AlfalfaGEDB | MS.gene017373

M. sativa cultivar XinJiangDaYe / MS.gene017373

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017373.t1	XP_003616222.1	96.6	442	15	0	1	442	1	442	2.80E-237	830.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017373.t1	Q9C8D1	44.0	434	214	9	19	442	17	431	7.9e-95	349.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017373.t1	G7KFZ3	96.6	442	15	0	1	442	1	442	2.0e-237	830.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene017373	MS.gene064492	PPI
MS.gene017373	MS.gene26139	PPI
MS.gene017373	MS.gene72611	PPI
MS.gene017373	MS.gene007121	PPI
MS.gene017373	MS.gene048677	PPI
MS.gene017373	MS.gene066130	PPI
MS.gene017373	MS.gene45454	PPI
MS.gene017373	MS.gene26489	PPI
MS.gene017373	MS.gene022190	PPI
MS.gene017373	MS.gene45455	PPI
MS.gene017373	MS.gene042912	PPI
MS.gene034751	MS.gene017373	PPI
MS.gene034753	MS.gene017373	PPI
MS.gene017373	MS.gene066129	PPI
MS.gene017373	MS.gene061546	PPI
MS.gene41032	MS.gene017373	PPI
MS.gene017373	MS.gene41033	PPI
MS.gene04998	MS.gene017373	PPI
MS.gene017373	MS.gene008306	PPI
MS.gene45456	MS.gene017373	PPI
MS.gene025667	MS.gene017373	PPI
MS.gene017373	MS.gene93972	PPI
MS.gene008306	MS.gene017373	PPI
MS.gene061543	MS.gene017373	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017373.t1	MTR_5g077510	96.606	442	15	0	1	442	1	442	0.0	868
MS.gene017373.t1	MTR_3g065080	63.883	443	151	8	1	439	1	438	0.0	518
MS.gene017373.t1	MTR_6g071340	48.235	425	200	8	31	442	1	418	7.37e-128	376
MS.gene017373.t1	MTR_5g048050	47.887	426	202	8	30	442	20	438	1.45e-125	371
MS.gene017373.t1	MTR_4g085720	33.915	401	235	11	29	418	8	389	3.75e-58	196
MS.gene017373.t1	MTR_7g106340	33.803	426	242	13	32	433	5	414	6.59e-57	193
MS.gene017373.t1	MTR_2g087350	35.452	409	235	12	32	428	3	394	7.32e-55	187
MS.gene017373.t1	MTR_1g056880	33.949	433	239	15	32	428	4	425	3.08e-53	184
MS.gene017373.t1	MTR_1g056870	34.096	437	233	16	32	428	4	425	4.30e-53	184
MS.gene017373.t1	MTR_1g056910	31.401	414	259	11	32	433	3	403	3.94e-52	181
MS.gene017373.t1	MTR_1g056840	32.184	435	218	16	32	428	4	399	1.28e-47	169
MS.gene017373.t1	MTR_4g051515	27.619	420	264	14	32	429	3	404	7.82e-36	137
MS.gene017373.t1	MTR_8g092870	28.341	434	264	19	33	442	8	418	6.89e-34	131
MS.gene017373.t1	MTR_3g095730	27.040	429	274	20	33	442	11	419	3.29e-33	130
MS.gene017373.t1	MTR_5g015210	30.075	399	228	12	35	417	67	430	6.08e-32	127
MS.gene017373.t1	MTR_4g063800	27.340	406	243	16	34	419	254	627	2.08e-30	124
MS.gene017373.t1	MTR_1g017770	28.827	392	247	9	35	417	45	413	2.57e-30	122
MS.gene017373.t1	MTR_4g485520	26.492	419	258	17	22	418	88	478	1.34e-26	112
MS.gene017373.t1	MTR_4g485520	26.238	404	252	16	33	418	271	646	2.46e-26	112
MS.gene017373.t1	MTR_4g107010	28.037	428	267	15	15	425	267	670	4.30e-26	111
MS.gene017373.t1	MTR_3g096370	60.000	75	30	0	27	101	2	76	7.76e-26	108
MS.gene017373.t1	MTR_5g083030	26.087	414	259	13	33	419	291	684	9.73e-25	107
MS.gene017373.t1	MTR_7g059405	26.521	411	235	18	35	419	264	633	1.91e-24	106
MS.gene017373.t1	MTR_2g018010	26.032	315	195	9	33	329	290	584	4.78e-23	102
MS.gene017373.t1	MTR_7g078330	25.246	305	190	10	33	325	272	550	5.03e-23	102
MS.gene017373.t1	MTR_5g034440	26.118	425	277	11	15	424	268	670	2.03e-22	100
MS.gene017373.t1	MTR_4g028960	46.053	76	41	0	31	106	276	351	1.50e-21	97.8
