AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60887

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60887.t1 MTR_2g062380 93.750 560 35 0 1 560 1 560 0.0 1094
MS.gene60887.t1 MTR_2g062370 82.584 534 91 2 28 560 58 590 0.0 924
MS.gene60887.t1 MTR_2g062410 80.337 534 99 3 28 560 58 586 0.0 891
MS.gene60887.t1 MTR_2g062430 72.031 522 136 2 40 560 41 553 0.0 800
MS.gene60887.t1 MTR_4g131710 70.476 525 152 2 39 560 62 586 0.0 780
MS.gene60887.t1 MTR_2g067640 53.989 539 217 8 32 560 24 541 0.0 591
MS.gene60887.t1 MTR_2g062340 50.847 531 204 10 40 559 32 516 2.60e-178 513
MS.gene60887.t1 MTR_1g080370 45.353 538 264 9 39 559 34 558 3.53e-158 463
MS.gene60887.t1 MTR_1g080410 45.353 538 264 9 39 559 34 558 3.53e-158 463
MS.gene60887.t1 MTR_5g007860 42.451 563 290 10 22 559 52 605 1.73e-153 453
MS.gene60887.t1 MTR_4g077620 43.911 542 270 13 39 559 39 567 1.05e-145 432
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60887.t1 AT2G44260 54.802 531 228 5 31 559 62 582 0.0 601
MS.gene60887.t1 AT2G44260 55.067 523 223 5 39 559 40 552 0.0 598
MS.gene60887.t1 AT2G44230 52.762 525 230 7 40 560 32 542 0.0 558
MS.gene60887.t1 AT3G01870 47.917 528 252 7 40 559 71 583 1.29e-174 506
MS.gene60887.t1 AT3G01880 47.348 528 257 7 40 559 75 589 7.88e-170 494
MS.gene60887.t1 AT5G18490 45.047 535 263 9 39 560 36 552 1.91e-158 464
MS.gene60887.t1 AT3G04350 43.623 541 273 9 40 560 16 544 3.45e-150 442
MS.gene60887.t1 AT3G04350 43.542 542 274 9 39 560 37 566 6.06e-150 442
MS.gene60887.t1 AT5G43950 42.647 544 289 9 32 559 28 564 2.25e-146 433
MS.gene60887.t1 AT1G04090 42.857 546 275 12 40 559 36 570 2.63e-146 434

Find 136 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGACTCTCCTTTGTATATT+TGG 0.127143 2.3:+32006360 MS.gene60887:CDS
CAGCATGGTTGAATTATTTC+AGG 0.166486 2.3:+32007419 MS.gene60887:CDS
GCTTTAATTCCTCTATTTAT+TGG 0.227005 2.3:-32006551 None:intergenic
TTGGTTTCTGCAGAATATTT+AGG 0.246737 2.3:+32007379 MS.gene60887:CDS
TTCCTGTTTCACAACCTTCT+TGG 0.254181 2.3:+32005937 MS.gene60887:CDS
CTTGTAGCAAAGGATATTTC+AGG 0.255851 2.3:+32006263 MS.gene60887:CDS
TCCTCTCCCAAGGCTTCTTT+TGG 0.261151 2.3:-32007550 None:intergenic
GAGTTTATAGATATTAAATT+AGG 0.262170 2.3:+32007049 MS.gene60887:CDS
CTCAACACAATGCTGGTAAA+TGG 0.273775 2.3:+32007158 MS.gene60887:CDS
CATCAACATTCAGCAAAGTT+TGG 0.275579 2.3:+32006114 MS.gene60887:CDS
AGGTAGAAATGTTATGGATA+TGG 0.277907 2.3:+32007345 MS.gene60887:CDS
GTCCAAAGGAAAAGGGTAAT+TGG 0.292083 2.3:+32007584 MS.gene60887:CDS
TGGTTTCTGCAGAATATTTA+GGG 0.297210 2.3:+32007380 MS.gene60887:CDS
TGAATCGTCTACAGTGATTT+GGG 0.308695 2.3:+32006496 MS.gene60887:CDS
AACTCTACTTTAGCCCTTCC+AGG 0.316265 2.3:-32007031 None:intergenic
ATCAACATTCAGCAAAGTTT+GGG 0.318101 2.3:+32006115 MS.gene60887:CDS
GATGACTGTGTTTGCAAAAC+TGG 0.332977 2.3:-32006063 None:intergenic
GTGAATCGTCTACAGTGATT+TGG 0.337331 2.3:+32006495 MS.gene60887:CDS
GTAACTTCGATGGTCAATTA+TGG 0.344009 2.3:+32007122 MS.gene60887:CDS
GGAAGGTTTATCTTGTGTTT+TGG 0.350645 2.3:-32006429 None:intergenic
AACACACATGACTTTGTTGA+TGG 0.351094 2.3:-32006450 None:intergenic
GGCCAAGAAGGTTGTGAAAC+AGG 0.353292 2.3:-32005939 None:intergenic
GAACCAACAGGACTGACTTA+TGG 0.368553 2.3:+32006182 MS.gene60887:CDS
