AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26577

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26577.t1 MTR_4g087460 94.044 319 11 2 1 318 1 312 0.0 613
MS.gene26577.t1 MTR_3g098470 41.391 302 151 9 16 310 29 311 4.19e-73 228
MS.gene26577.t1 MTR_1g031180 39.667 300 159 8 16 310 34 316 3.59e-66 210
MS.gene26577.t1 MTR_8g096060 39.667 300 156 9 16 310 31 310 2.53e-65 208
MS.gene26577.t1 MTR_7g076660 39.333 300 147 8 24 310 9 286 5.54e-63 201
MS.gene26577.t1 MTR_7g076660 39.333 300 147 8 24 310 10 287 7.64e-63 201
MS.gene26577.t1 MTR_7g076655 38.333 300 150 8 24 310 9 286 4.89e-59 191
MS.gene26577.t1 MTR_5g062280 37.785 307 145 7 19 302 4 287 1.23e-57 188
MS.gene26577.t1 MTR_8g031980 36.156 307 149 8 21 302 6 290 1.37e-57 188
MS.gene26577.t1 MTR_8g032000 36.513 304 146 8 24 302 9 290 4.17e-54 179
MS.gene26577.t1 MTR_7g076660 37.102 283 143 8 41 310 2 262 6.30e-54 177
MS.gene26577.t1 MTR_8g032010 34.304 309 156 8 20 303 5 291 1.95e-53 177
MS.gene26577.t1 MTR_8g031960 35.082 305 151 8 23 302 9 291 7.78e-53 176
MS.gene26577.t1 MTR_8g031610 36.013 311 152 9 24 310 7 294 2.47e-52 174
MS.gene26577.t1 MTR_8g031990 32.911 316 165 8 20 310 5 298 3.00e-52 174
MS.gene26577.t1 MTR_8g031910 36.893 309 153 10 19 310 4 287 5.51e-52 173
MS.gene26577.t1 MTR_8g031940 34.824 313 156 9 24 310 9 299 1.39e-50 170
MS.gene26577.t1 MTR_8g031630 36.538 312 150 10 24 310 9 297 1.40e-50 169
MS.gene26577.t1 MTR_8g031600 37.134 307 146 9 22 303 7 291 2.41e-50 169
MS.gene26577.t1 MTR_8g032020 33.546 313 160 8 24 310 10 300 4.77e-50 168
MS.gene26577.t1 MTR_8g031650 36.334 311 150 10 25 310 104 391 4.60e-49 168
MS.gene26577.t1 MTR_8g031650 32.500 120 66 4 169 285 1 108 3.48e-12 67.0
MS.gene26577.t1 MTR_7g076665 35.216 301 151 8 24 302 9 287 8.86e-49 165
MS.gene26577.t1 MTR_3g079320 36.306 314 149 9 18 302 7 298 3.02e-48 164
MS.gene26577.t1 MTR_8g031900 34.667 300 154 9 24 303 9 286 9.69e-48 162
MS.gene26577.t1 MTR_8g031650 36.334 311 150 10 25 310 270 557 1.03e-47 168
MS.gene26577.t1 MTR_8g031650 37.282 287 134 9 24 285 9 274 9.48e-47 166
MS.gene26577.t1 MTR_7g106800 41.429 210 109 6 24 230 11 209 2.29e-40 142
MS.gene26577.t1 MTR_8g031850 31.673 281 150 8 24 303 9 248 5.43e-37 133
MS.gene26577.t1 MTR_5g083430 32.821 195 111 5 109 302 1 176 8.79e-28 107
MS.gene26577.t1 MTR_8g032030 38.122 181 78 5 21 176 6 177 8.37e-27 105
MS.gene26577.t1 MTR_6g041980 31.293 147 86 5 24 167 69 203 1.09e-13 70.5
