AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008719

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008719.t1 MTR_4g087460 93.730 319 12 2 1 318 1 312 0.0 598
MS.gene008719.t1 MTR_3g098470 42.384 302 148 9 16 310 29 311 6.63e-75 233
MS.gene008719.t1 MTR_1g031180 40.333 300 157 8 16 310 34 316 3.35e-67 213
MS.gene008719.t1 MTR_8g096060 40.333 300 154 9 16 310 31 310 1.70e-66 211
MS.gene008719.t1 MTR_7g076660 40.614 293 139 8 24 303 9 279 7.95e-64 203
MS.gene008719.t1 MTR_7g076660 40.614 293 139 8 24 303 10 280 9.52e-64 203
MS.gene008719.t1 MTR_7g076655 39.590 293 142 8 24 303 9 279 1.27e-59 192
MS.gene008719.t1 MTR_8g031980 36.808 307 147 8 21 302 6 290 1.08e-58 191
MS.gene008719.t1 MTR_5g062280 38.436 307 143 7 19 302 4 287 1.32e-58 190
MS.gene008719.t1 MTR_7g076660 38.406 276 135 8 41 303 2 255 1.00e-54 179
MS.gene008719.t1 MTR_8g032000 36.842 304 145 8 24 302 9 290 2.41e-54 179
MS.gene008719.t1 MTR_8g032010 34.713 314 157 9 20 308 5 295 5.22e-54 179
MS.gene008719.t1 MTR_8g031960 35.738 305 149 8 23 302 9 291 7.86e-54 178
MS.gene008719.t1 MTR_8g031610 37.134 307 145 10 24 306 7 289 8.82e-53 175
MS.gene008719.t1 MTR_8g031910 37.919 298 149 9 19 302 4 279 1.27e-52 174
MS.gene008719.t1 MTR_8g031990 33.228 316 164 8 20 310 5 298 8.93e-52 173
MS.gene008719.t1 MTR_8g031940 36.066 305 147 9 24 302 9 291 1.50e-51 172
MS.gene008719.t1 MTR_8g031600 37.459 307 145 9 22 303 7 291 1.01e-50 170
MS.gene008719.t1 MTR_8g031630 37.338 308 144 11 24 306 9 292 1.89e-50 169
MS.gene008719.t1 MTR_8g032020 34.426 305 152 8 24 302 10 292 9.97e-50 167
MS.gene008719.t1 MTR_7g076665 35.880 301 149 8 24 302 9 287 1.02e-49 167
MS.gene008719.t1 MTR_8g031650 36.842 304 144 10 25 303 104 384 6.66e-49 168
MS.gene008719.t1 MTR_8g031650 32.500 120 66 4 169 285 1 108 3.57e-12 67.0
MS.gene008719.t1 MTR_3g079320 36.624 314 148 9 18 302 7 298 1.42e-48 165
MS.gene008719.t1 MTR_8g031900 34.528 307 159 9 24 310 9 293 1.53e-48 164
MS.gene008719.t1 MTR_8g031650 37.134 307 144 11 25 306 270 552 1.24e-47 168
MS.gene008719.t1 MTR_8g031650 37.282 287 134 9 24 285 9 274 8.46e-47 166
MS.gene008719.t1 MTR_7g106800 42.381 210 107 6 24 230 11 209 2.80e-41 145
MS.gene008719.t1 MTR_8g031850 31.597 288 155 8 24 310 9 255 9.90e-38 135
MS.gene008719.t1 MTR_5g083430 33.333 195 110 5 109 302 1 176 2.16e-28 108
MS.gene008719.t1 MTR_8g032030 38.674 181 77 5 21 176 6 177 4.01e-27 106
MS.gene008719.t1 MTR_6g041980 31.293 147 86 5 24 167 69 203 9.40e-14 70.5
MS.gene008719.t1 MTR_6g041980 31.507 146 85 5 25 167 70 203 2.10e-13 70.5