CAAGCTGGCCTTAATCTACT+TGG 0.385006 2.3:+32007286 MS.gene60887:CDS
TAATAAAGGTAGAAATGTTA+TGG 0.389081 2.3:+32007339 MS.gene60887:CDS
CTAACACTGATGGTTCCTAT+TGG 0.400945 2.3:+32006864 MS.gene60887:CDS
GGCACCTTCGTTGCTCAAAC+TGG 0.403001 2.3:+32006590 MS.gene60887:CDS
AGTATTAAATTGCAACATAA+AGG 0.403672 2.3:+32006038 MS.gene60887:CDS
ATTGTTTATGCTTCTTTGCA+TGG 0.404302 2.3:+32007247 MS.gene60887:CDS
GGGCGCCTACGACGGTGGAC+CGG 0.407167 2.3:+32006136 MS.gene60887:CDS
GCAAACACAGTCATCAATCT+AGG 0.408168 2.3:+32006071 MS.gene60887:CDS
CAATAGGAACCATCAGTGTT+AGG 0.411818 2.3:-32006863 None:intergenic
TTCAAACATGAAATTGATGA+AGG 0.414574 2.3:-32006626 None:intergenic
ACTTACCTTCCTCTGTCAAT+TGG 0.417119 2.3:+32006750 MS.gene60887:CDS
GCAAGAGATGATACTAATAA+AGG 0.425735 2.3:+32007325 MS.gene60887:CDS
CTTGTATAGTAATGCTCCAT+TGG 0.433338 2.3:-32006780 None:intergenic
GGTAGAAATGTTATGGATAT+GGG 0.435541 2.3:+32007346 MS.gene60887:CDS
GGATATGGGAAAATATGTAT+TGG 0.435630 2.3:+32007360 MS.gene60887:CDS
ACCAACAGGTCCAAAGGAAA+AGG 0.437204 2.3:+32007576 MS.gene60887:CDS
CTTCATCGGAATGTAAGTAT+AGG 0.445447 2.3:-32006724 None:intergenic
ACTTATGGATACTCTATGTT+AGG 0.451687 2.3:+32006197 MS.gene60887:CDS
CCTGTTCCAATAGCACAAAA+TGG 0.455151 2.3:+32006821 MS.gene60887:CDS
GGCGCCTACGACGGTGGACC+GGG 0.458088 2.3:+32006137 MS.gene60887:CDS
AATGTGGCTAAGGCATTGCC+TGG 0.460752 2.3:+32007487 MS.gene60887:CDS
CCTACAGCATGGTAGCCATT+AGG 0.461371 2.3:-32006395 None:intergenic
AATACTAGTGAGTAATCAAC+AGG 0.461578 2.3:-32006311 None:intergenic
GATATTTCAGGAAAGACTAA+TGG 0.462884 2.3:+32006275 MS.gene60887:CDS
GATATTAAATTAGGAAAAGC+AGG 0.463543 2.3:+32007058 MS.gene60887:CDS
GAAGGTGCCAACTCGAACAC+CGG 0.464880 2.3:-32006576 None:intergenic
TGTTGGTCCTTCCTCTCCCA+AGG 0.465771 2.3:-32007560 None:intergenic
TCTTTGTTTGCATTATCAGC+AGG 0.468164 2.3:-32006896 None:intergenic
TGCAAAGAAGCATAAACAAT+AGG 0.471114 2.3:-32007244 None:intergenic
CAGGTGAACATGTTGGTGAT+TGG 0.472172 2.3:+32007077 MS.gene60887:CDS
GTTGAATTATTTCAGGGAGT+GGG 0.475428 2.3:+32007426 MS.gene60887:CDS
ATAGGAATGGAGCGTTAACT+TGG 0.477931 2.3:-32006706 None:intergenic
GTTTGGGGCGCCTACGACGG+TGG 0.480695 2.3:+32006131 MS.gene60887:CDS
TGGTAGCCATTAGGTGCTAT+TGG 0.481801 2.3:-32006386 None:intergenic
TTGGGAGAGGAAGGACCAAC+AGG 0.483726 2.3:+32007562 MS.gene60887:CDS
CACATGACTTTGTTGATGGA+AGG 0.484098 2.3:-32006446 None:intergenic
GGTTGAATTATTTCAGGGAG+TGG 0.486043 2.3:+32007425 MS.gene60887:CDS
TGGCTACCAATAGCACCTAA+TGG 0.488722 2.3:+32006380 MS.gene60887:CDS
CAAAACTACTAGCATCACTT+TGG 0.490875 2.3:-32007204 None:intergenic
ACTGAGGCGCCTCGATTGCC+CGG 0.492573 2.3:-32006155 None:intergenic
GAGTTTACCAAAAGAAGCCT+TGG 0.494113 2.3:+32007543 MS.gene60887:CDS
TCCTTTCAATGGACCTGGAA+GGG 0.497466 2.3:+32007018 MS.gene60887:CDS
GCCCTTCCAGGTCCATTGAA+AGG 0.503272 2.3:-32007019 None:intergenic
GCCTCAGTTTATGAACCAAC+AGG 0.503883 2.3:+32006170 MS.gene60887:CDS
TGGTTATGTTCTTGTAGCAA+AGG 0.506215 2.3:+32006253 MS.gene60887:CDS
ATGATACCTTCTGGCCAAGA+AGG 0.506844 2.3:-32005951 None:intergenic
AGGTGAACATGTTGGTGATT+GGG 0.508342 2.3:+32007078 MS.gene60887:CDS
CTGAAATAATTCAACCATGC+TGG 0.508539 2.3:-32007418 None:intergenic
AGCATGGTTGAATTATTTCA+GGG 0.512489 2.3:+32007420 MS.gene60887:CDS
