 MS.gene000193:CDS
CTTGTGTTGTGGTGACAACA+TGG 0.561956 2.1:+33550962 None:intergenic
TCTTTGTTTGCATCATCAGA+AGG 0.565098 2.1:+33550484 None:intergenic
CCTTTGTTGCACAAAATGGT+GGG 0.565779 2.1:-33550786 MS.gene000193:CDS
TCCACGAAGAAGACTCGCAT+TGG 0.565993 2.1:+33550890 None:intergenic
TTCAAACATGAAATAGATGG+AGG 0.566116 2.1:+33550754 None:intergenic
GGAGCATTACTATACAAGAA+AGG 0.567167 2.1:-33550595 MS.gene000193:CDS
AGCTTGAATATGGTGTCAAT+GGG 0.568653 2.1:+33551671 None:intergenic
CATTGTTAGGATCCTGAGGA+AGG 0.572703 2.1:+33550530 None:intergenic
CCACATGGCAATATCTGTGA+AGG 0.573443 2.1:+33550393 None:intergenic
AATCCTTGAGGTATACCTGT+TGG 0.581735 2.1:+33551198 None:intergenic
TGAATATGGTGTCAATGGGA+CGG 0.584979 2.1:+33551675 None:intergenic
CTGATGATGCAAACAAAGAG+AGG 0.585320 2.1:-33550480 MS.gene000193:CDS
GATGGTGGACCAGATAATCA+AGG 0.585776 2.1:-33551237 MS.gene000193:CDS
GAGTCTTCTTCGTGGATTTG+GGG 0.588057 2.1:-33550883 MS.gene000193:CDS
TGGTGTCAATGGGACGGGAA+CGG 0.590732 2.1:+33551681 None:intergenic
TTGGGAGCATGTGACCTTAA+GGG 0.592040 2.1:-33550284 MS.gene000193:intron
ATGAAGGACTAGTTCTTCAA+GGG 0.592808 2.1:-33550120 MS.gene000193:CDS
GTACATGTGAAACCAATGCT+TGG 0.596217 2.1:-33550421 MS.gene000193:CDS
GATAGAGGTAGTCAAGCGGC+TGG 0.596765 2.1:-33550823 MS.gene000193:CDS
GCTTGACTACCTCTATCGAT+TGG 0.598997 2.1:+33550829 None:intergenic
ATCTGGTCCACCATCATAGG+TGG 0.604053 2.1:+33551245 None:intergenic
TTGGCTGTAGCTACCTAACA+TGG 0.605814 2.1:+33551173 None:intergenic
TTCCTAAAATAGTGCAACCA+AGG 0.605969 2.1:+33549986 None:intergenic
GAACCAACAGGTATACCTCA+AGG 0.612915 2.1:-33551201 MS.gene000193:CDS
CATGCATCATATCCACATGA+AGG 0.613738 2.1:-33550136 MS.gene000193:CDS
TGAAGGTTTATCTTGTGTTG+TGG 0.614581 2.1:+33550951 None:intergenic
CCATCATTGTTAGGATCCTG+AGG 0.614872 2.1:+33550526 None:intergenic
AATGGAATTATTGATGTAGG+TGG 0.617932 2.1:-33551309 MS.gene000193:CDS
TGATGATGCAAACAAAGAGA+GGG 0.621716 2.1:-33550479 MS.gene000193:CDS
AGTTTACCAGAAGAAGTGCT+GGG 0.622675 2.1:-33549863 MS.gene000193:CDS
ATTATCTACACAACAACATG+AGG 0.622890 2.1:+33551724 None:intergenic
AAGCTGAAGAATAATTGGAG+TGG 0.625543 2.1:-33549818 MS.gene000193:CDS
GTTTGGGCCACCTATGATGG+TGG 0.627591 2.1:-33551252 MS.gene000193:CDS
GATGTTAGACCAATCGATAG+AGG 0.629250 2.1:-33550838 MS.gene000193:CDS
TGGTTACCAATAGCACCTGA+AGG 0.631904 2.1:-33551000 MS.gene000193:CDS
CAAGCGGCTGGTGTTCACAT+CGG 0.633068 2.1:-33550811 MS.gene000193:CDS
TGGTAACCAAATATAACATG+TGG 0.648559 2.1:+33551014 None:intergenic
