AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061543

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061543.t1 MTR_3g065080 97.549 204 5 0 129 332 236 439 5.16e-134 388
MS.gene061543.t1 MTR_3g065080 96.875 128 4 0 1 128 1 128 1.42e-88 272
MS.gene061543.t1 MTR_5g077510 68.932 206 60 3 129 331 235 439 1.90e-86 267
MS.gene061543.t1 MTR_5g077510 65.891 129 41 3 1 128 1 127 2.71e-55 186
MS.gene061543.t1 MTR_6g071340 46.047 215 100 6 130 331 204 415 3.81e-48 167
MS.gene061543.t1 MTR_6g071340 63.542 96 35 0 33 128 2 97 3.66e-42 151
MS.gene061543.t1 MTR_5g048050 46.047 215 100 6 130 331 224 435 2.73e-47 165
MS.gene061543.t1 MTR_5g048050 62.500 96 36 0 33 128 22 117 3.24e-41 149
MS.gene061543.t1 MTR_4g085720 60.000 90 36 0 34 123 12 101 2.39e-37 138
MS.gene061543.t1 MTR_4g085720 30.435 184 120 4 145 327 228 404 1.70e-13 71.2
MS.gene061543.t1 MTR_2g087350 57.000 100 39 2 31 126 1 100 1.03e-34 131
MS.gene061543.t1 MTR_2g087350 31.553 206 131 3 130 329 201 402 4.65e-15 75.9
MS.gene061543.t1 MTR_1g056840 59.794 97 33 2 32 122 3 99 1.33e-33 128
MS.gene061543.t1 MTR_7g106340 57.143 98 39 2 32 126 4 101 1.35e-33 128
MS.gene061543.t1 MTR_7g106340 34.615 208 123 5 130 329 215 417 6.37e-19 87.4
MS.gene061543.t1 MTR_1g056880 59.794 97 33 2 32 122 3 99 6.75e-33 127
MS.gene061543.t1 MTR_1g056880 33.333 210 128 5 127 329 229 433 1.05e-17 84.0
MS.gene061543.t1 MTR_1g056910 39.196 199 79 7 31 221 1 165 8.30e-33 126
MS.gene061543.t1 MTR_1g056870 57.732 97 35 2 32 122 3 99 1.44e-31 123
MS.gene061543.t1 MTR_1g056870 33.333 210 128 5 127 329 229 433 1.46e-17 83.6
MS.gene061543.t1 MTR_3g096370 68.571 70 22 0 33 102 7 76 8.95e-28 112
MS.gene061543.t1 MTR_8g092870 41.406 128 65 3 34 161 8 125 9.02e-26 107
MS.gene061543.t1 MTR_3g095730 47.674 86 44 1 34 119 11 95 1.02e-23 101
MS.gene061543.t1 MTR_7g059405 51.190 84 36 2 36 119 264 342 3.76e-23 100
MS.gene061543.t1 MTR_4g028960 33.690 187 104 5 22 204 262 432 5.32e-23 100
MS.gene061543.t1 MTR_5g083030 43.299 97 55 0 34 130 291 387 8.21e-23 99.8
MS.gene061543.t1 MTR_4g485520 48.684 76 39 0 32 107 101 176 9.83e-23 99.0
MS.gene061543.t1 MTR_4g063800 30.743 296 176 10 25 318 244 512 1.35e-22 99.0
MS.gene061543.t1 MTR_4g485520 48.684 76 39 0 32 107 269 344 1.85e-22 98.6
MS.gene061543.t1 MTR_4g107010 42.308 104 60 0 3 106 247 350 1.92e-22 98.6
MS.gene061543.t1 MTR_3g085610 43.956 91 45 1 37 127 281 365 7.89e-22 96.7
MS.gene061543.t1 MTR_7g078330 30.315 254 141 9 9 234 240 485 1.49e-21 95.9
MS.gene061543.t1 MTR_2g018010 48.780 82 39 1 34 115 290 368 2.28e-21 95.5
MS.gene061543.t1 MTR_4g051515 46.809 94 46 3 33 122 3 96 1.01e-20 92.4
MS.gene061543.t1 MTR_4g051515 26.415 159 112 2 165 322 250 404 4.23e-12 67.0
MS.gene061543.t1 MTR_7g053260 52.174 69 33 0 34 102 76 144 1.26e-20 92.4
