AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009163

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009163.t1 MTR_8g032000 89.015 264 28 1 1 263 1 264 1.70e-173 483
MS.gene009163.t1 MTR_8g031990 87.452 263 32 1 1 262 1 263 2.93e-167 468
MS.gene009163.t1 MTR_8g032010 85.171 263 38 1 1 262 1 263 3.83e-162 455
MS.gene009163.t1 MTR_8g032020 83.333 264 42 2 2 263 3 266 1.15e-156 441
MS.gene009163.t1 MTR_8g031980 70.455 264 77 1 1 263 1 264 4.46e-136 389
MS.gene009163.t1 MTR_8g031960 70.656 259 73 2 7 263 8 265 1.47e-133 382
MS.gene009163.t1 MTR_8g031940 67.925 265 83 2 1 263 1 265 2.74e-128 369
MS.gene009163.t1 MTR_7g076660 57.414 263 101 2 1 263 1 252 6.00e-106 311
MS.gene009163.t1 MTR_7g076655 57.414 263 101 2 1 263 1 252 3.47e-105 310
MS.gene009163.t1 MTR_7g076665 58.555 263 105 2 1 262 1 260 5.65e-105 310
MS.gene009163.t1 MTR_8g031900 54.643 280 119 4 1 279 1 273 2.80e-104 308
MS.gene009163.t1 MTR_7g076660 57.588 257 98 2 7 263 8 253 3.28e-104 307
MS.gene009163.t1 MTR_8g031910 57.795 263 99 3 1 262 1 252 6.52e-104 306
MS.gene009163.t1 MTR_8g031630 57.197 264 110 3 1 262 1 263 2.42e-100 298
MS.gene009163.t1 MTR_8g031600 56.391 266 109 3 1 262 1 263 2.18e-97 290
MS.gene009163.t1 MTR_8g031650 57.197 264 110 3 1 262 1 263 5.58e-97 298
MS.gene009163.t1 MTR_8g031650 56.471 255 108 3 10 262 270 523 2.00e-91 284
MS.gene009163.t1 MTR_8g031610 56.031 257 109 3 8 262 6 260 5.37e-95 284
MS.gene009163.t1 MTR_8g032030 81.609 174 30 1 1 172 1 174 2.52e-93 278
MS.gene009163.t1 MTR_5g062280 52.290 262 124 1 1 262 1 261 3.54e-93 280
MS.gene009163.t1 MTR_8g031650 56.471 255 108 3 10 262 104 357 4.74e-93 283
MS.gene009163.t1 MTR_8g031650 57.292 96 39 2 169 262 2 97 6.75e-32 123
MS.gene009163.t1 MTR_7g076660 56.303 238 93 2 26 263 2 228 7.02e-93 278
MS.gene009163.t1 MTR_3g079320 49.242 264 125 4 6 262 10 271 3.41e-81 249
MS.gene009163.t1 MTR_8g031850 45.357 280 107 5 1 279 1 235 2.16e-75 233
MS.gene009163.t1 MTR_7g106800 52.402 229 76 4 7 230 9 209 5.46e-69 217
MS.gene009163.t1 MTR_3g098470 41.762 261 116 5 9 258 37 272 3.25e-58 191
MS.gene009163.t1 MTR_5g083430 59.854 137 55 0 126 262 13 149 9.80e-58 185
MS.gene009163.t1 MTR_1g031180 36.630 273 134 6 9 267 42 289 5.09e-50 169
MS.gene009163.t1 MTR_4g087460 37.037 270 131 7 9 263 24 269 1.16e-47 163
MS.gene009163.t1 MTR_8g096060 37.165 261 125 5 9 258 39 271 6.15e-47 161
MS.gene009163.t1 MTR_8g063590 95.714 70 3 0 261 330 419 488 7.73e-30 119
MS.gene009163.t1 MTR_5g090290 72.857 70 19 0 261 330 411 480 5.27e-28 114
MS.gene009163.t1 MTR_2g022980 55.682 88 34 1 248 330 395 482 3.05e-23 100
MS.gene009163.t1 MTR_5g087930 69.841 63 19 0 261 323 370 432 6.75e-23 99.4
MS.gene009163.t1 MTR_5g033100 56.338 71 31 0 261 331 420 490 1.52e-21 95.9
MS.gene009163.t1 MTR_7g087430 54.795 73 33 0 261 333 306 378 1.39e-20 92.0
MS.gene009163.t1 MTR_2g088400 55.714 70 31 0 261 330 417 486 7.65e-20 90.5
MS.gene009163.t1 MTR_7g087400 54.286 70 32 0 261 330 393 462 2.45e-19 89.0
MS.gene009163.t1 MTR_1g021740 37.952 166 78 5 173 331 322 469 2.48e-19 89.0
MS.gene009163.t1 MTR_7g087370 51.429 70 34 0 261 330 364 433 2.71e-19 89.0
MS.gene009163.t1 MTR_4g048000 51.899 79 38 0 261 339 429 507 2.71e-19 89.0
MS.gene009163.t1 MTR_8g069470 51.899 79 38 0 261 339 414 492 2.79e-19 89.0
