AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000412

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000412.t1 MTR_8g096060 95.847 313 10 1 1 313 1 310 0.0 615
MS.gene000412.t1 MTR_3g098470 83.067 313 51 1 1 313 1 311 0.0 556
MS.gene000412.t1 MTR_1g031180 73.826 298 78 0 16 313 19 316 6.26e-172 479
MS.gene000412.t1 MTR_4g087460 41.017 295 156 8 31 313 16 304 3.12e-70 221
MS.gene000412.t1 MTR_8g032000 38.644 295 144 5 39 307 9 292 7.06e-64 204
MS.gene000412.t1 MTR_8g032010 38.047 297 147 6 35 305 5 290 9.81e-63 201
MS.gene000412.t1 MTR_8g031960 39.000 300 145 6 38 311 9 296 8.79e-62 198
MS.gene000412.t1 MTR_8g031980 37.954 303 150 6 36 312 6 296 1.86e-61 197
MS.gene000412.t1 MTR_8g031990 36.393 305 157 5 35 313 5 298 3.55e-61 197
MS.gene000412.t1 MTR_7g076660 39.446 289 150 6 39 313 9 286 9.24e-61 195
MS.gene000412.t1 MTR_7g076660 39.446 289 150 6 39 313 10 287 1.22e-60 195
MS.gene000412.t1 MTR_7g076655 39.792 289 149 6 39 313 9 286 2.77e-60 194
MS.gene000412.t1 MTR_3g079320 37.748 302 149 5 33 305 7 298 3.17e-60 194
MS.gene000412.t1 MTR_8g031910 40.893 291 144 6 34 308 4 282 6.55e-60 193
MS.gene000412.t1 MTR_8g032020 37.417 302 151 6 39 313 10 300 9.22e-59 191
MS.gene000412.t1 MTR_5g062280 38.158 304 152 6 34 313 4 295 8.35e-58 188
MS.gene000412.t1 MTR_8g031940 39.796 294 139 8 39 305 9 291 6.33e-57 186
MS.gene000412.t1 MTR_8g031900 35.135 296 160 5 39 313 9 293 7.96e-57 186
MS.gene000412.t1 MTR_8g031630 37.542 301 150 9 39 313 9 297 9.89e-56 183
MS.gene000412.t1 MTR_8g031610 37.333 300 151 8 39 313 7 294 8.58e-55 180
MS.gene000412.t1 MTR_8g031650 37.333 300 150 9 40 313 104 391 6.04e-54 181
MS.gene000412.t1 MTR_8g031650 37.209 129 70 4 179 302 2 124 2.42e-14 73.2
MS.gene000412.t1 MTR_8g031600 36.928 306 150 7 37 313 7 298 4.05e-53 176
MS.gene000412.t1 MTR_8g031650 37.333 300 150 9 40 313 270 557 1.14e-52 181
MS.gene000412.t1 MTR_8g031650 38.225 293 141 9 39 302 9 290 2.31e-51 178
MS.gene000412.t1 MTR_7g076660 37.868 272 144 6 56 313 2 262 3.40e-52 172
MS.gene000412.t1 MTR_7g076665 35.117 299 158 6 39 313 9 295 1.22e-49 167
MS.gene000412.t1 MTR_7g106800 35.816 282 132 7 39 313 11 250 3.75e-46 157
MS.gene000412.t1 MTR_8g031850 32.130 277 156 5 39 313 9 255 1.56e-43 150
MS.gene000412.t1 MTR_5g083430 36.224 196 113 4 118 313 1 184 1.88e-34 124
MS.gene000412.t1 MTR_8g032030 39.665 179 80 5 36 187 6 183 1.57e-31 118
MS.gene000412.t1 MTR_4g105310 27.211 294 156 11 39 310 51 308 4.15e-14 72.4