CATGTGAAACCAATGCTTGG+AGG 0.650721 2.1:-33550418 MS.gene000193:CDS
CTAAAATAGTGCAACCAAGG+TGG 0.652677 2.1:+33549989 None:intergenic
AATTGATTATAATATAGATG+AGG 0.654441 2.1:-33549951 MS.gene000193:CDS
ACAAGAACATATCCAAAGAG+AGG 0.658317 2.1:+33551138 None:intergenic
AGGTGGAGAAGATATTGCCA+TGG 0.662411 2.1:-33549919 MS.gene000193:CDS
GGGAAAATAGGTGAGCATGT+TGG 0.662816 2.1:-33550310 MS.gene000193:CDS
TGAAGAACTAGTCCTTCATG+TGG 0.664200 2.1:+33550124 None:intergenic
CCTAACATGGAGAATCCTTG+AGG 0.675121 2.1:+33551186 None:intergenic
AAGACTCGCATTGGTCTGTG+AGG 0.675872 2.1:+33550899 None:intergenic
AATCGATAGAGGTAGTCAAG+CGG 0.717297 2.1:-33550827 MS.gene000193:CDS
CCTTCACAGATATTGCCATG+TGG 0.739729 2.1:-33550393 MS.gene000193:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATATTATAATCAATTTT+TGG + chr2.1:33551592-33551611 None:intergenic 10.0%
!! AAAGTTCATAAATGTTAAAT+TGG - chr2.1:33551213-33551232 MS.gene000193:CDS 15.0%
!! AAGTTCATAAATGTTAAATT+GGG - chr2.1:33551214-33551233 MS.gene000193:CDS 15.0%
!! AATATTACACACAAATTAAA+GGG - chr2.1:33550029-33550048 MS.gene000193:intron 15.0%
!! AATTGATTATAATATAGATG+AGG - chr2.1:33551594-33551613 MS.gene000193:intron 15.0%
!! TTTATAGTAACTATTTAGTA+TGG - chr2.1:33550091-33550110 MS.gene000193:intron 15.0%
!! AATACAAGTGTATAATCAAT+TGG + chr2.1:33550479-33550498 None:intergenic 20.0%
!! AGTTCATAAATGTTAAATTG+GGG - chr2.1:33551215-33551234 MS.gene000193:CDS 20.0%
!! ATCAACATTTAACAAAGTTT+GGG - chr2.1:33550277-33550296 MS.gene000193:intron 20.0%
!! GAATATTACACACAAATTAA+AGG - chr2.1:33550028-33550047 MS.gene000193:intron 20.0%
!! TATCAACATTTAACAAAGTT+TGG - chr2.1:33550276-33550295 MS.gene000193:intron 20.0%
!!! AAAATGGATTTTTTTTTCCA+AGG - chr2.1:33549995-33550014 MS.gene000193:intron 20.0%
!!! CACTAGTTTAAGTATATTTT+TGG + chr2.1:33550120-33550139 None:intergenic 20.0%
!!! TTTTTCAAACATGAAATAGA+TGG + chr2.1:33550797-33550816 None:intergenic 20.0%
! AAATATACTTAAACTAGTGC+TGG - chr2.1:33550121-33550140 MS.gene000193:CDS 25.0%
! AAGATACCACATGTTATATT+TGG - chr2.1:33550525-33550544 MS.gene000193:CDS 25.0%
! AATGTTAAATTGGGGAAAAT+AGG - chr2.1:33551223-33551242 MS.gene000193:CDS 25.0%
! ACAATCACAAAATTAATACC+TGG + chr2.1:33549925-33549944 None:intergenic 25.0%
! ATGATTATAACTAGTACTGT+TGG - chr2.1:33550185-33550204 MS.gene000193:CDS 25.0%
! ATTGAACACATCAACTATTA+AGG - chr2.1:33550496-33550515 MS.gene000193:CDS 25.0%
! GTAATTTAGATGGTCAATTA+TGG - chr2.1:33551287-33551306 MS.gene000193:CDS 25.0%
! TATTTCTCACAACATAGTAA+AGG - chr2.1:33551316-33551335 MS.gene000193:CDS 25.0%
