AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003314.t1 MTR_2g030390 96.965 659 19 1 1 659 1 658 0.0 1260
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003314.t1 AT3G24080 45.250 579 268 13 5 555 8 565 6.86e-109 342
MS.gene003314.t1 AT3G24080 45.250 579 268 13 5 555 8 565 7.24e-109 342
MS.gene003314.t1 AT3G24080 45.250 579 268 13 5 555 8 565 7.64e-109 342
MS.gene003314.t1 AT3G24080 45.250 579 268 13 5 555 8 565 9.49e-109 342

Find 117 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCTGTCTTCTCCTTTA+AGG 0.067645 2.4:+59294037 None:intergenic
ATTGAGGAGGGACCAGATTT+TGG 0.223663 2.4:-59294197 MS.gene003314:CDS
TAAGCTTCAATTCCTCTTCT+TGG 0.247682 2.4:+59293013 None:intergenic
ATTCTTTGATGCTTTGATTA+AGG 0.251168 2.4:-59294366 MS.gene003314:CDS
ATCATTCTGTCATATTCCTC+TGG 0.284675 2.4:+59293528 None:intergenic
CCACATTCATCCCAATCTTT+AGG 0.290694 2.4:+59293384 None:intergenic
GATCCTTCGTGCAGCAAGTT+TGG 0.295537 2.4:-59292950 MS.gene003314:CDS
AAAAGCAAACACAGCAAGTT+TGG 0.300133 2.4:-59292836 MS.gene003314:CDS
GAAGCGTAAAACTGCACTTT+TGG 0.321590 2.4:-59293229 MS.gene003314:CDS
TGAGGAATTTGAGGAGAAAC+TGG 0.339212 2.4:-59293994 MS.gene003314:CDS
TACTTGGGCTTCCTAAAGAT+TGG 0.346122 2.4:-59293395 MS.gene003314:CDS
CACTTTCATCTTCACTACTA+TGG 0.346312 2.4:+59294288 None:intergenic
TGAGATGGAAATTGAGAGGC+AGG 0.355422 2.4:-59293841 MS.gene003314:CDS
AAGCCAAGAGAAGGGAAAAC+AGG 0.359507 2.4:-59292705 MS.gene003314:CDS
ATCCTTCGTGCAGCAAGTTT+GGG 0.366521 2.4:-59292949 MS.gene003314:CDS
AAAACAAAACCTAACAGATT+TGG 0.379299 2.4:-59293114 MS.gene003314:CDS
GAGTGAAGATGAGGAATTTG+AGG 0.381021 2.4:-59294003 MS.gene003314:CDS
TGCTCGTAAGGTGGAGGGTT+CGG 0.395725 2.4:-59293766 MS.gene003314:CDS
GACTTGCTAGAATCAGCCTT+GGG 0.399472 2.4:+59292793 None:intergenic
TCCTCTTTATCACCAAAATC+TGG 0.405490 2.4:+59294185 None:intergenic
GTATCCAGGCCAAATCTGTT+AGG 0.416690 2.4:+59293105 None:intergenic
TAGATTATGAGGACACAGTT+GGG 0.421516 2.4:-59293167 MS.gene003314:CDS
ACTTGGGCTTCCTAAAGATT+GGG 0.423740 2.4:-59293394 MS.gene003314:CDS
TCATCGATCAATAGTATCTC+TGG 0.430675 2.4:+59293081 None:intergenic
GAGTCGAGACTTAAAGCATA+TGG 0.437812 2.4:-59292664 MS.gene003314:CDS
AGCAGCAGAAAAGGATGGCT+TGG 0.438918 2.4:-59294078 MS.gene003314:CDS
TTCAATTCCTCTTCTTGGTA+AGG 0.450588 2.4:+59293018 None:intergenic
CTTCCGACTACTACCTTCCT+CGG 0.452952 2.4:+59293254 None:intergenic
GATACTATTGATCGATGATA+AGG 0.458126 2.4:-59293076 MS.gene003314:CDS
GAGAGAGGCAATACGACAAA+AGG 0.459308 2.4:-59293694 MS.gene003314:CDS
AGATCCTGTTCTTAAGCAAA+AGG 0.465168 2.4:-59294333 MS.gene003314:CDS
GTCTACTGCAGAATTAGAAA+AGG 0.471726 2.4:-59293559 MS.gene003314:CDS
CTAATTCTGCAGTAGACAAA+GGG 0.472024 2.4:+59293565 None:intergenic
TCATTCTGTCATATTCCTCT+GGG 0.472452 2.4:+59293529 None:intergenic
TGAGCAAGAGGAGTTGAAAC+AGG 0.482808 2.4:-59294111 MS.gene003314:CDS
AATGAGAAAGGTATATCAAA+TGG 0.485679 2.4:-59294155 MS.gene003314:CDS
AAATCCAGGGGATAGAGTGC+TGG 0.488077 2.4:-59293793 MS.gene003314:CDS
