AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043859.t1 MTR_2g062430 95.841 553 23 0 1 553 1 553 0.0 1102
MS.gene043859.t1 MTR_2g062410 78.650 548 110 2 13 553 39 586 0.0 907
MS.gene043859.t1 MTR_2g062370 73.098 539 136 1 24 553 52 590 0.0 842
MS.gene043859.t1 MTR_4g131710 70.471 552 152 2 13 553 35 586 0.0 811
MS.gene043859.t1 MTR_2g062380 73.180 522 130 2 41 553 40 560 0.0 806
MS.gene043859.t1 MTR_2g067640 60.989 546 188 9 19 553 10 541 0.0 669
MS.gene043859.t1 MTR_2g062340 55.941 547 190 10 18 552 9 516 0.0 585
MS.gene043859.t1 MTR_1g080370 47.788 565 263 11 8 552 6 558 1.58e-176 510
MS.gene043859.t1 MTR_1g080410 47.788 565 263 11 8 552 6 558 1.58e-176 510
MS.gene043859.t1 MTR_5g007860 46.765 541 259 9 38 552 68 605 2.01e-170 496
MS.gene043859.t1 MTR_4g077620 46.410 571 283 13 1 552 1 567 1.34e-168 490
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043859.t1 AT2G44260 58.348 557 222 5 1 552 1 552 0.0 674
MS.gene043859.t1 AT2G44260 55.537 587 221 6 1 552 1 582 0.0 661
MS.gene043859.t1 AT2G44230 55.556 549 228 7 12 553 3 542 0.0 620
MS.gene043859.t1 AT3G01870 51.013 543 248 8 20 552 49 583 0.0 549
MS.gene043859.t1 AT3G01880 50.735 544 250 8 20 552 53 589 0.0 533
MS.gene043859.t1 AT5G18490 46.939 539 261 9 29 553 25 552 4.11e-171 496
MS.gene043859.t1 AT3G04350 46.606 545 267 9 29 553 26 566 1.86e-168 489
MS.gene043859.t1 AT3G04350 46.717 533 260 9 41 553 16 544 1.20e-165 482
MS.gene043859.t1 AT5G43950 44.649 542 281 9 29 552 24 564 1.07e-161 473
MS.gene043859.t1 AT1G04090 43.920 551 278 13 29 552 24 570 3.17e-159 466

Find 118 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTTCTGCTGATTATTT+AGG 0.124329 2.2:-27186093 MS.gene043859:CDS
GCTCTTCCTGGTCCATTAAA+AGG 0.142099 2.2:+27186426 None:intergenic
TGCTAAGAGTGAAATGATTT+TGG 0.186859 2.2:-27186133 MS.gene043859:CDS
TATCAACATTTAACAAAGTT+TGG 0.199497 2.2:-27187334 MS.gene043859:CDS
AAGTTCATAAATGTTAAATT+AGG 0.202582 2.2:-27186396 MS.gene043859:CDS
ACTTACCTTGCTCGGTTAAT+TGG 0.203183 2.2:-27186695 MS.gene043859:CDS
GCGAGTCTTCTTCGTGGATT+TGG 0.208690 2.2:-27186950 MS.gene043859:CDS
AAGATACCACATGTTATATT+TGG 0.212174 2.2:-27187085 MS.gene043859:CDS
CGAGTCTTCTTCGTGGATTT+GGG 0.218843 2.2:-27186949 MS.gene043859:CDS
GTTGAACACATCAACTATTA+AGG 0.248916 2.2:-27187114 MS.gene043859:CDS
TGATTTCTGCTGATTATTTA+GGG 0.258266 2.2:-27186092 MS.gene043859:CDS
GTCCAAAGCTGAAGAATAAT+TGG 0.267074 2.2:-27185888 MS.gene043859:CDS
TCCTCTCCCAGCACTTCTTC+TGG 0.294781 2.2:+27185922 None:intergenic
CTCCAATTATTCTTCAGCTT+TGG 0.329586 2.2:+27185886 None:intergenic
ATTGGGAGCATGTGACATTA+AGG 0.331662 2.2:-27186350 MS.gene043859:CDS
GGCTCATTCGTTGCACAAAA+TGG 0.331734 2.2:-27186855 MS.gene043859:CDS
GCAAATGGAATCATTGATCT+AGG 0.334564 2.2:-27187377 MS.gene043859:CDS
ATCAACATTTAACAAAGTTT+GGG 0.335558 2.2:-27187333 MS.gene043859:CDS
AATGTTAAATTAGGGAAAAT+AGG 0.338306 2.2:-27186387 MS.gene043859:CDS
GTAATTTAGATGGTCAATTA+TGG 0.338445 2.2:-27186323 MS.gene043859:CDS
GGCCAAGCATTTGGAGCAAT+TGG 0.358291 2.2:+27187951 None:intergenic
AGTTCATAAATGTTAAATTA+GGG 0.360247 2.2:-27186395 MS.gene043859:CDS
GCATCAATTTGTACTAGATT+AGG 0.374766 2.2:+27186768 None:intergenic
