AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60884

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60884.t1 MTR_2g062380 94.242 521 30 0 241 761 40 560 0.0 1021
MS.gene60884.t1 MTR_2g062380 54.054 185 80 3 24 208 32 211 9.81e-53 192
MS.gene60884.t1 MTR_2g062370 81.413 538 99 1 225 761 53 590 0.0 920
MS.gene60884.t1 MTR_2g062370 53.608 194 84 4 16 208 53 241 5.19e-52 191
MS.gene60884.t1 MTR_2g062410 79.279 555 108 3 210 761 36 586 0.0 917
MS.gene60884.t1 MTR_2g062410 55.000 200 84 4 10 208 47 241 2.25e-59 211
MS.gene60884.t1 MTR_2g062430 70.364 550 153 2 213 761 13 553 0.0 815
MS.gene60884.t1 MTR_2g062430 59.135 208 79 4 2 208 11 213 1.86e-68 236
MS.gene60884.t1 MTR_4g131710 68.649 555 171 2 210 761 32 586 0.0 795
MS.gene60884.t1 MTR_4g131710 56.410 195 79 4 16 209 47 236 4.98e-61 216
MS.gene60884.t1 MTR_2g067640 53.748 547 222 8 225 761 16 541 0.0 598
MS.gene60884.t1 MTR_2g067640 60.976 205 65 5 11 208 10 206 1.56e-68 235
MS.gene60884.t1 MTR_2g062340 50.000 554 220 10 218 760 9 516 5.65e-176 514
MS.gene60884.t1 MTR_2g062340 91.866 209 17 0 1 209 1 209 4.97e-132 402
MS.gene60884.t1 MTR_1g080370 46.069 547 265 9 231 760 25 558 2.12e-160 476
MS.gene60884.t1 MTR_1g080370 37.879 198 110 3 20 209 23 215 1.36e-34 139
MS.gene60884.t1 MTR_1g080410 46.069 547 265 9 231 760 25 558 2.12e-160 476
MS.gene60884.t1 MTR_1g080410 37.879 198 110 3 20 209 23 215 1.36e-34 139
MS.gene60884.t1 MTR_5g007860 43.455 573 288 10 215 760 42 605 2.26e-155 465
MS.gene60884.t1 MTR_5g007860 38.571 210 113 5 15 209 54 262 3.12e-34 139
MS.gene60884.t1 MTR_4g077620 43.557 551 277 12 231 760 30 567 1.42e-144 436
MS.gene60884.t1 MTR_4g077620 41.232 211 110 7 10 209 19 226 2.18e-36 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60884.t1 AT2G44260 52.095 549 251 5 214 760 14 552 0.0 591
MS.gene60884.t1 AT2G44260 51.500 200 90 2 9 208 18 210 8.37e-58 206
MS.gene60884.t1 AT2G44260 53.640 522 230 5 241 760 71 582 0.0 582
MS.gene60884.t1 AT2G44260 53.005 183 79 2 26 208 65 240 5.33e-53 194
MS.gene60884.t1 AT2G44230 50.814 553 254 7 213 761 4 542 0.0 558
MS.gene60884.t1 AT2G44230 52.308 195 83 5 16 208 16 202 1.13e-52 192
MS.gene60884.t1 AT3G01870 46.000 550 273 8 220 760 49 583 4.12e-168 497
MS.gene60884.t1 AT3G01870 42.500 200 100 6 16 208 54 245 9.59e-38 149
MS.gene60884.t1 AT3G01880 46.055 545 272 8 225 760 58 589 5.87e-165 489
MS.gene60884.t1 AT3G01880 46.000 200 93 6 16 208 58 249 1.50e-40 158
MS.gene60884.t1 AT5G18490 44.669 544 270 9 231 761 27 552 6.95e-157 467
MS.gene60884.t1 AT5G18490 36.923 195 106 4 20 209 25 207 4.22e-32 132
MS.gene60884.t1 AT3G04350 43.739 551 278 9 231 761 28 566 2.25e-152 456
MS.gene60884.t1 AT3G04350 35.714 196 113 4 20 206 26 217 2.50e-28 120
MS.gene60884.t1 AT3G04350 43.438 541 274 9 241 761 16 544 2.49e-147 442
MS.gene60884.t1 AT3G04350 34.595 185 108 4 31 206 15 195 3.76e-24 107
MS.gene60884.t1 AT5G43950 43.169 549 289 9 228 760 23 564 5.97e-146 439
MS.gene60884.t1 AT5G43950 37.500 208 124 4 7 209 10 216 1.82e-34 139
MS.gene60884.t1 AT1G04090 42.397 559 285 13 228 760 23 570 2.57e-144 436
MS.gene60884.t1 AT1G04090 35.149 202 118 7 19 209 23 222 1.26e-25 112
MS.gene60884.t1 AT4G17140 25.434 346 182 17 81 381 1995 2309 3.72e-11 67.8
MS.gene60884.t1 AT4G17140 25.434 346 182 17 81 381 1985 2299 3.72e-11 67.8
MS.gene60884.t1 AT4G17140 25.434 346 182 17 81 381 2021 2335 3.78e-11 67.8
MS.gene60884.t1 AT1G48090 24.485 388 235 20 36 385 2025 2392 3.98e-11 67.8
MS.gene60884.t1 AT1G48090 25.000 388 233 21 36 385 2044 2411 4.33e-11 67.4
MS.gene60884.t1 AT1G48090 25.000 388 233 21 36 385 2003 2370 4.36e-11 67.4
MS.gene60884.t1 AT1G48090 25.000 388 233 21 36 385 2003 2370 4.36e-11 67.4

Find 170 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACTCTCCTTTGTATATT+TGG 0.127143 2.3:+32025767 MS.gene60884:CDS
ATCACATACAAGGCTATATT+TGG 0.213661 2.3:+32024260 MS.gene60884:CDS
ACTTACCTTCCTCGGTTAAT+TGG 0.215430 2.3:+32026157 MS.gene60884:CDS
GCTTTAATTCCTCTATTTAT+TGG 0.227005 2.3:-32025958 None:intergenic
TTGGTTTCTGCAGAATATTT+AGG 0.246737 2.3:+32026786 MS.gene60884:CDS
TTCCTGTTTCACAACCTTCT+TGG 0.254181 2.3:+32025344 MS.gene60884:CDS
CTTGTAGCAAAGGATATTTC+AGG 0.255851 2.3:+32025670 MS.gene60884:CDS
AGAGTTTATAGATATTAAAT+TGG 0.261098 2.3:+32026455 MS.gene60884:CDS
TCCTCTCCCAAGGCTTCTTT+TGG 0.261151 2.3:-32026957 None:intergenic
TTTCAACATTCAACAAAGTT+TGG 0.264610 2.3:+32024014 MS.gene60884:CDS
CTCAACACAATGCTGGTAAA+TGG 0.273775 2.3:+32026565 MS.gene60884:CDS
AAAACCACTTGATTACAAAT+TGG 0.273804 2.3:+32024207 MS.gene60884:CDS
CATCAACATTCAGCAAAGTT+TGG 0.275579 2.3:+32025521 MS.gene60884:CDS
AGGTAGAAATGTTATGGATA+TGG 0.277907 2.3:+32026752 MS.gene60884:CDS
GTGAATCGTCTACATTGATT+TGG 0.279504 2.3:+32025902 MS.gene60884:CDS
GTCCAAAGGAAAAGAGTAAT+TGG 0.284986 2.3:+32026991 MS.gene60884:CDS
ATTGGGAGCATGTGACATTA+AGG 0.285039 2.3:+32026502 MS.gene60884:CDS
TGGTTTCTGCAGAATATTTA+GGG 0.297210 2.3:+32026787 MS.gene60884:CDS
GGCACCTTTGTTGCTCAAAT+TGG 0.299661 2.3:+32025997 MS.gene60884:CDS
TGAATCGTCTACATTGATTT+GGG 0.313780 2.3:+32025903 MS.gene60884:CDS
AACTCTACTTTAGCCCTTCC+AGG 0.316265 2.3:-32026438 None:intergenic
ATCAACATTCAGCAAAGTTT+GGG 0.318101 2.3:+32025522 MS.gene60884:CDS