MS.gene017373.t1	MTR_3g078160	27.928	333	205	13	24	340	264	577	5.50e-21	96.3
MS.gene017373.t1	MTR_3g078160	27.928	333	205	13	24	340	264	577	5.71e-21	96.3
MS.gene017373.t1	MTR_3g078160	34.532	139	84	3	24	162	264	395	2.70e-20	94.0
MS.gene017373.t1	MTR_3g085610	41.667	84	43	1	36	119	281	358	3.38e-19	90.9
MS.gene017373.t1	MTR_1g094215	27.356	329	211	10	35	354	64	373	8.40e-19	88.6
MS.gene017373.t1	MTR_1g094215	27.576	330	209	11	35	354	247	556	1.01e-18	89.4
MS.gene017373.t1	MTR_4g091880	48.529	68	34	1	35	101	5	72	1.17e-18	87.8
MS.gene017373.t1	MTR_4g091880	48.529	68	34	1	35	101	5	72	1.25e-18	87.0
MS.gene017373.t1	MTR_1g069845	44.286	70	39	0	33	102	122	191	2.99e-18	87.8
MS.gene017373.t1	MTR_7g097020	37.805	82	51	0	21	102	227	308	3.86e-18	87.4
MS.gene017373.t1	MTR_1g069845	44.286	70	39	0	33	102	243	312	5.49e-18	87.0
MS.gene017373.t1	MTR_1g069845	44.286	70	39	0	33	102	239	308	5.51e-18	87.0
MS.gene017373.t1	MTR_7g116600	29.667	300	178	12	32	316	75	356	1.37e-17	85.1
MS.gene017373.t1	MTR_7g053260	43.478	69	39	0	33	101	76	144	1.25e-16	82.0
MS.gene017373.t1	MTR_1g079450	26.821	302	188	12	30	316	58	341	2.33e-16	81.3
MS.gene017373.t1	MTR_2g011140	39.506	81	49	0	21	101	1	81	2.39e-16	80.9
MS.gene017373.t1	MTR_2g096850	42.857	70	40	0	32	101	737	806	2.03e-15	79.3
MS.gene017373.t1	MTR_8g080280	38.095	84	46	1	36	119	283	360	2.10e-15	79.0
MS.gene017373.t1	MTR_2g096850	42.857	70	40	0	32	101	586	655	2.19e-15	79.0
MS.gene017373.t1	MTR_8g077205	41.667	72	42	0	31	102	159	230	5.56e-15	77.8
MS.gene017373.t1	MTR_8g077205	41.667	72	42	0	31	102	271	342	6.33e-15	77.4
MS.gene017373.t1	MTR_7g077780	40.000	70	42	0	36	105	827	896	1.76e-14	76.3
MS.gene017373.t1	MTR_7g117890	24.138	232	144	4	39	265	259	463	2.57e-14	75.9
MS.gene017373.t1	MTR_3g115670	31.333	150	80	4	39	183	34	165	3.82e-14	75.1
MS.gene017373.t1	MTR_5g032010	33.333	87	58	0	15	101	712	798	4.97e-14	74.7
MS.gene017373.t1	MTR_3g096370	64.444	45	16	0	27	71	2	46	1.45e-13	71.6
MS.gene017373.t1	MTR_5g020570	38.095	84	48	2	33	116	271	350	1.82e-13	73.2
MS.gene017373.t1	MTR_1g100820	39.394	66	40	0	36	101	647	712	1.96e-13	72.8
MS.gene017373.t1	MTR_1g100820	39.394	66	40	0	36	101	649	714	1.97e-13	72.8
MS.gene017373.t1	MTR_5g020570	38.095	84	48	2	33	116	286	365	2.01e-13	72.8
MS.gene017373.t1	MTR_1g090320	41.860	86	45	3	36	117	514	598	2.36e-13	72.8
MS.gene017373.t1	MTR_1g090320	41.860	86	45	3	36	117	516	600	2.40e-13	72.8
MS.gene017373.t1	MTR_3g466220	37.143	70	44	0	33	102	231	300	4.62e-13	71.6
MS.gene017373.t1	MTR_3g466220	37.143	70	44	0	33	102	210	279	4.99e-13	71.6
MS.gene017373.t1	MTR_3g008270	43.939	66	36	1	36	101	731	795	1.92e-12	69.7
MS.gene017373.t1	MTR_7g005940	42.857	70	37	2	34	101	594	662	3.21e-12	69.3
MS.gene017373.t1	MTR_7g005940	42.857	70	37	2	34	101	594	662	3.21e-12	69.3
MS.gene017373.t1	MTR_8g103227	43.284	67	37	1	36	101	396	462	3.23e-12	69.3
MS.gene017373.t1	MTR_1g093965	45.312	64	32	1	48	111	1	61	4.93e-12	65.1
MS.gene017373.t1	MTR_1g093995	45.312	64	32	1	48	111	1	61	6.31e-12	65.1
MS.gene017373.t1	MTR_8g027140	23.707	232	145	6	39	265	263	467	6.63e-12	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017373.t1	AT1G66160	44.270	445	217	11	10	442	6	431	3.34e-122	362
MS.gene017373.t1	AT5G37490	45.215	418	212	8	32	442	28	435	7.04e-113	339
MS.gene017373.t1	AT1G66160	42.022	445	211	12	10	442	6	415	3.14e-109	329