! TGCAATGAAGAATAAAAAAC+TGG + chr2.1:33551385-33551404 None:intergenic 25.0%
!! AAAATATCCTTTAGTTTCCA+TGG + chr2.1:33551646-33551665 None:intergenic 25.0%
!! ATTGATTTCTGCTGATTATT+TGG - chr2.1:33551516-33551535 MS.gene000193:intron 25.0%
!! TTGATTTCTGCTGATTATTT+GGG - chr2.1:33551517-33551536 MS.gene000193:intron 25.0%
!!! AATATTCATTTTAGTCTCCT+TGG + chr2.1:33550015-33550034 None:intergenic 25.0%
!!! ATGATTTTTTCCTCTACATA+AGG - chr2.1:33549969-33549988 MS.gene000193:CDS 25.0%
!!! GTTTTTTATTCTTCATTGCA+TGG - chr2.1:33551385-33551404 MS.gene000193:intron 25.0%
!!! TGGATTTTTTACCCTTTTAA+TGG - chr2.1:33551172-33551191 MS.gene000193:CDS 25.0%
AAAAGGGTAAAAAATCCACA+TGG + chr2.1:33551170-33551189 None:intergenic 30.0%
AACAAAGAGAGGGTAAAAAA+AGG - chr2.1:33551076-33551095 MS.gene000193:CDS 30.0%
AATGGAATTATTGATGTAGG+TGG - chr2.1:33550236-33550255 MS.gene000193:intron 30.0%
ACTGTTGGATTGTTACATAT+AGG - chr2.1:33550200-33550219 MS.gene000193:intron 30.0%
ATAATATAGATGAGGAGTTG+AGG - chr2.1:33551602-33551621 MS.gene000193:intron 30.0%
ATCACAAAATTAATACCTGG+TGG + chr2.1:33549922-33549941 None:intergenic 30.0%
ATCTAAATTACTCACCCTTA+AGG + chr2.1:33551278-33551297 None:intergenic 30.0%
ATTATCTACACAACAACATG+AGG + chr2.1:33549824-33549843 None:intergenic 30.0%
ATTGTCAATTTCACACTTAC+GGG - chr2.1:33549940-33549959 MS.gene000193:CDS 30.0%
GATTGTCAATTTCACACTTA+CGG - chr2.1:33549939-33549958 MS.gene000193:CDS 30.0%
GCAAATGGAATTATTGATGT+AGG - chr2.1:33550233-33550252 MS.gene000193:intron 30.0%
GCATCAATTTGTACTAGATT+AGG + chr2.1:33550845-33550864 None:intergenic 30.0%
TGGTAACCAAATATAACATG+TGG + chr2.1:33550534-33550553 None:intergenic 30.0%
TTAAGGGTGAGTAATTTAGA+TGG - chr2.1:33551277-33551296 MS.gene000193:CDS 30.0%
TTCAAACATGAAATAGATGG+AGG + chr2.1:33550794-33550813 None:intergenic 30.0%
! TGATTTCTGCTGATTATTTG+GGG - chr2.1:33551518-33551537 MS.gene000193:intron 30.0%
! TGCTAAGAGTGAAATGATTT+TGG - chr2.1:33551477-33551496 MS.gene000193:intron 30.0%
!! GTTGCACTATTTTAGGAAAT+GGG - chr2.1:33551564-33551583 MS.gene000193:intron 30.0%
!! TTGCACTATTTTAGGAAATG+GGG - chr2.1:33551565-33551584 MS.gene000193:intron 30.0%
AAAAACCAATTGACAGAGGA+AGG + chr2.1:33550923-33550942 None:intergenic 35.0%
AAGCTGAAGAATAATTGGAG+TGG - chr2.1:33551727-33551746 MS.gene000193:CDS 35.0%
ACAAGAACATATCCAAAGAG+AGG + chr2.1:33550410-33550429 None:intergenic 35.0%
AGCAATTGGAAGCTTGAATA+TGG + chr2.1:33549887-33549906 None:intergenic 35.0%
CAATAGGTACCATCATTGTT+AGG + chr2.1:33551031-33551050 None:intergenic 35.0%