AGATGAGGAGTTGAGGAAAG+TGG 0.382577 2.2:-27186001 MS.gene043859:CDS
ATACCTGGTGGCCAAGCATT+TGG 0.390184 2.2:+27187942 None:intergenic
CCTAACATAGAGAATCCTTT+AGG 0.394355 2.2:+27187251 None:intergenic
ATTATCTGGTCCACCATCAT+AGG 0.395820 2.2:+27187307 None:intergenic
AGCAATTGGAAGCTTGAATA+TGG 0.401497 2.2:+27187965 None:intergenic
ATTGTGGCACCTTGATTATC+TGG 0.402581 2.2:+27187293 None:intergenic
AACACAAGTGAGTAATCAAT+TGG 0.404860 2.2:+27187134 None:intergenic
CCTAAAGCTTTGTATCCATT+AGG 0.407577 2.2:+27187050 None:intergenic
CCTAATGGATACAAAGCTTT+AGG 0.409137 2.2:-27187050 MS.gene043859:CDS
TTGTATCCATTAGGTGCTAT+TGG 0.410472 2.2:+27187059 None:intergenic
CCTAAAGGATTCTCTATGTT+AGG 0.416282 2.2:-27187251 MS.gene043859:CDS
CTAACAATGATGGTACCTAT+TGG 0.429733 2.2:-27186581 MS.gene043859:CDS
TTCTTCAGCTTTGGACCTGT+GGG 0.433050 2.2:+27185895 None:intergenic
CTTGTATAGTAATGCTCCAT+TGG 0.433338 2.2:+27186665 None:intergenic
CCTGTTCCAATAGCACAAAA+TGG 0.435052 2.2:-27186624 MS.gene043859:CDS
CAATAGGTACCATCATTGTT+AGG 0.437937 2.2:+27186582 None:intergenic
GCTTGAGTACCTCTATTGAT+TGG 0.438682 2.2:+27186894 None:intergenic
CAAGGTAAGTATTCTTCATC+AGG 0.438770 2.2:+27186708 None:intergenic
CTCTTCCTGGTCCATTAAAA+GGG 0.442838 2.2:+27186427 None:intergenic
TTCCAATTGCTCCAAATGCT+TGG 0.445747 2.2:-27187953 MS.gene043859:CDS
GAGTTTACCAGAAGAAGTGC+TGG 0.446564 2.2:-27185929 MS.gene043859:CDS
GCTCCAAATGCTTGGCCACC+AGG 0.451485 2.2:-27187945 MS.gene043859:intron
ATTCTTCAGCTTTGGACCTG+TGG 0.451562 2.2:+27185894 None:intergenic
CATGAAGGACTAGTTCTTCA+AGG 0.455081 2.2:-27186186 MS.gene043859:CDS
CACAACATAGTAAAGGTGAA+TGG 0.465087 2.2:-27186287 MS.gene043859:CDS
GTGAAGGTTCCTCCTAGCAT+TGG 0.467545 2.2:+27186474 None:intergenic
TGGCTACCAATAGCACCTAA+TGG 0.475290 2.2:-27187065 MS.gene043859:CDS
TAGGTGAACATGTTGGTGAT+TGG 0.478019 2.2:-27186368 MS.gene043859:CDS
AGAAGGTAGGTCAAGCCAAT+AGG 0.478817 2.2:+27186566 None:intergenic
AGCTTGAATATGGTGTCAAC+GGG 0.480366 2.2:+27187975 None:intergenic
AAAGTTTGGGCCACCTATGA+TGG 0.480692 2.2:-27187320 MS.gene043859:CDS
GCCACAATATTTGAACCAAC+AGG 0.497066 2.2:-27187278 MS.gene043859:CDS
ACAACATAGTAAAGGTGAAT+GGG 0.499232 2.2:-27186286 MS.gene043859:CDS
GAATATGGTGTCAACGGGAC+GGG 0.502650 2.2:+27187980 None:intergenic
GAAGAAGTGCTGGGAGAGGA+AGG 0.504490 2.2:-27185919 MS.gene043859:CDS
TCAGGATGTAAGTACATCAA+TGG 0.505882 2.2:+27186726 None:intergenic
TTAAGGGTGAGTAATTTAGA+TGG 0.508208 2.2:-27186333 MS.gene043859:CDS
ACCAATGCGAGTCTTCTTCG+TGG 0.508222 2.2:-27186956 MS.gene043859:CDS
AGGTGAACATGTTGGTGATT+GGG 0.511142 2.2:-27186367 MS.gene043859:CDS
CTGGGAGAGGAAGGACCCAC+AGG 0.511477 2.2:-27185910 MS.gene043859:CDS
CAAGGTGGCTCTATAACAAA+AGG 0.513235 2.2:+27186069 None:intergenic
ACCTGTTGGTTCAAATATTG+TGG 0.520433 2.2:+27187277 None:intergenic
GAACCAACAGGTATACCTAA+AGG 0.523818 2.2:-27187266 MS.gene043859:CDS
TGGATATGTTCTTGTGGCAA+AGG 0.533432 2.2:-27187195 MS.gene043859:CDS
CCTCAGGATCCTAACAATGA+TGG 0.535568 2.2:-27186591 MS.gene043859:CDS
AATAGAGGTACTCAAGCTGC+TGG 0.536143 2.2:-27186888 MS.gene043859:CDS
TGAAGAATACTTACCTTGCT+CGG 0.536982 2.2:-27186703 MS.gene043859:CDS
AAATGGTATTAACCTTCCTC+AGG 0.538181 2.2:-27186607 MS.gene043859:CDS
TGTTTGCATCATCAGAAGGT+AGG 0.539120 2.2:+27186553 None:intergenic