MS.gene000412.t1 MTR_3g077410 26.531 294 158 11 39 310 40 297 4.03e-13 69.3
MS.gene000412.t1 MTR_4g105310 28.926 242 129 8 39 273 51 256 7.87e-13 67.8
MS.gene000412.t1 MTR_3g068070 35.374 147 86 4 31 172 12 154 1.24e-12 67.4
MS.gene000412.t1 MTR_6g053550 26.936 297 149 12 39 307 40 296 6.81e-12 65.5
MS.gene000412.t1 MTR_8g442750 31.973 147 93 2 35 174 15 161 1.27e-11 64.7
MS.gene000412.t1 MTR_7g063390 32.886 149 95 4 30 175 12 158 4.36e-11 62.8
MS.gene000412.t1 MTR_7g063360 36.601 153 84 7 30 175 12 158 9.27e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000412.t1 AT5G51030 70.927 313 91 0 1 313 2 314 9.34e-177 491
MS.gene000412.t1 AT5G61830 68.671 316 96 2 1 313 1 316 2.84e-162 454
MS.gene000412.t1 AT5G61830 68.671 316 96 2 1 313 1 316 2.84e-162 454
MS.gene000412.t1 AT3G59710 42.807 285 148 6 25 305 7 280 1.94e-76 236
MS.gene000412.t1 AT3G59710 42.908 282 146 6 28 305 39 309 4.63e-75 233
MS.gene000412.t1 AT3G59710 42.254 284 149 6 26 305 18 290 9.79e-74 229
MS.gene000412.t1 AT3G61220 41.077 297 141 8 39 312 9 294 6.79e-63 201
MS.gene000412.t1 AT3G61220 41.077 297 141 8 39 312 16 301 1.52e-62 201
MS.gene000412.t1 AT3G61220 41.077 297 141 8 39 312 40 325 2.89e-62 201
MS.gene000412.t1 AT2G24190 39.057 297 147 7 39 312 9 294 4.60e-61 196
MS.gene000412.t1 AT2G24190 39.057 297 147 7 39 312 14 299 6.17e-61 196
MS.gene000412.t1 AT1G01800 38.206 301 150 8 38 313 6 295 1.39e-58 190
MS.gene000412.t1 AT1G01800 37.705 183 103 3 131 313 89 260 1.26e-32 122
MS.gene000412.t1 AT5G50130 28.296 311 160 15 27 312 27 299 4.13e-19 86.7
MS.gene000412.t1 AT5G50130 29.054 296 148 15 41 312 8 265 3.10e-17 80.9
MS.gene000412.t1 AT5G50130 29.297 256 134 12 27 273 27 244 4.49e-17 79.7
MS.gene000412.t1 AT5G50130 29.183 257 134 12 27 273 27 245 5.08e-17 79.7
MS.gene000412.t1 AT5G50130 28.956 297 148 15 41 312 8 266 5.21e-17 80.5
MS.gene000412.t1 AT5G50130 29.297 256 134 12 27 273 27 244 1.99e-16 79.0
MS.gene000412.t1 AT5G50130 29.183 257 134 12 27 273 27 245 2.76e-16 78.6
MS.gene000412.t1 AT4G23420 29.352 293 145 11 39 307 49 303 6.77e-16 77.4
MS.gene000412.t1 AT4G23420 29.352 293 145 11 39 307 32 286 2.26e-15 75.9
MS.gene000412.t1 AT4G23420 29.352 293 145 11 39 307 32 286 2.26e-15 75.9
MS.gene000412.t1 AT5G50130 30.165 242 122 12 41 273 8 211 2.96e-14 72.4
MS.gene000412.t1 AT5G50130 30.041 243 122 12 41 273 8 212 3.84e-14 72.0
MS.gene000412.t1 AT4G23430 27.645 293 150 11 39 307 32 286 1.05e-12 68.2
