AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008306

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008306.t1 MTR_3g065080 96.991 432 11 1 1 432 10 439 0.0 843
MS.gene008306.t1 MTR_5g077510 64.598 435 144 7 1 431 11 439 0.0 538
MS.gene008306.t1 MTR_6g071340 48.210 419 199 7 26 431 2 415 7.98e-126 371
MS.gene008306.t1 MTR_5g048050 47.971 419 200 7 26 431 22 435 6.48e-124 367
MS.gene008306.t1 MTR_7g106340 35.225 423 247 10 25 429 4 417 1.40e-60 203
MS.gene008306.t1 MTR_4g085720 32.767 412 247 10 27 427 12 404 4.53e-59 199
MS.gene008306.t1 MTR_2g087350 34.524 420 243 11 24 429 1 402 1.86e-58 197
MS.gene008306.t1 MTR_1g056880 33.937 442 244 11 25 429 3 433 2.74e-58 197
MS.gene008306.t1 MTR_1g056870 33.258 442 247 11 25 429 3 433 1.46e-57 195
MS.gene008306.t1 MTR_1g056840 32.426 441 226 13 25 429 3 407 3.28e-50 175
MS.gene008306.t1 MTR_1g056910 31.750 400 245 13 24 409 1 386 3.70e-48 170
MS.gene008306.t1 MTR_4g051515 27.273 418 267 14 26 422 3 404 1.59e-39 147
MS.gene008306.t1 MTR_4g063800 26.139 417 267 13 18 419 244 634 3.44e-35 138
MS.gene008306.t1 MTR_4g485520 25.592 422 277 11 4 411 80 478 1.96e-34 134
MS.gene008306.t1 MTR_4g485520 25.592 422 277 11 4 411 248 646 8.52e-34 134
MS.gene008306.t1 MTR_7g059405 28.040 403 238 17 29 412 264 633 1.52e-33 133
MS.gene008306.t1 MTR_5g083030 28.193 415 248 13 27 412 291 684 3.43e-33 132
MS.gene008306.t1 MTR_8g092870 26.014 419 278 12 27 428 8 411 1.83e-32 127
MS.gene008306.t1 MTR_3g095730 24.344 419 283 11 27 428 11 412 2.29e-32 127
MS.gene008306.t1 MTR_5g015210 27.132 387 254 8 29 410 67 430 8.33e-29 117
MS.gene008306.t1 MTR_4g107010 31.395 344 196 14 30 353 281 604 2.59e-28 118
MS.gene008306.t1 MTR_3g096370 67.143 70 23 0 26 95 7 76 1.20e-26 111
MS.gene008306.t1 MTR_2g018010 27.900 319 182 11 27 321 290 584 3.18e-26 112
MS.gene008306.t1 MTR_7g078330 24.830 294 203 6 27 317 272 550 1.08e-25 110
MS.gene008306.t1 MTR_4g028960 28.115 313 167 12 13 312 262 529 2.97e-25 108
MS.gene008306.t1 MTR_1g094215 27.168 346 222 9 29 363 64 390 3.87e-25 107
MS.gene008306.t1 MTR_1g094215 27.168 346 222 9 29 363 247 573 5.00e-25 108
MS.gene008306.t1 MTR_4g091880 24.227 388 241 14 28 413 4 340 2.41e-24 103
MS.gene008306.t1 MTR_1g017770 26.521 411 255 11 19 410 31 413 2.65e-24 104
MS.gene008306.t1 MTR_4g091880 25.441 397 253 13 28 413 4 368 1.02e-23 102
MS.gene008306.t1 MTR_3g085610 27.922 308 170 8 30 328 281 545 2.45e-22 100
MS.gene008306.t1 MTR_3g078160 27.077 325 196 12 30 334 276 579 4.84e-21 96.3
MS.gene008306.t1 MTR_3g078160 27.385 325 195 12 30 334 276 579 5.36e-21 96.3
MS.gene008306.t1 MTR_7g053260 53.623 69 32 0 27 95 76 144 3.47e-20 92.8
MS.gene008306.t1 MTR_7g116600 48.421 95 41 3 30 124 79 165 4.86e-20 92.4
MS.gene008306.t1 MTR_5g034440 44.248 113 47 3 33 134 284 391 6.52e-20 92.8
MS.gene008306.t1 MTR_3g078160 52.857 70 33 0 30 99 276 345 1.78e-19 91.3
MS.gene008306.t1 MTR_1g069845 48.571 70 36 0 27 96 122 191 7.81e-19 89.4
MS.gene008306.t1 MTR_1g069845 48.571 70 36 0 27 96 239 308 1.31e-18 89.0
MS.gene008306.t1 MTR_1g069845 48.571 70 36 0 27 96 243 312 1.35e-18 89.0
MS.gene008306.t1 MTR_7g097020 45.946 74 40 0 27 100 239 312 1.79e-18 88.6