AGCAGGTAGGTCAAGCCAAT+AGG 0.513437 2.3:-32006879 None:intergenic
AGTTTACCAAAAGAAGCCTT+GGG 0.519013 2.3:+32007544 MS.gene60887:CDS
GTACATGTGAAACCGATGTA+TGG 0.520814 2.3:+32006959 MS.gene60887:CDS
ACAGTGATTTGGGGATCAAA+CGG 0.523270 2.3:+32006506 MS.gene60887:CDS
TCTAGATCAAAGTCAATATG+TGG 0.527060 2.3:-32007451 None:intergenic
ACCTTCGTTGCTCAAACTGG+TGG 0.531935 2.3:+32006593 MS.gene60887:CDS
AAACCATCTCTTAACTTCCC+AGG 0.534968 2.3:-32007505 None:intergenic
CCAACAGGTCCAAAGGAAAA+GGG 0.535094 2.3:+32007577 MS.gene60887:CDS
TCACAACCTTCTTGGCCAGA+AGG 0.539430 2.3:+32005945 MS.gene60887:CDS
CCATCAGTGTTAGGATCTTG+AGG 0.541983 2.3:-32006854 None:intergenic
TACTTCTCTCAACACAATGC+TGG 0.544284 2.3:+32007151 MS.gene60887:CDS
AAAGAGGCTTGTTGTTGGGT+TGG 0.546487 2.3:-32006229 None:intergenic
GGAAGGACCAACAGGTCCAA+AGG 0.546506 2.3:+32007570 MS.gene60887:CDS
ACCAAAAGAAGCCTTGGGAG+AGG 0.547085 2.3:+32007549 MS.gene60887:CDS
GACGGTGGACCGGGCAATCG+AGG 0.551257 2.3:+32006146 MS.gene60887:CDS
AAAGAAGCCTTGGGAGAGGA+AGG 0.551794 2.3:+32007553 MS.gene60887:CDS
ATCCTTTCAATGGACCTGGA+AGG 0.553889 2.3:+32007017 MS.gene60887:CDS
TCAAACATGAAATTGATGAA+GGG 0.554987 2.3:-32006625 None:intergenic
TGAATTGCAGAATGTGGCTA+AGG 0.555864 2.3:+32007477 MS.gene60887:CDS
TCGGAATGTAAGTATAGGAA+TGG 0.556685 2.3:-32006719 None:intergenic
ATTGCCCGGTCCACCGTCGT+AGG 0.557656 2.3:-32006141 None:intergenic
TTGAATTATTTCAGGGAGTG+GGG 0.560005 2.3:+32007427 MS.gene60887:CDS
CCTCAAGATCCTAACACTGA+TGG 0.563292 2.3:+32006854 MS.gene60887:CDS
AATAGAGGAATTAAAGCTCC+CGG 0.563330 2.3:+32006557 MS.gene60887:CDS
ATTCACTGATATTGCCATGT+GGG 0.565127 2.3:+32006988 MS.gene60887:CDS
GGAGCATTACTATACAAGAA+AGG 0.567167 2.3:+32006785 MS.gene60887:CDS
AAGGAAAAGGGTAATTGGAA+TGG 0.567477 2.3:+32007589 MS.gene60887:CDS
AACAAAGAGAGAGTCAAGAA+AGG 0.571578 2.3:+32006911 MS.gene60887:CDS
TATCCATAAGTCAGTCCTGT+TGG 0.574072 2.3:-32006185 None:intergenic
AAGCTGGCCTTAATCTACTT+GGG 0.575368 2.3:+32007287 MS.gene60887:CDS
GGAAGGTAAGTATTCTTCAT+CGG 0.581670 2.3:-32006738 None:intergenic
AAAGCTCCCGGTGTTCGAGT+TGG 0.582792 2.3:+32006569 MS.gene60887:CDS
CAGTGTTAGGATCTTGAGGA+AGG 0.583165 2.3:-32006850 None:intergenic
CATGCATCATATCCACAAGC+TGG 0.583723 2.3:+32007271 MS.gene60887:CDS
GTAGTGAATGTGGCGAGAGC+GGG 0.585956 2.3:-32005904 None:intergenic
GATGTTAGACCAATAAATAG+AGG 0.590723 2.3:+32006542 MS.gene60887:CDS
CTGAAGTTAAAGAGCCAGCA+TGG 0.591737 2.3:+32007404 MS.gene60887:CDS
TTGCCTGGGAAGTTAAGAGA+TGG 0.593085 2.3:+32007502 MS.gene60887:CDS
TGTTTGCATTATCAGCAGGT+AGG 0.599393 2.3:-32006892 None:intergenic
GGAAAAGCAGGTGAACATGT+TGG 0.601860 2.3:+32007070 MS.gene60887:CDS
AAAGTTTGGGGCGCCTACGA+CGG 0.602534 2.3:+32006128 MS.gene60887:CDS
CCTTCGTTGCTCAAACTGGT+GGG 0.608905 2.3:+32006594 MS.gene60887:CDS
CCCACCAGTTTGAGCAACGA+AGG 0.609155 2.3:-32006594 None:intergenic
GTGAATGTTCCTCCATACAT+CGG 0.616114 2.3:-32006971 None:intergenic
CCTAATGGCTACCATGCTGT+AGG 0.618336 2.3:+32006395 MS.gene60887:CDS
AGATATTGAATTGCAGAATG+TGG 0.619484 2.3:+32007471 MS.gene60887:CDS
AAGGTGCCAACTCGAACACC+GGG 0.623158 2.3:-32006575 None:intergenic
TCAACATTCAGCAAAGTTTG+GGG 0.632154 2.3:+32006116 MS.gene60887:CDS
ATTGGTAGCCAAATATACAA+AGG 0.637296 2.3:-32006368 None:intergenic