MS.gene000412.t1 AT4G23430 27.304 293 149 11 39 307 32 284 3.20e-12 66.6
MS.gene000412.t1 AT4G23420 28.623 276 141 10 39 300 32 265 1.91e-11 63.9
MS.gene000412.t1 AT4G23420 35.374 147 82 4 39 176 32 174 1.93e-11 63.9
MS.gene000412.t1 AT2G29350 30.233 172 108 3 28 187 9 180 8.91e-11 62.0
MS.gene000412.t1 AT2G29350 30.233 172 108 3 28 187 9 180 9.25e-11 61.2

Find 64 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGGGATTAATTTCAATTT+TGG 0.065690 4.3:-7293755 MS.gene000412:CDS
GTTATTGATACAAATTATTT+TGG 0.142059 4.3:-7293701 MS.gene000412:CDS
TAATGCTTATACAAGATTTA+TGG 0.146212 4.3:-7293231 MS.gene000412:CDS
TAATTGCTTGTTCAGGAATA+AGG 0.220393 4.3:+7293069 None:intergenic
GCAGCAGACGCCTTCATTAA+TGG 0.230922 4.3:+7293650 None:intergenic
CCAATTCTCTGAGTGGTTAA+AGG 0.248625 4.3:-7293937 MS.gene000412:CDS
ACTGGTGGAAATAGAGGAAT+TGG 0.276887 4.3:-7294116 MS.gene000412:CDS
CTATGGTGGCTTAAATATTC+TGG 0.279719 4.3:-7293910 MS.gene000412:intron
CCTTTAACCACTCAGAGAAT+TGG 0.295244 4.3:+7293937 None:intergenic
AACCTTTGGTGATCAGAATA+AGG 0.298129 4.3:+7294503 None:intergenic
ATTCCTGAACAAGCAATTAC+AGG 0.309492 4.3:-7293065 MS.gene000412:CDS
TGTTGGCGTTGAATCAATTA+AGG 0.322106 4.3:-7294027 MS.gene000412:CDS
CATATATAGGTGGTGGTCTA+AGG 0.322319 4.3:-7294156 MS.gene000412:intron
TTTCCTGTAATTGCTTGTTC+AGG 0.345287 4.3:+7293062 None:intergenic
GTTGAGGACGGTGCTGATAC+TGG 0.352498 4.3:-7293104 MS.gene000412:CDS
GAAACAATTGCTGTGGTTAC+TGG 0.356872 4.3:-7294134 MS.gene000412:CDS
TCGCGTCTAGGTCGACTAAA+TGG 0.361109 4.3:-7293602 MS.gene000412:CDS
CATAGCTTCAATCATACGTT+TGG 0.371958 4.3:+7293676 None:intergenic
TGGGTTAAAACAGCTCTAAC+AGG 0.375689 4.3:-7293146 MS.gene000412:CDS
CTTTAACCACTCAGAGAATT+GGG 0.379720 4.3:+7293938 None:intergenic
CTTTCAGAGGAACTTATTGA+TGG 0.393217 4.3:-7293353 MS.gene000412:CDS
TTTATTAATTGTTATTGTCC+TGG 0.406931 4.3:-7293170 MS.gene000412:CDS
AATTGCTTGTTCAGGAATAA+GGG 0.408591 4.3:+7293070 None:intergenic
TAATTGTTATTGTCCTGGTT+GGG 0.411270 4.3:-7293165 MS.gene000412:CDS
TTAATTGTTATTGTCCTGGT+TGG 0.424909 4.3:-7293166 MS.gene000412:CDS
TCAAGACAACTTGCTGATCA+TGG 0.429903 4.3:-7294083 MS.gene000412:CDS
AGAGGATGGAACTTGGAAAT+CGG 0.442067 4.3:-7293303 MS.gene000412:CDS
GAGTGGTTAAAGGAAAACTA+TGG 0.450433 4.3:-7293927 MS.gene000412:CDS
ACGGTGCTGATACTGGAGTT+TGG 0.450987 4.3:-7293097 MS.gene000412:CDS
ACCTTTGGTGATCAGAATAA+GGG 0.466845 4.3:+7294504 None:intergenic
ATCAAGTAGAGGATGGAACT+TGG 0.475460 4.3:-7293310 MS.gene000412:CDS