AAAGAGGTTTGTTGTTGGGT+TGG 0.542937 2.2:+27187219 None:intergenic
ACCAGAAGAAGTGCTGGGAG+AGG 0.544118 2.2:-27185923 MS.gene043859:CDS
TCATTCGTTGCACAAAATGG+TGG 0.544600 2.2:-27186852 MS.gene043859:CDS
TTCAAACATGAAATAGATGG+AGG 0.553594 2.2:+27186819 None:intergenic
CTTGTGTTGTGGTGACAACA+TGG 0.561956 2.2:+27187027 None:intergenic
TCTTTGTTTGCATCATCAGA+AGG 0.565098 2.2:+27186549 None:intergenic
TCCACGAAGAAGACTCGCAT+TGG 0.565993 2.2:+27186955 None:intergenic
GGAGCATTACTATACAAGAA+AGG 0.567167 2.2:-27186660 MS.gene043859:CDS
CATTGTTAGGATCCTGAGGA+AGG 0.572703 2.2:+27186595 None:intergenic
CAAGCTGCTGGTGTTCGTGT+CGG 0.577708 2.2:-27186876 MS.gene043859:CDS
ATAATATAGATGAGGAGTTG+AGG 0.582445 2.2:-27186008 MS.gene043859:CDS
CCACATGGCAATATCTGTGA+AGG 0.582501 2.2:+27186458 None:intergenic
AAGCTTGAATATGGTGTCAA+CGG 0.583843 2.2:+27187974 None:intergenic
CTGATGATGCAAACAAAGAG+AGG 0.585320 2.2:-27186545 MS.gene043859:CDS
GATGGTGGACCAGATAATCA+AGG 0.585776 2.2:-27187302 MS.gene043859:CDS
TATTTCTCACAACATAGTAA+AGG 0.585924 2.2:-27186294 MS.gene043859:CDS
AATCCTTTAGGTATACCTGT+TGG 0.587379 2.2:+27187263 None:intergenic
TGAATATGGTGTCAACGGGA+CGG 0.587459 2.2:+27187979 None:intergenic
GAGTCTTCTTCGTGGATTTG+GGG 0.588057 2.2:-27186948 MS.gene043859:CDS
ATGAAGGACTAGTTCTTCAA+GGG 0.592808 2.2:-27186185 MS.gene043859:CDS
TCAAACATGAAATAGATGGA+GGG 0.592826 2.2:+27186820 None:intergenic
CATTCGTTGCACAAAATGGT+GGG 0.596880 2.2:-27186851 MS.gene043859:CDS
GGGAAAATAGGTGAACATGT+TGG 0.598707 2.2:-27186375 MS.gene043859:CDS
GATGTTAGACCAATCAATAG+AGG 0.600235 2.2:-27186903 MS.gene043859:CDS
ATCTGGTCCACCATCATAGG+TGG 0.604053 2.2:+27187310 None:intergenic
TTCCTAAAATAGTGCAACCA+AGG 0.605969 2.2:+27186051 None:intergenic
CATGCATCATATCCACATGA+AGG 0.613328 2.2:-27186201 MS.gene043859:CDS
CCATCATTGTTAGGATCCTG+AGG 0.614872 2.2:+27186591 None:intergenic
GTACATGTGAAACCAATGCT+AGG 0.617083 2.2:-27186486 MS.gene043859:CDS
TTGGGAGCATGTGACATTAA+GGG 0.618313 2.2:-27186349 MS.gene043859:CDS
TGATGATGCAAACAAAGAGA+GGG 0.621716 2.2:-27186544 MS.gene043859:CDS
AGTTTACCAGAAGAAGTGCT+GGG 0.622675 2.2:-27185928 MS.gene043859:CDS
GGAAGGTTTATCTTGTGTTG+TGG 0.622916 2.2:+27187016 None:intergenic
AAGCTGAAGAATAATTGGAG+TGG 0.625543 2.2:-27185883 MS.gene043859:CDS
GTTTGGGCCACCTATGATGG+TGG 0.627591 2.2:-27187317 MS.gene043859:CDS
AAGTGGAGAAGATATTGCCA+TGG 0.635901 2.2:-27185984 MS.gene043859:CDS
AATGGAATCATTGATCTAGG+TGG 0.649257 2.2:-27187374 MS.gene043859:CDS
TGGTAGCCAAATATAACATG+TGG 0.651391 2.2:+27187079 None:intergenic
CTAAAATAGTGCAACCAAGG+TGG 0.652677 2.2:+27186054 None:intergenic
AATTGATTATAATATAGATG+AGG 0.654441 2.2:-27186016 MS.gene043859:CDS
TGAAGAACTAGTCCTTCATG+TGG 0.664200 2.2:+27186189 None:intergenic
AAGACTCGCATTGGTCTGTG+AGG 0.675872 2.2:+27186964 None:intergenic
CTTCATGTGGATATGATGCA+TGG 0.678841 2.2:+27186202 None:intergenic
CATGTGAAACCAATGCTAGG+AGG 0.684488 2.2:-27186483 MS.gene043859:CDS
AAAAGGGTAAAATATCCACA+TGG 0.728172 2.2:+27186443 None:intergenic
CCTTCACAGATATTGCCATG+TGG 0.751886 2.2:-27186458 MS.gene043859:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAAAATTATTTATA+AGG + chr2.2:27186120-27186139 None:intergenic 0.0%
!!! TCTATATTATAATCAATTTT+TGG + chr2.2:27187896-27187915 None:intergenic 10.0%
