AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47525

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47525.t1 MTR_4g087460 94.357 319 10 2 1 318 1 312 0.0 614
MS.gene47525.t1 MTR_3g098470 41.722 302 150 9 16 310 29 311 7.91e-74 230
MS.gene47525.t1 MTR_1g031180 40.000 300 158 8 16 310 34 316 9.13e-67 212
MS.gene47525.t1 MTR_8g096060 40.000 300 155 9 16 310 31 310 4.59e-66 210
MS.gene47525.t1 MTR_7g076660 39.667 300 146 8 24 310 9 286 1.08e-63 203
MS.gene47525.t1 MTR_7g076660 39.667 300 146 8 24 310 10 287 1.48e-63 202
MS.gene47525.t1 MTR_7g076655 38.667 300 149 8 24 310 9 286 1.13e-59 192
MS.gene47525.t1 MTR_8g031980 36.482 307 148 8 21 302 6 290 3.35e-58 189
MS.gene47525.t1 MTR_5g062280 38.111 307 144 7 19 302 4 287 3.36e-58 189
MS.gene47525.t1 MTR_7g076660 37.456 283 142 8 41 310 2 262 1.40e-54 179
MS.gene47525.t1 MTR_8g032000 36.513 304 146 8 24 302 9 290 5.63e-54 178
MS.gene47525.t1 MTR_8g031960 35.410 305 150 8 23 302 9 291 1.79e-53 177
MS.gene47525.t1 MTR_8g032010 34.304 309 156 8 20 303 5 291 2.39e-53 177
MS.gene47525.t1 MTR_8g031610 36.334 311 151 9 24 310 7 294 5.50e-53 176
MS.gene47525.t1 MTR_8g031910 37.217 309 152 10 19 310 4 287 1.34e-52 174
MS.gene47525.t1 MTR_8g031990 32.911 316 165 8 20 310 5 298 3.96e-52 174
MS.gene47525.t1 MTR_8g031940 35.144 313 155 9 24 310 9 299 3.07e-51 171
MS.gene47525.t1 MTR_8g031630 36.538 312 150 10 24 310 9 297 1.51e-50 169
MS.gene47525.t1 MTR_8g031600 37.134 307 146 9 22 303 7 291 2.54e-50 169
MS.gene47525.t1 MTR_8g032020 33.546 313 160 8 24 310 10 300 5.97e-50 168
MS.gene47525.t1 MTR_7g076665 35.548 301 150 8 24 302 9 287 1.95e-49 167
MS.gene47525.t1 MTR_8g031650 36.334 311 150 10 25 310 104 391 4.55e-49 168
MS.gene47525.t1 MTR_8g031650 32.500 120 66 4 169 285 1 108 3.57e-12 67.0
MS.gene47525.t1 MTR_8g031900 35.000 300 153 9 24 303 9 286 2.04e-48 164
MS.gene47525.t1 MTR_3g079320 36.943 314 147 10 18 302 7 298 4.39e-48 163
MS.gene47525.t1 MTR_8g031650 36.334 311 150 10 25 310 270 557 1.02e-47 168
MS.gene47525.t1 MTR_8g031650 37.282 287 134 9 24 285 9 274 9.00e-47 166
MS.gene47525.t1 MTR_7g106800 41.905 210 108 6 24 230 11 209 4.81e-41 144
MS.gene47525.t1 MTR_8g031850 32.028 281 149 8 24 303 9 248 1.36e-37 134
MS.gene47525.t1 MTR_5g083430 33.333 195 110 5 109 302 1 176 1.92e-28 108
MS.gene47525.t1 MTR_8g032030 38.122 181 78 5 21 176 6 177 8.55e-27 105
MS.gene47525.t1 MTR_6g041980 31.293 147 86 5 24 167 69 203 1.14e-13 70.5
MS.gene47525.t1 MTR_6g041980 31.507 146 85 5 25 167 70 203 2.91e-13 70.1