TACTAGTGATGATGATCTCA+TGG 0.493040 2.4:-59293304 MS.gene003314:CDS
TTTCAACTCTAAGAGTGATA+AGG 0.498953 2.4:-59294390 MS.gene003314:CDS
GATGAATGTGGATCTGATGG+TGG 0.500163 2.4:-59293372 MS.gene003314:CDS
GGGTCATGCTCGTAAGGTGG+AGG 0.503285 2.4:-59293772 MS.gene003314:CDS
TGATGAGAAGAGTAAAGATA+AGG 0.513195 2.4:-59294264 MS.gene003314:CDS
GCAGCAGAAAAGGATGGCTT+GGG 0.515479 2.4:-59294077 MS.gene003314:CDS
AGAGATTTCAGAAGATGAGA+TGG 0.521290 2.4:-59293856 MS.gene003314:CDS
AAAACCTAACAGATTTGGCC+TGG 0.522228 2.4:-59293109 MS.gene003314:CDS
GAGGATGAAACAAATGAGAA+AGG 0.523006 2.4:-59294167 MS.gene003314:CDS
CTGAAAGCAGCAGAAAAGGA+TGG 0.523891 2.4:-59294083 MS.gene003314:CDS
CTCACCTTAAAGGAGAAGAC+AGG 0.529520 2.4:-59294041 MS.gene003314:CDS
CAAGGTAGAGAGTGAAGATG+AGG 0.530933 2.4:-59294012 MS.gene003314:CDS
GACTTATGCTGATGAGCAAG+AGG 0.531868 2.4:-59294123 MS.gene003314:CDS
AAAGAGCAAAGAGGAGAGAG+AGG 0.532174 2.4:-59293709 MS.gene003314:CDS
ATGAATGTGGATCTGATGGT+GGG 0.532471 2.4:-59293371 MS.gene003314:CDS
GAAAGCAAGACAAATGATGA+TGG 0.538249 2.4:-59293205 MS.gene003314:CDS
ACACAGAAGAATCAAAATCA+AGG 0.538980 2.4:-59292729 MS.gene003314:CDS
AGTGCTGGGTCATGCTCGTA+AGG 0.540823 2.4:-59293778 MS.gene003314:CDS
AGATTATGAGGACACAGTTG+GGG 0.541091 2.4:-59293166 MS.gene003314:CDS
TAGTGAGGGTGAAGAAGACA+TGG 0.541771 2.4:-59293448 MS.gene003314:CDS
TCTAATTCTGCAGTAGACAA+AGG 0.542118 2.4:+59293564 None:intergenic
TTAGATTATGAGGACACAGT+TGG 0.543563 2.4:-59293168 MS.gene003314:CDS
GTTAACTTCAAACAATGTTG+TGG 0.543762 2.4:-59292866 MS.gene003314:CDS
TGACCCAGCACTCTATCCCC+TGG 0.546475 2.4:+59293789 None:intergenic
ATAGTATCTCTGGAGTATCC+AGG 0.549189 2.4:+59293091 None:intergenic
TGACTTGCTAGAATCAGCCT+TGG 0.550613 2.4:+59292792 None:intergenic
AAAGATACATGCTGAAGATG+AGG 0.550832 2.4:-59292981 MS.gene003314:CDS
GGCAAAGCATTTGATTGAGG+AGG 0.551984 2.4:-59294210 MS.gene003314:CDS
CTCATCAAACAGTTGTGTTG+TGG 0.554487 2.4:-59292767 MS.gene003314:CDS
ACTTGCTCCTTACCAAGAAG+AGG 0.555311 2.4:-59293025 MS.gene003314:CDS
ACTATTTCAAGAACAAAATG+TGG 0.558440 2.4:-59293926 MS.gene003314:CDS
AGGAAGGTAGTAGTCGGAAG+AGG 0.560258 2.4:-59293251 MS.gene003314:CDS
TTCCGACTACTACCTTCCTC+GGG 0.560833 2.4:+59293255 None:intergenic
AATCAAGGAAAGCCAAGAGA+AGG 0.564095 2.4:-59292714 MS.gene003314:CDS
TATTGGTTCACCTTCACACT+CGG 0.566868 2.4:-59294462 MS.gene003314:CDS
AATCCAGGGGATAGAGTGCT+GGG 0.568166 2.4:-59293792 MS.gene003314:CDS
AAAGGATGGCTTGGGAGATG+AGG 0.568697 2.4:-59294069 MS.gene003314:CDS
ATCAAGGAAAGCCAAGAGAA+GGG 0.568922 2.4:-59292713 MS.gene003314:CDS
TGGGATGAATGTGGATCTGA+TGG 0.570198 2.4:-59293375 MS.gene003314:CDS
GGCAATACGACAAAAGGAAA+GGG 0.576585 2.4:-59293688 MS.gene003314:CDS
GCAAAGCATTTGATTGAGGA+GGG 0.577160 2.4:-59294209 MS.gene003314:CDS
AGGCAATACGACAAAAGGAA+AGG 0.579482 2.4:-59293689 MS.gene003314:CDS
CATGCTGAAGATGAGGGCTA+AGG 0.580350 2.4:-59292974 MS.gene003314:CDS
AAGATACATGCTGAAGATGA+GGG 0.584857 2.4:-59292980 MS.gene003314:CDS
