AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000191

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000191.t1 MTR_2g062380 95.446 505 23 0 1 505 56 560 0.0 998
MS.gene000191.t1 MTR_2g062370 83.597 506 82 1 1 505 85 590 0.0 894
MS.gene000191.t1 MTR_2g062410 82.376 505 84 2 2 505 86 586 0.0 872
MS.gene000191.t1 MTR_2g062430 72.673 505 128 2 2 505 58 553 0.0 778
MS.gene000191.t1 MTR_4g131710 71.542 506 141 2 3 505 81 586 0.0 764
MS.gene000191.t1 MTR_2g067640 54.086 514 205 8 2 505 49 541 0.0 559
MS.gene000191.t1 MTR_2g062340 51.462 513 193 9 2 504 50 516 5.17e-172 495
MS.gene000191.t1 MTR_1g080370 45.962 520 251 9 2 504 52 558 2.69e-155 454
MS.gene000191.t1 MTR_1g080410 45.962 520 251 9 2 504 52 558 2.69e-155 454
MS.gene000191.t1 MTR_5g007860 43.774 530 261 10 1 504 87 605 1.75e-146 433
MS.gene000191.t1 MTR_4g077620 43.321 524 263 12 2 504 57 567 2.28e-137 408
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000191.t1 AT2G44260 55.556 504 213 4 2 504 89 582 0.0 584
MS.gene000191.t1 AT2G44260 55.357 504 214 4 2 504 59 552 0.0 584
MS.gene000191.t1 AT2G44230 53.150 508 220 6 2 505 49 542 0.0 543
MS.gene000191.t1 AT3G01870 48.031 508 245 6 1 504 91 583 9.24e-171 494
MS.gene000191.t1 AT3G01880 48.515 505 243 6 4 504 98 589 8.07e-168 487
MS.gene000191.t1 AT5G18490 44.402 518 257 9 1 505 53 552 3.76e-151 443
MS.gene000191.t1 AT3G04350 43.429 525 265 9 1 505 32 544 1.29e-146 431
MS.gene000191.t1 AT3G04350 43.429 525 265 9 1 505 54 566 6.37e-146 430
MS.gene000191.t1 AT1G04090 42.264 530 269 12 1 504 52 570 1.44e-139 414
MS.gene000191.t1 AT5G43950 42.004 519 278 8 2 504 53 564 3.21e-138 410

Find 131 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACTCTCCTTTGTATATT+TGG 0.127143 2.1:+33581141 MS.gene000191:CDS
CAGCATGGTTGAATTATTTC+AGG 0.166486 2.1:+33582200 MS.gene000191:CDS
ACTTACCTTCCTCGGTTAAT+TGG 0.215430 2.1:+33581531 MS.gene000191:CDS
CATCAACATTTAGCAAAGTT+TGG 0.229268 2.1:+33580895 MS.gene000191:CDS
AGAGTTTATAGATATTAAAT+TGG 0.239669 2.1:+33581829 MS.gene000191:CDS
TTGGTTTCTGCAGAATATTT+AGG 0.246737 2.1:+33582160 MS.gene000191:CDS
CTTGTAGCAAAGGATATTTC+AGG 0.255851 2.1:+33581044 MS.gene000191:CDS
TCCTCTCCCAAGGCTTCTTT+TGG 0.261151 2.1:-33582331 None:intergenic
CTCAACACAATGCTGGTAAA+TGG 0.273775 2.1:+33581939 MS.gene000191:CDS
AGGTAGAAATGTTATGGATA+TGG 0.277907 2.1:+33582126 MS.gene000191:CDS
GTGAATCGTCTACATTGATT+TGG 0.279504 2.1:+33581276 MS.gene000191:CDS
ATTGGGAGCATGTGACATTA+AGG 0.285039 2.1:+33581876 MS.gene000191:CDS
GTCCAAAGGAAAAGGGTAAT+TGG 0.292083 2.1:+33582365 MS.gene000191:CDS
TGGTTTCTGCAGAATATTTA+GGG 0.297210 2.1:+33582161 MS.gene000191:CDS
ATCAACATTTAGCAAAGTTT+GGG 0.302175 2.1:+33580896 MS.gene000191:CDS
TGAATCGTCTACATTGATTT+GGG 0.313780 2.1:+33581277 MS.gene000191:CDS
AACTCTACTTTAGCTCTTCC+AGG 0.339774 2.1:-33581812 None:intergenic
GTAACTTCGATGGTCAATTA+TGG 0.344009 2.1:+33581903 MS.gene000191:CDS
GCTTTAGTTCCTCTATTGAT+TGG 0.347877 2.1:-33581332 None:intergenic
CTGACACACATAACTTTGTT+CGG 0.352301 2.1:-33581233 None:intergenic
CATGCTGGCATTAATCTACT+AGG 0.355903 2.1:+33582067 MS.gene000191:CDS
