Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95471.t1 | XP_003590720.2 | 94.6 | 1294 | 69 | 1 | 1 | 1293 | 1 | 1294 | 0.00E+00 | 2288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95471.t1 | Q5ZKD5 | 25.4 | 1298 | 786 | 35 | 5 | 1245 | 91 | 1263 | 9.7e-77 | 290.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95471.t1 | G7I2N3 | 94.6 | 1294 | 69 | 1 | 1 | 1293 | 1 | 1294 | 0.0e+00 | 2288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene95471 | 0.842533 | 2.44E-58 | -1.69E-46 |
| MS.gene049825 | MS.gene95471 | 0.821764 | 3.39E-53 | -1.69E-46 |
| MS.gene055808 | MS.gene95471 | 0.810427 | 1.16E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene95471 | 0.800493 | 1.40E-48 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95471.t1 | MTR_1g072970 | 94.513 | 1294 | 70 | 1 | 1 | 1293 | 1 | 1294 | 0.0 | 2495 |
| MS.gene95471.t1 | MTR_1g072890 | 86.241 | 1301 | 155 | 5 | 1 | 1290 | 1 | 1288 | 0.0 | 2259 |
| MS.gene95471.t1 | MTR_1g072890 | 84.913 | 1034 | 132 | 5 | 1 | 1023 | 1 | 1021 | 0.0 | 1753 |
| MS.gene95471.t1 | MTR_1g053255 | 28.624 | 1083 | 715 | 24 | 7 | 1066 | 3 | 1050 | 6.35e-104 | 357 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95471.t1 | AT2G34357 | 51.244 | 1286 | 575 | 21 | 16 | 1288 | 24 | 1270 | 0.0 | 1175 |
| MS.gene95471.t1 | AT4G23540 | 28.741 | 1096 | 698 | 26 | 5 | 1061 | 10 | 1061 | 3.62e-111 | 377 |
Find 256 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCAGCTTCCAAATTTAA+TGG | 0.155327 | 1.3:+52541133 | None:intergenic |
| ATCCCTTATACCATTAAATT+TGG | 0.179078 | 1.3:-52541143 | MS.gene95471:CDS |
| CTTCCAAATTTAATGGTATA+AGG | 0.189743 | 1.3:+52541140 | None:intergenic |
| ACCTCATCAACTGAGATTTC+TGG | 0.202537 | 1.3:-52543548 | MS.gene95471:CDS |
| CTGCTGTTCTTCTTCGCTTC+TGG | 0.206041 | 1.3:+52533793 | None:intergenic |
| TGAAGTTCTGCTTGGGCTTA+TGG | 0.209306 | 1.3:-52539044 | MS.gene95471:CDS |
| CAGATCTTTCAACAAATTAT+TGG | 0.220117 | 1.3:+52540587 | None:intergenic |
| ATTTATCCGTCTCAAATAAA+TGG | 0.226752 | 1.3:-52541111 | MS.gene95471:CDS |
| CATATTATTCCACGAATATT+AGG | 0.238036 | 1.3:+52540762 | None:intergenic |
| TTTCACATTTGTTGATTCAT+AGG | 0.241660 | 1.3:-52544944 | MS.gene95471:CDS |
| GGCCAATCTTGGTTTATTAA+AGG | 0.244569 | 1.3:-52537374 | MS.gene95471:CDS |
| TTCTCCCTGCAGGCCAATCT+TGG | 0.277069 | 1.3:-52537385 | MS.gene95471:intron |
| ATTATACAAGACTTGTTCTT+TGG | 0.291284 | 1.3:+52540659 | None:intergenic |
| TCCTTTATGTTTCACAAATT+AGG | 0.296330 | 1.3:-52543092 | MS.gene95471:intron |
| CAATCCTTCTGCTTGTGATT+TGG | 0.298079 | 1.3:+52537342 | None:intergenic |
| TGCCTCTGTGGCTGCAAGTA+TGG | 0.299117 | 1.3:+52543295 | None:intergenic |
| TTGGAGAGTGGTGTTGTTCA+TGG | 0.303206 | 1.3:-52544978 | MS.gene95471:CDS |
| AGTAATAAAGCCAGCATTGC+AGG | 0.304957 | 1.3:-52539402 | MS.gene95471:intron |
| TCGCTTCTGGCCTTTAGTTC+CGG | 0.307234 | 1.3:+52533806 | None:intergenic |
| ACACACTTGTTTACTCTTTG+TGG | 0.311562 | 1.3:-52540878 | MS.gene95471:CDS |
| TGATGAAAGTTTGATTAAAC+AGG | 0.311799 | 1.3:-52543246 | MS.gene95471:CDS |
| ATTATCACTATGCTGCTTCT+TGG | 0.316544 | 1.3:-52543136 | MS.gene95471:CDS |
