AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60889

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60889.t1 MTR_2g062430 95.660 553 24 0 1 553 1 553 0.0 1100
MS.gene60889.t1 MTR_2g062410 78.467 548 111 2 13 553 39 586 0.0 905
MS.gene60889.t1 MTR_2g062370 72.913 539 137 1 24 553 52 590 0.0 841
MS.gene60889.t1 MTR_4g131710 70.290 552 153 2 13 553 35 586 0.0 810
MS.gene60889.t1 MTR_2g062380 72.989 522 131 2 41 553 40 560 0.0 805
MS.gene60889.t1 MTR_2g067640 60.806 546 189 9 19 553 10 541 0.0 667
MS.gene60889.t1 MTR_2g062340 55.759 547 191 10 18 552 9 516 0.0 583
MS.gene60889.t1 MTR_1g080370 47.509 562 269 10 8 552 6 558 4.12e-176 509
MS.gene60889.t1 MTR_1g080410 47.509 562 269 10 8 552 6 558 4.12e-176 509
MS.gene60889.t1 MTR_5g007860 46.580 541 260 9 38 552 68 605 6.08e-170 495
MS.gene60889.t1 MTR_4g077620 46.235 571 284 13 1 552 1 567 5.10e-168 489
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60889.t1 AT2G44260 58.169 557 223 5 1 552 1 552 0.0 673
MS.gene60889.t1 AT2G44260 55.366 587 222 6 1 552 1 582 0.0 660
MS.gene60889.t1 AT2G44230 55.556 549 228 7 12 553 3 542 0.0 620
MS.gene60889.t1 AT3G01870 50.829 543 249 8 20 552 49 583 0.0 548
MS.gene60889.t1 AT3G01880 50.735 544 250 8 20 552 53 589 0.0 532
MS.gene60889.t1 AT5G18490 46.753 539 262 9 29 553 25 552 1.71e-170 494
MS.gene60889.t1 AT3G04350 46.422 545 268 9 29 553 26 566 4.39e-168 489
MS.gene60889.t1 AT3G04350 46.529 533 261 9 41 553 16 544 5.08e-165 480
MS.gene60889.t1 AT5G43950 44.649 542 281 9 29 552 24 564 2.28e-161 471
MS.gene60889.t1 AT1G04090 43.739 551 279 13 29 552 24 570 1.39e-158 464

Find 117 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTTCTGCTGATTATTT+AGG 0.124329 2.3:-31988585 MS.gene60889:CDS
GCTCTTCCTGGTCCATTAAA+AGG 0.142099 2.3:+31988918 None:intergenic
TGCTAAGAGTGAAATGATTT+TGG 0.186859 2.3:-31988625 MS.gene60889:CDS
TATCAACATTTAACAAAGTT+TGG 0.199497 2.3:-31989826 MS.gene60889:CDS
AAGTTCATAAATGTTAAATT+AGG 0.202582 2.3:-31988888 MS.gene60889:CDS
ACTTACCTTGCTCGGTTAAT+TGG 0.203183 2.3:-31989187 MS.gene60889:CDS
GCGAGTCTTCTTCGTGGATT+TGG 0.208690 2.3:-31989442 MS.gene60889:CDS
AAGATACCACATGTTATATT+TGG 0.212174 2.3:-31989577 MS.gene60889:CDS
CGAGTCTTCTTCGTGGATTT+GGG 0.218843 2.3:-31989441 MS.gene60889:CDS
GTTGAACACATCAACTATTA+AGG 0.248916 2.3:-31989606 MS.gene60889:CDS
TGATTTCTGCTGATTATTTA+GGG 0.258266 2.3:-31988584 MS.gene60889:CDS
GTCCAAAGCTGAAGAATAAT+TGG 0.267074 2.3:-31988380 MS.gene60889:CDS
TCCTCTCCCAGCACTTCTTC+TGG 0.294781 2.3:+31988414 None:intergenic
CTCCAATTATTCTTCAGCTT+TGG 0.329586 2.3:+31988378 None:intergenic
ATTGGGAGCATGTGACATTA+AGG 0.331662 2.3:-31988842 MS.gene60889:CDS
GGCTCATTCGTTGCACAAAA+TGG 0.331734 2.3:-31989347 MS.gene60889:CDS
GCAAATGGAATCATTGATCT+AGG 0.334564 2.3:-31989869 MS.gene60889:CDS
ATCAACATTTAACAAAGTTT+GGG 0.335558 2.3:-31989825 MS.gene60889:CDS
AATGTTAAATTAGGGAAAAT+AGG 0.338306 2.3:-31988879 MS.gene60889:CDS
GTAATTTAGATGGTCAATTA+TGG 0.338445 2.3:-31988815 MS.gene60889:CDS
GGCCAAGCATTTGGAGCAAT+TGG 0.358291 2.3:+31990443 None:intergenic
AGTTCATAAATGTTAAATTA+GGG 0.360247 2.3:-31988887 MS.gene60889:CDS
GCATCAATTTGTACTAGATT+AGG 0.374766 2.3:+31989260 None:intergenic
