AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048677

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048677.t1 MTR_3g065080 96.811 439 14 0 1 439 1 439 0.0 861
MS.gene048677.t1 MTR_5g077510 64.334 443 149 8 1 438 1 439 0.0 545
MS.gene048677.t1 MTR_6g071340 47.733 419 201 7 33 438 2 415 2.56e-125 370
MS.gene048677.t1 MTR_5g048050 47.494 419 202 7 33 438 22 435 2.10e-123 365
MS.gene048677.t1 MTR_7g106340 35.225 423 247 11 32 436 4 417 2.09e-61 205
MS.gene048677.t1 MTR_2g087350 35.238 420 240 11 31 436 1 402 2.83e-60 202
MS.gene048677.t1 MTR_4g085720 32.767 412 247 10 34 434 12 404 1.11e-58 198
MS.gene048677.t1 MTR_1g056880 33.937 442 244 11 32 436 3 433 1.49e-58 198
MS.gene048677.t1 MTR_1g056870 33.484 442 246 11 32 436 3 433 5.32e-58 197
MS.gene048677.t1 MTR_1g056840 32.426 441 226 13 32 436 3 407 1.08e-50 177
MS.gene048677.t1 MTR_1g056910 31.830 399 246 13 31 416 1 386 1.57e-48 171
MS.gene048677.t1 MTR_4g051515 27.990 418 264 14 33 429 3 404 6.58e-41 150
MS.gene048677.t1 MTR_4g063800 26.379 417 266 13 25 426 244 634 1.38e-35 139
MS.gene048677.t1 MTR_5g083030 27.711 415 250 13 34 419 291 684 8.23e-34 134
MS.gene048677.t1 MTR_4g485520 25.908 413 265 12 24 418 89 478 1.91e-33 131
MS.gene048677.t1 MTR_3g095730 24.821 419 281 11 34 435 11 412 4.14e-33 129
MS.gene048677.t1 MTR_8g092870 26.014 419 278 12 34 435 8 411 4.98e-33 129
MS.gene048677.t1 MTR_4g485520 25.935 401 260 11 32 418 269 646 6.16e-33 131
MS.gene048677.t1 MTR_7g059405 26.799 403 243 17 36 419 264 633 6.95e-32 128
MS.gene048677.t1 MTR_5g015210 27.390 387 253 8 36 417 67 430 3.84e-29 119
MS.gene048677.t1 MTR_4g107010 29.365 378 227 13 3 360 247 604 2.05e-28 118
MS.gene048677.t1 MTR_3g096370 68.571 70 22 0 33 102 7 76 8.54e-27 111
MS.gene048677.t1 MTR_7g078330 24.085 328 220 8 9 324 240 550 1.57e-26 112
MS.gene048677.t1 MTR_1g017770 27.885 416 243 13 26 417 31 413 7.88e-26 109
MS.gene048677.t1 MTR_2g018010 28.125 320 180 14 34 328 290 584 9.89e-26 110
MS.gene048677.t1 MTR_4g028960 28.435 313 164 13 22 319 262 529 1.97e-25 109
MS.gene048677.t1 MTR_4g091880 24.359 390 238 13 35 420 4 340 7.85e-24 102
MS.gene048677.t1 MTR_1g094215 26.012 346 226 9 36 370 64 390 1.46e-23 102
MS.gene048677.t1 MTR_1g094215 26.361 349 221 11 36 370 247 573 1.69e-23 103
MS.gene048677.t1 MTR_3g085610 28.571 308 168 8 37 335 281 545 3.30e-23 103
MS.gene048677.t1 MTR_4g091880 24.685 397 256 13 35 420 4 368 3.53e-23 100
MS.gene048677.t1 MTR_3g078160 27.385 325 195 12 37 341 276 579 1.96e-21 97.4
MS.gene048677.t1 MTR_3g078160 27.385 325 195 12 37 341 276 579 2.32e-21 97.4
MS.gene048677.t1 MTR_7g116600 47.000 100 45 3 32 131 74 165 1.88e-20 93.6
MS.gene048677.t1 MTR_7g053260 52.174 69 33 0 34 102 76 144 5.96e-20 92.4
MS.gene048677.t1 MTR_3g078160 28.417 278 163 10 37 295 276 536 1.59e-19 91.3
MS.gene048677.t1 MTR_5g034440 45.133 113 46 4 40 141 284 391 2.30e-19 91.3
MS.gene048677.t1 MTR_1g069845 47.143 70 37 0 34 103 122 191 1.57e-18 88.6
MS.gene048677.t1 MTR_1g069845 47.143 70 37 0 34 103 243 312 2.47e-18 88.2
MS.gene048677.t1 MTR_1g069845 47.143 70 37 0 34 103 239 308 2.64e-18 88.2