MS.gene061543.t1 MTR_3g078160 44.792 96 47 2 37 130 276 367 1.75e-20 92.8
MS.gene061543.t1 MTR_3g078160 44.792 96 47 2 37 130 276 367 2.01e-20 92.4
MS.gene061543.t1 MTR_7g116600 41.441 111 61 2 32 142 74 180 3.56e-20 91.3
MS.gene061543.t1 MTR_3g078160 52.857 70 33 0 37 106 276 345 4.75e-20 91.3
MS.gene061543.t1 MTR_5g034440 55.224 67 30 0 40 106 284 350 7.21e-20 90.9
MS.gene061543.t1 MTR_1g069845 47.143 70 37 0 34 103 122 191 2.55e-19 89.4
MS.gene061543.t1 MTR_1g069845 47.143 70 37 0 34 103 243 312 4.40e-19 88.6
MS.gene061543.t1 MTR_1g069845 47.143 70 37 0 34 103 239 308 4.47e-19 88.6
MS.gene061543.t1 MTR_7g097020 44.595 74 41 0 34 107 239 312 4.72e-19 88.6
MS.gene061543.t1 MTR_8g080280 39.560 91 49 1 37 127 283 367 1.50e-18 87.0
MS.gene061543.t1 MTR_4g091880 47.826 69 35 1 35 102 4 72 2.00e-18 85.1
MS.gene061543.t1 MTR_4g091880 47.826 69 35 1 35 102 4 72 2.08e-18 85.5
MS.gene061543.t1 MTR_1g094215 53.521 71 33 0 36 106 247 317 8.08e-18 84.7
MS.gene061543.t1 MTR_1g094215 53.521 71 33 0 36 106 64 134 8.35e-18 84.3
MS.gene061543.t1 MTR_1g017770 48.148 81 38 1 26 102 31 111 1.14e-17 83.6
MS.gene061543.t1 MTR_1g079450 35.537 121 74 2 34 154 61 177 1.86e-17 83.2
MS.gene061543.t1 MTR_5g015210 25.843 267 182 7 36 292 67 327 1.11e-16 80.9
MS.gene061543.t1 MTR_8g077205 38.614 101 56 2 17 117 146 240 1.48e-16 80.9
MS.gene061543.t1 MTR_8g077205 38.614 101 56 2 17 117 258 352 1.53e-16 80.9
MS.gene061543.t1 MTR_1g093965 27.919 197 127 3 49 234 1 193 1.68e-16 77.0
MS.gene061543.t1 MTR_2g011140 41.463 82 47 1 21 102 1 81 2.74e-16 79.3
MS.gene061543.t1 MTR_3g466220 41.892 74 43 0 34 107 231 304 5.81e-16 79.3
MS.gene061543.t1 MTR_1g093995 27.919 197 127 3 49 234 1 193 5.94e-16 75.5
MS.gene061543.t1 MTR_3g466220 41.892 74 43 0 34 107 210 283 6.02e-16 79.3
MS.gene061543.t1 MTR_5g020570 38.372 86 49 2 32 117 269 350 4.41e-15 76.6
MS.gene061543.t1 MTR_2g096850 45.070 71 39 0 32 102 585 655 4.85e-15 76.3
MS.gene061543.t1 MTR_5g020570 38.372 86 49 2 32 117 284 365 4.87e-15 76.6
MS.gene061543.t1 MTR_2g096850 45.070 71 39 0 32 102 736 806 5.67e-15 76.3
MS.gene061543.t1 MTR_3g096370 72.093 43 12 0 33 75 7 49 2.13e-14 73.2
MS.gene061543.t1 MTR_1g090320 40.625 96 48 4 31 118 504 598 2.34e-13 71.6
MS.gene061543.t1 MTR_1g090320 40.625 96 48 4 31 118 506 600 2.40e-13 71.6
MS.gene061543.t1 MTR_7g117890 38.750 80 49 0 40 119 259 338 3.23e-13 70.9
MS.gene061543.t1 MTR_8g103227 38.636 88 48 2 22 108 386 468 6.38e-13 70.1
MS.gene061543.t1 MTR_5g032010 34.247 73 48 0 36 108 732 804 2.04e-12 68.6
MS.gene061543.t1 MTR_3g008270 43.939 66 36 1 37 102 731 795 4.12e-12 67.4
MS.gene061543.t1 MTR_1g100820 36.364 66 42 0 37 102 649 714 6.11e-12 67.0
MS.gene061543.t1 MTR_1g100820 36.364 66 42 0 37 102 647 712 6.16e-12 67.0
MS.gene061543.t1 MTR_4g125930 39.130 69 41 1 37 105 492 559 9.97e-12 66.2