MS.gene26577.t1 MTR_6g041980 31.507 146 85 5 25 167 70 203 2.83e-13 70.1
MS.gene26577.t1 MTR_4g105310 25.733 307 172 8 24 315 51 316 9.72e-12 65.5
MS.gene26577.t1 MTR_1g100315 29.032 217 122 5 24 240 32 216 4.35e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26577.t1 AT3G59710 57.966 295 104 6 10 303 6 281 2.31e-114 332
MS.gene26577.t1 AT3G59710 58.188 287 101 5 17 303 24 291 3.17e-113 330
MS.gene26577.t1 AT3G59710 58.333 288 101 5 16 303 42 310 5.88e-113 330
MS.gene26577.t1 AT5G61830 43.189 301 150 8 16 310 31 316 1.77e-70 221
MS.gene26577.t1 AT5G61830 43.189 301 150 8 16 310 31 316 1.77e-70 221
MS.gene26577.t1 AT5G51030 39.667 300 159 7 16 310 32 314 3.32e-69 218
MS.gene26577.t1 AT3G61220 36.482 307 156 6 24 310 9 296 5.55e-57 186
MS.gene26577.t1 AT3G61220 36.482 307 156 6 24 310 16 303 7.44e-57 186
MS.gene26577.t1 AT3G61220 36.482 307 156 6 24 310 40 327 1.41e-56 186
MS.gene26577.t1 AT2G24190 34.675 323 164 8 8 310 6 301 2.90e-53 177
MS.gene26577.t1 AT1G01800 34.516 310 161 7 23 310 6 295 6.81e-53 176
MS.gene26577.t1 AT2G24190 34.853 307 161 6 24 310 9 296 1.13e-52 175
MS.gene26577.t1 AT1G01800 31.000 200 119 4 112 310 79 260 6.75e-28 109
MS.gene26577.t1 AT4G09750 33.088 136 86 3 25 160 47 177 5.33e-13 68.9

Find 68 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAATAATTCATATAAATT+AGG 0.194089 4.1:+22717343 MS.gene26577:CDS
TCCTCCTTCAAAGACAAATT+TGG 0.232306 4.1:+22716216 MS.gene26577:CDS
AAGAAGAGGAGCAACGAAAT+AGG 0.257780 4.1:-22716934 None:intergenic
TAGCTCAAGACTTGGGTCTT+TGG 0.300326 4.1:+22716988 MS.gene26577:CDS
AGAAGAGGAGCAACGAAATA+GGG 0.320771 4.1:-22716933 None:intergenic
GTCAAGGGTGGCCATCATAT+TGG 0.371559 4.1:+22717254 MS.gene26577:CDS
AGTGCAGGAGAAGGAAAATA+AGG 0.378888 4.1:-22715838 None:intergenic
GTTTAGAATTAGCAACTTGT+TGG 0.384253 4.1:-22717549 None:intergenic
GAGACGGTGGCGGTGGTGAC+AGG 0.386563 4.1:+22715994 MS.gene26577:CDS
GGCTCCAAATTTGTCTTTGA+AGG 0.388365 4.1:-22716220 None:intergenic
TCTAGAGAGTGAGGACTTGA+AGG 0.406286 4.1:+22717168 MS.gene26577:CDS
TTTCTAAGAGATGTGAGAAA+TGG 0.407157 4.1:+22717220 MS.gene26577:CDS
GAGAGCATTCGAGCACAATT+AGG 0.410670 4.1:+22716120 MS.gene26577:CDS
GTCATGAAAACAAATTTCTA+TGG 0.424036 4.1:+22716881 MS.gene26577:CDS
CTCAATGTTAGCTCAAGACT+TGG 0.436992 4.1:+22716980 MS.gene26577:CDS
GGAAGAAGAGATCAAGGAAA+TGG 0.444648 4.1:+22717189 MS.gene26577:CDS
GGAGAAGGAAAATAAGGTTC+TGG 0.450582 4.1:-22715832 None:intergenic
CAGGAGGTAACAAGGGCATT+GGG 0.450651 4.1:+22716013 MS.gene26577:CDS