CACAACATAGTAAAGGTGAA+TGG - chr2.1:33551323-33551342 MS.gene000193:CDS 35.0%
CCTCTACATAAGGACTAAAA+TGG - chr2.1:33549979-33549998 MS.gene000193:CDS 35.0%
CTAACAATGATGGTACCTAT+TGG - chr2.1:33551029-33551048 MS.gene000193:CDS 35.0%
CTCCAATTATTCTTCAGCTT+TGG + chr2.1:33551727-33551746 None:intergenic 35.0%
CTTCAAGGGAAAAATGGAAT+AGG - chr2.1:33551439-33551458 MS.gene000193:intron 35.0%
CTTGTATAGTAATGCTCCAT+TGG + chr2.1:33550948-33550967 None:intergenic 35.0%
GAAGGTAAGTATTCTTCATC+AGG + chr2.1:33550905-33550924 None:intergenic 35.0%
GATATTGCCATGGAAACTAA+AGG - chr2.1:33551636-33551655 MS.gene000193:intron 35.0%
GGATTGTTACATATAGGTAG+TGG - chr2.1:33550206-33550225 MS.gene000193:intron 35.0%
GTCCAAAGCTGAAGAATAAT+TGG - chr2.1:33551722-33551741 MS.gene000193:CDS 35.0%
TCTCTTTGGATATGTTCTTG+TGG - chr2.1:33550409-33550428 MS.gene000193:CDS 35.0%
TGAAGGTTTATCTTGTGTTG+TGG + chr2.1:33550597-33550616 None:intergenic 35.0%
TTCCTAAAATAGTGCAACCA+AGG + chr2.1:33551562-33551581 None:intergenic 35.0%
! AAGCTTGAATATGGTGTCAA+TGG + chr2.1:33549878-33549897 None:intergenic 35.0%
! AGCTTGAATATGGTGTCAAT+GGG + chr2.1:33549877-33549896 None:intergenic 35.0%
! CACATGATTTTGTCAAGTGA+AGG + chr2.1:33550614-33550633 None:intergenic 35.0%
! CTAGTTCTTCAAGGGAAAAA+TGG - chr2.1:33551433-33551452 MS.gene000193:intron 35.0%
! GGAGCATTACTATACAAGAA+AGG - chr2.1:33550950-33550969 MS.gene000193:CDS 35.0%
! TATACTTAAACTAGTGCTGG+TGG - chr2.1:33550124-33550143 MS.gene000193:CDS 35.0%
! TCTTTGTTTGCATCATCAGA+AGG + chr2.1:33551064-33551083 None:intergenic 35.0%
! TGATGATGCAAACAAAGAGA+GGG - chr2.1:33551066-33551085 MS.gene000193:CDS 35.0%
!! AACTTTACTTTTGCTCTTCC+AGG + chr2.1:33551199-33551218 None:intergenic 35.0%
!! ACCTGTTGGTTCAAATATTG+TGG + chr2.1:33550336-33550355 None:intergenic 35.0%
!! ATACTTAAACTAGTGCTGGT+GGG - chr2.1:33550125-33550144 MS.gene000193:CDS 35.0%
!! ATAGGTAGTGGTTTTGCAAA+TGG - chr2.1:33550218-33550237 MS.gene000193:intron 35.0%
!! ATGAAGGACTAGTTCTTCAA+GGG - chr2.1:33551425-33551444 MS.gene000193:intron 35.0%
!! CCATTTTAGTCCTTATGTAG+AGG + chr2.1:33549982-33550001 None:intergenic 35.0%
!! GGTTGCACTATTTTAGGAAA+TGG - chr2.1:33551563-33551582 MS.gene000193:intron 35.0%
!! TTTTACCCTTTTAATGGACC+TGG - chr2.1:33551178-33551197 MS.gene000193:CDS 35.0%
!!! GTCAATTGGTTTTTCTCCAA+TGG - chr2.1:33550929-33550948 MS.gene000193:CDS 35.0%
AAATGGTACTAACCTTCCTC+AGG - chr2.1:33551003-33551022 MS.gene000193:CDS 40.0%