MS.gene009163.t1 MTR_6g047640 57.143 70 30 0 261 330 375 444 5.96e-19 87.8
MS.gene009163.t1 MTR_1g100180 57.534 73 31 0 261 333 406 478 6.95e-19 87.8
MS.gene009163.t1 MTR_6g047640 57.143 70 30 0 261 330 394 463 8.20e-19 87.4
MS.gene009163.t1 MTR_3g099700 56.944 72 30 1 261 331 410 481 1.90e-18 86.7
MS.gene009163.t1 MTR_5g032720 55.556 72 31 1 261 331 400 471 2.55e-18 85.9
MS.gene009163.t1 MTR_6g047610 54.286 70 32 0 261 330 393 462 3.53e-18 85.5
MS.gene009163.t1 MTR_2g079030 55.072 69 31 0 261 329 445 513 3.69e-18 85.9
MS.gene009163.t1 MTR_7g087410 50.000 70 35 0 261 330 306 375 4.65e-18 84.7
MS.gene009163.t1 MTR_5g032750 55.556 72 31 1 261 331 400 471 5.72e-18 85.1
MS.gene009163.t1 MTR_7g087410 50.000 70 35 0 261 330 395 464 5.77e-18 85.1
MS.gene009163.t1 MTR_3g099720 43.956 91 43 1 245 335 397 479 2.42e-17 83.2
MS.gene009163.t1 MTR_2g078960 51.316 76 37 0 261 336 398 473 4.32e-17 82.4
MS.gene009163.t1 MTR_2g079020 51.316 76 37 0 261 336 402 477 9.22e-17 81.3
MS.gene009163.t1 MTR_6g081780 49.296 71 36 0 261 331 392 462 1.56e-16 80.9
MS.gene009163.t1 MTR_7g082810 51.389 72 35 0 261 332 385 456 1.58e-16 80.5
MS.gene009163.t1 MTR_6g081410 47.887 71 37 0 261 331 392 462 2.32e-16 80.1
MS.gene009163.t1 MTR_5g090280 50.725 69 34 0 262 330 328 396 3.87e-16 79.3
MS.gene009163.t1 MTR_6g081810 50.704 71 35 0 261 331 366 436 5.06e-16 79.0
MS.gene009163.t1 MTR_2g078990 47.368 76 40 0 261 336 225 300 8.26e-16 77.4
MS.gene009163.t1 MTR_1g109060 49.315 73 37 0 261 333 389 461 2.27e-15 77.0
MS.gene009163.t1 MTR_2g078930 48.684 76 39 0 261 336 408 483 2.75e-15 77.0
MS.gene009163.t1 MTR_6g004220 49.296 71 36 0 261 331 389 459 4.62e-15 76.3
MS.gene009163.t1 MTR_2g078970 46.053 76 41 0 261 336 396 471 7.12e-15 75.9
MS.gene009163.t1 MTR_6g081300 29.442 197 105 5 151 331 267 445 1.16e-14 75.1
MS.gene009163.t1 MTR_7g082800 52.113 71 34 0 261 331 307 377 1.23e-14 74.7
MS.gene009163.t1 MTR_6g004220 48.571 70 36 0 261 330 396 465 1.38e-14 74.7
MS.gene009163.t1 MTR_1g108990 47.945 73 38 0 261 333 390 462 1.77e-14 74.3
MS.gene009163.t1 MTR_1g109060 50.000 70 35 0 261 330 389 458 1.98e-14 74.3
MS.gene009163.t1 MTR_8g106670 43.662 71 40 0 261 331 377 447 4.73e-14 73.2
MS.gene009163.t1 MTR_6g027190 51.429 70 34 0 261 330 388 457 4.98e-14 73.2
MS.gene009163.t1 MTR_6g081310 30.412 194 107 4 151 331 265 443 1.89e-13 71.2
MS.gene009163.t1 MTR_6g081390 45.714 70 38 0 262 331 379 448 2.65e-13 70.9
MS.gene009163.t1 MTR_6g027200 48.571 70 36 0 261 330 400 469 7.60e-13 69.7
MS.gene009163.t1 MTR_6g081240 36.585 82 52 0 252 333 366 447 2.09e-12 68.2
MS.gene009163.t1 MTR_6g066380 45.070 71 39 0 261 331 394 464 2.93e-12 67.8
MS.gene009163.t1 MTR_1g108810 41.429 70 41 0 261 330 399 468 4.39e-12 67.4
MS.gene009163.t1 MTR_6g047590 52.857 70 33 0 261 330 393 462 1.15e-11 65.9
MS.gene009163.t1 MTR_6g081400 42.857 70 40 0 262 331 382 451 1.26e-11 65.9
MS.gene009163.t1 MTR_1g108840 41.429 70 41 0 262 331 407 476 3.01e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009163.t1 AT3G61220 55.892 297 123 5 1 295 1 291 3.46e-108 318
MS.gene009163.t1 AT3G61220 56.014 291 120 5 7 295 14 298 1.14e-105 311
MS.gene009163.t1 AT3G61220 56.014 291 120 5 7 295 38 322 3.20e-105 311
MS.gene009163.t1 AT2G24190 53.535 297 130 5 1 295 1 291 2.39e-101 300
