AlfalfaGEDB | MS.gene72611

M. sativa cultivar XinJiangDaYe / MS.gene72611

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72611.t1	RHN52322.1	96.8	438	14	0	1	438	1	438	2.40E-244	854.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72611.t1	Q5PNY6	43.9	442	229	6	5	438	5	435	7.8e-95	349.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72611.t1	A0A396HG48	96.8	438	14	0	1	438	1	438	1.7e-244	854.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04998	MS.gene72611	0.876753	1.09E-68	-1.69E-46
MS.gene050414	MS.gene72611	0.813006	3.18E-51	-1.69E-46
MS.gene051226	MS.gene72611	0.843033	1.80E-58	-1.69E-46
MS.gene051959	MS.gene72611	0.805735	1.16E-49	-1.69E-46
MS.gene052016	MS.gene72611	0.824367	8.39E-54	-1.69E-46
MS.gene052533	MS.gene72611	0.800933	1.14E-48	-1.69E-46
MS.gene053298	MS.gene72611	0.80891	2.45E-50	-1.69E-46
MS.gene054617	MS.gene72611	0.803621	3.20E-49	-1.69E-46
MS.gene054892	MS.gene72611	0.870676	1.22E-66	-1.69E-46
MS.gene054893	MS.gene72611	0.86919	3.73E-66	-1.69E-46
MS.gene056677	MS.gene72611	0.80178	7.66E-49	-1.69E-46
MS.gene057093	MS.gene72611	0.82814	1.06E-54	-1.69E-46
MS.gene057622	MS.gene72611	0.851175	1.05E-60	-1.69E-46
MS.gene058159	MS.gene72611	0.802359	5.83E-49	-1.69E-46
MS.gene059170	MS.gene72611	0.805936	1.05E-49	-1.69E-46
MS.gene05952	MS.gene72611	0.824096	9.71E-54	-1.69E-46
MS.gene059774	MS.gene72611	0.811024	8.61E-51	-1.69E-46
MS.gene06023	MS.gene72611	0.841541	4.46E-58	-1.69E-46
MS.gene060259	MS.gene72611	0.833437	5.33E-56	-1.69E-46
MS.gene060412	MS.gene72611	0.827794	1.28E-54	-1.69E-46
MS.gene060413	MS.gene72611	0.81775	2.81E-52	-1.69E-46
MS.gene060697	MS.gene72611	0.808306	3.30E-50	-1.69E-46
MS.gene060964	MS.gene72611	0.809198	2.13E-50	-1.69E-46
MS.gene061047	MS.gene72611	0.835785	1.37E-56	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene022190	MS.gene72611	PPI
MS.gene061546	MS.gene72611	PPI
MS.gene064492	MS.gene72611	PPI
MS.gene017373	MS.gene72611	PPI
MS.gene042912	MS.gene72611	PPI
MS.gene26489	MS.gene72611	PPI
MS.gene066129	MS.gene72611	PPI
MS.gene93972	MS.gene72611	PPI
MS.gene034751	MS.gene72611	PPI
MS.gene034753	MS.gene72611	PPI
MS.gene008306	MS.gene72611	PPI
MS.gene41032	MS.gene72611	PPI
MS.gene45454	MS.gene72611	PPI
MS.gene41033	MS.gene72611	PPI
MS.gene04998	MS.gene72611	PPI
MS.gene048677	MS.gene72611	PPI
MS.gene26139	MS.gene72611	PPI
MS.gene025667	MS.gene72611	PPI
MS.gene007121	MS.gene72611	PPI
MS.gene061543	MS.gene72611	PPI
MS.gene066130	MS.gene72611	PPI
MS.gene45456	MS.gene72611	PPI
MS.gene45455	MS.gene72611	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72611.t1	MTR_6g071340	96.890	418	13	0	21	438	1	418	0.0	836
MS.gene72611.t1	MTR_5g048050	95.662	438	19	0	1	438	1	438	0.0	807
MS.gene72611.t1	MTR_5g077510	49.011	455	202	11	1	438	1	442	1.20e-138	404
MS.gene72611.t1	MTR_3g065080	46.341	451	213	8	1	435	1	438	2.78e-128	378