AGATGAGGAGTTGAGGAAAG+TGG 0.382577 2.3:-31988493 MS.gene60889:CDS
ATACCTGGTGGCCAAGCATT+TGG 0.390184 2.3:+31990434 None:intergenic
CCTAACATAGAGAATCCTTT+AGG 0.394355 2.3:+31989743 None:intergenic
ATTATCTGGTCCACCATCAT+AGG 0.395820 2.3:+31989799 None:intergenic
AGCAATTGGAAGCTTGAATA+TGG 0.401497 2.3:+31990457 None:intergenic
ATTGTGGCACCTTGATTATC+TGG 0.402581 2.3:+31989785 None:intergenic
AACACAAGTGAGTAATCAAT+TGG 0.404860 2.3:+31989626 None:intergenic
CCTAAAGCTTTGTATCCATT+AGG 0.407577 2.3:+31989542 None:intergenic
CCTAATGGATACAAAGCTTT+AGG 0.409137 2.3:-31989542 MS.gene60889:CDS
TTGTATCCATTAGGTGCTAT+TGG 0.410472 2.3:+31989551 None:intergenic
CCTAAAGGATTCTCTATGTT+AGG 0.416282 2.3:-31989743 MS.gene60889:CDS
CTAACAATGATGGTACCTAT+TGG 0.429733 2.3:-31989073 MS.gene60889:CDS
TTCTTCAGCTTTGGACCTGT+GGG 0.433050 2.3:+31988387 None:intergenic
CTTGTATAGTAATGCTCCAT+TGG 0.433338 2.3:+31989157 None:intergenic
CCTGTTCCAATAGCACAAAA+TGG 0.435052 2.3:-31989116 MS.gene60889:CDS
CAATAGGTACCATCATTGTT+AGG 0.437937 2.3:+31989074 None:intergenic
GCTTGAGTACCTCTATTGAT+TGG 0.438682 2.3:+31989386 None:intergenic
CAAGGTAAGTATTCTTCATC+AGG 0.438770 2.3:+31989200 None:intergenic
CTCTTCCTGGTCCATTAAAA+GGG 0.442838 2.3:+31988919 None:intergenic
TTCCAATTGCTCCAAATGCT+TGG 0.445747 2.3:-31990445 MS.gene60889:CDS
GAGTTTACCAGAAGAAGTGC+TGG 0.446564 2.3:-31988421 MS.gene60889:CDS
GCTCCAAATGCTTGGCCACC+AGG 0.451485 2.3:-31990437 MS.gene60889:intron
ATTCTTCAGCTTTGGACCTG+TGG 0.451562 2.3:+31988386 None:intergenic
CATGAAGGACTAGTTCTTCA+AGG 0.455081 2.3:-31988678 MS.gene60889:CDS
CACAACATAGTAAAGGTGAA+TGG 0.465087 2.3:-31988779 MS.gene60889:CDS
GTGAAGGTTCCTCCTAGCAT+TGG 0.467545 2.3:+31988966 None:intergenic
TGGCTACCAATAGCACCTAA+TGG 0.475290 2.3:-31989557 MS.gene60889:CDS
TAGGTGAACATGTTGGTGAT+TGG 0.478019 2.3:-31988860 MS.gene60889:CDS
AGAAGGTAGGTCAAGCCAAT+AGG 0.478817 2.3:+31989058 None:intergenic
AGCTTGAATATGGTGTCAAC+GGG 0.480366 2.3:+31990467 None:intergenic
AAAGTTTGGGCCACCTATGA+TGG 0.480692 2.3:-31989812 MS.gene60889:CDS
GCCACAATATTTGAACCAAC+AGG 0.497066 2.3:-31989770 MS.gene60889:CDS
ACAACATAGTAAAGGTGAAT+GGG 0.499232 2.3:-31988778 MS.gene60889:CDS
GAATATGGTGTCAACGGGAC+GGG 0.502650 2.3:+31990472 None:intergenic
TGGATATGCTCTTGTGGCAA+AGG 0.503902 2.3:-31989687 MS.gene60889:CDS
GAAGAAGTGCTGGGAGAGGA+AGG 0.504490 2.3:-31988411 MS.gene60889:CDS
TCAGGATGTAAGTACATCAA+TGG 0.505882 2.3:+31989218 None:intergenic
TTAAGGGTGAGTAATTTAGA+TGG 0.508208 2.3:-31988825 MS.gene60889:CDS
ACCAATGCGAGTCTTCTTCG+TGG 0.508222 2.3:-31989448 MS.gene60889:CDS
AGGTGAACATGTTGGTGATT+GGG 0.511142 2.3:-31988859 MS.gene60889:CDS
CTGGGAGAGGAAGGACCCAC+AGG 0.511477 2.3:-31988402 MS.gene60889:CDS
CAAGGTGGCTCTATAACAAA+AGG 0.513235 2.3:+31988561 None:intergenic
ACCTGTTGGTTCAAATATTG+TGG 0.520433 2.3:+31989769 None:intergenic
GAACCAACAGGTATACCTAA+AGG 0.523818 2.3:-31989758 MS.gene60889:CDS
CCTCAGGATCCTAACAATGA+TGG 0.535568 2.3:-31989083 MS.gene60889:CDS
AATAGAGGTACTCAAGCTGC+TGG 0.536143 2.3:-31989380 MS.gene60889:CDS
TGAAGAATACTTACCTTGCT+CGG 0.536982 2.3:-31989195 MS.gene60889:CDS
AAATGGTATTAACCTTCCTC+AGG 0.538181 2.3:-31989099 MS.gene60889:CDS