| TGTTATGTTGCCATCGAGTT+TGG | 0.322470 | 1.3:-52544997 | MS.gene95471:CDS |
| AAATCAGCAGTGTCTTCATT+TGG | 0.329028 | 1.3:-52534356 | MS.gene95471:CDS |
| AGCTTGAACCTTATGCATAT+TGG | 0.332365 | 1.3:-52533689 | MS.gene95471:CDS |
| TCCCTTGCTCGTTCAATCAT+AGG | 0.348873 | 1.3:+52541022 | None:intergenic |
| AACCTTTAATAAACCAAGAT+TGG | 0.349531 | 1.3:+52537372 | None:intergenic |
| GTTCCTGTGCGATGCGGTCC+AGG | 0.354576 | 1.3:+52545141 | None:intergenic |
| TAGACAAAACAAGAACATTC+AGG | 0.356460 | 1.3:-52540718 | MS.gene95471:CDS |
| AATAAAGGCAATCAAGTCTA+TGG | 0.356481 | 1.3:+52538987 | None:intergenic |
| CTGTGGTAAATATGACAAAA+AGG | 0.357847 | 1.3:-52533599 | MS.gene95471:CDS |
| ACCTAATTTGTGAAACATAA+AGG | 0.367288 | 1.3:+52543091 | None:intergenic |
| TTCTGAATCGTCTACTTTGT+TGG | 0.367478 | 1.3:+52539699 | None:intergenic |
| TCCATCCACATTTGTCCTGC+TGG | 0.368547 | 1.3:-52537517 | MS.gene95471:CDS |
| GAACAGGGCTTATGAAATTC+TGG | 0.371930 | 1.3:-52537879 | MS.gene95471:CDS |
| CCGTTATGCGCCTTGTGACT+AGG | 0.376054 | 1.3:+52543660 | None:intergenic |
| CATGGACTATCAAGAAATAA+AGG | 0.376348 | 1.3:+52538972 | None:intergenic |
| CGAAGCTTGAAGTTCTGCTT+GGG | 0.378220 | 1.3:-52539051 | MS.gene95471:CDS |
| GTGTTGGTGGTTTGCGGCGG+TGG | 0.378305 | 1.3:+52545256 | None:intergenic |
| TTCCAAATTTAATGGTATAA+GGG | 0.379913 | 1.3:+52541141 | None:intergenic |
| CAAGAATATACAAAACTTGT+TGG | 0.382877 | 1.3:+52543774 | None:intergenic |
| TTCTCCTTCAACACAGCAAC+GGG | 0.387031 | 1.3:+52545053 | None:intergenic |
| ATTCTAAAACAGTATATTGT+TGG | 0.389703 | 1.3:-52541079 | MS.gene95471:CDS |
| AACACCTCAAGTAGTGCTTC+GGG | 0.393913 | 1.3:+52543593 | None:intergenic |
| GGGTCAGCTCTTGTTGCAAT+GGG | 0.394671 | 1.3:-52541181 | MS.gene95471:CDS |
| CTTGCAGACAAATGACTATC+AGG | 0.396859 | 1.3:+52533831 | None:intergenic |
| AATCAGCAGTGTCTTCATTT+GGG | 0.400201 | 1.3:-52534355 | MS.gene95471:intron |
| ATCATTTGCATTTGCTCCAC+CGG | 0.400256 | 1.3:+52543850 | None:intergenic |
| GAGTCGTCGGCCACAGCAAC+GGG | 0.401771 | 1.3:-52533646 | MS.gene95471:CDS |
| CATTCAGGATTCAAATGATA+AGG | 0.406494 | 1.3:-52540703 | MS.gene95471:CDS |
| TTTCCTCACTGAGATAATAC+TGG | 0.407293 | 1.3:-52538764 | MS.gene95471:CDS |
| TTATCACTATGCTGCTTCTT+GGG | 0.410599 | 1.3:-52543135 | MS.gene95471:CDS |
| CGTTATGCGCCTTGTGACTA+GGG | 0.411687 | 1.3:+52543661 | None:intergenic |
| AATTTCATAAGCCCTGTTCC+TGG | 0.412637 | 1.3:+52537883 | None:intergenic |
| TGCAGCTTCATGCGTGCCTT+GGG | 0.415736 | 1.3:-52541201 | MS.gene95471:intron |
| TTCGCAGCTTCATTGTTGCC+TGG | 0.416944 | 1.3:-52539458 | MS.gene95471:CDS |
| AGTTCCCGTTGCTGTGTTGA+AGG | 0.418162 | 1.3:-52545057 | MS.gene95471:CDS |
| AGATGATGGTGGTTGTTTGC+AGG | 0.419096 | 1.3:-52540538 | MS.gene95471:intron |
| TTTGAAGTGGTTAAGAATGT+TGG | 0.419659 | 1.3:+52543706 | None:intergenic |
| ATCTCAGTGAGGAAAACTTC+TGG | 0.421709 | 1.3:+52538772 | None:intergenic |
| AAGGTTGCTCGCGGCATTGT+TGG | 0.422695 | 1.3:-52537633 | MS.gene95471:CDS |
| AGGTTGCTCGCGGCATTGTT+GGG | 0.423518 | 1.3:-52537632 | MS.gene95471:CDS |