GGAAGGTTTGTCTTGTGTTT+TGG 0.365408 2.1:-33581210 None:intergenic
TTCTTCAAACATGAAATTGA+TGG 0.368521 2.1:-33581410 None:intergenic
GAACCAACAGGACTGACTTA+TGG 0.368553 2.1:+33580963 MS.gene000191:CDS
TGACACACATAACTTTGTTC+GGG 0.371288 2.1:-33581232 None:intergenic
TAATAAAGGTAGAAATGTTA+TGG 0.389081 2.1:+33582120 MS.gene000191:CDS
CTAACACTGATGGTTCCTAT+TGG 0.400945 2.1:+33581645 MS.gene000191:CDS
GGTACCTTCGTTGCTCAAAA+TGG 0.404119 2.1:+33581371 MS.gene000191:CDS
ATTGTTTATGCTTCTTTGCA+TGG 0.404302 2.1:+33582028 MS.gene000191:CDS
CCTGTTCCAATAGCACAAAA+TGG 0.406019 2.1:+33581602 MS.gene000191:CDS
CAATAGGAACCATCAGTGTT+AGG 0.411818 2.1:-33581644 None:intergenic
GAGTTTATAGATATTAAATT+GGG 0.415665 2.1:+33581830 MS.gene000191:CDS
GCAAGAGATGATACTAATAA+AGG 0.425735 2.1:+33582106 MS.gene000191:CDS
GGTAGAAATGTTATGGATAT+GGG 0.435541 2.1:+33582127 MS.gene000191:CDS
GGATATGGGAAAATATGTAT+TGG 0.435630 2.1:+33582141 MS.gene000191:CDS
GGGCGCCTACGAAGGTGGAC+CGG 0.436973 2.1:+33580917 MS.gene000191:CDS
ACCAACAGGTCCAAAGGAAA+AGG 0.437204 2.1:+33582357 MS.gene000191:CDS
CTTCATCGGAATGTAAGTAT+AGG 0.445447 2.1:-33581505 None:intergenic
GATATTAAATTGGGAAAAGC+AGG 0.451140 2.1:+33581839 MS.gene000191:CDS
ACTTATGGATACTCTATGTT+AGG 0.451687 2.1:+33580978 MS.gene000191:CDS
ACATTGATTTGGGGATCAAA+AGG 0.454612 2.1:+33581287 MS.gene000191:CDS
AATGTGGCTAAGGCATTGCC+TGG 0.460752 2.1:+33582268 MS.gene000191:CDS
AATACTAGTGAGTAATCAAC+AGG 0.461578 2.1:-33581092 None:intergenic
GATATTTCAGGAAAGACTAA+TGG 0.462884 2.1:+33581056 MS.gene000191:CDS
AGAAGGTAGGTCAAGCCAAT+AGG 0.465352 2.1:-33581660 None:intergenic
TGTTGGTCCTTCCTCTCCCA+AGG 0.465771 2.1:-33582341 None:intergenic
CTTGTATAGTAATGCTCCGT+TGG 0.470906 2.1:-33581561 None:intergenic
TGCAAAGAAGCATAAACAAT+AGG 0.471114 2.1:-33582025 None:intergenic
CAGGTGAACATGTTGGTGAT+TGG 0.472172 2.1:+33581858 MS.gene000191:CDS
GTTGAATTATTTCAGGGAGT+GGG 0.475428 2.1:+33582207 MS.gene000191:CDS
GGCGCCTACGAAGGTGGACC+GGG 0.477150 2.1:+33580918 MS.gene000191:CDS
ATAGGAATGGAGCGTTAACT+TGG 0.477931 2.1:-33581487 None:intergenic
TTGGGAGAGGAAGGACCAAC+AGG 0.483726 2.1:+33582343 MS.gene000191:CDS
GGTTGAATTATTTCAGGGAG+TGG 0.486043 2.1:+33582206 MS.gene000191:CDS
TGGTAGCCATGAGGTGCTAT+TGG 0.489916 2.1:-33581167 None:intergenic
CAAAACTACTAGCATCACTT+TGG 0.490875 2.1:-33581985 None:intergenic
ACTGAGGCGCCTCGATTGCC+CGG 0.492573 2.1:-33580936 None:intergenic
GAGTTTACCAAAAGAAGCCT+TGG 0.494113 2.1:+33582324 MS.gene000191:CDS
GCCTCAGTTTATGAACCAAC+AGG 0.503883 2.1:+33580951 MS.gene000191:CDS
TGGATATGTTCTTGTAGCAA+AGG 0.506215 2.1:+33581034 MS.gene000191:CDS
AGGTGAACATGTTGGTGATT+GGG 0.508342 2.1:+33581859 MS.gene000191:CDS
CTGAAATAATTCAACCATGC+TGG 0.508539 2.1:-33582199 None:intergenic
AGCATGGTTGAATTATTTCA+GGG 0.512489 2.1:+33582201 MS.gene000191:CDS
GCTCTTCCAGGTCCATTGAA+AGG 0.515609 2.1:-33581800 None:intergenic
CACATAACTTTGTTCGGGGA+AGG 0.517408 2.1:-33581227 None:intergenic
AGTTTACCAAAAGAAGCCTT+GGG 0.519013 2.1:+33582325 MS.gene000191:CDS
TGAAGAATACTTACCTTCCT+CGG 0.523409 2.1:+33581523 MS.gene000191:CDS