!! AAGTTCATAAATGTTAAATT+AGG - chr2.2:27187518-27187537 MS.gene043859:intron 15.0%
!! AATTGATTATAATATAGATG+AGG - chr2.2:27187898-27187917 MS.gene043859:intron 15.0%
!! AGTTCATAAATGTTAAATTA+GGG - chr2.2:27187519-27187538 MS.gene043859:intron 15.0%
!!! ACATTTTTGTTTATAATTCT+TGG + chr2.2:27186370-27186389 None:intergenic 15.0%
!!! TTTTACTTAACTAAAAATCA+AGG - chr2.2:27186318-27186337 MS.gene043859:CDS 15.0%
!! AATGTTAAATTAGGGAAAAT+AGG - chr2.2:27187527-27187546 MS.gene043859:intron 20.0%
!! AGCAAAAAGTGATATATTTA+TGG - chr2.2:27186423-27186442 MS.gene043859:CDS 20.0%
!! ATCAACATTTAACAAAGTTT+GGG - chr2.2:27186581-27186600 MS.gene043859:CDS 20.0%
!! CTTATGTAGTCAATATTTAA+GGG + chr2.2:27186240-27186259 None:intergenic 20.0%
!! TATCAACATTTAACAAAGTT+TGG - chr2.2:27186580-27186599 MS.gene043859:CDS 20.0%
!!! AAAATATCTTTTAGTTTCCA+TGG + chr2.2:27187950-27187969 None:intergenic 20.0%
!!! TTTTTCAAACATGAAATAGA+TGG + chr2.2:27187101-27187120 None:intergenic 20.0%
! AAGATACCACATGTTATATT+TGG - chr2.2:27186829-27186848 MS.gene043859:CDS 25.0%
! CCTTATGTAGTCAATATTTA+AGG + chr2.2:27186241-27186260 None:intergenic 25.0%
! CTCAAACTAAAAGTAAAAAG+TGG + chr2.2:27186073-27186092 None:intergenic 25.0%
! GCATTAACTAGATATATAAC+AGG + chr2.2:27186469-27186488 None:intergenic 25.0%
! GTAAAAAGTGGCATATTTAA+AGG + chr2.2:27186061-27186080 None:intergenic 25.0%
! GTAATTTAGATGGTCAATTA+TGG - chr2.2:27187591-27187610 MS.gene043859:intron 25.0%
! TATTTCTCACAACATAGTAA+AGG - chr2.2:27187620-27187639 MS.gene043859:intron 25.0%
! TGCAATGAAGAATAAAAAAC+TGG + chr2.2:27187689-27187708 None:intergenic 25.0%
!! CCTTAAATATTGACTACATA+AGG - chr2.2:27186238-27186257 MS.gene043859:CDS 25.0%
!! TGATTTCTGCTGATTATTTA+GGG - chr2.2:27187822-27187841 MS.gene043859:intron 25.0%
!! TGGATATTTTACCCTTTTAA+TGG - chr2.2:27187476-27187495 MS.gene043859:intron 25.0%
!! TTAATACCATTTTGTGCTAT+TGG + chr2.2:27187299-27187318 None:intergenic 25.0%
!! TTGATTTCTGCTGATTATTT+AGG - chr2.2:27187821-27187840 MS.gene043859:intron 25.0%
!!! GTTTTTTATTCTTCATTGCA+TGG - chr2.2:27187689-27187708 MS.gene043859:intron 25.0%
AAAAGGGTAAAATATCCACA+TGG + chr2.2:27187474-27187493 None:intergenic 30.0%
AACAAAGAGAGGGTAAAAAA+AGG - chr2.2:27187380-27187399 MS.gene043859:CDS 30.0%
AACACAAGTGAGTAATCAAT+TGG + chr2.2:27186783-27186802 None:intergenic 30.0%
ACAACATAGTAAAGGTGAAT+GGG - chr2.2:27187628-27187647 MS.gene043859:intron 30.0%
ACAAGAACATATCCAAAAAG+AGG + chr2.2:27186714-27186733 None:intergenic 30.0%
ATAATATAGATGAGGAGTTG+AGG - chr2.2:27187906-27187925 MS.gene043859:intron 30.0%
GCATCAATTTGTACTAGATT+AGG + chr2.2:27187149-27187168 None:intergenic 30.0%
GTTGAACACATCAACTATTA+AGG - chr2.2:27186800-27186819 MS.gene043859:CDS 30.0%
TCAAACATGAAATAGATGGA+GGG + chr2.2:27187097-27187116 None:intergenic 30.0%
TTAAGGGTGAGTAATTTAGA+TGG - chr2.2:27187581-27187600 MS.gene043859:intron 30.0%
TTCAAACATGAAATAGATGG+AGG + chr2.2:27187098-27187117 None:intergenic 30.0%
! AGTGTTGGATTGTTACATAT+AGG - chr2.2:27186504-27186523 MS.gene043859:CDS 30.0%
! TCTTTTTGGATATGTTCTTG+TGG - chr2.2:27186713-27186732 MS.gene043859:CDS 30.0%
! TGCTAAGAGTGAAATGATTT+TGG - chr2.2:27187781-27187800 MS.gene043859:intron 30.0%