MS.gene048677.t1 MTR_7g097020 44.595 74 41 0 34 107 239 312 3.28e-18 87.8
MS.gene048677.t1 MTR_2g011140 24.937 397 245 14 21 400 1 361 6.61e-18 85.5
MS.gene048677.t1 MTR_8g080280 39.560 91 49 1 37 127 283 367 6.97e-18 86.7
MS.gene048677.t1 MTR_8g077205 23.789 475 293 14 17 433 146 609 3.48e-17 84.3
MS.gene048677.t1 MTR_8g077205 23.789 475 293 14 17 433 258 721 4.62e-17 84.3
MS.gene048677.t1 MTR_1g079450 25.000 292 190 10 34 314 61 334 2.31e-16 81.3
MS.gene048677.t1 MTR_3g466220 41.892 74 43 0 34 107 231 304 4.33e-15 78.2
MS.gene048677.t1 MTR_3g466220 41.892 74 43 0 34 107 210 283 4.71e-15 78.2
MS.gene048677.t1 MTR_2g096850 45.070 71 39 0 32 102 585 655 1.20e-14 76.6
MS.gene048677.t1 MTR_2g096850 45.070 71 39 0 32 102 736 806 1.71e-14 76.3
MS.gene048677.t1 MTR_5g020570 38.372 86 49 2 32 117 269 350 1.99e-14 75.9
MS.gene048677.t1 MTR_5g020570 38.372 86 49 2 32 117 284 365 1.99e-14 75.9
MS.gene048677.t1 MTR_7g117890 26.459 257 158 7 40 292 259 488 4.27e-14 75.1
MS.gene048677.t1 MTR_3g096370 72.093 43 12 0 33 75 7 49 5.52e-14 72.8
MS.gene048677.t1 MTR_1g093965 46.875 64 31 1 49 112 1 61 5.94e-14 70.9
MS.gene048677.t1 MTR_1g093995 46.875 64 31 1 49 112 1 61 2.00e-13 69.3
MS.gene048677.t1 MTR_1g090320 40.625 96 48 4 31 118 504 598 5.52e-13 71.6
MS.gene048677.t1 MTR_1g090320 40.625 96 48 4 31 118 506 600 5.67e-13 71.6
MS.gene048677.t1 MTR_8g103227 38.636 88 48 2 22 108 386 468 1.22e-12 70.5
MS.gene048677.t1 MTR_5g032010 34.247 73 48 0 36 108 732 804 4.81e-12 68.6
MS.gene048677.t1 MTR_3g115670 24.399 291 172 10 40 316 34 290 5.13e-12 68.6
MS.gene048677.t1 MTR_1g100820 36.364 66 42 0 37 102 649 714 1.42e-11 67.0
MS.gene048677.t1 MTR_3g008270 43.939 66 36 1 37 102 731 795 1.43e-11 67.0
MS.gene048677.t1 MTR_1g100820 36.364 66 42 0 37 102 647 712 1.43e-11 67.0
MS.gene048677.t1 MTR_8g027140 41.176 68 40 0 40 107 263 330 9.32e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048677.t1 AT5G37490 45.894 414 207 8 33 438 28 432 1.09e-110 333
MS.gene048677.t1 AT1G66160 41.346 416 217 9 33 438 30 428 2.39e-101 309
MS.gene048677.t1 AT1G66160 38.942 416 211 10 33 438 30 412 1.47e-89 278
MS.gene048677.t1 AT3G02840 42.486 346 180 7 107 438 36 376 8.89e-77 244
MS.gene048677.t1 AT2G35930 35.154 421 248 8 27 436 3 409 4.62e-64 212
MS.gene048677.t1 AT3G19380 32.990 388 230 12 34 406 12 384 8.06e-54 185
MS.gene048677.t1 AT1G49780 33.417 398 241 11 34 418 12 398 8.86e-54 185
MS.gene048677.t1 AT3G11840 32.159 454 248 13 32 437 6 447 8.49e-47 167
MS.gene048677.t1 AT5G09800 28.101 395 257 12 35 418 10 388 8.28e-34 131
MS.gene048677.t1 AT2G28830 27.230 426 268 14 16 424 166 566 2.38e-33 132
MS.gene048677.t1 AT5G64660 27.206 408 258 13 34 418 8 399 4.58e-33 129
MS.gene048677.t1 AT3G46510 26.209 393 255 12 36 416 256 625 2.02e-32 130
MS.gene048677.t1 AT3G49810 25.700 393 264 6 36 423 64 433 1.66e-31 125
MS.gene048677.t1 AT1G23030 26.617 402 249 14 32 418 237 607 3.44e-31 126
MS.gene048677.t1 AT1G71020 26.877 413 255 14 26 418 85 470 5.80e-31 124
MS.gene048677.t1 AT1G71020 26.877 413 255 14 26 418 233 618 1.88e-30 124
MS.gene048677.t1 AT5G65920 25.570 395 266 6 36 425 60 431 1.06e-29 120