MS.gene017373.t1	AT3G02840	41.573	356	184	10	103	442	32	379	2.65e-72	233
MS.gene017373.t1	AT2G35930	34.750	400	229	11	32	417	9	390	4.55e-60	201
MS.gene017373.t1	AT3G19380	32.737	391	234	10	29	405	8	383	1.95e-58	197
MS.gene017373.t1	AT1G49780	33.582	402	244	10	29	418	8	398	3.16e-55	189
MS.gene017373.t1	AT3G52450	31.178	433	263	9	32	434	4	431	1.48e-53	185
MS.gene017373.t1	AT3G11840	31.066	441	253	12	32	429	7	439	5.80e-44	160
MS.gene017373.t1	AT5G64660	27.465	426	280	17	33	442	8	420	7.01e-34	132
MS.gene017373.t1	AT3G49810	28.535	396	261	6	28	423	60	433	2.54e-33	130
MS.gene017373.t1	AT5G65920	27.366	391	265	4	35	425	60	431	4.20e-32	127
MS.gene017373.t1	AT5G65920	27.366	391	265	4	35	425	60	431	4.20e-32	127
MS.gene017373.t1	AT5G09800	26.536	407	262	13	27	418	4	388	2.25e-30	122
MS.gene017373.t1	AT2G28830	25.432	405	263	14	36	424	185	566	1.05e-26	113
MS.gene017373.t1	AT1G23030	27.047	403	245	15	32	418	238	607	2.65e-26	112
MS.gene017373.t1	AT3G18710	25.850	441	273	14	27	442	4	415	9.35e-25	105
MS.gene017373.t1	AT1G71020	27.427	412	239	18	33	418	93	470	1.71e-24	105
MS.gene017373.t1	AT1G71020	27.427	412	239	18	33	418	241	618	3.13e-24	105
MS.gene017373.t1	AT3G46510	25.750	400	249	15	35	416	256	625	4.18e-24	105
MS.gene017373.t1	AT1G10560	28.313	332	201	9	41	353	294	607	1.60e-22	100
MS.gene017373.t1	AT5G42340	30.625	160	106	2	35	193	290	445	3.27e-22	100
MS.gene017373.t1	AT3G54850	26.448	397	255	14	35	418	248	620	7.55e-22	99.0
MS.gene017373.t1	AT5G01830	52.941	68	32	0	35	102	274	341	4.50e-19	90.5
MS.gene017373.t1	AT1G29340	35.811	148	84	4	33	170	303	449	6.55e-19	90.1
MS.gene017373.t1	AT4G21350	27.184	309	196	10	35	338	5	289	2.74e-18	86.7
MS.gene017373.t1	AT1G60190	25.000	340	227	10	39	365	282	606	1.98e-17	85.5
MS.gene017373.t1	AT1G27910	38.235	102	51	2	36	128	280	378	9.33e-17	83.2
MS.gene017373.t1	AT3G54790	43.243	74	42	0	33	106	200	273	1.07e-16	83.2
MS.gene017373.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.27e-16	82.8
MS.gene017373.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.27e-16	82.8
MS.gene017373.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.27e-16	82.8
MS.gene017373.t1	AT3G54790	43.243	74	42	0	33	106	236	309	1.36e-16	82.8
MS.gene017373.t1	AT3G54790	43.243	74	42	0	33	106	236	309	1.36e-16	82.8
MS.gene017373.t1	AT3G07360	43.478	92	51	1	11	102	51	141	1.56e-16	82.0
MS.gene017373.t1	AT1G67530	41.463	82	47	1	24	105	262	342	2.09e-16	82.0
MS.gene017373.t1	AT1G24330	42.857	70	40	0	36	105	276	345	2.32e-16	82.0
MS.gene017373.t1	AT1G24330	42.857	70	40	0	36	105	276	345	2.32e-16	82.0
MS.gene017373.t1	AT2G45920	32.407	108	59	2	8	101	284	391	2.22e-15	78.2
MS.gene017373.t1	AT4G36550	40.845	71	42	0	35	105	219	289	2.23e-15	79.0
MS.gene017373.t1	AT5G57035	38.372	86	50	1	19	101	689	774	2.62e-15	79.0
MS.gene017373.t1	AT3G49060	36.585	82	52	0	23	104	690	771	1.01e-14	77.0
MS.gene017373.t1	AT3G49060	36.585	82	52	0	23	104	723	804	1.02e-14	77.0
MS.gene017373.t1	AT3G49060	36.585	82	52	0	23	104	713	794	1.10e-14	77.0
MS.gene017373.t1	AT1G01680	35.789	95	60	1	15	108	212	306	1.10e-14	75.1
MS.gene017373.t1	AT1G01660	37.879	66	41	0	36	101	499	564	1.71e-14	75.9
MS.gene017373.t1	AT1G01660	37.879	66	41	0	36	101	498	563	1.77e-14	75.9
MS.gene017373.t1	AT3G61390	42.424	66	38	0	36	101	279	344	2.08e-14	74.7