AATCCTTGAGGTATACCTGT+TGG + chr2.1:33550350-33550369 None:intergenic 40.0%
AATCGATAGAGGTAGTCAAG+CGG - chr2.1:33550718-33550737 MS.gene000193:CDS 40.0%
ACCTTTGTTGCACAAAATGG+TGG - chr2.1:33550758-33550777 MS.gene000193:CDS 40.0%
ACTTACCTTCCTCTGTCAAT+TGG - chr2.1:33550915-33550934 MS.gene000193:CDS 40.0%
AGAGAGGCTTATTGTTTGGT+TGG + chr2.1:33550394-33550413 None:intergenic 40.0%
ATTATCTGGTCCACCATCAT+AGG + chr2.1:33550306-33550325 None:intergenic 40.0%
ATTGTGGCACCTTGATTATC+TGG + chr2.1:33550320-33550339 None:intergenic 40.0%
CATGCATCATATCCACATGA+AGG - chr2.1:33551409-33551428 MS.gene000193:intron 40.0%
CCAAACAATAAGCCTCTCTT+TGG - chr2.1:33550395-33550414 MS.gene000193:CDS 40.0%
CCAAAGAGAGGCTTATTGTT+TGG + chr2.1:33550398-33550417 None:intergenic 40.0%
CCTGAAGGCTACAAATCATT+AGG - chr2.1:33550560-33550579 MS.gene000193:CDS 40.0%
CCTGTTGCAATAGCACAAAA+TGG - chr2.1:33550986-33551005 MS.gene000193:CDS 40.0%
CCTTTGTTGCACAAAATGGT+GGG - chr2.1:33550759-33550778 MS.gene000193:CDS 40.0%
CTAAAATAGTGCAACCAAGG+TGG + chr2.1:33551559-33551578 None:intergenic 40.0%
CTCTTCCAGGTCCATTAAAA+GGG + chr2.1:33551186-33551205 None:intergenic 40.0%
GATGTTAGACCAATCGATAG+AGG - chr2.1:33550707-33550726 MS.gene000193:CDS 40.0%
GCCACAATATTTGAACCAAC+AGG - chr2.1:33550332-33550351 MS.gene000193:CDS 40.0%
GGAGAAAAACCAATTGACAG+AGG + chr2.1:33550927-33550946 None:intergenic 40.0%
GTACATGTGAAACCAATGCT+TGG - chr2.1:33551124-33551143 MS.gene000193:CDS 40.0%
TATAGATGAGGAGTTGAGGA+AGG - chr2.1:33551606-33551625 MS.gene000193:intron 40.0%
TCAGGATGCAAGTACATCAA+TGG + chr2.1:33550887-33550906 None:intergenic 40.0%
TGAAGAACTAGTCCTTCATG+TGG + chr2.1:33551424-33551443 None:intergenic 40.0%
TGGATATGTTCTTGTGGCAA+AGG - chr2.1:33550415-33550434 MS.gene000193:CDS 40.0%
TGTTTGCATCATCAGAAGGT+AGG + chr2.1:33551060-33551079 None:intergenic 40.0%
TTCCAATTGCTCCAAATGCT+TGG - chr2.1:33549896-33549915 MS.gene000193:CDS 40.0%
! CACCTTGGTTGCACTATTTT+AGG - chr2.1:33551557-33551576 MS.gene000193:intron 40.0%
! CCTAATGATTTGTAGCCTTC+AGG + chr2.1:33550563-33550582 None:intergenic 40.0%
! CTGATGATGCAAACAAAGAG+AGG - chr2.1:33551065-33551084 MS.gene000193:CDS 40.0%
! TGAATATGGTGTCAATGGGA+CGG + chr2.1:33549873-33549892 None:intergenic 40.0%
!! AGTTTACCAGAAGAAGTGCT+GGG - chr2.1:33551682-33551701 MS.gene000193:CDS 40.0%
!! CATGAAGGACTAGTTCTTCA+AGG - chr2.1:33551424-33551443 MS.gene000193:intron 40.0%
!! CCATTTTGTGCTATTGCAAC+AGG + chr2.1:33550989-33551008 None:intergenic 40.0%
!! CTTTTGTTATAGAGCCACCT+TGG - chr2.1:33551542-33551561 MS.gene000193:intron 40.0%