TGTTTGCATCATCAGAAGGT+AGG 0.539120 2.3:+31989045 None:intergenic
AAAGAGGTTTGTTGTTGGGT+TGG 0.542937 2.3:+31989711 None:intergenic
ACCAGAAGAAGTGCTGGGAG+AGG 0.544118 2.3:-31988415 MS.gene60889:CDS
TCATTCGTTGCACAAAATGG+TGG 0.544600 2.3:-31989344 MS.gene60889:CDS
TTCAAACATGAAATAGATGG+AGG 0.553594 2.3:+31989311 None:intergenic
CTTGTGTTGTGGTGACAACA+TGG 0.561956 2.3:+31989519 None:intergenic
TCTTTGTTTGCATCATCAGA+AGG 0.565098 2.3:+31989041 None:intergenic
TCCACGAAGAAGACTCGCAT+TGG 0.565993 2.3:+31989447 None:intergenic
GGAGCATTACTATACAAGAA+AGG 0.567167 2.3:-31989152 MS.gene60889:CDS
CATTGTTAGGATCCTGAGGA+AGG 0.572703 2.3:+31989087 None:intergenic
CCACATGGCAATATCTGTGA+AGG 0.573443 2.3:+31988950 None:intergenic
CAAGCTGCTGGTGTTCGTGT+CGG 0.577708 2.3:-31989368 MS.gene60889:CDS
ATAATATAGATGAGGAGTTG+AGG 0.582445 2.3:-31988500 MS.gene60889:CDS
AAGCTTGAATATGGTGTCAA+CGG 0.583843 2.3:+31990466 None:intergenic
CTGATGATGCAAACAAAGAG+AGG 0.585320 2.3:-31989037 MS.gene60889:CDS
GATGGTGGACCAGATAATCA+AGG 0.585776 2.3:-31989794 MS.gene60889:CDS
TATTTCTCACAACATAGTAA+AGG 0.585924 2.3:-31988786 MS.gene60889:CDS
AATCCTTTAGGTATACCTGT+TGG 0.587379 2.3:+31989755 None:intergenic
TGAATATGGTGTCAACGGGA+CGG 0.587459 2.3:+31990471 None:intergenic
GAGTCTTCTTCGTGGATTTG+GGG 0.588057 2.3:-31989440 MS.gene60889:CDS
ATGAAGGACTAGTTCTTCAA+GGG 0.592808 2.3:-31988677 MS.gene60889:CDS
TCAAACATGAAATAGATGGA+GGG 0.592826 2.3:+31989312 None:intergenic
CATTCGTTGCACAAAATGGT+GGG 0.596880 2.3:-31989343 MS.gene60889:CDS
GGGAAAATAGGTGAACATGT+TGG 0.598707 2.3:-31988867 MS.gene60889:CDS
GATGTTAGACCAATCAATAG+AGG 0.600235 2.3:-31989395 MS.gene60889:CDS
ATCTGGTCCACCATCATAGG+TGG 0.604053 2.3:+31989802 None:intergenic
TTCCTAAAATAGTGCAACCA+AGG 0.605969 2.3:+31988543 None:intergenic
CATGCATCATATCCACATGA+AGG 0.613328 2.3:-31988693 MS.gene60889:CDS
CCATCATTGTTAGGATCCTG+AGG 0.614872 2.3:+31989083 None:intergenic
GTACATGTGAAACCAATGCT+AGG 0.617083 2.3:-31988978 MS.gene60889:CDS
TTGGGAGCATGTGACATTAA+GGG 0.618313 2.3:-31988841 MS.gene60889:CDS
TGATGATGCAAACAAAGAGA+GGG 0.621716 2.3:-31989036 MS.gene60889:CDS
AGTTTACCAGAAGAAGTGCT+GGG 0.622675 2.3:-31988420 MS.gene60889:CDS
GGAAGGTTTATCTTGTGTTG+TGG 0.622916 2.3:+31989508 None:intergenic
AAGCTGAAGAATAATTGGAG+TGG 0.625543 2.3:-31988375 MS.gene60889:CDS
GTTTGGGCCACCTATGATGG+TGG 0.627591 2.3:-31989809 MS.gene60889:CDS
AAGTGGAGAAGATATTGCCA+TGG 0.635901 2.3:-31988476 MS.gene60889:CDS
AATGGAATCATTGATCTAGG+TGG 0.649257 2.3:-31989866 MS.gene60889:CDS
TGGTAGCCAAATATAACATG+TGG 0.651391 2.3:+31989571 None:intergenic
CTAAAATAGTGCAACCAAGG+TGG 0.652677 2.3:+31988546 None:intergenic
AATTGATTATAATATAGATG+AGG 0.654441 2.3:-31988508 MS.gene60889:CDS
TGAAGAACTAGTCCTTCATG+TGG 0.664200 2.3:+31988681 None:intergenic
AAGACTCGCATTGGTCTGTG+AGG 0.675872 2.3:+31989456 None:intergenic
CTTCATGTGGATATGATGCA+TGG 0.678841 2.3:+31988694 None:intergenic
CATGTGAAACCAATGCTAGG+AGG 0.684488 2.3:-31988975 MS.gene60889:CDS
CCTTCACAGATATTGCCATG+TGG 0.739729 2.3:-31988950 MS.gene60889:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAAAATTATTTATA+AGG + chr2.3:31988612-31988631 None:intergenic 0.0%
!!! TCTATATTATAATCAATTTT+TGG + chr2.3:31990388-31990407 None:intergenic 10.0%