ATGTGGCTAAGGCATTGCCT+GGG 0.639158 2.3:+32007488 MS.gene60887:CDS
TTAAGAATAAGTAACTTCGA+TGG 0.643509 2.3:+32007112 MS.gene60887:CDS
AGTAGATTAAGGCCAGCTTG+TGG 0.643889 2.3:-32007283 None:intergenic
TCCTGTTGGTTCATAAACTG+AGG 0.647497 2.3:-32006171 None:intergenic
TGTAGTGAATGTGGCGAGAG+CGG 0.649291 2.3:-32005905 None:intergenic
ATTGAACACTGCATCCGCCA+CGG 0.658084 2.3:+32006331 MS.gene60887:CDS
GAATCGTCTACAGTGATTTG+GGG 0.659719 2.3:+32006497 MS.gene60887:CDS
GCTTAGATGTGTAGTGAATG+TGG 0.662600 2.3:-32005914 None:intergenic
CATGTGAAACCGATGTATGG+AGG 0.666811 2.3:+32006962 MS.gene60887:CDS
AGGAGAGTCTTGAGTAACCG+TGG 0.681322 2.3:-32006348 None:intergenic
CATTCACTGATATTGCCATG+TGG 0.686069 2.3:+32006987 MS.gene60887:CDS
ACTAGTGAGTAATCAACAGG+TGG 0.691530 2.3:-32006308 None:intergenic
ACGATTCACATTGCTCTGTG+AGG 0.692137 2.3:-32006481 None:intergenic
TGACAACATGACCTACAGCA+TGG 0.693224 2.3:-32006406 None:intergenic
AGAGTCTTGAGTAACCGTGG+CGG 0.757224 2.3:-32006345 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAGTTTATAGATATTAAATT+AGG + chr2.3:32007049-32007068 MS.gene60887:CDS 15.0%
!! AGTATTAAATTGCAACATAA+AGG + chr2.3:32006038-32006057 MS.gene60887:CDS 20.0%
!! TAATAAAGGTAGAAATGTTA+TGG + chr2.3:32007339-32007358 MS.gene60887:CDS 20.0%
!! TGACTAGAATATATTACAAA+TGG - chr2.3:32005999-32006018 None:intergenic 20.0%
!!! ATGGTTTTGAAAGTATTATT+AGG + chr2.3:32007521-32007540 MS.gene60887:CDS 20.0%
! GATATTAAATTAGGAAAAGC+AGG + chr2.3:32007058-32007077 MS.gene60887:CDS 25.0%
! GCTTTAATTCCTCTATTTAT+TGG - chr2.3:32006554-32006573 None:intergenic 25.0%
! TCAAACATGAAATTGATGAA+GGG - chr2.3:32006628-32006647 None:intergenic 25.0%
! TTAAGAATAAGTAACTTCGA+TGG + chr2.3:32007112-32007131 MS.gene60887:CDS 25.0%
! TTCAAACATGAAATTGATGA+AGG - chr2.3:32006629-32006648 None:intergenic 25.0%
!! TAAACAATAGGTCTTTTTGT+TGG - chr2.3:32007235-32007254 None:intergenic 25.0%
!!! GCATCAATTTGTTTTTTACT+AGG - chr2.3:32006680-32006699 None:intergenic 25.0%
AAGACTCTCCTTTGTATATT+TGG + chr2.3:32006360-32006379 MS.gene60887:CDS 30.0%
AATACTAGTGAGTAATCAAC+AGG - chr2.3:32006314-32006333 None:intergenic 30.0%
ACAAGAACATAACCAAAAAG+AGG - chr2.3:32006248-32006267 None:intergenic 30.0%
ACTTATGGATACTCTATGTT+AGG + chr2.3:32006197-32006216 MS.gene60887:CDS 30.0%
AGATATTGAATTGCAGAATG+TGG + chr2.3:32007471-32007490 MS.gene60887:CDS 30.0%
AGCATGGTTGAATTATTTCA+GGG + chr2.3:32007420-32007439 MS.gene60887:CDS 30.0%
AGGTAGAAATGTTATGGATA+TGG + chr2.3:32007345-32007364 MS.gene60887:CDS 30.0%
ATCAACATTCAGCAAAGTTT+GGG + chr2.3:32006115-32006134 MS.gene60887:CDS 30.0%
ATCACATAAATGATACCTTC+TGG - chr2.3:32005963-32005982 None:intergenic 30.0%
ATTGTTTATGCTTCTTTGCA+TGG + chr2.3:32007247-32007266 MS.gene60887:CDS 30.0%
CTTAATCTACTTGGGAAAAA+TGG + chr2.3:32007295-32007314 MS.gene60887:CDS 30.0%
GATATTTCAGGAAAGACTAA+TGG + chr2.3:32006275-32006294 MS.gene60887:CDS 30.0%
GATGTTAGACCAATAAATAG+AGG + chr2.3:32006542-32006561 MS.gene60887:CDS 30.0%
GCAAGAGATGATACTAATAA+AGG + chr2.3:32007325-32007344 MS.gene60887:CDS 30.0%
GGATATGGGAAAATATGTAT+TGG + chr2.3:32007360-32007379 MS.gene60887:CDS 30.0%
GGTAGAAATGTTATGGATAT+GGG + chr2.3:32007346-32007365 MS.gene60887:CDS 30.0%