GAGTTTATAGATATTAAATT+GGG 0.325734 2.3:+32026456 MS.gene60884:CDS
CTTCATGGTTGGGTTCTTGT+AGG 0.329796 2.3:+32024148 MS.gene60884:CDS
GATGACTGTGTTTGCAAAAC+TGG 0.332977 2.3:-32025470 None:intergenic
GTAACTTCGATGGTCAATTA+TGG 0.344009 2.3:+32026529 MS.gene60884:CDS
GGCCAAGAAGGTTGTGAAAC+AGG 0.353292 2.3:-32025346 None:intergenic
CATGCTGGCATTAATCTACT+AGG 0.355903 2.3:+32026693 MS.gene60884:CDS
GGAAGGTTTATCTTGTGTTT+TGG 0.358980 2.3:-32025836 None:intergenic
ATCATCTGGTCCACCTTCAT+TGG 0.362805 2.3:-32024041 None:intergenic
GAACCAACAGGACTGACTTA+TGG 0.368553 2.3:+32025589 MS.gene60884:CDS
GCTTCAACACCTCTCTTGAT+TGG 0.379642 2.3:-32024463 None:intergenic
CCAAACAACAAACCTCTTCA+TGG 0.383735 2.3:+32024133 MS.gene60884:CDS
TAATAAAGGTAGAAATGTTA+TGG 0.389081 2.3:+32026746 MS.gene60884:CDS
CCATGAAGAGGTTTGTTGTT+TGG 0.390861 2.3:-32024133 None:intergenic
CTAACACTGATGGTTCCTAT+TGG 0.400945 2.3:+32026271 MS.gene60884:CDS
ATTGTTTATGCTTCTTTGCA+TGG 0.404938 2.3:+32026654 MS.gene60884:CDS
GGGCGCCTACGACGGTGGAC+CGG 0.407167 2.3:+32025543 MS.gene60884:CDS
GCAAACACAGTCATCAATCT+AGG 0.408168 2.3:+32025478 MS.gene60884:CDS
CAATAGGAACCATCAGTGTT+AGG 0.411818 2.3:-32026270 None:intergenic
AATACAAGTCAATGAAGCTT+TGG 0.413133 2.3:+32024401 MS.gene60884:CDS
TTCAAACATGAAATTGATGA+AGG 0.414574 2.3:-32026033 None:intergenic
AATGTGGCTAAGGCGTTGCC+TGG 0.415179 2.3:+32026894 MS.gene60884:CDS
GCAAGAGATGATACTAATAA+AGG 0.425735 2.3:+32026732 MS.gene60884:CDS
TCTGACACACATGACTTTGT+TGG 0.429774 2.3:-32025860 None:intergenic
CCTGTTCCCATAGCACAAAA+TGG 0.434660 2.3:+32026228 MS.gene60884:CDS
CAACAAACCTCTTCATGGTT+GGG 0.434795 2.3:+32024138 MS.gene60884:CDS
ATTGTAACTCCAAGATCATC+TGG 0.435507 2.3:-32024055 None:intergenic
GGTAGAAATGTTATGGATAT+GGG 0.435541 2.3:+32026753 MS.gene60884:CDS
GGATATGGGAAAATATGTAT+TGG 0.435630 2.3:+32026767 MS.gene60884:CDS
GCAAGTGGCATCATTGATCT+TGG 0.444135 2.3:+32023968 MS.gene60884:CDS
TGTCTAGTGAAGGCTTCTCT+TGG 0.444407 2.3:-32024336 None:intergenic
CTTCATCAGAATGTAAGTAT+AGG 0.445447 2.3:-32026131 None:intergenic
GTCTAGTGAAGGCTTCTCTT+GGG 0.445960 2.3:-32024335 None:intergenic
GATATTAAATTGGGGAAAGC+AGG 0.451140 2.3:+32026465 MS.gene60884:CDS
ACTTATGGATACTCTATGTT+AGG 0.451687 2.3:+32025604 MS.gene60884:CDS
AAAGGTGCCAACTCGAACAC+CGG 0.452137 2.3:-32025983 None:intergenic
GGCGCCTACGACGGTGGACC+GGG 0.458088 2.3:+32025544 MS.gene60884:CDS
CCTGATGGCTACAAAGTTGT+TGG 0.462939 2.3:+32024295 MS.gene60884:CDS
TGTTGGTCCTTCCTCTCCCA+AGG 0.465771 2.3:-32026967 None:intergenic
TCTTTGTTTGCATTATCAGC+AGG 0.468164 2.3:-32026303 None:intergenic
CTTGTATAGTAATGCTCCGT+TGG 0.470906 2.3:-32026187 None:intergenic
CAGGTGAACATGTTGGTGAT+TGG 0.472172 2.3:+32026484 MS.gene60884:CDS
ACAACAAACCTCTTCATGGT+TGG 0.474915 2.3:+32024137 MS.gene60884:CDS
ATAGGAATGGAGCGTTAACT+TGG 0.477931 2.3:-32026113 None:intergenic
TGCAAAGAAGCATAAACAAT+AGG 0.479629 2.3:-32026651 None:intergenic
GTTTGGGGCGCCTACGACGG+TGG 0.480695 2.3:+32025538 MS.gene60884:CDS
GAAGGTGGACCAGATGATCT+TGG 0.482031 2.3:+32024046 MS.gene60884:CDS
TTGGGAGAGGAAGGACCAAC+AGG 0.483726 2.3:+32026969 MS.gene60884:CDS
ACCTTTGTTGCTCAAATTGG+TGG 0.486604 2.3:+32026000 MS.gene60884:CDS
TTGTAGCCATCAGGTGCTAC+TGG 0.488029 2.3:-32024286 None:intergenic
TGGTAGCCATGAGGTGCTAT+TGG 0.489916 2.3:-32025793 None:intergenic
GAGTTTACCAAAAGAAGCCT+TGG 0.494113 2.3:+32026950 MS.gene60884:CDS
ACTGAAGCGCCTCGATTGCC+CGG 0.495851 2.3:-32025562 None:intergenic
TCCTTTCAATGGACCTGGAA+GGG 0.497466 2.3:+32026425 MS.gene60884:CDS
CCAACAACTTTGTAGCCATC+AGG 0.498774 2.3:-32024295 None:intergenic
ACATTGATTTGGGGATCAAA+CGG 0.501887 2.3:+32025913 MS.gene60884:CDS
GGCATCATTGATCTTGGTGG+TGG 0.502840 2.3:+32023974 MS.gene60884:CDS
GCCCTTCCAGGTCCATTGAA+AGG 0.503272 2.3:-32026426 None:intergenic
CTAAAATAATTCAACCATGC+TGG 0.503659 2.3:-32026825 None:intergenic
GCTTCAGTTTATGAACCAAC+AGG 0.505107 2.3:+32025577 MS.gene60884:CDS
TGGTTATGTTCTTGTAGCAA+AGG 0.506215 2.3:+32025660 MS.gene60884:CDS
ATGATACCTTCTGGCCAAGA+AGG 0.506844 2.3:-32025358 None:intergenic
AGGTGAACATGTTGGTGATT+GGG 0.508342 2.3:+32026485 MS.gene60884:CDS
CCTTTGTTGCTCAAATTGGT+GGG 0.510589 2.3:+32026001 MS.gene60884:CDS
AGCAGGTAGGTCAAGCCAAT+AGG 0.513437 2.3:-32026286 None:intergenic
CACATGACTTTGTTGGTGGA+AGG 0.513673 2.3:-32025853 None:intergenic
AGTTTACCAAAAGAAGCCTT+GGG 0.519013 2.3:+32026951 MS.gene60884:CDS
TTGGGAGCATGTGACATTAA+GGG 0.526297 2.3:+32026503 MS.gene60884:CDS
TCTAGATCAAAGTCAATATG+TGG 0.527060 2.3:-32026858 None:intergenic
TTAAATGAGGTTTGAATGAG+TGG 0.529573 2.3:-32023592 None:intergenic
GAAGAGGTTTGTTGTTTGGT+TGG 0.530138 2.3:-32024129 None:intergenic
AAACCATCTCTTAACTTCCC+AGG 0.534968 2.3:-32026912 None:intergenic
GTGAATGTTCCTCCATACAT+TGG 0.538131 2.3:-32026378 None:intergenic
CATACCAATTTGTAATCAAG+TGG 0.538139 2.3:-32024211 None:intergenic
AAGAGAGGTGTTGAAGCTAA+AGG 0.538834 2.3:+32024469 MS.gene60884:CDS
AAAGAGGCTTGCTGTTGGGT+TGG 0.538892 2.3:-32025636 None:intergenic
TGAAGAATACTTACCTTCCT+CGG 0.539422 2.3:+32026149 MS.gene60884:CDS
TCACAACCTTCTTGGCCAGA+AGG 0.539430 2.3:+32025352 MS.gene60884:CDS
TCAGAATGTAAGTATAGGAA+TGG 0.541007 2.3:-32026126 None:intergenic
GAACCAACAGGTTTATCTGA+AGG 0.541211 2.3:+32024082 MS.gene60884:CDS
CCATCAGTGTTAGGATCTTG+AGG 0.541983 2.3:-32026261 None:intergenic