ACGACAAAAGGAAAGGGATG+AGG 0.586281 2.4:-59293682 MS.gene003314:CDS
TTATGAGGACACAGTTGGGG+GGG 0.587460 2.4:-59293163 MS.gene003314:CDS
CCTAAAGATTGGGATGAATG+TGG 0.587753 2.4:-59293384 MS.gene003314:CDS
AAAGAGGACATTCCCGAGGA+AGG 0.588781 2.4:-59293267 MS.gene003314:CDS
GTTTGAGCATAACAAGAGGC+GGG 0.590327 2.4:-59294525 MS.gene003314:CDS
TATGCTTTAAGTCTCGACTC+TGG 0.591074 2.4:+59292667 None:intergenic
AAAAGATAAAGAAGATTGCA+GGG 0.591451 2.4:-59293608 MS.gene003314:CDS
GCTGGGTCATGCTCGTAAGG+TGG 0.591483 2.4:-59293775 MS.gene003314:CDS
AACTTCAAACAATGTTGTGG+AGG 0.597494 2.4:-59292863 MS.gene003314:CDS
GAGAAAGGTATATCAAATGG+TGG 0.598159 2.4:-59294152 MS.gene003314:CDS
GGAGAAGACAGGTGAAGACA+AGG 0.599497 2.4:-59294030 MS.gene003314:CDS
AGCCAAGAGAAGGGAAAACA+GGG 0.602506 2.4:-59292704 MS.gene003314:CDS
GGTCATGCTCGTAAGGTGGA+GGG 0.605836 2.4:-59293771 MS.gene003314:CDS
GGTGGCAAAGCATTTGATTG+AGG 0.605973 2.4:-59294213 MS.gene003314:CDS
CACAACACAACTGTTTGATG+AGG 0.608834 2.4:+59292768 None:intergenic
GATTATGAGGACACAGTTGG+GGG 0.611884 2.4:-59293165 MS.gene003314:CDS
GATGCAAGAGATACAAGAGA+AGG 0.616907 2.4:-59293634 MS.gene003314:CDS
AGTGATGATGATCTCATGGA+AGG 0.617533 2.4:-59293300 MS.gene003314:CDS
TCATCATCTTCCGAGTGTGA+AGG 0.624572 2.4:+59294452 None:intergenic
TTCCCGAGGAAGGTAGTAGT+CGG 0.625706 2.4:-59293257 MS.gene003314:CDS
GGTTTGAGCATAACAAGAGG+CGG 0.627786 2.4:-59294526 MS.gene003314:CDS
AAGATGAGATGGAAATTGAG+AGG 0.629773 2.4:-59293845 MS.gene003314:CDS
GAAGAGAAATAAGAAGCCCA+AGG 0.630672 2.4:-59292809 MS.gene003314:CDS
ATTATGAGGACACAGTTGGG+GGG 0.631668 2.4:-59293164 MS.gene003314:CDS
TGCGAAGTATTATAACGAAG+AGG 0.635980 2.4:-59293490 MS.gene003314:CDS
CTCAAATATCAAGATAGACG+AGG 0.643202 2.4:-59294561 MS.gene003314:CDS
TACCCAAACTTGCTGCACGA+AGG 0.643872 2.4:+59292947 None:intergenic
ATCAAACAGTTGTGTTGTGG+AGG 0.648436 2.4:-59292764 MS.gene003314:CDS
AGAGCAGAGAAAGAGCAAAG+AGG 0.649070 2.4:-59293718 MS.gene003314:CDS
AGAAAAGGAGTTTGACCCAG+AGG 0.654123 2.4:-59293544 MS.gene003314:CDS
TAGACGAGGAATATGCTCGT+AGG 0.664106 2.4:-59294547 MS.gene003314:CDS
CTATTTCAAGAACAAAATGT+GGG 0.673159 2.4:-59293925 MS.gene003314:CDS
TGAGCATAACAAGAGGCGGG+AGG 0.702904 2.4:-59294522 MS.gene003314:CDS
GTAGGTTTGAGCATAACAAG+AGG 0.707686 2.4:-59294529 MS.gene003314:CDS
GTAAGGTGGAGGGTTCGGTG+AGG 0.731572 2.4:-59293761 MS.gene003314:CDS
AATGTGGGTAGACAAGAATG+CGG 0.745968 2.4:-59293910 MS.gene003314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATAAATTAGATTATG+AGG - chr2.4:59294081-59294100 MS.gene003314:CDS 15.0%
!! ATGAAGAATTGAAAAAGAAA+GGG - chr2.4:59292772-59292791 MS.gene003314:CDS 20.0%
!! TATGAAGAATTGAAAAAGAA+AGG - chr2.4:59292771-59292790 MS.gene003314:CDS 20.0%
! AAAAAGAGGATGATTTACTT+GGG - chr2.4:59293849-59293868 MS.gene003314:CDS 25.0%
! AAAAAGATAAAGAAGATTGC+AGG - chr2.4:59293650-59293669 MS.gene003314:CDS 25.0%
! AAAACAAAACCTAACAGATT+TGG - chr2.4:59294145-59294164 MS.gene003314:CDS 25.0%