MS.gene47525.t1 MTR_4g105310 26.059 307 171 8 24 315 51 316 2.70e-12 67.0
MS.gene47525.t1 MTR_1g100315 29.493 217 121 5 24 240 32 216 1.18e-11 65.1
MS.gene47525.t1 MTR_4g105310 26.908 249 140 7 24 271 51 258 8.23e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47525.t1 AT3G59710 58.305 295 103 6 10 303 6 281 5.40e-115 334
MS.gene47525.t1 AT3G59710 58.537 287 100 5 17 303 24 291 7.03e-114 332
MS.gene47525.t1 AT3G59710 58.681 288 100 5 16 303 42 310 1.26e-113 332
MS.gene47525.t1 AT5G61830 43.522 301 149 8 16 310 31 316 3.73e-71 223
MS.gene47525.t1 AT5G61830 43.522 301 149 8 16 310 31 316 3.73e-71 223
MS.gene47525.t1 AT5G51030 40.000 300 158 7 16 310 32 314 5.65e-70 220
MS.gene47525.t1 AT3G61220 36.482 307 156 6 24 310 9 296 5.85e-57 186
MS.gene47525.t1 AT3G61220 36.482 307 156 6 24 310 16 303 8.55e-57 186
MS.gene47525.t1 AT3G61220 36.482 307 156 6 24 310 40 327 1.67e-56 186
MS.gene47525.t1 AT2G24190 34.675 323 164 8 8 310 6 301 1.37e-53 177
MS.gene47525.t1 AT1G01800 34.839 310 160 7 23 310 6 295 1.84e-53 177
MS.gene47525.t1 AT2G24190 34.853 307 161 6 24 310 9 296 6.49e-53 176
MS.gene47525.t1 AT1G01800 31.500 200 118 4 112 310 79 260 1.70e-28 110
MS.gene47525.t1 AT4G09750 33.088 136 86 3 25 160 47 177 4.99e-13 68.9

Find 70 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAATAATTCATATAAATT+AGG 0.194089 4.3:+25731322 MS.gene47525:CDS
TCCTCCTTCAAAGACAAATT+TGG 0.232306 4.3:+25730182 MS.gene47525:CDS
TAGCTCAAGACTTGGGTCTT+TGG 0.300326 4.3:+25730967 MS.gene47525:CDS
AGACTATGCAGTATCAAAAT+TGG 0.327322 4.3:+25731258 MS.gene47525:CDS
GTCAAGGGTGGCCATCATAT+TGG 0.371559 4.3:+25731233 MS.gene47525:CDS
AGTGCAGGAGAAGGAAAATA+AGG 0.378888 4.3:-25729802 None:intergenic
GTTTAGAATTAGCAACTTGT+TGG 0.384253 4.3:-25731528 None:intergenic
GAGACGGTGGCGGTGGTGAC+AGG 0.386563 4.3:+25729960 MS.gene47525:CDS
GGCTCCAAATTTGTCTTTGA+AGG 0.388365 4.3:-25730186 None:intergenic
TCTAGAGAGTGAGGACTTGA+AGG 0.406286 4.3:+25731147 MS.gene47525:CDS
TTTCTAAGAGATGTGAGAAA+TGG 0.407157 4.3:+25731199 MS.gene47525:CDS
GAGAGCATTCGAGCACAATT+AGG 0.410670 4.3:+25730086 MS.gene47525:CDS
GTCATGAAAACAAATTTCTA+TGG 0.424036 4.3:+25730860 MS.gene47525:CDS
CTCAATGTTAGCTCAAGACT+TGG 0.436992 4.3:+25730959 MS.gene47525:CDS
GGAAGAAGAGATCAAGGAAA+TGG 0.444648 4.3:+25731168 MS.gene47525:CDS
GGAGAAGGAAAATAAGGTTC+TGG 0.450582 4.3:-25729796 None:intergenic
CAGGAGGTAACAAGGGCATT+GGG 0.450651 4.3:+25729979 MS.gene47525:CDS
AAGAAGAGGAGCAACGAAAG+AGG 0.458793 4.3:-25730913 None:intergenic