| TGGGTCAGCTCTTGTTGCAA+TGG | 0.423842 | 1.3:-52541182 | MS.gene95471:CDS |
| ACTGATTGAGACACGTCATT+TGG | 0.425094 | 1.3:+52539655 | None:intergenic |
| ATAGTGACCTGTTTCCTAAA+GGG | 0.427478 | 1.3:+52541820 | None:intergenic |
| CTTGTATAATTACTCGCAAC+AGG | 0.428147 | 1.3:-52540646 | MS.gene95471:CDS |
| TAACACCTCAAGTAGTGCTT+CGG | 0.429890 | 1.3:+52543592 | None:intergenic |
| AGACACAGCTAAAAGTTTCA+AGG | 0.430200 | 1.3:-52540823 | MS.gene95471:CDS |
| AGAAGTTTCATATGCTCTTC+AGG | 0.432031 | 1.3:+52537047 | None:intergenic |
| CTTAGACAAACCTGCAATGC+TGG | 0.433016 | 1.3:+52539392 | None:intergenic |
| CTTGAAGGCAAGAGTGTCTC+TGG | 0.435014 | 1.3:-52533576 | MS.gene95471:CDS |
| CAGAGCCTGGTAGGGCTGGT+AGG | 0.435386 | 1.3:-52533773 | MS.gene95471:CDS |
| GTGCAGCTTCATGCGTGCCT+TGG | 0.435418 | 1.3:-52541202 | MS.gene95471:intron |
| TCGGCCACAGCAACGGGCTG+CGG | 0.436473 | 1.3:-52533640 | MS.gene95471:CDS |
| TCGAAGCTTGAAGTTCTGCT+TGG | 0.439302 | 1.3:-52539052 | MS.gene95471:CDS |
| AGAGCCTGGTAGGGCTGGTA+GGG | 0.440903 | 1.3:-52533772 | MS.gene95471:CDS |
| TGCTTGACCGGCAGAAAGTA+AGG | 0.447864 | 1.3:-52534004 | MS.gene95471:CDS |
| GGATTATAGTAGGCAAGATA+TGG | 0.448758 | 1.3:-52540682 | MS.gene95471:CDS |
| ATGAGGTGGATAGGTGGATT+AGG | 0.450128 | 1.3:+52545119 | None:intergenic |
| ACTGCTCGCTTGCTTGATGC+TGG | 0.451373 | 1.3:-52543509 | MS.gene95471:CDS |
| GATTATTATTTGTAGGATGT+TGG | 0.451539 | 1.3:-52539299 | MS.gene95471:intron |
| GAAAGGAAGGCATTCTTTCA+AGG | 0.451620 | 1.3:+52543748 | None:intergenic |
| ATGAGTCGATGGCGATGGCC+TGG | 0.451976 | 1.3:-52538795 | MS.gene95471:CDS |
| TGAATTGCATATATCATCGT+TGG | 0.452485 | 1.3:+52545301 | None:intergenic |
| CTGCGCCACATGCTCATCCC+TGG | 0.456440 | 1.3:-52545159 | MS.gene95471:CDS |
| TTCAGGCAGGACAGCTTTAA+CGG | 0.457383 | 1.3:+52537064 | None:intergenic |
| ACAGAGGTGTTGGTGGTTTG+CGG | 0.458316 | 1.3:+52545250 | None:intergenic |
| GCAATCAAGTCTATGGCGTT+TGG | 0.458967 | 1.3:+52538994 | None:intergenic |
| TGAGATAATACTGGCTCTAA+AGG | 0.459212 | 1.3:-52538755 | MS.gene95471:CDS |
| AAAGAAGAACCTAATATTCG+TGG | 0.460647 | 1.3:-52540771 | MS.gene95471:CDS |
| AAGCTGGTGGAGATATTAAG+AGG | 0.462330 | 1.3:-52533719 | MS.gene95471:CDS |
| AGTCAAAGGTTTAGCTCGCT+TGG | 0.462774 | 1.3:-52537583 | MS.gene95471:CDS |
| TTCACATTTGTTGATTCATA+GGG | 0.466227 | 1.3:-52544943 | MS.gene95471:CDS |
| CAACGGGCTGCGGCAAGAAA+AGG | 0.470152 | 1.3:-52533630 | MS.gene95471:CDS |
| ACAGCAGAGCCTGGTAGGGC+TGG | 0.471151 | 1.3:-52533777 | MS.gene95471:CDS |
| TTCATAGGGATAGTGTTCAT+TGG | 0.476869 | 1.3:-52544929 | MS.gene95471:CDS |
| ACTGTTGCACTTATCATATG+TGG | 0.478961 | 1.3:+52537603 | None:intergenic |
| AGTATACCTCTCATAAAGTA+AGG | 0.479237 | 1.3:+52541886 | None:intergenic |
| AAGAATATACAAAACTTGTT+GGG | 0.480754 | 1.3:+52543775 | None:intergenic |
| CTTCATTTACAGGGTACAAT+CGG | 0.483174 | 1.3:-52539808 | MS.gene95471:intron |
| ACGTGTCTCAATCAGTTCTC+AGG | 0.484837 | 1.3:-52539648 | MS.gene95471:intron |
| GCAAAGTAGACGGAACCATA+TGG | 0.487129 | 1.3:-52534477 | MS.gene95471:CDS |
| ACTTGAAAGGTTTCTACTGC+TGG | 0.487430 | 1.3:-52543873 | MS.gene95471:CDS |