TTGGGAGCATGTGACATTAA+GGG 0.526297 2.1:+33581877 MS.gene000191:CDS
TCTAGATCAAAGTCAATATG+TGG 0.527060 2.1:-33582232 None:intergenic
GAAGGTACCAACACGAACAC+CGG 0.527971 2.1:-33581357 None:intergenic
AAACCATCTCTTAACTTCCC+AGG 0.534968 2.1:-33582286 None:intergenic
CCAACAGGTCCAAAGGAAAA+GGG 0.535094 2.1:+33582358 MS.gene000191:CDS
TTCAAACATGAAATTGATGG+AGG 0.536359 2.1:-33581407 None:intergenic
GTGAATGTTCCTCCATACAT+TGG 0.538131 2.1:-33581752 None:intergenic
AAAGAGGCTTGCTGTTGGGT+TGG 0.538892 2.1:-33581010 None:intergenic
TGTTTGCATTATCAGAAGGT+AGG 0.539120 2.1:-33581673 None:intergenic
TACTTCTCTCAACACAATGC+TGG 0.544284 2.1:+33581932 MS.gene000191:CDS
GTACATGTGAAACCAATGTA+TGG 0.546199 2.1:+33581740 MS.gene000191:CDS
GGAAGGACCAACAGGTCCAA+AGG 0.546506 2.1:+33582351 MS.gene000191:CDS
ACCAAAAGAAGCCTTGGGAG+AGG 0.547085 2.1:+33582330 MS.gene000191:CDS
AAAGAAGCCTTGGGAGAGGA+AGG 0.551794 2.1:+33582334 MS.gene000191:CDS
TGAATTGCAGAATGTGGCTA+AGG 0.555864 2.1:+33582258 MS.gene000191:CDS
ACCTTCGTTGCTCAAAATGG+TGG 0.556539 2.1:+33581374 MS.gene000191:CDS
TCGGAATGTAAGTATAGGAA+TGG 0.556685 2.1:-33581500 None:intergenic
TTGAATTATTTCAGGGAGTG+GGG 0.560005 2.1:+33582208 MS.gene000191:CDS
ATTGCCCGGTCCACCTTCGT+AGG 0.564907 2.1:-33580922 None:intergenic
ATTCACTGATATTGCCATGT+GGG 0.565127 2.1:+33581769 MS.gene000191:CDS
AAGGAAAAGGGTAATTGGAA+TGG 0.567477 2.1:+33582370 MS.gene000191:CDS
AATAGAGGAACTAAAGCTCC+CGG 0.568897 2.1:+33581338 MS.gene000191:CDS
AAATGGAAATAACCTACCTC+AGG 0.569559 2.1:+33581619 MS.gene000191:CDS
AAGGTACCAACACGAACACC+GGG 0.570465 2.1:-33581356 None:intergenic
AACAAAGAGAGAGTCAAGAA+AGG 0.571578 2.1:+33581692 MS.gene000191:CDS
TCTTTGTTTGCATTATCAGA+AGG 0.572050 2.1:-33581677 None:intergenic
TATCCATAAGTCAGTCCTGT+TGG 0.574072 2.1:-33580966 None:intergenic
ATGCTGGCATTAATCTACTA+GGG 0.576721 2.1:+33582068 MS.gene000191:CDS
GTTTGGGGCGCCTACGAAGG+TGG 0.577121 2.1:+33580912 MS.gene000191:CDS
CATGCATCATATCCACATGC+TGG 0.577243 2.1:+33582052 MS.gene000191:CDS
GAAGGTGGACCGGGCAATCG+AGG 0.579936 2.1:+33580927 MS.gene000191:CDS
GGAGCATTACTATACAAGAA+AGG 0.581086 2.1:+33581566 MS.gene000191:CDS
GGAAGGTAAGTATTCTTCAT+CGG 0.581670 2.1:-33581519 None:intergenic
TCAAACATGAAATTGATGGA+GGG 0.588797 2.1:-33581406 None:intergenic
CCTCAGGATCCTAACACTGA+TGG 0.591487 2.1:+33581635 MS.gene000191:CDS
CTGAAGTTAAAGAGCCAGCA+TGG 0.591737 2.1:+33582185 MS.gene000191:CDS
TTGCCTGGGAAGTTAAGAGA+TGG 0.593085 2.1:+33582283 MS.gene000191:CDS
AAAGTTTGGGGCGCCTACGA+AGG 0.597769 2.1:+33580909 MS.gene000191:CDS
CCTCATGGCTACCATGCTGT+AGG 0.600980 2.1:+33581176 MS.gene000191:CDS
CCTTCGTTGCTCAAAATGGT+GGG 0.609529 2.1:+33581375 MS.gene000191:CDS
GACGTTAGACCAATCAATAG+AGG 0.612860 2.1:+33581323 MS.gene000191:CDS
CAAAGGAGAGTCTTGAGTAA+CGG 0.617891 2.1:-33581132 None:intergenic
GAATCGTCTACATTGATTTG+GGG 0.618231 2.1:+33581278 MS.gene000191:CDS
TGGCTACCAATAGCACCTCA+TGG 0.618656 2.1:+33581161 MS.gene000191:CDS
CCATCAGTGTTAGGATCCTG+AGG 0.619098 2.1:-33581635 None:intergenic
AGATATTGAATTGCAGAATG+TGG 0.619484 2.1:+33582252 MS.gene000191:CDS