MS.gene008306.t1 MTR_1g079450 26.780 295 181 11 27 307 61 334 3.77e-18 86.7
MS.gene008306.t1 MTR_8g080280 39.560 91 49 1 30 120 283 367 9.69e-18 86.3
MS.gene008306.t1 MTR_2g011140 23.846 390 223 11 32 393 18 361 4.32e-17 82.8
MS.gene008306.t1 MTR_8g077205 23.799 458 282 13 27 426 161 609 1.40e-16 82.4
MS.gene008306.t1 MTR_8g077205 23.799 458 282 13 27 426 273 721 1.74e-16 82.4
MS.gene008306.t1 MTR_3g466220 43.243 74 42 0 27 100 210 283 2.29e-15 79.0
MS.gene008306.t1 MTR_3g466220 43.243 74 42 0 27 100 231 304 2.34e-15 79.0
MS.gene008306.t1 MTR_7g117890 28.682 258 151 8 33 285 259 488 2.76e-15 79.0
MS.gene008306.t1 MTR_5g020570 39.535 86 48 2 25 110 269 350 8.65e-15 77.0
MS.gene008306.t1 MTR_5g020570 39.535 86 48 2 25 110 284 365 9.55e-15 77.0
MS.gene008306.t1 MTR_2g096850 45.070 71 39 0 25 95 585 655 1.23e-14 76.6
MS.gene008306.t1 MTR_2g096850 45.070 71 39 0 25 95 736 806 1.45e-14 76.3
MS.gene008306.t1 MTR_1g093965 48.276 58 30 0 42 99 1 58 5.20e-14 70.9
MS.gene008306.t1 MTR_3g096370 69.767 43 13 0 26 68 7 49 8.65e-14 72.0
MS.gene008306.t1 MTR_1g093995 48.276 58 30 0 42 99 1 58 1.86e-13 69.3
MS.gene008306.t1 MTR_1g090320 40.625 96 48 4 24 111 504 598 6.32e-13 71.6
MS.gene008306.t1 MTR_1g090320 40.625 96 48 4 24 111 506 600 6.44e-13 71.6
MS.gene008306.t1 MTR_3g115670 24.841 314 184 12 13 309 12 290 1.08e-12 70.5
MS.gene008306.t1 MTR_8g103227 41.333 75 43 1 28 101 394 468 1.49e-12 70.5
MS.gene008306.t1 MTR_5g032010 34.247 73 48 0 29 101 732 804 4.53e-12 68.6
MS.gene008306.t1 MTR_1g100820 35.000 80 51 1 17 95 635 714 1.27e-11 67.0
MS.gene008306.t1 MTR_1g100820 35.000 80 51 1 17 95 633 712 1.29e-11 67.0
MS.gene008306.t1 MTR_3g008270 43.939 66 36 1 30 95 731 795 1.30e-11 67.0
MS.gene008306.t1 MTR_8g027140 40.278 72 43 0 33 104 263 334 7.45e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008306.t1 AT5G37490 46.265 415 204 8 26 431 28 432 4.04e-111 334
MS.gene008306.t1 AT1G66160 41.966 417 213 9 26 431 30 428 4.19e-101 308
MS.gene008306.t1 AT1G66160 39.568 417 207 10 26 431 30 412 4.88e-89 276
MS.gene008306.t1 AT3G02840 41.908 346 182 7 100 431 36 376 6.68e-75 239
MS.gene008306.t1 AT2G35930 34.679 421 250 8 20 429 3 409 1.69e-64 213
MS.gene008306.t1 AT3G52450 31.778 450 243 12 25 429 3 433 4.59e-57 194
MS.gene008306.t1 AT1G49780 33.166 398 242 11 27 411 12 398 6.17e-54 185
MS.gene008306.t1 AT3G19380 32.732 388 231 12 27 399 12 384 1.22e-53 184
MS.gene008306.t1 AT3G11840 32.159 454 248 13 25 430 6 447 2.06e-47 169
MS.gene008306.t1 AT2G28830 27.804 428 265 14 7 417 166 566 3.08e-35 137
MS.gene008306.t1 AT5G09800 27.525 396 258 11 28 411 10 388 2.41e-34 132
MS.gene008306.t1 AT1G23030 27.861 402 244 14 25 411 237 607 2.60e-33 132
MS.gene008306.t1 AT5G64660 26.536 407 262 11 27 411 8 399 1.93e-32 127
MS.gene008306.t1 AT1G71020 27.470 415 254 14 17 411 83 470 6.52e-32 127
MS.gene008306.t1 AT3G46510 25.505 396 260 12 29 412 256 628 1.22e-31 127
MS.gene008306.t1 AT1G71020 27.470 415 254 14 17 411 231 618 3.07e-31 126
MS.gene008306.t1 AT3G49810 25.700 393 264 6 29 416 64 433 3.65e-31 124
MS.gene008306.t1 AT5G65920 26.076 395 264 7 29 418 60 431 2.02e-29 119
MS.gene008306.t1 AT5G65920 26.076 395 264 7 29 418 60 431 2.02e-29 119