MS.gene008719.t1 MTR_4g105310 26.384 307 170 8 24 315 51 316 3.61e-12 66.6
MS.gene008719.t1 MTR_1g100315 29.032 217 122 5 24 240 32 216 9.72e-12 65.1
MS.gene008719.t1 MTR_4g105310 27.309 249 139 7 24 271 51 258 3.66e-11 63.2
MS.gene008719.t1 MTR_8g044240 35.345 116 68 3 25 137 75 186 7.01e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008719.t1 AT3G59710 58.983 295 101 6 10 303 6 281 9.11e-116 336
MS.gene008719.t1 AT3G59710 59.233 287 98 5 17 303 24 291 1.54e-114 333
MS.gene008719.t1 AT3G59710 59.375 288 98 5 16 303 42 310 3.03e-114 333
MS.gene008719.t1 AT5G61830 44.186 301 147 8 16 310 31 316 2.70e-72 226
MS.gene008719.t1 AT5G61830 44.186 301 147 8 16 310 31 316 2.70e-72 226
MS.gene008719.t1 AT5G51030 41.333 300 154 7 16 310 32 314 2.86e-71 223
MS.gene008719.t1 AT3G61220 36.455 299 151 6 24 302 9 288 9.77e-56 183
MS.gene008719.t1 AT3G61220 36.455 299 151 6 24 302 16 295 1.39e-55 183
MS.gene008719.t1 AT3G61220 36.455 299 151 6 24 302 40 319 2.46e-55 183
MS.gene008719.t1 AT1G01800 35.099 302 154 7 23 302 6 287 4.68e-53 176
MS.gene008719.t1 AT2G24190 34.921 315 158 8 8 302 6 293 4.70e-53 176
MS.gene008719.t1 AT2G24190 35.117 299 155 6 24 302 9 288 1.92e-52 174
MS.gene008719.t1 AT1G01800 31.771 192 112 4 112 302 79 252 3.44e-28 110
MS.gene008719.t1 AT4G09750 33.088 136 86 3 25 160 47 177 3.36e-13 69.7

Find 69 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAATAATTCATATAAATT+AGG 0.194089 4.4:+25273168 MS.gene008719:CDS
TCCTCCTTCAAAGACAAATT+TGG 0.232306 4.4:+25272025 MS.gene008719:CDS
TAGCTCAAGACTTGGGTCTT+TGG 0.300326 4.4:+25272813 MS.gene008719:CDS
AGACTATGCAGTATCAAAAT+TGG 0.327322 4.4:+25273104 MS.gene008719:CDS
GTCAAGGGTGGCCATCATAT+TGG 0.371559 4.4:+25273079 MS.gene008719:CDS
AGTGCAGGAGAAGGAAAATA+AGG 0.378888 4.4:-25271655 None:intergenic
GTTTAGAATTAGCAACTTGT+TGG 0.384253 4.4:-25273374 None:intergenic
GGCTCCAAATTTGTCTTTGA+AGG 0.388365 4.4:-25272029 None:intergenic
GAGAGCATTCGAGCGCAAAT+AGG 0.402815 4.4:+25271929 MS.gene008719:CDS
TCTAGAGAGTGAGGACTTGA+AGG 0.406286 4.4:+25272993 MS.gene008719:CDS
TTTCTAAGAGATGTGAGAAA+TGG 0.407157 4.4:+25273045 MS.gene008719:CDS
GTCATGAAAACAAATTTCTA+TGG 0.424036 4.4:+25272706 MS.gene008719:CDS
CTCAATGTTAGCTCAAGACT+TGG 0.436992 4.4:+25272805 MS.gene008719:CDS
GGAAGAAGAGATCAAGGAAA+TGG 0.444648 4.4:+25273014 MS.gene008719:CDS
ACTGCTAGAGACAGGCAAAA+AGG 0.447105 4.4:+25271893 MS.gene008719:CDS
GGAGAAGGAAAATAAGGTTC+TGG 0.450582 4.4:-25271649 None:intergenic
CAGGAGGTAACAAGGGCATT+GGG 0.450651 4.4:+25271822 MS.gene008719:CDS