MS.gene048677.t1 AT5G65920 25.570 395 266 6 36 425 60 431 1.06e-29 120
MS.gene048677.t1 AT3G18710 26.238 404 261 12 34 418 10 395 3.73e-27 112
MS.gene048677.t1 AT3G54850 26.750 400 251 14 36 419 248 621 2.06e-26 112
MS.gene048677.t1 AT5G42340 24.242 264 182 6 34 293 288 537 3.97e-22 99.8
MS.gene048677.t1 AT3G07360 50.000 76 38 0 31 106 69 144 2.26e-20 93.6
MS.gene048677.t1 AT1G29340 44.037 109 53 3 34 134 303 411 7.46e-20 92.8
MS.gene048677.t1 AT1G10560 28.024 339 211 14 42 362 294 617 2.53e-19 91.3
MS.gene048677.t1 AT4G21350 25.962 312 198 10 36 339 5 291 6.14e-19 88.6
MS.gene048677.t1 AT5G01830 27.740 292 174 9 30 306 268 537 1.05e-18 89.4
MS.gene048677.t1 AT1G27910 41.758 91 47 1 37 127 280 364 1.19e-18 89.4
MS.gene048677.t1 AT1G60190 25.145 346 232 11 40 370 282 615 3.30e-18 87.8
MS.gene048677.t1 AT3G54790 49.315 73 37 0 34 106 200 272 5.47e-18 87.0
MS.gene048677.t1 AT3G54790 49.315 73 37 0 34 106 236 308 7.00e-18 86.7
MS.gene048677.t1 AT3G54790 49.315 73 37 0 34 106 236 308 7.00e-18 86.7
MS.gene048677.t1 AT1G67530 45.000 80 40 1 37 116 273 348 9.11e-18 85.9
MS.gene048677.t1 AT1G24330 40.659 91 48 1 37 127 276 360 1.13e-17 86.3
MS.gene048677.t1 AT1G24330 40.659 91 48 1 37 127 276 360 1.13e-17 86.3
MS.gene048677.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.18e-17 86.3
MS.gene048677.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.18e-17 86.3
MS.gene048677.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.18e-17 86.3
MS.gene048677.t1 AT5G67340 44.737 76 42 0 34 109 238 313 1.33e-16 82.8
MS.gene048677.t1 AT5G67340 44.737 76 42 0 34 109 238 313 1.33e-16 82.8
MS.gene048677.t1 AT3G52450 33.146 178 111 2 263 436 260 433 2.73e-16 80.9
MS.gene048677.t1 AT4G36550 40.278 72 43 0 35 106 218 289 3.99e-15 78.2
MS.gene048677.t1 AT1G01660 35.556 90 57 1 18 107 481 569 7.32e-15 77.0
MS.gene048677.t1 AT1G01660 35.556 90 57 1 18 107 480 568 7.57e-15 77.0
MS.gene048677.t1 AT2G45920 41.096 73 43 0 37 109 326 398 8.61e-15 76.3
MS.gene048677.t1 AT1G01680 38.571 70 43 0 40 109 237 306 1.02e-14 75.1
MS.gene048677.t1 AT2G23140 35.714 98 59 1 34 131 231 324 2.17e-14 75.9
MS.gene048677.t1 AT2G23140 35.714 98 59 1 34 131 228 321 2.24e-14 75.9
MS.gene048677.t1 AT2G23140 35.714 98 59 1 34 131 228 321 2.24e-14 75.9
MS.gene048677.t1 AT2G23140 37.000 100 57 2 34 131 39 134 3.94e-14 75.1
MS.gene048677.t1 AT2G23140 37.000 100 57 2 34 131 39 134 3.94e-14 75.1
MS.gene048677.t1 AT5G57035 40.909 66 39 0 37 102 709 774 1.05e-13 73.9
MS.gene048677.t1 AT1G56030 42.857 70 40 0 31 100 318 387 4.37e-13 70.9
MS.gene048677.t1 AT5G51270 44.286 70 38 1 37 106 736 804 6.19e-13 71.2
MS.gene048677.t1 AT5G51270 44.286 70 38 1 37 106 736 804 6.19e-13 71.2
MS.gene048677.t1 AT5G65500 40.299 67 40 0 36 102 715 781 6.82e-13 71.2
MS.gene048677.t1 AT5G65500 40.299 67 40 0 36 102 723 789 6.86e-13 71.2
MS.gene048677.t1 AT2G19410 43.939 66 36 1 37 102 712 776 7.98e-13 71.2
MS.gene048677.t1 AT2G19410 43.939 66 36 1 37 102 747 811 8.14e-13 71.2
MS.gene048677.t1 AT2G19410 43.939 66 36 1 37 102 726 790 8.19e-13 70.9