ACTGCTAGAGACAAGCAAAA+AGG 0.463408 4.1:+22716084 MS.gene26577:CDS
GTCGAAGGAGACGGTGGCGG+TGG 0.464837 4.1:+22715987 MS.gene26577:CDS
AGTGCCTCTATAAGCAACTT+AGG 0.466314 4.1:-22716905 None:intergenic
CCGCCACACCACTTGCCCAC+TGG 0.467591 4.1:+22717493 MS.gene26577:CDS
CTTGAAGGAAGAAGAGATCA+AGG 0.478401 4.1:+22717183 MS.gene26577:CDS
CTCCTGCACTTTGCTCAACC+AGG 0.481741 4.1:+22715851 MS.gene26577:CDS
TATGCAGAAGGTGACAAACT+TGG 0.485647 4.1:+22717132 MS.gene26577:intron
GTCTTTGAAGGAGGATGCAA+AGG 0.489278 4.1:-22716208 None:intergenic
TGGTCCTAAGTTGCTTATAG+AGG 0.503826 4.1:+22716901 MS.gene26577:CDS
ACTCAAACCTCAATGACAAA+AGG 0.505232 4.1:+22717412 MS.gene26577:CDS
ATTGAGGTTTGAGTGAAGCC+AGG 0.506250 4.1:-22717403 None:intergenic
AACCTCAATGACAAAAGGTA+AGG 0.507566 4.1:+22717417 MS.gene26577:CDS
GATGGAAGAATTAGAAGAAG+AGG 0.514502 4.1:-22716948 None:intergenic
GTGGTCGAAGGAGACGGTGG+CGG 0.516411 4.1:+22715984 MS.gene26577:CDS
AGACAAATTTGGAGCCACCT+TGG 0.518797 4.1:+22716227 MS.gene26577:CDS
GGTGGTGACAGGAGGTAACA+AGG 0.519926 4.1:+22716005 MS.gene26577:CDS
ACAGGAGGTAACAAGGGCAT+TGG 0.521906 4.1:+22716012 MS.gene26577:CDS
TCCAAATTTGTCTTTGAAGG+AGG 0.528089 4.1:-22716217 None:intergenic
TGAATGAAGGTGGTGGTCGA+AGG 0.543250 4.1:+22715972 MS.gene26577:intron
GCATAGTCTGTCCAATATGA+TGG 0.543911 4.1:-22717265 None:intergenic
AACATTGAGAATACGTGTGA+TGG 0.548769 4.1:-22716966 None:intergenic
AAATGCATACTCAAAAGTGT+TGG 0.552148 4.1:+22717306 MS.gene26577:CDS
AATCAGCATCTGAGACATCA+AGG 0.556283 4.1:-22716176 None:intergenic
GAAGAGGAGCAACGAAATAG+GGG 0.557798 4.1:-22716932 None:intergenic
AGAATTAGCAACTTGTTGGA+AGG 0.559112 4.1:-22717545 None:intergenic
TTTCCAGTGGGCAAGTGGTG+TGG 0.562735 4.1:-22717496 None:intergenic
TACCTGGTTGAGCAAAGTGC+AGG 0.563459 4.1:-22715853 None:intergenic
GAAAAGAATTCTAGAGAGTG+AGG 0.571043 4.1:+22717159 MS.gene26577:CDS
CCAGTGGGCAAGTGGTGTGG+CGG 0.576832 4.1:-22717493 None:intergenic
ATTAGCAACTTGTTGGAAGG+TGG 0.582292 4.1:-22717542 None:intergenic
TCAATGTTAGCTCAAGACTT+GGG 0.584941 4.1:+22716981 MS.gene26577:CDS
ACGGTGGCGGTGGTGACAGG+AGG 0.588347 4.1:+22715997 MS.gene26577:CDS
ATGGAACATGGAAGAGTCAA+GGG 0.592718 4.1:+22717239 MS.gene26577:CDS
TTGAGGTTTGAGTGAAGCCA+GGG 0.599643 4.1:-22717402 None:intergenic
AATGGAACATGGAAGAGTCA+AGG 0.605080 4.1:+22717238 MS.gene26577:CDS
GTTGAGCAAAGTGCAGGAGA+AGG 0.605589 4.1:-22715847 None:intergenic
ACTAGAAAGTGAAGATGATG+AGG 0.607346 4.1:-22716153 None:intergenic
CATGGTCCACTGAATTCTCG+TGG 0.607377 4.1:-22716850 None:intergenic
