AlfalfaGEDB | MS.gene061546

M. sativa cultivar XinJiangDaYe / MS.gene061546

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061546.t1	XP_003600684.1	96.8	439	14	0	1	439	1	439	3.80E-242	847

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061546.t1	Q5PNY6	45.9	414	207	8	33	438	28	432	2.5e-93	344.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061546.t1	G7J8Q8	96.8	439	14	0	1	439	1	439	2.7e-242	847.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04998	MS.gene061546	0.931666	2.70E-94	-1.69E-46
MS.gene051226	MS.gene061546	0.82815	1.05E-54	-1.69E-46
MS.gene051837	MS.gene061546	0.836798	7.57E-57	-1.69E-46
MS.gene057093	MS.gene061546	0.847422	1.17E-59	-1.69E-46
MS.gene057432	MS.gene061546	0.800313	1.53E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene061546	MS.gene45454	PPI
MS.gene45456	MS.gene061546	PPI
MS.gene061546	MS.gene064492	PPI
MS.gene04998	MS.gene061546	PPI
MS.gene061546	MS.gene26139	PPI
MS.gene061546	MS.gene72611	PPI
MS.gene061546	MS.gene048677	PPI
MS.gene025667	MS.gene061546	PPI
MS.gene061546	MS.gene066130	PPI
MS.gene45455	MS.gene061546	PPI
MS.gene061546	MS.gene26489	PPI
MS.gene061546	MS.gene022190	PPI
MS.gene064492	MS.gene061546	PPI
MS.gene042912	MS.gene061546	PPI
MS.gene93972	MS.gene061546	PPI
MS.gene034751	MS.gene061546	PPI
MS.gene034753	MS.gene061546	PPI
MS.gene061546	MS.gene066129	PPI
MS.gene017373	MS.gene061546	PPI
MS.gene061546	MS.gene41033	PPI
MS.gene048677	MS.gene061546	PPI
MS.gene061546	MS.gene93972	PPI
MS.gene008306	MS.gene061546	PPI
MS.gene007121	MS.gene061546	PPI
MS.gene41032	MS.gene061546	PPI
MS.gene061543	MS.gene061546	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061546.t1	MTR_3g065080	96.811	439	14	0	1	439	1	439	0.0	861
MS.gene061546.t1	MTR_5g077510	64.334	443	149	8	1	438	1	439	0.0	545
MS.gene061546.t1	MTR_6g071340	47.733	419	201	7	33	438	2	415	2.56e-125	370
MS.gene061546.t1	MTR_5g048050	47.494	419	202	7	33	438	22	435	2.10e-123	365
MS.gene061546.t1	MTR_7g106340	35.225	423	247	11	32	436	4	417	2.09e-61	205
MS.gene061546.t1	MTR_2g087350	35.238	420	240	11	31	436	1	402	2.83e-60	202
MS.gene061546.t1	MTR_4g085720	32.767	412	247	10	34	434	12	404	1.11e-58	198
MS.gene061546.t1	MTR_1g056880	33.937	442	244	11	32	436	3	433	1.49e-58	198
MS.gene061546.t1	MTR_1g056870	33.484	442	246	11	32	436	3	433	5.32e-58	197
MS.gene061546.t1	MTR_1g056840	32.426	441	226	13	32	436	3	407	1.08e-50	177
MS.gene061546.t1	MTR_1g056910	31.830	399	246	13	31	416	1	386	1.57e-48	171
MS.gene061546.t1	MTR_4g051515	27.990	418	264	14	33	429	3	404	6.58e-41	150
MS.gene061546.t1	MTR_4g063800	26.379	417	266	13	25	426	244	634	1.38e-35	139
MS.gene061546.t1	MTR_5g083030	27.711	415	250	13	34	419	291	684	8.23e-34	134
MS.gene061546.t1	MTR_4g485520	25.908	413	265	12	24	418	89	478	1.91e-33	131
MS.gene061546.t1	MTR_3g095730	24.821	419	281	11	34	435	11	412	4.14e-33	129
MS.gene061546.t1	MTR_8g092870	26.014	419	278	12	34	435	8	411	4.98e-33	129
MS.gene061546.t1	MTR_4g485520	25.935	401	260	11	32	418	269	646	6.16e-33	131
MS.gene061546.t1	MTR_7g059405	26.799	403	243	17	36	419	264	633	6.95e-32	128
MS.gene061546.t1	MTR_5g015210	27.390	387	253	8	36	417	67	430	3.84e-29	119
MS.gene061546.t1	MTR_4g107010	29.365	378	227	13	3	360	247	604	2.05e-28	118
MS.gene061546.t1	MTR_3g096370	68.571	70	22	0	33	102	7	76	8.54e-27	111