AAGAAGAGGAGCAACGAAAG+AGG 0.458793 4.4:-25272759 None:intergenic
CTTGAAGGAAGAAGAGATCA+AGG 0.478401 4.4:+25273008 MS.gene008719:CDS
CTCCTGCACTTTGCTCAACC+AGG 0.481741 4.4:+25271668 MS.gene008719:CDS
TATGCAGAAGGTGACAAACT+TGG 0.485647 4.4:+25272957 MS.gene008719:intron
GTCTTTGAAGGAGGATGCAA+AGG 0.489278 4.4:-25272017 None:intergenic
CCGCCACACCACTTGCCTAC+CGG 0.490978 4.4:+25273318 MS.gene008719:CDS
AGAAGAGGAGCAACGAAAGA+GGG 0.498513 4.4:-25272758 None:intergenic
GAGACGGTAGCGGTGGTGAC+AGG 0.500973 4.4:+25271803 MS.gene008719:CDS
ACTCAAACCTCAATGACAAA+AGG 0.505232 4.4:+25273237 MS.gene008719:CDS
ATTGAGGTTTGAGTGAAGCC+AGG 0.506250 4.4:-25273228 None:intergenic
AACCTCAATGACAAAAGGTA+AGG 0.507566 4.4:+25273242 MS.gene008719:CDS
GATGGAAGAATTAGAAGAAG+AGG 0.514502 4.4:-25272773 None:intergenic
AGACAAATTTGGAGCCACCT+TGG 0.518797 4.4:+25272036 MS.gene008719:CDS
TGGTCCCAAGTTGCTTATAG+AGG 0.521817 4.4:+25272726 MS.gene008719:CDS
ACAGGAGGTAACAAGGGCAT+TGG 0.521906 4.4:+25271821 MS.gene008719:CDS
TCCAAATTTGTCTTTGAAGG+AGG 0.528089 4.4:-25272026 None:intergenic
GTGGTCGAAGGAGACGGTAG+CGG 0.531902 4.4:+25271793 MS.gene008719:CDS
TAGTGTTAACTGCTAGAGAC+AGG 0.539016 4.4:+25271885 MS.gene008719:CDS
TGAATGAAGGTGGTGGTCGA+AGG 0.543250 4.4:+25271781 MS.gene008719:intron
GCATAGTCTGTCCAATATGA+TGG 0.543911 4.4:-25273090 None:intergenic
TACCTGGTTGAGCAAAGTGC+AGG 0.547617 4.4:-25271670 None:intergenic
AACATTGAGAATACGTGTGA+TGG 0.548769 4.4:-25272791 None:intergenic
AAATGCATACTCAAAAGTGT+TGG 0.552148 4.4:+25273131 MS.gene008719:CDS
AATCAGCATCTGAGACATCA+AGG 0.556283 4.4:-25271985 None:intergenic
AGAATTAGCAACTTGTTGGA+AGG 0.559112 4.4:-25273370 None:intergenic
AAGTGCCTCTATAAGCAACT+TGG 0.563609 4.4:-25272731 None:intergenic
CCGGTAGGCAAGTGGTGTGG+CGG 0.566565 4.4:-25273318 None:intergenic
GAAAAGAATTCTAGAGAGTG+AGG 0.571043 4.4:+25272984 MS.gene008719:CDS
AGTGCCTCTATAAGCAACTT+GGG 0.573541 4.4:-25272730 None:intergenic
GAAGAGGAGCAACGAAAGAG+GGG 0.583471 4.4:-25272757 None:intergenic
TCAATGTTAGCTCAAGACTT+GGG 0.584941 4.4:+25272806 MS.gene008719:CDS
GGTGGTGACAGGAGGTAACA+AGG 0.585644 4.4:+25271814 MS.gene008719:CDS
ATGGAACATGGAAGAGTCAA+GGG 0.592718 4.4:+25273064 MS.gene008719:CDS
TTGAGGTTTGAGTGAAGCCA+GGG 0.599643 4.4:-25273227 None:intergenic
GTCGAAGGAGACGGTAGCGG+TGG 0.601525 4.4:+25271796 MS.gene008719:CDS
AATGGAACATGGAAGAGTCA+AGG 0.605080 4.4:+25273063 MS.gene008719:CDS
GTTGAGCAAAGTGCAGGAGA+AGG 0.605589 4.4:-25271664 None:intergenic
CATGGTCCACTGAATTCTCG+TGG 0.607377 4.4:-25272675 None:intergenic
GGTGTGGCGGAAGCAATACA+AGG 0.610060 4.4:-25273305 None:intergenic