!! AAGTTCATAAATGTTAAATT+AGG - chr2.3:31990010-31990029 MS.gene60889:intron 15.0%
!! AATTGATTATAATATAGATG+AGG - chr2.3:31990390-31990409 MS.gene60889:intron 15.0%
!! AGTTCATAAATGTTAAATTA+GGG - chr2.3:31990011-31990030 MS.gene60889:intron 15.0%
!!! ACATTTTTGTTTATAATTCT+TGG + chr2.3:31988862-31988881 None:intergenic 15.0%
!!! TTTTACTTAACTAAAAATCA+AGG - chr2.3:31988810-31988829 MS.gene60889:CDS 15.0%
!! AATGTTAAATTAGGGAAAAT+AGG - chr2.3:31990019-31990038 MS.gene60889:intron 20.0%
!! AGCAAAAAGTGATATATTTA+TGG - chr2.3:31988915-31988934 MS.gene60889:CDS 20.0%
!! ATCAACATTTAACAAAGTTT+GGG - chr2.3:31989073-31989092 MS.gene60889:CDS 20.0%
!! CTTATGTAGTCAATATTTAA+GGG + chr2.3:31988732-31988751 None:intergenic 20.0%
!! TATCAACATTTAACAAAGTT+TGG - chr2.3:31989072-31989091 MS.gene60889:CDS 20.0%
!!! AAAATATCTTTTAGTTTCCA+TGG + chr2.3:31990442-31990461 None:intergenic 20.0%
!!! TTTTTCAAACATGAAATAGA+TGG + chr2.3:31989593-31989612 None:intergenic 20.0%
! AAGATACCACATGTTATATT+TGG - chr2.3:31989321-31989340 MS.gene60889:CDS 25.0%
! CCTTATGTAGTCAATATTTA+AGG + chr2.3:31988733-31988752 None:intergenic 25.0%
! CTCAAACTAAAAGTAAAAAG+TGG + chr2.3:31988565-31988584 None:intergenic 25.0%
! GCATTAACTAGATATATAAC+AGG + chr2.3:31988961-31988980 None:intergenic 25.0%
! GTAAAAAGTGGCATATTTAA+AGG + chr2.3:31988553-31988572 None:intergenic 25.0%
! GTAATTTAGATGGTCAATTA+TGG - chr2.3:31990083-31990102 MS.gene60889:intron 25.0%
! TATTTCTCACAACATAGTAA+AGG - chr2.3:31990112-31990131 MS.gene60889:intron 25.0%
! TGCAATGAAGAATAAAAAAC+TGG + chr2.3:31990181-31990200 None:intergenic 25.0%
!! CCTTAAATATTGACTACATA+AGG - chr2.3:31988730-31988749 MS.gene60889:CDS 25.0%
!! TGATTTCTGCTGATTATTTA+GGG - chr2.3:31990314-31990333 MS.gene60889:intron 25.0%
!! TTAATACCATTTTGTGCTAT+TGG + chr2.3:31989791-31989810 None:intergenic 25.0%
!! TTGATTTCTGCTGATTATTT+AGG - chr2.3:31990313-31990332 MS.gene60889:intron 25.0%
!!! GTTTTTTATTCTTCATTGCA+TGG - chr2.3:31990181-31990200 MS.gene60889:intron 25.0%
!!! TGGATTTTTTACCCTTTTAA+TGG - chr2.3:31989968-31989987 MS.gene60889:intron 25.0%
AAAAGGGTAAAAAATCCACA+TGG + chr2.3:31989966-31989985 None:intergenic 30.0%
AACAAAGAGAGGGTAAAAAA+AGG - chr2.3:31989872-31989891 MS.gene60889:CDS 30.0%
AACACAAGTGAGTAATCAAT+TGG + chr2.3:31989275-31989294 None:intergenic 30.0%
ACAACATAGTAAAGGTGAAT+GGG - chr2.3:31990120-31990139 MS.gene60889:intron 30.0%
ATAATATAGATGAGGAGTTG+AGG - chr2.3:31990398-31990417 MS.gene60889:intron 30.0%
GCATCAATTTGTACTAGATT+AGG + chr2.3:31989641-31989660 None:intergenic 30.0%
GTTGAACACATCAACTATTA+AGG - chr2.3:31989292-31989311 MS.gene60889:CDS 30.0%
TCAAACATGAAATAGATGGA+GGG + chr2.3:31989589-31989608 None:intergenic 30.0%
TTAAGGGTGAGTAATTTAGA+TGG - chr2.3:31990073-31990092 MS.gene60889:intron 30.0%
TTCAAACATGAAATAGATGG+AGG + chr2.3:31989590-31989609 None:intergenic 30.0%
! AGTGTTGGATTGATACATAT+AGG - chr2.3:31988996-31989015 MS.gene60889:CDS 30.0%
! TGCTAAGAGTGAAATGATTT+TGG - chr2.3:31990273-31990292 MS.gene60889:intron 30.0%
!! GTTGCACTATTTTAGGAAAT+GGG - chr2.3:31990360-31990379 MS.gene60889:intron 30.0%
!! TTGCACTATTTTAGGAAATG+GGG - chr2.3:31990361-31990380 MS.gene60889:intron 30.0%