TACTTCTCTCAACACAATGC+TGG 0.544284 2.3:+32026558 MS.gene60884:CDS
GTACATGTGAAACCAATGTA+TGG 0.546199 2.3:+32026366 MS.gene60884:CDS
GGAAGGACCAACAGGTCCAA+AGG 0.546506 2.3:+32026977 MS.gene60884:CDS
ACCAAAAGAAGCCTTGGGAG+AGG 0.547085 2.3:+32026956 MS.gene60884:CDS
CAAAACAAACATCACATACA+AGG 0.548841 2.3:+32024250 MS.gene60884:CDS
GACGGTGGACCGGGCAATCG+AGG 0.551257 2.3:+32025553 MS.gene60884:CDS
AAAGAAGCCTTGGGAGAGGA+AGG 0.551794 2.3:+32026960 MS.gene60884:CDS
CCCACCAATTTGAGCAACAA+AGG 0.552223 2.3:-32026001 None:intergenic
AAAGTTTGGACAACCAATGA+AGG 0.552963 2.3:+32024028 MS.gene60884:CDS
ATCCTTTCAATGGACCTGGA+AGG 0.553889 2.3:+32026424 MS.gene60884:CDS
TCAAACATGAAATTGATGAA+GGG 0.554987 2.3:-32026032 None:intergenic
ATTGCCCGGTCCACCGTCGT+AGG 0.557656 2.3:-32025548 None:intergenic
GACACACATGACTTTGTTGG+TGG 0.559758 2.3:-32025857 None:intergenic
CCTCAAGATCCTAACACTGA+TGG 0.563292 2.3:+32026261 MS.gene60884:CDS
AATAGAGGAATTAAAGCTCC+CGG 0.563330 2.3:+32025964 MS.gene60884:CDS
ATTCACTGATATTGCCATGT+GGG 0.565127 2.3:+32026395 MS.gene60884:CDS
TGAATTGCAGAATGTGGCTA+AGG 0.571192 2.3:+32026884 MS.gene60884:CDS
AACAAAGAGAGAGTCAAGAA+AGG 0.571578 2.3:+32026318 MS.gene60884:CDS
TATCCATAAGTCAGTCCTGT+TGG 0.574072 2.3:-32025592 None:intergenic
AAACCTTCAGATAAACCTGT+TGG 0.574604 2.3:-32024085 None:intergenic
ATGCTGGCATTAATCTACTA+GGG 0.576721 2.3:+32026694 MS.gene60884:CDS
ATCAGCAGGAAGCTTAAATG+AGG 0.577159 2.3:-32023605 None:intergenic
CATGCATCATATCCACATGC+TGG 0.577243 2.3:+32026678 MS.gene60884:CDS
GATATTTCAGGAAAGAGTAA+TGG 0.579239 2.3:+32025682 MS.gene60884:CDS
GGAGCATTACTATACAAGAA+AGG 0.581086 2.3:+32026192 MS.gene60884:CDS
AAAGCTCCCGGTGTTCGAGT+TGG 0.582792 2.3:+32025976 MS.gene60884:CDS
CAGTGTTAGGATCTTGAGGA+AGG 0.583165 2.3:-32026257 None:intergenic
GTAGTGAATGTGGCGAGAGC+GGG 0.585956 2.3:-32025311 None:intergenic
ATGTGGCTAAGGCGTTGCCT+GGG 0.589419 2.3:+32026895 MS.gene60884:CDS
GATGTTAGACCAATAAATAG+AGG 0.590723 2.3:+32025949 MS.gene60884:CDS
TTGCCTGGGAAGTTAAGAGA+TGG 0.591065 2.3:+32026909 MS.gene60884:CDS
CTGAAGTTAAAGAGCCAGCA+TGG 0.591737 2.3:+32026811 MS.gene60884:CDS
TTTGGAGAACAGAAAACAAG+AGG 0.597580 2.3:+32024419 MS.gene60884:CDS
TGTTTGCATTATCAGCAGGT+AGG 0.599393 2.3:-32026299 None:intergenic
AGTTTATAGATATTAAATTG+GGG 0.600504 2.3:+32026457 MS.gene60884:CDS
AAAGTTTGGGGCGCCTACGA+CGG 0.602534 2.3:+32025535 MS.gene60884:CDS
CTTGGGAAGTAGTGACAACA+TGG 0.602691 2.3:-32024318 None:intergenic
CCTCATGGCTACCATGCTGT+AGG 0.608416 2.3:+32025802 MS.gene60884:CDS
TGGTTACCAGTAGCACCTGA+TGG 0.610702 2.3:+32024280 MS.gene60884:CDS
AAGGAAAAGAGTAATTGGAA+TGG 0.612931 2.3:+32026996 MS.gene60884:CDS
ACAAGAACCCAACCATGAAG+AGG 0.613128 2.3:-32024145 None:intergenic
CAAAACTACTAGCATCACTT+CGG 0.616275 2.3:-32026611 None:intergenic
ATTGAACACTGCATCCGCCA+CGG 0.616955 2.3:+32025738 MS.gene60884:CDS
GAATCGTCTACATTGATTTG+GGG 0.618231 2.3:+32025904 MS.gene60884:CDS
TGGCTACCAATAGCACCTCA+TGG 0.618656 2.3:+32025787 MS.gene60884:CDS
AATACAAGTGAGTAATCAAG+AGG 0.619444 2.3:-32025718 None:intergenic
AGATATTGAATTGCAGAATG+TGG 0.619484 2.3:+32026878 MS.gene60884:CDS
AGTGGCATCATTGATCTTGG+TGG 0.625498 2.3:+32023971 MS.gene60884:CDS
AAGGTGCCAACTCGAACACC+GGG 0.628473 2.3:-32025982 None:intergenic
TCAACATTCAGCAAAGTTTG+GGG 0.632154 2.3:+32025523 MS.gene60884:CDS
GAAGGTGATGGATTTGCAAG+TGG 0.636338 2.3:+32023953 MS.gene60884:intron
GTTTGGACAACCAATGAAGG+TGG 0.636585 2.3:+32024031 MS.gene60884:CDS
AGTAGATTAATGCCAGCATG+TGG 0.637012 2.3:-32026690 None:intergenic
ATTGGTAGCCAAATATACAA+AGG 0.637296 2.3:-32025775 None:intergenic
TGACAACATGGCCTACAGCA+TGG 0.643727 2.3:-32025813 None:intergenic
ACAAGTGAGTAATCAAGAGG+TGG 0.647867 2.3:-32025715 None:intergenic
TGTAGTGAATGTGGCGAGAG+CGG 0.649291 2.3:-32025312 None:intergenic
ACGATTCACATTGCTCTGTG+TGG 0.653711 2.3:-32025888 None:intergenic
GCTTAGATGTGTAGTGAATG+TGG 0.662600 2.3:-32025321 None:intergenic
ATTTGATACATTCATCAGTG+AGG 0.666824 2.3:-32024381 None:intergenic
GATGTTAGACCAATCAAGAG+AGG 0.668053 2.3:+32024454 MS.gene60884:CDS
GGGAAAGCAGGTGAACATGT+TGG 0.668748 2.3:+32026477 MS.gene60884:CDS
TTAAGGGTAAGTAACTTCGA+TGG 0.678056 2.3:+32026519 MS.gene60884:CDS
AGGAGAGTCTTGAGTAACCG+TGG 0.681322 2.3:-32025755 None:intergenic
CATTCACTGATATTGCCATG+TGG 0.686069 2.3:+32026394 MS.gene60884:CDS
CATGTGAAACCAATGTATGG+AGG 0.726903 2.3:+32026369 MS.gene60884:CDS
CCTACAGCATGGTAGCCATG+AGG 0.740121 2.3:-32025802 None:intergenic
AGAGTCTTGAGTAACCGTGG+CGG 0.757224 2.3:-32025752 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTAAGTTCATATATT+AGG - chr2.3:32025190-32025209 None:intergenic 10.0%
!! ACCTTAAAATTTATATTCTA+TGG - chr2.3:32025054-32025073 None:intergenic 15.0%
!! AGAGTTTATAGATATTAAAT+TGG + chr2.3:32026455-32026474 MS.gene60884:CDS 15.0%
!! AGTTTATAGATATTAAATTG+GGG + chr2.3:32026457-32026476 MS.gene60884:CDS 15.0%
!! ATATATAGAATAAAGCAAAT+AGG - chr2.3:32023829-32023848 None:intergenic 15.0%
!! GAGTTTATAGATATTAAATT+GGG + chr2.3:32026456-32026475 MS.gene60884:CDS 15.0%
!!! ACAAATTTAAGTAATGATTT+TGG - chr2.3:32024814-32024833 None:intergenic 15.0%
!!! ATGGTTTTGAAAATATTATT+AGG + chr2.3:32026928-32026947 MS.gene60884:CDS 15.0%
!!! TCCATAGAATATAAATTTTA+AGG + chr2.3:32025050-32025069 MS.gene60884:intron 15.0%
!! AGTATTAAATTGCAACATAA+AGG + chr2.3:32025445-32025464 MS.gene60884:intron 20.0%