MS.gene061543.t1 MTR_4g125930 39.130 69 41 1 37 105 692 759 1.27e-11 65.9
MS.gene061543.t1 MTR_7g077780 36.364 66 42 0 37 102 827 892 1.33e-11 66.2
MS.gene061543.t1 MTR_3g115670 36.000 100 55 5 22 112 12 111 1.34e-11 65.9
MS.gene061543.t1 MTR_8g027140 40.278 72 43 0 40 111 263 334 2.89e-11 65.1
MS.gene061543.t1 MTR_3g008280 43.939 66 36 1 37 102 732 796 4.99e-11 64.3
MS.gene061543.t1 MTR_3g008280 43.939 66 36 1 37 102 740 804 5.23e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061543.t1 AT3G02840 35.659 387 182 14 1 331 1 376 1.16e-55 186
MS.gene061543.t1 AT5G37490 68.000 100 32 0 33 132 28 127 1.45e-42 152
MS.gene061543.t1 AT5G37490 41.315 213 112 5 127 331 225 432 3.36e-41 149
MS.gene061543.t1 AT1G66160 61.458 96 37 0 33 128 30 125 4.66e-39 143
MS.gene061543.t1 AT1G66160 36.667 210 114 5 132 331 228 428 6.13e-33 127
MS.gene061543.t1 AT2G35930 53.125 128 57 2 27 153 3 128 9.65e-39 142
MS.gene061543.t1 AT2G35930 32.536 209 128 3 130 329 205 409 1.48e-18 86.3
MS.gene061543.t1 AT1G49780 50.400 125 62 0 34 158 12 136 4.73e-38 140
MS.gene061543.t1 AT3G52450 48.951 143 60 4 32 173 3 133 1.10e-37 139
MS.gene061543.t1 AT3G52450 33.146 178 111 2 156 329 260 433 5.96e-17 81.6
MS.gene061543.t1 AT3G19380 50.407 123 56 2 34 151 12 134 7.29e-35 132
MS.gene061543.t1 AT3G19380 32.051 156 98 5 146 299 235 384 2.29e-11 64.7
MS.gene061543.t1 AT1G66160 36.667 210 114 5 132 331 212 412 6.68e-33 126
MS.gene061543.t1 AT1G66160 51.042 96 31 1 33 128 30 109 9.78e-28 112
MS.gene061543.t1 AT3G11840 49.167 120 54 3 32 150 6 119 3.55e-28 114
MS.gene061543.t1 AT3G11840 33.333 165 104 2 168 330 287 447 1.15e-12 68.9
MS.gene061543.t1 AT3G46510 31.058 293 157 11 36 318 256 513 5.06e-25 106
MS.gene061543.t1 AT1G23030 53.947 76 35 0 32 107 237 312 2.93e-24 103
MS.gene061543.t1 AT1G71020 27.622 286 181 6 26 310 85 345 2.42e-23 100
MS.gene061543.t1 AT5G64660 50.000 82 33 1 34 107 8 89 2.88e-23 99.8
MS.gene061543.t1 AT1G71020 27.622 286 181 6 26 310 233 493 3.42e-23 100
MS.gene061543.t1 AT3G18710 40.496 121 60 2 34 149 10 123 2.51e-22 97.1
MS.gene061543.t1 AT5G09800 33.557 149 89 2 35 173 10 158 7.16e-22 95.9
MS.gene061543.t1 AT3G54850 53.247 77 33 1 36 112 248 321 1.08e-21 96.3
MS.gene061543.t1 AT3G07360 50.000 76 38 0 31 106 69 144 3.81e-21 94.0
MS.gene061543.t1 AT2G28830 26.603 312 189 10 16 318 166 446 9.64e-21 93.6
MS.gene061543.t1 AT1G29340 50.588 85 41 1 34 117 303 387 1.05e-20 93.6
MS.gene061543.t1 AT1G27910 41.758 91 47 1 37 127 280 364 1.85e-19 89.7
MS.gene061543.t1 AT5G01830 45.946 74 40 0 30 103 268 341 3.58e-19 89.0
MS.gene061543.t1 AT5G42340 27.468 233 136 5 34 244 288 509 1.04e-18 87.4
MS.gene061543.t1 AT3G54790 49.315 73 37 0 34 106 200 272 1.17e-18 87.4
MS.gene061543.t1 AT3G54790 49.315 73 37 0 34 106 236 308 1.45e-18 87.0