GAGATGTGAGAAATGGAACA+TGG 0.612549 4.4:+25273052 MS.gene008719:CDS
AATCCATACGAGAATATCCA+AGG 0.627744 4.4:-25272053 None:intergenic
ACCTCAATGACAAAAGGTAA+GGG 0.629438 4.4:+25273243 MS.gene008719:CDS
TGGCGGAAGCAATACAAGGA+TGG 0.635034 4.4:-25273301 None:intergenic
TTTCCGGTAGGCAAGTGGTG+TGG 0.643344 4.4:-25273321 None:intergenic
ACTAGAAAGTGAACATGATG+AGG 0.645794 4.4:-25271962 None:intergenic
ACGGTAGCGGTGGTGACAGG+AGG 0.653942 4.4:+25271806 MS.gene008719:CDS
AAGGTGGTGGTCGAAGGAGA+CGG 0.656000 4.4:+25271787 MS.gene008719:intron
GAACATGATGAGGTGCAACA+AGG 0.658083 4.4:-25271952 None:intergenic
TGAGCTCCACGAGAATTCAG+TGG 0.667471 4.4:+25272669 MS.gene008719:CDS
GTGGTGACAGGAGGTAACAA+GGG 0.695952 4.4:+25271815 MS.gene008719:CDS
GAACATGGAAGAGTCAAGGG+TGG 0.718135 4.4:+25273067 MS.gene008719:CDS
CCATACGAGAATATCCAAGG+TGG 0.741974 4.4:-25272050 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATAAAAATTATA+TGG + chr4.4:25272900-25272919 MS.gene008719:intron 0.0%
!! ATATTAATCAAACATATATA+TGG - chr4.4:25271731-25271750 None:intergenic 10.0%
!! AAGTAAATTCATGAATTATT+AGG - chr4.4:25272848-25272867 None:intergenic 15.0%
!! AATAAAATTGAATAAGTAGA+AGG - chr4.4:25272084-25272103 None:intergenic 15.0%
!! TAAATATTATTATATGCAGA+AGG + chr4.4:25272945-25272964 MS.gene008719:intron 15.0%
!!! AATTAATTTTTTATGTGATC+AGG + chr4.4:25272621-25272640 MS.gene008719:intron 15.0%
!! GTGAATAAAAAATTACAACT+AGG - chr4.4:25272577-25272596 None:intergenic 20.0%
!!! AAAAGCAAAAAATATTGACT+TGG + chr4.4:25272297-25272316 MS.gene008719:intron 20.0%
!!! AAAGCAAAAAATATTGACTT+GGG + chr4.4:25272298-25272317 MS.gene008719:intron 20.0%
!!! CACATAAATATGTTTTTGAA+AGG + chr4.4:25272594-25272613 MS.gene008719:intron 20.0%
! AATGACACATTTATTACTAG+AGG - chr4.4:25272145-25272164 None:intergenic 25.0%
! AGTAACTAATATGGATAGAT+CGG + chr4.4:25272330-25272349 MS.gene008719:intron 25.0%
! ATTCATGAATTTACTTTACC+AGG + chr4.4:25272852-25272871 MS.gene008719:intron 25.0%
! GTCATGAAAACAAATTTCTA+TGG + chr4.4:25272706-25272725 MS.gene008719:CDS 25.0%
! GTTTCACAATGATAAACATT+TGG + chr4.4:25272187-25272206 MS.gene008719:intron 25.0%
! TATTTGTGCTAAATGAATGA+AGG + chr4.4:25271768-25271787 MS.gene008719:intron 25.0%
! TTATCATTGTGAAACTAAGA+GGG - chr4.4:25272182-25272201 None:intergenic 25.0%
! TTTACCAGGTTAATATAAGT+TGG + chr4.4:25272866-25272885 MS.gene008719:intron 25.0%
! TTTATCATTGTGAAACTAAG+AGG - chr4.4:25272183-25272202 None:intergenic 25.0%
AGACTATGCAGTATCAAAAT+TGG + chr4.4:25273104-25273123 MS.gene008719:CDS 30.0%
ATAGTAGGATTGAAAAGATG+AGG - chr4.4:25271696-25271715 None:intergenic 30.0%