!! GTTGCACTATTTTAGGAAAT+GGG - chr2.2:27187868-27187887 MS.gene043859:intron 30.0%
!! TTGCACTATTTTAGGAAATG+GGG - chr2.2:27187869-27187888 MS.gene043859:intron 30.0%
!!! GTTAATTGGTTTTTCTCCAA+TGG - chr2.2:27187233-27187252 MS.gene043859:CDS 30.0%
AAAAACCAATTAACCGAGCA+AGG + chr2.2:27187227-27187246 None:intergenic 35.0%
AAATGGTATTAACCTTCCTC+AGG - chr2.2:27187307-27187326 MS.gene043859:CDS 35.0%
AAGCTGAAGAATAATTGGAG+TGG - chr2.2:27188031-27188050 MS.gene043859:CDS 35.0%
AATCCTTTAGGTATACCTGT+TGG + chr2.2:27186654-27186673 None:intergenic 35.0%
AATGGAATCATTGATCTAGG+TGG - chr2.2:27186540-27186559 MS.gene043859:CDS 35.0%
ACAATCACACAATGAATACC+TGG + chr2.2:27185990-27186009 None:intergenic 35.0%
AGCAATTGGAAGCTTGAATA+TGG + chr2.2:27185952-27185971 None:intergenic 35.0%
CAAGGTAAGTATTCTTCATC+AGG + chr2.2:27187209-27187228 None:intergenic 35.0%
CAATAGGTACCATCATTGTT+AGG + chr2.2:27187335-27187354 None:intergenic 35.0%
CACAACATAGTAAAGGTGAA+TGG - chr2.2:27187627-27187646 MS.gene043859:intron 35.0%
CCTAAAGGATTCTCTATGTT+AGG - chr2.2:27186663-27186682 MS.gene043859:CDS 35.0%
CCTAACATAGAGAATCCTTT+AGG + chr2.2:27186666-27186685 None:intergenic 35.0%
CTAACAATGATGGTACCTAT+TGG - chr2.2:27187333-27187352 MS.gene043859:CDS 35.0%
CTCCAATTATTCTTCAGCTT+TGG + chr2.2:27188031-27188050 None:intergenic 35.0%
CTTCAAGGGAAAAATGGAAT+AGG - chr2.2:27187743-27187762 MS.gene043859:intron 35.0%
CTTGTATAGTAATGCTCCAT+TGG + chr2.2:27187252-27187271 None:intergenic 35.0%
GATGTTAGACCAATCAATAG+AGG - chr2.2:27187011-27187030 MS.gene043859:CDS 35.0%
GCAAATGGAATCATTGATCT+AGG - chr2.2:27186537-27186556 MS.gene043859:CDS 35.0%
GGATTGTTACATATAGGTAG+TGG - chr2.2:27186510-27186529 MS.gene043859:CDS 35.0%
GTCCAAAGCTGAAGAATAAT+TGG - chr2.2:27188026-27188045 MS.gene043859:CDS 35.0%
TCAGGATGTAAGTACATCAA+TGG + chr2.2:27187191-27187210 None:intergenic 35.0%
TGAAGAATACTTACCTTGCT+CGG - chr2.2:27187211-27187230 MS.gene043859:CDS 35.0%
TGGTAGCCAAATATAACATG+TGG + chr2.2:27186838-27186857 None:intergenic 35.0%
TTCCTAAAATAGTGCAACCA+AGG + chr2.2:27187866-27187885 None:intergenic 35.0%
! AAGCTTGAATATGGTGTCAA+CGG + chr2.2:27185943-27185962 None:intergenic 35.0%
! ATGCTGATTATAGCTAGTGT+TGG - chr2.2:27186489-27186508 MS.gene043859:CDS 35.0%
! CCTAAAGCTTTGTATCCATT+AGG + chr2.2:27186867-27186886 None:intergenic 35.0%
! CCTAATGGATACAAAGCTTT+AGG - chr2.2:27186864-27186883 MS.gene043859:CDS 35.0%
! CTAGTTCTTCAAGGGAAAAA+TGG - chr2.2:27187737-27187756 MS.gene043859:intron 35.0%
! GACACACATGATTTTGTCAA+GGG + chr2.2:27186922-27186941 None:intergenic 35.0%
! GGAGCATTACTATACAAGAA+AGG - chr2.2:27187254-27187273 MS.gene043859:CDS 35.0%
! TCTTTGTTTGCATCATCAGA+AGG + chr2.2:27187368-27187387 None:intergenic 35.0%
! TGACACACATGATTTTGTCA+AGG + chr2.2:27186923-27186942 None:intergenic 35.0%
! TGATGATGCAAACAAAGAGA+GGG - chr2.2:27187370-27187389 MS.gene043859:CDS 35.0%
! TTGTATCCATTAGGTGCTAT+TGG + chr2.2:27186858-27186877 None:intergenic 35.0%
!! AACTTTACTTTTGCTCTTCC+TGG + chr2.2:27187503-27187522 None:intergenic 35.0%
!! ACCTGTTGGTTCAAATATTG+TGG + chr2.2:27186640-27186659 None:intergenic 35.0%
!! ATAGGTAGTGGTTTTGCAAA+TGG - chr2.2:27186522-27186541 MS.gene043859:CDS 35.0%