MS.gene008306.t1 AT3G54850 27.456 397 252 13 29 412 248 621 8.84e-28 116
MS.gene008306.t1 AT3G18710 25.990 404 262 12 27 411 10 395 1.31e-26 111
MS.gene008306.t1 AT5G42340 24.727 275 185 7 29 299 290 546 2.92e-22 100
MS.gene008306.t1 AT3G07360 51.316 76 37 0 24 99 69 144 1.09e-20 94.4
MS.gene008306.t1 AT1G29340 49.412 85 42 1 27 110 303 387 1.10e-19 92.0
MS.gene008306.t1 AT4G21350 26.603 312 196 10 29 332 5 291 1.70e-19 90.1
MS.gene008306.t1 AT1G27910 50.000 70 35 0 30 99 280 349 1.33e-18 89.0
MS.gene008306.t1 AT5G01830 43.902 82 46 0 15 96 260 341 1.44e-18 88.6
MS.gene008306.t1 AT1G10560 27.434 339 213 13 35 355 294 617 2.45e-18 88.2
MS.gene008306.t1 AT3G54790 47.945 73 38 0 27 99 200 272 6.71e-18 86.7
MS.gene008306.t1 AT1G67530 45.000 80 40 1 30 109 273 348 7.92e-18 86.3
MS.gene008306.t1 AT3G54790 47.945 73 38 0 27 99 236 308 8.90e-18 86.3
MS.gene008306.t1 AT3G54790 47.945 73 38 0 27 99 236 308 8.90e-18 86.3
MS.gene008306.t1 AT1G67530 45.000 80 40 1 30 109 273 348 1.01e-17 86.3
MS.gene008306.t1 AT1G67530 45.000 80 40 1 30 109 273 348 1.01e-17 86.3
MS.gene008306.t1 AT1G67530 45.000 80 40 1 30 109 273 348 1.01e-17 86.3
MS.gene008306.t1 AT1G24330 47.143 70 37 0 30 99 276 345 1.30e-17 85.9
MS.gene008306.t1 AT1G24330 47.143 70 37 0 30 99 276 345 1.30e-17 85.9
MS.gene008306.t1 AT1G60190 25.145 346 232 12 33 363 282 615 2.97e-17 84.7
MS.gene008306.t1 AT5G67340 46.053 76 41 0 27 102 238 313 6.00e-17 84.0
MS.gene008306.t1 AT5G67340 46.053 76 41 0 27 102 238 313 6.00e-17 84.0
MS.gene008306.t1 AT4G36550 40.278 72 43 0 28 99 218 289 3.52e-15 78.2
MS.gene008306.t1 AT2G45920 39.744 78 47 0 25 102 321 398 8.48e-15 76.3
MS.gene008306.t1 AT1G01660 33.696 92 58 1 9 100 481 569 9.35e-15 76.6
MS.gene008306.t1 AT1G01660 33.696 92 58 1 9 100 480 568 9.42e-15 76.6
MS.gene008306.t1 AT1G01680 38.571 70 43 0 33 102 237 306 9.93e-15 75.1
MS.gene008306.t1 AT2G23140 40.476 84 46 1 27 110 231 310 1.85e-14 76.3
MS.gene008306.t1 AT2G23140 40.476 84 46 1 27 110 228 307 1.86e-14 76.3
MS.gene008306.t1 AT2G23140 40.476 84 46 1 27 110 228 307 1.86e-14 76.3
MS.gene008306.t1 AT2G23140 43.836 73 41 0 27 99 39 111 2.39e-14 75.5
MS.gene008306.t1 AT2G23140 43.836 73 41 0 27 99 39 111 2.39e-14 75.5
MS.gene008306.t1 AT5G57035 40.909 66 39 0 30 95 709 774 8.15e-14 73.9
MS.gene008306.t1 AT1G56030 42.857 70 40 0 24 93 318 387 3.64e-13 71.2
MS.gene008306.t1 AT5G51270 38.824 85 51 1 15 99 721 804 3.90e-13 72.0
MS.gene008306.t1 AT5G51270 38.824 85 51 1 15 99 721 804 3.90e-13 72.0
MS.gene008306.t1 AT5G65500 40.299 67 40 0 29 95 723 789 6.05e-13 71.2
MS.gene008306.t1 AT2G19410 43.939 66 36 1 30 95 712 776 6.33e-13 71.2
MS.gene008306.t1 AT5G65500 40.299 67 40 0 29 95 715 781 6.35e-13 71.2
MS.gene008306.t1 AT2G19410 43.939 66 36 1 30 95 726 790 6.56e-13 71.2
MS.gene008306.t1 AT2G19410 43.939 66 36 1 30 95 747 811 6.57e-13 71.2
MS.gene008306.t1 AT3G61390 32.941 85 57 0 11 95 260 344 1.86e-12 68.9
MS.gene008306.t1 AT3G61390 32.941 85 57 0 11 95 335 419 3.00e-12 68.6
MS.gene008306.t1 AT3G49060 37.879 66 41 0 30 95 726 791 4.96e-12 68.6
MS.gene008306.t1 AT3G49060 37.879 66 41 0 30 95 736 801 5.26e-12 68.6
MS.gene008306.t1 AT3G49060 37.879 66 41 0 30 95 703 768 5.49e-12 68.2