CTGGTGGAAATAGAGGAATT+GGG 0.480536 4.3:-7294115 MS.gene000412:CDS
GAGGATGGAACTTGGAAATC+GGG 0.486517 4.3:-7293302 MS.gene000412:CDS
TTAATGAAGGCGTCTGCTGC+TGG 0.492869 4.3:-7293647 MS.gene000412:CDS
TTCAGTTGAAAATGCTCATA+CGG 0.503882 4.3:-7293723 MS.gene000412:CDS
AGCTATGATTCCATTAATGA+AGG 0.504391 4.3:-7293660 MS.gene000412:CDS
TATTTGCAGGTAAACAATGC+AGG 0.514024 4.3:-7293773 MS.gene000412:intron
GTAAATGTTAGCTCGCGTCT+AGG 0.519334 4.3:-7293614 MS.gene000412:CDS
ACAGCTCTAACAGGTTATGC+AGG 0.530819 4.3:-7293137 MS.gene000412:CDS
GAGTAGTCGGTAAATATTCG+AGG 0.558560 4.3:+7293272 None:intergenic
TTTCTACATCAAGTAGAGGA+TGG 0.561046 4.3:-7293317 MS.gene000412:CDS
GAACTTGGAAATCGGGAGGA+TGG 0.561877 4.3:-7293295 MS.gene000412:CDS
TCAATTAAGGTCTTGCAAGA+AGG 0.563494 4.3:-7294014 MS.gene000412:CDS
ACAACAAGAGATGCTAATGT+TGG 0.566475 4.3:-7294044 MS.gene000412:CDS
TGCAGGAAGTGTCTCAGTTG+AGG 0.575602 4.3:-7293120 MS.gene000412:CDS
ATTTGCAGGTAAACAATGCA+GGG 0.577061 4.3:-7293772 MS.gene000412:intron
TCCCTTATTCTGATCACCAA+AGG 0.577104 4.3:-7294505 MS.gene000412:intron
TAGTTTCGACAACGAGTAGT+CGG 0.587214 4.3:+7293259 None:intergenic
CCACTCAGAGAATTGGGTGA+TGG 0.590667 4.3:+7293944 None:intergenic
ATTAAGGTCTTGCAAGAAGG+TGG 0.604917 4.3:-7294011 MS.gene000412:CDS
AAACTTTCAGATAGACCTGA+GGG 0.619922 4.3:-7293203 MS.gene000412:CDS
GTGGTTACTGGTGGAAATAG+AGG 0.620891 4.3:-7294122 MS.gene000412:CDS
GTCTAAGGAAACAATTGCTG+TGG 0.624623 4.3:-7294141 MS.gene000412:CDS
ACAATTGCTGTGGTTACTGG+TGG 0.637431 4.3:-7294131 MS.gene000412:CDS
ATGAAGGCGTCTGCTGCTGG+TGG 0.639427 4.3:-7293644 MS.gene000412:CDS
CAATGTGGATAATCTTTCAG+AGG 0.643765 4.3:-7293366 MS.gene000412:CDS
GGAAGTGTCTCAGTTGAGGA+CGG 0.650718 4.3:-7293116 MS.gene000412:CDS
GATGGAACTTGGAAATCGGG+AGG 0.664292 4.3:-7293299 MS.gene000412:CDS
CCATCACCCAATTCTCTGAG+TGG 0.664499 4.3:-7293944 MS.gene000412:CDS
TGGTTAAAGGAAAACTATGG+TGG 0.666602 4.3:-7293924 MS.gene000412:CDS
GCAAGAAGGTGGTCTCGATG+TGG 0.670613 4.3:-7294000 MS.gene000412:CDS
TTGGTGATCAGAATAAGGGA+TGG 0.686077 4.3:+7294508 None:intergenic
GAAACTTTCAGATAGACCTG+AGG 0.686755 4.3:-7293204 MS.gene000412:CDS
GAGAGAGCAATTGAGCAATG+TGG 0.702693 4.3:-7293381 MS.gene000412:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTAATTTCATCAATTA+AGG + chr4.3:7293371-7293390 None:intergenic 15.0%
!!! ATTTCCATTATATAGTTTTT+AGG + chr4.3:7293281-7293300 None:intergenic 15.0%
!!! CATGTGTTAAATTTAATTTT+GGG - chr4.3:7293412-7293431 MS.gene000412:intron 15.0%