!!! GTTAATTGGTTTTTCTCCAA+TGG - chr2.3:31989725-31989744 MS.gene60889:CDS 30.0%
AAAAACCAATTAACCGAGCA+AGG + chr2.3:31989719-31989738 None:intergenic 35.0%
AAATGGTATTAACCTTCCTC+AGG - chr2.3:31989799-31989818 MS.gene60889:CDS 35.0%
AAGCTGAAGAATAATTGGAG+TGG - chr2.3:31990523-31990542 MS.gene60889:CDS 35.0%
AATCCTTTAGGTATACCTGT+TGG + chr2.3:31989146-31989165 None:intergenic 35.0%
AATGGAATCATTGATCTAGG+TGG - chr2.3:31989032-31989051 MS.gene60889:CDS 35.0%
ACAATCACACAATGAATACC+TGG + chr2.3:31988482-31988501 None:intergenic 35.0%
AGCAATTGGAAGCTTGAATA+TGG + chr2.3:31988444-31988463 None:intergenic 35.0%
CAAGGTAAGTATTCTTCATC+AGG + chr2.3:31989701-31989720 None:intergenic 35.0%
CAATAGGTACCATCATTGTT+AGG + chr2.3:31989827-31989846 None:intergenic 35.0%
CACAACATAGTAAAGGTGAA+TGG - chr2.3:31990119-31990138 MS.gene60889:intron 35.0%
CCTAAAGGATTCTCTATGTT+AGG - chr2.3:31989155-31989174 MS.gene60889:CDS 35.0%
CCTAACATAGAGAATCCTTT+AGG + chr2.3:31989158-31989177 None:intergenic 35.0%
CTAACAATGATGGTACCTAT+TGG - chr2.3:31989825-31989844 MS.gene60889:CDS 35.0%
CTCCAATTATTCTTCAGCTT+TGG + chr2.3:31990523-31990542 None:intergenic 35.0%
CTTCAAGGGAAAAATGGAAT+AGG - chr2.3:31990235-31990254 MS.gene60889:intron 35.0%
CTTGTATAGTAATGCTCCAT+TGG + chr2.3:31989744-31989763 None:intergenic 35.0%
GATGTTAGACCAATCAATAG+AGG - chr2.3:31989503-31989522 MS.gene60889:CDS 35.0%
GCAAATGGAATCATTGATCT+AGG - chr2.3:31989029-31989048 MS.gene60889:CDS 35.0%
GGATTGATACATATAGGTAG+TGG - chr2.3:31989002-31989021 MS.gene60889:CDS 35.0%
GTCCAAAGCTGAAGAATAAT+TGG - chr2.3:31990518-31990537 MS.gene60889:CDS 35.0%
TCAGGATGTAAGTACATCAA+TGG + chr2.3:31989683-31989702 None:intergenic 35.0%
TGAAGAATACTTACCTTGCT+CGG - chr2.3:31989703-31989722 MS.gene60889:CDS 35.0%
TGGTAGCCAAATATAACATG+TGG + chr2.3:31989330-31989349 None:intergenic 35.0%
TTCCTAAAATAGTGCAACCA+AGG + chr2.3:31990358-31990377 None:intergenic 35.0%
! AAGCTTGAATATGGTGTCAA+CGG + chr2.3:31988435-31988454 None:intergenic 35.0%
! ACAAGAGCATATCCAAAAAG+AGG + chr2.3:31989206-31989225 None:intergenic 35.0%
! ATGCTGATTATAGCTAGTGT+TGG - chr2.3:31988981-31989000 MS.gene60889:CDS 35.0%
! CCTAAAGCTTTGTATCCATT+AGG + chr2.3:31989359-31989378 None:intergenic 35.0%
! CCTAATGGATACAAAGCTTT+AGG - chr2.3:31989356-31989375 MS.gene60889:CDS 35.0%
! CTAGTTCTTCAAGGGAAAAA+TGG - chr2.3:31990229-31990248 MS.gene60889:intron 35.0%
! GACACACATGATTTTGTCAA+GGG + chr2.3:31989414-31989433 None:intergenic 35.0%
! GGAGCATTACTATACAAGAA+AGG - chr2.3:31989746-31989765 MS.gene60889:CDS 35.0%
! TCTTTGTTTGCATCATCAGA+AGG + chr2.3:31989860-31989879 None:intergenic 35.0%
! TCTTTTTGGATATGCTCTTG+TGG - chr2.3:31989205-31989224 MS.gene60889:CDS 35.0%
! TGACACACATGATTTTGTCA+AGG + chr2.3:31989415-31989434 None:intergenic 35.0%
! TGATGATGCAAACAAAGAGA+GGG - chr2.3:31989862-31989881 MS.gene60889:CDS 35.0%
! TTGTATCCATTAGGTGCTAT+TGG + chr2.3:31989350-31989369 None:intergenic 35.0%
!! AACTTTACTTTTGCTCTTCC+TGG + chr2.3:31989995-31990014 None:intergenic 35.0%
!! ACCTGTTGGTTCAAATATTG+TGG + chr2.3:31989132-31989151 None:intergenic 35.0%
!! ATAGGTAGTGGTTTTGCAAA+TGG - chr2.3:31989014-31989033 MS.gene60889:CDS 35.0%