MS.gene72611.t1	MTR_2g087350	35.149	404	229	11	22	415	3	383	8.26e-56	190
MS.gene72611.t1	MTR_1g056870	31.542	428	246	11	20	415	2	414	1.33e-55	190
MS.gene72611.t1	MTR_1g056880	31.308	428	247	11	20	415	2	414	2.91e-55	189
MS.gene72611.t1	MTR_7g106340	29.782	413	252	11	22	415	5	398	1.83e-50	176
MS.gene72611.t1	MTR_4g085720	32.020	406	242	10	20	416	9	389	1.59e-49	174
MS.gene72611.t1	MTR_1g056840	29.698	431	224	12	20	415	2	388	6.97e-47	166
MS.gene72611.t1	MTR_1g056910	30.348	402	253	11	22	414	3	386	7.86e-43	155
MS.gene72611.t1	MTR_8g092870	26.415	424	281	9	18	429	3	407	3.68e-41	151
MS.gene72611.t1	MTR_5g015210	29.219	397	242	9	25	415	67	430	9.51e-38	143
MS.gene72611.t1	MTR_3g095730	27.230	426	263	15	23	429	11	408	1.47e-36	139
MS.gene72611.t1	MTR_4g051515	27.670	412	258	15	22	415	3	392	4.68e-34	132
MS.gene72611.t1	MTR_4g485520	27.119	413	250	14	18	414	99	476	4.74e-29	119
MS.gene72611.t1	MTR_4g485520	27.119	413	250	14	18	414	267	644	9.97e-29	119
MS.gene72611.t1	MTR_4g063800	28.230	418	242	16	24	422	254	632	1.29e-27	116
MS.gene72611.t1	MTR_1g017770	26.000	400	256	9	25	415	45	413	2.33e-27	113
MS.gene72611.t1	MTR_3g096370	27.670	412	264	14	18	416	3	393	4.06e-26	109
MS.gene72611.t1	MTR_5g083030	25.926	432	249	10	21	417	289	684	8.35e-26	110
MS.gene72611.t1	MTR_7g078330	26.382	398	260	12	23	416	272	640	8.38e-25	107
MS.gene72611.t1	MTR_7g059405	26.341	410	247	17	24	417	263	633	1.31e-23	103
MS.gene72611.t1	MTR_1g094215	27.857	420	245	15	24	425	63	442	7.44e-23	100
MS.gene72611.t1	MTR_4g028960	51.948	77	37	0	20	96	275	351	1.27e-22	101
MS.gene72611.t1	MTR_1g094215	27.857	420	245	15	24	425	246	625	4.36e-22	99.4
MS.gene72611.t1	MTR_3g085610	41.346	104	53	2	11	114	268	363	3.53e-21	97.1
MS.gene72611.t1	MTR_7g053260	36.029	136	71	3	7	141	59	179	8.42e-21	94.7
MS.gene72611.t1	MTR_2g018010	52.857	70	33	0	23	92	290	359	1.31e-20	95.1
MS.gene72611.t1	MTR_3g078160	42.529	87	50	0	10	96	260	346	2.69e-20	94.0
MS.gene72611.t1	MTR_3g078160	42.529	87	50	0	10	96	260	346	3.11e-20	94.0
MS.gene72611.t1	MTR_3g078160	42.529	87	50	0	10	96	260	346	3.57e-20	93.2
MS.gene72611.t1	MTR_4g107010	52.857	70	33	0	26	95	281	350	4.61e-20	93.6
MS.gene72611.t1	MTR_8g080280	27.160	243	144	7	10	232	267	496	6.17e-19	90.1
MS.gene72611.t1	MTR_7g116600	50.617	81	36	2	26	106	79	155	1.04e-18	88.6
MS.gene72611.t1	MTR_5g034440	53.030	66	31	0	27	92	282	347	3.27e-18	87.8
MS.gene72611.t1	MTR_4g091880	47.222	72	37	1	21	91	1	72	2.91e-17	83.2
MS.gene72611.t1	MTR_4g091880	47.222	72	37	1	21	91	1	72	3.20e-17	83.2
MS.gene72611.t1	MTR_1g069845	44.286	70	39	0	23	92	122	191	7.26e-17	83.2
MS.gene72611.t1	MTR_7g097020	46.269	67	36	0	29	95	245	311	8.30e-17	83.6
MS.gene72611.t1	MTR_1g069845	44.286	70	39	0	23	92	243	312	1.21e-16	82.8
MS.gene72611.t1	MTR_1g069845	44.286	70	39	0	23	92	239	308	1.28e-16	82.8
MS.gene72611.t1	MTR_1g079450	39.394	99	53	3	20	118	58	149	5.93e-16	80.1