CTCCTGCACTTTGCTCAACC+AGG 0.463222 4.3:+25729815 MS.gene47525:CDS
ACTGCTAGAGACAAGCAAAA+AGG 0.463408 4.3:+25730050 MS.gene47525:CDS
GTCGAAGGAGACGGTGGCGG+TGG 0.464837 4.3:+25729953 MS.gene47525:CDS
CCGCCACACCACTTGCCCAC+TGG 0.467591 4.3:+25731472 MS.gene47525:CDS
CTTGAAGGAAGAAGAGATCA+AGG 0.478401 4.3:+25731162 MS.gene47525:CDS
TATGCAGAAGGTGACAAACT+TGG 0.485647 4.3:+25731111 MS.gene47525:intron
GTCTTTGAAGGAGGATGCAA+AGG 0.489278 4.3:-25730174 None:intergenic
AGAAGAGGAGCAACGAAAGA+GGG 0.498513 4.3:-25730912 None:intergenic
ACTCAAACCTCAATGACAAA+AGG 0.505232 4.3:+25731391 MS.gene47525:CDS
ATTGAGGTTTGAGTGAAGCC+AGG 0.506250 4.3:-25731382 None:intergenic
AACCTCAATGACAAAAGGTA+AGG 0.507566 4.3:+25731396 MS.gene47525:CDS
GATGGAAGAATTAGAAGAAG+AGG 0.514502 4.3:-25730927 None:intergenic
GTGGTCGAAGGAGACGGTGG+CGG 0.516411 4.3:+25729950 MS.gene47525:CDS
AGACAAATTTGGAGCCACCT+TGG 0.518797 4.3:+25730193 MS.gene47525:CDS
GGTGGTGACAGGAGGTAACA+AGG 0.519926 4.3:+25729971 MS.gene47525:CDS
TGGTCCCAAGTTGCTTATAG+AGG 0.521817 4.3:+25730880 MS.gene47525:CDS
ACAGGAGGTAACAAGGGCAT+TGG 0.521906 4.3:+25729978 MS.gene47525:CDS
TCCAAATTTGTCTTTGAAGG+AGG 0.528089 4.3:-25730183 None:intergenic
TGAATGAAGGTGGTGGTCGA+AGG 0.543250 4.3:+25729938 MS.gene47525:intron
GCATAGTCTGTCCAATATGA+TGG 0.543911 4.3:-25731244 None:intergenic
AACATTGAGAATACGTGTGA+TGG 0.548769 4.3:-25730945 None:intergenic
AAATGCATACTCAAAAGTGT+TGG 0.552148 4.3:+25731285 MS.gene47525:CDS
AATCAGCATCTGAGACATCA+AGG 0.556283 4.3:-25730142 None:intergenic
AGAATTAGCAACTTGTTGGA+AGG 0.559112 4.3:-25731524 None:intergenic
TTTCCAGTGGGCAAGTGGTG+TGG 0.562735 4.3:-25731475 None:intergenic
TACCTGGTTGAGCAAAGTGC+AGG 0.563459 4.3:-25729817 None:intergenic
AAGTGCCTCTATAAGCAACT+TGG 0.563609 4.3:-25730885 None:intergenic
GAAAAGAATTCTAGAGAGTG+AGG 0.571043 4.3:+25731138 MS.gene47525:CDS
AGTGCCTCTATAAGCAACTT+GGG 0.573541 4.3:-25730884 None:intergenic
CCAGTGGGCAAGTGGTGTGG+CGG 0.576832 4.3:-25731472 None:intergenic
ATTAGCAACTTGTTGGAAGG+TGG 0.582292 4.3:-25731521 None:intergenic
GAAGAGGAGCAACGAAAGAG+GGG 0.583471 4.3:-25730911 None:intergenic
TCAATGTTAGCTCAAGACTT+GGG 0.584941 4.3:+25730960 MS.gene47525:CDS
ACGGTGGCGGTGGTGACAGG+AGG 0.588347 4.3:+25729963 MS.gene47525:CDS
ATGGAACATGGAAGAGTCAA+GGG 0.592718 4.3:+25731218 MS.gene47525:CDS
TTGAGGTTTGAGTGAAGCCA+GGG 0.599643 4.3:-25731381 None:intergenic
AATGGAACATGGAAGAGTCA+AGG 0.605080 4.3:+25731217 MS.gene47525:CDS
GTTGAGCAAAGTGCAGGAGA+AGG 0.605589 4.3:-25729811 None:intergenic
ACTAGAAAGTGAAGATGATG+AGG 0.607346 4.3:-25730119 None:intergenic