MS.gene061546.t1	MTR_7g078330	24.085	328	220	8	9	324	240	550	1.57e-26	112
MS.gene061546.t1	MTR_1g017770	27.885	416	243	13	26	417	31	413	7.88e-26	109
MS.gene061546.t1	MTR_2g018010	28.125	320	180	14	34	328	290	584	9.89e-26	110
MS.gene061546.t1	MTR_4g028960	28.435	313	164	13	22	319	262	529	1.97e-25	109
MS.gene061546.t1	MTR_4g091880	24.359	390	238	13	35	420	4	340	7.85e-24	102
MS.gene061546.t1	MTR_1g094215	26.012	346	226	9	36	370	64	390	1.46e-23	102
MS.gene061546.t1	MTR_1g094215	26.361	349	221	11	36	370	247	573	1.69e-23	103
MS.gene061546.t1	MTR_3g085610	28.571	308	168	8	37	335	281	545	3.30e-23	103
MS.gene061546.t1	MTR_4g091880	24.685	397	256	13	35	420	4	368	3.53e-23	100
MS.gene061546.t1	MTR_3g078160	27.385	325	195	12	37	341	276	579	1.96e-21	97.4
MS.gene061546.t1	MTR_3g078160	27.385	325	195	12	37	341	276	579	2.32e-21	97.4
MS.gene061546.t1	MTR_7g116600	47.000	100	45	3	32	131	74	165	1.88e-20	93.6
MS.gene061546.t1	MTR_7g053260	52.174	69	33	0	34	102	76	144	5.96e-20	92.4
MS.gene061546.t1	MTR_3g078160	28.417	278	163	10	37	295	276	536	1.59e-19	91.3
MS.gene061546.t1	MTR_5g034440	45.133	113	46	4	40	141	284	391	2.30e-19	91.3
MS.gene061546.t1	MTR_1g069845	47.143	70	37	0	34	103	122	191	1.57e-18	88.6
MS.gene061546.t1	MTR_1g069845	47.143	70	37	0	34	103	243	312	2.47e-18	88.2
MS.gene061546.t1	MTR_1g069845	47.143	70	37	0	34	103	239	308	2.64e-18	88.2
MS.gene061546.t1	MTR_7g097020	44.595	74	41	0	34	107	239	312	3.28e-18	87.8
MS.gene061546.t1	MTR_2g011140	24.937	397	245	14	21	400	1	361	6.61e-18	85.5
MS.gene061546.t1	MTR_8g080280	39.560	91	49	1	37	127	283	367	6.97e-18	86.7
MS.gene061546.t1	MTR_8g077205	23.789	475	293	14	17	433	146	609	3.48e-17	84.3
MS.gene061546.t1	MTR_8g077205	23.789	475	293	14	17	433	258	721	4.62e-17	84.3
MS.gene061546.t1	MTR_1g079450	25.000	292	190	10	34	314	61	334	2.31e-16	81.3
MS.gene061546.t1	MTR_3g466220	41.892	74	43	0	34	107	231	304	4.33e-15	78.2
MS.gene061546.t1	MTR_3g466220	41.892	74	43	0	34	107	210	283	4.71e-15	78.2
MS.gene061546.t1	MTR_2g096850	45.070	71	39	0	32	102	585	655	1.20e-14	76.6
MS.gene061546.t1	MTR_2g096850	45.070	71	39	0	32	102	736	806	1.71e-14	76.3
MS.gene061546.t1	MTR_5g020570	38.372	86	49	2	32	117	269	350	1.99e-14	75.9
MS.gene061546.t1	MTR_5g020570	38.372	86	49	2	32	117	284	365	1.99e-14	75.9
MS.gene061546.t1	MTR_7g117890	26.459	257	158	7	40	292	259	488	4.27e-14	75.1
MS.gene061546.t1	MTR_3g096370	72.093	43	12	0	33	75	7	49	5.52e-14	72.8
MS.gene061546.t1	MTR_1g093965	46.875	64	31	1	49	112	1	61	5.94e-14	70.9
MS.gene061546.t1	MTR_1g093995	46.875	64	31	1	49	112	1	61	2.00e-13	69.3
MS.gene061546.t1	MTR_1g090320	40.625	96	48	4	31	118	504	598	5.52e-13	71.6
MS.gene061546.t1	MTR_1g090320	40.625	96	48	4	31	118	506	600	5.67e-13	71.6
MS.gene061546.t1	MTR_8g103227	38.636	88	48	2	22	108	386	468	1.22e-12	70.5
MS.gene061546.t1	MTR_5g032010	34.247	73	48	0	36	108	732	804	4.81e-12	68.6
MS.gene061546.t1	MTR_3g115670	24.399	291	172	10	40	316	34	290	5.13e-12	68.6
MS.gene061546.t1	MTR_1g100820	36.364	66	42	0	37	102	649	714	1.42e-11	67.0
MS.gene061546.t1	MTR_3g008270	43.939	66	36	1	37	102	731	795	1.43e-11	67.0
MS.gene061546.t1	MTR_1g100820	36.364	66	42	0	37	102	647	712	1.43e-11	67.0
MS.gene061546.t1	MTR_8g027140	41.176	68	40	0	40	107	263	330	9.32e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061546.t1	AT5G37490	45.894	414	207	8	33	438	28	432	1.09e-110	333