CATAGTTCGAGTAACTAATA+TGG + chr4.4:25272321-25272340 MS.gene008719:intron 30.0%
CTTTGGTCAAAAAATTGTCT+AGG - chr4.4:25272536-25272555 None:intergenic 30.0%
TGGCATTAAATGTGTATAGT+AGG - chr4.4:25271711-25271730 None:intergenic 30.0%
! AAATGCATACTCAAAAGTGT+TGG + chr4.4:25273131-25273150 MS.gene008719:CDS 30.0%
! GTTTAGAATTAGCAACTTGT+TGG - chr4.4:25273377-25273396 None:intergenic 30.0%
! TTTCTAAGAGATGTGAGAAA+TGG + chr4.4:25273045-25273064 MS.gene008719:CDS 30.0%
!! AACCAAGATAGTGTTGATAA+TGG + chr4.4:25272438-25272457 MS.gene008719:intron 30.0%
!! ACCAAGATAGTGTTGATAAT+GGG + chr4.4:25272439-25272458 MS.gene008719:intron 30.0%
AACATTGAGAATACGTGTGA+TGG - chr4.4:25272794-25272813 None:intergenic 35.0%
AACCTCAATGACAAAAGGTA+AGG + chr4.4:25273242-25273261 MS.gene008719:CDS 35.0%
AATCCATACGAGAATATCCA+AGG - chr4.4:25272056-25272075 None:intergenic 35.0%
ACCCATTATCAACACTATCT+TGG - chr4.4:25272443-25272462 None:intergenic 35.0%
ACCTCAATGACAAAAGGTAA+GGG + chr4.4:25273243-25273262 MS.gene008719:CDS 35.0%
ACTAGAAAGTGAACATGATG+AGG - chr4.4:25271965-25271984 None:intergenic 35.0%
ACTCAAACCTCAATGACAAA+AGG + chr4.4:25273237-25273256 MS.gene008719:CDS 35.0%
GACACATTTATTACTAGAGG+TGG - chr4.4:25272142-25272161 None:intergenic 35.0%
GATGGAAGAATTAGAAGAAG+AGG - chr4.4:25272776-25272795 None:intergenic 35.0%
GTGACCAACTTATATTAACC+TGG - chr4.4:25272873-25272892 None:intergenic 35.0%
TCAATGTTAGCTCAAGACTT+GGG + chr4.4:25272806-25272825 MS.gene008719:CDS 35.0%
TCCTCCTTCAAAGACAAATT+TGG + chr4.4:25272025-25272044 MS.gene008719:CDS 35.0%
TTGTGCTAAATGAATGAAGG+TGG + chr4.4:25271771-25271790 MS.gene008719:intron 35.0%
! CAACACTATCTTGGTTTGAA+AGG - chr4.4:25272434-25272453 None:intergenic 35.0%
! GAAAAGAATTCTAGAGAGTG+AGG + chr4.4:25272984-25273003 MS.gene008719:CDS 35.0%
! TCCAAATTTGTCTTTGAAGG+AGG - chr4.4:25272029-25272048 None:intergenic 35.0%
! TCTGAGCAAGAAGAATTTTC+CGG - chr4.4:25273340-25273359 None:intergenic 35.0%
! TTTTCATGACGTTTTCAGCA+TGG - chr4.4:25272696-25272715 None:intergenic 35.0%
!! AGAATTAGCAACTTGTTGGA+AGG - chr4.4:25273373-25273392 None:intergenic 35.0%
AAGTGCCTCTATAAGCAACT+TGG - chr4.4:25272734-25272753 None:intergenic 40.0%
AATGGAACATGGAAGAGTCA+AGG + chr4.4:25273063-25273082 MS.gene008719:CDS 40.0%
AGTGCAGGAGAAGGAAAATA+AGG - chr4.4:25271658-25271677 None:intergenic 40.0%
AGTGCCTCTATAAGCAACTT+GGG - chr4.4:25272733-25272752 None:intergenic 40.0%
ATGGAACATGGAAGAGTCAA+GGG + chr4.4:25273064-25273083 MS.gene008719:CDS 40.0%
CTCAATGTTAGCTCAAGACT+TGG + chr4.4:25272805-25272824 MS.gene008719:CDS 40.0%