!!! GTTATTGATACAAATTATTT+TGG - chr4.3:7293902-7293921 MS.gene000412:intron 15.0%
!!! TGTTCTTTCATTTTATAATT+AGG - chr4.3:7293143-7293162 MS.gene000412:CDS 15.0%
!! AATAGATATACATATATAGG+TGG - chr4.3:7293437-7293456 MS.gene000412:intron 20.0%
!! ATAATGTATCATATATTTGC+AGG - chr4.3:7293817-7293836 MS.gene000412:intron 20.0%
!! CTATATAATTAAGCGTATAA+TGG - chr4.3:7293316-7293335 MS.gene000412:CDS 20.0%
!! GCTAATAGATATACATATAT+AGG - chr4.3:7293434-7293453 MS.gene000412:intron 20.0%
!! TAATGCTTATACAAGATTTA+TGG - chr4.3:7294372-7294391 MS.gene000412:intron 20.0%
!!! CGCTTAATTATATAGTTTTT+AGG + chr4.3:7293311-7293330 None:intergenic 20.0%
!!! GCATGTGTTAAATTTAATTT+TGG - chr4.3:7293411-7293430 MS.gene000412:intron 20.0%
!!! TTTATTAATTGTTATTGTCC+TGG - chr4.3:7294433-7294452 MS.gene000412:intron 20.0%
! ATGAATAATGTTTCAGAGAT+TGG - chr4.3:7294186-7294205 MS.gene000412:intron 25.0%
! GAAGCCTAAAAACTATATAA+TGG - chr4.3:7293274-7293293 MS.gene000412:CDS 25.0%
! GCAAGTTTATAAAGCAAATA+TGG - chr4.3:7293715-7293734 MS.gene000412:CDS 25.0%
! TAGAAATTAAATGCAACCTT+TGG + chr4.3:7293117-7293136 None:intergenic 25.0%
! TCTTATTAAACTATGTGATC+CGG + chr4.3:7293228-7293247 None:intergenic 25.0%
!! CAGGGATTAATTTCAATTTT+GGG - chr4.3:7293849-7293868 MS.gene000412:intron 25.0%
AATTCAGTTATCTTGCAACA+AGG + chr4.3:7293767-7293786 None:intergenic 30.0%
AATTGCTTGTTCAGGAATAA+GGG + chr4.3:7294536-7294555 None:intergenic 30.0%
AGATATACATATATAGGTGG+TGG - chr4.3:7293440-7293459 MS.gene000412:intron 30.0%
AGCTATGATTCCATTAATGA+AGG - chr4.3:7293943-7293962 MS.gene000412:CDS 30.0%
CTAAACTCGTGTATATAGTA+TGG + chr4.3:7294044-7294063 None:intergenic 30.0%
GGATCACATAGTTTAATAAG+AGG - chr4.3:7293227-7293246 MS.gene000412:CDS 30.0%
TAATTGCTTGTTCAGGAATA+AGG + chr4.3:7294537-7294556 None:intergenic 30.0%
TTATCTCATCCAAACACTAT+TGG + chr4.3:7293204-7293223 None:intergenic 30.0%
TTCAACTATCCAATAGTGTT+TGG - chr4.3:7293192-7293211 MS.gene000412:CDS 30.0%
TTCAGTTGAAAATGCTCATA+CGG - chr4.3:7293880-7293899 MS.gene000412:intron 30.0%
! ATAAAAATCTTTTCACCCTC+AGG + chr4.3:7294418-7294437 None:intergenic 30.0%
! GCAGGGATTAATTTCAATTT+TGG - chr4.3:7293848-7293867 MS.gene000412:intron 30.0%
! TAATTGTTATTGTCCTGGTT+GGG - chr4.3:7294438-7294457 MS.gene000412:intron 30.0%
! TGATTTTCTACATCAAGTAG+AGG - chr4.3:7294282-7294301 MS.gene000412:intron 30.0%
! TTAATTGTTATTGTCCTGGT+TGG - chr4.3:7294437-7294456 MS.gene000412:intron 30.0%
!! AAGAGGTGTTGAAATCAAAT+TGG - chr4.3:7293244-7293263 MS.gene000412:CDS 30.0%