| AGCCACTTGATTTGCTTGAC+CGG | 0.490414 | 1.3:-52534016 | MS.gene95471:CDS |
| TGGTTGAGTGTTTGTTTAAG+TGG | 0.490627 | 1.3:-52537300 | MS.gene95471:CDS |
| GATAGTGACCTGTTTCCTAA+AGG | 0.491830 | 1.3:+52541819 | None:intergenic |
| TTGGATGAATCAAGGATGTC+AGG | 0.493009 | 1.3:-52543206 | MS.gene95471:CDS |
| GTTTCATATGCTCTTCAGGC+AGG | 0.494298 | 1.3:+52537051 | None:intergenic |
| TTCTCTTCCAACAGGTAAGA+AGG | 0.494757 | 1.3:-52543982 | MS.gene95471:intron |
| CGTTTGATCATCCGTGAGGA+AGG | 0.495561 | 1.3:-52533906 | MS.gene95471:CDS |
| TCGTTTGTTCCCCTTCCTCA+CGG | 0.496835 | 1.3:+52533895 | None:intergenic |
| GCAGTAGAAACCTTTCAAGT+AGG | 0.497113 | 1.3:+52543876 | None:intergenic |
| AGTCATTTGTCTGCAAGGTC+CGG | 0.497944 | 1.3:-52533825 | MS.gene95471:CDS |
| AGGTTGCAACAGAAAATTTG+AGG | 0.498991 | 1.3:-52540626 | MS.gene95471:CDS |
| AACAGAAAATTTGAGGGCAC+TGG | 0.500332 | 1.3:-52540619 | MS.gene95471:CDS |
| AGCAAGGGAGAAGGCTCAAA+AGG | 0.502078 | 1.3:-52541008 | MS.gene95471:intron |
| CGAAGAAGAACAGCAGAGCC+TGG | 0.503610 | 1.3:-52533786 | MS.gene95471:CDS |
| AGTGAGGAAAACTTCTGGCC+AGG | 0.505041 | 1.3:+52538777 | None:intergenic |
| GCCTATGATTGAACGAGCAA+GGG | 0.506421 | 1.3:-52541023 | MS.gene95471:CDS |
| CAACACCTCTGTGCCGTCAT+CGG | 0.508265 | 1.3:-52545239 | MS.gene95471:CDS |
| GATCATGGAAGAAGTCGAAA+TGG | 0.508740 | 1.3:-52545348 | None:intergenic |
| GAAGAACAGCAGAGCCTGGT+AGG | 0.508883 | 1.3:-52533782 | MS.gene95471:CDS |
| AAATGATGATGATGGAACTA+AGG | 0.509116 | 1.3:-52543834 | MS.gene95471:CDS |
| AAACGATGGTGATGGAACTA+AGG | 0.509658 | 1.3:-52543801 | MS.gene95471:CDS |
| CCTTTACTTATTAAAATCAA+AGG | 0.510837 | 1.3:-52538918 | MS.gene95471:intron |
| ATGCATATTGGCCACTTGAT+CGG | 0.511215 | 1.3:-52533677 | MS.gene95471:CDS |
| GGGAGTGGATCAGATAACTT+TGG | 0.513350 | 1.3:-52543225 | MS.gene95471:CDS |
| ATCCCTGGACCGCATCGCAC+AGG | 0.513406 | 1.3:-52545144 | MS.gene95471:CDS |
| CAAATGATAAGGATTATAGT+AGG | 0.514770 | 1.3:-52540692 | MS.gene95471:CDS |
| GAAGGATAGTTACAAAATGA+AGG | 0.516245 | 1.3:+52540846 | None:intergenic |
| TGCCTATGATTGAACGAGCA+AGG | 0.516549 | 1.3:-52541024 | MS.gene95471:CDS |
| TTAGATCTGAGAATCTCAAA+AGG | 0.517424 | 1.3:-52533974 | MS.gene95471:CDS |
| TCTCCTCGTCTACAAGTGCA+TGG | 0.518772 | 1.3:+52537846 | None:intergenic |
| AAGTTTGATTAAACAGGGAG+TGG | 0.520498 | 1.3:-52543240 | MS.gene95471:CDS |
| CTGAAATCTACAAAAGATGA+TGG | 0.520706 | 1.3:-52540552 | MS.gene95471:CDS |
| TCTGCAAGGTCCGGAACTAA+AGG | 0.523343 | 1.3:-52533816 | MS.gene95471:CDS |
| AAAGAACTCAAAAGAAAGGC+AGG | 0.523354 | 1.3:-52533524 | MS.gene95471:CDS |
| TCAAGTGGCCAATATGCATA+AGG | 0.523498 | 1.3:+52533681 | None:intergenic |
| CTTGCCGCAGCCCGTTGCTG+TGG | 0.524543 | 1.3:+52533636 | None:intergenic |
| TGGTGGAGATATTAAGAGGA+AGG | 0.524645 | 1.3:-52533715 | MS.gene95471:CDS |
| GAGCAGACCTTACTTTCTGC+CGG | 0.528773 | 1.3:+52533997 | None:intergenic |
| CGTCATCGGTGCAATGTCAC+AGG | 0.529646 | 1.3:-52545225 | MS.gene95471:CDS |
| GATTCGTCAGATAAGTGCTC+AGG | 0.531397 | 1.3:+52534047 | None:intergenic |
| GCAAATGCAAATGATGATGA+TGG | 0.531564 | 1.3:-52543842 | MS.gene95471:CDS |