TCTAGATCAAAGTCAATATG+TGG - chr2.3:32007454-32007473 None:intergenic 30.0%
TGGTTTCTGCAGAATATTTA+GGG + chr2.3:32007380-32007399 MS.gene60887:CDS 30.0%
TTGGTTTCTGCAGAATATTT+AGG + chr2.3:32007379-32007398 MS.gene60887:CDS 30.0%
! ATTGGTAGCCAAATATACAA+AGG - chr2.3:32006371-32006390 None:intergenic 30.0%
! CATTTTTCCCAAGTAGATTA+AGG - chr2.3:32007297-32007316 None:intergenic 30.0%
! TGCAAAGAAGCATAAACAAT+AGG - chr2.3:32007247-32007266 None:intergenic 30.0%
!!! TGGGTTTTTTATCCTTTCAA+TGG + chr2.3:32007007-32007026 MS.gene60887:CDS 30.0%
AAAAACCAATTGACAGAGGA+AGG - chr2.3:32006758-32006777 None:intergenic 35.0%
AACAAAGAGAGAGTCAAGAA+AGG + chr2.3:32006911-32006930 MS.gene60887:CDS 35.0%
AACACACATGACTTTGTTGA+TGG - chr2.3:32006453-32006472 None:intergenic 35.0%
AAGGAAAAGGGTAATTGGAA+TGG + chr2.3:32007589-32007608 MS.gene60887:CDS 35.0%
AATAGAGGAATTAAAGCTCC+CGG + chr2.3:32006557-32006576 MS.gene60887:CDS 35.0%
AGTTTACCAAAAGAAGCCTT+GGG + chr2.3:32007544-32007563 MS.gene60887:CDS 35.0%
ATTCACTGATATTGCCATGT+GGG + chr2.3:32006988-32007007 MS.gene60887:CDS 35.0%
CAAAACTACTAGCATCACTT+TGG - chr2.3:32007207-32007226 None:intergenic 35.0%
CAGCATGGTTGAATTATTTC+AGG + chr2.3:32007419-32007438 MS.gene60887:CDS 35.0%
CATCAACATTCAGCAAAGTT+TGG + chr2.3:32006114-32006133 MS.gene60887:CDS 35.0%
CTGAAATAATTCAACCATGC+TGG - chr2.3:32007421-32007440 None:intergenic 35.0%
CTTCATCGGAATGTAAGTAT+AGG - chr2.3:32006727-32006746 None:intergenic 35.0%
CTTGTAGCAAAGGATATTTC+AGG + chr2.3:32006263-32006282 MS.gene60887:CDS 35.0%
CTTGTATAGTAATGCTCCAT+TGG - chr2.3:32006783-32006802 None:intergenic 35.0%
GAAAGGATAAAAAACCCACA+TGG - chr2.3:32007005-32007024 None:intergenic 35.0%
GGAAGGTAAGTATTCTTCAT+CGG - chr2.3:32006741-32006760 None:intergenic 35.0%
GTAACTTCGATGGTCAATTA+TGG + chr2.3:32007122-32007141 MS.gene60887:CDS 35.0%
GTTGAATTATTTCAGGGAGT+GGG + chr2.3:32007426-32007445 MS.gene60887:CDS 35.0%
TCAACATTCAGCAAAGTTTG+GGG + chr2.3:32006116-32006135 MS.gene60887:CDS 35.0%
TCGGAATGTAAGTATAGGAA+TGG - chr2.3:32006722-32006741 None:intergenic 35.0%
TGAATCGTCTACAGTGATTT+GGG + chr2.3:32006496-32006515 MS.gene60887:CDS 35.0%
TGGTTATGTTCTTGTAGCAA+AGG + chr2.3:32006253-32006272 MS.gene60887:CDS 35.0%
TTGAATTATTTCAGGGAGTG+GGG + chr2.3:32007427-32007446 MS.gene60887:CDS 35.0%
! GGAAGGTTTATCTTGTGTTT+TGG - chr2.3:32006432-32006451 None:intergenic 35.0%
! GGAGCATTACTATACAAGAA+AGG + chr2.3:32006785-32006804 MS.gene60887:CDS 35.0%
! TCTTTGTTTGCATTATCAGC+AGG - chr2.3:32006899-32006918 None:intergenic 35.0%
! TTAGCACCATTTTGTGCTAT+TGG - chr2.3:32006830-32006849 None:intergenic 35.0%
! TTCCAATTACCCTTTTCCTT+TGG - chr2.3:32007589-32007608 None:intergenic 35.0%
! TTTTATCCTTTCAATGGACC+TGG + chr2.3:32007013-32007032 MS.gene60887:CDS 35.0%
!!! GTCAATTGGTTTTTCTCCAA+TGG + chr2.3:32006764-32006783 MS.gene60887:CDS 35.0%
AAACCATCTCTTAACTTCCC+AGG - chr2.3:32007508-32007527 None:intergenic 40.0%
ACTAGTGAGTAATCAACAGG+TGG - chr2.3:32006311-32006330 None:intergenic 40.0%
ACTTACCTTCCTCTGTCAAT+TGG + chr2.3:32006750-32006769 MS.gene60887:CDS 40.0%
CAATAGGAACCATCAGTGTT+AGG - chr2.3:32006866-32006885 None:intergenic 40.0%
CATTCACTGATATTGCCATG+TGG + chr2.3:32006987-32007006 MS.gene60887:CDS 40.0%