MS.gene061543.t1 AT3G54790 49.315 73 37 0 34 106 236 308 1.45e-18 87.0
MS.gene061543.t1 AT3G49810 29.670 182 118 3 36 209 64 243 1.49e-18 86.7
MS.gene061543.t1 AT4G21350 50.000 68 33 1 36 102 5 72 1.51e-18 85.9
MS.gene061543.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.56e-18 86.7
MS.gene061543.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.58e-18 87.0
MS.gene061543.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.58e-18 87.0
MS.gene061543.t1 AT1G67530 45.000 80 40 1 37 116 273 348 1.58e-18 87.0
MS.gene061543.t1 AT1G24330 40.659 91 48 1 37 127 276 360 1.76e-18 87.0
MS.gene061543.t1 AT1G24330 40.659 91 48 1 37 127 276 360 1.76e-18 87.0
MS.gene061543.t1 AT5G67340 44.737 76 42 0 34 109 238 313 1.31e-17 84.3
MS.gene061543.t1 AT5G67340 44.737 76 42 0 34 109 238 313 1.31e-17 84.3
MS.gene061543.t1 AT5G65920 50.746 67 33 0 36 102 60 126 6.43e-16 78.6
MS.gene061543.t1 AT5G65920 50.746 67 33 0 36 102 60 126 6.43e-16 78.6
MS.gene061543.t1 AT4G36550 40.278 72 43 0 35 106 218 289 6.68e-16 79.0
MS.gene061543.t1 AT1G01660 35.556 90 57 1 18 107 481 569 2.87e-15 77.0
MS.gene061543.t1 AT2G23140 37.234 94 55 1 34 127 228 317 3.01e-15 77.0
MS.gene061543.t1 AT2G23140 37.234 94 55 1 34 127 228 317 3.01e-15 77.0
MS.gene061543.t1 AT2G23140 37.234 94 55 1 34 127 231 320 3.01e-15 77.0
MS.gene061543.t1 AT1G01660 35.556 90 57 1 18 107 480 568 3.03e-15 77.0
MS.gene061543.t1 AT2G45920 41.096 73 43 0 37 109 326 398 4.42e-15 75.9
MS.gene061543.t1 AT2G23140 37.234 94 55 1 34 127 39 128 4.77e-15 76.3
MS.gene061543.t1 AT2G23140 37.234 94 55 1 34 127 39 128 4.77e-15 76.3
MS.gene061543.t1 AT1G10560 47.692 65 34 0 42 106 294 358 4.90e-15 76.6
MS.gene061543.t1 AT1G01680 38.571 70 43 0 40 109 237 306 6.00e-15 74.7
MS.gene061543.t1 AT1G60190 46.269 67 36 0 40 106 282 348 7.01e-15 75.9
MS.gene061543.t1 AT5G57035 40.909 66 39 0 37 102 709 774 3.75e-14 73.9
MS.gene061543.t1 AT5G51270 44.286 70 38 1 37 106 736 804 1.78e-13 71.6
MS.gene061543.t1 AT5G51270 44.286 70 38 1 37 106 736 804 1.78e-13 71.6
MS.gene061543.t1 AT2G19410 43.939 66 36 1 37 102 712 776 2.36e-13 71.2
MS.gene061543.t1 AT1G56030 42.857 70 40 0 31 100 318 387 2.47e-13 70.9
MS.gene061543.t1 AT2G19410 43.939 66 36 1 37 102 726 790 2.60e-13 71.2
MS.gene061543.t1 AT2G19410 43.939 66 36 1 37 102 747 811 2.67e-13 71.2
MS.gene061543.t1 AT5G65500 40.299 67 40 0 36 102 723 789 2.72e-13 71.2
MS.gene061543.t1 AT5G65500 40.299 67 40 0 36 102 715 781 2.89e-13 71.2
MS.gene061543.t1 AT3G61390 37.879 66 41 0 37 102 279 344 1.79e-12 67.8
MS.gene061543.t1 AT3G49060 37.879 66 41 0 37 102 703 768 1.98e-12 68.6
MS.gene061543.t1 AT3G49060 37.879 66 41 0 37 102 726 791 2.00e-12 68.6
MS.gene061543.t1 AT3G49060 37.879 66 41 0 37 102 736 801 2.08e-12 68.6
MS.gene061543.t1 AT3G61390 37.879 66 41 0 37 102 354 419 2.28e-12 67.8