| GGAATGCTTATCACGAAGTG+TGG | 0.532099 | 1.3:-52537095 | MS.gene95471:CDS |
| CTGTGACATTGCACCGATGA+CGG | 0.533423 | 1.3:+52545226 | None:intergenic |
| ATGATAAGTGCAACAGTCAA+AGG | 0.535437 | 1.3:-52537597 | MS.gene95471:CDS |
| GCTTATCACGAAGTGTGGAT+TGG | 0.537402 | 1.3:-52537090 | MS.gene95471:CDS |
| ATATCTTTCGCATCTAATCC+AGG | 0.541115 | 1.3:+52539440 | None:intergenic |
| CAGGTATTCTGCATCACTAC+CGG | 0.542320 | 1.3:+52534424 | None:intergenic |
| TGTTGGTGTCATGCAGAAGA+AGG | 0.543631 | 1.3:-52539282 | MS.gene95471:CDS |
| TTTGATCATCCGTGAGGAAG+GGG | 0.546352 | 1.3:-52533904 | MS.gene95471:CDS |
| TTCCTGTGCGATGCGGTCCA+GGG | 0.546885 | 1.3:+52545142 | None:intergenic |
| GCATCATAGTCTGAATCAGC+TGG | 0.547628 | 1.3:+52533864 | None:intergenic |
| TATTAGCATCTGCAAGTGAA+GGG | 0.550929 | 1.3:-52543907 | MS.gene95471:CDS |
| TGAGTCGTCGGCCACAGCAA+CGG | 0.551417 | 1.3:-52533647 | MS.gene95471:CDS |
| AGATAACTTTGGATGAATCA+AGG | 0.554149 | 1.3:-52543214 | MS.gene95471:CDS |
| GAATATGCTAGCAGAAAAGC+TGG | 0.555725 | 1.3:-52533735 | MS.gene95471:CDS |
| TTTCTTCAGATTTAGCACCT+TGG | 0.555738 | 1.3:+52534997 | None:intergenic |
| TGACGGCACAGAGGTGTTGG+TGG | 0.556509 | 1.3:+52545243 | None:intergenic |
| GTGCTAAATCTGAAGAAACT+AGG | 0.557613 | 1.3:-52534992 | MS.gene95471:CDS |
| TTTCAAAAGCAACTACATCC+AGG | 0.559469 | 1.3:-52534962 | MS.gene95471:intron |
| CTGATAGTCATTTGTCTGCA+AGG | 0.559535 | 1.3:-52533830 | MS.gene95471:CDS |
| TTGTAGACGAGGAGAGAGGT+GGG | 0.562318 | 1.3:-52537838 | MS.gene95471:CDS |
| TCAACAAACCTTGTGTATGT+TGG | 0.563253 | 1.3:+52537022 | None:intergenic |
| CTATTAGCATCTGCAAGTGA+AGG | 0.563306 | 1.3:-52543908 | MS.gene95471:CDS |
| GGTTGCAACAGAAAATTTGA+GGG | 0.564627 | 1.3:-52540625 | MS.gene95471:CDS |
| TCTTACTGATTCAAGACCGA+AGG | 0.565448 | 1.3:-52544868 | MS.gene95471:intron |
| GGCTGCGGCAAGAAAAGGTA+TGG | 0.566352 | 1.3:-52533625 | MS.gene95471:CDS |
| CGATGACGGCACAGAGGTGT+TGG | 0.566754 | 1.3:+52545240 | None:intergenic |
| GTCAGACGATGATGAGGTGG+AGG | 0.568488 | 1.3:-52533943 | MS.gene95471:CDS |
| GAAAGGTTTCTACTGCTGGC+CGG | 0.570102 | 1.3:-52543869 | MS.gene95471:CDS |
| CTTATTTAAGAAGAAAATGT+CGG | 0.570985 | 1.3:-52539746 | MS.gene95471:CDS |
| TATGGCGTTTGGCAGAAGAA+TGG | 0.572032 | 1.3:+52539005 | None:intergenic |
| TTAAAAGTGCGTCGATGAGG+TGG | 0.574607 | 1.3:+52545105 | None:intergenic |
| AGAATCTCAAAAGGAAGTCA+AGG | 0.575312 | 1.3:-52533965 | MS.gene95471:CDS |
| TTTGGCATCTGAACATGAAG+AGG | 0.575489 | 1.3:-52543318 | MS.gene95471:CDS |
| CTGATTCAGACTATGATGCT+AGG | 0.575801 | 1.3:-52533860 | MS.gene95471:CDS |
| CCTAGTCACAAGGCGCATAA+CGG | 0.576731 | 1.3:-52543660 | MS.gene95471:CDS |
| TCAACTGAGATTTCTGGAGA+TGG | 0.578863 | 1.3:-52543542 | MS.gene95471:CDS |
| GTGGCGCAGAAGTATGCGAC+AGG | 0.579576 | 1.3:+52545173 | None:intergenic |
| CAGCATAGTGATAATCAAGT+AGG | 0.580924 | 1.3:+52543144 | None:intergenic |
| CACCTCAAGTAGTGCTTCGG+GGG | 0.582090 | 1.3:+52543595 | None:intergenic |
| AGATCAAGGAACGGAAAGAG+AGG | 0.582196 | 1.3:-52535022 | MS.gene95471:CDS |