!! ATGAAGGACTAGTTCTTCAA+GGG - chr2.2:27187729-27187748 MS.gene043859:intron 35.0%
!! CCAAAAAGAGGTTTGTTGTT+GGG + chr2.2:27186702-27186721 None:intergenic 35.0%
!! GGTTGCACTATTTTAGGAAA+TGG - chr2.2:27187867-27187886 MS.gene043859:intron 35.0%
!! TCCAAAAAGAGGTTTGTTGT+TGG + chr2.2:27186703-27186722 None:intergenic 35.0%
!! TTTTACCCTTTTAATGGACC+AGG - chr2.2:27187482-27187501 MS.gene043859:intron 35.0%
AAGTGGAGAAGATATTGCCA+TGG - chr2.2:27187930-27187949 MS.gene043859:intron 40.0%
ACTTACCTTGCTCGGTTAAT+TGG - chr2.2:27187219-27187238 MS.gene043859:CDS 40.0%
ATCACACAATGAATACCTGG+TGG + chr2.2:27185987-27186006 None:intergenic 40.0%
ATTATCTGGTCCACCATCAT+AGG + chr2.2:27186610-27186629 None:intergenic 40.0%
ATTGTGGCACCTTGATTATC+TGG + chr2.2:27186624-27186643 None:intergenic 40.0%
CATGCATCATATCCACATGA+AGG - chr2.2:27187713-27187732 MS.gene043859:intron 40.0%
CATTCGTTGCACAAAATGGT+GGG - chr2.2:27187063-27187082 MS.gene043859:CDS 40.0%
CCTGTTCCAATAGCACAAAA+TGG - chr2.2:27187290-27187309 MS.gene043859:CDS 40.0%
CTAAAATAGTGCAACCAAGG+TGG + chr2.2:27187863-27187882 None:intergenic 40.0%
CTCTTCCTGGTCCATTAAAA+GGG + chr2.2:27187490-27187509 None:intergenic 40.0%
CTTCATGTGGATATGATGCA+TGG + chr2.2:27187715-27187734 None:intergenic 40.0%
GAACCAACAGGTATACCTAA+AGG - chr2.2:27186648-27186667 MS.gene043859:CDS 40.0%
GCCACAATATTTGAACCAAC+AGG - chr2.2:27186636-27186655 MS.gene043859:CDS 40.0%
GCTTGAGTACCTCTATTGAT+TGG + chr2.2:27187023-27187042 None:intergenic 40.0%
GGAAGGTTTATCTTGTGTTG+TGG + chr2.2:27186901-27186920 None:intergenic 40.0%
GGGAAAATAGGTGAACATGT+TGG - chr2.2:27187539-27187558 MS.gene043859:intron 40.0%
GTACATGTGAAACCAATGCT+AGG - chr2.2:27187428-27187447 MS.gene043859:intron 40.0%
TCATTCGTTGCACAAAATGG+TGG - chr2.2:27187062-27187081 MS.gene043859:CDS 40.0%
TGAAGAACTAGTCCTTCATG+TGG + chr2.2:27187728-27187747 None:intergenic 40.0%
TGGATATGTTCTTGTGGCAA+AGG - chr2.2:27186719-27186738 MS.gene043859:CDS 40.0%
TGTTTGCATCATCAGAAGGT+AGG + chr2.2:27187364-27187383 None:intergenic 40.0%
TTCCAATTGCTCCAAATGCT+TGG - chr2.2:27185961-27185980 MS.gene043859:CDS 40.0%
! AGCTTGAATATGGTGTCAAC+GGG + chr2.2:27185942-27185961 None:intergenic 40.0%
! ATTGGGAGCATGTGACATTA+AGG - chr2.2:27187564-27187583 MS.gene043859:intron 40.0%
! CAAGGTGGCTCTATAACAAA+AGG + chr2.2:27187848-27187867 None:intergenic 40.0%
! CACATGATTTTGTCAAGGGA+AGG + chr2.2:27186918-27186937 None:intergenic 40.0%
! CACCTTGGTTGCACTATTTT+AGG - chr2.2:27187861-27187880 MS.gene043859:intron 40.0%
! CCCAACAACAAACCTCTTTT+TGG - chr2.2:27186699-27186718 MS.gene043859:CDS 40.0%
! CTGATGATGCAAACAAAGAG+AGG - chr2.2:27187369-27187388 MS.gene043859:CDS 40.0%
! TTGGGAGCATGTGACATTAA+GGG - chr2.2:27187565-27187584 MS.gene043859:intron 40.0%
!! AAAGAGGTTTGTTGTTGGGT+TGG + chr2.2:27186698-27186717 None:intergenic 40.0%
!! AGGTGAACATGTTGGTGATT+GGG - chr2.2:27187547-27187566 MS.gene043859:intron 40.0%
!! AGTTTACCAGAAGAAGTGCT+GGG - chr2.2:27187986-27188005 MS.gene043859:CDS 40.0%
!! CATGAAGGACTAGTTCTTCA+AGG - chr2.2:27187728-27187747 MS.gene043859:intron 40.0%
!! CCATTTTGTGCTATTGGAAC+AGG + chr2.2:27187293-27187312 None:intergenic 40.0%
!! CTTTTGTTATAGAGCCACCT+TGG - chr2.2:27187846-27187865 MS.gene043859:intron 40.0%