AAAGTTTGGGCCACCTATGA+TGG - chr2.1:33550290-33550309 MS.gene000193:CDS 45.0%
AGAAGGTAGGTCAAGCCAAT+AGG + chr2.1:33551047-33551066 None:intergenic 45.0%
AGGTGGAGAAGATATTGCCA+TGG - chr2.1:33551626-33551645 MS.gene000193:intron 45.0%
CATGTGAAACCAATGCTTGG+AGG - chr2.1:33551127-33551146 MS.gene000193:CDS 45.0%
CATTGTTAGGATCCTGAGGA+AGG + chr2.1:33551018-33551037 None:intergenic 45.0%
CCACATGGCAATATCTGTGA+AGG + chr2.1:33551155-33551174 None:intergenic 45.0%
CCATCATTGTTAGGATCCTG+AGG + chr2.1:33551022-33551041 None:intergenic 45.0%
CCTAACATGGAGAATCCTTG+AGG + chr2.1:33550362-33550381 None:intergenic 45.0%
CCTCAAGGATTCTCCATGTT+AGG - chr2.1:33550359-33550378 MS.gene000193:CDS 45.0%
CCTCAGGATCCTAACAATGA+TGG - chr2.1:33551019-33551038 MS.gene000193:CDS 45.0%
CCTTCACAGATATTGCCATG+TGG - chr2.1:33551152-33551171 MS.gene000193:CDS 45.0%
CGAGTCTTCTTCGTGGATTT+GGG - chr2.1:33550661-33550680 MS.gene000193:CDS 45.0%
CTTGTGTTGTGGTGACAACA+TGG + chr2.1:33550586-33550605 None:intergenic 45.0%
GAACCAACAGGTATACCTCA+AGG - chr2.1:33550344-33550363 MS.gene000193:CDS 45.0%
GAGTCTTCTTCGTGGATTTG+GGG - chr2.1:33550662-33550681 MS.gene000193:CDS 45.0%
GCTCTTCCAGGTCCATTAAA+AGG + chr2.1:33551187-33551206 None:intergenic 45.0%
GCTTGACTACCTCTATCGAT+TGG + chr2.1:33550719-33550738 None:intergenic 45.0%
GGCACCTTTGTTGCACAAAA+TGG - chr2.1:33550755-33550774 MS.gene000193:CDS 45.0%
GGGAAAATAGGTGAGCATGT+TGG - chr2.1:33551235-33551254 MS.gene000193:CDS 45.0%
TGGTTACCAATAGCACCTGA+AGG - chr2.1:33550545-33550564 MS.gene000193:CDS 45.0%
TTGGCTGTAGCTACCTAACA+TGG + chr2.1:33550375-33550394 None:intergenic 45.0%
! ATTGGGAGCATGTGACCTTA+AGG - chr2.1:33551260-33551279 MS.gene000193:CDS 45.0%
! CCCACCATTTTGTGCAACAA+AGG + chr2.1:33550762-33550781 None:intergenic 45.0%
! GAATATGGTGTCAATGGGAC+GGG + chr2.1:33549872-33549891 None:intergenic 45.0%
! GAGTTTACCAGAAGAAGTGC+TGG - chr2.1:33551681-33551700 MS.gene000193:CDS 45.0%
! GCTGGTGGGATTTCAGTTTT+AGG - chr2.1:33550139-33550158 MS.gene000193:CDS 45.0%
! TTGGGAGCATGTGACCTTAA+GGG - chr2.1:33551261-33551280 MS.gene000193:CDS 45.0%
! TTGTAGCCTTCAGGTGCTAT+TGG + chr2.1:33550554-33550573 None:intergenic 45.0%
!! AGGTGAGCATGTTGGTGATT+GGG - chr2.1:33551243-33551262 MS.gene000193:CDS 45.0%
!! ATTCTTCAGCTTTGGACCTG+TGG + chr2.1:33551719-33551738 None:intergenic 45.0%
!! GATGGTGGACCAGATAATCA+AGG - chr2.1:33550308-33550327 MS.gene000193:CDS 45.0%
!! TAGGTGAGCATGTTGGTGAT+TGG - chr2.1:33551242-33551261 MS.gene000193:CDS 45.0%