! AAAAGATAAAGAAGATTGCA+GGG - chr2.4:59293651-59293670 MS.gene003314:CDS 25.0%
! ACTATTTCAAGAACAAAATG+TGG - chr2.4:59293333-59293352 MS.gene003314:CDS 25.0%
! ATTCTTTGATGCTTTGATTA+AGG - chr2.4:59292893-59292912 MS.gene003314:CDS 25.0%
! CTATTTCAAGAACAAAATGT+GGG - chr2.4:59293334-59293353 MS.gene003314:CDS 25.0%
! TAAGAAGAAAAAGAGGTTAA+AGG - chr2.4:59294333-59294352 MS.gene003314:CDS 25.0%
! TGAAGAATTGAAAAAGAAAG+GGG - chr2.4:59292773-59292792 MS.gene003314:CDS 25.0%
!! AATGAGAAAGGTATATCAAA+TGG - chr2.4:59293104-59293123 MS.gene003314:CDS 25.0%
!! TTTTCAATTCTTCATAACGT+TGG + chr2.4:59292767-59292786 None:intergenic 25.0%
!!! AAATCTGTTAGGTTTTGTTT+TGG + chr2.4:59294146-59294165 None:intergenic 25.0%
!!! GAAAAAGTCAATGTTTTTGA+AGG - chr2.4:59293019-59293038 MS.gene003314:CDS 25.0%
ACACAGAAGAATCAAAATCA+AGG - chr2.4:59294530-59294549 MS.gene003314:CDS 30.0%
GAAAAAGAGGATGATTTACT+TGG - chr2.4:59293848-59293867 MS.gene003314:CDS 30.0%
GATACTATTGATCGATGATA+AGG - chr2.4:59294183-59294202 MS.gene003314:CDS 30.0%
GTTAACTTCAAACAATGTTG+TGG - chr2.4:59294393-59294412 MS.gene003314:CDS 30.0%
TGATGAGAAGAGTAAAGATA+AGG - chr2.4:59292995-59293014 MS.gene003314:CDS 30.0%
TTGAAAAAGAAAGGGGTTAT+TGG - chr2.4:59292780-59292799 MS.gene003314:CDS 30.0%
! ATGACTCAGAACATTTTTGA+TGG - chr2.4:59292651-59292670 MS.gene003314:CDS 30.0%
! TTTCAACTCTAAGAGTGATA+AGG - chr2.4:59292869-59292888 MS.gene003314:CDS 30.0%
!! TTAGTTTTCAGGAAAATCCA+GGG - chr2.4:59293453-59293472 MS.gene003314:CDS 30.0%
!!! AGAGTATGAAGTTAGTTTTC+AGG - chr2.4:59293442-59293461 MS.gene003314:CDS 30.0%
!!! TCATCCTCTTTTTCAAAATC+TGG + chr2.4:59293842-59293861 None:intergenic 30.0%
AAAAGCAAACACAGCAAGTT+TGG - chr2.4:59294423-59294442 MS.gene003314:CDS 35.0%
AAAGATACATGCTGAAGATG+AGG - chr2.4:59294278-59294297 MS.gene003314:CDS 35.0%
AACTTCAAACAATGTTGTGG+AGG - chr2.4:59294396-59294415 MS.gene003314:CDS 35.0%
AAGATACATGCTGAAGATGA+GGG - chr2.4:59294279-59294298 MS.gene003314:CDS 35.0%
AAGATGAGATGGAAATTGAG+AGG - chr2.4:59293414-59293433 MS.gene003314:CDS 35.0%
AGAGATTTCAGAAGATGAGA+TGG - chr2.4:59293403-59293422 MS.gene003314:CDS 35.0%
AGATCCTGTTCTTAAGCAAA+AGG - chr2.4:59292926-59292945 MS.gene003314:CDS 35.0%
ATCATTCTGTCATATTCCTC+TGG + chr2.4:59293734-59293753 None:intergenic 35.0%
CACTTTCATCTTCACTACTA+TGG + chr2.4:59292974-59292993 None:intergenic 35.0%
CTAATTCTGCAGTAGACAAA+GGG + chr2.4:59293697-59293716 None:intergenic 35.0%
CTCAAATATCAAGATAGACG+AGG - chr2.4:59292698-59292717 MS.gene003314:CDS 35.0%
GAAAGCAAGACAAATGATGA+TGG - chr2.4:59294054-59294073 MS.gene003314:CDS 35.0%
GAGGATGAAACAAATGAGAA+AGG - chr2.4:59293092-59293111 MS.gene003314:CDS 35.0%
GTCTACTGCAGAATTAGAAA+AGG - chr2.4:59293700-59293719 MS.gene003314:CDS 35.0%
GTTTGGGTAAGAAGAAAAAG+AGG - chr2.4:59294326-59294345 MS.gene003314:CDS 35.0%
TAAGCTTCAATTCCTCTTCT+TGG + chr2.4:59294249-59294268 None:intergenic 35.0%
TAGATTATGAGGACACAGTT+GGG - chr2.4:59294092-59294111 MS.gene003314:CDS 35.0%