GAGATGTGAGAAATGGAACA+TGG 0.612549 4.1:+22717227 MS.gene26577:CDS
AATACATACGAGAATATCCA+AGG 0.618479 4.1:-22716244 None:intergenic
ACCTCAATGACAAAAGGTAA+GGG 0.629438 4.1:+22717418 MS.gene26577:CDS
TGGCGGAAGCAACACAAGGA+TGG 0.632160 4.1:-22717476 None:intergenic
GTGGTGGTCGAAGGAGACGG+TGG 0.633275 4.1:+22715981 MS.gene26577:CDS
AAGGTGGTGGTCGAAGGAGA+CGG 0.651263 4.1:+22715978 MS.gene26577:intron
GTGGTGACAGGAGGTAACAA+GGG 0.653515 4.1:+22716006 MS.gene26577:CDS
GAAGATGATGAGGTGCAACA+AGG 0.666269 4.1:-22716143 None:intergenic
TGAGCTCCACGAGAATTCAG+TGG 0.667471 4.1:+22716844 MS.gene26577:CDS
GGTGTGGCGGAAGCAACACA+AGG 0.673747 4.1:-22717480 None:intergenic
GAACATGGAAGAGTCAAGGG+TGG 0.718135 4.1:+22717242 MS.gene26577:CDS
ACATACGAGAATATCCAAGG+TGG 0.779700 4.1:-22716241 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAATTATA+TGG + chr4.1:22717075-22717094 MS.gene26577:intron 0.0%
!! TTTATGTGAATAAAAAATTA+TGG - chr4.1:22716757-22716776 None:intergenic 10.0%
!! AAGTAAATTCATGAATTATT+AGG - chr4.1:22717023-22717042 None:intergenic 15.0%
!! AATAAAATTGAATAAGTAGA+AGG - chr4.1:22716275-22716294 None:intergenic 15.0%
!! AATGACACATTTATTATTAT+AGG - chr4.1:22716336-22716355 None:intergenic 15.0%
!! AGATTAATCAAACATATATA+TGG - chr4.1:22715924-22715943 None:intergenic 15.0%
!! TAAATATTATTATATGCAGA+AGG + chr4.1:22717120-22717139 MS.gene26577:intron 15.0%
!!! AATTAATTTTTTATGTGATC+AGG + chr4.1:22716796-22716815 MS.gene26577:intron 15.0%
!! TGTTAGAATATTATGTAAAC+TGG - chr4.1:22716653-22716672 None:intergenic 20.0%
!!! CACATAAATATGTTTTTGAA+AGG + chr4.1:22716769-22716788 MS.gene26577:intron 20.0%
! AGTAACTAATATGGATAGAT+CGG + chr4.1:22716523-22716542 MS.gene26577:intron 25.0%
! ATTCATGAATTTACTTTACC+AGG + chr4.1:22717027-22717046 MS.gene26577:intron 25.0%
! GACACATTTATTATTATAGG+TGG - chr4.1:22716333-22716352 None:intergenic 25.0%
! GTCATGAAAACAAATTTCTA+TGG + chr4.1:22716881-22716900 MS.gene26577:CDS 25.0%
! TATTTGTGCTAAATGAATGA+AGG + chr4.1:22715959-22715978 MS.gene26577:intron 25.0%
! TTATCATTGTGAAACTAAGA+GGG - chr4.1:22716373-22716392 None:intergenic 25.0%
! TTCACAATGATAAACATGTT+TGG + chr4.1:22716380-22716399 MS.gene26577:intron 25.0%
! TTTACCAGGTTAATATAAGT+TGG + chr4.1:22717041-22717060 MS.gene26577:intron 25.0%
! TTTATCATTGTGAAACTAAG+AGG - chr4.1:22716374-22716393 None:intergenic 25.0%
!!! AAAGCGAAAAATATTGACTT+TGG + chr4.1:22716491-22716510 MS.gene26577:intron 25.0%
AATACATACGAGAATATCCA+AGG - chr4.1:22716247-22716266 None:intergenic 30.0%