MS.gene008306.t1 AT1G56040 38.571 70 43 0 24 93 348 417 1.00e-11 67.0
MS.gene008306.t1 AT4G25160 40.909 66 38 1 30 95 767 831 7.07e-11 65.1

Find 81 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATTGTGGATGGAGAAAA+AGG 0.208941 3.1:+54543435 MS.gene008306:CDS
ACACCTCGTATACCTGTTTC+AGG 0.250956 3.1:+54542847 MS.gene008306:CDS
GAAAGTTTCTGATTCGTAAC+TGG 0.281347 3.1:-54542733 None:intergenic
AGGAATAAGAGGGTTATAAT+TGG 0.297670 3.1:+54542982 MS.gene008306:CDS
TGAGGAACATGTTGTTGTTT+TGG 0.298411 3.1:+54543062 MS.gene008306:CDS
ATTCCTGTTGATAGAGTTAC+TGG 0.298592 3.1:-54542655 None:intergenic
TGAAAAGGCACTTGGTGTTT+TGG 0.308014 3.1:+54543464 MS.gene008306:CDS
CGTCTTTGAATTGTTTGGTT+TGG 0.322890 3.1:+54543163 MS.gene008306:CDS
AGTGAAAGATTTGTTGAATT+GGG 0.338006 3.1:+54543390 MS.gene008306:CDS
TGGATTGTTAAAACCTCTTT+CGG 0.347696 3.1:+54543108 MS.gene008306:CDS
TGCGTTGAGAATAGTTCTTA+TGG 0.351415 3.1:+54542808 MS.gene008306:CDS
TAGTGAAAGATTTGTTGAAT+TGG 0.351964 3.1:+54543389 MS.gene008306:CDS
GTCTTCGTCTTTGAATTGTT+TGG 0.354146 3.1:+54543158 MS.gene008306:CDS
ACCGTCGCCGTCGCCGGCGC+CGG 0.369835 3.1:-54542560 None:intergenic
CCGTCGCCGTCGCCGGCGCC+GGG 0.370954 3.1:-54542559 None:intergenic
GTTGTCGGGTTGTAGAAATT+TGG 0.371187 3.1:+54542900 MS.gene008306:CDS
GAGAGAGTGAGAGGAATAAG+AGG 0.381694 3.1:+54542971 MS.gene008306:CDS
GACTCACTGGACAACGGAAA+TGG 0.383153 3.1:-54542615 None:intergenic
AAGATTGTTAAGGAACCAAT+TGG 0.383817 3.1:+54543294 MS.gene008306:CDS
GAAGATGCTTTGTGAGAAGA+AGG 0.393306 3.1:+54543614 MS.gene008306:CDS
TGAAGGTGTTGTTGAGGGTT+TGG 0.395400 3.1:+54543269 MS.gene008306:CDS
TGAGTTTGTTGGGAAGATTA+AGG 0.395413 3.1:+54542939 MS.gene008306:CDS
AAGAGGGTTATAATTGGAAA+TGG 0.402171 3.1:+54542988 MS.gene008306:CDS
TGTTTATGTGATTGCAAGAA+AGG 0.404590 3.1:+54543489 MS.gene008306:CDS
TTTGTAGCACTTGACCCAAT+TGG 0.406484 3.1:-54543309 None:intergenic
GCGTTGAGAATAGTTCTTAT+GGG 0.409523 3.1:+54542809 MS.gene008306:CDS
TGTTGGTTATGTTGCAAGTT+GGG 0.414797 3.1:+54543685 MS.gene008306:CDS
AAAGCAAAACACCAACTTCC+CGG 0.416680 3.1:+54542541 MS.gene008306:CDS
CCCGGCGCCGGCGACGGCGA+CGG 0.439081 3.1:+54542559 MS.gene008306:CDS
CGACGGCGACGGTGAATTAA+CGG 0.439854 3.1:+54542570 MS.gene008306:CDS
TTGGGAAGATTAAGGTTTGG+TGG 0.440953 3.1:+54542947 MS.gene008306:CDS
AAGATTTGTTGAATTGGGTT+TGG 0.447166 3.1:+54543395 MS.gene008306:CDS
TTAACCTCGTACCCTGAAAC+AGG 0.448130 3.1:-54542859 None:intergenic
TAATTCACCGTCGCCGTCGC+CGG 0.448430 3.1:-54542566 None:intergenic
CTGGACAACGGAAATGGGTT+GGG 0.448669 3.1:-54542609 None:intergenic
CGTCGCCGGCGCCGGGAAGT+TGG 0.449959 3.1:-54542552 None:intergenic
GGTTTGTACTAGGTTGTTGT+CGG 0.456977 3.1:+54542885 MS.gene008306:CDS
GGGTTTGGTTAAGATTGTTA+AGG 0.460184 3.1:+54543284 MS.gene008306:CDS
GATCCAGTAACTCTATCAAC+AGG 0.465887 3.1:+54542652 MS.gene008306:CDS
ACTCACTGGACAACGGAAAT+GGG 0.466922 3.1:-54542614 None:intergenic
AAGATTGAAGGTGTTGTTGA+GGG 0.472391 3.1:+54543264 MS.gene008306:CDS
ATAGATTCAGCATCGAGAAA+TGG 0.477438 3.1:+54542686 MS.gene008306:CDS
AAAGTTTCTGATTCGTAACT+GGG 0.480832 3.1:-54542732 None:intergenic
AGGTTAATGAGGTTTGTACT+AGG 0.482538 3.1:+54542875 MS.gene008306:CDS
AAAAGTGTGTTGAGTTTGTT+GGG 0.483403 3.1:+54542929 MS.gene008306:CDS