MS.gene048677.t1 AT3G61390 37.879 66 41 0 37 102 279 344 3.50e-12 68.2
MS.gene048677.t1 AT3G61390 37.879 66 41 0 37 102 354 419 4.66e-12 68.2
MS.gene048677.t1 AT3G49060 37.879 66 41 0 37 102 726 791 5.51e-12 68.6
MS.gene048677.t1 AT3G49060 37.879 66 41 0 37 102 736 801 5.69e-12 68.6
MS.gene048677.t1 AT3G49060 37.879 66 41 0 37 102 703 768 6.04e-12 68.2
MS.gene048677.t1 AT1G56040 40.625 64 38 0 37 100 354 417 1.20e-11 66.6
MS.gene048677.t1 AT4G25160 40.909 66 38 1 37 102 767 831 7.82e-11 64.7

Find 75 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATTGTTGATGGAGAAAA+AGG 0.166972 3.4:-66542820 MS.gene048677:CDS
GAAAGTTTCTGATTTGTAAC+TGG 0.278770 3.4:+66543522 None:intergenic
TAGTGAAAAGTTTGTTGAAT+TGG 0.291875 3.4:-66542866 MS.gene048677:CDS
TGAGGAACATGTTGTTGTTT+TGG 0.298411 3.4:-66543193 MS.gene048677:CDS
ATTCCTGTTGATAGAGTTAC+TGG 0.298592 3.4:+66543600 None:intergenic
CATCTTTGAATTGTTTAGTT+TGG 0.299040 3.4:-66543092 MS.gene048677:CDS
AGGAATAAGAGGGTTATAAT+AGG 0.322167 3.4:-66543273 MS.gene048677:CDS
AGTGAAAAGTTTGTTGAATT+GGG 0.337948 3.4:-66542865 MS.gene048677:CDS
TGAGTTTGTTGGAAAGATTA+AGG 0.348264 3.4:-66543316 MS.gene048677:CDS
TTGCTTGAAACAATTGTTGA+TGG 0.366375 3.4:-66542829 MS.gene048677:CDS
GTTGTCGGGTTGTAGAAATT+TGG 0.371187 3.4:-66543355 MS.gene048677:CDS
TGTGTTGAAAATAGTTCTTA+TGG 0.375679 3.4:-66543447 MS.gene048677:CDS
TGAAGGTGTTGTTGAGGGTT+TGG 0.377823 3.4:-66542986 MS.gene048677:CDS
GAGAGAGTGAGAGGAATAAG+AGG 0.381694 3.4:-66543284 MS.gene048677:CDS
AAGATTGTTAAGGAACCAAT+TGG 0.381968 3.4:-66542961 MS.gene048677:CDS
GACTCACCGGACAACGGAAA+TGG 0.385326 3.4:+66543640 None:intergenic
GAAGATGCTTTGTGAGAAGA+AGG 0.393306 3.4:-66542641 MS.gene048677:CDS
TAATTCGCCGTCGCCGGCGC+CGG 0.395081 3.4:+66543689 None:intergenic
CGCCGGCGACGGCGAATTAA+CGG 0.401684 3.4:-66543685 MS.gene048677:CDS
AAGAGGGTTATAATAGGAAA+TGG 0.402171 3.4:-66543267 MS.gene048677:CDS
TTTGTAGCACTTGACCCAAT+TGG 0.406484 3.4:+66542946 None:intergenic
CCCAACCCATTTCCGTTGTC+CGG 0.411042 3.4:-66543646 MS.gene048677:CDS
CACCGTTAATTCGCCGTCGC+CGG 0.412333 3.4:+66543683 None:intergenic
TGTTTATGTGATTGTAAGAA+AGG 0.414472 3.4:-66542766 MS.gene048677:CDS
TGTTGGTTATGTTGCAAGTT+GGG 0.414797 3.4:-66542570 MS.gene048677:CDS
AAAGCAAAACACCAACTTCC+CGG 0.416680 3.4:-66543708 MS.gene048677:CDS
AATTCGCCGTCGCCGGCGCC+GGG 0.439130 3.4:+66543690 None:intergenic
GTGTTGAAAATAGTTCTTAT+GGG 0.440410 3.4:-66543446 MS.gene048677:CDS
TGAAAAGGCACTAGGTGTAT+TGG 0.441241 3.4:-66542791 MS.gene048677:CDS
TTAACCTCGTACGCTGAAAC+AGG 0.447425 3.4:+66543396 None:intergenic
CGTCGCCGGCGCCGGGAAGT+TGG 0.449959 3.4:+66543697 None:intergenic
TTGGAAAGATTAAGGTTTGG+TGG 0.456739 3.4:-66543308 MS.gene048677:CDS
GGTTTGTACTAGGTTGTTGT+CGG 0.456977 3.4:-66543370 MS.gene048677:CDS
CCGGACAACGGAAATGGGTT+GGG 0.459441 3.4:+66543646 None:intergenic
GATCCAGTAACTCTATCAAC+AGG 0.465887 3.4:-66543603 MS.gene048677:CDS
GGGTTTGGTGAAGATTGTTA+AGG 0.467901 3.4:-66542971 MS.gene048677:CDS
AGAATTGAAGGTGTTGTTGA+GGG 0.472391 3.4:-66542991 MS.gene048677:CDS
ATAGATTCAGCATCGAGAAA+TGG 0.477438 3.4:-66543569 MS.gene048677:CDS
AGGTTAATGAGGTTTGTACT+AGG 0.482538 3.4:-66543380 MS.gene048677:CDS