ATGAAAATGTGTATAGTACC+TGG - chr4.1:22715872-22715891 None:intergenic 30.0%
CATAGTTCGAGTAACTAATA+TGG + chr4.1:22716514-22716533 MS.gene26577:intron 30.0%
CTTTGGTCAAAAAATTGTCT+AGG - chr4.1:22716716-22716735 None:intergenic 30.0%
TGGCATTAAATGTGTATAGT+AGG - chr4.1:22715904-22715923 None:intergenic 30.0%
! AAATGCATACTCAAAAGTGT+TGG + chr4.1:22717306-22717325 MS.gene26577:CDS 30.0%
! GTTTAGAATTAGCAACTTGT+TGG - chr4.1:22717552-22717571 None:intergenic 30.0%
! TTTCTAAGAGATGTGAGAAA+TGG + chr4.1:22717220-22717239 MS.gene26577:CDS 30.0%
!! AACCAAGATAGTGTTGATAA+TGG + chr4.1:22716617-22716636 MS.gene26577:intron 30.0%
!! ACCAAGATAGTGTTGATAAT+GGG + chr4.1:22716618-22716637 MS.gene26577:intron 30.0%
AACATTGAGAATACGTGTGA+TGG - chr4.1:22716969-22716988 None:intergenic 35.0%
AACCTCAATGACAAAAGGTA+AGG + chr4.1:22717417-22717436 MS.gene26577:CDS 35.0%
ACCTCAATGACAAAAGGTAA+GGG + chr4.1:22717418-22717437 MS.gene26577:CDS 35.0%
ACTAGAAAGTGAAGATGATG+AGG - chr4.1:22716156-22716175 None:intergenic 35.0%
ACTCAAACCTCAATGACAAA+AGG + chr4.1:22717412-22717431 MS.gene26577:CDS 35.0%
GATGGAAGAATTAGAAGAAG+AGG - chr4.1:22716951-22716970 None:intergenic 35.0%
GTGACCAACTTATATTAACC+TGG - chr4.1:22717048-22717067 None:intergenic 35.0%
TCAATGTTAGCTCAAGACTT+GGG + chr4.1:22716981-22717000 MS.gene26577:CDS 35.0%
TCCTCCTTCAAAGACAAATT+TGG + chr4.1:22716216-22716235 MS.gene26577:CDS 35.0%
TTGTGCTAAATGAATGAAGG+TGG + chr4.1:22715962-22715981 MS.gene26577:intron 35.0%
! AGCAAGAAGAATTTTCCAGT+GGG - chr4.1:22717511-22717530 None:intergenic 35.0%
! CAACACTATCTTGGTTTGAA+AGG - chr4.1:22716613-22716632 None:intergenic 35.0%
! GAAAAGAATTCTAGAGAGTG+AGG + chr4.1:22717159-22717178 MS.gene26577:CDS 35.0%
! TCCAAATTTGTCTTTGAAGG+AGG - chr4.1:22716220-22716239 None:intergenic 35.0%
! TTTTCATGACGTTTTCAGCA+TGG - chr4.1:22716871-22716890 None:intergenic 35.0%
!! AGAATTAGCAACTTGTTGGA+AGG - chr4.1:22717548-22717567 None:intergenic 35.0%
AAGAAGAGGAGCAACGAAAT+AGG - chr4.1:22716937-22716956 None:intergenic 40.0%
AATGGAACATGGAAGAGTCA+AGG + chr4.1:22717238-22717257 MS.gene26577:CDS 40.0%
ACATACGAGAATATCCAAGG+TGG - chr4.1:22716244-22716263 None:intergenic 40.0%
ACTGCTAGAGACAAGCAAAA+AGG + chr4.1:22716084-22716103 MS.gene26577:CDS 40.0%
AGAAGAGGAGCAACGAAATA+GGG - chr4.1:22716936-22716955 None:intergenic 40.0%
AGTGCAGGAGAAGGAAAATA+AGG - chr4.1:22715841-22715860 None:intergenic 40.0%
AGTGCCTCTATAAGCAACTT+AGG - chr4.1:22716908-22716927 None:intergenic 40.0%