| TAAGCCCTACCAGCCCTACC+AGG | 0.584920 | 1.3:+52533768 | None:intergenic |
| AACTCTCCTTACTTTATGAG+AGG | 0.585087 | 1.3:-52541892 | MS.gene95471:CDS |
| GCAAATGAAAACGATGGTGA+TGG | 0.585140 | 1.3:-52543809 | MS.gene95471:CDS |
| CTTCTCCTTCAACACAGCAA+CGG | 0.586763 | 1.3:+52545052 | None:intergenic |
| GGAGAAGAGAGAGTTTCTAT+CGG | 0.587647 | 1.3:-52545036 | MS.gene95471:CDS |
| GTAGCCAAATCACAAGCAGA+AGG | 0.588275 | 1.3:-52537346 | MS.gene95471:CDS |
| ACAGGATGAGTCGATGGCGA+TGG | 0.588672 | 1.3:-52538800 | MS.gene95471:intron |
| AAGAACAGCAGAGCCTGGTA+GGG | 0.589719 | 1.3:-52533781 | MS.gene95471:CDS |
| AACGATGGTGATGGAACTAA+GGG | 0.589905 | 1.3:-52543800 | MS.gene95471:CDS |
| CATGCTCTTGAGTGCCTCTG+TGG | 0.592318 | 1.3:+52543283 | None:intergenic |
| GTTTGATCATCCGTGAGGAA+GGG | 0.592610 | 1.3:-52533905 | MS.gene95471:CDS |
| TCCAGCAGGACAAATGTGGA+TGG | 0.593882 | 1.3:+52537516 | None:intergenic |
| AGTGCGTCGATGAGGTGGAT+AGG | 0.595819 | 1.3:+52545110 | None:intergenic |
| GATTGAACGAGCAAGGGAGA+AGG | 0.598453 | 1.3:-52541017 | MS.gene95471:CDS |
| TCCAGAAATCTCAGTTGATG+AGG | 0.599100 | 1.3:+52543547 | None:intergenic |
| CTTGTAGACGAGGAGAGAGG+TGG | 0.601164 | 1.3:-52537839 | MS.gene95471:CDS |
| CTCCTCGTCTACAAGTGCAT+GGG | 0.601413 | 1.3:+52537847 | None:intergenic |
| AAGGATTGCAAATGCATCTG+AGG | 0.602595 | 1.3:-52537327 | MS.gene95471:CDS |
| GTCACAAGGCGCATAACGGA+TGG | 0.603827 | 1.3:-52543656 | MS.gene95471:CDS |
| GCGGTCCAGGGATGAGCATG+TGG | 0.606307 | 1.3:+52545154 | None:intergenic |
| ATCGGAAAATGATGAGTCGT+CGG | 0.608141 | 1.3:-52533659 | MS.gene95471:CDS |
| TTGTTAAAAGTGCGTCGATG+AGG | 0.611791 | 1.3:+52545102 | None:intergenic |
| GATGAAAGTTTGATTAAACA+GGG | 0.613341 | 1.3:-52543245 | MS.gene95471:CDS |
| CACTGCTGATTTGAGATGCG+TGG | 0.613580 | 1.3:+52534367 | None:intergenic |
| TGGTAGGGCTTACACTGGGA+AGG | 0.613888 | 1.3:-52533757 | MS.gene95471:CDS |
| GAGGTGTTGGTGGTTTGCGG+CGG | 0.616455 | 1.3:+52545253 | None:intergenic |
| CTGCCGACAGGATGAGTCGA+TGG | 0.616653 | 1.3:-52538806 | MS.gene95471:intron |
| AGGGCTGGTAGGGCTTACAC+TGG | 0.618442 | 1.3:-52533762 | MS.gene95471:CDS |
| ATGCGACAGGTGAAGAAGAG+AGG | 0.618702 | 1.3:+52545186 | None:intergenic |
| TGGGTCAACCCCTAGTCACA+AGG | 0.620843 | 1.3:-52543670 | MS.gene95471:CDS |
| GTCAAGGTCAGACGATGATG+AGG | 0.621447 | 1.3:-52533949 | MS.gene95471:CDS |
| GTTATGCGCCTTGTGACTAG+GGG | 0.624081 | 1.3:+52543662 | None:intergenic |
| CATGGAAGAAGTCGAAATGG+AGG | 0.624158 | 1.3:-52545345 | MS.gene95471:CDS |
| GCGTCGATGAGGTGGATAGG+TGG | 0.624227 | 1.3:+52545113 | None:intergenic |
| AGTGTCTTTAATGATCTCAA+AGG | 0.624487 | 1.3:-52543434 | MS.gene95471:intron |
| AGGTTTCTACTGCTGGCCGG+TGG | 0.625023 | 1.3:-52543866 | MS.gene95471:CDS |
| TTGTGAGCTAAGGTTGCTCG+CGG | 0.627827 | 1.3:-52537642 | MS.gene95471:intron |
| ACACCACTCTCCAAACTCGA+TGG | 0.628441 | 1.3:+52544987 | None:intergenic |
| GAAAGTTTATCAAGACATTG+CGG | 0.639694 | 1.3:+52543942 | None:intergenic |
| GGGCTGGTAGGGCTTACACT+GGG | 0.640050 | 1.3:-52533761 | MS.gene95471:CDS |
| AAGAAAAGGTATGGCAACTG+TGG | 0.640183 | 1.3:-52533616 | MS.gene95471:CDS |