MS.gene72611.t1	MTR_8g077205	41.860	86	46	2	21	106	159	240	1.73e-15	79.3
MS.gene72611.t1	MTR_8g077205	41.860	86	46	2	21	106	271	352	1.97e-15	79.0
MS.gene72611.t1	MTR_3g466220	38.272	81	50	0	15	95	223	303	4.86e-15	77.8
MS.gene72611.t1	MTR_3g466220	38.272	81	50	0	15	95	202	282	4.89e-15	77.8
MS.gene72611.t1	MTR_5g020570	39.286	84	47	2	23	106	271	350	4.23e-14	75.1
MS.gene72611.t1	MTR_5g020570	39.286	84	47	2	23	106	286	365	5.06e-14	74.7
MS.gene72611.t1	MTR_2g011140	43.750	64	36	0	28	91	18	81	6.58e-14	73.2
MS.gene72611.t1	MTR_7g117890	30.337	178	100	6	11	187	244	398	6.59e-14	74.7
MS.gene72611.t1	MTR_8g103227	46.269	67	35	1	26	91	396	462	5.79e-13	71.6
MS.gene72611.t1	MTR_3g096370	62.791	43	16	0	18	60	3	45	1.06e-12	68.9
MS.gene72611.t1	MTR_2g096850	40.000	70	42	0	22	91	586	655	1.35e-12	70.1
MS.gene72611.t1	MTR_2g096850	40.000	70	42	0	22	91	737	806	1.46e-12	70.1
MS.gene72611.t1	MTR_3g115670	42.105	76	39	1	25	95	30	105	1.79e-12	70.1
MS.gene72611.t1	MTR_1g093965	43.750	64	33	1	38	101	1	61	2.62e-12	65.9
MS.gene72611.t1	MTR_1g090320	41.463	82	39	3	16	95	510	584	3.11e-12	69.3
MS.gene72611.t1	MTR_1g090320	41.463	82	39	3	16	95	512	586	3.28e-12	69.3
MS.gene72611.t1	MTR_7g077780	39.394	66	40	0	26	91	827	892	3.87e-12	68.9
MS.gene72611.t1	MTR_1g093995	43.750	64	33	1	38	101	1	61	5.77e-12	65.1
MS.gene72611.t1	MTR_3g008270	45.455	66	35	1	26	91	731	795	6.29e-12	68.2
MS.gene72611.t1	MTR_5g032010	37.313	67	42	0	25	91	732	798	1.11e-11	67.4
MS.gene72611.t1	MTR_1g100820	37.879	66	41	0	26	91	647	712	1.75e-11	66.6
MS.gene72611.t1	MTR_1g100820	37.879	66	41	0	26	91	649	714	1.75e-11	66.6
MS.gene72611.t1	MTR_7g005940	39.726	73	41	2	25	95	595	666	7.07e-11	65.1
MS.gene72611.t1	MTR_7g005940	39.726	73	41	2	25	95	595	666	7.59e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72611.t1	AT5G37490	43.891	442	229	6	5	438	5	435	1.52e-113	340
MS.gene72611.t1	AT1G66160	41.745	424	220	9	21	438	29	431	2.02e-108	327
MS.gene72611.t1	AT1G66160	39.387	424	214	10	21	438	29	415	4.56e-96	295
MS.gene72611.t1	AT3G02840	39.595	346	196	8	100	438	40	379	8.84e-73	234
MS.gene72611.t1	AT2G35930	33.172	413	238	12	16	415	3	390	1.88e-57	194
MS.gene72611.t1	AT1G49780	32.029	409	247	8	20	416	9	398	3.55e-48	170
MS.gene72611.t1	AT3G19380	31.392	395	240	10	20	403	9	383	9.60e-47	166
MS.gene72611.t1	AT3G11840	29.358	436	249	13	22	414	7	426	1.39e-41	153
MS.gene72611.t1	AT3G52450	45.652	138	67	4	20	155	2	133	3.71e-34	132
MS.gene72611.t1	AT5G09800	27.295	403	257	13	25	416	11	388	4.17e-32	126
MS.gene72611.t1	AT5G64660	27.603	413	269	12	18	416	3	399	6.65e-31	123
MS.gene72611.t1	AT3G49810	27.318	399	247	11	25	415	64	427	9.61e-31	123
MS.gene72611.t1	AT5G65920	26.847	406	254	11	25	422	60	430	6.56e-29	118
MS.gene72611.t1	AT5G65920	26.847	406	254	11	25	422	60	430	6.56e-29	118
MS.gene72611.t1	AT3G18710	25.301	415	260	15	23	416	10	395	3.04e-28	115
MS.gene72611.t1	AT3G54850	27.561	410	246	14	24	417	247	621	1.25e-25	110