MS.gene017373.t1	AT3G61390	42.424	66	38	0	36	101	354	419	2.79e-14	75.1
MS.gene017373.t1	AT5G65500	35.227	88	57	0	14	101	694	781	2.33e-13	72.8
MS.gene017373.t1	AT5G65500	35.227	88	57	0	14	101	702	789	2.34e-13	72.8
MS.gene017373.t1	AT2G45910	37.143	70	44	0	36	105	764	833	5.19e-13	71.6
MS.gene017373.t1	AT2G45910	37.143	70	44	0	36	105	764	833	5.19e-13	71.6
MS.gene017373.t1	AT1G20780	45.588	68	32	1	39	101	27	94	9.69e-13	70.9
MS.gene017373.t1	AT1G20780	45.588	68	32	1	39	101	27	94	9.69e-13	70.9
MS.gene017373.t1	AT1G20780	45.588	68	32	1	39	101	27	94	9.69e-13	70.9
MS.gene017373.t1	AT2G23140	38.571	70	43	0	33	102	231	300	9.93e-13	70.9
MS.gene017373.t1	AT2G23140	38.571	70	43	0	33	102	228	297	1.03e-12	70.9
MS.gene017373.t1	AT2G23140	38.571	70	43	0	33	102	228	297	1.03e-12	70.9
MS.gene017373.t1	AT5G51270	43.939	66	36	1	36	101	736	800	1.07e-12	70.9
MS.gene017373.t1	AT5G51270	43.939	66	36	1	36	101	736	800	1.07e-12	70.9
MS.gene017373.t1	AT2G23140	38.571	70	43	0	33	102	39	108	1.37e-12	70.1
MS.gene017373.t1	AT2G23140	38.571	70	43	0	33	102	39	108	1.37e-12	70.1
MS.gene017373.t1	AT1G56040	41.538	65	38	0	36	100	354	418	1.96e-12	69.3
MS.gene017373.t1	AT1G01670	36.000	75	48	0	31	105	206	280	2.18e-12	67.8
MS.gene017373.t1	AT5G67340	35.000	80	52	0	29	108	234	313	2.73e-12	69.3
MS.gene017373.t1	AT5G67340	35.000	80	52	0	29	108	234	313	2.73e-12	69.3
MS.gene017373.t1	AT2G19410	34.884	86	52	2	19	101	706	790	3.59e-12	68.9
MS.gene017373.t1	AT2G19410	34.884	86	52	2	19	101	692	776	3.86e-12	68.9
MS.gene017373.t1	AT2G19410	34.884	86	52	2	19	101	727	811	4.18e-12	68.9
MS.gene017373.t1	AT1G01670	36.000	75	48	0	31	105	290	364	4.33e-12	67.8
MS.gene017373.t1	AT1G01670	36.000	75	48	0	31	105	290	364	4.33e-12	67.8
MS.gene017373.t1	AT1G56030	40.000	65	39	0	36	100	324	388	5.00e-12	67.8
MS.gene017373.t1	AT1G76390	41.379	87	43	3	23	101	10	96	7.23e-12	68.2
MS.gene017373.t1	AT1G76390	41.379	87	43	3	23	101	10	96	7.23e-12	68.2
MS.gene017373.t1	AT4G25160	40.845	71	41	1	31	101	762	831	8.28e-12	67.8
MS.gene017373.t1	AT5G61560	40.580	69	40	1	36	104	726	793	1.31e-11	67.4
MS.gene017373.t1	AT5G61560	40.580	69	40	1	36	104	726	793	1.31e-11	67.4
MS.gene017373.t1	AT5G61560	40.580	69	40	1	36	104	724	791	1.35e-11	67.4
MS.gene017373.t1	AT5G61560	40.580	69	40	1	36	104	745	812	1.40e-11	67.0
MS.gene017373.t1	AT5G61560	40.580	69	40	1	36	104	745	812	1.40e-11	67.0

Find 73 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTTCTTTGTTGATTA+AGG	0.152854	5.2:+66036375	MS.gene017373:CDS
TGTCTTTGTGATAGCCAAAA+TGG	0.207784	5.2:+66036652	MS.gene017373:CDS
TGAAGGGATTGTTGAATCTT+TGG	0.251918	5.2:+66036426	MS.gene017373:CDS
TTGCTATTTGCACCCCATTT+TGG	0.252053	5.2:-66036666	None:intergenic
ATTCTTGTTGATGCTGAAAA+AGG	0.259858	5.2:+66036598	MS.gene017373:CDS
TTCATCAAGTCTAGAGTTAC+TGG	0.261782	5.2:-66035785	None:intergenic
TGAGAAGAACGTTGTTCTTT+TGG	0.273407	5.2:+66036219	MS.gene017373:CDS
TGAATTGGTGAGAAAGATAA+AGG	0.287993	5.2:+66036096	MS.gene017373:CDS
ATCAAATTCTTTGAGCTGTT+TGG	0.315007	5.2:+66036312	MS.gene017373:CDS
ATTGGAGAAGTTTGTTGAAT+TGG	0.316509	5.2:+66036552	MS.gene017373:CDS
AAAGAGTAGGAAAGTGATAT+TGG	0.342934	5.2:+66036534	MS.gene017373:CDS
GTAACTTCATAACGAGTTAC+TGG	0.352002	5.2:-66036016	None:intergenic
TTGGAGAAGTTTGTTGAATT+GGG	0.354092	5.2:+66036553	MS.gene017373:CDS
ATTCTTTGAGCTGTTTGGTT+TGG	0.357701	5.2:+66036317	MS.gene017373:CDS