AAACTTTCAGATAGACCTGA+GGG - chr4.3:7294400-7294419 MS.gene000412:intron 35.0%
ACAACAAGAGATGCTAATGT+TGG - chr4.3:7293559-7293578 MS.gene000412:intron 35.0%
AGTGTTTGGATGAGATAAAC+CGG - chr4.3:7293206-7293225 MS.gene000412:CDS 35.0%
ATTCCTGAACAAGCAATTAC+AGG - chr4.3:7294538-7294557 MS.gene000412:CDS 35.0%
ATTTGCAGGTAAACAATGCA+GGG - chr4.3:7293831-7293850 MS.gene000412:intron 35.0%
CAATGTGGATAATCTTTCAG+AGG - chr4.3:7294237-7294256 MS.gene000412:intron 35.0%
CATAGCTTCAATCATACGTT+TGG + chr4.3:7293930-7293949 None:intergenic 35.0%
CTATGGTGGCTTAAATATTC+TGG - chr4.3:7293693-7293712 MS.gene000412:CDS 35.0%
CTTTAACCACTCAGAGAATT+GGG + chr4.3:7293668-7293687 None:intergenic 35.0%
CTTTCAGAGGAACTTATTGA+TGG - chr4.3:7294250-7294269 MS.gene000412:intron 35.0%
GAGTGGTTAAAGGAAAACTA+TGG - chr4.3:7293676-7293695 MS.gene000412:CDS 35.0%
TATTTGCAGGTAAACAATGC+AGG - chr4.3:7293830-7293849 MS.gene000412:intron 35.0%
TCAATTAAGGTCTTGCAAGA+AGG - chr4.3:7293589-7293608 MS.gene000412:intron 35.0%
TGGTTAAAGGAAAACTATGG+TGG - chr4.3:7293679-7293698 MS.gene000412:CDS 35.0%
TTTCCTGTAATTGCTTGTTC+AGG + chr4.3:7294544-7294563 None:intergenic 35.0%
TTTCTACATCAAGTAGAGGA+TGG - chr4.3:7294286-7294305 MS.gene000412:intron 35.0%
! AACCTTTGGTGATCAGAATA+AGG + chr4.3:7293103-7293122 None:intergenic 35.0%
! ACCTTTGGTGATCAGAATAA+GGG + chr4.3:7293102-7293121 None:intergenic 35.0%
! TGTTGGCGTTGAATCAATTA+AGG - chr4.3:7293576-7293595 MS.gene000412:intron 35.0%
!! GATTGGTAATGATACGCTAA+CGG + chr4.3:7294100-7294119 None:intergenic 35.0%
ACTGGTGGAAATAGAGGAAT+TGG - chr4.3:7293487-7293506 MS.gene000412:intron 40.0%
AGAGGATGGAACTTGGAAAT+CGG - chr4.3:7294300-7294319 MS.gene000412:intron 40.0%
ATCAAGTAGAGGATGGAACT+TGG - chr4.3:7294293-7294312 MS.gene000412:intron 40.0%
ATTAAGGTCTTGCAAGAAGG+TGG - chr4.3:7293592-7293611 MS.gene000412:CDS 40.0%
CCAATTCTCTGAGTGGTTAA+AGG - chr4.3:7293666-7293685 MS.gene000412:CDS 40.0%
CCTTTAACCACTCAGAGAAT+TGG + chr4.3:7293669-7293688 None:intergenic 40.0%
CTGGTGGAAATAGAGGAATT+GGG - chr4.3:7293488-7293507 MS.gene000412:intron 40.0%
GAAACAATTGCTGTGGTTAC+TGG - chr4.3:7293469-7293488 MS.gene000412:intron 40.0%
GAAACTTTCAGATAGACCTG+AGG - chr4.3:7294399-7294418 MS.gene000412:intron 40.0%
GAGTAGTCGGTAAATATTCG+AGG + chr4.3:7294334-7294353 None:intergenic 40.0%
GTCTAAGGAAACAATTGCTG+TGG - chr4.3:7293462-7293481 MS.gene000412:intron 40.0%
GTTGCAGTTGCACATATGAT+TGG + chr4.3:7294117-7294136 None:intergenic 40.0%