MS.gene009163.t1 AT2G24190 53.952 291 126 5 7 295 12 296 1.56e-99 296
MS.gene009163.t1 AT1G01800 53.585 265 117 5 1 263 1 261 4.55e-96 287
MS.gene009163.t1 AT1G01800 48.851 174 87 2 90 263 55 226 2.28e-56 184
MS.gene009163.t1 AT5G51030 37.358 265 130 5 9 262 40 279 1.83e-53 178
MS.gene009163.t1 AT5G61830 39.080 261 125 4 9 258 39 276 2.51e-48 165
MS.gene009163.t1 AT5G61830 39.080 261 125 4 9 258 39 276 2.51e-48 165
MS.gene009163.t1 AT3G59710 35.662 272 135 7 4 263 10 253 2.74e-46 159
MS.gene009163.t1 AT3G59710 36.154 260 132 6 9 262 31 262 1.74e-45 157
MS.gene009163.t1 AT3G59710 36.015 261 133 6 9 263 50 282 3.73e-45 157
MS.gene009163.t1 AT3G59030 73.973 73 19 0 261 333 415 487 2.17e-30 121
MS.gene009163.t1 AT1G61890 59.459 74 29 1 261 334 411 483 9.55e-23 99.4
MS.gene009163.t1 AT1G61890 60.000 70 28 0 261 330 411 480 1.04e-22 99.0
MS.gene009163.t1 AT1G11670 61.429 70 27 0 261 330 414 483 2.44e-22 98.2
MS.gene009163.t1 AT4G21910 52.273 88 37 1 248 330 400 487 3.23e-22 97.8
MS.gene009163.t1 AT4G21910 52.273 88 37 1 248 330 400 487 3.23e-22 97.8
MS.gene009163.t1 AT4G21910 52.273 88 37 1 248 330 402 489 3.89e-22 97.4
MS.gene009163.t1 AT4G21910 52.273 88 37 1 248 330 402 489 3.89e-22 97.4
MS.gene009163.t1 AT1G33100 61.429 70 27 0 261 330 396 465 5.49e-22 97.1
MS.gene009163.t1 AT4G21903 49.462 93 42 1 248 335 398 490 5.79e-22 97.1
MS.gene009163.t1 AT4G21903 51.136 88 38 1 248 330 289 376 9.04e-22 95.5
MS.gene009163.t1 AT5G44050 38.547 179 88 7 161 333 314 476 1.10e-21 95.9
MS.gene009163.t1 AT4G21903 51.136 88 38 1 248 330 398 485 1.20e-21 95.9
MS.gene009163.t1 AT1G47530 63.380 71 26 0 261 331 306 376 1.62e-21 94.7
MS.gene009163.t1 AT1G47530 63.380 71 26 0 261 331 398 468 1.74e-21 95.5
MS.gene009163.t1 AT1G33110 58.571 70 29 0 261 330 399 468 3.20e-21 94.7
MS.gene009163.t1 AT1G12950 53.521 71 33 0 261 331 430 500 4.85e-21 94.4
MS.gene009163.t1 AT1G33090 55.714 70 31 0 261 330 399 468 5.38e-21 94.0
MS.gene009163.t1 AT1G23300 43.243 111 49 2 235 331 367 477 1.67e-20 92.8
MS.gene009163.t1 AT1G33080 57.143 70 30 0 261 330 399 468 1.98e-19 89.4
MS.gene009163.t1 AT1G33080 57.143 70 30 0 261 330 399 468 2.55e-19 89.0
MS.gene009163.t1 AT4G00350 60.000 70 28 0 261 330 450 519 2.90e-19 89.0
MS.gene009163.t1 AT5G38030 49.333 75 38 0 261 335 408 482 1.51e-18 86.7
MS.gene009163.t1 AT3G26590 50.704 71 35 0 261 331 408 478 1.74e-18 86.7
MS.gene009163.t1 AT3G26590 50.704 71 35 0 261 331 306 376 1.79e-18 85.9
MS.gene009163.t1 AT5G65380 54.167 72 32 1 261 331 400 471 1.12e-17 84.3
MS.gene009163.t1 AT3G21690 45.455 88 43 1 248 330 398 485 1.32e-17 84.0
MS.gene009163.t1 AT1G66760 34.959 123 69 3 210 332 352 463 1.21e-15 78.2
MS.gene009163.t1 AT3G03620 50.000 70 35 0 261 330 400 469 1.37e-15 77.8
MS.gene009163.t1 AT3G03620 50.000 70 35 0 261 330 400 469 1.37e-15 77.8
MS.gene009163.t1 AT1G66760 34.146 123 70 3 210 332 352 463 5.14e-15 76.3
MS.gene009163.t1 AT1G66760 34.146 123 70 3 210 332 224 335 6.85e-15 75.1
MS.gene009163.t1 AT2G34360 47.143 70 37 0 261 330 343 412 8.51e-15 75.5
MS.gene009163.t1 AT2G34360 47.143 70 37 0 261 330 385 454 1.08e-14 75.1
MS.gene009163.t1 AT1G66780 47.143 70 37 0 261 330 399 468 1.27e-14 75.1
MS.gene009163.t1 AT5G17700 48.571 70 36 0 261 330 397 466 1.33e-14 75.1