!! TAATAAAGGTAGAAATGTTA+TGG + chr2.3:32026746-32026765 MS.gene60884:CDS 20.0%
!! TGACTAGAATATATTACAAA+TGG - chr2.3:32025406-32025425 None:intergenic 20.0%
!! TTAAATATAATCACATGTGT+TGG - chr2.3:32023720-32023739 None:intergenic 20.0%
!! TTATATTCTATGGAAATCTT+GGG - chr2.3:32025044-32025063 None:intergenic 20.0%
!! TTTATATTCTATGGAAATCT+TGG - chr2.3:32025045-32025064 None:intergenic 20.0%
!!! AATTTTGGGATGATTTTTAT+TGG + chr2.3:32023915-32023934 MS.gene60884:intron 20.0%
!!! GTATTTTTCAAACAAACAAT+AGG - chr2.3:32024550-32024569 None:intergenic 20.0%
!!! TTTTTTAAAGCTTTGTTTGT+GGG - chr2.3:32024190-32024209 None:intergenic 20.0%
!!! TTTTTTTAAAGCTTTGTTTG+TGG - chr2.3:32024191-32024210 None:intergenic 20.0%
! AAAACCACTTGATTACAAAT+TGG + chr2.3:32024207-32024226 MS.gene60884:CDS 25.0%
! ACATTGCTTATTTAGGAAAT+AGG - chr2.3:32023869-32023888 None:intergenic 25.0%
! ATGCTTAACAAGAGTTTATA+AGG + chr2.3:32025117-32025136 MS.gene60884:intron 25.0%
! ATTAATCTACTAGGGAAAAA+TGG + chr2.3:32026702-32026721 MS.gene60884:CDS 25.0%
! GCTTTAATTCCTCTATTTAT+TGG - chr2.3:32025961-32025980 None:intergenic 25.0%
! GTTAACTACATTGCTTATTT+AGG - chr2.3:32023876-32023895 None:intergenic 25.0%
! TATAGATCCTTAGACAAATT+GGG - chr2.3:32024779-32024798 None:intergenic 25.0%
! TCAAACATGAAATTGATGAA+GGG - chr2.3:32026035-32026054 None:intergenic 25.0%
! TTATAGATCCTTAGACAAAT+TGG - chr2.3:32024780-32024799 None:intergenic 25.0%
! TTCAAACATGAAATTGATGA+AGG - chr2.3:32026036-32026055 None:intergenic 25.0%
! TTTCAACATTCAACAAAGTT+TGG + chr2.3:32024014-32024033 MS.gene60884:CDS 25.0%
!! TGGAATGGTTTTTCATAAAT+GGG + chr2.3:32024683-32024702 MS.gene60884:intron 25.0%
!! TTTCCATGTGAATTTGATTT+GGG - chr2.3:32025094-32025113 None:intergenic 25.0%
!! TTTTCCATGTGAATTTGATT+TGG - chr2.3:32025095-32025114 None:intergenic 25.0%
!!! AGCATGGTTGAATTATTTTA+GGG + chr2.3:32026827-32026846 MS.gene60884:CDS 25.0%
!!! ATCATTTTATTTGAAGGTGA+TGG + chr2.3:32023941-32023960 MS.gene60884:intron 25.0%
!!! GCATCAATTTGTTTTTTACT+AGG - chr2.3:32026087-32026106 None:intergenic 25.0%
!!! GTTTTGATTTTCCAACAAAA+AGG + chr2.3:32026628-32026647 MS.gene60884:CDS 25.0%
!!! TGGCTTATCATTTTATTTGA+AGG + chr2.3:32023935-32023954 MS.gene60884:intron 25.0%
AAGACTCTCCTTTGTATATT+TGG + chr2.3:32025767-32025786 MS.gene60884:CDS 30.0%
AAGGAAAAGAGTAATTGGAA+TGG + chr2.3:32026996-32027015 MS.gene60884:CDS 30.0%
AATACAAGTCAATGAAGCTT+TGG + chr2.3:32024401-32024420 MS.gene60884:CDS 30.0%
AATACAAGTGAGTAATCAAG+AGG - chr2.3:32025721-32025740 None:intergenic 30.0%
ACAAGAACATAACCAAAAAG+AGG - chr2.3:32025655-32025674 None:intergenic 30.0%
ACTTATGGATACTCTATGTT+AGG + chr2.3:32025604-32025623 MS.gene60884:CDS 30.0%
AGATATTGAATTGCAGAATG+TGG + chr2.3:32026878-32026897 MS.gene60884:CDS 30.0%
AGGTAGAAATGTTATGGATA+TGG + chr2.3:32026752-32026771 MS.gene60884:CDS 30.0%
ATACATGTATTTGCATCCTA+TGG + chr2.3:32024639-32024658 MS.gene60884:intron 30.0%
ATACTCTACCAAAAAAGAGA+TGG + chr2.3:32024709-32024728 MS.gene60884:intron 30.0%
ATAGATCCTTAGACAAATTG+GGG - chr2.3:32024778-32024797 None:intergenic 30.0%
ATAGGATGCAAATACATGTA+TGG - chr2.3:32024640-32024659 None:intergenic 30.0%
ATCAACATTCAGCAAAGTTT+GGG + chr2.3:32025522-32025541 MS.gene60884:CDS 30.0%
ATCACATAAATGATACCTTC+TGG - chr2.3:32025370-32025389 None:intergenic 30.0%
ATCACATACAAGGCTATATT+TGG + chr2.3:32024260-32024279 MS.gene60884:CDS 30.0%
ATTGTTTATGCTTCTTTGCA+TGG + chr2.3:32026654-32026673 MS.gene60884:CDS 30.0%
ATTTGATACATTCATCAGTG+AGG - chr2.3:32024384-32024403 None:intergenic 30.0%
CAAAACAAACATCACATACA+AGG + chr2.3:32024250-32024269 MS.gene60884:CDS 30.0%
CATACCAATTTGTAATCAAG+TGG - chr2.3:32024214-32024233 None:intergenic 30.0%
CCAAATTGAGACATTAATCA+AGG + chr2.3:32024606-32024625 MS.gene60884:intron 30.0%
CCTTGATTAATGTCTCAATT+TGG - chr2.3:32024609-32024628 None:intergenic 30.0%
CTAAAATAATTCAACCATGC+TGG - chr2.3:32026828-32026847 None:intergenic 30.0%
CTTCATCAGAATGTAAGTAT+AGG - chr2.3:32026134-32026153 None:intergenic 30.0%
GATATTTCAGGAAAGAGTAA+TGG + chr2.3:32025682-32025701 MS.gene60884:CDS 30.0%
GATGTTAGACCAATAAATAG+AGG + chr2.3:32025949-32025968 MS.gene60884:CDS 30.0%
GCAAGAGATGATACTAATAA+AGG + chr2.3:32026732-32026751 MS.gene60884:CDS 30.0%
GGAAGAAAATCTATTAGCAT+TGG - chr2.3:32024572-32024591 None:intergenic 30.0%
GGATATGGGAAAATATGTAT+TGG + chr2.3:32026767-32026786 MS.gene60884:CDS 30.0%
GGTAGAAATGTTATGGATAT+GGG + chr2.3:32026753-32026772 MS.gene60884:CDS 30.0%
TCAGAATGTAAGTATAGGAA+TGG - chr2.3:32026129-32026148 None:intergenic 30.0%
TCTAGATCAAAGTCAATATG+TGG - chr2.3:32026861-32026880 None:intergenic 30.0%
TCTGCAAACAAAAAAACATG+TGG - chr2.3:32025262-32025281 None:intergenic 30.0%
TGGTTTCTGCAGAATATTTA+GGG + chr2.3:32026787-32026806 MS.gene60884:CDS 30.0%
TTAAATGAGGTTTGAATGAG+TGG - chr2.3:32023595-32023614 None:intergenic 30.0%
TTGGTTTCTGCAGAATATTT+AGG + chr2.3:32026786-32026805 MS.gene60884:CDS 30.0%
! ATTGGTAGCCAAATATACAA+AGG - chr2.3:32025778-32025797 None:intergenic 30.0%
! CAAAATCGACTTATAAGATG+AGG - chr2.3:32025152-32025171 None:intergenic 30.0%
! CACATAATTTTGTCTAGTGA+AGG - chr2.3:32024349-32024368 None:intergenic 30.0%
! CAGCATGGTTGAATTATTTT+AGG + chr2.3:32026826-32026845 MS.gene60884:CDS 30.0%