MS.gene061543.t1 AT1G56040 40.625 64 38 0 37 100 354 417 4.52e-12 67.0
MS.gene061543.t1 AT4G25160 40.909 66 38 1 37 102 767 831 2.67e-11 65.1
MS.gene061543.t1 AT5G18340 24.199 281 172 11 34 283 72 342 3.26e-11 64.3
MS.gene061543.t1 AT2G45910 37.879 66 41 0 37 102 764 829 3.50e-11 64.7
MS.gene061543.t1 AT2G45910 37.879 66 41 0 37 102 764 829 3.50e-11 64.7

Find 61 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATTGTTGATGGAGAAAA+AGG 0.166972 3.2:-43858478 MS.gene061543:CDS
GAAAGTTTCTGATTTGTAAC+TGG 0.278770 3.2:+43859181 None:intergenic
TAGTGAAAAGTTTGTTGAAT+TGG 0.291875 3.2:-43858524 MS.gene061543:CDS
ATTCCTGTTGATAGAGTTAC+TGG 0.298592 3.2:+43859259 None:intergenic
AGTGAAAAGTTTGTTGAATT+GGG 0.337948 3.2:-43858523 MS.gene061543:CDS
TTGCTTGAAACAATTGTTGA+TGG 0.366375 3.2:-43858487 MS.gene061543:CDS
TGTGTTGAAAATAGTTCTTA+TGG 0.375679 3.2:-43859106 MS.gene061543:CDS
TGAAGGTGTTGTTGAGGGTT+TGG 0.377823 3.2:-43858644 MS.gene061543:CDS
AAGATTGTTAAGGAACCAAT+TGG 0.381968 3.2:-43858619 MS.gene061543:CDS
GACTCACCGGACAACGGAAA+TGG 0.385326 3.2:+43859299 None:intergenic
GAAGATGCTTTGTGAGAAGA+AGG 0.393306 3.2:-43858299 MS.gene061543:CDS
TAATTCGCCGTCGCCGGCGC+CGG 0.395081 3.2:+43859348 None:intergenic
CGCCGGCGACGGCGAATTAA+CGG 0.401684 3.2:-43859344 MS.gene061543:CDS
TTTGTAGCACTTGACCCAAT+TGG 0.406484 3.2:+43858604 None:intergenic
CCCAACCCATTTCCGTTGTC+CGG 0.411042 3.2:-43859305 MS.gene061543:CDS
CACCGTTAATTCGCCGTCGC+CGG 0.412333 3.2:+43859342 None:intergenic
TGTTTATGTGATTGTAAGAA+AGG 0.414472 3.2:-43858424 MS.gene061543:CDS
TGTTGGTTATGTTGCAAGTT+GGG 0.414797 3.2:-43858228 MS.gene061543:CDS
AAAGCAAAACACCAACTTCC+CGG 0.416680 3.2:-43859367 MS.gene061543:CDS
AATTCGCCGTCGCCGGCGCC+GGG 0.439130 3.2:+43859349 None:intergenic
GTGTTGAAAATAGTTCTTAT+GGG 0.440410 3.2:-43859105 MS.gene061543:CDS
TGAAAAGGCACTAGGTGTAT+TGG 0.441241 3.2:-43858449 MS.gene061543:CDS
TTAACCTCGTACGCTGAAAC+AGG 0.447425 3.2:+43859055 None:intergenic
CGTCGCCGGCGCCGGGAAGT+TGG 0.449959 3.2:+43859356 None:intergenic
CCGGACAACGGAAATGGGTT+GGG 0.459441 3.2:+43859305 None:intergenic
GATCCAGTAACTCTATCAAC+AGG 0.465887 3.2:-43859262 MS.gene061543:CDS
GGGTTTGGTGAAGATTGTTA+AGG 0.467901 3.2:-43858629 MS.gene061543:CDS
AGAATTGAAGGTGTTGTTGA+GGG 0.472391 3.2:-43858649 MS.gene061543:CDS
ATAGATTCAGCATCGAGAAA+TGG 0.477438 3.2:-43859228 MS.gene061543:CDS
ACTCACCGGACAACGGAAAT+GGG 0.483936 3.2:+43859300 None:intergenic
GAAGAGTTATCAAGAATTGA+AGG 0.485697 3.2:-43858661 MS.gene061543:CDS
ACCGGACAACGGAAATGGGT+TGG 0.495073 3.2:+43859304 None:intergenic
GTTTATGTGATTGTAAGAAA+GGG 0.496421 3.2:-43858423 MS.gene061543:CDS
ATCGAGAAATGGATCGAAGC+TGG 0.513343 3.2:-43859217 MS.gene061543:CDS
TTGTTGGTTATGTTGCAAGT+TGG 0.518220 3.2:-43858229 MS.gene061543:CDS
CAACTTCCCGGCGCCGGCGA+CGG 0.518938 3.2:-43859355 MS.gene061543:CDS