ACTCACCGGACAACGGAAAT+GGG 0.483936 3.4:+66543641 None:intergenic
GAAGAGTTATCAAGAATTGA+AGG 0.485697 3.4:-66543003 MS.gene048677:CDS
ACCGGACAACGGAAATGGGT+TGG 0.495073 3.4:+66543645 None:intergenic
GTTTATGTGATTGTAAGAAA+GGG 0.496421 3.4:-66542765 MS.gene048677:CDS
TTGTTGGAAAGATTAAGGTT+TGG 0.496940 3.4:-66543311 MS.gene048677:CDS
AGAGAGTGAGAGGAATAAGA+GGG 0.507848 3.4:-66543283 MS.gene048677:CDS
ATCGAGAAATGGATCGAAGC+TGG 0.513343 3.4:-66543558 MS.gene048677:CDS
TTGTTGGTTATGTTGCAAGT+TGG 0.518220 3.4:-66542571 MS.gene048677:CDS
CAACTTCCCGGCGCCGGCGA+CGG 0.518938 3.4:-66543696 MS.gene048677:CDS
TTTGGTGGAGAGAGAGTGAG+AGG 0.525448 3.4:-66543293 MS.gene048677:CDS
GTTTGTACTAGGTTGTTGTC+GGG 0.528503 3.4:-66543369 MS.gene048677:CDS
AAAGTTTCTGATTTGTAACT+GGG 0.529983 3.4:+66543523 None:intergenic
AGAAACATGTTCCGCCGCAC+CGG 0.531078 3.4:-66543732 MS.gene048677:CDS
AAAAGCTTCTTAATCACAAG+AGG 0.544112 3.4:+66542709 None:intergenic
GAGTTGTTGAAATTGTTGAA+TGG 0.546361 3.4:-66542517 MS.gene048677:CDS
AAACACCAACTTCCCGGCGC+CGG 0.546949 3.4:-66543702 MS.gene048677:CDS
TTGATTGAAGCAATTCAACT+TGG 0.549684 3.4:-66542607 MS.gene048677:CDS
AAGAATTGAAGGTGTTGTTG+AGG 0.552708 3.4:-66542992 MS.gene048677:CDS
TTCAGCGTACGAGGTTAATG+AGG 0.552963 3.4:-66543391 MS.gene048677:CDS
GAAACAGGTATACGAGGTGT+TGG 0.554990 3.4:+66543411 None:intergenic
GTGATTTGGTGTGATTTCGA+AGG 0.579587 3.4:+66543497 None:intergenic
TTCATCAAGTCTAGACTCAC+CGG 0.580014 3.4:+66543627 None:intergenic
AGATTGTTAAGGAACCAATT+GGG 0.580879 3.4:-66542960 MS.gene048677:CDS
CGACGGCGAATTAACGGTGG+AGG 0.584194 3.4:-66543679 MS.gene048677:CDS
TATGTTGCAAGTTGGGTGTG+AGG 0.588109 3.4:-66542563 MS.gene048677:CDS
AAGGGAAAGAGGTTGTTCAA+AGG 0.593560 3.4:-66542747 MS.gene048677:CDS
TGATTGTAAGAAAGGGAAAG+AGG 0.594131 3.4:-66542758 MS.gene048677:CDS
TATACCTGTTTCAGCGTACG+AGG 0.604860 3.4:-66543400 MS.gene048677:CDS
CTCGATGCTGAATCTATCGT+AGG 0.614355 3.4:+66543575 None:intergenic
GGGATATGTGAAAAGGCACT+AGG 0.622666 3.4:-66542799 MS.gene048677:CDS
ACAGCAAAACTAGAAGCCAA+AGG 0.636688 3.4:+66542676 None:intergenic
AGTCTAGACTCACCGGACAA+CGG 0.656680 3.4:+66543634 None:intergenic
TGGGTGTGAGGAGAAAACAA+AGG 0.673609 3.4:-66542551 MS.gene048677:CDS
GTGTGAGGAGAAAACAAAGG+AGG 0.681227 3.4:-66542548 MS.gene048677:CDS
TACGCTGAAACAGGTATACG+AGG 0.716974 3.4:+66543405 None:intergenic
CGGCGACGGCGAATTAACGG+TGG 0.722796 3.4:-66543682 MS.gene048677:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTAGATGGTAAAGATTT+AGG - chr3.4:66543140-66543159 MS.gene048677:CDS 20.0%
! CATCTTTGAATTGTTTAGTT+TGG - chr3.4:66543117-66543136 MS.gene048677:CDS 25.0%
! GTGTTGAAAATAGTTCTTAT+GGG - chr3.4:66542763-66542782 MS.gene048677:CDS 25.0%
! GTTTATGTGATTGTAAGAAA+GGG - chr3.4:66543444-66543463 MS.gene048677:CDS 25.0%
! TAAGAAAAATGATTCAGAGT+TGG - chr3.4:66542739-66542758 MS.gene048677:CDS 25.0%
! TGTGTTGAAAATAGTTCTTA+TGG - chr3.4:66542762-66542781 MS.gene048677:CDS 25.0%
! TGTTTATGTGATTGTAAGAA+AGG - chr3.4:66543443-66543462 MS.gene048677:CDS 25.0%
!! AAAGTTTCTGATTTGTAACT+GGG + chr3.4:66542689-66542708 None:intergenic 25.0%
!! ATTTTTCTTATGGTGTGATT+TGG + chr3.4:66542729-66542748 None:intergenic 25.0%