TCATCGATCAATAGTATCTC+TGG + chr2.4:59294181-59294200 None:intergenic 35.0%
TCATTCTGTCATATTCCTCT+GGG + chr2.4:59293733-59293752 None:intergenic 35.0%
TCCTCTTTATCACCAAAATC+TGG + chr2.4:59293077-59293096 None:intergenic 35.0%
TCTAATTCTGCAGTAGACAA+AGG + chr2.4:59293698-59293717 None:intergenic 35.0%
TGAGGAAAAAGACAAACTCA+AGG - chr2.4:59293516-59293535 MS.gene003314:CDS 35.0%
TTAGATTATGAGGACACAGT+TGG - chr2.4:59294091-59294110 MS.gene003314:CDS 35.0%
TTCAATTCCTCTTCTTGGTA+AGG + chr2.4:59294244-59294263 None:intergenic 35.0%
! AATGCGGAGATTTTGAATGT+TGG - chr2.4:59293365-59293384 MS.gene003314:CDS 35.0%
! ACATCCTTTTGCTTAAGAAC+AGG + chr2.4:59292933-59292952 None:intergenic 35.0%
! GAGAAAGGTATATCAAATGG+TGG - chr2.4:59293107-59293126 MS.gene003314:CDS 35.0%
! TACTAGTGATGATGATCTCA+TGG - chr2.4:59293955-59293974 MS.gene003314:CDS 35.0%
! TGAGGATTTTCTCACCTTAA+AGG - chr2.4:59293208-59293227 MS.gene003314:CDS 35.0%
! TGCGAAGTATTATAACGAAG+AGG - chr2.4:59293769-59293788 MS.gene003314:CDS 35.0%
!! AATGTTTTTGAAGGATGTGG+TGG - chr2.4:59293028-59293047 MS.gene003314:CDS 35.0%
!! ACCAGATTTTGGTGATAAAG+AGG - chr2.4:59293073-59293092 MS.gene003314:CDS 35.0%
!! GAAGCCAGATTTTGAAAAAG+AGG - chr2.4:59293835-59293854 MS.gene003314:CDS 35.0%
!! GTCAATGTTTTTGAAGGATG+TGG - chr2.4:59293025-59293044 MS.gene003314:CDS 35.0%
!! TAGTTTTCAGGAAAATCCAG+GGG - chr2.4:59293454-59293473 MS.gene003314:CDS 35.0%
!! TTTTCTGAAAGCAGCAGAAA+AGG - chr2.4:59293172-59293191 MS.gene003314:CDS 35.0%
!!! GTAAGGAGCAAGTTTTTTCA+AGG + chr2.4:59294227-59294246 None:intergenic 35.0%
!!! GTTAGTTTTCAGGAAAATCC+AGG - chr2.4:59293452-59293471 MS.gene003314:CDS 35.0%
AAAACCTAACAGATTTGGCC+TGG - chr2.4:59294150-59294169 MS.gene003314:CDS 40.0%
AATCAAGGAAAGCCAAGAGA+AGG - chr2.4:59294545-59294564 MS.gene003314:CDS 40.0%
AATGTGGGTAGACAAGAATG+CGG - chr2.4:59293349-59293368 MS.gene003314:CDS 40.0%
ACTTGGGCTTCCTAAAGATT+GGG - chr2.4:59293865-59293884 MS.gene003314:CDS 40.0%
AGATTATGAGGACACAGTTG+GGG - chr2.4:59294093-59294112 MS.gene003314:CDS 40.0%
AGGCAATACGACAAAAGGAA+AGG - chr2.4:59293570-59293589 MS.gene003314:CDS 40.0%
ATCAAACAGTTGTGTTGTGG+AGG - chr2.4:59294495-59294514 MS.gene003314:CDS 40.0%
ATCAAGGAAAGCCAAGAGAA+GGG - chr2.4:59294546-59294565 MS.gene003314:CDS 40.0%
ATGAATGTGGATCTGATGGT+GGG - chr2.4:59293888-59293907 MS.gene003314:CDS 40.0%
CACAACACAACTGTTTGATG+AGG + chr2.4:59294494-59294513 None:intergenic 40.0%
CACCCTCACTACAAAAATCA+GGG + chr2.4:59293802-59293821 None:intergenic 40.0%
CCACATTCATCCCAATCTTT+AGG + chr2.4:59293878-59293897 None:intergenic 40.0%
CCTAAAGATTGGGATGAATG+TGG - chr2.4:59293875-59293894 MS.gene003314:CDS 40.0%
CTCATCAAACAGTTGTGTTG+TGG - chr2.4:59294492-59294511 MS.gene003314:CDS 40.0%
GAAGAGAAATAAGAAGCCCA+AGG - chr2.4:59294450-59294469 MS.gene003314:CDS 40.0%
GAGTCGAGACTTAAAGCATA+TGG - chr2.4:59294595-59294614 MS.gene003314:CDS 40.0%
GAGTGAAGATGAGGAATTTG+AGG - chr2.4:59293256-59293275 MS.gene003314:CDS 40.0%