| AAGGTCAGACGATGATGAGG+TGG | 0.640595 | 1.3:-52533946 | MS.gene95471:CDS |
| TCTCTGTCTTAACCCAACAT+CGG | 0.642234 | 1.3:-52543624 | MS.gene95471:CDS |
| TGCCCATGCACTTGTAGACG+AGG | 0.643766 | 1.3:-52537849 | MS.gene95471:CDS |
| AATGATGATGATGGAACTAA+GGG | 0.647447 | 1.3:-52543833 | MS.gene95471:CDS |
| ATTAGCATCTGCAAGTGAAG+GGG | 0.649135 | 1.3:-52543906 | MS.gene95471:CDS |
| GAGGTGGAGGTAGATTCTGA+AGG | 0.649844 | 1.3:-52533930 | MS.gene95471:CDS |
| TGCCGGTCAAGCAAATCAAG+TGG | 0.651293 | 1.3:+52534014 | None:intergenic |
| TTGAGATCATTAAAGACACT+AGG | 0.652067 | 1.3:+52543437 | None:intergenic |
| ATGCCTCTGACTTCAAGACA+TGG | 0.654569 | 1.3:+52538954 | None:intergenic |
| TTGTAAAACAGATCAAGGAA+CGG | 0.654616 | 1.3:-52535031 | MS.gene95471:intron |
| ACACCTCAAGTAGTGCTTCG+GGG | 0.654699 | 1.3:+52543594 | None:intergenic |
| GATAATACTGGCTCTAAAGG+AGG | 0.656500 | 1.3:-52538752 | MS.gene95471:intron |
| TTGCAACAAGAGCTGACCCA+AGG | 0.656828 | 1.3:+52541185 | None:intergenic |
| GAACGGAAAGAGAGGAACCA+AGG | 0.658669 | 1.3:-52535014 | MS.gene95471:CDS |
| ACTTCTGTCCAACATACACA+AGG | 0.667461 | 1.3:-52537030 | MS.gene95471:intron |
| GCACTTGTAGACGAGGAGAG+AGG | 0.679073 | 1.3:-52537842 | MS.gene95471:CDS |
| TGTAGACGAGGAGAGAGGTG+GGG | 0.680978 | 1.3:-52537837 | MS.gene95471:CDS |
| GATTAGGTTCCTGTGCGATG+CGG | 0.681268 | 1.3:+52545135 | None:intergenic |
| TATGCTAGCAGAAAAGCTGG+TGG | 0.683312 | 1.3:-52533732 | MS.gene95471:CDS |
| AGTCCATGTCTTGAAGTCAG+AGG | 0.684722 | 1.3:-52538957 | MS.gene95471:CDS |
| TTGCCATCGAGTTTGGAGAG+TGG | 0.688858 | 1.3:-52544990 | MS.gene95471:CDS |
| GGCCATACTTGCAGCCACAG+AGG | 0.690135 | 1.3:-52543297 | MS.gene95471:CDS |
| AAAGAACAAAGAGATTACCA+AGG | 0.692817 | 1.3:-52537490 | MS.gene95471:intron |
| AGTGATGCAGAATACCTGAA+CGG | 0.696328 | 1.3:-52534419 | MS.gene95471:CDS |
| AAATCTACAAAAGATGATGG+TGG | 0.698268 | 1.3:-52540549 | MS.gene95471:CDS |
| AAGGGAGCAAATGAAAACGA+TGG | 0.708803 | 1.3:-52543815 | MS.gene95471:CDS |
| GAGCCAGTATTATCTCAGTG+AGG | 0.710886 | 1.3:+52538761 | None:intergenic |
| GCAAATGCATCTGAGGAGCG+TGG | 0.728048 | 1.3:-52537320 | MS.gene95471:CDS |
| CGTTTGGCAGAAGAATGGCA+AGG | 0.732352 | 1.3:+52539010 | None:intergenic |
| TTGCACCGATGACGGCACAG+AGG | 0.748299 | 1.3:+52545234 | None:intergenic |
| AGGACGTTTGATCATCCGTG+AGG | 0.749026 | 1.3:-52533910 | MS.gene95471:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 52533506 | 52545366 | 52533506 | ID=MS.gene95471 |
| chr1.3 | mRNA | 52533506 | 52545366 | 52533506 | ID=MS.gene95471.t1;Parent=MS.gene95471 |
| chr1.3 | exon | 52544869 | 52545366 | 52544869 | ID=MS.gene95471.t1.exon1;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52544869 | 52545366 | 52544869 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52543435 | 52543990 | 52543435 | ID=MS.gene95471.t1.exon2;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52543435 | 52543990 | 52543435 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52543093 | 52543344 | 52543093 | ID=MS.gene95471.t1.exon3;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52543093 | 