MS.gene009163.t1 AT5G17700 48.571 70 36 0 261 330 397 466 1.33e-14 75.1
MS.gene009163.t1 AT5G10420 58.333 72 29 1 261 331 401 472 1.96e-13 71.2
MS.gene009163.t1 AT2G04066 42.254 71 41 0 261 331 86 156 4.24e-13 67.0
MS.gene009163.t1 AT3G23560 46.377 69 37 0 261 329 403 471 4.28e-13 70.5
MS.gene009163.t1 AT2G04070 38.776 98 56 1 241 338 375 468 4.47e-13 70.5
MS.gene009163.t1 AT5G52450 46.479 71 38 0 261 331 390 460 5.61e-13 70.1
MS.gene009163.t1 AT2G04050 38.542 96 55 1 236 331 370 461 9.01e-13 69.3
MS.gene009163.t1 AT2G04100 27.444 266 134 13 77 331 247 464 1.15e-12 68.9
MS.gene009163.t1 AT3G23550 43.478 69 39 0 261 329 395 463 1.84e-12 68.6
MS.gene009163.t1 AT2G04040 38.462 91 52 1 241 331 375 461 2.15e-12 68.2
MS.gene009163.t1 AT1G15150 45.070 71 39 0 261 331 395 465 2.91e-12 67.8
MS.gene009163.t1 AT1G15160 45.070 71 39 0 261 331 395 465 3.16e-12 67.8
MS.gene009163.t1 AT1G15150 45.070 71 39 0 261 331 279 349 3.26e-12 67.4
MS.gene009163.t1 AT2G04100 27.106 273 139 14 77 337 247 471 3.75e-12 67.4
MS.gene009163.t1 AT1G71140 47.887 71 37 0 261 331 390 460 3.77e-12 67.4
MS.gene009163.t1 AT2G04090 45.070 71 39 0 261 331 394 464 5.96e-12 67.0
MS.gene009163.t1 AT1G15170 45.070 71 39 0 261 331 398 468 6.75e-12 66.6
MS.gene009163.t1 AT1G64820 35.165 91 55 1 241 331 377 463 6.98e-12 66.6
MS.gene009163.t1 AT1G15170 45.070 71 39 0 261 331 279 349 7.45e-12 66.2
MS.gene009163.t1 AT2G04080 42.254 71 41 0 261 331 391 461 2.98e-11 64.7
MS.gene009163.t1 AT4G25640 53.226 62 29 0 269 330 404 465 8.74e-11 63.5
MS.gene009163.t1 AT4G25640 53.226 62 29 0 269 330 404 465 9.92e-11 63.2

Find 71 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATATCAAGTTTGCCAAAT+TGG 0.158459 8.1:-40068555 None:intergenic
ATTTGGCAAACTTGATATTC+TGG 0.220906 8.1:+40068558 MS.gene009163:CDS
TGCATTCAGATAATTTATTA+TGG 0.275367 8.1:+40073746 MS.gene009163:CDS
CTCCCCATTGGTTGTGTCCT+TGG 0.278608 8.1:+40075633 MS.gene009163:CDS
ACACAATTGATGCAGAAATT+AGG 0.291057 8.1:-40074439 None:intergenic
TAGAATAGATGAAGTGATAA+AGG 0.310520 8.1:+40074294 MS.gene009163:CDS
TTGGCTGCTTATTATCTTGT+TGG 0.320115 8.1:+40075609 MS.gene009163:CDS
GTTAACAATGCGGGGATTAC+CGG 0.349470 8.1:+40072763 MS.gene009163:CDS
AATATCAAGTTTGCCAAATT+GGG 0.350754 8.1:-40068554 None:intergenic
TGGAAGTGGATGGCAGGATT+TGG 0.362780 8.1:+40075572 MS.gene009163:CDS
GACATTCTAGGTGTGGCTAT+TGG 0.373130 8.1:+40075552 MS.gene009163:intron
CTTTGCAAGAATTCTTGTAT+AGG 0.377721 8.1:-40074410 None:intergenic
GACTCCTTTAGCCCATTCAT+TGG 0.379973 8.1:-40074242 None:intergenic
AGATGGGAGAACCGACAAAA+AGG 0.380595 8.1:+40068225 None:intergenic
TTGGAAACACTTAAAGCATC+TGG 0.383030 8.1:+40068451 MS.gene009163:CDS
GACAAGAGGTCTTCATGCTT+TGG 0.386212 8.1:+40068432 MS.gene009163:CDS
GCCCTTCTCAATTCTTCATC+AGG 0.392272 8.1:-40073692 None:intergenic
ACAGGTGCAAACAAAGGAAT+TGG 0.392287 8.1:+40068346 MS.gene009163:CDS
ACTGATATGACTAATAATAA+TGG 0.402223 8.1:+40074481 MS.gene009163:CDS
GAATTGGATTTGAGATAGTA+AGG 0.414626 8.1:+40068362 MS.gene009163:CDS
ATGTGGTGGGGCATGATTGT+TGG 0.415222 8.1:+40075793 MS.gene009163:CDS
CTCAGGTATGCGGTGGTGAC+AGG 0.424710 8.1:+40068328 MS.gene009163:intron
TATCTTGTTGGTCTCCCCAT+TGG 0.426203 8.1:+40075621 MS.gene009163:CDS
GAGAAGCTACTAAGTGGATA+AGG 0.433040 8.1:+40076130 MS.gene009163:CDS