TTGTTGGGAAGATTAAGGTT+TGG 0.492782 3.1:+54542944 MS.gene008306:CDS
GTTTATGTGATTGCAAGAAA+GGG 0.496421 3.1:+54543490 MS.gene008306:CDS
AGAGAGTGAGAGGAATAAGA+GGG 0.507848 3.1:+54542972 MS.gene008306:CDS
GAAGAGTTATCAAAGATTGA+AGG 0.508398 3.1:+54543252 MS.gene008306:CDS
ATCGAGAAATGGATCGAAGC+TGG 0.513343 3.1:+54542697 MS.gene008306:CDS
TTCATCAAGTCTAGACTCAC+TGG 0.517362 3.1:-54542628 None:intergenic
TTGTTGGTTATGTTGCAAGT+TGG 0.518220 3.1:+54543684 MS.gene008306:CDS
CAACTTCCCGGCGCCGGCGA+CGG 0.518938 3.1:+54542553 MS.gene008306:CDS
ACTGGACAACGGAAATGGGT+TGG 0.521981 3.1:-54542610 None:intergenic
TTTGGTGGAGAGAGAGTGAG+AGG 0.525448 3.1:+54542962 MS.gene008306:CDS
GTTTGTACTAGGTTGTTGTC+GGG 0.528503 3.1:+54542886 MS.gene008306:CDS
AGAAACATGTTCCGCCGCAC+CGG 0.531078 3.1:+54542517 None:intergenic
AGATTGTTAAGGAACCAATT+GGG 0.542928 3.1:+54543295 MS.gene008306:CDS
AAAAGCTTCTTAATCACAAG+AGG 0.544112 3.1:-54543546 None:intergenic
GAGTTGTTGAAATTGTTGAA+TGG 0.546361 3.1:+54543738 MS.gene008306:CDS
AAACACCAACTTCCCGGCGC+CGG 0.546949 3.1:+54542547 MS.gene008306:CDS
CGGTGAATTAACGGTGGAGG+AGG 0.546975 3.1:+54542579 MS.gene008306:CDS
TTCAGGGTACGAGGTTAATG+AGG 0.547582 3.1:+54542864 MS.gene008306:CDS
TTGATTGAAGCAATTCAACT+TGG 0.549684 3.1:+54543648 MS.gene008306:CDS
AAAGATTGAAGGTGTTGTTG+AGG 0.552708 3.1:+54543263 MS.gene008306:CDS
TTGCTTGAAACAATTGTGGA+TGG 0.558779 3.1:+54543426 MS.gene008306:CDS
GAAACAGGTATACGAGGTGT+TGG 0.567045 3.1:-54542844 None:intergenic
CACCTCGTATACCTGTTTCA+GGG 0.570954 3.1:+54542848 MS.gene008306:CDS
TATGTTGCAAGTTGGGTGTG+AGG 0.588109 3.1:+54543692 MS.gene008306:CDS
GTGATTCGGTGTGATTTCGA+AGG 0.589458 3.1:-54542758 None:intergenic
TGATTGCAAGAAAGGGAAAG+AGG 0.594131 3.1:+54543497 MS.gene008306:CDS
GGGATATGTGAAAAGGCACT+TGG 0.611949 3.1:+54543456 MS.gene008306:CDS
CTCGATGCTGAATCTATCGT+AGG 0.614355 3.1:-54542680 None:intergenic
CGACGGTGAATTAACGGTGG+AGG 0.617998 3.1:+54542576 MS.gene008306:CDS
ACAGCAAAACTAGAAGCCAA+AGG 0.636688 3.1:-54543579 None:intergenic
TATACCTGTTTCAGGGTACG+AGG 0.666671 3.1:+54542855 MS.gene008306:CDS
TGGGTGTGAGGAGAAAACAA+AGG 0.673609 3.1:+54543704 MS.gene008306:CDS
AGTCTAGACTCACTGGACAA+CGG 0.681197 3.1:-54542621 None:intergenic
GTGTGAGGAGAAAACAAAGG+AGG 0.681227 3.1:+54543707 MS.gene008306:CDS
TACCCTGAAACAGGTATACG+AGG 0.702105 3.1:-54542850 None:intergenic
CGGCGACGGTGAATTAACGG+TGG 0.704914 3.1:+54542573 MS.gene008306:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAGATGGTAAAGATTT+AGG + chr3.1:54543186-54543205 MS.gene008306:CDS 20.0%
! TAAGAAAAATGATTCAGAGT+TGG + chr3.1:54542785-54542804 MS.gene008306:CDS 25.0%
!! ATTTTTCTTATGGTGTGATT+CGG - chr3.1:54542775-54542794 None:intergenic 25.0%
!! ATTTTTGCTTGAAACAATTG+TGG + chr3.1:54543422-54543441 MS.gene008306:CDS 25.0%
!! TTTTTCATGTGTTTCTTTTG+AGG + chr3.1:54543044-54543063 MS.gene008306:CDS 25.0%
!!! AATTTTTAAGCTGGTTTCAT+CGG + chr3.1:54543347-54543366 MS.gene008306:CDS 25.0%
!!! ACTCTGAATCATTTTTCTTA+TGG - chr3.1:54542785-54542804 None:intergenic 25.0%
!!! AGATTTTGGAGATTTTAACA+TGG + chr3.1:54543088-54543107 MS.gene008306:CDS 25.0%
!!! AGTGAAAGATTTGTTGAATT+GGG + chr3.1:54543390-54543409 MS.gene008306:CDS 25.0%
!!! TAGTGAAAGATTTGTTGAAT+TGG + chr3.1:54543389-54543408 MS.gene008306:CDS 25.0%