CATGGTCCACTGAATTCTCG+TGG 0.607377 4.3:-25730829 None:intergenic
GAGATGTGAGAAATGGAACA+TGG 0.612549 4.3:+25731206 MS.gene47525:CDS
GATACATACGAGAATATCCA+AGG 0.627537 4.3:-25730210 None:intergenic
ACCTCAATGACAAAAGGTAA+GGG 0.629438 4.3:+25731397 MS.gene47525:CDS
TGGCGGAAGCAACACAAGGA+TGG 0.632160 4.3:-25731455 None:intergenic
GTGGTGGTCGAAGGAGACGG+TGG 0.633275 4.3:+25729947 MS.gene47525:CDS
AAGGTGGTGGTCGAAGGAGA+CGG 0.651263 4.3:+25729944 MS.gene47525:intron
GTGGTGACAGGAGGTAACAA+GGG 0.653515 4.3:+25729972 MS.gene47525:CDS
GAAGATGATGAGGTGCAACA+AGG 0.666269 4.3:-25730109 None:intergenic
TGAGCTCCACGAGAATTCAG+TGG 0.667471 4.3:+25730823 MS.gene47525:CDS
GGTGTGGCGGAAGCAACACA+AGG 0.673747 4.3:-25731459 None:intergenic
GAACATGGAAGAGTCAAGGG+TGG 0.718135 4.3:+25731221 MS.gene47525:CDS
ACATACGAGAATATCCAAGG+TGG 0.779700 4.3:-25730207 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATAAAAATTATA+TGG + chr4.3:25731054-25731073 MS.gene47525:intron 0.0%
!! TTTATGTGAATAAAAAATTA+TGG - chr4.3:25730736-25730755 None:intergenic 10.0%
!! AAGTAAATTCATGAATTATT+AGG - chr4.3:25731002-25731021 None:intergenic 15.0%
!! AATAAAATTGAATAAGTAGA+AGG - chr4.3:25730241-25730260 None:intergenic 15.0%
!! AGATTAATCAAACATATATA+TGG - chr4.3:25729890-25729909 None:intergenic 15.0%
!! TAAATATTATTATATGCAGA+AGG + chr4.3:25731099-25731118 MS.gene47525:intron 15.0%
!!! AATTATTTTTTTATGTGATC+AGG + chr4.3:25730775-25730794 MS.gene47525:intron 15.0%
!! AATGACACATTTATTATTAG+AGG - chr4.3:25730302-25730321 None:intergenic 20.0%
!! TGTTAGAATATTATGTAAAC+TGG - chr4.3:25730632-25730651 None:intergenic 20.0%
!!! CACATAAATATGTTTTTGAA+AGG + chr4.3:25730748-25730767 MS.gene47525:intron 20.0%
! AGTAACTAATATGGATAGAT+CGG + chr4.3:25730489-25730508 MS.gene47525:intron 25.0%
! ATTCATGAATTTACTTTACC+AGG + chr4.3:25731006-25731025 MS.gene47525:intron 25.0%
! GTCATGAAAACAAATTTCTA+TGG + chr4.3:25730860-25730879 MS.gene47525:CDS 25.0%
! TATTTGTGCTAAATGAATGA+AGG + chr4.3:25729925-25729944 MS.gene47525:intron 25.0%
! TTATCATTGTGAAACTAAGA+GGG - chr4.3:25730339-25730358 None:intergenic 25.0%
! TTCACAATGATAAACATGTT+TGG + chr4.3:25730346-25730365 MS.gene47525:intron 25.0%
! TTTACCAGGTTAATATAAGT+TGG + chr4.3:25731020-25731039 MS.gene47525:intron 25.0%
! TTTATCATTGTGAAACTAAG+AGG - chr4.3:25730340-25730359 None:intergenic 25.0%
!!! AAAGCGAAAAATATTGACTT+TGG + chr4.3:25730457-25730476 MS.gene47525:intron 25.0%
AGACTATGCAGTATCAAAAT+TGG + chr4.3:25731258-25731277 MS.gene47525:CDS 30.0%
CATAGTTCGAGTAACTAATA+TGG + chr4.3:25730480-25730499 MS.gene47525:intron 30.0%