CTCAATTCTTCATCAGGTAG+TGG 0.444553 8.1:-40073686 None:intergenic
ATAATTGAAGGAGGGGAAGA+AGG 0.467120 8.1:-40073790 None:intergenic
TTCTTACAATAAGAACAGAT+TGG 0.476511 8.1:+40075845 MS.gene009163:CDS
ACCAGCGTGTATCCAATGAA+TGG 0.476991 8.1:+40074230 MS.gene009163:intron
GATGATACATTGACTACTCT+TGG 0.477012 8.1:-40073821 None:intergenic
TTTGGTAGCTTATGTTAACT+TGG 0.477458 8.1:+40075590 MS.gene009163:CDS
ATTATGTAGGTTAACAATGC+GGG 0.478165 8.1:+40072754 MS.gene009163:intron
TCTTACAATAAGAACAGATT+GGG 0.490816 8.1:+40075846 MS.gene009163:CDS
TTGGTGAATCAGATAATTGA+AGG 0.495258 8.1:-40073802 None:intergenic
AAACCAAGGACACAACCAAT+GGG 0.495877 8.1:-40075636 None:intergenic
TAGAAAGAAAAGGTTGGCCT+AGG 0.496483 8.1:+40074347 MS.gene009163:CDS
ACTAACCCCACTCGTAATGA+TGG 0.500457 8.1:-40072818 None:intergenic
GTAAGGCAGTTAGCTTCAGC+TGG 0.501587 8.1:+40068379 MS.gene009163:CDS
TGCATCAATTGTGTTTGCCC+TGG 0.503943 8.1:+40074448 MS.gene009163:CDS
CCAGCGTGTATCCAATGAAT+GGG 0.516012 8.1:+40074231 MS.gene009163:intron
GAAGGGTCCTTAGAAAGAAA+AGG 0.520604 8.1:+40074337 MS.gene009163:CDS
ACGGTCTTTAATTTCAACTC+CGG 0.530691 8.1:-40072782 None:intergenic
GGCTATTGGAAGTGGATGGC+AGG 0.546538 8.1:+40075566 MS.gene009163:CDS
ATATCAGTCTTAACATAACC+AGG 0.548337 8.1:-40074466 None:intergenic
GAATCAGATAATTGAAGGAG+GGG 0.548419 8.1:-40073797 None:intergenic
CTAGGTGTGGCTATTGGAAG+TGG 0.558157 8.1:+40075558 MS.gene009163:intron
TAACTTCCATCATTACGAGT+GGG 0.560920 8.1:+40072812 MS.gene009163:CDS
AAAACCAAGGACACAACCAA+TGG 0.561033 8.1:-40075637 None:intergenic
TGAATCAGATAATTGAAGGA+GGG 0.564567 8.1:-40073798 None:intergenic
GTATCCAATGAATGGGCTAA+AGG 0.566596 8.1:+40074238 MS.gene009163:CDS
GTGTAGGCAGCGGCATACCT+AGG 0.573357 8.1:-40074364 None:intergenic
AGAAGCTTTAGCAACAGTGT+AGG 0.581837 8.1:-40074380 None:intergenic
TGATGGAAGTTAATAAATCA+CGG 0.586049 8.1:-40072801 None:intergenic
AACTTCCATCATTACGAGTG+GGG 0.593279 8.1:+40072813 MS.gene009163:CDS
AAGAACAGATTGGGACAAAG+AGG 0.594637 8.1:+40075855 MS.gene009163:CDS
ATCTCTCTTTCTCAGGTATG+CGG 0.601211 8.1:+40068318 MS.gene009163:intron
TACCTGATGAAGAATTGAGA+AGG 0.607021 8.1:+40073690 MS.gene009163:CDS
TTATGTAGGTTAACAATGCG+GGG 0.609230 8.1:+40072755 MS.gene009163:intron
CATCTATTCTATCTTCTGTG+AGG 0.618244 8.1:-40074282 None:intergenic
GTGAATCAGATAATTGAAGG+AGG 0.618406 8.1:-40073799 None:intergenic
TTTAGCAACAGTGTAGGCAG+CGG 0.618769 8.1:-40074374 None:intergenic
TATTATGTAGGTTAACAATG+CGG 0.621234 8.1:+40072753 MS.gene009163:intron
ACCTGATGAAGAATTGAGAA+GGG 0.623366 8.1:+40073691 MS.gene009163:CDS
TTAACTTCCATCATTACGAG+TGG 0.626372 8.1:+40072811 MS.gene009163:CDS
GTGTGGCTATTGGAAGTGGA+TGG 0.633567 8.1:+40075562 MS.gene009163:CDS
GGTCCTTAGAAAGAAAAGGT+TGG 0.636847 8.1:+40074341 MS.gene009163:CDS
TCTCTTTCTCAGGTATGCGG+TGG 0.642403 8.1:+40068321 MS.gene009163:intron
ACAGCAAGAGATGAGACAAG+AGG 0.648018 8.1:+40068418 MS.gene009163:CDS
AGCTTCAGCTGGAATCAAAG+TGG 0.664366 8.1:+40068390 MS.gene009163:CDS
GTGGTGACAGGTGCAAACAA+AGG 0.667851 8.1:+40068340 MS.gene009163:CDS
TATCAGTCTTAACATAACCA+GGG 0.713837 8.1:-40074465 None:intergenic
AACCAAGGACACAACCAATG+GGG 0.726331 8.1:-40075635 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.1 gene 40068227 40076150 40068227 ID=MS.gene009163
chr8.1 mRNA 40068227 40076150 40068227 ID=MS.gene009163.t1;Parent=MS.gene009163