GGAGTTTGTGAGAAAGCTTT+AGG	0.360911	5.2:+66036619	MS.gene017373:CDS
AAGTTACTGATACGTGTAAT+AGG	0.361648	5.2:+66036032	MS.gene017373:CDS
GTGTGTTGATTCATCATCAT+TGG	0.376962	5.2:+66036939	MS.gene017373:CDS
TGATTCTTTCTCACGTGGTT+TGG	0.380428	5.2:+66036195	MS.gene017373:CDS
GTTAAGGTTAATGTTGAGAT+TGG	0.382429	5.2:+66036457	MS.gene017373:CDS
TATCATGGACAAAAGGTAGT+AGG	0.385260	5.2:+66035669	MS.gene017373:CDS
ATCTTTGGTCAACATGGTTA+AGG	0.388977	5.2:+66036441	MS.gene017373:CDS
TAAATTAGAATGGCTTCTTA+AGG	0.391586	5.2:-66036972	None:intergenic
GAAGTTTGTTGAATTGGGTT+TGG	0.405985	5.2:+66036558	MS.gene017373:CDS
GTCTCTATCGTAGGTTATGC+CGG	0.413834	5.2:-66035828	None:intergenic
TGCTTCTCTTGTTCTTGCTA+AGG	0.419188	5.2:+66036168	MS.gene017373:CDS
GGTGAGAAAGATAAAGGGTT+GGG	0.424541	5.2:+66036102	MS.gene017373:CDS
ATGCCGGTTGAAAGTGTAAC+GGG	0.424846	5.2:-66035812	None:intergenic
ATTCTACGAAGGGAGTGATT+TGG	0.431522	5.2:-66035929	None:intergenic
TTCGTAGAATGATTCAAGAT+TGG	0.433178	5.2:+66035942	MS.gene017373:CDS
GGAAATGAATGTCGAGTCAT+TGG	0.449279	5.2:+66036396	MS.gene017373:CDS
GTCTTTGTGATAGCCAAAAT+GGG	0.454330	5.2:+66036653	MS.gene017373:CDS
TTGATTGAGGCAATTCAACT+TGG	0.459886	5.2:+66036808	MS.gene017373:CDS
AAGAAGGTTATAGTTTCAAA+TGG	0.465955	5.2:+66036145	MS.gene017373:CDS
GACCCCGTTACACTTTCAAC+CGG	0.468981	5.2:+66035809	MS.gene017373:CDS
GAATTGGTGAGAAAGATAAA+GGG	0.484864	5.2:+66036097	MS.gene017373:CDS
TTACTGGAATACGTGGAGTT+GGG	0.486770	5.2:-66036000	None:intergenic
GAGTCATTGGTGAAAATTGA+AGG	0.487284	5.2:+66036409	MS.gene017373:CDS
CAATTCAGTAGCTTTCTCCT+TGG	0.500670	5.2:-66036881	None:intergenic
ATCATGGACAAAAGGTAGTA+GGG	0.502085	5.2:+66035670	MS.gene017373:CDS
TGTGTTCAACACCGTTCCTA+TGG	0.506903	5.2:+66035965	MS.gene017373:CDS
TTCTTTCGACGCCATAGGAA+CGG	0.509833	5.2:-66035976	None:intergenic
TCCTCATAGATAAATTAGAA+TGG	0.519150	5.2:-66036982	None:intergenic
TACGTGTAATAGGATTCTTT+CGG	0.519461	5.2:+66036042	MS.gene017373:CDS
CAAGATTTCTGACAAGGCTG+AGG	0.519765	5.2:+66036777	MS.gene017373:CDS
GTTGGGGAAAAGAGAGTGAG+AGG	0.520190	5.2:+66036119	MS.gene017373:CDS
TTCGATGCTGTCTCTATCGT+AGG	0.522127	5.2:-66035837	None:intergenic
GTTACTGGAATACGTGGAGT+TGG	0.523278	5.2:-66036001	None:intergenic
TGGTTTACTCATCAAAGAGT+AGG	0.526893	5.2:+66036521	MS.gene017373:CDS
GAATTGTTGAAATTGTTGAA+TGG	0.533871	5.2:+66036898	MS.gene017373:CDS
TGTTGAATCTTTGGTCAACA+TGG	0.533879	5.2:+66036435	MS.gene017373:CDS
AAAAGCTTCTTAATAACAAG+AGG	0.544112	5.2:-66036709	None:intergenic
AGAGAGTGAGAGGAACAAGA+AGG	0.555137	5.2:+66036129	MS.gene017373:CDS
ATCTTGAATCATTCTACGAA+GGG	0.557256	5.2:-66035939	None:intergenic
TGATTGAGGCAATTCAACTT+GGG	0.559461	5.2:+66036809	MS.gene017373:CDS
TGGGATTCTTTCGACGCCAT+AGG	0.559846	5.2:-66035981	None:intergenic
TTCATGCAATGATTTACAAG+TGG	0.567554	5.2:+66035733	MS.gene017373:CDS
GTGAGAAAGATAAAGGGTTG+GGG	0.581859	5.2:+66036103	MS.gene017373:CDS
ACTGGGCAACGAAAATGAGT+TGG	0.587838	5.2:-66035767	None:intergenic
AGTCATTGGTGAAAATTGAA+GGG	0.590339	5.2:+66036410	MS.gene017373:CDS
ATTCTTTCGGCAGCGCAACA+AGG	0.598395	5.2:+66036055	MS.gene017373:CDS
TGTTTACAAGATTTCTGACA+AGG	0.599364	5.2:+66036771	MS.gene017373:CDS
GTGATTTGGTACGATGTCAA+AGG	0.600729	5.2:-66035915	None:intergenic
TCATCAAGTCTAGAGTTACT+GGG	0.601581	5.2:-66035784	None:intergenic
AATCTTGAATCATTCTACGA+AGG	0.617281	5.2:-66035940	None:intergenic