CTTTGGTCAAAAAATTGTCT+AGG - chr4.3:25730695-25730714 None:intergenic 30.0%
GAAAATGTGTATAGTATACC+TGG - chr4.3:25729836-25729855 None:intergenic 30.0%
GACACATTTATTATTAGAGG+TGG - chr4.3:25730299-25730318 None:intergenic 30.0%
TGGCATTAAATGTGTATAGT+AGG - chr4.3:25729870-25729889 None:intergenic 30.0%
! AAATGCATACTCAAAAGTGT+TGG + chr4.3:25731285-25731304 MS.gene47525:CDS 30.0%
! GTTTAGAATTAGCAACTTGT+TGG - chr4.3:25731531-25731550 None:intergenic 30.0%
! TTTCTAAGAGATGTGAGAAA+TGG + chr4.3:25731199-25731218 MS.gene47525:CDS 30.0%
!! AACCAAGATAGTGTTGATAA+TGG + chr4.3:25730596-25730615 MS.gene47525:intron 30.0%
!! ACCAAGATAGTGTTGATAAT+GGG + chr4.3:25730597-25730616 MS.gene47525:intron 30.0%
AACATTGAGAATACGTGTGA+TGG - chr4.3:25730948-25730967 None:intergenic 35.0%
AACCTCAATGACAAAAGGTA+AGG + chr4.3:25731396-25731415 MS.gene47525:CDS 35.0%
ACCTCAATGACAAAAGGTAA+GGG + chr4.3:25731397-25731416 MS.gene47525:CDS 35.0%
ACTAGAAAGTGAAGATGATG+AGG - chr4.3:25730122-25730141 None:intergenic 35.0%
ACTCAAACCTCAATGACAAA+AGG + chr4.3:25731391-25731410 MS.gene47525:CDS 35.0%
GATACATACGAGAATATCCA+AGG - chr4.3:25730213-25730232 None:intergenic 35.0%
GATGGAAGAATTAGAAGAAG+AGG - chr4.3:25730930-25730949 None:intergenic 35.0%
GTGACCAACTTATATTAACC+TGG - chr4.3:25731027-25731046 None:intergenic 35.0%
TCAATGTTAGCTCAAGACTT+GGG + chr4.3:25730960-25730979 MS.gene47525:CDS 35.0%
TCCTCCTTCAAAGACAAATT+TGG + chr4.3:25730182-25730201 MS.gene47525:CDS 35.0%
TTGTGCTAAATGAATGAAGG+TGG + chr4.3:25729928-25729947 MS.gene47525:intron 35.0%
! AGCAAGAAGAATTTTCCAGT+GGG - chr4.3:25731490-25731509 None:intergenic 35.0%
! CAACACTATCTTGGTTTGAA+AGG - chr4.3:25730592-25730611 None:intergenic 35.0%
! GAAAAGAATTCTAGAGAGTG+AGG + chr4.3:25731138-25731157 MS.gene47525:CDS 35.0%
! TCCAAATTTGTCTTTGAAGG+AGG - chr4.3:25730186-25730205 None:intergenic 35.0%
! TTTTCATGACGTTTTCAGCA+TGG - chr4.3:25730850-25730869 None:intergenic 35.0%
!! AGAATTAGCAACTTGTTGGA+AGG - chr4.3:25731527-25731546 None:intergenic 35.0%
AAGTGCCTCTATAAGCAACT+TGG - chr4.3:25730888-25730907 None:intergenic 40.0%
AATGGAACATGGAAGAGTCA+AGG + chr4.3:25731217-25731236 MS.gene47525:CDS 40.0%
ACATACGAGAATATCCAAGG+TGG - chr4.3:25730210-25730229 None:intergenic 40.0%
ACTGCTAGAGACAAGCAAAA+AGG + chr4.3:25730050-25730069 MS.gene47525:CDS 40.0%
AGTGCAGGAGAAGGAAAATA+AGG - chr4.3:25729805-25729824 None:intergenic 40.0%
AGTGCCTCTATAAGCAACTT+GGG - chr4.3:25730887-25730906 None:intergenic 40.0%
ATGGAACATGGAAGAGTCAA+GGG + chr4.3:25731218-25731237 MS.gene47525:CDS 40.0%