!! ATGAAGGACTAGTTCTTCAA+GGG - chr2.3:31990221-31990240 MS.gene60889:intron 35.0%
!! CCAAAAAGAGGTTTGTTGTT+GGG + chr2.3:31989194-31989213 None:intergenic 35.0%
!! GGTTGCACTATTTTAGGAAA+TGG - chr2.3:31990359-31990378 MS.gene60889:intron 35.0%
!! TCCAAAAAGAGGTTTGTTGT+TGG + chr2.3:31989195-31989214 None:intergenic 35.0%
!! TTTTACCCTTTTAATGGACC+AGG - chr2.3:31989974-31989993 MS.gene60889:intron 35.0%
AAGTGGAGAAGATATTGCCA+TGG - chr2.3:31990422-31990441 MS.gene60889:intron 40.0%
ACTTACCTTGCTCGGTTAAT+TGG - chr2.3:31989711-31989730 MS.gene60889:CDS 40.0%
ATCACACAATGAATACCTGG+TGG + chr2.3:31988479-31988498 None:intergenic 40.0%
ATTATCTGGTCCACCATCAT+AGG + chr2.3:31989102-31989121 None:intergenic 40.0%
ATTGTGGCACCTTGATTATC+TGG + chr2.3:31989116-31989135 None:intergenic 40.0%
CATGCATCATATCCACATGA+AGG - chr2.3:31990205-31990224 MS.gene60889:intron 40.0%
CATTCGTTGCACAAAATGGT+GGG - chr2.3:31989555-31989574 MS.gene60889:CDS 40.0%
CCTGTTCCAATAGCACAAAA+TGG - chr2.3:31989782-31989801 MS.gene60889:CDS 40.0%
CTAAAATAGTGCAACCAAGG+TGG + chr2.3:31990355-31990374 None:intergenic 40.0%
CTCTTCCTGGTCCATTAAAA+GGG + chr2.3:31989982-31990001 None:intergenic 40.0%
CTTCATGTGGATATGATGCA+TGG + chr2.3:31990207-31990226 None:intergenic 40.0%
GAACCAACAGGTATACCTAA+AGG - chr2.3:31989140-31989159 MS.gene60889:CDS 40.0%
GCCACAATATTTGAACCAAC+AGG - chr2.3:31989128-31989147 MS.gene60889:CDS 40.0%
GCTTGAGTACCTCTATTGAT+TGG + chr2.3:31989515-31989534 None:intergenic 40.0%
GGAAGGTTTATCTTGTGTTG+TGG + chr2.3:31989393-31989412 None:intergenic 40.0%
GGGAAAATAGGTGAACATGT+TGG - chr2.3:31990031-31990050 MS.gene60889:intron 40.0%
GTACATGTGAAACCAATGCT+AGG - chr2.3:31989920-31989939 MS.gene60889:intron 40.0%
TCATTCGTTGCACAAAATGG+TGG - chr2.3:31989554-31989573 MS.gene60889:CDS 40.0%
TGAAGAACTAGTCCTTCATG+TGG + chr2.3:31990220-31990239 None:intergenic 40.0%
TGTTTGCATCATCAGAAGGT+AGG + chr2.3:31989856-31989875 None:intergenic 40.0%
TTCCAATTGCTCCAAATGCT+TGG - chr2.3:31988453-31988472 MS.gene60889:CDS 40.0%
! AGCTTGAATATGGTGTCAAC+GGG + chr2.3:31988434-31988453 None:intergenic 40.0%
! ATTGGGAGCATGTGACATTA+AGG - chr2.3:31990056-31990075 MS.gene60889:intron 40.0%
! CAAGGTGGCTCTATAACAAA+AGG + chr2.3:31990340-31990359 None:intergenic 40.0%
! CACATGATTTTGTCAAGGGA+AGG + chr2.3:31989410-31989429 None:intergenic 40.0%
! CACCTTGGTTGCACTATTTT+AGG - chr2.3:31990353-31990372 MS.gene60889:intron 40.0%
! CCCAACAACAAACCTCTTTT+TGG - chr2.3:31989191-31989210 MS.gene60889:CDS 40.0%
! CTGATGATGCAAACAAAGAG+AGG - chr2.3:31989861-31989880 MS.gene60889:CDS 40.0%
! TTGGGAGCATGTGACATTAA+GGG - chr2.3:31990057-31990076 MS.gene60889:intron 40.0%
!! AAAGAGGTTTGTTGTTGGGT+TGG + chr2.3:31989190-31989209 None:intergenic 40.0%
!! AGGTGAACATGTTGGTGATT+GGG - chr2.3:31990039-31990058 MS.gene60889:intron 40.0%
!! AGTTTACCAGAAGAAGTGCT+GGG - chr2.3:31990478-31990497 MS.gene60889:CDS 40.0%
!! CATGAAGGACTAGTTCTTCA+AGG - chr2.3:31990220-31990239 MS.gene60889:intron 40.0%
!! CCATTTTGTGCTATTGGAAC+AGG + chr2.3:31989785-31989804 None:intergenic 40.0%
!! CTTTTGTTATAGAGCCACCT+TGG - chr2.3:31990338-31990357 MS.gene60889:intron 40.0%
!! TAGGTGAACATGTTGGTGAT+TGG - chr2.3:31990038-31990057 MS.gene60889:intron 40.0%