!! TTTTTCATGTGTTTCTTTTG+AGG - chr3.4:66542998-66543017 MS.gene048677:CDS 25.0%
!!! AATTTTCAAGTTGGTTTCAT+CGG - chr3.4:66543301-66543320 MS.gene048677:CDS 25.0%
!!! ACTCTGAATCATTTTTCTTA+TGG + chr3.4:66542739-66542758 None:intergenic 25.0%
!!! AGATTTTGGAGATTTTAACA+TGG - chr3.4:66543042-66543061 MS.gene048677:CDS 25.0%
!!! AGTGAAAAGTTTGTTGAATT+GGG - chr3.4:66543344-66543363 MS.gene048677:CDS 25.0%
!!! TAGTGAAAAGTTTGTTGAAT+TGG - chr3.4:66543343-66543362 MS.gene048677:CDS 25.0%
!!! TGTTTAGTTTGGTTTTTAGA+TGG - chr3.4:66543128-66543147 MS.gene048677:CDS 25.0%
!!! TTTGACTACAATTTTCAAGT+TGG - chr3.4:66543292-66543311 MS.gene048677:CDS 25.0%
AAAAGCTTCTTAATCACAAG+AGG + chr3.4:66543503-66543522 None:intergenic 30.0%
AAGAGGGTTATAATAGGAAA+TGG - chr3.4:66542942-66542961 MS.gene048677:CDS 30.0%
AAGATTGTTAAGGAACCAAT+TGG - chr3.4:66543248-66543267 MS.gene048677:CDS 30.0%
AGAAAAAGGGATATGTGAAA+AGG - chr3.4:66543403-66543422 MS.gene048677:CDS 30.0%
AGATTGTTAAGGAACCAATT+GGG - chr3.4:66543249-66543268 MS.gene048677:CDS 30.0%
AGGAATAAGAGGGTTATAAT+AGG - chr3.4:66542936-66542955 MS.gene048677:CDS 30.0%
GAAGAGTTATCAAGAATTGA+AGG - chr3.4:66543206-66543225 MS.gene048677:CDS 30.0%
GAGTTGTTGAAATTGTTGAA+TGG - chr3.4:66543692-66543711 MS.gene048677:CDS 30.0%
TTGATTGAAGCAATTCAACT+TGG - chr3.4:66543602-66543621 MS.gene048677:CDS 30.0%
TTGCTTGAAACAATTGTTGA+TGG - chr3.4:66543380-66543399 MS.gene048677:CDS 30.0%
TTGTTGGAAAGATTAAGGTT+TGG - chr3.4:66542898-66542917 MS.gene048677:CDS 30.0%
! AAAAAGTGTGTTGAGTTTGT+TGG - chr3.4:66542882-66542901 MS.gene048677:CDS 30.0%
! ACAATTGTTGATGGAGAAAA+AGG - chr3.4:66543389-66543408 MS.gene048677:CDS 30.0%
! CAATTGTTGATGGAGAAAAA+GGG - chr3.4:66543390-66543409 MS.gene048677:CDS 30.0%
! GAAAGTTTCTGATTTGTAAC+TGG + chr3.4:66542690-66542709 None:intergenic 30.0%
! TGTGATTAAGAAGCTTTTGA+GGG - chr3.4:66543505-66543524 MS.gene048677:CDS 30.0%
! TTGTGATTAAGAAGCTTTTG+AGG - chr3.4:66543504-66543523 MS.gene048677:CDS 30.0%
!! TGAGTTTGTTGGAAAGATTA+AGG - chr3.4:66542893-66542912 MS.gene048677:CDS 30.0%
!!! AGTTTGTTCTGTTTTAGCTA+CGG - chr3.4:66542965-66542984 MS.gene048677:CDS 30.0%
AGGTTAATGAGGTTTGTACT+AGG - chr3.4:66542829-66542848 MS.gene048677:CDS 35.0%
TGATTGTAAGAAAGGGAAAG+AGG - chr3.4:66543451-66543470 MS.gene048677:CDS 35.0%
TTGGAAAGATTAAGGTTTGG+TGG - chr3.4:66542901-66542920 MS.gene048677:CDS 35.0%
! ATAGATTCAGCATCGAGAAA+TGG - chr3.4:66542640-66542659 MS.gene048677:CDS 35.0%
! ATTCCTGTTGATAGAGTTAC+TGG + chr3.4:66542612-66542631 None:intergenic 35.0%
! TGGTGCTTTTCAGAAATTGT+TGG - chr3.4:66543622-66543641 MS.gene048677:CDS 35.0%
! TGTTGGTTATGTTGCAAGTT+GGG - chr3.4:66543639-66543658 MS.gene048677:CDS 35.0%
! TTGTTGGTTATGTTGCAAGT+TGG - chr3.4:66543638-66543657 MS.gene048677:CDS 35.0%
!! AAGAATTGAAGGTGTTGTTG+AGG - chr3.4:66543217-66543236 MS.gene048677:CDS 35.0%
!! AGAATTGAAGGTGTTGTTGA+GGG - chr3.4:66543218-66543237 MS.gene048677:CDS 35.0%
!!! TGAGGAACATGTTGTTGTTT+TGG - chr3.4:66543016-66543035 MS.gene048677:CDS 35.0%
!!! TGTTGTTTTGGAGGAGATTT+TGG - chr3.4:66543028-66543047 MS.gene048677:CDS 35.0%