! GGAATGGTTTTTCATAAATG+GGG + chr2.3:32024684-32024703 MS.gene60884:intron 30.0%
! GTGGAATGGTTTTTCATAAA+TGG + chr2.3:32024682-32024701 MS.gene60884:intron 30.0%
! GTTACAATTTTTGAACCAAC+AGG + chr2.3:32024070-32024089 MS.gene60884:CDS 30.0%
! TAAACAATAGGCCTTTTTGT+TGG - chr2.3:32026642-32026661 None:intergenic 30.0%
! TGCAAAGAAGCATAAACAAT+AGG - chr2.3:32026654-32026673 None:intergenic 30.0%
! TTCCAATTACTCTTTTCCTT+TGG - chr2.3:32026996-32027015 None:intergenic 30.0%
!! AATTTGGTGTAAATTAGGCA+TGG - chr2.3:32024593-32024612 None:intergenic 30.0%
!! GCATCTATGAGATTTGTTTT+CGG + chr2.3:32024988-32025007 MS.gene60884:intron 30.0%
!! GTCTCAATTTGGTGTAAATT+AGG - chr2.3:32024598-32024617 None:intergenic 30.0%
!! TGAATCGTCTACATTGATTT+GGG + chr2.3:32025903-32025922 MS.gene60884:CDS 30.0%
!! TTCCATGTGAATTTGATTTG+GGG - chr2.3:32025093-32025112 None:intergenic 30.0%
!!! GTTAATTGGTTTTTCTCCAA+CGG + chr2.3:32026171-32026190 MS.gene60884:CDS 30.0%
!!! GTTGAATTATTTTAGGGAGT+GGG + chr2.3:32026833-32026852 MS.gene60884:CDS 30.0%
!!! TCTGAAGGTTTCTTTGTTTT+AGG + chr2.3:32024097-32024116 MS.gene60884:CDS 30.0%
!!! TGGGTTTTTTATCCTTTCAA+TGG + chr2.3:32026414-32026433 MS.gene60884:CDS 30.0%
!!! TTGAATTATTTTAGGGAGTG+GGG + chr2.3:32026834-32026853 MS.gene60884:CDS 30.0%
AAAAACCAATTAACCGAGGA+AGG - chr2.3:32026165-32026184 None:intergenic 35.0%
AAAAACCATTCCACAGAAGA+TGG - chr2.3:32024676-32024695 None:intergenic 35.0%
AAACCTTCAGATAAACCTGT+TGG - chr2.3:32024088-32024107 None:intergenic 35.0%
AAAGTTTGGACAACCAATGA+AGG + chr2.3:32024028-32024047 MS.gene60884:CDS 35.0%
AACAAAGAGAGAGTCAAGAA+AGG + chr2.3:32026318-32026337 MS.gene60884:CDS 35.0%
AATAGAGGAATTAAAGCTCC+CGG + chr2.3:32025964-32025983 MS.gene60884:CDS 35.0%
AGTTTACCAAAAGAAGCCTT+GGG + chr2.3:32026951-32026970 MS.gene60884:CDS 35.0%
ATCTTGTTGCAGTGTTCATT+CGG - chr2.3:32024873-32024892 None:intergenic 35.0%
ATGCTGGCATTAATCTACTA+GGG + chr2.3:32026694-32026713 MS.gene60884:CDS 35.0%
ATTCACTGATATTGCCATGT+GGG + chr2.3:32026395-32026414 MS.gene60884:CDS 35.0%
ATTGTAACTCCAAGATCATC+TGG - chr2.3:32024058-32024077 None:intergenic 35.0%
CAAAACTACTAGCATCACTT+CGG - chr2.3:32026614-32026633 None:intergenic 35.0%
CATCAACATTCAGCAAAGTT+TGG + chr2.3:32025521-32025540 MS.gene60884:CDS 35.0%
CTTGTAGCAAAGGATATTTC+AGG + chr2.3:32025670-32025689 MS.gene60884:CDS 35.0%
GAAAGGATAAAAAACCCACA+TGG - chr2.3:32026412-32026431 None:intergenic 35.0%
GATATTAAATTGGGGAAAGC+AGG + chr2.3:32026465-32026484 MS.gene60884:CDS 35.0%
GCAAATAGGTGTAGAAATCT+AGG - chr2.3:32023815-32023834 None:intergenic 35.0%
GTAACTTCGATGGTCAATTA+TGG + chr2.3:32026529-32026548 MS.gene60884:CDS 35.0%
GTACATGTGAAACCAATGTA+TGG + chr2.3:32026366-32026385 MS.gene60884:CDS 35.0%
GTCCAAAGGAAAAGAGTAAT+TGG + chr2.3:32026991-32027010 MS.gene60884:CDS 35.0%
TACACTATGTGTTCATGCAA+GGG - chr2.3:32025230-32025249 None:intergenic 35.0%
TACCCCAAATCAAATTCACA+TGG + chr2.3:32025088-32025107 MS.gene60884:intron 35.0%
TAGGAAATAGGTATATCAGC+AGG - chr2.3:32023857-32023876 None:intergenic 35.0%
TCAACATTCAGCAAAGTTTG+GGG + chr2.3:32025523-32025542 MS.gene60884:CDS 35.0%
TGAAGAATACTTACCTTCCT+CGG + chr2.3:32026149-32026168 MS.gene60884:CDS 35.0%
TGGTTATGTTCTTGTAGCAA+AGG + chr2.3:32025660-32025679 MS.gene60884:CDS 35.0%
TTAAGGGTAAGTAACTTCGA+TGG + chr2.3:32026519-32026538 MS.gene60884:CDS 35.0%
TTACACTATGTGTTCATGCA+AGG - chr2.3:32025231-32025250 None:intergenic 35.0%
TTTGGAGAACAGAAAACAAG+AGG + chr2.3:32024419-32024438 MS.gene60884:CDS 35.0%
! GATATACCAGTTTTTCCAAC+AGG + chr2.3:32023625-32023644 MS.gene60884:CDS 35.0%
! GGAAGGTTTATCTTGTGTTT+TGG - chr2.3:32025839-32025858 None:intergenic 35.0%
! GGAGCATTACTATACAAGAA+AGG + chr2.3:32026192-32026211 MS.gene60884:CDS 35.0%
! GTGAATCGTCTACATTGATT+TGG + chr2.3:32025902-32025921 MS.gene60884:CDS 35.0%
! TCTTTGTTTGCATTATCAGC+AGG - chr2.3:32026306-32026325 None:intergenic 35.0%
! TTAGCACCATTTTGTGCTAT+GGG - chr2.3:32026237-32026256 None:intergenic 35.0%
! TTTTATCCTTTCAATGGACC+TGG + chr2.3:32026420-32026439 MS.gene60884:CDS 35.0%
!! ACATTGATTTGGGGATCAAA+CGG + chr2.3:32025913-32025932 MS.gene60884:CDS 35.0%
!! GAAGCTAAAGGTGTTTATGT+AGG + chr2.3:32024481-32024500 MS.gene60884:intron 35.0%
!! GAATCGTCTACATTGATTTG+GGG + chr2.3:32025904-32025923 MS.gene60884:CDS 35.0%
!! GAGTGGACTTTTCTTTTTCT+TGG - chr2.3:32023578-32023597 None:intergenic 35.0%
!!! GGTTGAATTATTTTAGGGAG+TGG + chr2.3:32026832-32026851 MS.gene60884:CDS 35.0%
!!! TAACGACACCATCTCTTTTT+TGG - chr2.3:32024720-32024739 None:intergenic 35.0%
AAACCATCTCTTAACTTCCC+AGG - chr2.3:32026915-32026934 None:intergenic 40.0%
AACCTTCCCCAATTTGTCTA+AGG + chr2.3:32024769-32024788 MS.gene60884:intron 40.0%
ACAACAAACCTCTTCATGGT+TGG + chr2.3:32024137-32024156 MS.gene60884:CDS 40.0%
ACAAGTGAGTAATCAAGAGG+TGG - chr2.3:32025718-32025737 None:intergenic 40.0%
ACACTATGTGTTCATGCAAG+GGG - chr2.3:32025229-32025248 None:intergenic 40.0%
ACCTTTGTTGCTCAAATTGG+TGG + chr2.3:32026000-32026019 MS.gene60884:CDS 40.0%
ACTTACCTTCCTCGGTTAAT+TGG + chr2.3:32026157-32026176 MS.gene60884:CDS 40.0%
AGTAGATTAATGCCAGCATG+TGG - chr2.3:32026693-32026712 None:intergenic 40.0%
ATCAGCAGGAAGCTTAAATG+AGG - chr2.3:32023608-32023627 None:intergenic 40.0%
ATCATGTGACTCATACCTGT+TGG - chr2.3:32023643-32023662 None:intergenic 40.0%