MS.gene72611.t1	AT2G28830	27.160	405	252	14	23	413	182	557	2.73e-25	108
MS.gene72611.t1	AT1G71020	26.265	415	270	13	12	416	82	470	9.12e-25	106
MS.gene72611.t1	AT1G23030	27.644	416	254	16	12	416	228	607	1.15e-24	107
MS.gene72611.t1	AT1G71020	26.265	415	270	13	12	416	230	618	1.54e-24	107
MS.gene72611.t1	AT3G46510	24.941	425	272	16	3	414	235	625	8.58e-22	98.6
MS.gene72611.t1	AT5G42340	26.587	252	149	5	14	232	279	527	8.29e-21	95.5
MS.gene72611.t1	AT5G01830	51.389	72	35	0	25	96	274	345	3.00e-20	94.0
MS.gene72611.t1	AT1G29340	51.429	70	34	0	23	92	303	372	2.75e-19	91.3
MS.gene72611.t1	AT1G24330	33.533	167	90	6	2	153	246	406	1.08e-18	89.4
MS.gene72611.t1	AT1G24330	33.533	167	90	6	2	153	246	406	1.08e-18	89.4
MS.gene72611.t1	AT1G27910	38.596	114	62	3	14	125	268	375	1.24e-18	89.0
MS.gene72611.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.02e-18	88.2
MS.gene72611.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.24e-18	88.2
MS.gene72611.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.24e-18	88.2
MS.gene72611.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.24e-18	88.2
MS.gene72611.t1	AT3G07360	45.570	79	40	1	23	101	72	147	1.11e-17	85.5
MS.gene72611.t1	AT1G10560	32.414	145	84	2	31	164	294	435	2.10e-17	85.1
MS.gene72611.t1	AT3G54790	43.421	76	43	0	23	98	200	275	8.69e-17	83.2
MS.gene72611.t1	AT1G01680	37.931	87	49	1	9	95	222	303	1.16e-16	80.9
MS.gene72611.t1	AT3G54790	43.421	76	43	0	23	98	236	311	1.20e-16	82.8
MS.gene72611.t1	AT3G54790	43.421	76	43	0	23	98	236	311	1.20e-16	82.8
MS.gene72611.t1	AT4G21350	47.222	72	37	1	21	91	1	72	3.44e-16	80.1
MS.gene72611.t1	AT1G60190	32.215	149	83	4	27	164	280	421	3.02e-15	78.6
MS.gene72611.t1	AT4G36550	37.500	72	45	0	24	95	218	289	1.87e-14	76.3
MS.gene72611.t1	AT3G61390	43.939	66	37	0	26	91	279	344	3.53e-14	73.9
MS.gene72611.t1	AT3G61390	43.939	66	37	0	26	91	354	419	4.94e-14	73.9
MS.gene72611.t1	AT2G23140	36.082	97	58	1	23	119	231	323	1.38e-13	73.6
MS.gene72611.t1	AT2G23140	36.082	97	58	1	23	119	228	320	1.40e-13	73.6
MS.gene72611.t1	AT2G23140	36.082	97	58	1	23	119	228	320	1.40e-13	73.6
MS.gene72611.t1	AT3G49060	42.424	66	38	0	26	91	736	801	1.61e-13	73.2
MS.gene72611.t1	AT3G49060	42.424	66	38	0	26	91	726	791	1.61e-13	73.2
MS.gene72611.t1	AT3G49060	42.424	66	38	0	26	91	703	768	1.65e-13	73.2
MS.gene72611.t1	AT2G23140	36.082	97	58	1	23	119	39	131	1.78e-13	72.8
MS.gene72611.t1	AT2G23140	36.082	97	58	1	23	119	39	131	1.78e-13	72.8
MS.gene72611.t1	AT1G01660	38.571	70	43	0	26	95	499	568	1.80e-13	72.8
MS.gene72611.t1	AT1G01660	38.571	70	43	0	26	95	498	567	1.86e-13	72.8
MS.gene72611.t1	AT2G45920	42.424	66	38	0	26	91	326	391	2.18e-13	72.0
MS.gene72611.t1	AT1G56040	38.824	85	51	1	7	90	334	418	1.32e-12	69.7
MS.gene72611.t1	AT5G67340	36.986	73	46	0	23	95	238	310	1.50e-12	70.1
MS.gene72611.t1	AT5G67340	36.986	73	46	0	23	95	238	310	1.50e-12	70.1