CAGTGTTAGGATCCTGAGGT+AGG 0.628219 2.1:-33581631 None:intergenic
AGAGTCTTGAGTAACGGTGG+CGG 0.628693 2.1:-33581126 None:intergenic
AGTAGATTAATGCCAGCATG+TGG 0.637012 2.1:-33582064 None:intergenic
ATTGGTAGCCAAATATACAA+AGG 0.637296 2.1:-33581149 None:intergenic
ATGTGGCTAAGGCATTGCCT+GGG 0.639158 2.1:+33582269 MS.gene000191:CDS
AAAGCTCCCGGTGTTCGTGT+TGG 0.641608 2.1:+33581350 MS.gene000191:CDS
GACACACATAACTTTGTTCG+GGG 0.647317 2.1:-33581231 None:intergenic
TCCTGTTGGTTCATAAACTG+AGG 0.647497 2.1:-33580952 None:intergenic
TCAACATTTAGCAAAGTTTG+GGG 0.649526 2.1:+33580897 MS.gene000191:CDS
TGACAACAAGGCCTACAGCA+TGG 0.652162 2.1:-33581187 None:intergenic
GGAAAAGCAGGTGAACATGT+TGG 0.666452 2.1:+33581851 MS.gene000191:CDS
TTAAGGGTAAGTAACTTCGA+TGG 0.678056 2.1:+33581893 MS.gene000191:CDS
CATTCACTGATATTGCCATG+TGG 0.686069 2.1:+33581768 MS.gene000191:CDS
AGGAGAGTCTTGAGTAACGG+TGG 0.690919 2.1:-33581129 None:intergenic
ACTAGTGAGTAATCAACAGG+TGG 0.691530 2.1:-33581089 None:intergenic
ACGATTCACATTGCTCTGTG+AGG 0.692137 2.1:-33581262 None:intergenic
CATGTGAAACCAATGTATGG+AGG 0.726903 2.1:+33581743 MS.gene000191:CDS
CCTACAGCATGGTAGCCATG+AGG 0.740121 2.1:-33581176 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGTTTATAGATATTAAAT+TGG + chr2.1:33581829-33581848 MS.gene000191:CDS 15.0%
!! GAGTTTATAGATATTAAATT+GGG + chr2.1:33581830-33581849 MS.gene000191:CDS 15.0%
!! TAATAAAGGTAGAAATGTTA+TGG + chr2.1:33582120-33582139 MS.gene000191:CDS 20.0%
!!! ATGGTTTTGAAAGTATTATT+AGG + chr2.1:33582302-33582321 MS.gene000191:CDS 20.0%
! ATCAACATTTAGCAAAGTTT+GGG + chr2.1:33580896-33580915 MS.gene000191:CDS 25.0%
! ATTAATCTACTAGGGAAAAA+TGG + chr2.1:33582076-33582095 MS.gene000191:CDS 25.0%
! TTCTTCAAACATGAAATTGA+TGG - chr2.1:33581413-33581432 None:intergenic 25.0%
!! TAAACAATAGGTCTTTTTGT+TGG - chr2.1:33582016-33582035 None:intergenic 25.0%
!! TTATTTCCATTTTGTGCTAT+TGG - chr2.1:33581611-33581630 None:intergenic 25.0%
!!! GCATCAATTTGTTTTTTACT+AGG - chr2.1:33581461-33581480 None:intergenic 25.0%
AAGACTCTCCTTTGTATATT+TGG + chr2.1:33581141-33581160 MS.gene000191:CDS 30.0%
AATACTAGTGAGTAATCAAC+AGG - chr2.1:33581095-33581114 None:intergenic 30.0%
ACAAGAACATATCCAAAAAG+AGG - chr2.1:33581029-33581048 None:intergenic 30.0%
ACTTATGGATACTCTATGTT+AGG + chr2.1:33580978-33580997 MS.gene000191:CDS 30.0%
AGATATTGAATTGCAGAATG+TGG + chr2.1:33582252-33582271 MS.gene000191:CDS 30.0%
AGCATGGTTGAATTATTTCA+GGG + chr2.1:33582201-33582220 MS.gene000191:CDS 30.0%
AGGTAGAAATGTTATGGATA+TGG + chr2.1:33582126-33582145 MS.gene000191:CDS 30.0%
ATTGTTTATGCTTCTTTGCA+TGG + chr2.1:33582028-33582047 MS.gene000191:CDS 30.0%
CATCAACATTTAGCAAAGTT+TGG + chr2.1:33580895-33580914 MS.gene000191:CDS 30.0%
GATATTAAATTGGGAAAAGC+AGG + chr2.1:33581839-33581858 MS.gene000191:CDS 30.0%
GATATTTCAGGAAAGACTAA+TGG + chr2.1:33581056-33581075 MS.gene000191:CDS 30.0%
GCAAGAGATGATACTAATAA+AGG + chr2.1:33582106-33582125 MS.gene000191:CDS 30.0%
GGATATGGGAAAATATGTAT+TGG + chr2.1:33582141-33582160 MS.gene000191:CDS 30.0%
GGTAGAAATGTTATGGATAT+GGG + chr2.1:33582127-33582146 MS.gene000191:CDS 30.0%