ATGGAACATGGAAGAGTCAA+GGG + chr4.1:22717239-22717258 MS.gene26577:CDS 40.0%
CTCAATGTTAGCTCAAGACT+TGG + chr4.1:22716980-22716999 MS.gene26577:CDS 40.0%
CTTGAAGGAAGAAGAGATCA+AGG + chr4.1:22717183-22717202 MS.gene26577:CDS 40.0%
GAGATGTGAGAAATGGAACA+TGG + chr4.1:22717227-22717246 MS.gene26577:CDS 40.0%
GCATAGTCTGTCCAATATGA+TGG - chr4.1:22717268-22717287 None:intergenic 40.0%
GCCCATTATCAACACTATCT+TGG - chr4.1:22716622-22716641 None:intergenic 40.0%
GGAAGAAGAGATCAAGGAAA+TGG + chr4.1:22717189-22717208 MS.gene26577:CDS 40.0%
GGAGAAGGAAAATAAGGTTC+TGG - chr4.1:22715835-22715854 None:intergenic 40.0%
GGCTCCAAATTTGTCTTTGA+AGG - chr4.1:22716223-22716242 None:intergenic 40.0%
TAGAGACAAGCAAAAAGGAG+AGG + chr4.1:22716089-22716108 MS.gene26577:CDS 40.0%
TATGCAGAAGGTGACAAACT+TGG + chr4.1:22717132-22717151 MS.gene26577:intron 40.0%
TGCTAAATGAATGAAGGTGG+TGG + chr4.1:22715965-22715984 MS.gene26577:intron 40.0%
TGGTCCTAAGTTGCTTATAG+AGG + chr4.1:22716901-22716920 MS.gene26577:CDS 40.0%
TTATGGTCCATGTCTAGTCT+TGG - chr4.1:22716740-22716759 None:intergenic 40.0%
! AATCAGCATCTGAGACATCA+AGG - chr4.1:22716179-22716198 None:intergenic 40.0%
! GAGCAAGAAGAATTTTCCAG+TGG - chr4.1:22717512-22717531 None:intergenic 40.0%
! GTGAAACGTTTTGCAGAGTT+GGG + chr4.1:22716045-22716064 MS.gene26577:CDS 40.0%
! TGTGAAACGTTTTGCAGAGT+TGG + chr4.1:22716044-22716063 MS.gene26577:CDS 40.0%
!! ATTAGCAACTTGTTGGAAGG+TGG - chr4.1:22717545-22717564 None:intergenic 40.0%
ATTGAGGTTTGAGTGAAGCC+AGG - chr4.1:22717406-22717425 None:intergenic 45.0%
CCAAAGACCAAGACTAGACA+TGG + chr4.1:22716730-22716749 MS.gene26577:intron 45.0%
GAAGAGGAGCAACGAAATAG+GGG - chr4.1:22716935-22716954 None:intergenic 45.0%
GAAGATGATGAGGTGCAACA+AGG - chr4.1:22716146-22716165 None:intergenic 45.0%
TAGCTCAAGACTTGGGTCTT+TGG + chr4.1:22716988-22717007 MS.gene26577:CDS 45.0%
TCTAGAGAGTGAGGACTTGA+AGG + chr4.1:22717168-22717187 MS.gene26577:CDS 45.0%
TTGAGGTTTGAGTGAAGCCA+GGG - chr4.1:22717405-22717424 None:intergenic 45.0%
! AGAATTTTCCAGTGGGCAAG+TGG - chr4.1:22717504-22717523 None:intergenic 45.0%
! AGACAAATTTGGAGCCACCT+TGG + chr4.1:22716227-22716246 MS.gene26577:CDS 45.0%
! GCCCTTACCTTTTGTCATTG+AGG - chr4.1:22717422-22717441 None:intergenic 45.0%
! GTCTTTGAAGGAGGATGCAA+AGG - chr4.1:22716211-22716230 None:intergenic 45.0%
!! CCATGTCTAGTCTTGGTCTT+TGG - chr4.1:22716733-22716752 None:intergenic 45.0%
!! GAGAGCATTCGAGCACAATT+AGG + chr4.1:22716120-22716139 MS.gene26577:CDS 45.0%
!!! AGTGTGAACTGTTTTTGCCC+TGG + chr4.1:22717385-22717404 MS.gene26577:CDS 45.0%