MS.gene72611.t1	AT1G56030	43.077	65	37	0	26	90	324	388	1.94e-12	68.9
MS.gene72611.t1	AT5G51270	40.845	71	41	1	26	96	736	805	2.35e-12	69.7
MS.gene72611.t1	AT5G51270	40.845	71	41	1	26	96	736	805	2.35e-12	69.7
MS.gene72611.t1	AT5G65500	41.333	75	41	1	17	91	718	789	2.40e-12	69.7
MS.gene72611.t1	AT5G65500	41.333	75	41	1	17	91	710	781	2.43e-12	69.7
MS.gene72611.t1	AT1G01670	40.909	66	39	0	26	91	211	276	1.11e-11	65.5
MS.gene72611.t1	AT1G01670	40.909	66	39	0	26	91	295	360	1.99e-11	65.9
MS.gene72611.t1	AT1G01670	40.909	66	39	0	26	91	295	360	1.99e-11	65.9
MS.gene72611.t1	AT5G18340	32.110	109	67	3	12	120	61	162	4.79e-11	65.1
MS.gene72611.t1	AT5G18320	30.709	127	82	4	23	146	70	193	6.33e-11	64.7

Find 53 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGAAGGCTTTAACTATTT+TGG	0.085501	6.4:-48797400	MS.gene72611:CDS
TCAAATTGATGAATCCTTAT+AGG	0.188148	6.4:-48797110	MS.gene72611:CDS
AATCATCATTGAGGATAATT+TGG	0.200592	6.4:-48797536	MS.gene72611:CDS
TTCATCAAATCAAGAGTAAT+TGG	0.206017	6.4:+48798254	None:intergenic
AGTATTCAGTTTCTACTATA+TGG	0.256281	6.4:-48797272	MS.gene72611:CDS
CTTCTTTACATTGCATGATT+TGG	0.259797	6.4:-48797725	MS.gene72611:CDS
CAAATTGATGAATCCTTATA+GGG	0.287604	6.4:-48797109	MS.gene72611:CDS
TGATTCCAAATCATTCTCTT+AGG	0.293043	6.4:-48798115	MS.gene72611:CDS
TTGAAAGAGATTCTTTCTTT+TGG	0.293237	6.4:-48797654	MS.gene72611:CDS
TGACTTAACAATTCCTTTGT+TGG	0.326123	6.4:-48797331	MS.gene72611:CDS
ATCGAGCTAACCGGTATTCT+TGG	0.327497	6.4:+48798032	None:intergenic
AAGTTCAGTTGCTTTATCTT+TGG	0.334075	6.4:+48797134	None:intergenic
TCTTCCAATTCAGCCCTATA+AGG	0.335630	6.4:+48797096	None:intergenic
ATTGGACACCTAAATTGATT+TGG	0.335859	6.4:+48798272	None:intergenic
GGTATCACTACCTCCATGTT+TGG	0.338704	6.4:+48798293	None:intergenic
ATATGGAAAATGTGCAAATA+TGG	0.343111	6.4:-48797255	MS.gene72611:CDS
GTTTAAGATGGTGTTAGGTT+GGG	0.345500	6.4:-48798354	None:intergenic
TAGTGATACCAAATCAATTT+AGG	0.346434	6.4:-48798280	MS.gene72611:CDS
GAATCCTTATAGGGCTGAAT+TGG	0.347326	6.4:-48797100	MS.gene72611:CDS
TTTGAAGCATCAAGATGTTA+AGG	0.349669	6.4:-48797700	MS.gene72611:CDS
TGTTTAAGATGGTGTTAGGT+TGG	0.352531	6.4:-48798355	None:intergenic
AAAGTGTTTAAGATGGTGTT+AGG	0.352803	6.4:-48798359	None:intergenic
GCAAAAGGTTTGGATCAATA+TGG	0.357148	6.4:-48797972	MS.gene72611:CDS
TTCATTTGTTGAATTGGGTT+TGG	0.366135	6.4:-48797469	MS.gene72611:CDS
GAAACATGTAGAATCACTTA+TGG	0.368385	6.4:-48797625	MS.gene72611:CDS
TTCAATACTCTCTCTATCAT+AGG	0.380174	6.4:+48798202	None:intergenic
TGGTGTTAGGTTGGGGAAGA+AGG	0.382817	6.4:-48798346	MS.gene72611:CDS
ATTATCCTAAGAGAATGATT+TGG	0.383748	6.4:+48798110	None:intergenic
TCAATATGGTTGTATTGAAT+TGG	0.405007	6.4:-48797958	MS.gene72611:CDS
TAAGGAATCAGCAAAAGGTT+TGG	0.411626	6.4:-48797982	MS.gene72611:CDS
TTGAAGCATCAAGATGTTAA+GGG	0.415181	6.4:-48797699	MS.gene72611:CDS
TTAGGATAATGATTCAAGAT+TGG	0.432186	6.4:-48798097	MS.gene72611:CDS
GAAACATCGATCGAGCTAAC+CGG	0.450457	6.4:+48798023	None:intergenic