TCAAACATGAAATTGATGGA+GGG - chr2.1:33581409-33581428 None:intergenic 30.0%
TCAACATTTAGCAAAGTTTG+GGG + chr2.1:33580897-33580916 MS.gene000191:CDS 30.0%
TCTAGATCAAAGTCAATATG+TGG - chr2.1:33582235-33582254 None:intergenic 30.0%
TGGTTTCTGCAGAATATTTA+GGG + chr2.1:33582161-33582180 MS.gene000191:CDS 30.0%
TTCAAACATGAAATTGATGG+AGG - chr2.1:33581410-33581429 None:intergenic 30.0%
TTGGTTTCTGCAGAATATTT+AGG + chr2.1:33582160-33582179 MS.gene000191:CDS 30.0%
! ATTGGTAGCCAAATATACAA+AGG - chr2.1:33581152-33581171 None:intergenic 30.0%
! TCTTTGTTTGCATTATCAGA+AGG - chr2.1:33581680-33581699 None:intergenic 30.0%
! TGCAAAGAAGCATAAACAAT+AGG - chr2.1:33582028-33582047 None:intergenic 30.0%
!! TGAATCGTCTACATTGATTT+GGG + chr2.1:33581277-33581296 MS.gene000191:CDS 30.0%
!!! GTTAATTGGTTTTTCTCCAA+CGG + chr2.1:33581545-33581564 MS.gene000191:CDS 30.0%
!!! TGGGTTTTTTATCCTTTCAA+TGG + chr2.1:33581788-33581807 MS.gene000191:CDS 30.0%
AAAAACCAATTAACCGAGGA+AGG - chr2.1:33581539-33581558 None:intergenic 35.0%
AAATGGAAATAACCTACCTC+AGG + chr2.1:33581619-33581638 MS.gene000191:CDS 35.0%
AACAAAGAGAGAGTCAAGAA+AGG + chr2.1:33581692-33581711 MS.gene000191:CDS 35.0%
AAGGAAAAGGGTAATTGGAA+TGG + chr2.1:33582370-33582389 MS.gene000191:CDS 35.0%
AGTTTACCAAAAGAAGCCTT+GGG + chr2.1:33582325-33582344 MS.gene000191:CDS 35.0%
ATGCTGGCATTAATCTACTA+GGG + chr2.1:33582068-33582087 MS.gene000191:CDS 35.0%
ATTCACTGATATTGCCATGT+GGG + chr2.1:33581769-33581788 MS.gene000191:CDS 35.0%
CAAAACTACTAGCATCACTT+TGG - chr2.1:33581988-33582007 None:intergenic 35.0%
CAGCATGGTTGAATTATTTC+AGG + chr2.1:33582200-33582219 MS.gene000191:CDS 35.0%
CTGAAATAATTCAACCATGC+TGG - chr2.1:33582202-33582221 None:intergenic 35.0%
CTGACACACATAACTTTGTT+CGG - chr2.1:33581236-33581255 None:intergenic 35.0%
CTTCATCGGAATGTAAGTAT+AGG - chr2.1:33581508-33581527 None:intergenic 35.0%
CTTGTAGCAAAGGATATTTC+AGG + chr2.1:33581044-33581063 MS.gene000191:CDS 35.0%
GAAAGGATAAAAAACCCACA+TGG - chr2.1:33581786-33581805 None:intergenic 35.0%
GGAAGGTAAGTATTCTTCAT+CGG - chr2.1:33581522-33581541 None:intergenic 35.0%
GTAACTTCGATGGTCAATTA+TGG + chr2.1:33581903-33581922 MS.gene000191:CDS 35.0%
GTACATGTGAAACCAATGTA+TGG + chr2.1:33581740-33581759 MS.gene000191:CDS 35.0%
GTTGAATTATTTCAGGGAGT+GGG + chr2.1:33582207-33582226 MS.gene000191:CDS 35.0%
TCGGAATGTAAGTATAGGAA+TGG - chr2.1:33581503-33581522 None:intergenic 35.0%
TGAAGAATACTTACCTTCCT+CGG + chr2.1:33581523-33581542 MS.gene000191:CDS 35.0%
TGACACACATAACTTTGTTC+GGG - chr2.1:33581235-33581254 None:intergenic 35.0%
TGGATATGTTCTTGTAGCAA+AGG + chr2.1:33581034-33581053 MS.gene000191:CDS 35.0%
TGTTTGCATTATCAGAAGGT+AGG - chr2.1:33581676-33581695 None:intergenic 35.0%
TTAAGGGTAAGTAACTTCGA+TGG + chr2.1:33581893-33581912 MS.gene000191:CDS 35.0%
TTGAATTATTTCAGGGAGTG+GGG + chr2.1:33582208-33582227 MS.gene000191:CDS 35.0%
! GCTTTAGTTCCTCTATTGAT+TGG - chr2.1:33581335-33581354 None:intergenic 35.0%
! GGAGCATTACTATACAAGAA+AGG + chr2.1:33581566-33581585 MS.gene000191:CDS 35.0%
! GTGAATCGTCTACATTGATT+TGG + chr2.1:33581276-33581295 MS.gene000191:CDS 35.0%