CTCAATGTTAGCTCAAGACT+TGG + chr4.3:25730959-25730978 MS.gene47525:CDS 40.0%
CTTGAAGGAAGAAGAGATCA+AGG + chr4.3:25731162-25731181 MS.gene47525:CDS 40.0%
GAGATGTGAGAAATGGAACA+TGG + chr4.3:25731206-25731225 MS.gene47525:CDS 40.0%
GCATAGTCTGTCCAATATGA+TGG - chr4.3:25731247-25731266 None:intergenic 40.0%
GCCCATTATCAACACTATCT+TGG - chr4.3:25730601-25730620 None:intergenic 40.0%
GGAAGAAGAGATCAAGGAAA+TGG + chr4.3:25731168-25731187 MS.gene47525:CDS 40.0%
GGAGAAGGAAAATAAGGTTC+TGG - chr4.3:25729799-25729818 None:intergenic 40.0%
GGCTCCAAATTTGTCTTTGA+AGG - chr4.3:25730189-25730208 None:intergenic 40.0%
TAGAGACAAGCAAAAAGGAG+AGG + chr4.3:25730055-25730074 MS.gene47525:CDS 40.0%
TATGCAGAAGGTGACAAACT+TGG + chr4.3:25731111-25731130 MS.gene47525:intron 40.0%
TGCTAAATGAATGAAGGTGG+TGG + chr4.3:25729931-25729950 MS.gene47525:intron 40.0%
TTATGGTCCATGTCTAGTCT+TGG - chr4.3:25730719-25730738 None:intergenic 40.0%
! AATCAGCATCTGAGACATCA+AGG - chr4.3:25730145-25730164 None:intergenic 40.0%
! GAGCAAGAAGAATTTTCCAG+TGG - chr4.3:25731491-25731510 None:intergenic 40.0%
! GTGAAACGTTTTGCAGAGTT+GGG + chr4.3:25730011-25730030 MS.gene47525:CDS 40.0%
! TGTGAAACGTTTTGCAGAGT+TGG + chr4.3:25730010-25730029 MS.gene47525:CDS 40.0%
!! ATTAGCAACTTGTTGGAAGG+TGG - chr4.3:25731524-25731543 None:intergenic 40.0%
AAGAAGAGGAGCAACGAAAG+AGG - chr4.3:25730916-25730935 None:intergenic 45.0%
AGAAGAGGAGCAACGAAAGA+GGG - chr4.3:25730915-25730934 None:intergenic 45.0%
ATTGAGGTTTGAGTGAAGCC+AGG - chr4.3:25731385-25731404 None:intergenic 45.0%
CCAAAGACCAAGACTAGACA+TGG + chr4.3:25730709-25730728 MS.gene47525:intron 45.0%
GAAGATGATGAGGTGCAACA+AGG - chr4.3:25730112-25730131 None:intergenic 45.0%
TAGCTCAAGACTTGGGTCTT+TGG + chr4.3:25730967-25730986 MS.gene47525:CDS 45.0%
TCTAGAGAGTGAGGACTTGA+AGG + chr4.3:25731147-25731166 MS.gene47525:CDS 45.0%
TGGTCCCAAGTTGCTTATAG+AGG + chr4.3:25730880-25730899 MS.gene47525:CDS 45.0%
TTGAGGTTTGAGTGAAGCCA+GGG - chr4.3:25731384-25731403 None:intergenic 45.0%
! AGAATTTTCCAGTGGGCAAG+TGG - chr4.3:25731483-25731502 None:intergenic 45.0%
! AGACAAATTTGGAGCCACCT+TGG + chr4.3:25730193-25730212 MS.gene47525:CDS 45.0%
! GCCCTTACCTTTTGTCATTG+AGG - chr4.3:25731401-25731420 None:intergenic 45.0%
! GTCTTTGAAGGAGGATGCAA+AGG - chr4.3:25730177-25730196 None:intergenic 45.0%
!! CCATGTCTAGTCTTGGTCTT+TGG - chr4.3:25730712-25730731 None:intergenic 45.0%
!! GAGAGCATTCGAGCACAATT+AGG + chr4.3:25730086-25730105 MS.gene47525:CDS 45.0%
!!! AGTGTGAACTGTTTTTGCCC+TGG + chr4.3:25731364-25731383 MS.gene47525:CDS 45.0%