!!! TTTGACTACAATTTTTAAGC+TGG + chr3.1:54543338-54543357 MS.gene008306:CDS 25.0%
AAAAGCTTCTTAATCACAAG+AGG - chr3.1:54543549-54543568 None:intergenic 30.0%
AAGAGGGTTATAATTGGAAA+TGG + chr3.1:54542988-54543007 MS.gene008306:CDS 30.0%
AAGATTGTTAAGGAACCAAT+TGG + chr3.1:54543294-54543313 MS.gene008306:CDS 30.0%
AGAAAAAGGGATATGTGAAA+AGG + chr3.1:54543449-54543468 MS.gene008306:CDS 30.0%
AGATTGTTAAGGAACCAATT+GGG + chr3.1:54543295-54543314 MS.gene008306:CDS 30.0%
AGGAATAAGAGGGTTATAAT+TGG + chr3.1:54542982-54543001 MS.gene008306:CDS 30.0%
GAAGAGTTATCAAAGATTGA+AGG + chr3.1:54543252-54543271 MS.gene008306:CDS 30.0%
GAGTTGTTGAAATTGTTGAA+TGG + chr3.1:54543738-54543757 MS.gene008306:CDS 30.0%
GTTTATGTGATTGCAAGAAA+GGG + chr3.1:54543490-54543509 MS.gene008306:CDS 30.0%
TGGATTGTTAAAACCTCTTT+CGG + chr3.1:54543108-54543127 MS.gene008306:CDS 30.0%
TGTTTATGTGATTGCAAGAA+AGG + chr3.1:54543489-54543508 MS.gene008306:CDS 30.0%
TTGATTGAAGCAATTCAACT+TGG + chr3.1:54543648-54543667 MS.gene008306:CDS 30.0%
! AAAAAGTGTGTTGAGTTTGT+TGG + chr3.1:54542928-54542947 MS.gene008306:CDS 30.0%
! AAAAGTGTGTTGAGTTTGTT+GGG + chr3.1:54542929-54542948 MS.gene008306:CDS 30.0%
! AAAGTTTCTGATTCGTAACT+GGG - chr3.1:54542735-54542754 None:intergenic 30.0%
! TGTGATTAAGAAGCTTTTGA+GGG + chr3.1:54543551-54543570 MS.gene008306:CDS 30.0%
! TTGTGATTAAGAAGCTTTTG+AGG + chr3.1:54543550-54543569 MS.gene008306:CDS 30.0%
!! AAGATTTGTTGAATTGGGTT+TGG + chr3.1:54543395-54543414 MS.gene008306:CDS 30.0%
!!! TGTTTGGTTTGGTTTTTAGA+TGG + chr3.1:54543174-54543193 MS.gene008306:CDS 30.0%
ACAATTGTGGATGGAGAAAA+AGG + chr3.1:54543435-54543454 MS.gene008306:CDS 35.0%
AGGTTAATGAGGTTTGTACT+AGG + chr3.1:54542875-54542894 MS.gene008306:CDS 35.0%
CAATTGTGGATGGAGAAAAA+GGG + chr3.1:54543436-54543455 MS.gene008306:CDS 35.0%
CGTCTTTGAATTGTTTGGTT+TGG + chr3.1:54543163-54543182 MS.gene008306:CDS 35.0%
GCGTTGAGAATAGTTCTTAT+GGG + chr3.1:54542809-54542828 MS.gene008306:CDS 35.0%
GTCTTCGTCTTTGAATTGTT+TGG + chr3.1:54543158-54543177 MS.gene008306:CDS 35.0%
TGCGTTGAGAATAGTTCTTA+TGG + chr3.1:54542808-54542827 MS.gene008306:CDS 35.0%
TTGCTTGAAACAATTGTGGA+TGG + chr3.1:54543426-54543445 MS.gene008306:CDS 35.0%
TTGTTGGGAAGATTAAGGTT+TGG + chr3.1:54542944-54542963 MS.gene008306:CDS 35.0%
! ATAGATTCAGCATCGAGAAA+TGG + chr3.1:54542686-54542705 MS.gene008306:CDS 35.0%
! ATTCCTGTTGATAGAGTTAC+TGG - chr3.1:54542658-54542677 None:intergenic 35.0%
! GAAAGTTTCTGATTCGTAAC+TGG - chr3.1:54542736-54542755 None:intergenic 35.0%
! GGGTTTGGTTAAGATTGTTA+AGG + chr3.1:54543284-54543303 MS.gene008306:CDS 35.0%
! TGGTGCTTTTCAGAAATTGT+TGG + chr3.1:54543668-54543687 MS.gene008306:CDS 35.0%
! TGTTGGTTATGTTGCAAGTT+GGG + chr3.1:54543685-54543704 MS.gene008306:CDS 35.0%
! TTGTTGGTTATGTTGCAAGT+TGG + chr3.1:54543684-54543703 MS.gene008306:CDS 35.0%
!! AAAGATTGAAGGTGTTGTTG+AGG + chr3.1:54543263-54543282 MS.gene008306:CDS 35.0%
!! AAGATTGAAGGTGTTGTTGA+GGG + chr3.1:54543264-54543283 MS.gene008306:CDS 35.0%
!! TGAGTTTGTTGGGAAGATTA+AGG + chr3.1:54542939-54542958 MS.gene008306:CDS 35.0%
!!! TGAGGAACATGTTGTTGTTT+TGG + chr3.1:54543062-54543081 MS.gene008306:CDS 35.0%
!!! TGTTGTTTTGGAGGAGATTT+TGG + chr3.1:54543074-54543093 MS.gene008306:CDS 35.0%