TAGTTTCGACAACGAGTAGT+CGG + chr4.3:7294347-7294366 None:intergenic 40.0%
TCCCTTATTCTGATCACCAA+AGG - chr4.3:7293098-7293117 MS.gene000412:CDS 40.0%
! CATATATAGGTGGTGGTCTA+AGG - chr4.3:7293447-7293466 MS.gene000412:intron 40.0%
! TGGGTTAAAACAGCTCTAAC+AGG - chr4.3:7294457-7294476 MS.gene000412:intron 40.0%
! TTGGTGATCAGAATAAGGGA+TGG + chr4.3:7293098-7293117 None:intergenic 40.0%
!! CGCAAGAGATATCACTTTTG+AGG - chr4.3:7293071-7293090 MS.gene000412:CDS 40.0%
!! TCAAGACAACTTGCTGATCA+TGG - chr4.3:7293520-7293539 MS.gene000412:intron 40.0%
ACAATTGCTGTGGTTACTGG+TGG - chr4.3:7293472-7293491 MS.gene000412:intron 45.0%
GAGAGAGCAATTGAGCAATG+TGG - chr4.3:7294222-7294241 MS.gene000412:intron 45.0%
GAGGATGGAACTTGGAAATC+GGG - chr4.3:7294301-7294320 MS.gene000412:intron 45.0%
GTAAATGTTAGCTCGCGTCT+AGG - chr4.3:7293989-7294008 MS.gene000412:CDS 45.0%
GTGGTTACTGGTGGAAATAG+AGG - chr4.3:7293481-7293500 MS.gene000412:intron 45.0%
! ACAGCTCTAACAGGTTATGC+AGG - chr4.3:7294466-7294485 MS.gene000412:intron 45.0%
!!! AGAGCTGTTTTAACCCAACC+AGG + chr4.3:7294454-7294473 None:intergenic 45.0%
CCACTCAGAGAATTGGGTGA+TGG + chr4.3:7293662-7293681 None:intergenic 50.0%
CCATCACCCAATTCTCTGAG+TGG - chr4.3:7293659-7293678 MS.gene000412:CDS 50.0%
GAACTTGGAAATCGGGAGGA+TGG - chr4.3:7294308-7294327 MS.gene000412:intron 50.0%
GATGGAACTTGGAAATCGGG+AGG - chr4.3:7294304-7294323 MS.gene000412:intron 50.0%
GCAGCAGACGCCTTCATTAA+TGG + chr4.3:7293956-7293975 None:intergenic 50.0%
TCGCGTCTAGGTCGACTAAA+TGG - chr4.3:7294001-7294020 MS.gene000412:CDS 50.0%
!! ACGGTGCTGATACTGGAGTT+TGG - chr4.3:7294506-7294525 MS.gene000412:CDS 50.0%
!! GGAAGTGTCTCAGTTGAGGA+CGG - chr4.3:7294487-7294506 MS.gene000412:intron 50.0%
!! TGCAGGAAGTGTCTCAGTTG+AGG - chr4.3:7294483-7294502 MS.gene000412:intron 50.0%
!! TTAATGAAGGCGTCTGCTGC+TGG - chr4.3:7293956-7293975 MS.gene000412:CDS 50.0%
GCAAGAAGGTGGTCTCGATG+TGG - chr4.3:7293603-7293622 MS.gene000412:CDS 55.0%
!! GTTGAGGACGGTGCTGATAC+TGG - chr4.3:7294499-7294518 MS.gene000412:intron 55.0%
!! ATGAAGGCGTCTGCTGCTGG+TGG - chr4.3:7293959-7293978 MS.gene000412:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 7293028 7294597 7293028 ID=MS.gene000412
chr4.3 mRNA 7293028 7294597 7293028 ID=MS.gene000412.t1;Parent=MS.gene000412
chr4.3 exon 7294506 7294597 7294506 ID=MS.gene000412.t1.exon1;Parent=MS.gene000412.t1
chr4.3 CDS 7294506 7294597 7294506 ID=cds.MS.gene000412.t1;Parent=MS.gene000412.t1