TGGTGAGAAAGATAAAGGGT+TGG	0.619284	5.2:+66036101	MS.gene017373:CDS
GATGCGTCCTTTGTCTGAAG+AGG	0.622117	5.2:+66036267	MS.gene017373:CDS
ATGCGTCCTTTGTCTGAAGA+GGG	0.633362	5.2:+66036268	MS.gene017373:CDS
AATCTACCCTCTTCAGACAA+AGG	0.640481	5.2:-66036274	None:intergenic
TATGCCGGTTGAAAGTGTAA+CGG	0.642906	5.2:-66035813	None:intergenic
TAACGAGTTACTGGAATACG+TGG	0.686345	5.2:-66036007	None:intergenic
TCTTTGTGATAGCCAAAATG+GGG	0.736673	5.2:+66036654	MS.gene017373:CDS
AGGTTGTGCTGAGAGTACCA+AGG	0.750134	5.2:+66036864	MS.gene017373:CDS
TGCCGGTTGAAAGTGTAACG+GGG	0.753225	5.2:-66035811	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCAACAAAAAATTTCAACT+AGG	+	chr5.2:66036350-66036369	MS.gene017373:CDS	20.0%
!!!	TTTGTCAACAATTTTTCATT+TGG	+	chr5.2:66036501-66036520	MS.gene017373:CDS	20.0%
!	AAAAGCTTCTTAATAACAAG+AGG	-	chr5.2:66036712-66036731	None:intergenic	25.0%
!	AAGAAGGTTATAGTTTCAAA+TGG	+	chr5.2:66036145-66036164	MS.gene017373:CDS	25.0%
!	GAATTGTTGAAATTGTTGAA+TGG	+	chr5.2:66036898-66036917	MS.gene017373:CDS	25.0%
!!	AAATGCTTCTTTGTTGATTA+AGG	+	chr5.2:66036375-66036394	MS.gene017373:CDS	25.0%
!!	TGTTATTAAGAAGCTTTTGA+GGG	+	chr5.2:66036714-66036733	MS.gene017373:CDS	25.0%
!!!	TGTTAACTCTGTTTTTGTAA+CGG	-	chr5.2:66035894-66035913	None:intergenic	25.0%
!!!	TTGTTATTAAGAAGCTTTTG+AGG	+	chr5.2:66036713-66036732	MS.gene017373:CDS	25.0%
	AAAGAGTAGGAAAGTGATAT+TGG	+	chr5.2:66036534-66036553	MS.gene017373:CDS	30.0%
	AAGTTACTGATACGTGTAAT+AGG	+	chr5.2:66036032-66036051	MS.gene017373:CDS	30.0%
	AATCTTGAATCATTCTACGA+AGG	-	chr5.2:66035943-66035962	None:intergenic	30.0%
	AGTCATTGGTGAAAATTGAA+GGG	+	chr5.2:66036410-66036429	MS.gene017373:CDS	30.0%
	ATCAAATTCTTTGAGCTGTT+TGG	+	chr5.2:66036312-66036331	MS.gene017373:CDS	30.0%
	ATCGAAAAATGGTTTGAATC+AGG	+	chr5.2:66035854-66035873	MS.gene017373:CDS	30.0%
	ATCTTGAATCATTCTACGAA+GGG	-	chr5.2:66035942-66035961	None:intergenic	30.0%
	ATTCTTGTTGATGCTGAAAA+AGG	+	chr5.2:66036598-66036617	MS.gene017373:CDS	30.0%
	GAATTGGTGAGAAAGATAAA+GGG	+	chr5.2:66036097-66036116	MS.gene017373:CDS	30.0%
	GTTAAGGTTAATGTTGAGAT+TGG	+	chr5.2:66036457-66036476	MS.gene017373:CDS	30.0%
	TACGTGTAATAGGATTCTTT+CGG	+	chr5.2:66036042-66036061	MS.gene017373:CDS	30.0%
	TGAATTGGTGAGAAAGATAA+AGG	+	chr5.2:66036096-66036115	MS.gene017373:CDS	30.0%
	TGTTTACAAGATTTCTGACA+AGG	+	chr5.2:66036771-66036790	MS.gene017373:CDS	30.0%
	TTCATGCAATGATTTACAAG+TGG	+	chr5.2:66035733-66035752	MS.gene017373:CDS	30.0%
	TTCGTAGAATGATTCAAGAT+TGG	+	chr5.2:66035942-66035961	MS.gene017373:CDS	30.0%
!!	ATTGGAGAAGTTTGTTGAAT+TGG	+	chr5.2:66036552-66036571	MS.gene017373:CDS	30.0%
!!	GGGTTTGGTTTCAATTTTAT+TGG	+	chr5.2:66036573-66036592	MS.gene017373:CDS	30.0%
!!	TGTTCTTTTGGAAGAGATTT+TGG	+	chr5.2:66036231-66036250	MS.gene017373:CDS	30.0%
!!	TTGGAGAAGTTTGTTGAATT+GGG	+	chr5.2:66036553-66036572	MS.gene017373:CDS	30.0%
!!!	ATGGTTTTATCATGGACAAA+AGG	+	chr5.2:66035662-66035681	MS.gene017373:CDS	30.0%
!!!	GTTAACTCTGTTTTTGTAAC+GGG	-	chr5.2:66035893-66035912	None:intergenic	30.0%
!!!	TGAGGAAAGTATTTTGATTG+AGG	+	chr5.2:66036795-66036814	MS.gene017373:CDS	30.0%
	ATCATGGACAAAAGGTAGTA+GGG	+	chr5.2:66035670-66035689	MS.gene017373:CDS	35.0%
	ATCTTTGGTCAACATGGTTA+AGG	+	chr5.2:66036441-66036460	MS.gene017373:CDS	35.0%
	ATTCTTTGAGCTGTTTGGTT+TGG	+	chr5.2:66036317-66036336	MS.gene017373:CDS	35.0%