! TTCCAATTACCCTTTTCCTT+TGG - chr2.1:33582370-33582389 None:intergenic 35.0%
! TTTTATCCTTTCAATGGACC+TGG + chr2.1:33581794-33581813 MS.gene000191:CDS 35.0%
!! ACATTGATTTGGGGATCAAA+AGG + chr2.1:33581287-33581306 MS.gene000191:CDS 35.0%
!! GAATCGTCTACATTGATTTG+GGG + chr2.1:33581278-33581297 MS.gene000191:CDS 35.0%
AAACCATCTCTTAACTTCCC+AGG - chr2.1:33582289-33582308 None:intergenic 40.0%
AATAGAGGAACTAAAGCTCC+CGG + chr2.1:33581338-33581357 MS.gene000191:CDS 40.0%
ACTAGTGAGTAATCAACAGG+TGG - chr2.1:33581092-33581111 None:intergenic 40.0%
ACTTACCTTCCTCGGTTAAT+TGG + chr2.1:33581531-33581550 MS.gene000191:CDS 40.0%
AGTAGATTAATGCCAGCATG+TGG - chr2.1:33582067-33582086 None:intergenic 40.0%
CAAAGGAGAGTCTTGAGTAA+CGG - chr2.1:33581135-33581154 None:intergenic 40.0%
CAATAGGAACCATCAGTGTT+AGG - chr2.1:33581647-33581666 None:intergenic 40.0%
CATGCTGGCATTAATCTACT+AGG + chr2.1:33582067-33582086 MS.gene000191:CDS 40.0%
CATGTGAAACCAATGTATGG+AGG + chr2.1:33581743-33581762 MS.gene000191:CDS 40.0%
CATTCACTGATATTGCCATG+TGG + chr2.1:33581768-33581787 MS.gene000191:CDS 40.0%
CCTGTTCCAATAGCACAAAA+TGG + chr2.1:33581602-33581621 MS.gene000191:CDS 40.0%
CTAACACTGATGGTTCCTAT+TGG + chr2.1:33581645-33581664 MS.gene000191:CDS 40.0%
CTACTAGGGAAAAATGGAGT+AGG + chr2.1:33582082-33582101 MS.gene000191:CDS 40.0%
CTCAACACAATGCTGGTAAA+TGG + chr2.1:33581939-33581958 MS.gene000191:CDS 40.0%
CTTGTATAGTAATGCTCCGT+TGG - chr2.1:33581564-33581583 None:intergenic 40.0%
GACACACATAACTTTGTTCG+GGG - chr2.1:33581234-33581253 None:intergenic 40.0%
GACGTTAGACCAATCAATAG+AGG + chr2.1:33581323-33581342 MS.gene000191:CDS 40.0%
GAGTTTACCAAAAGAAGCCT+TGG + chr2.1:33582324-33582343 MS.gene000191:CDS 40.0%
GGAGAAAAACCAATTAACCG+AGG - chr2.1:33581543-33581562 None:intergenic 40.0%
GGTTGAATTATTTCAGGGAG+TGG + chr2.1:33582206-33582225 MS.gene000191:CDS 40.0%
GTCCAAAGGAAAAGGGTAAT+TGG + chr2.1:33582365-33582384 MS.gene000191:CDS 40.0%
GTGAATGTTCCTCCATACAT+TGG - chr2.1:33581755-33581774 None:intergenic 40.0%
TACTTCTCTCAACACAATGC+TGG + chr2.1:33581932-33581951 MS.gene000191:CDS 40.0%
TATCCATAAGTCAGTCCTGT+TGG - chr2.1:33580969-33580988 None:intergenic 40.0%
TGAATTGCAGAATGTGGCTA+AGG + chr2.1:33582258-33582277 MS.gene000191:CDS 40.0%
! ATAGGAATGGAGCGTTAACT+TGG - chr2.1:33581490-33581509 None:intergenic 40.0%
! ATTGGGAGCATGTGACATTA+AGG + chr2.1:33581876-33581895 MS.gene000191:CDS 40.0%
! GGAAGGTTTGTCTTGTGTTT+TGG - chr2.1:33581213-33581232 None:intergenic 40.0%
! TTGGGAGCATGTGACATTAA+GGG + chr2.1:33581877-33581896 MS.gene000191:CDS 40.0%
!! AACTCTACTTTAGCTCTTCC+AGG - chr2.1:33581815-33581834 None:intergenic 40.0%
!! AGGTGAACATGTTGGTGATT+GGG + chr2.1:33581859-33581878 MS.gene000191:CDS 40.0%
!! CCATTTTGTGCTATTGGAAC+AGG - chr2.1:33581605-33581624 None:intergenic 40.0%
!! TCCTGTTGGTTCATAAACTG+AGG - chr2.1:33580955-33580974 None:intergenic 40.0%
!!! CTTGTGTTTTGGTGACAACA+AGG - chr2.1:33581202-33581221 None:intergenic 40.0%
ACCAACAGGTCCAAAGGAAA+AGG + chr2.1:33582357-33582376 MS.gene000191:CDS 45.0%
ACCTTCGTTGCTCAAAATGG+TGG + chr2.1:33581374-33581393 MS.gene000191:CDS 45.0%