AAAGTTTCTGATTTGTAACT+GGG 0.529983 3.2:+43859182 None:intergenic
AGAAACATGTTCCGCCGCAC+CGG 0.531078 3.2:-43859391 MS.gene061543:CDS
AAAAGCTTCTTAATCACAAG+AGG 0.544112 3.2:+43858367 None:intergenic
GAGTTGTTGAAATTGTTGAA+TGG 0.546361 3.2:-43858175 MS.gene061543:CDS
AAACACCAACTTCCCGGCGC+CGG 0.546949 3.2:-43859361 MS.gene061543:CDS
TTGATTGAAGCAATTCAACT+TGG 0.549684 3.2:-43858265 MS.gene061543:CDS
AAGAATTGAAGGTGTTGTTG+AGG 0.552708 3.2:-43858650 MS.gene061543:CDS
TTCAGCGTACGAGGTTAATG+AGG 0.552963 3.2:-43859050 MS.gene061543:intron
GAAACAGGTATACGAGGTGT+TGG 0.554990 3.2:+43859070 None:intergenic
GTGATTTGGTGTGATTTCGA+AGG 0.579587 3.2:+43859156 None:intergenic
TTCATCAAGTCTAGACTCAC+CGG 0.580014 3.2:+43859286 None:intergenic
AGATTGTTAAGGAACCAATT+GGG 0.580879 3.2:-43858618 MS.gene061543:CDS
CGACGGCGAATTAACGGTGG+AGG 0.584194 3.2:-43859338 MS.gene061543:CDS
TATGTTGCAAGTTGGGTGTG+AGG 0.588109 3.2:-43858221 MS.gene061543:CDS
AAGGGAAAGAGGTTGTTCAA+AGG 0.593560 3.2:-43858405 MS.gene061543:CDS
TGATTGTAAGAAAGGGAAAG+AGG 0.594131 3.2:-43858416 MS.gene061543:CDS
TATACCTGTTTCAGCGTACG+AGG 0.604860 3.2:-43859059 MS.gene061543:CDS
CTCGATGCTGAATCTATCGT+AGG 0.614355 3.2:+43859234 None:intergenic
GGGATATGTGAAAAGGCACT+AGG 0.622666 3.2:-43858457 MS.gene061543:CDS
ACAGCAAAACTAGAAGCCAA+AGG 0.636688 3.2:+43858334 None:intergenic
AGTCTAGACTCACCGGACAA+CGG 0.656680 3.2:+43859293 None:intergenic
TGGGTGTGAGGAGAAAACAA+AGG 0.673609 3.2:-43858209 MS.gene061543:CDS
GTGTGAGGAGAAAACAAAGG+AGG 0.681227 3.2:-43858206 MS.gene061543:CDS
TACGCTGAAACAGGTATACG+AGG 0.716974 3.2:+43859064 None:intergenic
CGGCGACGGCGAATTAACGG+TGG 0.722796 3.2:-43859341 MS.gene061543:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGATGGTAAAGATTTA+GGG - chr3.2:43858799-43858818 MS.gene061543:intron 20.0%
!!! TTTTTAGATGGTAAAGATTT+AGG - chr3.2:43858798-43858817 MS.gene061543:intron 20.0%
! CATCTTTGAATTGTTTAGTT+TGG - chr3.2:43858775-43858794 MS.gene061543:intron 25.0%
! GTGTTGAAAATAGTTCTTAT+GGG - chr3.2:43858421-43858440 MS.gene061543:CDS 25.0%
! GTTTATGTGATTGTAAGAAA+GGG - chr3.2:43859103-43859122 MS.gene061543:CDS 25.0%
! TAAGAAAAATGATTCAGAGT+TGG - chr3.2:43858397-43858416 MS.gene061543:CDS 25.0%
! TGTGTTGAAAATAGTTCTTA+TGG - chr3.2:43858420-43858439 MS.gene061543:CDS 25.0%
! TGTTTATGTGATTGTAAGAA+AGG - chr3.2:43859102-43859121 MS.gene061543:CDS 25.0%
!! AAAGTTTCTGATTTGTAACT+GGG + chr3.2:43858347-43858366 None:intergenic 25.0%
!! ATTTTTCTTATGGTGTGATT+TGG + chr3.2:43858387-43858406 None:intergenic 25.0%
!! TTTTTCATGTGTTTCTTTTG+AGG - chr3.2:43858656-43858675 MS.gene061543:CDS 25.0%
!!! AATTTTCAAGTTGGTTTCAT+CGG - chr3.2:43858960-43858979 MS.gene061543:intron 25.0%
!!! ACTCTGAATCATTTTTCTTA+TGG + chr3.2:43858397-43858416 None:intergenic 25.0%
!!! AGATTTTGGAGATTTTAACA+TGG - chr3.2:43858700-43858719 MS.gene061543:CDS 25.0%