CTTGAAGGAAGAAGAGATCA+AGG + chr4.4:25273008-25273027 MS.gene008719:CDS 40.0%
GAGATGTGAGAAATGGAACA+TGG + chr4.4:25273052-25273071 MS.gene008719:CDS 40.0%
GCATAGTCTGTCCAATATGA+TGG - chr4.4:25273093-25273112 None:intergenic 40.0%
GGAAGAAGAGATCAAGGAAA+TGG + chr4.4:25273014-25273033 MS.gene008719:CDS 40.0%
GGAGAAGGAAAATAAGGTTC+TGG - chr4.4:25271652-25271671 None:intergenic 40.0%
GGCTCCAAATTTGTCTTTGA+AGG - chr4.4:25272032-25272051 None:intergenic 40.0%
TAGTGTTAACTGCTAGAGAC+AGG + chr4.4:25271885-25271904 MS.gene008719:CDS 40.0%
TATGCAGAAGGTGACAAACT+TGG + chr4.4:25272957-25272976 MS.gene008719:intron 40.0%
TGCTAAATGAATGAAGGTGG+TGG + chr4.4:25271774-25271793 MS.gene008719:intron 40.0%
! AATCAGCATCTGAGACATCA+AGG - chr4.4:25271988-25272007 None:intergenic 40.0%
! AGCAAGAAGAATTTTCCGGT+AGG - chr4.4:25273336-25273355 None:intergenic 40.0%
! GATTGAAAAGATGAGGTACC+TGG - chr4.4:25271689-25271708 None:intergenic 40.0%
! GTGAAACGTTTTGCTGAGTT+GGG + chr4.4:25271854-25271873 MS.gene008719:CDS 40.0%
! TGTGAAACGTTTTGCTGAGT+TGG + chr4.4:25271853-25271872 MS.gene008719:CDS 40.0%
AAGAAGAGGAGCAACGAAAG+AGG - chr4.4:25272762-25272781 None:intergenic 45.0%
ACTAGGTCCATGTCTAGTCT+TGG - chr4.4:25272560-25272579 None:intergenic 45.0%
ACTGCTAGAGACAGGCAAAA+AGG + chr4.4:25271893-25271912 MS.gene008719:CDS 45.0%
AGAAGAGGAGCAACGAAAGA+GGG - chr4.4:25272761-25272780 None:intergenic 45.0%
ATTGAGGTTTGAGTGAAGCC+AGG - chr4.4:25273231-25273250 None:intergenic 45.0%
CCAAAGACCAAGACTAGACA+TGG + chr4.4:25272550-25272569 MS.gene008719:intron 45.0%
CCACCTTGGATATTCTCGTA+TGG + chr4.4:25272050-25272069 MS.gene008719:intron 45.0%
CCATACGAGAATATCCAAGG+TGG - chr4.4:25272053-25272072 None:intergenic 45.0%
GAACATGATGAGGTGCAACA+AGG - chr4.4:25271955-25271974 None:intergenic 45.0%
TAGAGACAGGCAAAAAGGAG+AGG + chr4.4:25271898-25271917 MS.gene008719:CDS 45.0%
TAGCTCAAGACTTGGGTCTT+TGG + chr4.4:25272813-25272832 MS.gene008719:CDS 45.0%
TCTAGAGAGTGAGGACTTGA+AGG + chr4.4:25272993-25273012 MS.gene008719:CDS 45.0%
TGGTCCCAAGTTGCTTATAG+AGG + chr4.4:25272726-25272745 MS.gene008719:CDS 45.0%
TTGAGGTTTGAGTGAAGCCA+GGG - chr4.4:25273230-25273249 None:intergenic 45.0%
! AGAATTTTCCGGTAGGCAAG+TGG - chr4.4:25273329-25273348 None:intergenic 45.0%
! AGACAAATTTGGAGCCACCT+TGG + chr4.4:25272036-25272055 MS.gene008719:CDS 45.0%
! GCCCTTACCTTTTGTCATTG+AGG - chr4.4:25273247-25273266 None:intergenic 45.0%
! GTCTTTGAAGGAGGATGCAA+AGG - chr4.4:25272020-25272039 None:intergenic 45.0%
!! CCATGTCTAGTCTTGGTCTT+TGG - chr4.4:25272553-25272572 None:intergenic 45.0%