MS.gene061546.t1	AT1G66160	41.346	416	217	9	33	438	30	428	2.39e-101	309
MS.gene061546.t1	AT1G66160	38.942	416	211	10	33	438	30	412	1.47e-89	278
MS.gene061546.t1	AT3G02840	42.486	346	180	7	107	438	36	376	8.89e-77	244
MS.gene061546.t1	AT2G35930	35.154	421	248	8	27	436	3	409	4.62e-64	212
MS.gene061546.t1	AT3G19380	32.990	388	230	12	34	406	12	384	8.06e-54	185
MS.gene061546.t1	AT1G49780	33.417	398	241	11	34	418	12	398	8.86e-54	185
MS.gene061546.t1	AT3G11840	32.159	454	248	13	32	437	6	447	8.49e-47	167
MS.gene061546.t1	AT5G09800	28.101	395	257	12	35	418	10	388	8.28e-34	131
MS.gene061546.t1	AT2G28830	27.230	426	268	14	16	424	166	566	2.38e-33	132
MS.gene061546.t1	AT5G64660	27.206	408	258	13	34	418	8	399	4.58e-33	129
MS.gene061546.t1	AT3G46510	26.209	393	255	12	36	416	256	625	2.02e-32	130
MS.gene061546.t1	AT3G49810	25.700	393	264	6	36	423	64	433	1.66e-31	125
MS.gene061546.t1	AT1G23030	26.617	402	249	14	32	418	237	607	3.44e-31	126
MS.gene061546.t1	AT1G71020	26.877	413	255	14	26	418	85	470	5.80e-31	124
MS.gene061546.t1	AT1G71020	26.877	413	255	14	26	418	233	618	1.88e-30	124
MS.gene061546.t1	AT5G65920	25.570	395	266	6	36	425	60	431	1.06e-29	120
MS.gene061546.t1	AT5G65920	25.570	395	266	6	36	425	60	431	1.06e-29	120
MS.gene061546.t1	AT3G18710	26.238	404	261	12	34	418	10	395	3.73e-27	112
MS.gene061546.t1	AT3G54850	26.750	400	251	14	36	419	248	621	2.06e-26	112
MS.gene061546.t1	AT5G42340	24.242	264	182	6	34	293	288	537	3.97e-22	99.8
MS.gene061546.t1	AT3G07360	50.000	76	38	0	31	106	69	144	2.26e-20	93.6
MS.gene061546.t1	AT1G29340	44.037	109	53	3	34	134	303	411	7.46e-20	92.8
MS.gene061546.t1	AT1G10560	28.024	339	211	14	42	362	294	617	2.53e-19	91.3
MS.gene061546.t1	AT4G21350	25.962	312	198	10	36	339	5	291	6.14e-19	88.6
MS.gene061546.t1	AT5G01830	27.740	292	174	9	30	306	268	537	1.05e-18	89.4
MS.gene061546.t1	AT1G27910	41.758	91	47	1	37	127	280	364	1.19e-18	89.4
MS.gene061546.t1	AT1G60190	25.145	346	232	11	40	370	282	615	3.30e-18	87.8
MS.gene061546.t1	AT3G54790	49.315	73	37	0	34	106	200	272	5.47e-18	87.0
MS.gene061546.t1	AT3G54790	49.315	73	37	0	34	106	236	308	7.00e-18	86.7
MS.gene061546.t1	AT3G54790	49.315	73	37	0	34	106	236	308	7.00e-18	86.7
MS.gene061546.t1	AT1G67530	45.000	80	40	1	37	116	273	348	9.11e-18	85.9
MS.gene061546.t1	AT1G24330	40.659	91	48	1	37	127	276	360	1.13e-17	86.3
MS.gene061546.t1	AT1G24330	40.659	91	48	1	37	127	276	360	1.13e-17	86.3
MS.gene061546.t1	AT1G67530	45.000	80	40	1	37	116	273	348	1.18e-17	86.3
MS.gene061546.t1	AT1G67530	45.000	80	40	1	37	116	273	348	1.18e-17	86.3
MS.gene061546.t1	AT1G67530	45.000	80	40	1	37	116	273	348	1.18e-17	86.3
MS.gene061546.t1	AT5G67340	44.737	76	42	0	34	109	238	313	1.33e-16	82.8
MS.gene061546.t1	AT5G67340	44.737	76	42	0	34	109	238	313	1.33e-16	82.8
MS.gene061546.t1	AT3G52450	33.146	178	111	2	263	436	260	433	2.73e-16	80.9
MS.gene061546.t1	AT4G36550	40.278	72	43	0	35	106	218	289	3.99e-15	78.2
MS.gene061546.t1	AT1G01660	35.556	90	57	1	18	107	481	569	7.32e-15	77.0
MS.gene061546.t1	AT1G01660	35.556	90	57	1	18	107	480	568	7.57e-15	77.0
MS.gene061546.t1	AT2G45920	41.096	73	43	0	37	109	326	398	8.61e-15	76.3
MS.gene061546.t1	AT1G01680	38.571	70	43	0	40	109	237	306	1.02e-14	75.1
MS.gene061546.t1	AT2G23140	35.714	98	59	1	34	131	231	324	2.17e-14	75.9