!!! AGTGAAAAGTTTGTTGAATT+GGG - chr3.2:43859003-43859022 MS.gene061543:intron 25.0%
!!! TAGTGAAAAGTTTGTTGAAT+TGG - chr3.2:43859002-43859021 MS.gene061543:intron 25.0%
!!! TGTTTAGTTTGGTTTTTAGA+TGG - chr3.2:43858786-43858805 MS.gene061543:intron 25.0%
!!! TTTGACTACAATTTTCAAGT+TGG - chr3.2:43858951-43858970 MS.gene061543:intron 25.0%
AAAAGCTTCTTAATCACAAG+AGG + chr3.2:43859162-43859181 None:intergenic 30.0%
AAGAGGGTTATAATAGGAAA+TGG - chr3.2:43858600-43858619 MS.gene061543:CDS 30.0%
AAGATTGTTAAGGAACCAAT+TGG - chr3.2:43858907-43858926 MS.gene061543:intron 30.0%
AGAAAAAGGGATATGTGAAA+AGG - chr3.2:43859062-43859081 MS.gene061543:CDS 30.0%
AGATTGTTAAGGAACCAATT+GGG - chr3.2:43858908-43858927 MS.gene061543:intron 30.0%
AGGAATAAGAGGGTTATAAT+AGG - chr3.2:43858594-43858613 MS.gene061543:CDS 30.0%
GAAGAGTTATCAAGAATTGA+AGG - chr3.2:43858865-43858884 MS.gene061543:intron 30.0%
GAGTTGTTGAAATTGTTGAA+TGG - chr3.2:43859351-43859370 MS.gene061543:CDS 30.0%
TTGATTGAAGCAATTCAACT+TGG - chr3.2:43859261-43859280 MS.gene061543:CDS 30.0%
TTGCTTGAAACAATTGTTGA+TGG - chr3.2:43859039-43859058 MS.gene061543:intron 30.0%
TTGTTGGAAAGATTAAGGTT+TGG - chr3.2:43858556-43858575 MS.gene061543:CDS 30.0%
! AAAAAGTGTGTTGAGTTTGT+TGG - chr3.2:43858540-43858559 MS.gene061543:CDS 30.0%
! ACAATTGTTGATGGAGAAAA+AGG - chr3.2:43859048-43859067 MS.gene061543:intron 30.0%
! CAATTGTTGATGGAGAAAAA+GGG - chr3.2:43859049-43859068 MS.gene061543:intron 30.0%
! GAAAGTTTCTGATTTGTAAC+TGG + chr3.2:43858348-43858367 None:intergenic 30.0%
! TGTGATTAAGAAGCTTTTGA+GGG - chr3.2:43859164-43859183 MS.gene061543:CDS 30.0%
! TTGTGATTAAGAAGCTTTTG+AGG - chr3.2:43859163-43859182 MS.gene061543:CDS 30.0%
!! TGAGTTTGTTGGAAAGATTA+AGG - chr3.2:43858551-43858570 MS.gene061543:CDS 30.0%
!!! AGTTTGTTCTGTTTTAGCTA+CGG - chr3.2:43858623-43858642 MS.gene061543:CDS 30.0%
AGGTTAATGAGGTTTGTACT+AGG - chr3.2:43858487-43858506 MS.gene061543:CDS 35.0%
TGATTGTAAGAAAGGGAAAG+AGG - chr3.2:43859110-43859129 MS.gene061543:CDS 35.0%
TTGGAAAGATTAAGGTTTGG+TGG - chr3.2:43858559-43858578 MS.gene061543:CDS 35.0%
! ATAGATTCAGCATCGAGAAA+TGG - chr3.2:43858298-43858317 MS.gene061543:CDS 35.0%
! ATTCCTGTTGATAGAGTTAC+TGG + chr3.2:43858270-43858289 None:intergenic 35.0%
! TGGTGCTTTTCAGAAATTGT+TGG - chr3.2:43859281-43859300 MS.gene061543:CDS 35.0%
! TGTTGGTTATGTTGCAAGTT+GGG - chr3.2:43859298-43859317 MS.gene061543:CDS 35.0%
! TTGTTGGTTATGTTGCAAGT+TGG - chr3.2:43859297-43859316 MS.gene061543:CDS 35.0%
!! AAGAATTGAAGGTGTTGTTG+AGG - chr3.2:43858876-43858895 MS.gene061543:intron 35.0%
!! AGAATTGAAGGTGTTGTTGA+GGG - chr3.2:43858877-43858896 MS.gene061543:intron 35.0%
!!! TGAGGAACATGTTGTTGTTT+TGG - chr3.2:43858674-43858693 MS.gene061543:CDS 35.0%
!!! TGTTGTTTTGGAGGAGATTT+TGG - chr3.2:43858686-43858705 MS.gene061543:CDS 35.0%