!! TTCTTCAGCTTTGGACCTGT+GGG + chr2.1:33551718-33551737 None:intergenic 45.0%
!!! ATATTTTTGAAAATATTATT+AGG - chr2.1:33551659-33551678 MS.gene000193:CDS 5.0%
ACCAATGCGAGTCTTCTTCG+TGG - chr2.1:33550654-33550673 MS.gene000193:CDS 50.0%
AGATGAGGAGTTGAGGAAGG+TGG - chr2.1:33551609-33551628 MS.gene000193:intron 50.0%
ATACCTGGTGGCCAAGCATT+TGG + chr2.1:33549910-33549929 None:intergenic 50.0%
ATCTGGTCCACCATCATAGG+TGG + chr2.1:33550303-33550322 None:intergenic 50.0%
GCGAGTCTTCTTCGTGGATT+TGG - chr2.1:33550660-33550679 MS.gene000193:CDS 50.0%
GTGAAGGTTCCTCCAAGCAT+TGG + chr2.1:33551139-33551158 None:intergenic 50.0%
TCCACGAAGAAGACTCGCAT+TGG + chr2.1:33550658-33550677 None:intergenic 50.0%
! AAGACTCGCATTGGTCTGTG+AGG + chr2.1:33550649-33550668 None:intergenic 50.0%
! GGCCAAGCATTTGGAGCAAT+TGG + chr2.1:33549901-33549920 None:intergenic 50.0%
CAAGCGGCTGGTGTTCACAT+CGG - chr2.1:33550734-33550753 MS.gene000193:CDS 55.0%
GATAGAGGTAGTCAAGCGGC+TGG - chr2.1:33550722-33550741 MS.gene000193:CDS 55.0%
GTTTGGGCCACCTATGATGG+TGG - chr2.1:33550293-33550312 MS.gene000193:CDS 55.0%
TCCTCTCCCAGCACTTCTTC+TGG + chr2.1:33551691-33551710 None:intergenic 55.0%
! TGGTGTCAATGGGACGGGAA+CGG + chr2.1:33549867-33549886 None:intergenic 55.0%
!! ACCAGAAGAAGTGCTGGGAG+AGG - chr2.1:33551687-33551706 MS.gene000193:CDS 55.0%
!! GAAGAAGTGCTGGGAGAGGA+AGG - chr2.1:33551691-33551710 MS.gene000193:CDS 55.0%
GCTCCAAATGCTTGGCCACC+AGG - chr2.1:33549904-33549923 MS.gene000193:CDS 60.0%
CTGGGAGAGGAAGGACCCAC+AGG - chr2.1:33551700-33551719 MS.gene000193:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 33549805 33551762 33549805 ID=MS.gene000193
chr2.1 mRNA 33549805 33551762 33549805 ID=MS.gene000193.t1;Parent=MS.gene000193
chr2.1 exon 33551642 33551762 33551642 ID=MS.gene000193.t1.exon1;Parent=MS.gene000193.t1
chr2.1 CDS 33551642 33551762 33551642 ID=cds.MS.gene000193.t1;Parent=MS.gene000193.t1
chr2.1 exon 33550285 33551345 33550285 ID=MS.gene000193.t1.exon2;Parent=MS.gene000193.t1
chr2.1 CDS 33550285 33551345 33550285 ID=cds.MS.gene000193.t1;Parent=MS.gene000193.t1
chr2.1 exon 33550107 33550206 33550107 ID=MS.gene000193.t1.exon3;Parent=MS.gene000193.t1
chr2.1 CDS 33550107 33550206 33550107 ID=cds.MS.gene000193.t1;Parent=MS.gene000193.t1
chr2.1 exon 33549805 33550013 33549805 ID=MS.gene000193.t1.exon4;Parent=MS.gene000193.t1
chr2.1 CDS 33549805 33550013 33549805 ID=cds.MS.gene000193.t1;Parent=MS.gene000193.t1
Gene Sequence

>MS.gene000193