MS.gene061546.t1	AT2G23140	35.714	98	59	1	34	131	228	321	2.24e-14	75.9
MS.gene061546.t1	AT2G23140	35.714	98	59	1	34	131	228	321	2.24e-14	75.9
MS.gene061546.t1	AT2G23140	37.000	100	57	2	34	131	39	134	3.94e-14	75.1
MS.gene061546.t1	AT2G23140	37.000	100	57	2	34	131	39	134	3.94e-14	75.1
MS.gene061546.t1	AT5G57035	40.909	66	39	0	37	102	709	774	1.05e-13	73.9
MS.gene061546.t1	AT1G56030	42.857	70	40	0	31	100	318	387	4.37e-13	70.9
MS.gene061546.t1	AT5G51270	44.286	70	38	1	37	106	736	804	6.19e-13	71.2
MS.gene061546.t1	AT5G51270	44.286	70	38	1	37	106	736	804	6.19e-13	71.2
MS.gene061546.t1	AT5G65500	40.299	67	40	0	36	102	715	781	6.82e-13	71.2
MS.gene061546.t1	AT5G65500	40.299	67	40	0	36	102	723	789	6.86e-13	71.2
MS.gene061546.t1	AT2G19410	43.939	66	36	1	37	102	712	776	7.98e-13	71.2
MS.gene061546.t1	AT2G19410	43.939	66	36	1	37	102	747	811	8.14e-13	71.2
MS.gene061546.t1	AT2G19410	43.939	66	36	1	37	102	726	790	8.19e-13	70.9
MS.gene061546.t1	AT3G61390	37.879	66	41	0	37	102	279	344	3.50e-12	68.2
MS.gene061546.t1	AT3G61390	37.879	66	41	0	37	102	354	419	4.66e-12	68.2
MS.gene061546.t1	AT3G49060	37.879	66	41	0	37	102	726	791	5.51e-12	68.6
MS.gene061546.t1	AT3G49060	37.879	66	41	0	37	102	736	801	5.69e-12	68.6
MS.gene061546.t1	AT3G49060	37.879	66	41	0	37	102	703	768	6.04e-12	68.2
MS.gene061546.t1	AT1G56040	40.625	64	38	0	37	100	354	417	1.20e-11	66.6
MS.gene061546.t1	AT4G25160	40.909	66	38	1	37	102	767	831	7.82e-11	64.7

Find 75 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAATTGTTGATGGAGAAAA+AGG	0.166972	3.2:+60485694	MS.gene061546:CDS
GAAAGTTTCTGATTTGTAAC+TGG	0.278770	3.2:-60484992	None:intergenic
TAGTGAAAAGTTTGTTGAAT+TGG	0.291875	3.2:+60485648	MS.gene061546:CDS
TGAGGAACATGTTGTTGTTT+TGG	0.298411	3.2:+60485321	MS.gene061546:CDS
ATTCCTGTTGATAGAGTTAC+TGG	0.298592	3.2:-60484914	None:intergenic
CATCTTTGAATTGTTTAGTT+TGG	0.299040	3.2:+60485422	MS.gene061546:CDS
AGGAATAAGAGGGTTATAAT+AGG	0.322167	3.2:+60485241	MS.gene061546:CDS
AGTGAAAAGTTTGTTGAATT+GGG	0.337948	3.2:+60485649	MS.gene061546:CDS
TGAGTTTGTTGGAAAGATTA+AGG	0.348264	3.2:+60485198	MS.gene061546:CDS
TTGCTTGAAACAATTGTTGA+TGG	0.366375	3.2:+60485685	MS.gene061546:CDS
GTTGTCGGGTTGTAGAAATT+TGG	0.371187	3.2:+60485159	MS.gene061546:CDS
TGTGTTGAAAATAGTTCTTA+TGG	0.375679	3.2:+60485067	MS.gene061546:CDS
TGAAGGTGTTGTTGAGGGTT+TGG	0.377823	3.2:+60485528	MS.gene061546:CDS
GAGAGAGTGAGAGGAATAAG+AGG	0.381694	3.2:+60485230	MS.gene061546:CDS
AAGATTGTTAAGGAACCAAT+TGG	0.381968	3.2:+60485553	MS.gene061546:CDS
GACTCACCGGACAACGGAAA+TGG	0.385326	3.2:-60484874	None:intergenic
GAAGATGCTTTGTGAGAAGA+AGG	0.393306	3.2:+60485873	MS.gene061546:CDS
TAATTCGCCGTCGCCGGCGC+CGG	0.395081	3.2:-60484825	None:intergenic
CGCCGGCGACGGCGAATTAA+CGG	0.401684	3.2:+60484829	MS.gene061546:CDS
AAGAGGGTTATAATAGGAAA+TGG	0.402171	3.2:+60485247	MS.gene061546:CDS
TTTGTAGCACTTGACCCAAT+TGG	0.406484	3.2:-60485568	None:intergenic
CCCAACCCATTTCCGTTGTC+CGG	0.411042	3.2:+60484868	MS.gene061546:CDS
CACCGTTAATTCGCCGTCGC+CGG	0.412333	3.2:-60484831	None:intergenic
TGTTTATGTGATTGTAAGAA+AGG	0.414472	3.2:+60485748	MS.gene061546:CDS
TGTTGGTTATGTTGCAAGTT+GGG	0.414797	3.2:+60485944	MS.gene061546:CDS
AAAGCAAAACACCAACTTCC+CGG	0.416680	3.2:+60484806	MS.gene061546:CDS
AATTCGCCGTCGCCGGCGCC+GGG	0.439130	3.2:-60484824	None:intergenic
GTGTTGAAAATAGTTCTTAT+GGG	0.440410	3.2:+60485068	MS.gene061546:CDS