CGAGTTAAGGAATCAGCAAA+AGG	0.455195	6.4:-48797987	MS.gene72611:CDS
GATCCTGTGACATTGTCAAC+AGG	0.477744	6.4:-48798230	MS.gene72611:CDS
TTTAAGATGGTGTTAGGTTG+GGG	0.480803	6.4:-48798353	None:intergenic
TGTGTTGAGAATAGACAAAA+TGG	0.498744	6.4:-48798074	MS.gene72611:CDS
CTATTATTGGTATTGCAAGT+TGG	0.507419	6.4:-48797171	MS.gene72611:CDS
ATTGAAAGATGGTTCAATGA+AGG	0.518770	6.4:-48798185	MS.gene72611:CDS
ATAGAGAGAGTATTGAAAGA+TGG	0.521682	6.4:-48798196	MS.gene72611:CDS
ATTCCTGTTGACAATGTCAC+AGG	0.541677	6.4:+48798227	None:intergenic
GAATCACTTATGGAAATTGA+AGG	0.552079	6.4:-48797615	MS.gene72611:CDS
CAAAGGTGGAAAACCAAACA+TGG	0.558197	6.4:-48798306	MS.gene72611:CDS
TTGATTGAAGCACTTCAAGT+TGG	0.561935	6.4:-48797207	MS.gene72611:CDS
GATTCCTACTCCAAGAATAC+CGG	0.564162	6.4:-48798042	MS.gene72611:CDS
ACTGAATACTCTGTGGTCAA+AGG	0.567201	6.4:+48797285	None:intergenic
CTTACAACTTGATTTGTGAG+AGG	0.581086	6.4:+48798149	None:intergenic
CTAACCGGTATTCTTGGAGT+AGG	0.590630	6.4:+48798038	None:intergenic
AATGGAGTAATGAAAGTGAG+AGG	0.609225	6.4:-48797920	MS.gene72611:CDS
ATAGACAAAATGGTGTTGAG+AGG	0.615873	6.4:-48798064	MS.gene72611:CDS
CAATCACAAAATCATCATTG+AGG	0.618015	6.4:-48797545	MS.gene72611:CDS
AGTAGAAACTGAATACTCTG+TGG	0.711233	6.4:+48797278	None:intergenic
AGGTGGAAAACCAAACATGG+AGG	0.758131	6.4:-48798303	MS.gene72611:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTCATTTGTTGAATT+GGG	-	chr6.4:48797926-48797945	MS.gene72611:CDS	15.0%
!!!	GAAATTTTCATTTGTTGAAT+TGG	-	chr6.4:48797925-48797944	MS.gene72611:CDS	20.0%
!!!	TATTGAATTGGTTCAAAAAA+TGG	-	chr6.4:48797454-48797473	MS.gene72611:CDS	20.0%
!	AAAAAATGCATAGTTGAGAA+TGG	-	chr6.4:48797506-48797525	MS.gene72611:CDS	25.0%
!	AAGAACAAGAAAAACAACAA+AGG	-	chr6.4:48797077-48797096	MS.gene72611:CDS	25.0%
!	AATCATCATTGAGGATAATT+TGG	-	chr6.4:48797864-48797883	MS.gene72611:CDS	25.0%
!	AGTATTCAGTTTCTACTATA+TGG	-	chr6.4:48798128-48798147	MS.gene72611:CDS	25.0%
!	ATATGGAAAATGTGCAAATA+TGG	-	chr6.4:48798145-48798164	MS.gene72611:CDS	25.0%
!	ATTATCCTAAGAGAATGATT+TGG	+	chr6.4:48797293-48797312	None:intergenic	25.0%
!	CAAATTGATGAATCCTTATA+GGG	-	chr6.4:48798291-48798310	MS.gene72611:CDS	25.0%
!	TAGTGATACCAAATCAATTT+AGG	-	chr6.4:48797120-48797139	MS.gene72611:CDS	25.0%
!	TATGGTCACAAAAATTATGA+AGG	-	chr6.4:48798163-48798182	MS.gene72611:CDS	25.0%
!	TCAAATTGATGAATCCTTAT+AGG	-	chr6.4:48798290-48798309	MS.gene72611:CDS	25.0%
!	TCAATATGGTTGTATTGAAT+TGG	-	chr6.4:48797442-48797461	MS.gene72611:CDS	25.0%
!	TTAGAAGCTCAAAAATCTTT+AGG	-	chr6.4:48797644-48797663	MS.gene72611:CDS	25.0%
!	TTAGGATAATGATTCAAGAT+TGG	-	chr6.4:48797303-48797322	MS.gene72611:CDS	25.0%
!	TTCATCAAATCAAGAGTAAT+TGG	+	chr6.4:48797149-48797168	None:intergenic	25.0%
!!	AATGATGATTTTGTGATTGT+TGG	+	chr6.4:48797854-48797873	None:intergenic	25.0%
!!	TGATTTTATCTGCACTTAAT+TGG	-	chr6.4:48797606-48797625	MS.gene72611:CDS	25.0%
!!	TTGAAAGAGATTCTTTCTTT+TGG	-	chr6.4:48797746-48797765	MS.gene72611:CDS	25.0%