	GAGTCATTGGTGAAAATTGA+AGG	+	chr5.2:66036409-66036428	MS.gene017373:CDS	35.0%
	GTAACTTCATAACGAGTTAC+TGG	-	chr5.2:66036019-66036038	None:intergenic	35.0%
	GTCTTTGTGATAGCCAAAAT+GGG	+	chr5.2:66036653-66036672	MS.gene017373:CDS	35.0%
	TATCATGGACAAAAGGTAGT+AGG	+	chr5.2:66035669-66035688	MS.gene017373:CDS	35.0%
	TCTTTGTGATAGCCAAAATG+GGG	+	chr5.2:66036654-66036673	MS.gene017373:CDS	35.0%
	TGAAGGGATTGTTGAATCTT+TGG	+	chr5.2:66036426-66036445	MS.gene017373:CDS	35.0%
	TGATTGAGGCAATTCAACTT+GGG	+	chr5.2:66036809-66036828	MS.gene017373:CDS	35.0%
	TGGTTTACTCATCAAAGAGT+AGG	+	chr5.2:66036521-66036540	MS.gene017373:CDS	35.0%
	TGTCTTTGTGATAGCCAAAA+TGG	+	chr5.2:66036652-66036671	MS.gene017373:CDS	35.0%
	TGTTGAATCTTTGGTCAACA+TGG	+	chr5.2:66036435-66036454	MS.gene017373:CDS	35.0%
	TTGATTGAGGCAATTCAACT+TGG	+	chr5.2:66036808-66036827	MS.gene017373:CDS	35.0%
!	AGAGGGTAGATTTGTCTTTT+TGG	+	chr5.2:66036285-66036304	MS.gene017373:CDS	35.0%
!	ATAGAGACAGCATCGAAAAA+TGG	+	chr5.2:66035843-66035862	MS.gene017373:CDS	35.0%
!	GAGGGTAGATTTGTCTTTTT+GGG	+	chr5.2:66036286-66036305	MS.gene017373:CDS	35.0%
!	TCATCAAGTCTAGAGTTACT+GGG	-	chr5.2:66035787-66035806	None:intergenic	35.0%
!	TGAGAAGAACGTTGTTCTTT+TGG	+	chr5.2:66036219-66036238	MS.gene017373:CDS	35.0%
!	TTCATCAAGTCTAGAGTTAC+TGG	-	chr5.2:66035788-66035807	None:intergenic	35.0%
!!	GAAGTTTGTTGAATTGGGTT+TGG	+	chr5.2:66036558-66036577	MS.gene017373:CDS	35.0%
!!	GTGTGTTGATTCATCATCAT+TGG	+	chr5.2:66036939-66036958	MS.gene017373:CDS	35.0%
!!	GTTTTTGATTCTTTCTCACG+TGG	+	chr5.2:66036190-66036209	MS.gene017373:CDS	35.0%
!!!	CTACTTGTTTTGTTGCAAGT+AGG	+	chr5.2:66036844-66036863	MS.gene017373:CDS	35.0%
	AATCTACCCTCTTCAGACAA+AGG	-	chr5.2:66036277-66036296	None:intergenic	40.0%
	ATTCTACGAAGGGAGTGATT+TGG	-	chr5.2:66035932-66035951	None:intergenic	40.0%
	CAATTCAGTAGCTTTCTCCT+TGG	-	chr5.2:66036884-66036903	None:intergenic	40.0%
	GGAAATGAATGTCGAGTCAT+TGG	+	chr5.2:66036396-66036415	MS.gene017373:CDS	40.0%
	GGAGTTTGTGAGAAAGCTTT+AGG	+	chr5.2:66036619-66036638	MS.gene017373:CDS	40.0%
	GGTGAGAAAGATAAAGGGTT+GGG	+	chr5.2:66036102-66036121	MS.gene017373:CDS	40.0%
	GTGAGAAAGATAAAGGGTTG+GGG	+	chr5.2:66036103-66036122	MS.gene017373:CDS	40.0%
	TAACGAGTTACTGGAATACG+TGG	-	chr5.2:66036010-66036029	None:intergenic	40.0%
	TGATTCTTTCTCACGTGGTT+TGG	+	chr5.2:66036195-66036214	MS.gene017373:CDS	40.0%
	TGCTTCTCTTGTTCTTGCTA+AGG	+	chr5.2:66036168-66036187	MS.gene017373:CDS	40.0%
	TGGTGAGAAAGATAAAGGGT+TGG	+	chr5.2:66036101-66036120	MS.gene017373:CDS	40.0%
	TTACTGGAATACGTGGAGTT+GGG	-	chr5.2:66036003-66036022	None:intergenic	40.0%
!	CTTTTCCTTTTCTTGCACGA+CGG	-	chr5.2:66035700-66035719	None:intergenic	40.0%
!	TATGCCGGTTGAAAGTGTAA+CGG	-	chr5.2:66035816-66035835	None:intergenic	40.0%
!!	CGAGAGCAAGTGTTTTGAAT+TGG	+	chr5.2:66036081-66036100	MS.gene017373:CDS	40.0%
!!	GTGATTTGGTACGATGTCAA+AGG	-	chr5.2:66035918-66035937	None:intergenic	40.0%
!!!	AGATTTTGGAAGTGCTGACA+TGG	+	chr5.2:66036245-66036264	MS.gene017373:CDS	40.0%
!!!	TTGCTATTTGCACCCCATTT+TGG	-	chr5.2:66036669-66036688	None:intergenic	40.0%
	ACTGGGCAACGAAAATGAGT+TGG	-	chr5.2:66035770-66035789	None:intergenic	45.0%
	AGAGAGTGAGAGGAACAAGA+AGG	+	chr5.2:66036129-66036148	MS.gene017373:CDS	45.0%
	ATGCGTCCTTTGTCTGAAGA+GGG	+	chr5.2:66036268-66036287	MS.gene017373:CDS	45.0%
	CAAGATTTCTGACAAGGCTG+AGG	+	chr5.2:66036777-66036796	MS.gene017373:CDS	45.0%