ACGATTCACATTGCTCTGTG+AGG - chr2.1:33581265-33581284 None:intergenic 45.0%
AGAAGGTAGGTCAAGCCAAT+AGG - chr2.1:33581663-33581682 None:intergenic 45.0%
CACATAACTTTGTTCGGGGA+AGG - chr2.1:33581230-33581249 None:intergenic 45.0%
CATGCATCATATCCACATGC+TGG + chr2.1:33582052-33582071 MS.gene000191:CDS 45.0%
CCAACAGGTCCAAAGGAAAA+GGG + chr2.1:33582358-33582377 MS.gene000191:CDS 45.0%
CCTTCGTTGCTCAAAATGGT+GGG + chr2.1:33581375-33581394 MS.gene000191:CDS 45.0%
CTGAAGTTAAAGAGCCAGCA+TGG + chr2.1:33582185-33582204 MS.gene000191:CDS 45.0%
GCCTCAGTTTATGAACCAAC+AGG + chr2.1:33580951-33580970 MS.gene000191:CDS 45.0%
GGAAAAGCAGGTGAACATGT+TGG + chr2.1:33581851-33581870 MS.gene000191:CDS 45.0%
GGTACCTTCGTTGCTCAAAA+TGG + chr2.1:33581371-33581390 MS.gene000191:CDS 45.0%
TTGCCTGGGAAGTTAAGAGA+TGG + chr2.1:33582283-33582302 MS.gene000191:CDS 45.0%
! GAACCAACAGGACTGACTTA+TGG + chr2.1:33580963-33580982 MS.gene000191:CDS 45.0%
!! CAGGTGAACATGTTGGTGAT+TGG + chr2.1:33581858-33581877 MS.gene000191:CDS 45.0%
!! CCAAAAAGAGGCTTGCTGTT+GGG - chr2.1:33581017-33581036 None:intergenic 45.0%
!! TCCAAAAAGAGGCTTGCTGT+TGG - chr2.1:33581018-33581037 None:intergenic 45.0%
AAAGAAGCCTTGGGAGAGGA+AGG + chr2.1:33582334-33582353 MS.gene000191:CDS 50.0%
ACCAAAAGAAGCCTTGGGAG+AGG + chr2.1:33582330-33582349 MS.gene000191:CDS 50.0%
AGAGTCTTGAGTAACGGTGG+CGG - chr2.1:33581129-33581148 None:intergenic 50.0%
AGGAGAGTCTTGAGTAACGG+TGG - chr2.1:33581132-33581151 None:intergenic 50.0%
CAGTGTTAGGATCCTGAGGT+AGG - chr2.1:33581634-33581653 None:intergenic 50.0%
CCATCAGTGTTAGGATCCTG+AGG - chr2.1:33581638-33581657 None:intergenic 50.0%
CCTCAGGATCCTAACACTGA+TGG + chr2.1:33581635-33581654 MS.gene000191:CDS 50.0%
GCTCTTCCAGGTCCATTGAA+AGG - chr2.1:33581803-33581822 None:intergenic 50.0%
TGACAACAAGGCCTACAGCA+TGG - chr2.1:33581190-33581209 None:intergenic 50.0%
TGGCTACCAATAGCACCTCA+TGG + chr2.1:33581161-33581180 MS.gene000191:CDS 50.0%
! AAGGTACCAACACGAACACC+GGG - chr2.1:33581359-33581378 None:intergenic 50.0%
! CCCAACAGCAAGCCTCTTTT+TGG + chr2.1:33581014-33581033 MS.gene000191:CDS 50.0%
! CCCACCATTTTGAGCAACGA+AGG - chr2.1:33581378-33581397 None:intergenic 50.0%
! CCCTTTTCCTTTGGACCTGT+TGG - chr2.1:33582361-33582380 None:intergenic 50.0%
! TCCTCTCCCAAGGCTTCTTT+TGG - chr2.1:33582334-33582353 None:intergenic 50.0%
! TGGTAGCCATGAGGTGCTAT+TGG - chr2.1:33581170-33581189 None:intergenic 50.0%
!! AAAGAGGCTTGCTGTTGGGT+TGG - chr2.1:33581013-33581032 None:intergenic 50.0%
!! AATGTGGCTAAGGCATTGCC+TGG + chr2.1:33582268-33582287 MS.gene000191:CDS 50.0%
!! ATGTGGCTAAGGCATTGCCT+GGG + chr2.1:33582269-33582288 MS.gene000191:CDS 50.0%
!! GAAGGTACCAACACGAACAC+CGG - chr2.1:33581360-33581379 None:intergenic 50.0%
AAAGCTCCCGGTGTTCGTGT+TGG + chr2.1:33581350-33581369 MS.gene000191:CDS 55.0%
CCTACAGCATGGTAGCCATG+AGG - chr2.1:33581179-33581198 None:intergenic 55.0%
CCTCATGGCTACCATGCTGT+AGG + chr2.1:33581176-33581195 MS.gene000191:CDS 55.0%
GGAAGGACCAACAGGTCCAA+AGG + chr2.1:33582351-33582370 MS.gene000191:CDS 55.0%
TGTTGGTCCTTCCTCTCCCA+AGG - chr2.1:33582344-33582363 None:intergenic 55.0%