AAAGCAAAACACCAACTTCC+CGG - chr3.4:66542501-66542520 MS.gene048677:CDS 40.0%
AAGGGAAAGAGGTTGTTCAA+AGG - chr3.4:66543462-66543481 MS.gene048677:CDS 40.0%
ACAGCAAAACTAGAAGCCAA+AGG + chr3.4:66543536-66543555 None:intergenic 40.0%
AGAGAGTGAGAGGAATAAGA+GGG - chr3.4:66542926-66542945 MS.gene048677:CDS 40.0%
GAAGATGCTTTGTGAGAAGA+AGG - chr3.4:66543568-66543587 MS.gene048677:CDS 40.0%
GATCCAGTAACTCTATCAAC+AGG - chr3.4:66542606-66542625 MS.gene048677:CDS 40.0%
GGTTTGTACTAGGTTGTTGT+CGG - chr3.4:66542839-66542858 MS.gene048677:CDS 40.0%
GTTTGTACTAGGTTGTTGTC+GGG - chr3.4:66542840-66542859 MS.gene048677:CDS 40.0%
TTCATCAAGTCTAGACTCAC+CGG + chr3.4:66542585-66542604 None:intergenic 40.0%
TTTGTAGCACTTGACCCAAT+TGG + chr3.4:66543266-66543285 None:intergenic 40.0%
! CGTTTTCGAATCGCTAAAAG+AGG + chr3.4:66543078-66543097 None:intergenic 40.0%
! GTTGTCGGGTTGTAGAAATT+TGG - chr3.4:66542854-66542873 MS.gene048677:CDS 40.0%
!! GGGTTTGGTGAAGATTGTTA+AGG - chr3.4:66543238-66543257 MS.gene048677:CDS 40.0%
!! GTGATTTGGTGTGATTTCGA+AGG + chr3.4:66542715-66542734 None:intergenic 40.0%
!! TGAAAAGGCACTAGGTGTAT+TGG - chr3.4:66543418-66543437 MS.gene048677:CDS 40.0%
!!! AAGTTGGTGTTTTGCTTTGC+CGG + chr3.4:66542499-66542518 None:intergenic 40.0%
!!! GGAACATGTTGTTGTTTTGG+AGG - chr3.4:66543019-66543038 MS.gene048677:CDS 40.0%
!!! GTTTTGCTGTTGGGATTGTT+AGG - chr3.4:66543546-66543565 MS.gene048677:CDS 40.0%
!!! TGGCTTCTAGTTTTGCTGTT+GGG - chr3.4:66543537-66543556 MS.gene048677:CDS 40.0%
!!! TTGGCTTCTAGTTTTGCTGT+TGG - chr3.4:66543536-66543555 MS.gene048677:CDS 40.0%
ATCGAGAAATGGATCGAAGC+TGG - chr3.4:66542651-66542670 MS.gene048677:CDS 45.0%
CTCGATGCTGAATCTATCGT+AGG + chr3.4:66542637-66542656 None:intergenic 45.0%
GAAACAGGTATACGAGGTGT+TGG + chr3.4:66542801-66542820 None:intergenic 45.0%
GAGAGAGTGAGAGGAATAAG+AGG - chr3.4:66542925-66542944 MS.gene048677:CDS 45.0%
GTGTGAGGAGAAAACAAAGG+AGG - chr3.4:66543661-66543680 MS.gene048677:CDS 45.0%
TACGCTGAAACAGGTATACG+AGG + chr3.4:66542807-66542826 None:intergenic 45.0%
TATACCTGTTTCAGCGTACG+AGG - chr3.4:66542809-66542828 MS.gene048677:CDS 45.0%
TGGGTGTGAGGAGAAAACAA+AGG - chr3.4:66543658-66543677 MS.gene048677:CDS 45.0%
TTAACCTCGTACGCTGAAAC+AGG + chr3.4:66542816-66542835 None:intergenic 45.0%
TTCAGCGTACGAGGTTAATG+AGG - chr3.4:66542818-66542837 MS.gene048677:CDS 45.0%
! TATGTTGCAAGTTGGGTGTG+AGG - chr3.4:66543646-66543665 MS.gene048677:CDS 45.0%
!! GGGATATGTGAAAAGGCACT+AGG - chr3.4:66543410-66543429 MS.gene048677:CDS 45.0%
!! TGAAGGTGTTGTTGAGGGTT+TGG - chr3.4:66543223-66543242 MS.gene048677:CDS 45.0%
ACTCACCGGACAACGGAAAT+GGG + chr3.4:66542571-66542590 None:intergenic 50.0%
AGTCTAGACTCACCGGACAA+CGG + chr3.4:66542578-66542597 None:intergenic 50.0%
TTTGGTGGAGAGAGAGTGAG+AGG - chr3.4:66542916-66542935 MS.gene048677:CDS 50.0%
!!! GCTTTTGAGGGTGTCTCCTT+TGG - chr3.4:66543517-66543536 MS.gene048677:CDS 50.0%
ACCGGACAACGGAAATGGGT+TGG + chr3.4:66542567-66542586 None:intergenic 55.0%
AGAAACATGTTCCGCCGCAC+CGG - chr3.4:66542477-66542496 MS.gene048677:CDS 55.0%
CCCAACCCATTTCCGTTGTC+CGG - chr3.4:66542563-66542582 MS.gene048677:CDS 55.0%
GACTCACCGGACAACGGAAA+TGG + chr3.4:66542572-66542591 None:intergenic 55.0%