52543344 | 52543093 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52541828 | 52541919 | 52541828 | ID=MS.gene95471.t1.exon4;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52541828 | 52541919 | 52541828 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52541009 | 52541218 | 52541009 | ID=MS.gene95471.t1.exon5;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52541009 | 52541218 | 52541009 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52540539 | 52540928 | 52540539 | ID=MS.gene95471.t1.exon6;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52540539 | 52540928 | 52540539 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52539649 | 52539818 | 52539649 | ID=MS.gene95471.t1.exon7;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52539649 | 52539818 | 52539649 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52539403 | 52539496 | 52539403 | ID=MS.gene95471.t1.exon8;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52539403 | 52539496 | 52539403 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52539253 | 52539306 | 52539253 | ID=MS.gene95471.t1.exon9;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52539253 | 52539306 | 52539253 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52538919 | 52539095 | 52538919 | ID=MS.gene95471.t1.exon10;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52538919 | 52539095 | 52538919 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52538753 | 52538818 | 52538753 | ID=MS.gene95471.t1.exon11;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52538753 | 52538818 | 52538753 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52537805 | 52537918 | 52537805 | ID=MS.gene95471.t1.exon12;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52537805 | 52537918 | 52537805 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52537491 | 52537652 | 52537491 | ID=MS.gene95471.t1.exon13;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52537491 | 52537652 | 52537491 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52537267 | 52537395 | 52537267 | ID=MS.gene95471.t1.exon14;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52537267 | 52537395 | 52537267 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52537031 | 52537132 | 52537031 | ID=MS.gene95471.t1.exon15;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52537031 | 52537132 | 52537031 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52534963 | 52535042 | 52534963 | ID=MS.gene95471.t1.exon16;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52534963 | 52535042 | 52534963 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52534356 | 52534499 | 52534356 | ID=MS.gene95471.t1.exon17;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52534356 | 52534499 | 52534356 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| chr1.3 | exon | 52533506 | 52534097 | 52533506 | ID=MS.gene95471.t1.exon18;Parent=MS.gene95471.t1 |
| chr1.3 | CDS | 52533506 | 52534097 | 52533506 | ID=cds.MS.gene95471.t1;Parent=MS.gene95471.t1 |
| Gene Sequence |
| Protein sequence |