CCTGTTCCAATAGCACAAAA+TGG + chr2.3:32006821-32006840 MS.gene60887:CDS 40.0%
CTAACACTGATGGTTCCTAT+TGG + chr2.3:32006864-32006883 MS.gene60887:CDS 40.0%
CTACTTGGGAAAAATGGAGT+AGG + chr2.3:32007301-32007320 MS.gene60887:CDS 40.0%
CTCAACACAATGCTGGTAAA+TGG + chr2.3:32007158-32007177 MS.gene60887:CDS 40.0%
GAATCGTCTACAGTGATTTG+GGG + chr2.3:32006497-32006516 MS.gene60887:CDS 40.0%
GAGTTTACCAAAAGAAGCCT+TGG + chr2.3:32007543-32007562 MS.gene60887:CDS 40.0%
GATGACTGTGTTTGCAAAAC+TGG - chr2.3:32006066-32006085 None:intergenic 40.0%
GCAAACACAGTCATCAATCT+AGG + chr2.3:32006071-32006090 MS.gene60887:CDS 40.0%
GCTTAGATGTGTAGTGAATG+TGG - chr2.3:32005917-32005936 None:intergenic 40.0%
GGAGAAAAACCAATTGACAG+AGG - chr2.3:32006762-32006781 None:intergenic 40.0%
GGTTGAATTATTTCAGGGAG+TGG + chr2.3:32007425-32007444 MS.gene60887:CDS 40.0%
GTACATGTGAAACCGATGTA+TGG + chr2.3:32006959-32006978 MS.gene60887:CDS 40.0%
GTCCAAAGGAAAAGGGTAAT+TGG + chr2.3:32007584-32007603 MS.gene60887:CDS 40.0%
GTGAATCGTCTACAGTGATT+TGG + chr2.3:32006495-32006514 MS.gene60887:CDS 40.0%
GTGAATGTTCCTCCATACAT+CGG - chr2.3:32006974-32006993 None:intergenic 40.0%
TACTTCTCTCAACACAATGC+TGG + chr2.3:32007151-32007170 MS.gene60887:CDS 40.0%
TATCCATAAGTCAGTCCTGT+TGG - chr2.3:32006188-32006207 None:intergenic 40.0%
TGAATTGCAGAATGTGGCTA+AGG + chr2.3:32007477-32007496 MS.gene60887:CDS 40.0%
TGTTTGCATTATCAGCAGGT+AGG - chr2.3:32006895-32006914 None:intergenic 40.0%
TTCCTGTTTCACAACCTTCT+TGG + chr2.3:32005937-32005956 MS.gene60887:CDS 40.0%
! ACAGTGATTTGGGGATCAAA+CGG + chr2.3:32006506-32006525 MS.gene60887:CDS 40.0%
! ATAGGAATGGAGCGTTAACT+TGG - chr2.3:32006709-32006728 None:intergenic 40.0%
! CACATGACTTTGTTGATGGA+AGG - chr2.3:32006449-32006468 None:intergenic 40.0%
!! AAGCTGGCCTTAATCTACTT+GGG + chr2.3:32007287-32007306 MS.gene60887:CDS 40.0%
!! ACCAAAAAGAGGCTTGTTGT+TGG - chr2.3:32006237-32006256 None:intergenic 40.0%
!! AGGTGAACATGTTGGTGATT+GGG + chr2.3:32007078-32007097 MS.gene60887:CDS 40.0%
!! CCAAAAAGAGGCTTGTTGTT+GGG - chr2.3:32006236-32006255 None:intergenic 40.0%
!! CCATTTTGTGCTATTGGAAC+AGG - chr2.3:32006824-32006843 None:intergenic 40.0%
!! TCCTGTTGGTTCATAAACTG+AGG - chr2.3:32006174-32006193 None:intergenic 40.0%
AACTCTACTTTAGCCCTTCC+AGG - chr2.3:32007034-32007053 None:intergenic 45.0%
ACCAACAGGTCCAAAGGAAA+AGG + chr2.3:32007576-32007595 MS.gene60887:CDS 45.0%
ACGATTCACATTGCTCTGTG+AGG - chr2.3:32006484-32006503 None:intergenic 45.0%
AGTAGATTAAGGCCAGCTTG+TGG - chr2.3:32007286-32007305 None:intergenic 45.0%
ATCCTTTCAATGGACCTGGA+AGG + chr2.3:32007017-32007036 MS.gene60887:CDS 45.0%
ATGATACCTTCTGGCCAAGA+AGG - chr2.3:32005954-32005973 None:intergenic 45.0%
CAGTGTTAGGATCTTGAGGA+AGG - chr2.3:32006853-32006872 None:intergenic 45.0%
CATGCATCATATCCACAAGC+TGG + chr2.3:32007271-32007290 MS.gene60887:CDS 45.0%
CATGTGAAACCGATGTATGG+AGG + chr2.3:32006962-32006981 MS.gene60887:CDS 45.0%
CCAACAGGTCCAAAGGAAAA+GGG + chr2.3:32007577-32007596 MS.gene60887:CDS 45.0%
CCATCAGTGTTAGGATCTTG+AGG - chr2.3:32006857-32006876 None:intergenic 45.0%
CCTCAAGATCCTAACACTGA+TGG + chr2.3:32006854-32006873 MS.gene60887:CDS 45.0%
CTGAAGTTAAAGAGCCAGCA+TGG + chr2.3:32007404-32007423 MS.gene60887:CDS 45.0%