!! TAGGTGAACATGTTGGTGAT+TGG - chr2.2:27187546-27187565 MS.gene043859:intron 40.0%
AAAGTTTGGGCCACCTATGA+TGG - chr2.2:27186594-27186613 MS.gene043859:CDS 45.0%
AGAAGGTAGGTCAAGCCAAT+AGG + chr2.2:27187351-27187370 None:intergenic 45.0%
AGATGAGGAGTTGAGGAAAG+TGG - chr2.2:27187913-27187932 MS.gene043859:intron 45.0%
CATGTGAAACCAATGCTAGG+AGG - chr2.2:27187431-27187450 MS.gene043859:intron 45.0%
CATTGTTAGGATCCTGAGGA+AGG + chr2.2:27187322-27187341 None:intergenic 45.0%
CCACATGGCAATATCTGTGA+AGG + chr2.2:27187459-27187478 None:intergenic 45.0%
CCATCATTGTTAGGATCCTG+AGG + chr2.2:27187326-27187345 None:intergenic 45.0%
CCTCAGGATCCTAACAATGA+TGG - chr2.2:27187323-27187342 MS.gene043859:CDS 45.0%
CCTTCACAGATATTGCCATG+TGG - chr2.2:27187456-27187475 MS.gene043859:intron 45.0%
CGAGTCTTCTTCGTGGATTT+GGG - chr2.2:27186965-27186984 MS.gene043859:CDS 45.0%
CTTGTGTTGTGGTGACAACA+TGG + chr2.2:27186890-27186909 None:intergenic 45.0%
GAGTCTTCTTCGTGGATTTG+GGG - chr2.2:27186966-27186985 MS.gene043859:CDS 45.0%
GCTCTTCCTGGTCCATTAAA+AGG + chr2.2:27187491-27187510 None:intergenic 45.0%
GGCTCATTCGTTGCACAAAA+TGG - chr2.2:27187059-27187078 MS.gene043859:CDS 45.0%
TGGCTACCAATAGCACCTAA+TGG - chr2.2:27186849-27186868 MS.gene043859:CDS 45.0%
! GAGTTTACCAGAAGAAGTGC+TGG - chr2.2:27187985-27188004 MS.gene043859:CDS 45.0%
! TGAATATGGTGTCAACGGGA+CGG + chr2.2:27185938-27185957 None:intergenic 45.0%
!! AATAGAGGTACTCAAGCTGC+TGG - chr2.2:27187026-27187045 MS.gene043859:CDS 45.0%
!! ATTCTTCAGCTTTGGACCTG+TGG + chr2.2:27188023-27188042 None:intergenic 45.0%
!! GATGGTGGACCAGATAATCA+AGG - chr2.2:27186612-27186631 MS.gene043859:CDS 45.0%
!! TTCTTCAGCTTTGGACCTGT+GGG + chr2.2:27188022-27188041 None:intergenic 45.0%
!!! ATATTTTTGAAAATATTATT+AGG - chr2.2:27187963-27187982 MS.gene043859:CDS 5.0%
ACCAATGCGAGTCTTCTTCG+TGG - chr2.2:27186958-27186977 MS.gene043859:CDS 50.0%
ATACCTGGTGGCCAAGCATT+TGG + chr2.2:27185975-27185994 None:intergenic 50.0%
ATCTGGTCCACCATCATAGG+TGG + chr2.2:27186607-27186626 None:intergenic 50.0%
GCGAGTCTTCTTCGTGGATT+TGG - chr2.2:27186964-27186983 MS.gene043859:CDS 50.0%
GTGAAGGTTCCTCCTAGCAT+TGG + chr2.2:27187443-27187462 None:intergenic 50.0%
TCCACGAAGAAGACTCGCAT+TGG + chr2.2:27186962-27186981 None:intergenic 50.0%
! AAGACTCGCATTGGTCTGTG+AGG + chr2.2:27186953-27186972 None:intergenic 50.0%
! GAATATGGTGTCAACGGGAC+GGG + chr2.2:27185937-27185956 None:intergenic 50.0%
! GGCCAAGCATTTGGAGCAAT+TGG + chr2.2:27185966-27185985 None:intergenic 50.0%
CAAGCTGCTGGTGTTCGTGT+CGG - chr2.2:27187038-27187057 MS.gene043859:CDS 55.0%
GTTTGGGCCACCTATGATGG+TGG - chr2.2:27186597-27186616 MS.gene043859:CDS 55.0%
TCCTCTCCCAGCACTTCTTC+TGG + chr2.2:27187995-27188014 None:intergenic 55.0%
!! ACCAGAAGAAGTGCTGGGAG+AGG - chr2.2:27187991-27188010 MS.gene043859:CDS 55.0%
!! GAAGAAGTGCTGGGAGAGGA+AGG - chr2.2:27187995-27188014 MS.gene043859:CDS 55.0%
GCTCCAAATGCTTGGCCACC+AGG - chr2.2:27185969-27185988 MS.gene043859:CDS 60.0%
CTGGGAGAGGAAGGACCCAC+AGG - chr2.2:27188004-27188023 MS.gene043859:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 27185870 27188066 27185870 ID=MS.gene043859
chr2.2 mRNA 27185870 27188066 27185870 ID=MS.gene043859.t1;Parent=MS.gene043859