TGAAAAGGCACTAGGTGTAT+TGG	0.441241	3.2:+60485723	MS.gene061546:CDS
TTAACCTCGTACGCTGAAAC+AGG	0.447425	3.2:-60485118	None:intergenic
CGTCGCCGGCGCCGGGAAGT+TGG	0.449959	3.2:-60484817	None:intergenic
TTGGAAAGATTAAGGTTTGG+TGG	0.456739	3.2:+60485206	MS.gene061546:CDS
GGTTTGTACTAGGTTGTTGT+CGG	0.456977	3.2:+60485144	MS.gene061546:CDS
CCGGACAACGGAAATGGGTT+GGG	0.459441	3.2:-60484868	None:intergenic
GATCCAGTAACTCTATCAAC+AGG	0.465887	3.2:+60484911	MS.gene061546:CDS
GGGTTTGGTGAAGATTGTTA+AGG	0.467901	3.2:+60485543	MS.gene061546:CDS
AGAATTGAAGGTGTTGTTGA+GGG	0.472391	3.2:+60485523	MS.gene061546:CDS
ATAGATTCAGCATCGAGAAA+TGG	0.477438	3.2:+60484945	MS.gene061546:CDS
AGGTTAATGAGGTTTGTACT+AGG	0.482538	3.2:+60485134	MS.gene061546:CDS
ACTCACCGGACAACGGAAAT+GGG	0.483936	3.2:-60484873	None:intergenic
GAAGAGTTATCAAGAATTGA+AGG	0.485697	3.2:+60485511	MS.gene061546:CDS
ACCGGACAACGGAAATGGGT+TGG	0.495073	3.2:-60484869	None:intergenic
GTTTATGTGATTGTAAGAAA+GGG	0.496421	3.2:+60485749	MS.gene061546:CDS
TTGTTGGAAAGATTAAGGTT+TGG	0.496940	3.2:+60485203	MS.gene061546:CDS
AGAGAGTGAGAGGAATAAGA+GGG	0.507848	3.2:+60485231	MS.gene061546:CDS
ATCGAGAAATGGATCGAAGC+TGG	0.513343	3.2:+60484956	MS.gene061546:CDS
TTGTTGGTTATGTTGCAAGT+TGG	0.518220	3.2:+60485943	MS.gene061546:CDS
CAACTTCCCGGCGCCGGCGA+CGG	0.518938	3.2:+60484818	MS.gene061546:CDS
TTTGGTGGAGAGAGAGTGAG+AGG	0.525448	3.2:+60485221	MS.gene061546:CDS
GTTTGTACTAGGTTGTTGTC+GGG	0.528503	3.2:+60485145	MS.gene061546:CDS
AAAGTTTCTGATTTGTAACT+GGG	0.529983	3.2:-60484991	None:intergenic
AGAAACATGTTCCGCCGCAC+CGG	0.531078	3.2:+60484782	MS.gene061546:CDS
AAAAGCTTCTTAATCACAAG+AGG	0.544112	3.2:-60485805	None:intergenic
GAGTTGTTGAAATTGTTGAA+TGG	0.546361	3.2:+60485997	MS.gene061546:CDS
AAACACCAACTTCCCGGCGC+CGG	0.546949	3.2:+60484812	MS.gene061546:CDS
TTGATTGAAGCAATTCAACT+TGG	0.549684	3.2:+60485907	MS.gene061546:CDS
AAGAATTGAAGGTGTTGTTG+AGG	0.552708	3.2:+60485522	MS.gene061546:CDS
TTCAGCGTACGAGGTTAATG+AGG	0.552963	3.2:+60485123	MS.gene061546:CDS
GAAACAGGTATACGAGGTGT+TGG	0.554990	3.2:-60485103	None:intergenic
GTGATTTGGTGTGATTTCGA+AGG	0.579587	3.2:-60485017	None:intergenic
TTCATCAAGTCTAGACTCAC+CGG	0.580014	3.2:-60484887	None:intergenic
AGATTGTTAAGGAACCAATT+GGG	0.580879	3.2:+60485554	MS.gene061546:CDS
CGACGGCGAATTAACGGTGG+AGG	0.584194	3.2:+60484835	MS.gene061546:CDS
TATGTTGCAAGTTGGGTGTG+AGG	0.588109	3.2:+60485951	MS.gene061546:CDS
AAGGGAAAGAGGTTGTTCAA+AGG	0.593560	3.2:+60485767	MS.gene061546:CDS
TGATTGTAAGAAAGGGAAAG+AGG	0.594131	3.2:+60485756	MS.gene061546:CDS
TATACCTGTTTCAGCGTACG+AGG	0.604860	3.2:+60485114	MS.gene061546:CDS
CTCGATGCTGAATCTATCGT+AGG	0.614355	3.2:-60484939	None:intergenic
GGGATATGTGAAAAGGCACT+AGG	0.622666	3.2:+60485715	MS.gene061546:CDS
ACAGCAAAACTAGAAGCCAA+AGG	0.636688	3.2:-60485838	None:intergenic
AGTCTAGACTCACCGGACAA+CGG	0.656680	3.2:-60484880	None:intergenic
TGGGTGTGAGGAGAAAACAA+AGG	0.673609	3.2:+60485963	MS.gene061546:CDS
GTGTGAGGAGAAAACAAAGG+AGG	0.681227	3.2:+60485966	MS.gene061546:CDS
TACGCTGAAACAGGTATACG+AGG	0.716974	3.2:-60485109	None:intergenic
CGGCGACGGCGAATTAACGG+TGG	0.722796	3.2:+60484832	MS.gene061546:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTAGATGGTAAAGATTT+AGG	+	chr3.2:60485445-60485464	MS.gene061546:CDS	20.0%
!	CATCTTTGAATTGTTTAGTT+TGG	+	chr3.2:60485422-60485441	MS.gene061546:CDS	25.0%