!!! AGTGTGAACTGTTTTTGCCC+TGG + chr4.4:25273210-25273229 MS.gene008719:CDS 45.0%
!! ATTAATAATTCATATAAATT+AGG + chr4.4:25273168-25273187 MS.gene008719:CDS 5.0%
CATGGTCCACTGAATTCTCG+TGG - chr4.4:25272678-25272697 None:intergenic 50.0%
GAACATGGAAGAGTCAAGGG+TGG + chr4.4:25273067-25273086 MS.gene008719:CDS 50.0%
GAAGAGGAGCAACGAAAGAG+GGG - chr4.4:25272760-25272779 None:intergenic 50.0%
GCAAAAAGGAGAGGATGCTG+TGG + chr4.4:25271907-25271926 MS.gene008719:CDS 50.0%
GTCAAGGGTGGCCATCATAT+TGG + chr4.4:25273079-25273098 MS.gene008719:CDS 50.0%
GTGGTGACAGGAGGTAACAA+GGG + chr4.4:25271815-25271834 MS.gene008719:CDS 50.0%
GTTGAGCAAAGTGCAGGAGA+AGG - chr4.4:25271667-25271686 None:intergenic 50.0%
TACCTGGTTGAGCAAAGTGC+AGG - chr4.4:25271673-25271692 None:intergenic 50.0%
TGAGCTCCACGAGAATTCAG+TGG + chr4.4:25272669-25272688 MS.gene008719:CDS 50.0%
TGGCGGAAGCAATACAAGGA+TGG - chr4.4:25273304-25273323 None:intergenic 50.0%
! ACAGGAGGTAACAAGGGCAT+TGG + chr4.4:25271821-25271840 MS.gene008719:CDS 50.0%
! TGAATGAAGGTGGTGGTCGA+AGG + chr4.4:25271781-25271800 MS.gene008719:intron 50.0%
!! CAGGAGGTAACAAGGGCATT+GGG + chr4.4:25271822-25271841 MS.gene008719:CDS 50.0%
!! GAGAGCATTCGAGCGCAAAT+AGG + chr4.4:25271929-25271948 MS.gene008719:CDS 50.0%
CTCCTGCACTTTGCTCAACC+AGG + chr4.4:25271668-25271687 MS.gene008719:CDS 55.0%
GGTGGTGACAGGAGGTAACA+AGG + chr4.4:25271814-25271833 MS.gene008719:CDS 55.0%
TTTCCGGTAGGCAAGTGGTG+TGG - chr4.4:25273324-25273343 None:intergenic 55.0%
! AAGGTGGTGGTCGAAGGAGA+CGG + chr4.4:25271787-25271806 MS.gene008719:intron 55.0%
! GGTGTGGCGGAAGCAATACA+AGG - chr4.4:25273308-25273327 None:intergenic 55.0%
GTGGTCGAAGGAGACGGTAG+CGG + chr4.4:25271793-25271812 MS.gene008719:CDS 60.0%
CCGCCACACCACTTGCCTAC+CGG + chr4.4:25273318-25273337 MS.gene008719:CDS 65.0%
CCGGTAGGCAAGTGGTGTGG+CGG - chr4.4:25273321-25273340 None:intergenic 65.0%
! ACGGTAGCGGTGGTGACAGG+AGG + chr4.4:25271806-25271825 MS.gene008719:CDS 65.0%
! GAGACGGTAGCGGTGGTGAC+AGG + chr4.4:25271803-25271822 MS.gene008719:CDS 65.0%
! GTCGAAGGAGACGGTAGCGG+TGG + chr4.4:25271796-25271815 MS.gene008719:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 25271643 25273401 25271643 ID=MS.gene008719
chr4.4 mRNA 25271643 25273401 25271643 ID=MS.gene008719.t1;Parent=MS.gene008719
chr4.4 exon 25271643 25271689 25271643 ID=MS.gene008719.t1.exon1;Parent=MS.gene008719.t1
chr4.4 CDS 25271643 25271689 25271643 ID=cds.MS.gene008719.t1;Parent=MS.gene008719.t1
chr4.4 exon 25271790 25272066 25271790 ID=MS.gene008719.t1.exon2;Parent=MS.gene008719.t1
chr4.4 CDS 25271790 25272066 25271790 ID=cds.MS.gene008719.t1;Parent=MS.gene008719.t1