chr2.2 exon 27187946 27188066 27187946 ID=MS.gene043859.t1.exon1;Parent=MS.gene043859.t1
chr2.2 CDS 27187946 27188066 27187946 ID=cds.MS.gene043859.t1;Parent=MS.gene043859.t1
chr2.2 exon 27185870 27187410 27185870 ID=MS.gene043859.t1.exon2;Parent=MS.gene043859.t1
chr2.2 CDS 27185870 27187410 27185870 ID=cds.MS.gene043859.t1;Parent=MS.gene043859.t1
Gene Sequence

>MS.gene043859

ATGGATAATTGTCTTTCTTCATGTTGTTGTGTAGATAATCTCTTCAAGAGAAAGCAATCTCGATCCCGTCCCGTTGACACCATATTCAAGCTTCCAATTGCTCCAAATGCTTGGCCACCAGGTATTCATTGTGTGATTGTCAATTTCAGTCTTCTTGTGATATTATATAAGTTTTATATGATTTTTTTCCTTTAAATATGCCACTTTTTACTTTTAGTTTGAGTAAAAAATATTTCAAGATTTTATTCCTTATAAATAATTTTTATTTTATTTTTTTGCTTTTGATCTTTGTAGTTTCTTTTTAGTTGCTGAAATATTTTTTACGTTAGAATTGATCATTTTAATATGTAAAATCTTTGATTTTAGTCCCTTAAATATTGACTACATAAGGACTAATTAAAATGAATGTTACACTAATATTACATTGATTAATTGAAGCATGAAGAAATTTTACTTAACTAAAAATCAAGGATCAAAAGTAAAAAAGTTGTATGTTTCCAAGAATTATAAACAAAAATGTTTTTACATAAACTATGTTTTTACATAAACTAAAAGCAAAAAGTGATATATTTATGGTTATCAAAAATATATTTAAACCTGTTATATATCTAGTTAATGCATGCTGATTATAGCTAGTGTTGGATTGTTACATATAGGTAGTGGTTTTGCAAATGGAATCATTGATCTAGGTGGACTTCAAGTGTCACAAGTATCAACATTTAACAAAGTTTGGGCCACCTATGATGGTGGACCAGATAATCAAGGTGCCACAATATTTGAACCAACAGGTATACCTAAAGGATTCTCTATGTTAGGTAGCTACAGCCAACCCAACAACAAACCTCTTTTTGGATATGTTCTTGTGGCAAAGGATGTTTCTTCAAGTACTACAAATAGCACTTTAAAGAAACCAATTGATTACTCACTTGTGTTGAACACATCAACTATTAAGGTTAATCAAGATACCACATGTTATATTTGGCTACCAATAGCACCTAATGGATACAAAGCTTTAGGCCATGTTGTCACCACAACACAAGATAAACCTTCCCTTGACAAAATCATGTGTGTCAGATCAGACCTCACAGACCAATGCGAGTCTTCTTCGTGGATTTGGGGATCAAACGACTTCAATTTTTATGATGTTAGACCAATCAATAGAGGTACTCAAGCTGCTGGTGTTCGTGTCGGCTCATTCGTTGCACAAAATGGTGGGACTAATGTCCCTCCATCTATTTCATGTTTGAAAAATCTCAATTCAATTTCAAAAATCATGCCTAATCTAGTACAAATTGATGCAATACTCAAAGTTTACTCTCCATTGATGTACTTACATCCTGATGAAGAATACTTACCTTGCTCGGTTAATTGGTTTTTCTCCAATGGAGCATTACTATACAAGAAAGGTGAAGAGTCAAATCCTGTTCCAATAGCACAAAATGGTATTAACCTTCCTCAGGATCCTAACAATGATGGTACCTATTGGCTTGACCTACCTTCTGATGATGCAAACAAAGAGAGGGTAAAAAAAGGAAATTTACAAAGTGCAGAATCTTATGTACATGTGAAACCAATGCTAGGAGGAACCTTCACAGATATTGCCATGTGGATATTTTACCCTTTTAATGGACCAGGAAGAGCAAAAGTAAAGTTCATAAATGTTAAATTAGGGAAAATAGGTGAACATGTTGGTGATTGGGAGCATGTGACATTAAGGGTGAGTAATTTAGATGGTCAATTATGGCATGTATATTTCTCACAACATAGTAAAGGTGAATGGGTTGATTCTTCTCAGCTTGAGTTTCAAACAAATAGACCAGTTTTTTATTCTTCATTGCATGGCCATGCATCATATCCACATGAAGGACTAGTTCTTCAAGGGAAAAATGGAATAGGTGTTAGAAATGATAGTGCTAAGAGTGAAATGATTTTGGATTTGACAAAATTTGTATTGATTTCTGCTGATTATTTAGGGCCTTTTGTTATAGAGCCACCTTGGTTGCACTATTTTAGGAAATGGGGTCCAAAAATTGATTATAATATAGATGAGGAGTTGAGGAAAGTGGAGAAGATATTGCCATGGAAACTAAAAGATATTTTTGAAAATATTATTAGGAGTTTACCAGAAGAAGTGCTGGGAGAGGAAGGACCCACAGGTCCAAAGCTGAAGAATAATTGGAGTGGAGATGAAGTTTGA
Protein sequence

>MS.gene043859.t1

MDNCLSSCCCVDNLFKRKQSRSRPVDTIFKLPIAPNAWPPGSGFANGIIDLGGLQVSQVSTFNKVWATYDGGPDNQGATIFEPTGIPKGFSMLGSYSQPNNKPLFGYVLVAKDVSSSTTNSTLKKPIDYSLVLNTSTIKVNQDTTCYIWLPIAPNGYKALGHVVTTTQDKPSLDKIMCVRSDLTDQCESSSWIWGSNDFNFYDVRPINRGTQAAGVRVGSFVAQNGGTNVPPSISCLKNLNSISKIMPNLVQIDAILKVYSPLMYLHPDEEYLPCSVNWFFSNGALLYKKGEESNPVPIAQNGINLPQDPNNDGTYWLDLPSDDANKERVKKGNLQSAESYVHVKPMLGGTFTDIAMWIFYPFNGPGRAKVKFINVKLGKIGEHVGDWEHVTLRVSNLDGQLWHVYFSQHSKGEWVDSSQLEFQTNRPVFYSSLHGHASYPHEGLVLQGKNGIGVRNDSAKSEMILDLTKFVLISADYLGPFVIEPPWLHYFRKWGPKIDYNIDEELRKVEKILPWKLKDIFENIIRSLPEEVLGEEGPTGPKLKNNWSGDEV