! CCGGACAACGGAAATGGGTT+GGG + chr3.4:66542566-66542585 None:intergenic 55.0%
! GGTGTTTTGCTTTGCCGGTG+CGG + chr3.4:66542494-66542513 None:intergenic 55.0%
AAACACCAACTTCCCGGCGC+CGG - chr3.4:66542507-66542526 MS.gene048677:CDS 60.0%
CACCGTTAATTCGCCGTCGC+CGG + chr3.4:66542529-66542548 None:intergenic 60.0%
CGACGGCGAATTAACGGTGG+AGG - chr3.4:66542530-66542549 MS.gene048677:CDS 60.0%
! GTTTTGCTTTGCCGGTGCGG+CGG + chr3.4:66542491-66542510 None:intergenic 60.0%
CGCCGGCGACGGCGAATTAA+CGG - chr3.4:66542524-66542543 MS.gene048677:CDS 65.0%
CGGCGACGGCGAATTAACGG+TGG - chr3.4:66542527-66542546 MS.gene048677:CDS 65.0%
TAATTCGCCGTCGCCGGCGC+CGG + chr3.4:66542523-66542542 None:intergenic 70.0%
AATTCGCCGTCGCCGGCGCC+GGG + chr3.4:66542522-66542541 None:intergenic 75.0%
!! CAACTTCCCGGCGCCGGCGA+CGG - chr3.4:66542513-66542532 MS.gene048677:CDS 75.0%
!! CGTCGCCGGCGCCGGGAAGT+TGG + chr3.4:66542515-66542534 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 66542456 66543775 66542456 ID=MS.gene048677
chr3.4 mRNA 66542456 66543775 66542456 ID=MS.gene048677.t1;Parent=MS.gene048677
chr3.4 exon 66542456 66543775 66542456 ID=MS.gene048677.t1.exon1;Parent=MS.gene048677.t1
chr3.4 CDS 66542456 66543775 66542456 ID=cds.MS.gene048677.t1;Parent=MS.gene048677.t1
Gene Sequence

>MS.gene048677

ATGGTTTTATCATGGACAAAAAGAAACATGTTCCGCCGCACCGGCAAAGCAAAACACCAACTTCCCGGCGCCGGCGACGGCGAATTAACGGTGGAGGAGATAACAATCCCAACCCATTTCCGTTGTCCGGTGAGTCTAGACTTGATGAAAGATCCAGTAACTCTATCAACAGGAATCACCTACGATAGATTCAGCATCGAGAAATGGATCGAAGCTGGAAACAAAACTTGCCCAGTTACAAATCAGAAACTTTCAACCTTCGAAATCACACCAAATCACACCATAAGAAAAATGATTCAGAGTTGGTGTGTTGAAAATAGTTCTTATGGGATTGAGAGAATTCCAACACCTCGTATACCTGTTTCAGCGTACGAGGTTAATGAGGTTTGTACTAGGTTGTTGTCGGGTTGTAGAAATTTGGATGAGAAAAAGTGTGTTGAGTTTGTTGGAAAGATTAAGGTTTGGTGGAGAGAGAGTGAGAGGAATAAGAGGGTTATAATAGGAAATGGAGTTTGTTCTGTTTTAGCTACGGTTTTTGATTGTTTTTCATGTGTTTCTTTTGAGGAACATGTTGTTGTTTTGGAGGAGATTTTGGAGATTTTAACATGGATTGTTAAAACCTCTTTTAGCGATTCGAAAACGAAGATGTGTTTATCGTCTTCATCTTTGAATTGTTTAGTTTGGTTTTTAGATGGTAAAGATTTAGGTGCAAGACAAAATGCTGTTTTGTTGCTTAAAGAAATGAATGTTGAAGAGTTATCAAGAATTGAAGGTGTTGTTGAGGGTTTGGTGAAGATTGTTAAGGAACCAATTGGGTCAAGTGCTACAAAAGCATGTTTGACTACAATTTTCAAGTTGGTTTCATCGGCGAAAAATAGAGATGAGATTAGTGAAAAGTTTGTTGAATTGGGTTTGATTTCATTTTTGCTTGAAACAATTGTTGATGGAGAAAAAGGGATATGTGAAAAGGCACTAGGTGTATTGGATTGTTTATGTGATTGTAAGAAAGGGAAAGAGGTTGTTCAAAGGAACGCTTTAGCTTTGCCTCTTGTGATTAAGAAGCTTTTGAGGGTGTCTCCTTTGGCTTCTAGTTTTGCTGTTGGGATTGTTAGGAAGATGCTTTGTGAGAAGAAGGAAGAAAGAGTTTTGATTGAAGCAATTCAACTTGGTGCTTTTCAGAAATTGTTGGTTATGTTGCAAGTTGGGTGTGAGGAGAAAACAAAGGAGGATACTACTGAGTTGTTGAAATTGTTGAATGGTTATAGAAGCAAAGCTGAATGTGTTGACAATTCATTAGATTTCAAGTATCTCAAGAACTAA
Protein sequence

>MS.gene048677.t1

MVLSWTKRNMFRRTGKAKHQLPGAGDGELTVEEITIPTHFRCPVSLDLMKDPVTLSTGITYDRFSIEKWIEAGNKTCPVTNQKLSTFEITPNHTIRKMIQSWCVENSSYGIERIPTPRIPVSAYEVNEVCTRLLSGCRNLDEKKCVEFVGKIKVWWRESERNKRVIIGNGVCSVLATVFDCFSCVSFEEHVVVLEEILEILTWIVKTSFSDSKTKMCLSSSSLNCLVWFLDGKDLGARQNAVLLLKEMNVEELSRIEGVVEGLVKIVKEPIGSSATKACLTTIFKLVSSAKNRDEISEKFVELGLISFLLETIVDGEKGICEKALGVLDCLCDCKKGKEVVQRNALALPLVIKKLLRVSPLASSFAVGIVRKMLCEKKEERVLIEAIQLGAFQKLLVMLQVGCEEKTKEDTTELLKLLNGYRSKAECVDNSLDFKYLKN