!	GTGTTGAAAATAGTTCTTAT+GGG	+	chr3.2:60485068-60485087	MS.gene061546:CDS	25.0%
!	GTTTATGTGATTGTAAGAAA+GGG	+	chr3.2:60485749-60485768	MS.gene061546:CDS	25.0%
!	TAAGAAAAATGATTCAGAGT+TGG	+	chr3.2:60485044-60485063	MS.gene061546:CDS	25.0%
!	TGTGTTGAAAATAGTTCTTA+TGG	+	chr3.2:60485067-60485086	MS.gene061546:CDS	25.0%
!	TGTTTATGTGATTGTAAGAA+AGG	+	chr3.2:60485748-60485767	MS.gene061546:CDS	25.0%
!!	AAAGTTTCTGATTTGTAACT+GGG	-	chr3.2:60484994-60485013	None:intergenic	25.0%
!!	ATTTTTCTTATGGTGTGATT+TGG	-	chr3.2:60485034-60485053	None:intergenic	25.0%
!!	TTTTTCATGTGTTTCTTTTG+AGG	+	chr3.2:60485303-60485322	MS.gene061546:CDS	25.0%
!!!	AATTTTCAAGTTGGTTTCAT+CGG	+	chr3.2:60485606-60485625	MS.gene061546:CDS	25.0%
!!!	ACTCTGAATCATTTTTCTTA+TGG	-	chr3.2:60485044-60485063	None:intergenic	25.0%
!!!	AGATTTTGGAGATTTTAACA+TGG	+	chr3.2:60485347-60485366	MS.gene061546:CDS	25.0%
!!!	AGTGAAAAGTTTGTTGAATT+GGG	+	chr3.2:60485649-60485668	MS.gene061546:CDS	25.0%
!!!	TAGTGAAAAGTTTGTTGAAT+TGG	+	chr3.2:60485648-60485667	MS.gene061546:CDS	25.0%
!!!	TGTTTAGTTTGGTTTTTAGA+TGG	+	chr3.2:60485433-60485452	MS.gene061546:CDS	25.0%
!!!	TTTGACTACAATTTTCAAGT+TGG	+	chr3.2:60485597-60485616	MS.gene061546:CDS	25.0%
	AAAAGCTTCTTAATCACAAG+AGG	-	chr3.2:60485808-60485827	None:intergenic	30.0%
	AAGAGGGTTATAATAGGAAA+TGG	+	chr3.2:60485247-60485266	MS.gene061546:CDS	30.0%
	AAGATTGTTAAGGAACCAAT+TGG	+	chr3.2:60485553-60485572	MS.gene061546:CDS	30.0%
	AGAAAAAGGGATATGTGAAA+AGG	+	chr3.2:60485708-60485727	MS.gene061546:CDS	30.0%
	AGATTGTTAAGGAACCAATT+GGG	+	chr3.2:60485554-60485573	MS.gene061546:CDS	30.0%
	AGGAATAAGAGGGTTATAAT+AGG	+	chr3.2:60485241-60485260	MS.gene061546:CDS	30.0%
	GAAGAGTTATCAAGAATTGA+AGG	+	chr3.2:60485511-60485530	MS.gene061546:CDS	30.0%
	GAGTTGTTGAAATTGTTGAA+TGG	+	chr3.2:60485997-60486016	MS.gene061546:CDS	30.0%
	TTGATTGAAGCAATTCAACT+TGG	+	chr3.2:60485907-60485926	MS.gene061546:CDS	30.0%
	TTGCTTGAAACAATTGTTGA+TGG	+	chr3.2:60485685-60485704	MS.gene061546:CDS	30.0%
	TTGTTGGAAAGATTAAGGTT+TGG	+	chr3.2:60485203-60485222	MS.gene061546:CDS	30.0%
!	AAAAAGTGTGTTGAGTTTGT+TGG	+	chr3.2:60485187-60485206	MS.gene061546:CDS	30.0%
!	ACAATTGTTGATGGAGAAAA+AGG	+	chr3.2:60485694-60485713	MS.gene061546:CDS	30.0%
!	CAATTGTTGATGGAGAAAAA+GGG	+	chr3.2:60485695-60485714	MS.gene061546:CDS	30.0%
!	GAAAGTTTCTGATTTGTAAC+TGG	-	chr3.2:60484995-60485014	None:intergenic	30.0%
!	TGTGATTAAGAAGCTTTTGA+GGG	+	chr3.2:60485810-60485829	MS.gene061546:CDS	30.0%
!	TTGTGATTAAGAAGCTTTTG+AGG	+	chr3.2:60485809-60485828	MS.gene061546:CDS	30.0%
!!	TGAGTTTGTTGGAAAGATTA+AGG	+	chr3.2:60485198-60485217	MS.gene061546:CDS	30.0%
!!!	AGTTTGTTCTGTTTTAGCTA+CGG	+	chr3.2:60485270-60485289	MS.gene061546:CDS	30.0%
	AGGTTAATGAGGTTTGTACT+AGG	+	chr3.2:60485134-60485153	MS.gene061546:CDS	35.0%
	TGATTGTAAGAAAGGGAAAG+AGG	+	chr3.2:60485756-60485775	MS.gene061546:CDS	35.0%
	TTGGAAAGATTAAGGTTTGG+TGG	+	chr3.2:60485206-60485225	MS.gene061546:CDS	35.0%
!	ATAGATTCAGCATCGAGAAA+TGG	+	chr3.2:60484945-60484964	MS.gene061546:CDS	35.0%
!	ATTCCTGTTGATAGAGTTAC+TGG	-	chr3.2:60484917-60484936	None:intergenic	35.0%
!	TGGTGCTTTTCAGAAATTGT+TGG	+	chr3.2:60485927-60485946	MS.gene061546:CDS	35.0%
!	TGTTGGTTATGTTGCAAGTT+GGG	+	chr3.2:60485944-60485963	MS.gene061546:CDS	35.0%
!	TTGTTGGTTATGTTGCAAGT+TGG	+	chr3.2:60485943-60485962	MS.gene061546:CDS	35.0%