!! ATTAATAATTCATATAAATT+AGG + chr4.1:22717343-22717362 MS.gene26577:CDS 5.0%
CATGGTCCACTGAATTCTCG+TGG - chr4.1:22716853-22716872 None:intergenic 50.0%
GAACATGGAAGAGTCAAGGG+TGG + chr4.1:22717242-22717261 MS.gene26577:CDS 50.0%
GCAAAAAGGAGAGGATGCTG+TGG + chr4.1:22716098-22716117 MS.gene26577:CDS 50.0%
GTCAAGGGTGGCCATCATAT+TGG + chr4.1:22717254-22717273 MS.gene26577:CDS 50.0%
GTGGTGACAGGAGGTAACAA+GGG + chr4.1:22716006-22716025 MS.gene26577:CDS 50.0%
GTTGAGCAAAGTGCAGGAGA+AGG - chr4.1:22715850-22715869 None:intergenic 50.0%
TACCTGGTTGAGCAAAGTGC+AGG - chr4.1:22715856-22715875 None:intergenic 50.0%
TGAGCTCCACGAGAATTCAG+TGG + chr4.1:22716844-22716863 MS.gene26577:CDS 50.0%
! ACAGGAGGTAACAAGGGCAT+TGG + chr4.1:22716012-22716031 MS.gene26577:CDS 50.0%
! TGAATGAAGGTGGTGGTCGA+AGG + chr4.1:22715972-22715991 MS.gene26577:intron 50.0%
!! CAGGAGGTAACAAGGGCATT+GGG + chr4.1:22716013-22716032 MS.gene26577:CDS 50.0%
CTCCTGCACTTTGCTCAACC+AGG + chr4.1:22715851-22715870 MS.gene26577:CDS 55.0%
GGTGGTGACAGGAGGTAACA+AGG + chr4.1:22716005-22716024 MS.gene26577:CDS 55.0%
TGGCGGAAGCAACACAAGGA+TGG - chr4.1:22717479-22717498 None:intergenic 55.0%
TTTCCAGTGGGCAAGTGGTG+TGG - chr4.1:22717499-22717518 None:intergenic 55.0%
! AAGGTGGTGGTCGAAGGAGA+CGG + chr4.1:22715978-22715997 MS.gene26577:intron 55.0%
! GGTGTGGCGGAAGCAACACA+AGG - chr4.1:22717483-22717502 None:intergenic 60.0%
CCAGTGGGCAAGTGGTGTGG+CGG - chr4.1:22717496-22717515 None:intergenic 65.0%
GTGGTCGAAGGAGACGGTGG+CGG + chr4.1:22715984-22716003 MS.gene26577:CDS 65.0%
GTGGTGGTCGAAGGAGACGG+TGG + chr4.1:22715981-22716000 MS.gene26577:CDS 65.0%
CCGCCACACCACTTGCCCAC+TGG + chr4.1:22717493-22717512 MS.gene26577:CDS 70.0%
! ACGGTGGCGGTGGTGACAGG+AGG + chr4.1:22715997-22716016 MS.gene26577:CDS 70.0%
! GAGACGGTGGCGGTGGTGAC+AGG + chr4.1:22715994-22716013 MS.gene26577:CDS 70.0%
! GTCGAAGGAGACGGTGGCGG+TGG + chr4.1:22715987-22716006 MS.gene26577:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 22715826 22717576 22715826 ID=MS.gene26577
chr4.1 mRNA 22715826 22717576 22715826 ID=MS.gene26577.t1;Parent=MS.gene26577
chr4.1 exon 22715826 22715872 22715826 ID=MS.gene26577.t1.exon1;Parent=MS.gene26577.t1
chr4.1 CDS 22715826 22715872 22715826 ID=cds.MS.gene26577.t1;Parent=MS.gene26577.t1
chr4.1 exon 22715981 22716257 22715981 ID=MS.gene26577.t1.exon2;Parent=MS.gene26577.t1
chr4.1 CDS 22715981 22716257 22715981 ID=cds.MS.gene26577.t1;Parent=MS.gene26577.t1