	GTCTCTATCGTAGGTTATGC+CGG	-	chr5.2:66035831-66035850	None:intergenic	45.0%
	GTTACTGGAATACGTGGAGT+TGG	-	chr5.2:66036004-66036023	None:intergenic	45.0%
	TGTGTTCAACACCGTTCCTA+TGG	+	chr5.2:66035965-66035984	MS.gene017373:CDS	45.0%
	TTCGATGCTGTCTCTATCGT+AGG	-	chr5.2:66035840-66035859	None:intergenic	45.0%
	TTCTTTCGACGCCATAGGAA+CGG	-	chr5.2:66035979-66035998	None:intergenic	45.0%
!	ATGCCGGTTGAAAGTGTAAC+GGG	-	chr5.2:66035815-66035834	None:intergenic	45.0%
!	GTTTTCCGTCGTGCAAGAAA+AGG	+	chr5.2:66035692-66035711	MS.gene017373:CDS	45.0%
	ATTCTTTCGGCAGCGCAACA+AGG	+	chr5.2:66036055-66036074	MS.gene017373:CDS	50.0%
	GACCCCGTTACACTTTCAAC+CGG	+	chr5.2:66035809-66035828	MS.gene017373:CDS	50.0%
	GATGCGTCCTTTGTCTGAAG+AGG	+	chr5.2:66036267-66036286	MS.gene017373:CDS	50.0%
	GTTGGGGAAAAGAGAGTGAG+AGG	+	chr5.2:66036119-66036138	MS.gene017373:CDS	50.0%
	TGGGATTCTTTCGACGCCAT+AGG	-	chr5.2:66035984-66036003	None:intergenic	50.0%
!	TGCCGGTTGAAAGTGTAACG+GGG	-	chr5.2:66035814-66035833	None:intergenic	50.0%
!!	AGGTTGTGCTGAGAGTACCA+AGG	+	chr5.2:66036864-66036883	MS.gene017373:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	66035662	66036990	66035662	ID=MS.gene017373
chr5.2	mRNA	66035662	66036990	66035662	ID=MS.gene017373.t1;Parent=MS.gene017373
chr5.2	exon	66035662	66036990	66035662	ID=MS.gene017373.t1.exon1;Parent=MS.gene017373.t1
chr5.2	CDS	66035662	66036990	66035662	ID=cds.MS.gene017373.t1;Parent=MS.gene017373.t1

Gene Sequence

>MS.gene017373

ATGGTTTTATCATGGACAAAAGGTAGTAGGGTTTTCCGTCGTGCAAGAAAAGGAAAAGAGTTAATATCAAGTTCATGCAATGATTTACAAGTGGAGATAGCAATTCCAACTCATTTTCGTTGCCCAGTAACTCTAGACTTGATGAAAGACCCCGTTACACTTTCAACCGGCATAACCTACGATAGAGACAGCATCGAAAAATGGTTTGAATCAGGTAACGACACATGTCCCGTTACAAAAACAGAGTTAACATCCTTTGACATCGTACCAAATCACTCCCTTCGTAGAATGATTCAAGATTGGTGTGTTCAACACCGTTCCTATGGCGTCGAAAGAATCCCAACTCCACGTATTCCAGTAACTCGTTATGAAGTTACTGATACGTGTAATAGGATTCTTTCGGCAGCGCAACAAGGAGACGAGAGCAAGTGTTTTGAATTGGTGAGAAAGATAAAGGGTTGGGGAAAAGAGAGTGAGAGGAACAAGAAGGTTATAGTTTCAAATGGTGCTTCTCTTGTTCTTGCTAAGGTTTTTGATTCTTTCTCACGTGGTTTGGTTGAGAAGAACGTTGTTCTTTTGGAAGAGATTTTGGAAGTGCTGACATGGATGCGTCCTTTGTCTGAAGAGGGTAGATTTGTCTTTTTGGGATCATCAAATTCTTTGAGCTGTTTGGTTTGGTTCTTAAATGATCAACAAAAAATTTCAACTAGGCAAAATGCTTCTTTGTTGATTAAGGAAATGAATGTCGAGTCATTGGTGAAAATTGAAGGGATTGTTGAATCTTTGGTCAACATGGTTAAGGTTAATGTTGAGATTGGAAGTGCTTCTACAAAAGCATGTTTGTCAACAATTTTTCATTTGGTTTACTCATCAAAGAGTAGGAAAGTGATATTGGAGAAGTTTGTTGAATTGGGTTTGGTTTCAATTTTATTGGAGATTCTTGTTGATGCTGAAAAAGGAGTTTGTGAGAAAGCTTTAGGTGTGTTGAATTGTCTTTGTGATAGCCAAAATGGGGTGCAAATAGCAAAATCAAATGCTTTGACTTTGCCTCTTGTTATTAAGAAGCTTTTGAGGGTTTCTGAATTGAGTTCTAGTTTTGTTGTTTCTATTGTTTACAAGATTTCTGACAAGGCTGAGGAAAGTATTTTGATTGAGGCAATTCAACTTGGGATGTTTCAAAAACTACTTGTTTTGTTGCAAGTAGGTTGTGCTGAGAGTACCAAGGAGAAAGCTACTGAATTGTTGAAATTGTTGAATGGTTATAAAAGCAAAGCAGAGTGTGTTGATTCATCATCATTGGATTTCACACACCTTAAGAAGCCATTCTAA

Protein sequence

>MS.gene017373.t1

MVLSWTKGSRVFRRARKGKELISSSCNDLQVEIAIPTHFRCPVTLDLMKDPVTLSTGITYDRDSIEKWFESGNDTCPVTKTELTSFDIVPNHSLRRMIQDWCVQHRSYGVERIPTPRIPVTRYEVTDTCNRILSAAQQGDESKCFELVRKIKGWGKESERNKKVIVSNGASLVLAKVFDSFSRGLVEKNVVLLEEILEVLTWMRPLSEEGRFVFLGSSNSLSCLVWFLNDQQKISTRQNASLLIKEMNVESLVKIEGIVESLVNMVKVNVEIGSASTKACLSTIFHLVYSSKSRKVILEKFVELGLVSILLEILVDAEKGVCEKALGVLNCLCDSQNGVQIAKSNALTLPLVIKKLLRVSELSSSFVVSIVYKISDKAEESILIEAIQLGMFQKLLVLLQVGCAESTKEKATELLKLLNGYKSKAECVDSSSLDFTHLKKPF