GCCTCAGTTTATGAACCAAC+AGG + chr2.3:32006170-32006189 MS.gene60887:CDS 45.0%
GGAAAAGCAGGTGAACATGT+TGG + chr2.3:32007070-32007089 MS.gene60887:CDS 45.0%
TCCTTTCAATGGACCTGGAA+GGG + chr2.3:32007018-32007037 MS.gene60887:CDS 45.0%
TGACAACATGACCTACAGCA+TGG - chr2.3:32006409-32006428 None:intergenic 45.0%
TGGCTACCAATAGCACCTAA+TGG + chr2.3:32006380-32006399 MS.gene60887:CDS 45.0%
TTGCCTGGGAAGTTAAGAGA+TGG + chr2.3:32007502-32007521 MS.gene60887:CDS 45.0%
! CCCAACAACAAGCCTCTTTT+TGG + chr2.3:32006233-32006252 MS.gene60887:CDS 45.0%
! GAACCAACAGGACTGACTTA+TGG + chr2.3:32006182-32006201 MS.gene60887:CDS 45.0%
! TGGTAGCCATTAGGTGCTAT+TGG - chr2.3:32006389-32006408 None:intergenic 45.0%
!! AAAGAGGCTTGTTGTTGGGT+TGG - chr2.3:32006232-32006251 None:intergenic 45.0%
!! CAAGCTGGCCTTAATCTACT+TGG + chr2.3:32007286-32007305 MS.gene60887:CDS 45.0%
!! CAGGTGAACATGTTGGTGAT+TGG + chr2.3:32007077-32007096 MS.gene60887:CDS 45.0%
AAAGAAGCCTTGGGAGAGGA+AGG + chr2.3:32007553-32007572 MS.gene60887:CDS 50.0%
ACCAAAAGAAGCCTTGGGAG+AGG + chr2.3:32007549-32007568 MS.gene60887:CDS 50.0%
ACCTTCGTTGCTCAAACTGG+TGG + chr2.3:32006593-32006612 MS.gene60887:CDS 50.0%
AGAGTCTTGAGTAACCGTGG+CGG - chr2.3:32006348-32006367 None:intergenic 50.0%
AGCAGGTAGGTCAAGCCAAT+AGG - chr2.3:32006882-32006901 None:intergenic 50.0%
AGGAGAGTCTTGAGTAACCG+TGG - chr2.3:32006351-32006370 None:intergenic 50.0%
ATTGAACACTGCATCCGCCA+CGG + chr2.3:32006331-32006350 MS.gene60887:CDS 50.0%
CCTAATGGCTACCATGCTGT+AGG + chr2.3:32006395-32006414 MS.gene60887:CDS 50.0%
CCTACAGCATGGTAGCCATT+AGG - chr2.3:32006398-32006417 None:intergenic 50.0%
CCTTCGTTGCTCAAACTGGT+GGG + chr2.3:32006594-32006613 MS.gene60887:CDS 50.0%
GGCCAAGAAGGTTGTGAAAC+AGG - chr2.3:32005942-32005961 None:intergenic 50.0%
TCACAACCTTCTTGGCCAGA+AGG + chr2.3:32005945-32005964 MS.gene60887:CDS 50.0%
TGTAGTGAATGTGGCGAGAG+CGG - chr2.3:32005908-32005927 None:intergenic 50.0%
! CCCTTTTCCTTTGGACCTGT+TGG - chr2.3:32007580-32007599 None:intergenic 50.0%
! TCCTCTCCCAAGGCTTCTTT+TGG - chr2.3:32007553-32007572 None:intergenic 50.0%
!! AATGTGGCTAAGGCATTGCC+TGG + chr2.3:32007487-32007506 MS.gene60887:CDS 50.0%
!! ATGTGGCTAAGGCATTGCCT+GGG + chr2.3:32007488-32007507 MS.gene60887:CDS 50.0%
AAAGCTCCCGGTGTTCGAGT+TGG + chr2.3:32006569-32006588 MS.gene60887:CDS 55.0%
CCCACCAGTTTGAGCAACGA+AGG - chr2.3:32006597-32006616 None:intergenic 55.0%
GCCCTTCCAGGTCCATTGAA+AGG - chr2.3:32007022-32007041 None:intergenic 55.0%
GGAAGGACCAACAGGTCCAA+AGG + chr2.3:32007570-32007589 MS.gene60887:CDS 55.0%
GGCACCTTCGTTGCTCAAAC+TGG + chr2.3:32006590-32006609 MS.gene60887:CDS 55.0%
GTAGTGAATGTGGCGAGAGC+GGG - chr2.3:32005907-32005926 None:intergenic 55.0%
TGTTGGTCCTTCCTCTCCCA+AGG - chr2.3:32007563-32007582 None:intergenic 55.0%
TTGGGAGAGGAAGGACCAAC+AGG + chr2.3:32007562-32007581 MS.gene60887:CDS 55.0%
! AAAGTTTGGGGCGCCTACGA+CGG + chr2.3:32006128-32006147 MS.gene60887:CDS 55.0%
! AAGGTGCCAACTCGAACACC+GGG - chr2.3:32006578-32006597 None:intergenic 55.0%
!! GAAGGTGCCAACTCGAACAC+CGG - chr2.3:32006579-32006598 None:intergenic 55.0%
ATTGCCCGGTCCACCGTCGT+AGG - chr2.3:32006144-32006163 None:intergenic 65.0%
! ACTGAGGCGCCTCGATTGCC+CGG - chr2.3:32006158-32006177 None:intergenic 65.0%