!! ATTAATAATTCATATAAATT+AGG + chr4.3:25731322-25731341 MS.gene47525:CDS 5.0%
CATGGTCCACTGAATTCTCG+TGG - chr4.3:25730832-25730851 None:intergenic 50.0%
GAACATGGAAGAGTCAAGGG+TGG + chr4.3:25731221-25731240 MS.gene47525:CDS 50.0%
GAAGAGGAGCAACGAAAGAG+GGG - chr4.3:25730914-25730933 None:intergenic 50.0%
GCAAAAAGGAGAGGATGCTG+TGG + chr4.3:25730064-25730083 MS.gene47525:CDS 50.0%
GTCAAGGGTGGCCATCATAT+TGG + chr4.3:25731233-25731252 MS.gene47525:CDS 50.0%
GTGGTGACAGGAGGTAACAA+GGG + chr4.3:25729972-25729991 MS.gene47525:CDS 50.0%
GTTGAGCAAAGTGCAGGAGA+AGG - chr4.3:25729814-25729833 None:intergenic 50.0%
TACCTGGTTGAGCAAAGTGC+AGG - chr4.3:25729820-25729839 None:intergenic 50.0%
TGAGCTCCACGAGAATTCAG+TGG + chr4.3:25730823-25730842 MS.gene47525:CDS 50.0%
! ACAGGAGGTAACAAGGGCAT+TGG + chr4.3:25729978-25729997 MS.gene47525:CDS 50.0%
! TGAATGAAGGTGGTGGTCGA+AGG + chr4.3:25729938-25729957 MS.gene47525:intron 50.0%
!! CAGGAGGTAACAAGGGCATT+GGG + chr4.3:25729979-25729998 MS.gene47525:CDS 50.0%
CTCCTGCACTTTGCTCAACC+AGG + chr4.3:25729815-25729834 MS.gene47525:CDS 55.0%
GGTGGTGACAGGAGGTAACA+AGG + chr4.3:25729971-25729990 MS.gene47525:CDS 55.0%
TGGCGGAAGCAACACAAGGA+TGG - chr4.3:25731458-25731477 None:intergenic 55.0%
TTTCCAGTGGGCAAGTGGTG+TGG - chr4.3:25731478-25731497 None:intergenic 55.0%
! AAGGTGGTGGTCGAAGGAGA+CGG + chr4.3:25729944-25729963 MS.gene47525:intron 55.0%
! GGTGTGGCGGAAGCAACACA+AGG - chr4.3:25731462-25731481 None:intergenic 60.0%
CCAGTGGGCAAGTGGTGTGG+CGG - chr4.3:25731475-25731494 None:intergenic 65.0%
GTGGTCGAAGGAGACGGTGG+CGG + chr4.3:25729950-25729969 MS.gene47525:CDS 65.0%
GTGGTGGTCGAAGGAGACGG+TGG + chr4.3:25729947-25729966 MS.gene47525:CDS 65.0%
CCGCCACACCACTTGCCCAC+TGG + chr4.3:25731472-25731491 MS.gene47525:CDS 70.0%
! ACGGTGGCGGTGGTGACAGG+AGG + chr4.3:25729963-25729982 MS.gene47525:CDS 70.0%
! GAGACGGTGGCGGTGGTGAC+AGG + chr4.3:25729960-25729979 MS.gene47525:CDS 70.0%
! GTCGAAGGAGACGGTGGCGG+TGG + chr4.3:25729953-25729972 MS.gene47525:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 25729790 25731555 25729790 ID=MS.gene47525
chr4.3 mRNA 25729790 25731555 25729790 ID=MS.gene47525.t1;Parent=MS.gene47525
chr4.3 exon 25729790 25729836 25729790 ID=MS.gene47525.t1.exon1;Parent=MS.gene47525.t1
chr4.3 CDS 25729790 25729836 25729790 ID=cds.MS.gene47525.t1;Parent=MS.gene47525.t1
chr4.3 exon 25729947 25730223 25729947 ID=MS.gene47525.t1.exon2;Parent=MS.gene47525.t1
chr4.3 CDS 25729947 25730223 25729947 ID=cds.MS.gene47525.t1;Parent=MS.gene47525.t1