ATCCTTAGACAAATTGGGGA+AGG - chr2.3:32024774-32024793 None:intergenic 40.0%
CAACAAACCTCTTCATGGTT+GGG + chr2.3:32024138-32024157 MS.gene60884:CDS 40.0%
CAATAGGAACCATCAGTGTT+AGG - chr2.3:32026273-32026292 None:intergenic 40.0%
CATGCTGGCATTAATCTACT+AGG + chr2.3:32026693-32026712 MS.gene60884:CDS 40.0%
CATGTGAAACCAATGTATGG+AGG + chr2.3:32026369-32026388 MS.gene60884:CDS 40.0%
CATTCACTGATATTGCCATG+TGG + chr2.3:32026394-32026413 MS.gene60884:CDS 40.0%
CCAAACAACAAACCTCTTCA+TGG + chr2.3:32024133-32024152 MS.gene60884:CDS 40.0%
CCTTTGTTGCTCAAATTGGT+GGG + chr2.3:32026001-32026020 MS.gene60884:CDS 40.0%
CTAACACTGATGGTTCCTAT+TGG + chr2.3:32026271-32026290 MS.gene60884:CDS 40.0%
CTACTAGGGAAAAATGGAGT+AGG + chr2.3:32026708-32026727 MS.gene60884:CDS 40.0%
CTCAACACAATGCTGGTAAA+TGG + chr2.3:32026565-32026584 MS.gene60884:CDS 40.0%
CTCATACCTGTTGGAAAAAC+TGG - chr2.3:32023634-32023653 None:intergenic 40.0%
CTTGTATAGTAATGCTCCGT+TGG - chr2.3:32026190-32026209 None:intergenic 40.0%
GAACCAACAGGTTTATCTGA+AGG + chr2.3:32024082-32024101 MS.gene60884:CDS 40.0%
GAGTTTACCAAAAGAAGCCT+TGG + chr2.3:32026950-32026969 MS.gene60884:CDS 40.0%
GATGACTGTGTTTGCAAAAC+TGG - chr2.3:32025473-32025492 None:intergenic 40.0%
GATGTTAGACCAATCAAGAG+AGG + chr2.3:32024454-32024473 MS.gene60884:CDS 40.0%
GCAAACACAGTCATCAATCT+AGG + chr2.3:32025478-32025497 MS.gene60884:CDS 40.0%
GCTTAGATGTGTAGTGAATG+TGG - chr2.3:32025324-32025343 None:intergenic 40.0%
GCTTCAGTTTATGAACCAAC+AGG + chr2.3:32025577-32025596 MS.gene60884:CDS 40.0%
GGAAAAACTGGTATATCAGC+AGG - chr2.3:32023622-32023641 None:intergenic 40.0%
GGACATGACACTAACACATT+GGG - chr2.3:32023773-32023792 None:intergenic 40.0%
GGAGAAAAACCAATTAACCG+AGG - chr2.3:32026169-32026188 None:intergenic 40.0%
GTGAATGTTCCTCCATACAT+TGG - chr2.3:32026381-32026400 None:intergenic 40.0%
TAAGTACCTTCCATCTTCTG+TGG + chr2.3:32024663-32024682 MS.gene60884:intron 40.0%
TACTTCTCTCAACACAATGC+TGG + chr2.3:32026558-32026577 MS.gene60884:CDS 40.0%
TATCCATAAGTCAGTCCTGT+TGG - chr2.3:32025595-32025614 None:intergenic 40.0%
TCTGACACACATGACTTTGT+TGG - chr2.3:32025863-32025882 None:intergenic 40.0%
TGAATTGCAGAATGTGGCTA+AGG + chr2.3:32026884-32026903 MS.gene60884:CDS 40.0%
TGTTTGCATTATCAGCAGGT+AGG - chr2.3:32026302-32026321 None:intergenic 40.0%
TTCCTGTTTCACAACCTTCT+TGG + chr2.3:32025344-32025363 MS.gene60884:CDS 40.0%
TTTGTCGTCTAAGTGAGAGT+CGG - chr2.3:32024936-32024955 None:intergenic 40.0%
! ATAGGAATGGAGCGTTAACT+TGG - chr2.3:32026116-32026135 None:intergenic 40.0%
! ATTGGGAGCATGTGACATTA+AGG + chr2.3:32026502-32026521 MS.gene60884:CDS 40.0%
! GTTAGCACCATTTTGTGCTA+TGG - chr2.3:32026238-32026257 None:intergenic 40.0%
! TGTTGTTCGTTTTCTTGCTG+TGG - chr2.3:32024910-32024929 None:intergenic 40.0%
! TTGGGAGCATGTGACATTAA+GGG + chr2.3:32026503-32026522 MS.gene60884:CDS 40.0%
! TTTTCCTTCCACATTGAGCA+AGG - chr2.3:32024513-32024532 None:intergenic 40.0%
!! AAGAGAGGTGTTGAAGCTAA+AGG + chr2.3:32024469-32024488 MS.gene60884:CDS 40.0%
!! AGGTGAACATGTTGGTGATT+GGG + chr2.3:32026485-32026504 MS.gene60884:CDS 40.0%
!! CCATGAAGAGGTTTGTTGTT+TGG - chr2.3:32024136-32024155 None:intergenic 40.0%
!! GAAGAGGTTTGTTGTTTGGT+TGG - chr2.3:32024132-32024151 None:intergenic 40.0%
!! GATGGAAGGTACTTATCCAT+AGG - chr2.3:32024658-32024677 None:intergenic 40.0%
!! TGAGATTTGTTTTCGGCTTC+TGG + chr2.3:32024995-32025014 MS.gene60884:intron 40.0%
!! TTTGATCCCGTTGGTTCAAT+TGG - chr2.3:32024751-32024770 None:intergenic 40.0%
!!! CTTGTGTTTTGGTGACAACA+TGG - chr2.3:32025828-32025847 None:intergenic 40.0%
AACTCTACTTTAGCCCTTCC+AGG - chr2.3:32026441-32026460 None:intergenic 45.0%
ACAAGAACCCAACCATGAAG+AGG - chr2.3:32024148-32024167 None:intergenic 45.0%
ACCATTCCACAGAAGATGGA+AGG - chr2.3:32024672-32024691 None:intergenic 45.0%
ACCTTCCATCTTCTGTGGAA+TGG + chr2.3:32024668-32024687 MS.gene60884:intron 45.0%
ACGATTCACATTGCTCTGTG+TGG - chr2.3:32025891-32025910 None:intergenic 45.0%
AGTGGCATCATTGATCTTGG+TGG + chr2.3:32023971-32023990 MS.gene60884:CDS 45.0%
ATCATCTGGTCCACCTTCAT+TGG - chr2.3:32024044-32024063 None:intergenic 45.0%
ATCCTTTCAATGGACCTGGA+AGG + chr2.3:32026424-32026443 MS.gene60884:CDS 45.0%
ATGATACCTTCTGGCCAAGA+AGG - chr2.3:32025361-32025380 None:intergenic 45.0%
CAGTGTTAGGATCTTGAGGA+AGG - chr2.3:32026260-32026279 None:intergenic 45.0%
CATGCATCATATCCACATGC+TGG + chr2.3:32026678-32026697 MS.gene60884:CDS 45.0%
CCAACAACTTTGTAGCCATC+AGG - chr2.3:32024298-32024317 None:intergenic 45.0%
CCATCAGTGTTAGGATCTTG+AGG - chr2.3:32026264-32026283 None:intergenic 45.0%
CCCACCAATTTGAGCAACAA+AGG - chr2.3:32026004-32026023 None:intergenic 45.0%
CCTCAAGATCCTAACACTGA+TGG + chr2.3:32026261-32026280 MS.gene60884:CDS 45.0%
CCTGATGGCTACAAAGTTGT+TGG + chr2.3:32024295-32024314 MS.gene60884:CDS 45.0%
CCTGTTCCCATAGCACAAAA+TGG + chr2.3:32026228-32026247 MS.gene60884:CDS 45.0%
CGGACATGACACTAACACAT+TGG - chr2.3:32023774-32023793 None:intergenic 45.0%
CTGAAGTTAAAGAGCCAGCA+TGG + chr2.3:32026811-32026830 MS.gene60884:CDS 45.0%
CTTCATGGTTGGGTTCTTGT+AGG + chr2.3:32024148-32024167 MS.gene60884:CDS 45.0%
CTTGGGAAGTAGTGACAACA+TGG - chr2.3:32024321-32024340 None:intergenic 45.0%
GACACACATGACTTTGTTGG+TGG - chr2.3:32025860-32025879 None:intergenic 45.0%
GCAAGTGGCATCATTGATCT+TGG + chr2.3:32023968-32023987 MS.gene60884:CDS 45.0%