GATGCAAGAGATACAAGAGA+AGG - chr2.4:59293625-59293644 MS.gene003314:CDS 40.0%
GGAAGGAGAAACTGAAAAAG+AGG - chr2.4:59293976-59293995 MS.gene003314:CDS 40.0%
GGCAATACGACAAAAGGAAA+GGG - chr2.4:59293571-59293590 MS.gene003314:CDS 40.0%
GTAGGTTTGAGCATAACAAG+AGG - chr2.4:59292730-59292749 MS.gene003314:CDS 40.0%
TACTTGGGCTTCCTAAAGAT+TGG - chr2.4:59293864-59293883 MS.gene003314:CDS 40.0%
TCACCCTCACTACAAAAATC+AGG + chr2.4:59293803-59293822 None:intergenic 40.0%
TGAGGAATTTGAGGAGAAAC+TGG - chr2.4:59293265-59293284 MS.gene003314:CDS 40.0%
TTCACCTGTCTTCTCCTTTA+AGG + chr2.4:59293225-59293244 None:intergenic 40.0%
! AGTGATGATGATCTCATGGA+AGG - chr2.4:59293959-59293978 MS.gene003314:CDS 40.0%
! GAAGCGTAAAACTGCACTTT+TGG - chr2.4:59294030-59294049 MS.gene003314:CDS 40.0%
! GACCCTGATTTTTGTAGTGA+GGG - chr2.4:59293797-59293816 MS.gene003314:CDS 40.0%
! GATTTTGAATGTTGGAGAGG+AGG - chr2.4:59293373-59293392 MS.gene003314:CDS 40.0%
! GCAAAGCATTTGATTGAGGA+GGG - chr2.4:59293050-59293069 MS.gene003314:CDS 40.0%
! GGAGATTTTGAATGTTGGAG+AGG - chr2.4:59293370-59293389 MS.gene003314:CDS 40.0%
! TATGCTTTAAGTCTCGACTC+TGG + chr2.4:59294595-59294614 None:intergenic 40.0%
! TGACCCTGATTTTTGTAGTG+AGG - chr2.4:59293796-59293815 MS.gene003314:CDS 40.0%
!! ATAGTATCTCTGGAGTATCC+AGG + chr2.4:59294171-59294190 None:intergenic 40.0%
!! TATTGGTTCACCTTCACACT+CGG - chr2.4:59292797-59292816 MS.gene003314:CDS 40.0%
AAAGAGCAAAGAGGAGAGAG+AGG - chr2.4:59293550-59293569 MS.gene003314:CDS 45.0%
AAGCCAAGAGAAGGGAAAAC+AGG - chr2.4:59294554-59294573 MS.gene003314:CDS 45.0%
ACGACAAAAGGAAAGGGATG+AGG - chr2.4:59293577-59293596 MS.gene003314:CDS 45.0%
ACTTGCTCCTTACCAAGAAG+AGG - chr2.4:59294234-59294253 MS.gene003314:CDS 45.0%
AGAAAAGGAGTTTGACCCAG+AGG - chr2.4:59293715-59293734 MS.gene003314:CDS 45.0%
AGAGCAGAGAAAGAGCAAAG+AGG - chr2.4:59293541-59293560 MS.gene003314:CDS 45.0%
AGCCAAGAGAAGGGAAAACA+GGG - chr2.4:59294555-59294574 MS.gene003314:CDS 45.0%
ATCCTTCGTGCAGCAAGTTT+GGG - chr2.4:59294310-59294329 MS.gene003314:CDS 45.0%
ATTATGAGGACACAGTTGGG+GGG - chr2.4:59294095-59294114 MS.gene003314:CDS 45.0%
CAAGGTAGAGAGTGAAGATG+AGG - chr2.4:59293247-59293266 MS.gene003314:CDS 45.0%
CTCACCTTAAAGGAGAAGAC+AGG - chr2.4:59293218-59293237 MS.gene003314:CDS 45.0%
CTGAAAGCAGCAGAAAAGGA+TGG - chr2.4:59293176-59293195 MS.gene003314:CDS 45.0%
GACTTATGCTGATGAGCAAG+AGG - chr2.4:59293136-59293155 MS.gene003314:CDS 45.0%
GAGAGAGGCAATACGACAAA+AGG - chr2.4:59293565-59293584 MS.gene003314:CDS 45.0%
GATGAATGTGGATCTGATGG+TGG - chr2.4:59293887-59293906 MS.gene003314:CDS 45.0%
GATTATGAGGACACAGTTGG+GGG - chr2.4:59294094-59294113 MS.gene003314:CDS 45.0%
GGTTTGAGCATAACAAGAGG+CGG - chr2.4:59292733-59292752 MS.gene003314:CDS 45.0%
GTATCCAGGCCAAATCTGTT+AGG + chr2.4:59294157-59294176 None:intergenic 45.0%
GTTTGAGCATAACAAGAGGC+GGG - chr2.4:59292734-59292753 MS.gene003314:CDS 45.0%
TAGACGAGGAATATGCTCGT+AGG - chr2.4:59292712-59292731 MS.gene003314:CDS 45.0%