chr4.1 exon 22716818 22717012 22716818 ID=MS.gene26577.t1.exon3;Parent=MS.gene26577.t1
chr4.1 CDS 22716818 22717012 22716818 ID=cds.MS.gene26577.t1;Parent=MS.gene26577.t1
chr4.1 exon 22717139 22717576 22717139 ID=MS.gene26577.t1.exon4;Parent=MS.gene26577.t1
chr4.1 CDS 22717139 22717576 22717139 ID=cds.MS.gene26577.t1;Parent=MS.gene26577.t1
Gene Sequence

>MS.gene26577

ATGGAGCCAGAACCTTATTTTCCTTCTCCTGCACTTTGCTCAACCAGGTACTATACACATTTTCATCTTTTCAATCCTACTATACACATTTAATGCCATATATATGTTTGATTAATCTGCTTTTGCTTGTGTTTATTTGTGCTAAATGAATGAAGGTGGTGGTCGAAGGAGACGGTGGCGGTGGTGACAGGAGGTAACAAGGGCATTGGGTTTGCTCTTGTGAAACGTTTTGCAGAGTTGGGACTGAGTGTAGTGTTAACTGCTAGAGACAAGCAAAAAGGAGAGGATGCTGTGGAGAGCATTCGAGCACAATTAGGCCTTGTTGCACCTCATCATCTTCACTTTCTAGTCCTTGATGTCTCAGATGCTGATTCTATTAAAACCTTTGCATCCTCCTTCAAAGACAAATTTGGAGCCACCTTGGATATTCTCGTATGTATTAATTACCTTCTACTTATTCAATTTTATTTTTTGCATCATTTTGATTGATTTTATTCTGTCTCTCCACCTATAATAATAAATGTGTCATTTTATATATTAATTACCCTCTTAGTTTCACAATGATAAACATGTTTGGTTATTTCACAAATATATAAATAAAAGAGAGAAAATAATATTTTTAGTAAATTTATCAAGTATTTTGTATTCACAAGTATCATAAATGAAAAGCGAAAAATATTGACTTTGGCATAGTTCGAGTAACTAATATGGATAGATCGGATACAATTGAAAAAAATAATCAAGTTTACTCACATGACTCGAACTAAAAGACATGAGTTCACTTCCTTTCAAACCAAGATAGTGTTGATAATGGGCATAATACTCCAGTTTACATAATATTCTAACAAAATTATAAGATACAATAAGATATAAATACACACGTTAGACCTAGACAATTTTTTGACCAAAGACCAAGACTAGACATGGACCATAATTTTTTATTCACATAAATATGTTTTTGAAAGGATCTAATTAATTTTTTATGTGATCAGGTGAACAATGCAGCTGTATCATTCAATGAGCTCCACGAGAATTCAGTGGACCATGCTGAAAACGTCATGAAAACAAATTTCTATGGTCCTAAGTTGCTTATAGAGGCACTTCTTCCCCTATTTCGTTGCTCCTCTTCTTCTAATTCTTCCATCACACGTATTCTCAATGTTAGCTCAAGACTTGGGTCTTTGGATGTAAGTACCTAATAATTCATGAATTTACTTTACCAGGTTAATATAAGTTGGTCACATGCATAAAAAAAAAAAAAAAATTATATGGCATATTCTATGCGTGCTCAAACTAAATATTATTATATGCAGAAGGTGACAAACTTGGAAATGAAAAGAATTCTAGAGAGTGAGGACTTGAAGGAAGAAGAGATCAAGGAAATGGTGAGAAAATTTCTAAGAGATGTGAGAAATGGAACATGGAAGAGTCAAGGGTGGCCATCATATTGGACAGACTATGCAGTATCAAAATTGACTCTAAATGCATACTCAAAAGTGTTGGCAAAGAGATATAACATTAATAATTCATATAAATTAGGAAATACAACAAAGTTGATGAGTGTGAACTGTTTTTGCCCTGGCTTCACTCAAACCTCAATGACAAAAGGTAAGGGCACTCACACTGCTGATCAAGCTGCTTCTCTTGCTACCATCCTTGTGTTGCTTCCGCCACACCACTTGCCCACTGGAAAATTCTTCTTGCTCAGAAATAATACCACCTTCCAACAAGTTGCTAATTCTAAACTTTGA
Protein sequence

>MS.gene26577.t1

MEPEPYFPSPALCSTRWWSKETVAVVTGGNKGIGFALVKRFAELGLSVVLTARDKQKGEDAVESIRAQLGLVAPHHLHFLVLDVSDADSIKTFASSFKDKFGATLDILVNNAAVSFNELHENSVDHAENVMKTNFYGPKLLIEALLPLFRCSSSSNSSITRILNVSSRLGSLDKVTNLEMKRILESEDLKEEEIKEMVRKFLRDVRNGTWKSQGWPSYWTDYAVSKLTLNAYSKVLAKRYNINNSYKLGNTTKLMSVNCFCPGFTQTSMTKGKGTHTADQAASLATILVLLPPHHLPTGKFFLLRNNTTFQQVANSKL