!!	AAGAATTGAAGGTGTTGTTG+AGG	+	chr3.2:60485522-60485541	MS.gene061546:CDS	35.0%
!!	AGAATTGAAGGTGTTGTTGA+GGG	+	chr3.2:60485523-60485542	MS.gene061546:CDS	35.0%
!!!	TGAGGAACATGTTGTTGTTT+TGG	+	chr3.2:60485321-60485340	MS.gene061546:CDS	35.0%
!!!	TGTTGTTTTGGAGGAGATTT+TGG	+	chr3.2:60485333-60485352	MS.gene061546:CDS	35.0%
	AAAGCAAAACACCAACTTCC+CGG	+	chr3.2:60484806-60484825	MS.gene061546:CDS	40.0%
	AAGGGAAAGAGGTTGTTCAA+AGG	+	chr3.2:60485767-60485786	MS.gene061546:CDS	40.0%
	ACAGCAAAACTAGAAGCCAA+AGG	-	chr3.2:60485841-60485860	None:intergenic	40.0%
	AGAGAGTGAGAGGAATAAGA+GGG	+	chr3.2:60485231-60485250	MS.gene061546:CDS	40.0%
	GAAGATGCTTTGTGAGAAGA+AGG	+	chr3.2:60485873-60485892	MS.gene061546:CDS	40.0%
	GATCCAGTAACTCTATCAAC+AGG	+	chr3.2:60484911-60484930	MS.gene061546:CDS	40.0%
	GGTTTGTACTAGGTTGTTGT+CGG	+	chr3.2:60485144-60485163	MS.gene061546:CDS	40.0%
	GTTTGTACTAGGTTGTTGTC+GGG	+	chr3.2:60485145-60485164	MS.gene061546:CDS	40.0%
	TTCATCAAGTCTAGACTCAC+CGG	-	chr3.2:60484890-60484909	None:intergenic	40.0%
	TTTGTAGCACTTGACCCAAT+TGG	-	chr3.2:60485571-60485590	None:intergenic	40.0%
!	CGTTTTCGAATCGCTAAAAG+AGG	-	chr3.2:60485383-60485402	None:intergenic	40.0%
!	GTTGTCGGGTTGTAGAAATT+TGG	+	chr3.2:60485159-60485178	MS.gene061546:CDS	40.0%
!!	GGGTTTGGTGAAGATTGTTA+AGG	+	chr3.2:60485543-60485562	MS.gene061546:CDS	40.0%
!!	GTGATTTGGTGTGATTTCGA+AGG	-	chr3.2:60485020-60485039	None:intergenic	40.0%
!!	TGAAAAGGCACTAGGTGTAT+TGG	+	chr3.2:60485723-60485742	MS.gene061546:CDS	40.0%
!!!	AAGTTGGTGTTTTGCTTTGC+CGG	-	chr3.2:60484804-60484823	None:intergenic	40.0%
!!!	GGAACATGTTGTTGTTTTGG+AGG	+	chr3.2:60485324-60485343	MS.gene061546:CDS	40.0%
!!!	GTTTTGCTGTTGGGATTGTT+AGG	+	chr3.2:60485851-60485870	MS.gene061546:CDS	40.0%
!!!	TGGCTTCTAGTTTTGCTGTT+GGG	+	chr3.2:60485842-60485861	MS.gene061546:CDS	40.0%
!!!	TTGGCTTCTAGTTTTGCTGT+TGG	+	chr3.2:60485841-60485860	MS.gene061546:CDS	40.0%
	ATCGAGAAATGGATCGAAGC+TGG	+	chr3.2:60484956-60484975	MS.gene061546:CDS	45.0%
	CTCGATGCTGAATCTATCGT+AGG	-	chr3.2:60484942-60484961	None:intergenic	45.0%
	GAAACAGGTATACGAGGTGT+TGG	-	chr3.2:60485106-60485125	None:intergenic	45.0%
	GAGAGAGTGAGAGGAATAAG+AGG	+	chr3.2:60485230-60485249	MS.gene061546:CDS	45.0%
	GTGTGAGGAGAAAACAAAGG+AGG	+	chr3.2:60485966-60485985	MS.gene061546:CDS	45.0%
	TACGCTGAAACAGGTATACG+AGG	-	chr3.2:60485112-60485131	None:intergenic	45.0%
	TATACCTGTTTCAGCGTACG+AGG	+	chr3.2:60485114-60485133	MS.gene061546:CDS	45.0%
	TGGGTGTGAGGAGAAAACAA+AGG	+	chr3.2:60485963-60485982	MS.gene061546:CDS	45.0%
	TTAACCTCGTACGCTGAAAC+AGG	-	chr3.2:60485121-60485140	None:intergenic	45.0%
	TTCAGCGTACGAGGTTAATG+AGG	+	chr3.2:60485123-60485142	MS.gene061546:CDS	45.0%
!	TATGTTGCAAGTTGGGTGTG+AGG	+	chr3.2:60485951-60485970	MS.gene061546:CDS	45.0%
!!	GGGATATGTGAAAAGGCACT+AGG	+	chr3.2:60485715-60485734	MS.gene061546:CDS	45.0%
!!	TGAAGGTGTTGTTGAGGGTT+TGG	+	chr3.2:60485528-60485547	MS.gene061546:CDS	45.0%
	ACTCACCGGACAACGGAAAT+GGG	-	chr3.2:60484876-60484895	None:intergenic	50.0%
	AGTCTAGACTCACCGGACAA+CGG	-	chr3.2:60484883-60484902	None:intergenic	50.0%
	TTTGGTGGAGAGAGAGTGAG+AGG	+	chr3.2:60485221-60485240	MS.gene061546:CDS	50.0%
!!!	GCTTTTGAGGGTGTCTCCTT+TGG	+	chr3.2:60485822-60485841	MS.gene061546:CDS	50.0%
	ACCGGACAACGGAAATGGGT+TGG	-	chr3.2:60484872-60484891	None:intergenic	55.0%