!!!	AAGATTTTTTTGACCAACAA+AGG	+	chr6.4:48798085-48798104	None:intergenic	25.0%
	AACAAGAAAAACAACAAAGG+TGG	-	chr6.4:48797080-48797099	MS.gene72611:CDS	30.0%
	ATTGAAAGATGGTTCAATGA+AGG	-	chr6.4:48797215-48797234	MS.gene72611:CDS	30.0%
	ATTGGACACCTAAATTGATT+TGG	+	chr6.4:48797131-48797150	None:intergenic	30.0%
	CAATCACAAAATCATCATTG+AGG	-	chr6.4:48797855-48797874	MS.gene72611:CDS	30.0%
	CTATTATTGGTATTGCAAGT+TGG	-	chr6.4:48798229-48798248	MS.gene72611:CDS	30.0%
	CTTCTTTACATTGCATGATT+TGG	-	chr6.4:48797675-48797694	MS.gene72611:CDS	30.0%
	GAAACATGTAGAATCACTTA+TGG	-	chr6.4:48797775-48797794	MS.gene72611:CDS	30.0%
	GAATCACTTATGGAAATTGA+AGG	-	chr6.4:48797785-48797804	MS.gene72611:CDS	30.0%
	TGACTTAACAATTCCTTTGT+TGG	-	chr6.4:48798069-48798088	MS.gene72611:CDS	30.0%
	TGATTCCAAATCATTCTCTT+AGG	-	chr6.4:48797285-48797304	MS.gene72611:CDS	30.0%
	TGGTTCAAAAAATGGAGAAA+TGG	-	chr6.4:48797462-48797481	MS.gene72611:CDS	30.0%
	TGTGTTGAGAATAGACAAAA+TGG	-	chr6.4:48797326-48797345	MS.gene72611:CDS	30.0%
	TTCAATACTCTCTCTATCAT+AGG	+	chr6.4:48797201-48797220	None:intergenic	30.0%
!	AAGTTCAGTTGCTTTATCTT+TGG	+	chr6.4:48798269-48798288	None:intergenic	30.0%
!	TGCTTTTCAGAAGCTATTAT+TGG	-	chr6.4:48798216-48798235	MS.gene72611:CDS	30.0%
!	TTCATTTGTTGAATTGGGTT+TGG	-	chr6.4:48797931-48797950	MS.gene72611:CDS	30.0%
!	TTGAAGCATCAAGATGTTAA+GGG	-	chr6.4:48797701-48797720	MS.gene72611:CDS	30.0%
!	TTTGAAGCATCAAGATGTTA+AGG	-	chr6.4:48797700-48797719	MS.gene72611:CDS	30.0%
!	TTTTGAGCTTCTAATTGAAG+TGG	+	chr6.4:48797638-48797657	None:intergenic	30.0%
!!	ATAGAGAGAGTATTGAAAGA+TGG	-	chr6.4:48797204-48797223	MS.gene72611:CDS	30.0%
!!	TGAGAAGGCTTTAACTATTT+TGG	-	chr6.4:48798000-48798019	MS.gene72611:CDS	30.0%
	AATGGAGTAATGAAAGTGAG+AGG	-	chr6.4:48797480-48797499	MS.gene72611:CDS	35.0%
	AGTAGAAACTGAATACTCTG+TGG	+	chr6.4:48798125-48798144	None:intergenic	35.0%
	CTTAACTCGAAAAAGAAGCT+CGG	+	chr6.4:48797402-48797421	None:intergenic	35.0%
	GCAAAAGGTTTGGATCAATA+TGG	-	chr6.4:48797428-48797447	MS.gene72611:CDS	35.0%
	TAAGGAATCAGCAAAAGGTT+TGG	-	chr6.4:48797418-48797437	MS.gene72611:CDS	35.0%
!	ATAGACAAAATGGTGTTGAG+AGG	-	chr6.4:48797336-48797355	MS.gene72611:CDS	35.0%
!	TTGATTGAAGCACTTCAAGT+TGG	-	chr6.4:48798193-48798212	MS.gene72611:CDS	35.0%
!!	AGAAAAAAGCACTTGTGAGA+AGG	-	chr6.4:48797985-48798004	MS.gene72611:CDS	35.0%
!!	CTTACAACTTGATTTGTGAG+AGG	+	chr6.4:48797254-48797273	None:intergenic	35.0%
	ACTGAATACTCTGTGGTCAA+AGG	+	chr6.4:48798118-48798137	None:intergenic	40.0%
	ATTCCTGTTGACAATGTCAC+AGG	+	chr6.4:48797176-48797195	None:intergenic	40.0%
	CAAAGGTGGAAAACCAAACA+TGG	-	chr6.4:48797094-48797113	MS.gene72611:CDS	40.0%
	CGAGTTAAGGAATCAGCAAA+AGG	-	chr6.4:48797413-48797432	MS.gene72611:CDS	40.0%
	GAATCCTTATAGGGCTGAAT+TGG	-	chr6.4:48798300-48798319	MS.gene72611:CDS	40.0%
	GATTCCTACTCCAAGAATAC+CGG	-	chr6.4:48797358-48797377	MS.gene72611:CDS	40.0%