GACGGTGGACCGGGCAATCG+AGG + chr2.3:32006146-32006165 MS.gene60887:CDS 70.0%
! GTTTGGGGCGCCTACGACGG+TGG + chr2.3:32006131-32006150 MS.gene60887:CDS 70.0%
GGCGCCTACGACGGTGGACC+GGG + chr2.3:32006137-32006156 MS.gene60887:CDS 75.0%
GGGCGCCTACGACGGTGGAC+CGG + chr2.3:32006136-32006155 MS.gene60887:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 32005883 32007624 32005883 ID=MS.gene60887
chr2.3 mRNA 32005883 32007624 32005883 ID=MS.gene60887.t1;Parent=MS.gene60887
chr2.3 exon 32005883 32005966 32005883 ID=MS.gene60887.t1.exon1;Parent=MS.gene60887.t1
chr2.3 CDS 32005883 32005966 32005883 ID=cds.MS.gene60887.t1;Parent=MS.gene60887.t1
chr2.3 exon 32006026 32007624 32006026 ID=MS.gene60887.t1.exon2;Parent=MS.gene60887.t1
chr2.3 CDS 32006026 32007624 32006026 ID=cds.MS.gene60887.t1;Parent=MS.gene60887.t1
Gene Sequence

>MS.gene60887

ATGACTTCAAGAAAAAGCGATCCCGCTCTCGCCACATTCACTACACATCTAAGCTTCCTGTTTCACAACCTTCTTGGCCAGAAGGTATCATTTATGTGATTTCAATTTTCTGTCCATTTGTAATATATTCTAGTCAATGTAAGCTAACTATAGCAAGTATTAAATTGCAACATAAAGGTACCAGTTTTGCAAACACAGTCATCAATCTAGGTGAGCTGCATGTGATGCAAGCATCAACATTCAGCAAAGTTTGGGGCGCCTACGACGGTGGACCGGGCAATCGAGGCGCCTCAGTTTATGAACCAACAGGACTGACTTATGGATACTCTATGTTAGGCAGTTACAGCCAACCCAACAACAAGCCTCTTTTTGGTTATGTTCTTGTAGCAAAGGATATTTCAGGAAAGACTAATGGCACTTTAAAGCCACCTGTTGATTACTCACTAGTATTGAACACTGCATCCGCCACGGTTACTCAAGACTCTCCTTTGTATATTTGGCTACCAATAGCACCTAATGGCTACCATGCTGTAGGTCATGTTGTCACCAAAACACAAGATAAACCTTCCATCAACAAAGTCATGTGTGTTAGATCAGACCTCACAGAGCAATGTGAATCGTCTACAGTGATTTGGGGATCAAACGGCTTAAATTTTTACGATGTTAGACCAATAAATAGAGGAATTAAAGCTCCCGGTGTTCGAGTTGGCACCTTCGTTGCTCAAACTGGTGGGAATATTAACCCTTCATCAATTTCATGTTTGAAGAATGTTGATTCTATTACACAAATCATGCCTAGTAAAAAACAAATTGATGCACTACTCCAAGTTAACGCTCCATTCCTATACTTACATTCCGATGAAGAATACTTACCTTCCTCTGTCAATTGGTTTTTCTCCAATGGAGCATTACTATACAAGAAAGGTGAAGAATCAAATCCTGTTCCAATAGCACAAAATGGTGCTAACCTTCCTCAAGATCCTAACACTGATGGTTCCTATTGGCTTGACCTACCTGCTGATAATGCAAACAAAGAGAGAGTCAAGAAAGGTAATCTACAAAGTGCAGAATCTTACGTACATGTGAAACCGATGTATGGAGGAACATTCACTGATATTGCCATGTGGGTTTTTTATCCTTTCAATGGACCTGGAAGGGCTAAAGTAGAGTTTATAGATATTAAATTAGGAAAAGCAGGTGAACATGTTGGTGATTGGGAGCATGTGACATTAAGAATAAGTAACTTCGATGGTCAATTATGGCATATCTACTTCTCTCAACACAATGCTGGTAAATGGATTGATTCATCTCAACTTGAGTTCCAAAGTGATGCTAGTAGTTTTGATTTTCCAACAAAAAGACCTATTGTTTATGCTTCTTTGCATGGTCATGCATCATATCCACAAGCTGGCCTTAATCTACTTGGGAAAAATGGAGTAGGTGCAAGAGATGATACTAATAAAGGTAGAAATGTTATGGATATGGGAAAATATGTATTGGTTTCTGCAGAATATTTAGGGTCTGAAGTTAAAGAGCCAGCATGGTTGAATTATTTCAGGGAGTGGGGTCCACATATTGACTTTGATCTAGATATTGAATTGCAGAATGTGGCTAAGGCATTGCCTGGGAAGTTAAGAGATGGTTTTGAAAGTATTATTAGGAGTTTACCAAAAGAAGCCTTGGGAGAGGAAGGACCAACAGGTCCAAAGGAAAAGGGTAATTGGAATGGTGATGAAGTTTGA
Protein sequence

>MS.gene60887.t1

MTSRKSDPALATFTTHLSFLFHNLLGQKLTIASIKLQHKGTSFANTVINLGELHVMQASTFSKVWGAYDGGPGNRGASVYEPTGLTYGYSMLGSYSQPNNKPLFGYVLVAKDISGKTNGTLKPPVDYSLVLNTASATVTQDSPLYIWLPIAPNGYHAVGHVVTKTQDKPSINKVMCVRSDLTEQCESSTVIWGSNGLNFYDVRPINRGIKAPGVRVGTFVAQTGGNINPSSISCLKNVDSITQIMPSKKQIDALLQVNAPFLYLHSDEEYLPSSVNWFFSNGALLYKKGEESNPVPIAQNGANLPQDPNTDGSYWLDLPADNANKERVKKGNLQSAESYVHVKPMYGGTFTDIAMWVFYPFNGPGRAKVEFIDIKLGKAGEHVGDWEHVTLRISNFDGQLWHIYFSQHNAGKWIDSSQLEFQSDASSFDFPTKRPIVYASLHGHASYPQAGLNLLGKNGVGARDDTNKGRNVMDMGKYVLVSAEYLGSEVKEPAWLNYFREWGPHIDFDLDIELQNVAKALPGKLRDGFESIIRSLPKEALGEEGPTGPKEKGNWNGDEV