chr4.3 exon 25730797 25730991 25730797 ID=MS.gene47525.t1.exon3;Parent=MS.gene47525.t1
chr4.3 CDS 25730797 25730991 25730797 ID=cds.MS.gene47525.t1;Parent=MS.gene47525.t1
chr4.3 exon 25731118 25731555 25731118 ID=MS.gene47525.t1.exon4;Parent=MS.gene47525.t1
chr4.3 CDS 25731118 25731555 25731118 ID=cds.MS.gene47525.t1;Parent=MS.gene47525.t1
Gene Sequence

>MS.gene47525

ATGGAGCCAGAACCTTATTTTCCTTCTCCTGCACTTTGCTCAACCAGGTATACTATACACATTTTCATCTTTTCAATCCTACTATACACATTTAATGCCATATATATGTTTGATTAATCTGCTTTTGCTTGTGTTTATTTGTGCTAAATGAATGAAGGTGGTGGTCGAAGGAGACGGTGGCGGTGGTGACAGGAGGTAACAAGGGCATTGGGTTTGCTCTTGTGAAACGTTTTGCAGAGTTGGGACTGAGTGTAGTGTTAACTGCTAGAGACAAGCAAAAAGGAGAGGATGCTGTGGAGAGCATTCGAGCACAATTAGGCCTTGTTGCACCTCATCATCTTCACTTTCTAGTCCTTGATGTCTCAGATGCTGATTCTATTAAAACCTTTGCATCCTCCTTCAAAGACAAATTTGGAGCCACCTTGGATATTCTCGTATGTATCAATTACCTTCTACTTATTCAATTTTATTTTTTACATCATTTTGATTGATTTTATTCTGTCTCTCCACCTCTAATAATAAATGTGTCATTTTATATATTAATTACCCTCTTAGTTTCACAATGATAAACATGTTTGGTTATTTCACAAATATATAAATAAAAGAGAGAAAATAATATTTTTAGTAAATTTATCAAGTATTTTGTATTCACAAGTATCATAAATGAAAAGCGAAAAATATTGACTTTGGCATAGTTCGAGTAACTAATATGGATAGATCGGATACAATTGAAAAAAATAATCAAGTTTACTCACATGACTCGAACTTGAAACTTTATTTAAAAGACATGAGTTCACTTCCTTTCAAACCAAGATAGTGTTGATAATGGGCATAATACTCCAGTTTACATAATATTCTAACAAAATTATAAGATACAATAAGATATAAATACACACGTTAGACCTAGACAATTTTTTGACCAAAGACCAAGACTAGACATGGACCATAATTTTTTATTCACATAAATATGTTTTTGAAAGGATCTAATTATTTTTTTATGTGATCAGGTGAACAATGCAGCTGTATCATTCAATGAGCTCCACGAGAATTCAGTGGACCATGCTGAAAACGTCATGAAAACAAATTTCTATGGTCCCAAGTTGCTTATAGAGGCACTTCTTCCCCTCTTTCGTTGCTCCTCTTCTTCTAATTCTTCCATCACACGTATTCTCAATGTTAGCTCAAGACTTGGGTCTTTGGATGTAAGTACCTAATAATTCATGAATTTACTTTACCAGGTTAATATAAGTTGGTCACATGCATAAAAAAAAAATAAAAATTATATGGCATATTCTATGCGTGCTCAAACTAAATATTATTATATGCAGAAGGTGACAAACTTGGAAATGAAAAGAATTCTAGAGAGTGAGGACTTGAAGGAAGAAGAGATCAAGGAAATGGTGAGAAAATTTCTAAGAGATGTGAGAAATGGAACATGGAAGAGTCAAGGGTGGCCATCATATTGGACAGACTATGCAGTATCAAAATTGGCTCTAAATGCATACTCAAAAGTGTTGGCTAAGAGATATAACATTAATAATTCATATAAATTAGGAAATACAACAAAGTTGATGAGTGTGAACTGTTTTTGCCCTGGCTTCACTCAAACCTCAATGACAAAAGGTAAGGGCACTCACACTGCTGATCAAGCTGCTTCTCTTGCTACCATCCTTGTGTTGCTTCCGCCACACCACTTGCCCACTGGAAAATTCTTCTTGCTCAGAAATAATACCACCTTCCAACAAGTTGCTAATTCTAAACTTTGA
Protein sequence

>MS.gene47525.t1

MEPEPYFPSPALCSTRWWSKETVAVVTGGNKGIGFALVKRFAELGLSVVLTARDKQKGEDAVESIRAQLGLVAPHHLHFLVLDVSDADSIKTFASSFKDKFGATLDILVNNAAVSFNELHENSVDHAENVMKTNFYGPKLLIEALLPLFRCSSSSNSSITRILNVSSRLGSLDKVTNLEMKRILESEDLKEEEIKEMVRKFLRDVRNGTWKSQGWPSYWTDYAVSKLALNAYSKVLAKRYNINNSYKLGNTTKLMSVNCFCPGFTQTSMTKGKGTHTADQAASLATILVLLPPHHLPTGKFFLLRNNTTFQQVANSKL