chr8.1 exon 40068227 40068246 40068227 ID=MS.gene009163.t1.exon1;Parent=MS.gene009163.t1
chr8.1 CDS 40068227 40068246 40068227 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40068333 40068579 40068333 ID=MS.gene009163.t1.exon2;Parent=MS.gene009163.t1
chr8.1 CDS 40068333 40068579 40068333 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40072763 40072834 40072763 ID=MS.gene009163.t1.exon3;Parent=MS.gene009163.t1
chr8.1 CDS 40072763 40072834 40072763 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40073686 40073862 40073686 ID=MS.gene009163.t1.exon4;Parent=MS.gene009163.t1
chr8.1 CDS 40073686 40073862 40073686 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40074235 40074502 40074235 ID=MS.gene009163.t1.exon5;Parent=MS.gene009163.t1
chr8.1 CDS 40074235 40074502 40074235 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40075562 40075680 40075562 ID=MS.gene009163.t1.exon6;Parent=MS.gene009163.t1
chr8.1 CDS 40075562 40075680 40075562 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40075790 40075876 40075790 ID=MS.gene009163.t1.exon7;Parent=MS.gene009163.t1
chr8.1 CDS 40075790 40075876 40075790 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
chr8.1 exon 40076118 40076150 40076118 ID=MS.gene009163.t1.exon8;Parent=MS.gene009163.t1
chr8.1 CDS 40076118 40076150 40076118 ID=cds.MS.gene009163.t1;Parent=MS.gene009163.t1
Gene Sequence

>MS.gene009163

ATGGGAGAACCGACAAAAAGGTAGTATCAAACTTACTTTATCATTTCCAAAACATGTTTTTGAATATTATATAGATCTTCATTATTCTTGTATCTCTCTTTCTCAGGTATGCGGTGGTGACAGGTGCAAACAAAGGAATTGGATTTGAGATAGTAAGGCAGTTAGCTTCAGCTGGAATCAAAGTGGTGCTTACAGCAAGAGATGAGACAAGAGGTCTTCATGCTTTGGAAACACTTAAAGCATCTGGTTTATCTGATTTTGTTGTATTTCATCAACTAGATGTTGCTGATGCTTCAAGTGTAGCTTCTCTAGCAGATTTTGTCAAATCCCAATTTGGCAAACTTGATATTCTGGTAACGTGTTAAATATGTTTTAGTGAAAAAAACAATCTTTTGATTTTAGTCCATCTAAAAGTTTTTACTGATATGATACTTAAAATAAGGAGTGATTATGGATTATTATTAGGGATTGTTAGTTTTAGAATAAATTAATATGACATAGGTTTAGGAGATTAGTTTAATTCTGCTCTATCTTTTAGTAAAAGGTTATCTAGGAATTCTAGTATAATTGCTATTTTGTAGTTGTTATAGCTATGAGTATGCATCAGCTTATATAAGCATCGTATCACTCATGATTAGAGTAAATGAAAAATAATCAAGTTTGCTTTCTAAATTCTCTCTTAAATCCTGGAGTGTGATCCGTACTCGAAAAGTATAATTGGGTCGTATCATTATCATTTGGTGCTTTCATTCTTGATCTCATAATCCCCCTAATTAAGATCAATTATGGAGATGCCTATTGGTGGATCCTTTGTATTTCAAGGCAACCGGAAAATCTGAGATTGCAAAGATGATGGTGGCTGCACTGGCCATGCGCGGTGGTGCTCTCAAATGGTGGCTATGGTGGTCTCGCCATCACCCACAGTCGGATTGGGACACTTTTACCACTGGCGTTTCAAGCCTGAATGGCGGCATCTTTTGCCCGTATTAGAGATGGAAACGAAGGAAGCTTGTGAATCATCAACGTAGGTAAGGCTTTCCCGAGTCGAAGATTGTGTTGTTGATCTCACTCTCCAGCATTGGCACTTCAACCACCACACATAGTTTTTCTATTCGACCCTGGGGGGGTCTCAATTTCTGAAAACATCGCACTTTAAATTCTACAAGGATGTTGTCACGAATGTAGTTCCATTGCAATGGTGGTGGTACAACTGCCCAGCCACAATCATTTCGTCCGAGATTCCCTCACCGGAACCACACGACAGTGCTGCAATTCCACCGCCACTGGCCACCACCTTAAACAACTCTCTTACATTGGTTTCTTGTAGTTGTACTGATGTGTTACCTAGATTTTGTCGTGGTGAATTCTCTGGTTTTGAAAATAATGAATCAAATGAATATGTGAAAATTGAGTTCTCACACAACACCTGCTACATGTGTGCTATAATGCCAATCAGACCGTATTTCTTTTGGAAAGCCAACTTCTTTCAGGTCTATGCTTAGGAGGAGTACATCTTTAAAGTCATATATGTTCTTCTTGGACAACAATTTACCAGCTGATAGTGATTTTAATCAATTGAACCTTGAGCTTAATTCTCTTGCTGCTGTAGGCTTGTCCACATGTCCATATGATCCATATATTGATAATACTTGTGGTAGTAGTTGTGTTTATCCAAATCTTCATTTACGTTGTAATGATTTTGGAGGTATGTGTAATCCACATCATGCTTACAATTTTGAGGAAGATGTCATTGTCGTTGCTATTGACTTTGGTAACTCGCCCAAGGGTGATAACTTTAGAGATGATCTAGCTGCACTTATGTCCAAGCAAACTTTTATGGTTGAGCACATTGGAATACATATGTCAACTCATAAGAACCTGAGGCATGCTTCACTTGCATGGAAATTGAGGAGTGATTTCGGACATGTTATTGTCGGTAGTTGTATCACTATCCTTGGATATTCTTCTGGCCCCATGTATGAGAACAACTTCATGAGAGTTGCACAATTTTGGAGAGAGGATGATGATCTCTTTATTGGCTTTCACGATCAAATGCTGGTGTCATTTGTATAACTGCGCCGCTCACCGGAATCACGAACAACTCTTTCTATATGAATATAGTAGAAATGAGACAATTGATGAGTTCTTTCACGTGCAATTCTCAACAGCTACCACCCTTACTTGCATATTAGAATGCATGTTGAAATCTCCAATGTGTTGGTTGCTCCTTATTTTGCTGCCAGCATCTATCTCTTTGGTTCTAGCTTTGGAGTTGTATTGCTTGCTCTAACCTTCCCCATTCCTACTGATGTTATCTCTGAGTGTGGGCAGTCAATGCGGTTTTTTCACAGGGTCAATACATATCTTTTCAACTCATTGATCATAAATACCTCGGAAAATACCATAAAAGTTTTGAAGGCTTGGCTTCACAACCTTGCAAGCACAATGGTTGAAGCGGTTGGATCTACAACTATTATTGAAGCTATTGACCATGATCGTTTCAATATTTTCTCTATTCATCATAATTGCTTTAATATCGGGTCACAACCGAAAAACTTGAGGAATGTTGAGGGAGTTAATGGTGACATTGAGGAAGAATTGATTGAAATGGTGCATAAGCGATCTGTATTGTTTTTTATAATTGTAAAAAAGCATCAATGGGATCCTGGTGGCCTTTTGGATTTGGATATGCTTCCTATAGTGAGGTTCTCCCAATTCATGCAATGGGATCCAGGGAAGTTTAGTATAGAGGCCAAATTTTACAACCTTATGGATAAGGTTGATCTTGAAGGGGTCGGTAATGATATGATACTTAAAATAAGGAGTGATTATGGATTATTATTAGGGATTGTTAGTTTTAGAATAAATTAATATGACATAAGTTTAGGAGATTAGTTTAATTCTGCTCTATCTTATAGTAACAGGTTATTTAGGAATTCTAGTATAATTGCTATTTTGTAGTTGTTATAGTTATGAGTATGCATCAGCTTATATAAGCATCGTACCACTCATGATTAGAATAAATGAAAAATAATCAAGTTTGCTTTCTAAGTTCTGGAGTGTGATCCCCACTCGAATTGGGTCGTATCATTAACCCTTTTTAGTCTTAACATTGGTTTGATCCAATGATGAAAAAAACATGTATGCATGCAGGCATGGTGCAAGATCATTTTGTGCATGTTAGACAAAGACCTTAACACTATACATAAGTGTGCTAATAGTTTTACAGAAGAACTATGAAGTATCTTATTTTTATAAATTTTGAGGATTAAAAATTGATTTTTTTTTTTTTTTTGGAGACATTTATAAGATGAATAGACATACTTAACCCATTAATGAATATGTACAGTAGTAACTGAAGAAATATATAGCACATATCTTGAAATTTCTACACTTTAGACTAAAGTGAAACACTAATTCACTTGATATTATATTACAAAAACACATACAGCTACATTAACTTTTCCATTTGCATGATTTAAAATATTGTTCTATAGTTTTTTCGATCTTCTTTAGGTTCCGTTGTAAGTCAGGGAGTTCATCCTGCTATTCCAGTTTTTTATTTATTGTAATTCATTTATGCAAGTCATTTCCTTAGGCATGGTAAAGAAACCCACACCCTTGGGTACCCTCCCGATTCCAGAACCACCCGTGACTTTGATGGAAAATACCTTCTTTCATAGGGTTCGAGTTAGAGTTTTTTCTGATATTCACTATGAGAGATGTGGATGGTGGTGTTGAGGATGGAGACATGATAATGTAAATTTGAGGATCGGGAGTGGGGAGTAATCATCGTCCCCAGTCATATCAAATGTCACATTTGCTCTCCGTATGAGTGGGGGGCACAGGGGGATCGGGAGTGGGGAGTAATCATCGTCCCCAGTCATATCAAATGTCACATTTGCTCTCCGTATGAGTGGGGGGCACAGGGCAGAGCCTTTACTATGTTGGAACCAATTGGGAGATGTAGGATTCAGAGATTGATTCAACCTCTGTGAGAGGTTTGGGTGCGGCCCATATCTCTGAGTTGCTTATGTGGGTGGGCCTTTGTCCCTTTTTTCTTCCCCCGGTATTTGTTGTTCTTTCATTTCCGGTTTTATTGTTGGAGTTGTTAGGTGTTATTTTGAGTGTGTGGGTGGGGTGCCTTTTGTTGTCTGGGGTTTTCCTTTCTTTTTTTGGTTTTGGTGCTCGGTTGGCGTGTCCTGCGTTGGGTTCTTCCATCCCAAGATTGTTTGTGATTCTTTTTCCAGGTGTTAATATATCCTTTTGAAATTAAAAAAAAAATGTCGCATTTCTCTTTTTCCAGGTGTTAATATATCCTTTTGAAATTAAAAAAAAAATGTCGCATTTCATGGAGTTGATAATACTCATTTTAGTATTAATTAGGCTAGTTTCCATTGAACCAGCTTGCTAATTTTTTTTTTAAAGTCATTATAAGTTCCTTATTAGAGTTTTTTTTTTTTAGGTTCCTTTTCCTTATTAGAGTTGATACATGTTGTTTGTATATATTTCATGAATAAAGATTGTATTATGTAGGTTAACAATGCGGGGATTACCGGAGTTGAAATTAAAGACCGTGATTTATTAACTTCCATCATTACGAGTGGGGTTAGTATATACAAACTTTGAAACTTACCTATGTTGTGTATCTATTGAATAAAATTTTAAAGTTGATTGCATCTGCACCGAATGGATTCTGATTATTTTTTATTTTTTACCAACTTGAACATCCATCATGCATGTATACCGGAAATTGTATTAAGATTTTTTACTAACCAAAACACTAAATTTCAATATTTCCCATTAAGCATTATTTTACTCGATAACTAACAATAATACAATGAAGATTCTTATTGTTTCTGGTCATATCTGTGTAGCACTATGACATTCTTATAGACAATGTTAAATGTCAACCTAATTCTGTAAGAGAATCTTTAGTATCATGGAATATATCGGGTGTTTTAATTTATTTGGTCTTGCAGCACGATGATATGTTTATAGGTGGAGTATGAATTCTCTATAGTGGCGGCACCAATGCAAGTGGTTGCACTGATGCAATAATTTCAAAACCGTTGGATCTGATTGTAAGTGGATATCCAACGATTTTGTAGCCACCTTACCATCATTGCACAAGATAAATGTCATGTTTTTTTTTTAACATCAATTTCATGATTTTTTTTTTTAACATCAATGTCATGATATATTCATTGTAGCCCACAAATCTAAAGTAAAAGCATTGGTTTTCTCCCTTCCCTGTGGATAAACCACCTCACTACATGAAAATGTCATGATATATATGTGATGTACTAGTCTATAAGTTGTATTGTGAAATTTGAAATTGTGTTGCAAGCTTTTCTATGTTTACTACGTTGAATATAGTTTTGTTTTTAGACGGGGATATTTTTATTAGTCTTAATATCTAAAATGGTTTTGATTTTTGGGTTGTTCTCATCTTGCAGCCACTACCTGATGAAGAATTGAGAAGGGCAGTAACACAAACATTTGAGTTAGCTAAAGAATGCATTCAGATAATTTATTATGGTGCTAAAAGAACATTTGAATACCTTCTTCCCCTCCTTCAATTATCTGATTCACCAAGAGTAGTCAATGTATCATCATTTTACGGCAAGATAGAGGTAAGCTGATATGTACATCAATAAATTCTTAAGAATGAATAAGCTTGTAATTATAGTGTTTAAGAGCCATCACAAAAAAAAAAAAAGACTTATCAACAAAAGTTAATATTAATACTAGATTACTTAAATGTTCACCATATTTTGATAGAGCCTAACTTGAAAGTTAAGACTTATGAATAATGAATAAACTTAGAAATAGATTGAGTGAAACAAATTTTTTCAAGGGTTAGATATGATCTATTTCTAAAGTTTTGATTGAGCCTAACTTGAAATTTTTAAATTTTTCTAAGCACATTTTGTTATATAGTTTACGTTTGCACTCAATTTTCTTTAACCAAGTTTGCATTATCACTTATATTGATGTCAAACCAGCGTGTATCCAATGAATGGGCTAAAGGAGTCTTCAGTGATGTTGATAACCTCACAGAAGATAGAATAGATGAAGTGATAAAGGAGTTAATCAAAGATTTTGAAGAAGGGTCCTTAGAAAGAAAAGGTTGGCCTAGGTATGCCGCTGCCTACACTGTTGCTAAAGCTTCTGTGAATGCCTATACAAGAATTCTTGCAAAGAAATATCCTAATTTCTGCATCAATTGTGTTTGCCCTGGTTATGTTAAGACTGATATGACTAATAATAATGGTATATTGACAGTAGAAGAAGGAGCTGCAAATCCTGTTAGGTTGGCACTACTTCCCAATGATAGTCCATCTGGCCTTTTCTATAGTCAGAACCGAATCGATTCATTTTGATTAATATTATTTAGAGAATGTTCACAAGTGTCCTTATGTAGTGTTTATGTAATCCAAATTGGTAATTTTTGTAGAGAAAATGATGTATTTAATACTTAGACGGATGTTTGACACTTCATTATTATTTTCATTGATTTTGAAAATTCATTTTTACTAGACTCTCTTCTATCTACGACAATTGTTTAGTACATGAGTTTTTCGTATCTTTTTTTACGATTCAATATGTAAATTTTTAAAAACTGAAGATGTTTACTAAAAGTAAACGAGACCTAAGATGATTTTGCCTCAAGAACTTATTATACATAAGGCTATAATTTATGGTTTTTGGCTCTAGAATATTACATAAAAATTTATTGTTCAATTTACTTTTTGGATGAATCTATCTAAATATAAATCACTTTATATTTACCCCACTTTTAGTTAGAATCAATTTTATCAAATCAATTCACTCAATCAATTCTTGTTATCGCAAAACCAAGCATACACTAAGTGTGTGTACTTGTTGTGGAGTATATGAAAAATACTTTAATGATTTTAGTTTATTTTGATGAAGAATAATAGATTAAGGGTTAAAATAAATAATTTTAATTAAAGAAAATGATTGATTACAACAAAGATAGAAACAAAGTGAGTTGATTGGAACTCGGTGATATCTTGTCATATCTCTTTACTCTTTACTTTTAAAAAAGAAAAAGAAAAAGGAAAAGAAAAATAAGCTAAACTAGAATGGGATGAGTCTTTGAACATCTTCCCTTCATAAACAATGCCCATTCTCACATCTGTTCTCACAATTCACAAATCAAATCAATCCAATAACTCAAAATTATGTATATTTTTTTTTTTAGAAAAAAATTAGTATTAAAGATAATATAAGTAGTTATCCCTAAAGGGAGAAAATGAAGAAAAAAAATTAAATCAAAGGTTCTTATATTTTCTTTGGACATTCTAGGTGTGGCTATTGGAAGTGGATGGCAGGATTTGGTAGCTTATGTTAACTTGGCTGCTTATTATCTTGTTGGTCTCCCCATTGGTTGTGTCCTTGGTTTTAAAACAAGTTTGGGTGTAGCAGTAAGTTGAAAATACTTTAATATGTTTTTAAAAAAATTGAGACATTTTAATTTGCACCTTAACTTATGATTAAAAGAGGGTACATATACTTGGCTTTATATTTTTGTAGGGCATGTGGTGGGGCATGATTGTTGGCATTTTACTTCAAACATTGATTCTTATCGTTCTTACAATAAGAACAGATTGGGACAAAGAGGTAATGGGTTTAATTGTTATTATCAAATAAACTTGCTTTCTGCATAAGTTAATTGCTAAGTTTAAATATTTCTTTGTGAATAAAATTTTGGCAATAATATTTTTAATTTTTTTTTGTTCATCTTCCATTTTTGTTATTGATCGTGTAAAGTCTAAAGATGGAAAACTAGACTTAGTTGATAATATCTAAATTAAGAGGCAACTTGTAAATGGAGGCATTACCCTTTTTATTTTTTATGTAGGCAGTGAATTTTGAGAAGCTACTAAGTGGATAA
Protein sequence

>MS.gene009163.t1

MGEPTKRYAVVTGANKGIGFEIVRQLASAGIKVVLTARDETRGLHALETLKASGLSDFVVFHQLDVADASSVASLADFVKSQFGKLDILVNNAGITGVEIKDRDLLTSIITSGPLPDEELRRAVTQTFELAKECIQIIYYGAKRTFEYLLPLLQLSDSPRVVNVSSFYGKIERVSNEWAKGVFSDVDNLTEDRIDEVIKELIKDFEEGSLERKGWPRYAAAYTVAKASVNAYTRILAKKYPNFCINCVCPGYVKTDMTNNNGVAIGSGWQDLVAYVNLAAYYLVGLPIGCVLGFKTSLGVAGMWWGMIVGILLQTLILIVLTIRTDWDKEAVNFEKLLSG