AAAGCAAAACACCAACTTCC+CGG + chr3.1:54542541-54542560 MS.gene008306:CDS 40.0%
ACAGCAAAACTAGAAGCCAA+AGG - chr3.1:54543582-54543601 None:intergenic 40.0%
AGAGAGTGAGAGGAATAAGA+GGG + chr3.1:54542972-54542991 MS.gene008306:CDS 40.0%
GAAGATGCTTTGTGAGAAGA+AGG + chr3.1:54543614-54543633 MS.gene008306:CDS 40.0%
GATCCAGTAACTCTATCAAC+AGG + chr3.1:54542652-54542671 MS.gene008306:CDS 40.0%
GGTTTGTACTAGGTTGTTGT+CGG + chr3.1:54542885-54542904 MS.gene008306:CDS 40.0%
GTTTGTACTAGGTTGTTGTC+GGG + chr3.1:54542886-54542905 MS.gene008306:CDS 40.0%
TGATTGCAAGAAAGGGAAAG+AGG + chr3.1:54543497-54543516 MS.gene008306:CDS 40.0%
TTCATCAAGTCTAGACTCAC+TGG - chr3.1:54542631-54542650 None:intergenic 40.0%
TTGGGAAGATTAAGGTTTGG+TGG + chr3.1:54542947-54542966 MS.gene008306:CDS 40.0%
TTTGTAGCACTTGACCCAAT+TGG - chr3.1:54543312-54543331 None:intergenic 40.0%
! GTTGTCGGGTTGTAGAAATT+TGG + chr3.1:54542900-54542919 MS.gene008306:CDS 40.0%
!!! AAGTTGGTGTTTTGCTTTGC+CGG - chr3.1:54542539-54542558 None:intergenic 40.0%
!!! GGAACATGTTGTTGTTTTGG+AGG + chr3.1:54543065-54543084 MS.gene008306:CDS 40.0%
!!! GTTTTGCTGTTGGGATTGTT+AGG + chr3.1:54543592-54543611 MS.gene008306:CDS 40.0%
!!! TGAAAAGGCACTTGGTGTTT+TGG + chr3.1:54543464-54543483 MS.gene008306:CDS 40.0%
!!! TGGCTTCTAGTTTTGCTGTT+GGG + chr3.1:54543583-54543602 MS.gene008306:CDS 40.0%
!!! TTGGCTTCTAGTTTTGCTGT+TGG + chr3.1:54543582-54543601 MS.gene008306:CDS 40.0%
ACACCTCGTATACCTGTTTC+AGG + chr3.1:54542847-54542866 MS.gene008306:CDS 45.0%
ACTCACTGGACAACGGAAAT+GGG - chr3.1:54542617-54542636 None:intergenic 45.0%
AGTCTAGACTCACTGGACAA+CGG - chr3.1:54542624-54542643 None:intergenic 45.0%
ATCGAGAAATGGATCGAAGC+TGG + chr3.1:54542697-54542716 MS.gene008306:CDS 45.0%
CACCTCGTATACCTGTTTCA+GGG + chr3.1:54542848-54542867 MS.gene008306:CDS 45.0%
CTCGATGCTGAATCTATCGT+AGG - chr3.1:54542683-54542702 None:intergenic 45.0%
GAAACAGGTATACGAGGTGT+TGG - chr3.1:54542847-54542866 None:intergenic 45.0%
GAGAGAGTGAGAGGAATAAG+AGG + chr3.1:54542971-54542990 MS.gene008306:CDS 45.0%
GTGTGAGGAGAAAACAAAGG+AGG + chr3.1:54543707-54543726 MS.gene008306:CDS 45.0%
TACCCTGAAACAGGTATACG+AGG - chr3.1:54542853-54542872 None:intergenic 45.0%
TATACCTGTTTCAGGGTACG+AGG + chr3.1:54542855-54542874 MS.gene008306:CDS 45.0%
TGGGTGTGAGGAGAAAACAA+AGG + chr3.1:54543704-54543723 MS.gene008306:CDS 45.0%
TTAACCTCGTACCCTGAAAC+AGG - chr3.1:54542862-54542881 None:intergenic 45.0%
TTCAGGGTACGAGGTTAATG+AGG + chr3.1:54542864-54542883 MS.gene008306:CDS 45.0%
! CGTTTTCGAATCACCGAAAG+AGG - chr3.1:54543124-54543143 None:intergenic 45.0%
! TATGTTGCAAGTTGGGTGTG+AGG + chr3.1:54543692-54543711 MS.gene008306:CDS 45.0%
!! GGGATATGTGAAAAGGCACT+TGG + chr3.1:54543456-54543475 MS.gene008306:CDS 45.0%
!! GTGATTCGGTGTGATTTCGA+AGG - chr3.1:54542761-54542780 None:intergenic 45.0%
!! TGAAGGTGTTGTTGAGGGTT+TGG + chr3.1:54543269-54543288 MS.gene008306:CDS 45.0%
ACTGGACAACGGAAATGGGT+TGG - chr3.1:54542613-54542632 None:intergenic 50.0%
GACTCACTGGACAACGGAAA+TGG - chr3.1:54542618-54542637 None:intergenic 50.0%
TTTGGTGGAGAGAGAGTGAG+AGG + chr3.1:54542962-54542981 MS.gene008306:CDS 50.0%
! CTGGACAACGGAAATGGGTT+GGG - chr3.1:54542612-54542631 None:intergenic 50.0%
!!! GCTTTTGAGGGTGTCTCCTT+TGG + chr3.1:54543563-54543582 MS.gene008306:CDS 50.0%