	AGAAACATGTTCCGCCGCAC+CGG	+	chr3.2:60484782-60484801	MS.gene061546:CDS	55.0%
	CCCAACCCATTTCCGTTGTC+CGG	+	chr3.2:60484868-60484887	MS.gene061546:CDS	55.0%
	GACTCACCGGACAACGGAAA+TGG	-	chr3.2:60484877-60484896	None:intergenic	55.0%
!	CCGGACAACGGAAATGGGTT+GGG	-	chr3.2:60484871-60484890	None:intergenic	55.0%
!	GGTGTTTTGCTTTGCCGGTG+CGG	-	chr3.2:60484799-60484818	None:intergenic	55.0%
	AAACACCAACTTCCCGGCGC+CGG	+	chr3.2:60484812-60484831	MS.gene061546:CDS	60.0%
	CACCGTTAATTCGCCGTCGC+CGG	-	chr3.2:60484834-60484853	None:intergenic	60.0%
	CGACGGCGAATTAACGGTGG+AGG	+	chr3.2:60484835-60484854	MS.gene061546:CDS	60.0%
!	GTTTTGCTTTGCCGGTGCGG+CGG	-	chr3.2:60484796-60484815	None:intergenic	60.0%
	CGCCGGCGACGGCGAATTAA+CGG	+	chr3.2:60484829-60484848	MS.gene061546:CDS	65.0%
	CGGCGACGGCGAATTAACGG+TGG	+	chr3.2:60484832-60484851	MS.gene061546:CDS	65.0%
	TAATTCGCCGTCGCCGGCGC+CGG	-	chr3.2:60484828-60484847	None:intergenic	70.0%
	AATTCGCCGTCGCCGGCGCC+GGG	-	chr3.2:60484827-60484846	None:intergenic	75.0%
!!	CAACTTCCCGGCGCCGGCGA+CGG	+	chr3.2:60484818-60484837	MS.gene061546:CDS	75.0%
!!	CGTCGCCGGCGCCGGGAAGT+TGG	-	chr3.2:60484820-60484839	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	60484761	60486080	60484761	ID=MS.gene061546
chr3.2	mRNA	60484761	60486080	60484761	ID=MS.gene061546.t1;Parent=MS.gene061546
chr3.2	exon	60484761	60486080	60484761	ID=MS.gene061546.t1.exon1;Parent=MS.gene061546.t1
chr3.2	CDS	60484761	60486080	60484761	ID=cds.MS.gene061546.t1;Parent=MS.gene061546.t1

Gene Sequence

>MS.gene061546

ATGGTTTTATCATGGACAAAAAGAAACATGTTCCGCCGCACCGGCAAAGCAAAACACCAACTTCCCGGCGCCGGCGACGGCGAATTAACGGTGGAGGAGATAACAATCCCAACCCATTTCCGTTGTCCGGTGAGTCTAGACTTGATGAAAGATCCAGTAACTCTATCAACAGGAATCACCTACGATAGATTCAGCATCGAGAAATGGATCGAAGCTGGAAACAAAACTTGCCCAGTTACAAATCAGAAACTTTCAACCTTCGAAATCACACCAAATCACACCATAAGAAAAATGATTCAGAGTTGGTGTGTTGAAAATAGTTCTTATGGGATTGAGAGAATTCCAACACCTCGTATACCTGTTTCAGCGTACGAGGTTAATGAGGTTTGTACTAGGTTGTTGTCGGGTTGTAGAAATTTGGATGAGAAAAAGTGTGTTGAGTTTGTTGGAAAGATTAAGGTTTGGTGGAGAGAGAGTGAGAGGAATAAGAGGGTTATAATAGGAAATGGAGTTTGTTCTGTTTTAGCTACGGTTTTTGATTGTTTTTCATGTGTTTCTTTTGAGGAACATGTTGTTGTTTTGGAGGAGATTTTGGAGATTTTAACATGGATTGTTAAAACCTCTTTTAGCGATTCGAAAACGAAGATGTGTTTATCGTCTTCATCTTTGAATTGTTTAGTTTGGTTTTTAGATGGTAAAGATTTAGGTGCAAGACAAAATGCTGTTTTGTTGCTTAAAGAAATGAATGTTGAAGAGTTATCAAGAATTGAAGGTGTTGTTGAGGGTTTGGTGAAGATTGTTAAGGAACCAATTGGGTCAAGTGCTACAAAAGCATGTTTGACTACAATTTTCAAGTTGGTTTCATCGGCGAAAAATAGAGATGAGATTAGTGAAAAGTTTGTTGAATTGGGTTTGATTTCATTTTTGCTTGAAACAATTGTTGATGGAGAAAAAGGGATATGTGAAAAGGCACTAGGTGTATTGGATTGTTTATGTGATTGTAAGAAAGGGAAAGAGGTTGTTCAAAGGAACGCTTTAGCTTTGCCTCTTGTGATTAAGAAGCTTTTGAGGGTGTCTCCTTTGGCTTCTAGTTTTGCTGTTGGGATTGTTAGGAAGATGCTTTGTGAGAAGAAGGAAGAAAGAGTTTTGATTGAAGCAATTCAACTTGGTGCTTTTCAGAAATTGTTGGTTATGTTGCAAGTTGGGTGTGAGGAGAAAACAAAGGAGGATACTACTGAGTTGTTGAAATTGTTGAATGGTTATAGAAGCAAAGCTGAATGTGTTGACAATTCATTAGATTTCAAGTATCTCAAGAACTAA

Protein sequence

>MS.gene061546.t1

MVLSWTKRNMFRRTGKAKHQLPGAGDGELTVEEITIPTHFRCPVSLDLMKDPVTLSTGITYDRFSIEKWIEAGNKTCPVTNQKLSTFEITPNHTIRKMIQSWCVENSSYGIERIPTPRIPVSAYEVNEVCTRLLSGCRNLDEKKCVEFVGKIKVWWRESERNKRVIIGNGVCSVLATVFDCFSCVSFEEHVVVLEEILEILTWIVKTSFSDSKTKMCLSSSSLNCLVWFLDGKDLGARQNAVLLLKEMNVEELSRIEGVVEGLVKIVKEPIGSSATKACLTTIFKLVSSAKNRDEISEKFVELGLISFLLETIVDGEKGICEKALGVLDCLCDCKKGKEVVQRNALALPLVIKKLLRVSPLASSFAVGIVRKMLCEKKEERVLIEAIQLGAFQKLLVMLQVGCEEKTKEDTTELLKLLNGYRSKAECVDNSLDFKYLKN