chr4.3 exon 7293911 7294169 7293911 ID=MS.gene000412.t1.exon2;Parent=MS.gene000412.t1
chr4.3 CDS 7293911 7294169 7293911 ID=cds.MS.gene000412.t1;Parent=MS.gene000412.t1
chr4.3 exon 7293592 7293786 7293592 ID=MS.gene000412.t1.exon3;Parent=MS.gene000412.t1
chr4.3 CDS 7293592 7293786 7293592 ID=cds.MS.gene000412.t1;Parent=MS.gene000412.t1
chr4.3 exon 7293028 7293423 7293028 ID=MS.gene000412.t1.exon4;Parent=MS.gene000412.t1
chr4.3 CDS 7293028 7293423 7293028 ID=cds.MS.gene000412.t1;Parent=MS.gene000412.t1
Gene Sequence

>MS.gene000412

ATGGGGCACAAAGAAAAATCAAAAGAGAAAAAAGATAAGAGATCGCAAGAGATATCACTTTTGAGGACCATCCCTTATTCTGATCACCAAAGGTTGCATTTAATTTCTAGCTATATGTTCTTTCATTTTATAATTAGGTGTAATTTGTGTGAGTTTAAATATAATTCAACTATCCAATAGTGTTTGGATGAGATAAACCGGATCACATAGTTTAATAAGAGGTGTTGAAATCAAATTGGAAATGCAGAAGCCTAAAAACTATATAATGGAAATGCAGAAGCCTAAAAACTATATAATTAAGCGTATAATGGCATTGATTTTTGAATCAATAGTTAACAATCCTTAATTGATGAAATTAATGAATACTAGTATATTTCTAACTAGCATGTGTTAAATTTAATTTTGGGCTAATAGATATACATATATAGGTGGTGGTCTAAGGAAACAATTGCTGTGGTTACTGGTGGAAATAGAGGAATTGGGTTTGAAATTTCAAGACAACTTGCTGATCATGGAGTAACTGTAATATTAACAACAAGAGATGCTAATGTTGGCGTTGAATCAATTAAGGTCTTGCAAGAAGGTGGTCTCGATGTGGCATGTCATCAACTTGATGTATTAGATTCTTCATCCATCACCCAATTCTCTGAGTGGTTAAAGGAAAACTATGGTGGCTTAAATATTCTGGCAAGTTTATAAAGCAAATATGGTTAATTTCTAGCTTTCTTTCTTACAACCTTGTTGCAAGATAACTGAATTAAATTAAATGTCTGCGTATGTATCTAAACAATAATGTATCATATATTTGCAGGTAAACAATGCAGGGATTAATTTCAATTTTGGGTCTGATAATTCAGTTGAAAATGCTCATACGGTTATTGATACAAATTATTTTGGAACCAAACGTATGATTGAAGCTATGATTCCATTAATGAAGGCGTCTGCTGCTGGTGGTCGTATTGTAAATGTTAGCTCGCGTCTAGGTCGACTAAATGGAAAACGAAATGTAAGTACCATACTATATACACGAGTTTAGTTATGTCACACAGTCAATTTTAAAAGTTTTATACCGTTAGCGTATCATTACCAATCATATGTGCAACTGCAACTTTAGAATTAACTATGATTAAATTAAACATTTTTAATGATATGATAATTATGAATAATGTTTCAGAGATTGGAGAATGATGATTTGAGAGAGCAATTGAGCAATGTGGATAATCTTTCAGAGGAACTTATTGATGGTGTTGTGACTGATTTTCTACATCAAGTAGAGGATGGAACTTGGAAATCGGGAGGATGGCCTCGAATATTTACCGACTACTCGTTGTCGAAACTAGCTGTTAATGCTTATACAAGATTTATGGCAAAGAAACTTTCAGATAGACCTGAGGGTGAAAAGATTTTTATTAATTGTTATTGTCCTGGTTGGGTTAAAACAGCTCTAACAGGTTATGCAGGAAGTGTCTCAGTTGAGGACGGTGCTGATACTGGAGTTTGGCTTTCCCTTATTCCTGAACAAGCAATTACAGGAAAGTTTTTTGCTGAGAGAAGAGAAATCAACTTCTAA
Protein sequence

>MS.gene000412.t1

MGHKEKSKEKKDKRSQEISLLRTIPYSDHQRWWSKETIAVVTGGNRGIGFEISRQLADHGVTVILTTRDANVGVESIKVLQEGGLDVACHQLDVLDSSSITQFSEWLKENYGGLNILVNNAGINFNFGSDNSVENAHTVIDTNYFGTKRMIEAMIPLMKASAAGGRIVNVSSRLGRLNGKRNRLENDDLREQLSNVDNLSEELIDGVVTDFLHQVEDGTWKSGGWPRIFTDYSLSKLAVNAYTRFMAKKLSDRPEGEKIFINCYCPGWVKTALTGYAGSVSVEDGADTGVWLSLIPEQAITGKFFAERREINF