TAGTGAGGGTGAAGAAGACA+TGG - chr2.4:59293811-59293830 MS.gene003314:CDS 45.0%
TCATCATCTTCCGAGTGTGA+AGG + chr2.4:59292810-59292829 None:intergenic 45.0%
TGAAAAAGAGGACATTCCCG+AGG - chr2.4:59293988-59294007 MS.gene003314:CDS 45.0%
TGAGATGGAAATTGAGAGGC+AGG - chr2.4:59293418-59293437 MS.gene003314:CDS 45.0%
TGAGCAAGAGGAGTTGAAAC+AGG - chr2.4:59293148-59293167 MS.gene003314:CDS 45.0%
TGGGATGAATGTGGATCTGA+TGG - chr2.4:59293884-59293903 MS.gene003314:CDS 45.0%
! ATTGAGGAGGGACCAGATTT+TGG - chr2.4:59293062-59293081 MS.gene003314:CDS 45.0%
! GGCAAAGCATTTGATTGAGG+AGG - chr2.4:59293049-59293068 MS.gene003314:CDS 45.0%
! GGTGGCAAAGCATTTGATTG+AGG - chr2.4:59293046-59293065 MS.gene003314:CDS 45.0%
!! GACTTGCTAGAATCAGCCTT+GGG + chr2.4:59294469-59294488 None:intergenic 45.0%
!! TGACTTGCTAGAATCAGCCT+TGG + chr2.4:59294470-59294489 None:intergenic 45.0%
!!! TGGATCTGATGGTGGGTTTT+TGG - chr2.4:59293895-59293914 MS.gene003314:CDS 45.0%
AAAGAGGACATTCCCGAGGA+AGG - chr2.4:59293992-59294011 MS.gene003314:CDS 50.0%
AAATCCAGGGGATAGAGTGC+TGG - chr2.4:59293466-59293485 MS.gene003314:CDS 50.0%
AGCAGCAGAAAAGGATGGCT+TGG - chr2.4:59293181-59293200 MS.gene003314:CDS 50.0%
AGGAAGGTAGTAGTCGGAAG+AGG - chr2.4:59294008-59294027 MS.gene003314:CDS 50.0%
CATGCTGAAGATGAGGGCTA+AGG - chr2.4:59294285-59294304 MS.gene003314:CDS 50.0%
CTTCCGACTACTACCTTCCT+CGG + chr2.4:59294008-59294027 None:intergenic 50.0%
GATCCTTCGTGCAGCAAGTT+TGG - chr2.4:59294309-59294328 MS.gene003314:CDS 50.0%
GCAGCAGAAAAGGATGGCTT+GGG - chr2.4:59293182-59293201 MS.gene003314:CDS 50.0%
GGAGAAGACAGGTGAAGACA+AGG - chr2.4:59293229-59293248 MS.gene003314:CDS 50.0%
TTATGAGGACACAGTTGGGG+GGG - chr2.4:59294096-59294115 MS.gene003314:CDS 50.0%
TTCCCGAGGAAGGTAGTAGT+CGG - chr2.4:59294002-59294021 MS.gene003314:CDS 50.0%
TTCCGACTACTACCTTCCTC+GGG + chr2.4:59294007-59294026 None:intergenic 50.0%
! AAAGGATGGCTTGGGAGATG+AGG - chr2.4:59293190-59293209 MS.gene003314:CDS 50.0%
! AATCCAGGGGATAGAGTGCT+GGG - chr2.4:59293467-59293486 MS.gene003314:CDS 50.0%
! AGCCCTGTTTTCCCTTCTCT+TGG + chr2.4:59294560-59294579 None:intergenic 50.0%
!! TACCCAAACTTGCTGCACGA+AGG + chr2.4:59294315-59294334 None:intergenic 50.0%
GGTCATGCTCGTAAGGTGGA+GGG - chr2.4:59293488-59293507 MS.gene003314:CDS 55.0%
TGAGCATAACAAGAGGCGGG+AGG - chr2.4:59292737-59292756 MS.gene003314:CDS 55.0%
TGCTCGTAAGGTGGAGGGTT+CGG - chr2.4:59293493-59293512 MS.gene003314:CDS 55.0%
! AGTGCTGGGTCATGCTCGTA+AGG - chr2.4:59293481-59293500 MS.gene003314:CDS 55.0%
GCTGGGTCATGCTCGTAAGG+TGG - chr2.4:59293484-59293503 MS.gene003314:CDS 60.0%
GGGTCATGCTCGTAAGGTGG+AGG - chr2.4:59293487-59293506 MS.gene003314:CDS 60.0%
TGACCCAGCACTCTATCCCC+TGG + chr2.4:59293473-59293492 None:intergenic 60.0%
!! GTAAGGTGGAGGGTTCGGTG+AGG - chr2.4:59293498-59293517 MS.gene003314:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 59292651 59294630 59292651 ID=MS.gene003314
chr2.4 mRNA 59292651 59294630 59292651 ID=MS.gene003314.t1;Parent=MS.gene003314