GCTTCAACACCTCTCTTGAT+TGG - chr2.3:32024466-32024485 None:intergenic 45.0%
GGAACCATTGACACCAACAT+CGG - chr2.3:32023794-32023813 None:intergenic 45.0%
GGCACCTTTGTTGCTCAAAT+TGG + chr2.3:32025997-32026016 MS.gene60884:CDS 45.0%
GTCTAGTGAAGGCTTCTCTT+GGG - chr2.3:32024338-32024357 None:intergenic 45.0%
GTGTTCATGCAAGGGGAATA+AGG - chr2.3:32025222-32025241 None:intergenic 45.0%
GTTAGTGTCATGTCCGATGT+TGG + chr2.3:32023778-32023797 MS.gene60884:intron 45.0%
GTTGCAGTGTTCATTCGGTT+TGG - chr2.3:32024868-32024887 None:intergenic 45.0%
GTTTGGACAACCAATGAAGG+TGG + chr2.3:32024031-32024050 MS.gene60884:CDS 45.0%
TCCTTTCAATGGACCTGGAA+GGG + chr2.3:32026425-32026444 MS.gene60884:CDS 45.0%
TGCGAGTCCAATTGAACCAA+CGG + chr2.3:32024741-32024760 MS.gene60884:intron 45.0%
TGTCTAGTGAAGGCTTCTCT+TGG - chr2.3:32024339-32024358 None:intergenic 45.0%
TTGCCTGGGAAGTTAAGAGA+TGG + chr2.3:32026909-32026928 MS.gene60884:CDS 45.0%
! ACACGTTCAGTCTGAAGTGT+TGG - chr2.3:32023682-32023701 None:intergenic 45.0%
! CACATGACTTTGTTGGTGGA+AGG - chr2.3:32025856-32025875 None:intergenic 45.0%
! CCATTTTGTGCTATGGGAAC+AGG - chr2.3:32026231-32026250 None:intergenic 45.0%
! CTCTTTTCCTTTGGACCTGT+TGG - chr2.3:32026987-32027006 None:intergenic 45.0%
! GAACCAACAGGACTGACTTA+TGG + chr2.3:32025589-32025608 MS.gene60884:CDS 45.0%
!! ACCAAAAAGAGGCTTGCTGT+TGG - chr2.3:32025644-32025663 None:intergenic 45.0%
!! ATGTCCGATGTTGGTGTCAA+TGG + chr2.3:32023787-32023806 MS.gene60884:intron 45.0%
!! CAGGTGAACATGTTGGTGAT+TGG + chr2.3:32026484-32026503 MS.gene60884:CDS 45.0%
!! CCAAAAAGAGGCTTGCTGTT+GGG - chr2.3:32025643-32025662 None:intergenic 45.0%
!! GAAGGTGATGGATTTGCAAG+TGG + chr2.3:32023953-32023972 MS.gene60884:intron 45.0%
!!! ATTTATTTTTTTTGAATTTT+GGG + chr2.3:32023901-32023920 MS.gene60884:intron 5.0%
!!! TATTTATTTTTTTTGAATTT+TGG + chr2.3:32023900-32023919 MS.gene60884:intron 5.0%
AAAGAAGCCTTGGGAGAGGA+AGG + chr2.3:32026960-32026979 MS.gene60884:CDS 50.0%
ACCAAAAGAAGCCTTGGGAG+AGG + chr2.3:32026956-32026975 MS.gene60884:CDS 50.0%
AGAGTCTTGAGTAACCGTGG+CGG - chr2.3:32025755-32025774 None:intergenic 50.0%
AGCAGGTAGGTCAAGCCAAT+AGG - chr2.3:32026289-32026308 None:intergenic 50.0%
AGGAGAGTCTTGAGTAACCG+TGG - chr2.3:32025758-32025777 None:intergenic 50.0%
ATTGAACACTGCATCCGCCA+CGG + chr2.3:32025738-32025757 MS.gene60884:CDS 50.0%
CCTTCCACATTGAGCAAGGA+AGG - chr2.3:32024509-32024528 None:intergenic 50.0%
CCTTCCTTGCTCAATGTGGA+AGG + chr2.3:32024506-32024525 MS.gene60884:intron 50.0%
GAAGGTGGACCAGATGATCT+TGG + chr2.3:32024046-32024065 MS.gene60884:CDS 50.0%
GCGAGTCCAATTGAACCAAC+GGG + chr2.3:32024742-32024761 MS.gene60884:intron 50.0%
GGAACCTTCCTTGCTCAATG+TGG + chr2.3:32024502-32024521 MS.gene60884:intron 50.0%
GGCATCATTGATCTTGGTGG+TGG + chr2.3:32023974-32023993 MS.gene60884:CDS 50.0%
GGCCAAGAAGGTTGTGAAAC+AGG - chr2.3:32025349-32025368 None:intergenic 50.0%
GGGAAAGCAGGTGAACATGT+TGG + chr2.3:32026477-32026496 MS.gene60884:CDS 50.0%
TCACAACCTTCTTGGCCAGA+AGG + chr2.3:32025352-32025371 MS.gene60884:CDS 50.0%
TGACAACATGGCCTACAGCA+TGG - chr2.3:32025816-32025835 None:intergenic 50.0%
TGGCTACCAATAGCACCTCA+TGG + chr2.3:32025787-32025806 MS.gene60884:CDS 50.0%
TGGTTACCAGTAGCACCTGA+TGG + chr2.3:32024280-32024299 MS.gene60884:CDS 50.0%
TGTAGTGAATGTGGCGAGAG+CGG - chr2.3:32025315-32025334 None:intergenic 50.0%
! CCCAACAGCAAGCCTCTTTT+TGG + chr2.3:32025640-32025659 MS.gene60884:CDS 50.0%
! TCCTCTCCCAAGGCTTCTTT+TGG - chr2.3:32026960-32026979 None:intergenic 50.0%
! TGGTAGCCATGAGGTGCTAT+TGG - chr2.3:32025796-32025815 None:intergenic 50.0%
! TTGTAGCCATCAGGTGCTAC+TGG - chr2.3:32024289-32024308 None:intergenic 50.0%
!! AAAGAGGCTTGCTGTTGGGT+TGG - chr2.3:32025639-32025658 None:intergenic 50.0%
!! AAAGGTGCCAACTCGAACAC+CGG - chr2.3:32025986-32026005 None:intergenic 50.0%
AAAGCTCCCGGTGTTCGAGT+TGG + chr2.3:32025976-32025995 MS.gene60884:CDS 55.0%
CCTACAGCATGGTAGCCATG+AGG - chr2.3:32025805-32025824 None:intergenic 55.0%
CCTCATGGCTACCATGCTGT+AGG + chr2.3:32025802-32025821 MS.gene60884:CDS 55.0%
GCCCTTCCAGGTCCATTGAA+AGG - chr2.3:32026429-32026448 None:intergenic 55.0%
GGAAGGACCAACAGGTCCAA+AGG + chr2.3:32026977-32026996 MS.gene60884:CDS 55.0%
GTAGTGAATGTGGCGAGAGC+GGG - chr2.3:32025314-32025333 None:intergenic 55.0%
TGGGGAAGGTTTGATCCCGT+TGG - chr2.3:32024760-32024779 None:intergenic 55.0%
TGTTGGTCCTTCCTCTCCCA+AGG - chr2.3:32026970-32026989 None:intergenic 55.0%
TTGGGAGAGGAAGGACCAAC+AGG + chr2.3:32026969-32026988 MS.gene60884:CDS 55.0%
! AAAGTTTGGGGCGCCTACGA+CGG + chr2.3:32025535-32025554 MS.gene60884:CDS 55.0%
! AAGGTGCCAACTCGAACACC+GGG - chr2.3:32025985-32026004 None:intergenic 55.0%
!! AATGTGGCTAAGGCGTTGCC+TGG + chr2.3:32026894-32026913 MS.gene60884:CDS 55.0%
!! ATGTGGCTAAGGCGTTGCCT+GGG + chr2.3:32026895-32026914 MS.gene60884:CDS 55.0%
! ACTGAAGCGCCTCGATTGCC+CGG - chr2.3:32025565-32025584 None:intergenic 60.0%
ATTGCCCGGTCCACCGTCGT+AGG - chr2.3:32025551-32025570 None:intergenic 65.0%
GACGGTGGACCGGGCAATCG+AGG + chr2.3:32025553-32025572 MS.gene60884:CDS 70.0%
! GTTTGGGGCGCCTACGACGG+TGG + chr2.3:32025538-32025557 MS.gene60884:CDS 70.0%
GGCGCCTACGACGGTGGACC+GGG + chr2.3:32025544-32025563 MS.gene60884:CDS 75.0%