TTGGGAGAGGAAGGACCAAC+AGG + chr2.1:33582343-33582362 MS.gene000191:CDS 55.0%
! AAAGTTTGGGGCGCCTACGA+AGG + chr2.1:33580909-33580928 MS.gene000191:CDS 55.0%
ATTGCCCGGTCCACCTTCGT+AGG - chr2.1:33580925-33580944 None:intergenic 60.0%
GAAGGTGGACCGGGCAATCG+AGG + chr2.1:33580927-33580946 MS.gene000191:CDS 65.0%
! ACTGAGGCGCCTCGATTGCC+CGG - chr2.1:33580939-33580958 None:intergenic 65.0%
! GTTTGGGGCGCCTACGAAGG+TGG + chr2.1:33580912-33580931 MS.gene000191:CDS 65.0%
GGCGCCTACGAAGGTGGACC+GGG + chr2.1:33580918-33580937 MS.gene000191:CDS 70.0%
GGGCGCCTACGAAGGTGGAC+CGG + chr2.1:33580917-33580936 MS.gene000191:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 33580888 33582405 33580888 ID=MS.gene000191
chr2.1 mRNA 33580888 33582405 33580888 ID=MS.gene000191.t1;Parent=MS.gene000191
chr2.1 exon 33580888 33582405 33580888 ID=MS.gene000191.t1.exon1;Parent=MS.gene000191.t1
chr2.1 CDS 33580888 33582405 33580888 ID=cds.MS.gene000191.t1;Parent=MS.gene000191.t1
Gene Sequence

>MS.gene000191

ATGCAAGCATCAACATTTAGCAAAGTTTGGGGCGCCTACGAAGGTGGACCGGGCAATCGAGGCGCCTCAGTTTATGAACCAACAGGACTGACTTATGGATACTCTATGTTAGGCAGTTACAGCCAACCCAACAGCAAGCCTCTTTTTGGATATGTTCTTGTAGCAAAGGATATTTCAGGAAAGACTAATGGCACTTTAAAGCCACCTGTTGATTACTCACTAGTATTGAACACTGCATCCGCCACCGTTACTCAAGACTCTCCTTTGTATATTTGGCTACCAATAGCACCTCATGGCTACCATGCTGTAGGCCTTGTTGTCACCAAAACACAAGACAAACCTTCCCCGAACAAAGTTATGTGTGTCAGATCAGACCTCACAGAGCAATGTGAATCGTCTACATTGATTTGGGGATCAAAAGGCTTAAATTTCTACGACGTTAGACCAATCAATAGAGGAACTAAAGCTCCCGGTGTTCGTGTTGGTACCTTCGTTGCTCAAAATGGTGGGAATAGTAACCCTCCATCAATTTCATGTTTGAAGAATATTGATTCTATTACACAAATCATGCCTAGTAAAAAACAAATTGATGCACTACTCCAAGTTAACGCTCCATTCCTATACTTACATTCCGATGAAGAATACTTACCTTCCTCGGTTAATTGGTTTTTCTCCAACGGAGCATTACTATACAAGAAAGGTCATGAATCAAATCCTGTTCCAATAGCACAAAATGGAAATAACCTACCTCAGGATCCTAACACTGATGGTTCCTATTGGCTTGACCTACCTTCTGATAATGCAAACAAAGAGAGAGTCAAGAAAGGTAATCTACAAAGTGCAGAATCATATGTACATGTGAAACCAATGTATGGAGGAACATTCACTGATATTGCCATGTGGGTTTTTTATCCTTTCAATGGACCTGGAAGAGCTAAAGTAGAGTTTATAGATATTAAATTGGGAAAAGCAGGTGAACATGTTGGTGATTGGGAGCATGTGACATTAAGGGTAAGTAACTTCGATGGTCAATTATGGCATATCTACTTCTCTCAACACAATGCTGGTAAATGGATTGATTCATCTCAACTTGAGTTCCAAAGTGATGCTAGTAGTTTTGATTTTCCAACAAAAAGACCTATTGTTTATGCTTCTTTGCATGGTCATGCATCATATCCACATGCTGGCATTAATCTACTAGGGAAAAATGGAGTAGGTGCAAGAGATGATACTAATAAAGGTAGAAATGTTATGGATATGGGAAAATATGTATTGGTTTCTGCAGAATATTTAGGGTCTGAAGTTAAAGAGCCAGCATGGTTGAATTATTTCAGGGAGTGGGGTCCACATATTGACTTTGATCTAGATATTGAATTGCAGAATGTGGCTAAGGCATTGCCTGGGAAGTTAAGAGATGGTTTTGAAAGTATTATTAGGAGTTTACCAAAAGAAGCCTTGGGAGAGGAAGGACCAACAGGTCCAAAGGAAAAGGGTAATTGGAATGGTGATGAAGTTTGA
Protein sequence

>MS.gene000191.t1

MQASTFSKVWGAYEGGPGNRGASVYEPTGLTYGYSMLGSYSQPNSKPLFGYVLVAKDISGKTNGTLKPPVDYSLVLNTASATVTQDSPLYIWLPIAPHGYHAVGLVVTKTQDKPSPNKVMCVRSDLTEQCESSTLIWGSKGLNFYDVRPINRGTKAPGVRVGTFVAQNGGNSNPPSISCLKNIDSITQIMPSKKQIDALLQVNAPFLYLHSDEEYLPSSVNWFFSNGALLYKKGHESNPVPIAQNGNNLPQDPNTDGSYWLDLPSDNANKERVKKGNLQSAESYVHVKPMYGGTFTDIAMWVFYPFNGPGRAKVEFIDIKLGKAGEHVGDWEHVTLRVSNFDGQLWHIYFSQHNAGKWIDSSQLEFQSDASSFDFPTKRPIVYASLHGHASYPHAGINLLGKNGVGARDDTNKGRNVMDMGKYVLVSAEYLGSEVKEPAWLNYFREWGPHIDFDLDIELQNVAKALPGKLRDGFESIIRSLPKEALGEEGPTGPKEKGNWNGDEV