chr2.4 exon 59292651 59294630 59292651 ID=MS.gene003314.t1.exon1;Parent=MS.gene003314.t1
chr2.4 CDS 59292651 59294630 59292651 ID=cds.MS.gene003314.t1;Parent=MS.gene003314.t1
Gene Sequence

>MS.gene003314

ATGACTCAGAACATTTTTGATGGCAGTGATTCAGAAAATGAAGATATCTCAAATATCAAGATAGACGAGGAATATGCTCGTAGGTTTGAGCATAACAAGAGGCGGGAGGATCTCCAACGTTATGAAGAATTGAAAAAGAAAGGGGTTATTGGTTCACCTTCACACTCGGAAGATGATGAATCTGAGTCTGAATCTTCTGATGATGACGATGTGAACAATTTCAACTCTAAGAGTGATAAGGAATTCTTTGATGCTTTGATTAAGGTGAAGAAGAAAGATCCTGTTCTTAAGCAAAAGGATGTTAAGCTTTTTGAATCAGACCATAGTAGTGAAGATGAAAGTGATGATGAGAAGAGTAAAGATAAGGAGAAAAAGTCAATGTTTTTGAAGGATGTGGTGGCAAAGCATTTGATTGAGGAGGGACCAGATTTTGGTGATAAAGAGGATGAAACAAATGAGAAAGGTATATCAAATGGTGGTAAGAAGACTTATGCTGATGAGCAAGAGGAGTTGAAACAGGCTTTTCTGAAAGCAGCAGAAAAGGATGGCTTGGGAGATGAGGATTTTCTCACCTTAAAGGAGAAGACAGGTGAAGACAAGGTAGAGAGTGAAGATGAGGAATTTGAGGAGAAACTGGATGCATATTTTGATGATTCAAACGAAAATTCTTTATTTCTAAAAAACTATTTCAAGAACAAAATGTGGGTAGACAAGAATGCGGAGATTTTGAATGTTGGAGAGGAGGATTTGCAAGAGATTTCAGAAGATGAGATGGAAATTGAGAGGCAGGAAGAGTATGAAGTTAGTTTTCAGGAAAATCCAGGGGATAGAGTGCTGGGTCATGCTCGTAAGGTGGAGGGTTCGGTGAGGAAAAAGACAAACTCAAGGAAAGAGCAGAGAAAGAGCAAAGAGGAGAGAGAGGCAATACGACAAAAGGAAAGGGATGAGGAGTTGAAACATTTAAAGAATGTGAAGATGCAAGAGATACAAGAGAAGGTTAAAAAGATAAAGAAGATTGCAGGGATCAACGATGATGATATTATCCCTTTGTCTACTGCAGAATTAGAAAAGGAGTTTGACCCAGAGGAATATGACAGAATGATGAAGAAAGCATTTGATGCGAAGTATTATAACGAAGAGGATGTTGACCCTGATTTTTGTAGTGAGGGTGAAGAAGACATGGAGAAGCCAGATTTTGAAAAAGAGGATGATTTACTTGGGCTTCCTAAAGATTGGGATGAATGTGGATCTGATGGTGGGTTTTTGGCTGCAAGAGAAAAAGCATTGAAAGCAAAGATTGAAAATACTAGTGATGATGATCTCATGGAAGGAGAAACTGAAAAAGAGGACATTCCCGAGGAAGGTAGTAGTCGGAAGAGGAAGCGTAAAACTGCACTTTTGGAGAAAGCAAGACAAATGATGATGGATGAGTTTTATAAATTAGATTATGAGGACACAGTTGGGGGGGATCTGAAGACAAGATTCAAGTATACCAAAACAAAACCTAACAGATTTGGCCTGGATACTCCAGAGATACTATTGATCGATGATAAGGAACTGAATCAACATATTTCCTTGAAAAAACTTGCTCCTTACCAAGAAGAGGAATTGAAGCTTAGCAAAAAAAAAAGATACATGCTGAAGATGAGGGCTAAGGAGATCCTTCGTGCAGCAAGTTTGGGTAAGAAGAAAAAGAGGTTAAAGGTTGATTCTAGCAAATCAACTCAAGTTGATTCTAGCAAGTTAACTTCAAACAATGTTGTGGAGGAAGAAAAAGCAAACACAGCAAGTTTGGATAAGAAGAGAAATAAGAAGCCCAAGGCTGATTCTAGCAAGTCAACCTCATCAAACAGTTGTGTTGTGGAGGATGAAAAATCTAACACAGAAGAATCAAAATCAAGGAAAGCCAAGAGAAGGGAAAACAGGGCTAAATTAAAGTTGCCAGAGTCGAGACTTAAAGCATATGGAAAGACAACCTGA
Protein sequence

>MS.gene003314.t1

MTQNIFDGSDSENEDISNIKIDEEYARRFEHNKRREDLQRYEELKKKGVIGSPSHSEDDESESESSDDDDVNNFNSKSDKEFFDALIKVKKKDPVLKQKDVKLFESDHSSEDESDDEKSKDKEKKSMFLKDVVAKHLIEEGPDFGDKEDETNEKGISNGGKKTYADEQEELKQAFLKAAEKDGLGDEDFLTLKEKTGEDKVESEDEEFEEKLDAYFDDSNENSLFLKNYFKNKMWVDKNAEILNVGEEDLQEISEDEMEIERQEEYEVSFQENPGDRVLGHARKVEGSVRKKTNSRKEQRKSKEEREAIRQKERDEELKHLKNVKMQEIQEKVKKIKKIAGINDDDIIPLSTAELEKEFDPEEYDRMMKKAFDAKYYNEEDVDPDFCSEGEEDMEKPDFEKEDDLLGLPKDWDECGSDGGFLAAREKALKAKIENTSDDDLMEGETEKEDIPEEGSSRKRKRKTALLEKARQMMMDEFYKLDYEDTVGGDLKTRFKYTKTKPNRFGLDTPEILLIDDKELNQHISLKKLAPYQEEELKLSKKKRYMLKMRAKEILRAASLGKKKKRLKVDSSKSTQVDSSKLTSNNVVEEEKANTASLDKKRNKKPKADSSKSTSSNSCVVEDEKSNTEESKSRKAKRRENRAKLKLPESRLKAYGKTT