AAAGTTTGGGCCACCTATGA+TGG - chr2.3:31989086-31989105 MS.gene60889:CDS 45.0%
AGAAGGTAGGTCAAGCCAAT+AGG + chr2.3:31989843-31989862 None:intergenic 45.0%
AGATGAGGAGTTGAGGAAAG+TGG - chr2.3:31990405-31990424 MS.gene60889:intron 45.0%
CATGTGAAACCAATGCTAGG+AGG - chr2.3:31989923-31989942 MS.gene60889:intron 45.0%
CATTGTTAGGATCCTGAGGA+AGG + chr2.3:31989814-31989833 None:intergenic 45.0%
CCACATGGCAATATCTGTGA+AGG + chr2.3:31989951-31989970 None:intergenic 45.0%
CCATCATTGTTAGGATCCTG+AGG + chr2.3:31989818-31989837 None:intergenic 45.0%
CCTCAGGATCCTAACAATGA+TGG - chr2.3:31989815-31989834 MS.gene60889:CDS 45.0%
CCTTCACAGATATTGCCATG+TGG - chr2.3:31989948-31989967 MS.gene60889:intron 45.0%
CGAGTCTTCTTCGTGGATTT+GGG - chr2.3:31989457-31989476 MS.gene60889:CDS 45.0%
CTTGTGTTGTGGTGACAACA+TGG + chr2.3:31989382-31989401 None:intergenic 45.0%
GAGTCTTCTTCGTGGATTTG+GGG - chr2.3:31989458-31989477 MS.gene60889:CDS 45.0%
GCTCTTCCTGGTCCATTAAA+AGG + chr2.3:31989983-31990002 None:intergenic 45.0%
GGCTCATTCGTTGCACAAAA+TGG - chr2.3:31989551-31989570 MS.gene60889:CDS 45.0%
TGGATATGCTCTTGTGGCAA+AGG - chr2.3:31989211-31989230 MS.gene60889:CDS 45.0%
TGGCTACCAATAGCACCTAA+TGG - chr2.3:31989341-31989360 MS.gene60889:CDS 45.0%
! GAGTTTACCAGAAGAAGTGC+TGG - chr2.3:31990477-31990496 MS.gene60889:CDS 45.0%
! TGAATATGGTGTCAACGGGA+CGG + chr2.3:31988430-31988449 None:intergenic 45.0%
!! AATAGAGGTACTCAAGCTGC+TGG - chr2.3:31989518-31989537 MS.gene60889:CDS 45.0%
!! ATTCTTCAGCTTTGGACCTG+TGG + chr2.3:31990515-31990534 None:intergenic 45.0%
!! GATGGTGGACCAGATAATCA+AGG - chr2.3:31989104-31989123 MS.gene60889:CDS 45.0%
!! TTCTTCAGCTTTGGACCTGT+GGG + chr2.3:31990514-31990533 None:intergenic 45.0%
!!! ATATTTTTGAAAATATTATT+AGG - chr2.3:31990455-31990474 MS.gene60889:CDS 5.0%
ACCAATGCGAGTCTTCTTCG+TGG - chr2.3:31989450-31989469 MS.gene60889:CDS 50.0%
ATACCTGGTGGCCAAGCATT+TGG + chr2.3:31988467-31988486 None:intergenic 50.0%
ATCTGGTCCACCATCATAGG+TGG + chr2.3:31989099-31989118 None:intergenic 50.0%
GCGAGTCTTCTTCGTGGATT+TGG - chr2.3:31989456-31989475 MS.gene60889:CDS 50.0%
GTGAAGGTTCCTCCTAGCAT+TGG + chr2.3:31989935-31989954 None:intergenic 50.0%
TCCACGAAGAAGACTCGCAT+TGG + chr2.3:31989454-31989473 None:intergenic 50.0%
! AAGACTCGCATTGGTCTGTG+AGG + chr2.3:31989445-31989464 None:intergenic 50.0%
! GAATATGGTGTCAACGGGAC+GGG + chr2.3:31988429-31988448 None:intergenic 50.0%
! GGCCAAGCATTTGGAGCAAT+TGG + chr2.3:31988458-31988477 None:intergenic 50.0%
CAAGCTGCTGGTGTTCGTGT+CGG - chr2.3:31989530-31989549 MS.gene60889:CDS 55.0%
GTTTGGGCCACCTATGATGG+TGG - chr2.3:31989089-31989108 MS.gene60889:CDS 55.0%
TCCTCTCCCAGCACTTCTTC+TGG + chr2.3:31990487-31990506 None:intergenic 55.0%
!! ACCAGAAGAAGTGCTGGGAG+AGG - chr2.3:31990483-31990502 MS.gene60889:CDS 55.0%
!! GAAGAAGTGCTGGGAGAGGA+AGG - chr2.3:31990487-31990506 MS.gene60889:CDS 55.0%
GCTCCAAATGCTTGGCCACC+AGG - chr2.3:31988461-31988480 MS.gene60889:CDS 60.0%
CTGGGAGAGGAAGGACCCAC+AGG - chr2.3:31990496-31990515 MS.gene60889:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 31988362 31990558 31988362 ID=MS.gene60889
chr2.3 mRNA 31988362 31990558 31988362 ID=MS.gene60889.t1;Parent=MS.gene60889