CGACGGCGACGGTGAATTAA+CGG + chr3.1:54542570-54542589 MS.gene008306:CDS 55.0%
CGACGGTGAATTAACGGTGG+AGG + chr3.1:54542576-54542595 MS.gene008306:CDS 55.0%
CGGTGAATTAACGGTGGAGG+AGG + chr3.1:54542579-54542598 MS.gene008306:CDS 55.0%
! GGTGTTTTGCTTTGCCGGTG+CGG - chr3.1:54542534-54542553 None:intergenic 55.0%
AAACACCAACTTCCCGGCGC+CGG + chr3.1:54542547-54542566 MS.gene008306:CDS 60.0%
CGGCGACGGTGAATTAACGG+TGG + chr3.1:54542573-54542592 MS.gene008306:CDS 60.0%
TAATTCACCGTCGCCGTCGC+CGG - chr3.1:54542569-54542588 None:intergenic 60.0%
! GTTTTGCTTTGCCGGTGCGG+CGG - chr3.1:54542531-54542550 None:intergenic 60.0%
!! CAACTTCCCGGCGCCGGCGA+CGG + chr3.1:54542553-54542572 MS.gene008306:CDS 75.0%
!! CGTCGCCGGCGCCGGGAAGT+TGG - chr3.1:54542555-54542574 None:intergenic 80.0%
! ACCGTCGCCGTCGCCGGCGC+CGG - chr3.1:54542563-54542582 None:intergenic 85.0%
! CCGTCGCCGTCGCCGGCGCC+GGG - chr3.1:54542562-54542581 None:intergenic 90.0%
!!! CCCGGCGCCGGCGACGGCGA+CGG + chr3.1:54542559-54542578 MS.gene008306:CDS 90.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 54542523 54543821 54542523 ID=MS.gene008306
chr3.1 mRNA 54542523 54543821 54542523 ID=MS.gene008306.t1;Parent=MS.gene008306
chr3.1 exon 54542523 54543821 54542523 ID=MS.gene008306.t1.exon1;Parent=MS.gene008306.t1
chr3.1 CDS 54542523 54543821 54542523 ID=cds.MS.gene008306.t1;Parent=MS.gene008306.t1
Gene Sequence

>MS.gene008306

ATGTTCCGCCGCACCGGCAAAGCAAAACACCAACTTCCCGGCGCCGGCGACGGCGACGGTGAATTAACGGTGGAGGAGGTAACAATCCCAACCCATTTCCGTTGTCCAGTGAGTCTAGACTTGATGAAAGATCCAGTAACTCTATCAACAGGAATCACCTACGATAGATTCAGCATCGAGAAATGGATCGAAGCTGGAAATAAAACATGCCCAGTTACGAATCAGAAACTTTCAACCTTCGAAATCACACCGAATCACACCATAAGAAAAATGATTCAGAGTTGGTGCGTTGAGAATAGTTCTTATGGGATTGAGAGAATTCCAACACCTCGTATACCTGTTTCAGGGTACGAGGTTAATGAGGTTTGTACTAGGTTGTTGTCGGGTTGTAGAAATTTGGATGAGAAAAAGTGTGTTGAGTTTGTTGGGAAGATTAAGGTTTGGTGGAGAGAGAGTGAGAGGAATAAGAGGGTTATAATTGGAAATGGAGTTTCTTCTGTTCTTGCTAATGTTTTTGATTGTTTTTCATGTGTTTCTTTTGAGGAACATGTTGTTGTTTTGGAGGAGATTTTGGAGATTTTAACATGGATTGTTAAAACCTCTTTCGGTGATTCGAAAACGAAGATGTGTTTATCGTCTTCGTCTTTGAATTGTTTGGTTTGGTTTTTAGATGGTAAAGATTTAGGTGCAAGACAAAATGCTGTTTTGTTGCTTAAAGAAATGAATGTTGAAGAGTTATCAAAGATTGAAGGTGTTGTTGAGGGTTTGGTTAAGATTGTTAAGGAACCAATTGGGTCAAGTGCTACAAAAGCATGTTTGACTACAATTTTTAAGCTGGTTTCATCGGCGAAAAATAGAGATGAAATTAGTGAAAGATTTGTTGAATTGGGTTTGGTTTCATTTTTGCTTGAAACAATTGTGGATGGAGAAAAAGGGATATGTGAAAAGGCACTTGGTGTTTTGGATTGTTTATGTGATTGCAAGAAAGGGAAAGAGGTTGTTCAAACGAACGCTTTAGCTTTGCCTCTTGTGATTAAGAAGCTTTTGAGGGTGTCTCCTTTGGCTTCTAGTTTTGCTGTTGGGATTGTTAGGAAGATGCTTTGTGAGAAGAAGGAAGAAAGAGTTTTGATTGAAGCAATTCAACTTGGTGCTTTTCAGAAATTGTTGGTTATGTTGCAAGTTGGGTGTGAGGAGAAAACAAAGGAGGATACTACTGAGTTGTTGAAATTGTTGAATGGTTATAGAAGCAAAGCTGAATGTGTTGACAATTCATTAGATTTCAAGTATCTCAAGAACTAA
Protein sequence

>MS.gene008306.t1

MFRRTGKAKHQLPGAGDGDGELTVEEVTIPTHFRCPVSLDLMKDPVTLSTGITYDRFSIEKWIEAGNKTCPVTNQKLSTFEITPNHTIRKMIQSWCVENSSYGIERIPTPRIPVSGYEVNEVCTRLLSGCRNLDEKKCVEFVGKIKVWWRESERNKRVIIGNGVSSVLANVFDCFSCVSFEEHVVVLEEILEILTWIVKTSFGDSKTKMCLSSSSLNCLVWFLDGKDLGARQNAVLLLKEMNVEELSKIEGVVEGLVKIVKEPIGSSATKACLTTIFKLVSSAKNRDEISERFVELGLVSFLLETIVDGEKGICEKALGVLDCLCDCKKGKEVVQTNALALPLVIKKLLRVSPLASSFAVGIVRKMLCEKKEERVLIEAIQLGAFQKLLVMLQVGCEEKTKEDTTELLKLLNGYRSKAECVDNSLDFKYLKN