	TCTTCCAATTCAGCCCTATA+AGG	+	chr6.4:48798307-48798326	None:intergenic	40.0%
!!!	CGAGCTTCTTTTTCGAGTTA+AGG	-	chr6.4:48797400-48797419	MS.gene72611:CDS	40.0%
	AGGTGGAAAACCAAACATGG+AGG	-	chr6.4:48797097-48797116	MS.gene72611:CDS	45.0%
	ATCGAGCTAACCGGTATTCT+TGG	+	chr6.4:48797371-48797390	None:intergenic	45.0%
	CTAACCGGTATTCTTGGAGT+AGG	+	chr6.4:48797365-48797384	None:intergenic	45.0%
	GAAACATCGATCGAGCTAAC+CGG	+	chr6.4:48797380-48797399	None:intergenic	45.0%
	GATCCTGTGACATTGTCAAC+AGG	-	chr6.4:48797170-48797189	MS.gene72611:CDS	45.0%
	GGTATCACTACCTCCATGTT+TGG	+	chr6.4:48797110-48797129	None:intergenic	45.0%
!!	TGGTGTTAGGTTGGGGAAGA+AGG	-	chr6.4:48797054-48797073	MS.gene72611:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	48797053	48798369	48797053	ID=MS.gene72611
chr6.4	mRNA	48797053	48798369	48797053	ID=MS.gene72611.t1;Parent=MS.gene72611
chr6.4	exon	48797053	48798369	48797053	ID=MS.gene72611.t1.exon1;Parent=MS.gene72611.t1
chr6.4	CDS	48797053	48798369	48797053	ID=cds.MS.gene72611.t1;Parent=MS.gene72611.t1

Gene Sequence

>MS.gene72611

ATGGTGTTAGGTTGGGGAAGAAGGAAGAACAAGAAAAACAACAAAGGTGGAAAACCAAACATGGAGGTAGTGATACCAAATCAATTTAGGTGTCCAATTACTCTTGATTTGATGAAAGATCCTGTGACATTGTCAACAGGAATAACCTATGATAGAGAGAGTATTGAAAGATGGTTCAATGAAGGAAACTATACATGTCCTCTCACAAATCAAGTTGTAAGAAACTTTGATATGATTCCAAATCATTCTCTTAGGATAATGATTCAAGATTGGTGTGTTGAGAATAGACAAAATGGTGTTGAGAGGATTCCTACTCCAAGAATACCGGTTAGCTCGATCGATGTTTCCGAGCTTCTTTTTCGAGTTAAGGAATCAGCAAAAGGTTTGGATCAATATGGTTGTATTGAATTGGTTCAAAAAATGGAGAAATGGAGTAATGAAAGTGAGAGGAACAAAAAATGCATAGTTGAGAATGGAGCAACTAGTGCATTAGCTTTAGCATTTGATGCTTTTGCTAATGATTCTATTGAGAAAAATGTTCTTGTTCTTGAAGTGATTTTATCTGCACTTAATTGGATGTTTCCACTTCAATTAGAAGCTCAAAAATCTTTAGGTTCAAAAGCTTCTTTACATTGCATGATTTGGTTTTTGAAGCATCAAGATGTTAAGGGAAAAGAAAAAGCTATTATTGCATTGAAAGAGATTCTTTCTTTTGGTGATGAGAAACATGTAGAATCACTTATGGAAATTGAAGGAGTTAATGAGCTTTTAATTGAGTTCATTAACAAGAGAATTAGTCCAACAATCACAAAATCATCATTGAGGATAATTTGGTACTTAGTTTCATCAAATTCTAATTTCAATGAAAAAATGAAATTTTCATTTGTTGAATTGGGTTTGGTATCTTCAATTTTGCATATTCTTATTGACTCAGAAAAAAGCACTTGTGAGAAGGCTTTAACTATTTTGGATTCTTTATTAAGCTGTGACTTTGCAAGAGAAACAGCTTATGAAAATGACTTAACAATTCCTTTGTTGGTCAAAAAAATCTTGAGAGTTTCACCTTTGACCACAGAGTATTCAGTTTCTACTATATGGAAAATGTGCAAATATGGTCACAAAAATTATGAAGGAAAAGCTTTGATTGAAGCACTTCAAGTTGGTGCTTTTCAGAAGCTATTATTGGTATTGCAAGTTGGATGTAATGATGAGACCAAAGATAAAGCAACTGAACTTCTCAAATTGATGAATCCTTATAGGGCTGAATTGGAAGATTGTGTTGATTCAGATTTCAAGAATCTTAAGAGATCATATTAA

Protein sequence

>MS.gene72611.t1

MVLGWGRRKNKKNNKGGKPNMEVVIPNQFRCPITLDLMKDPVTLSTGITYDRESIERWFNEGNYTCPLTNQVVRNFDMIPNHSLRIMIQDWCVENRQNGVERIPTPRIPVSSIDVSELLFRVKESAKGLDQYGCIELVQKMEKWSNESERNKKCIVENGATSALALAFDAFANDSIEKNVLVLEVILSALNWMFPLQLEAQKSLGSKASLHCMIWFLKHQDVKGKEKAIIALKEILSFGDEKHVESLMEIEGVNELLIEFINKRISPTITKSSLRIIWYLVSSNSNFNEKMKFSFVELGLVSSILHILIDSEKSTCEKALTILDSLLSCDFARETAYENDLTIPLLVKKILRVSPLTTEYSVSTIWKMCKYGHKNYEGKALIEALQVGAFQKLLLVLQVGCNDETKDKATELLKLMNPYRAELEDCVDSDFKNLKRSY