chr4.4 exon 25272643 25272837 25272643 ID=MS.gene008719.t1.exon3;Parent=MS.gene008719.t1
chr4.4 CDS 25272643 25272837 25272643 ID=cds.MS.gene008719.t1;Parent=MS.gene008719.t1
chr4.4 exon 25272964 25273401 25272964 ID=MS.gene008719.t1.exon4;Parent=MS.gene008719.t1
chr4.4 CDS 25272964 25273401 25272964 ID=cds.MS.gene008719.t1;Parent=MS.gene008719.t1
Gene Sequence

>MS.gene008719

ATGGAGCCAGAACCTTATTTTCCTTCTCCTGCACTTTGCTCAACCAGGTACCTCATCTTTTCAATCCTACTATACACATTTAATGCCATATATATGTTTGATTAATATTTGCTTTTGCTTGTGTTTATTTGTGCTAAATGAATGAAGGTGGTGGTCGAAGGAGACGGTAGCGGTGGTGACAGGAGGTAACAAGGGCATTGGGTTTGCTCTTGTGAAACGTTTTGCTGAGTTGGGACTGAGTGTAGTGTTAACTGCTAGAGACAGGCAAAAAGGAGAGGATGCTGTGGAGAGCATTCGAGCGCAAATAGGCCTTGTTGCACCTCATCATGTTCACTTTCTAGTCCTTGATGTCTCAGATGCTGATTCTATTAAAACCTTTGCATCCTCCTTCAAAGACAAATTTGGAGCCACCTTGGATATTCTCGTATGGATTAATTACCTTCTACTTATTCAATTTTATTTTTTACATCATTTTGATTGATTTTATTCTGTCTCTCCACCTCTAGTAATAAATGTGTCATTTTATATATTAATTACCCTCTTAGTTTCACAATGATAAACATTTGGTTATTTCACAAATATATAAATAAAAGAGAAAAAATAATATTTTTAGTAAATTTATCAAGTATTTTGTATTCACAAGTATCATAAATGAAAAGCAAAAAATATTGACTTGGGCATAGTTCGAGTAACTAATATGGATAGATCGGATACAAGTCAAAAAATAAAATTAAGTTTACTCACATGACTCGAACTTGAGACTTTATTTAAAAGACATGAGTTCTTTTCCTTTCAAACCAAGATAGTGTTGATAATGGGTTTCTAACAACATTATCTGTTGTGTTAAACAAAATTATAAGATACAATAAGATATAAATACACACGTTAGACCTAGACAATTTTTTGACCAAAGACCAAGACTAGACATGGACCTAGTTGTAATTTTTTATTCACATAAATATGTTTTTGAAAGGATCTAATTAATTTTTTATGTGATCAGGTGAACAATGCAGCTGTATCATTCAATGAGCTCCACGAGAATTCAGTGGACCATGCTGAAAACGTCATGAAAACAAATTTCTATGGTCCCAAGTTGCTTATAGAGGCACTTCTTCCCCTCTTTCGTTGCTCCTCTTCTTCTAATTCTTCCATCACACGTATTCTCAATGTTAGCTCAAGACTTGGGTCTTTGGATGTAAGTACCTAATAATTCATGAATTTACTTTACCAGGTTAATATAAGTTGGTCACATGCATAAAAAAAAAATAAAAATTATATGGCATATTCTATGCGTGCTCAAACTAAATATTATTATATGCAGAAGGTGACAAACTTGGAAATGAAAAGAATTCTAGAGAGTGAGGACTTGAAGGAAGAAGAGATCAAGGAAATGGTGAGAAAATTTCTAAGAGATGTGAGAAATGGAACATGGAAGAGTCAAGGGTGGCCATCATATTGGACAGACTATGCAGTATCAAAATTGGCTCTAAATGCATACTCAAAAGTGTTGGCTAAGAGATATAACATTAATAATTCATATAAATTAGGAAATACAACAAAGTTGATGAGTGTGAACTGTTTTTGCCCTGGCTTCACTCAAACCTCAATGACAAAAGGTAAGGGCACACACACTGCTGATCAAGCTGCTTCTCTTGCTACCATCCTTGTATTGCTTCCGCCACACCACTTGCCTACCGGAAAATTCTTCTTGCTCAGAAATAATATCACCTTCCAACAAGTTGCTAATTCTAAACTTTGA
Protein sequence

>MS.gene008719.t1

MEPEPYFPSPALCSTRWWSKETVAVVTGGNKGIGFALVKRFAELGLSVVLTARDRQKGEDAVESIRAQIGLVAPHHVHFLVLDVSDADSIKTFASSFKDKFGATLDILVNNAAVSFNELHENSVDHAENVMKTNFYGPKLLIEALLPLFRCSSSSNSSITRILNVSSRLGSLDKVTNLEMKRILESEDLKEEEIKEMVRKFLRDVRNGTWKSQGWPSYWTDYAVSKLALNAYSKVLAKRYNINNSYKLGNTTKLMSVNCFCPGFTQTSMTKGKGTHTADQAASLATILVLLPPHHLPTGKFFLLRNNITFQQVANSKL