GGGCGCCTACGACGGTGGAC+CGG + chr2.3:32025543-32025562 MS.gene60884:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 32023553 32027031 32023553 ID=MS.gene60884
chr2.3 mRNA 32023553 32027031 32023553 ID=MS.gene60884.t1;Parent=MS.gene60884
chr2.3 exon 32023553 32023646 32023553 ID=MS.gene60884.t1.exon1;Parent=MS.gene60884.t1
chr2.3 CDS 32023553 32023646 32023553 ID=cds.MS.gene60884.t1;Parent=MS.gene60884.t1
chr2.3 exon 32023957 32024490 32023957 ID=MS.gene60884.t1.exon2;Parent=MS.gene60884.t1
chr2.3 CDS 32023957 32024490 32023957 ID=cds.MS.gene60884.t1;Parent=MS.gene60884.t1
chr2.3 exon 32025281 32025373 32025281 ID=MS.gene60884.t1.exon3;Parent=MS.gene60884.t1
chr2.3 CDS 32025281 32025373 32025281 ID=cds.MS.gene60884.t1;Parent=MS.gene60884.t1
chr2.3 exon 32025467 32027031 32025467 ID=MS.gene60884.t1.exon4;Parent=MS.gene60884.t1
chr2.3 CDS 32025467 32027031 32025467 ID=cds.MS.gene60884.t1;Parent=MS.gene60884.t1
Gene Sequence

>MS.gene60884

ATGTTGAGTTCTCTTAGCAATTCCAAGAAAAAGAAAAGTCCACTCATTCAAACCTCATTTAAGCTTCCTGCTGATATACCAGTTTTTCCAACAGGTATGAGTCACATGATTTGTAGATATTTAGCACCAACACTTCAGACTGAACGTGTATTTTGTGTTTGACACCAACACATGTGATTATATTTAATTATTCTATTTTCATAAACTATTAATTGATCCCAATGTGTTAGTGTCATGTCCGATGTTGGTGTCAATGGTTCCTAGATTTCTACACCTATTTGCTTTATTCTATATATATACACCTGCTGATATACCTATTTCCTAAATAAGCAATGTAGTTAACATTTTATTTATTTTTTTTGAATTTTGGGATGATTTTTATTGGCTTATCATTTTATTTGAAGGTGATGGATTTGCAAGTGGCATCATTGATCTTGGTGGTGGTTTATTAGTGTCACAAATTTCAACATTCAACAAAGTTTGGACAACCAATGAAGGTGGACCAGATGATCTTGGAGTTACAATTTTTGAACCAACAGGTTTATCTGAAGGTTTCTTTGTTTTAGGATACTACTGCCAACCAAACAACAAACCTCTTCATGGTTGGGTTCTTGTAGGTAAAGATAACTCTTCACCCACAAACAAAGCTTTAAAAAAACCACTTGATTACAAATTGGTATGTAACATAAAGTCTCTACAAAACAAACATCACATACAAGGCTATATTTGGTTACCAGTAGCACCTGATGGCTACAAAGTTGTTGGCCATGTTGTCACTACTTCCCAAGAGAAGCCTTCACTAGACAAAATTATGTGTGTTAGATCAGACCTCACTGATGAATGTATCAAATACAAGTCAATGAAGCTTTGGAGAACAGAAAACAAGAGGTTCAATGTTTATGATGTTAGACCAATCAAGAGAGGTGTTGAAGCTAAAGGTGTTTATGTAGGAACCTTCCTTGCTCAATGTGGAAGGAAAAATTCTAAAACTTTGCCTATTGTTTGTTTGAAAAATACCAATGCTAATAGATTTTCTTCCATGCCTAATTTACACCAAATTGAGACATTAATCAAGGCTTACTCTCCATACATGTATTTGCATCCTATGGATAAGTACCTTCCATCTTCTGTGGAATGGTTTTTCATAAATGGGGCAATACTCTACCAAAAAAGAGATGGTGTCGTTAATGCGAGTCCAATTGAACCAACGGGATCAAACCTTCCCCAATTTGTCTAAGGATCTATAAAATTGAAGACGCCAAAATCATTACTTAAATTTGTAATGTGCTCTAAACGAAGAAAATCTATCAATCCAAACCGAATGAACACTGCAACAAGATGAAAAATTAAACTGCCACAGCAAGAAAACGAACAACACAACCGACTCTCACTTAGACGACAAAATAAAAGACAATTCTTAGCGAGCTAATTCTATGCATCTATGAGATTTGTTTTCGGCTTCTGGAAAAGCTAATTCTTAGCGAGCTTCCCAAGATTTCCATAGAATATAAATTTTAAGGTGTCTCATAGATACTTACCCCAAATCAAATTCACATGGAAAATTATGCTTAACAAGAGTTTATAAGGAATAACACTCCTCATCTTATAAGTCGATTTTGTAAGTATTTGTTAGACCTAATATATGAACTTAATAATTAACAAGAGACCTTATTCCCCTTGCATGAACACATAGTGTAATTGTCTTACCACATGTTTTTTTGTTTGCAGAGAATAAGAATGATTTCAAGAAAAAGCGATCCCGCTCTCGCCACATTCACTACACATCTAAGCTTCCTGTTTCACAACCTTCTTGGCCAGAAGGTATCATTTATGTGATTTCAATTTTCTGTCCATTTGTAATATATTCTAGTCAATGTAAGCTAACTATAGCAAGTATTAAATTGCAACATAAAGGTACCAGTTTTGCAAACACAGTCATCAATCTAGGTGAGCTGCATGTGATGCAAGCATCAACATTCAGCAAAGTTTGGGGCGCCTACGACGGTGGACCGGGCAATCGAGGCGCTTCAGTTTATGAACCAACAGGACTGACTTATGGATACTCTATGTTAGGCAGTTACAGCCAACCCAACAGCAAGCCTCTTTTTGGTTATGTTCTTGTAGCAAAGGATATTTCAGGAAAGAGTAATGGCACTTTAAAGCCACCTCTTGATTACTCACTTGTATTGAACACTGCATCCGCCACGGTTACTCAAGACTCTCCTTTGTATATTTGGCTACCAATAGCACCTCATGGCTACCATGCTGTAGGCCATGTTGTCACCAAAACACAAGATAAACCTTCCACCAACAAAGTCATGTGTGTCAGATCAGACCACACAGAGCAATGTGAATCGTCTACATTGATTTGGGGATCAAACGGCTTAAATTTTTACGATGTTAGACCAATAAATAGAGGAATTAAAGCTCCCGGTGTTCGAGTTGGCACCTTTGTTGCTCAAATTGGTGGGAATATTAACCCTTCATCAATTTCATGTTTGAAGAATGTTGATTCTATTACACAAATCATGCCTAGTAAAAAACAAATTGATGCACTACTCCAAGTTAACGCTCCATTCCTATACTTACATTCTGATGAAGAATACTTACCTTCCTCGGTTAATTGGTTTTTCTCCAACGGAGCATTACTATACAAGAAAGGTCAAGAATCAAATCCTGTTCCCATAGCACAAAATGGTGCTAACCTTCCTCAAGATCCTAACACTGATGGTTCCTATTGGCTTGACCTACCTGCTGATAATGCAAACAAAGAGAGAGTCAAGAAAGGTAATCTACATAGTGCAGAATCATATGTACATGTGAAACCAATGTATGGAGGAACATTCACTGATATTGCCATGTGGGTTTTTTATCCTTTCAATGGACCTGGAAGGGCTAAAGTAGAGTTTATAGATATTAAATTGGGGAAAGCAGGTGAACATGTTGGTGATTGGGAGCATGTGACATTAAGGGTAAGTAACTTCGATGGTCAATTATGGCATATCTACTTCTCTCAACACAATGCTGGTAAATGGATTGATTCATCTCAACTTGAGTTCCGAAGTGATGCTAGTAGTTTTGATTTTCCAACAAAAAGGCCTATTGTTTATGCTTCTTTGCATGGTCATGCATCATATCCACATGCTGGCATTAATCTACTAGGGAAAAATGGAGTAGGTGCAAGAGATGATACTAATAAAGGTAGAAATGTTATGGATATGGGAAAATATGTATTGGTTTCTGCAGAATATTTAGGGTCTGAAGTTAAAGAGCCAGCATGGTTGAATTATTTTAGGGAGTGGGGTCCACATATTGACTTTGATCTAGATATTGAATTGCAGAATGTGGCTAAGGCGTTGCCTGGGAAGTTAAGAGATGGTTTTGAAAATATTATTAGGAGTTTACCAAAAGAAGCCTTGGGAGAGGAAGGACCAACAGGTCCAAAGGAAAAGAGTAATTGGAATGGTGATGAAGTTTGA
Protein sequence

>MS.gene60884.t1

MLSSLSNSKKKKSPLIQTSFKLPADIPVFPTGDGFASGIIDLGGGLLVSQISTFNKVWTTNEGGPDDLGVTIFEPTGLSEGFFVLGYYCQPNNKPLHGWVLVGKDNSSPTNKALKKPLDYKLVCNIKSLQNKHHIQGYIWLPVAPDGYKVVGHVVTTSQEKPSLDKIMCVRSDLTDECIKYKSMKLWRTENKRFNVYDVRPIKRGVEAKENKNDFKKKRSRSRHIHYTSKLPVSQPSWPEGTSFANTVINLGELHVMQASTFSKVWGAYDGGPGNRGASVYEPTGLTYGYSMLGSYSQPNSKPLFGYVLVAKDISGKSNGTLKPPLDYSLVLNTASATVTQDSPLYIWLPIAPHGYHAVGHVVTKTQDKPSTNKVMCVRSDHTEQCESSTLIWGSNGLNFYDVRPINRGIKAPGVRVGTFVAQIGGNINPSSISCLKNVDSITQIMPSKKQIDALLQVNAPFLYLHSDEEYLPSSVNWFFSNGALLYKKGQESNPVPIAQNGANLPQDPNTDGSYWLDLPADNANKERVKKGNLHSAESYVHVKPMYGGTFTDIAMWVFYPFNGPGRAKVEFIDIKLGKAGEHVGDWEHVTLRVSNFDGQLWHIYFSQHNAGKWIDSSQLEFRSDASSFDFPTKRPIVYASLHGHASYPHAGINLLGKNGVGARDDTNKGRNVMDMGKYVLVSAEYLGSEVKEPAWLNYFREWGPHIDFDLDIELQNVAKALPGKLRDGFENIIRSLPKEALGEEGPTGPKEKSNWNGDEV