chr2.3 exon 31990438 31990558 31990438 ID=MS.gene60889.t1.exon1;Parent=MS.gene60889.t1
chr2.3 CDS 31990438 31990558 31990438 ID=cds.MS.gene60889.t1;Parent=MS.gene60889.t1
chr2.3 exon 31988362 31989902 31988362 ID=MS.gene60889.t1.exon2;Parent=MS.gene60889.t1
chr2.3 CDS 31988362 31989902 31988362 ID=cds.MS.gene60889.t1;Parent=MS.gene60889.t1
Gene Sequence

>MS.gene60889

ATGGATAATTGTCTTTCTTCATGTTGTTGTGTAGATAATCTCTTCAAGAGAAAGCAATCTCGATCCCGTCCCGTTGACACCATATTCAAGCTTCCAATTGCTCCAAATGCTTGGCCACCAGGTATTCATTGTGTGATTGTCAATTTCAGTCTTCTTGTGATATTATATAAGTTTTATATGATTTTTTTCCTTTAAATATGCCACTTTTTACTTTTAGTTTGAGTAAAAAATATTTCAAGATTTTATTCCTTATAAATAATTTTTATTTTATTTTTTTGCTTTTGATCTTTGTAGTTTCTTTTTAGTTGCTGAAATATTTTTTACGTTAGAATTGATCATTTTAATATGTAAAATCTTTGATTTTAGTCCCTTAAATATTGACTACATAAGGACTAATTAAAATGAATGTTACACTAATATTACATTGATTAATTGAAGCATGAAGAAATTTTACTTAACTAAAAATCAAGGATCAAAAGTAAAAAAGTTGTATGTTTCCAAGAATTATAAACAAAAATGTTTTTACATAAACTATGTTTTTACATAAACTAAAAGCAAAAAGTGATATATTTATGGTTATCAAAAATATATTTAAACCTGTTATATATCTAGTTAATGCATGCTGATTATAGCTAGTGTTGGATTGATACATATAGGTAGTGGTTTTGCAAATGGAATCATTGATCTAGGTGGACTTCAAGTGTCACAAGTATCAACATTTAACAAAGTTTGGGCCACCTATGATGGTGGACCAGATAATCAAGGTGCCACAATATTTGAACCAACAGGTATACCTAAAGGATTCTCTATGTTAGGTAGCTACAGCCAACCCAACAACAAACCTCTTTTTGGATATGCTCTTGTGGCAAAGGATGTTTCTTCAAGTACTACAAATAGCACTTTAAAGAAACCAATTGATTACTCACTTGTGTTGAACACATCAACTATTAAGGTTAATCAAGATACCACATGTTATATTTGGCTACCAATAGCACCTAATGGATACAAAGCTTTAGGCCATGTTGTCACCACAACACAAGATAAACCTTCCCTTGACAAAATCATGTGTGTCAGATCAGACCTCACAGACCAATGCGAGTCTTCTTCGTGGATTTGGGGATCAAACGACTTCAATTTTTATGATGTTAGACCAATCAATAGAGGTACTCAAGCTGCTGGTGTTCGTGTCGGCTCATTCGTTGCACAAAATGGTGGGACTAATGTCCCTCCATCTATTTCATGTTTGAAAAATCTCAATTCAATTTCAAAAATCATGCCTAATCTAGTACAAATTGATGCAATACTCAAAGTTTACTCTCCATTGATGTACTTACATCCTGATGAAGAATACTTACCTTGCTCGGTTAATTGGTTTTTCTCCAATGGAGCATTACTATACAAGAAAGGTGAAGAGTCAAATCCTGTTCCAATAGCACAAAATGGTATTAACCTTCCTCAGGATCCTAACAATGATGGTACCTATTGGCTTGACCTACCTTCTGATGATGCAAACAAAGAGAGGGTAAAAAAAGGAAATTTACAAAGTGCAGAATCTTATGTACATGTGAAACCAATGCTAGGAGGAACCTTCACAGATATTGCCATGTGGATTTTTTACCCTTTTAATGGACCAGGAAGAGCAAAAGTAAAGTTCATAAATGTTAAATTAGGGAAAATAGGTGAACATGTTGGTGATTGGGAGCATGTGACATTAAGGGTGAGTAATTTAGATGGTCAATTATGGCATGTATATTTCTCACAACATAGTAAAGGTGAATGGGTTGATTCTTCTCAGCTTGAGTTTCAAACAAATAGACCAGTTTTTTATTCTTCATTGCATGGCCATGCATCATATCCACATGAAGGACTAGTTCTTCAAGGGAAAAATGGAATAGGTGTTAGAAATGATAGTGCTAAGAGTGAAATGATTTTGGATTTGACAAAATTTGTATTGATTTCTGCTGATTATTTAGGGCCTTTTGTTATAGAGCCACCTTGGTTGCACTATTTTAGGAAATGGGGTCCAAAAATTGATTATAATATAGATGAGGAGTTGAGGAAAGTGGAGAAGATATTGCCATGGAAACTAAAAGATATTTTTGAAAATATTATTAGGAGTTTACCAGAAGAAGTGCTGGGAGAGGAAGGACCCACAGGTCCAAAGCTGAAGAATAATTGGAGTGGAGATGAAGTTTGA
Protein sequence

>MS.gene60889.t1

MDNCLSSCCCVDNLFKRKQSRSRPVDTIFKLPIAPNAWPPGSGFANGIIDLGGLQVSQVSTFNKVWATYDGGPDNQGATIFEPTGIPKGFSMLGSYSQPNNKPLFGYALVAKDVSSSTTNSTLKKPIDYSLVLNTSTIKVNQDTTCYIWLPIAPNGYKALGHVVTTTQDKPSLDKIMCVRSDLTDQCESSSWIWGSNDFNFYDVRPINRGTQAAGVRVGSFVAQNGGTNVPPSISCLKNLNSISKIMPNLVQIDAILKVYSPLMYLHPDEEYLPCSVNWFFSNGALLYKKGEESNPVPIAQNGINLPQDPNNDGTYWLDLPSDDANKERVKKGNLQSAESYVHVKPMLGGTFTDIAMWIFYPFNGPGRAKVKFINVKLGKIGEHVGDWEHVTLRVSNLDGQLWHVYFSQHSKGEWVDSSQLEFQTNRPVFYSSLHGHASYPHEGLVLQGKNGIGVRNDSAKSEMILDLTKFVLISADYLGPFVIEPPWLHYFRKWGPKIDYNIDEELRKVEKILPWKLKDIFENIIRSLPEEVLGEEGPTGPKLKNNWSGDEV