ATGGATAGTTGTCTTTCCTCATGTTGTTGTGTAGATAATCTCTTCAAGAGAAAGCAACTCCGTTCCCGTCCCATTGACACCATATTCAAGCTTCCAATTGCTCCAAATGCTTGGCCACCAGGTATTAATTTTGTGATTGTCAATTTCACACTTACGGGTTTAATATGATTTTTTCCTCTACATAAGGACTAAAATGGATTTTTTTTTCCAAGGAGACTAAAATGAATATTACACACAAATTAAAGGGATTAGTTGTATAATTGCAAGAATTATAAACAAAATATTTTTTATAGTAACTATTTAGTATGGTTACCAAAAATATACTTAAACTAGTGCTGGTGGGATTTCAGTTTTAGGTTATATATTCTAGTTAATGCATGATGATTATAACTAGTACTGTTGGATTGTTACATATAGGTAGTGGTTTTGCAAATGGAATTATTGATGTAGGTGGACTTCAAGTGTCACAAGTATCAACATTTAACAAAGTTTGGGCCACCTATGATGGTGGACCAGATAATCAAGGTGCCACAATATTTGAACCAACAGGTATACCTCAAGGATTCTCCATGTTAGGTAGCTACAGCCAACCAAACAATAAGCCTCTCTTTGGATATGTTCTTGTGGCAAAGGATGTTTCTTCAAGTACTAAAAATAGCACTTTAAAGAAACCAATTGATTATACACTTGTATTGAACACATCAACTATTAAGGTTAATCAAGATACCACATGTTATATTTGGTTACCAATAGCACCTGAAGGCTACAAATCATTAGGCCATGTTGTCACCACAACACAAGATAAACCTTCACTTGACAAAATCATGTGTGTCAGATCAGACCTCACAGACCAATGCGAGTCTTCTTCGTGGATTTGGGGAACAAACGACTTCAATTTTTATGATGTTAGACCAATCGATAGAGGTAGTCAAGCGGCTGGTGTTCACATCGGCACCTTTGTTGCACAAAATGGTGGGACAAATATTCCTCCATCTATTTCATGTTTGAAAAATCTCAATTCAATTTCAAAAATCATGCCTAATCTAGTACAAATTGATGCAATACTCAAAGTTTACTCTCCATTGATGTACTTGCATCCTGATGAAGAATACTTACCTTCCTCTGTCAATTGGTTTTTCTCCAATGGAGCATTACTATACAAGAAAGGTGAAGAATCAAATCCTGTTGCAATAGCACAAAATGGTACTAACCTTCCTCAGGATCCTAACAATGATGGTACCTATTGGCTTGACCTACCTTCTGATGATGCAAACAAAGAGAGGGTAAAAAAAGGTAATTTACAAAGTGCAGAATCTTATGTACATGTGAAACCAATGCTTGGAGGAACCTTCACAGATATTGCCATGTGGATTTTTTACCCTTTTAATGGACCTGGAAGAGCAAAAGTAAAGTTCATAAATGTTAAATTGGGGAAAATAGGTGAGCATGTTGGTGATTGGGAGCATGTGACCTTAAGGGTGAGTAATTTAGATGGTCAATTATGGCATGTATATTTCTCACAACATAGTAAAGGTGAATGGATTGATTCTTCTCAGCTTGAGTTTCAAACAAATAGACCAGTTTTTTATTCTTCATTGCATGGTCATGCATCATATCCACATGAAGGACTAGTTCTTCAAGGGAAAAATGGAATAGGTATTAGAAATGATAGTGCTAAGAGTGAAATGATTTTGGATTTGACAAAATTTGTATTGATTTCTGCTGATTATTTGGGGTCTTTTGTTATAGAGCCACCTTGGTTGCACTATTTTAGGAAATGGGGTCCAAAAATTGATTATAATATAGATGAGGAGTTGAGGAAGGTGGAGAAGATATTGCCATGGAAACTAAAGGATATTTTTGAAAATATTATTAGGAGTTTACCAGAAGAAGTGCTGGGAGAGGAAGGACCCACAGGTCCAAAGCTGAAGAATAATTGGAGTGGAGATGAAGTTTGA
Protein sequence

>MS.gene000193.t1

MDSCLSSCCCVDNLFKRKQLRSRPIDTIFKLPIAPNAWPPGSGFANGIIDVGGLQVSQVSTFNKVWATYDGGPDNQGATIFEPTGIPQGFSMLGSYSQPNNKPLFGYVLVAKDVSSSTKNSTLKKPIDYTLVLNTSTIKVNQDTTCYIWLPIAPEGYKSLGHVVTTTQDKPSLDKIMCVRSDLTDQCESSSWIWGTNDFNFYDVRPIDRGSQAAGVHIGTFVAQNGGTNIPPSISCLKNLNSISKIMPNLVQIDAILKVYSPLMYLHPDEEYLPSSVNWFFSNGALLYKKGEESNPVAIAQNGTNLPQDPNNDGTYWLDLPSDDANKERVKKGNLQSAESYVHVKPMLGGTFTDIAMWIFYPFNGPGRAKVKFINVKLGKIGEHVGDWEHVTLRLEFQTNRPVFYSSLHGHASYPHEGLVLQGKNGIEPPWLHYFRKWGPKIDYNIDEELRKVEKILPWKLKDIFENIIRSLPEEVLGEEGPTGPKLKNNWSGDEV