AAAGCAAAACACCAACTTCC+CGG - chr3.2:43858159-43858178 MS.gene061543:CDS 40.0%
AAGGGAAAGAGGTTGTTCAA+AGG - chr3.2:43859121-43859140 MS.gene061543:CDS 40.0%
ACAGCAAAACTAGAAGCCAA+AGG + chr3.2:43859195-43859214 None:intergenic 40.0%
AGAGAGTGAGAGGAATAAGA+GGG - chr3.2:43858584-43858603 MS.gene061543:CDS 40.0%
GAAGATGCTTTGTGAGAAGA+AGG - chr3.2:43859227-43859246 MS.gene061543:CDS 40.0%
GATCCAGTAACTCTATCAAC+AGG - chr3.2:43858264-43858283 MS.gene061543:CDS 40.0%
GGTTTGTACTAGGTTGTTGT+CGG - chr3.2:43858497-43858516 MS.gene061543:CDS 40.0%
GTTTGTACTAGGTTGTTGTC+GGG - chr3.2:43858498-43858517 MS.gene061543:CDS 40.0%
TTCATCAAGTCTAGACTCAC+CGG + chr3.2:43858243-43858262 None:intergenic 40.0%
TTTGTAGCACTTGACCCAAT+TGG + chr3.2:43858925-43858944 None:intergenic 40.0%
! CGTTTTCGAATCGCTAAAAG+AGG + chr3.2:43858736-43858755 None:intergenic 40.0%
! GTTGTCGGGTTGTAGAAATT+TGG - chr3.2:43858512-43858531 MS.gene061543:CDS 40.0%
!! GGGTTTGGTGAAGATTGTTA+AGG - chr3.2:43858897-43858916 MS.gene061543:intron 40.0%
!! GTGATTTGGTGTGATTTCGA+AGG + chr3.2:43858373-43858392 None:intergenic 40.0%
!! TGAAAAGGCACTAGGTGTAT+TGG - chr3.2:43859077-43859096 MS.gene061543:CDS 40.0%
!!! AAGTTGGTGTTTTGCTTTGC+CGG + chr3.2:43858157-43858176 None:intergenic 40.0%
!!! GGAACATGTTGTTGTTTTGG+AGG - chr3.2:43858677-43858696 MS.gene061543:CDS 40.0%
!!! GTTTTGCTGTTGGGATTGTT+AGG - chr3.2:43859205-43859224 MS.gene061543:CDS 40.0%
!!! TGGCTTCTAGTTTTGCTGTT+GGG - chr3.2:43859196-43859215 MS.gene061543:CDS 40.0%
!!! TTGGCTTCTAGTTTTGCTGT+TGG - chr3.2:43859195-43859214 MS.gene061543:CDS 40.0%
ATCGAGAAATGGATCGAAGC+TGG - chr3.2:43858309-43858328 MS.gene061543:CDS 45.0%
CTCGATGCTGAATCTATCGT+AGG + chr3.2:43858295-43858314 None:intergenic 45.0%
GAAACAGGTATACGAGGTGT+TGG + chr3.2:43858459-43858478 None:intergenic 45.0%
GAGAGAGTGAGAGGAATAAG+AGG - chr3.2:43858583-43858602 MS.gene061543:CDS 45.0%
GTGTGAGGAGAAAACAAAGG+AGG - chr3.2:43859320-43859339 MS.gene061543:CDS 45.0%
TACGCTGAAACAGGTATACG+AGG + chr3.2:43858465-43858484 None:intergenic 45.0%
TATACCTGTTTCAGCGTACG+AGG - chr3.2:43858467-43858486 MS.gene061543:CDS 45.0%
TGGGTGTGAGGAGAAAACAA+AGG - chr3.2:43859317-43859336 MS.gene061543:CDS 45.0%
TTAACCTCGTACGCTGAAAC+AGG + chr3.2:43858474-43858493 None:intergenic 45.0%
TTCAGCGTACGAGGTTAATG+AGG - chr3.2:43858476-43858495 MS.gene061543:CDS 45.0%
! TATGTTGCAAGTTGGGTGTG+AGG - chr3.2:43859305-43859324 MS.gene061543:CDS 45.0%
!! GGGATATGTGAAAAGGCACT+AGG - chr3.2:43859069-43859088 MS.gene061543:CDS 45.0%
!! TGAAGGTGTTGTTGAGGGTT+TGG - chr3.2:43858882-43858901 MS.gene061543:intron 45.0%
ACTCACCGGACAACGGAAAT+GGG + chr3.2:43858229-43858248 None:intergenic 50.0%
AGTCTAGACTCACCGGACAA+CGG + chr3.2:43858236-43858255 None:intergenic 50.0%
TTTGGTGGAGAGAGAGTGAG+AGG - chr3.2:43858574-43858593 MS.gene061543:CDS 50.0%
!!! GCTTTTGAGGGTGTCTCCTT+TGG - chr3.2:43859176-43859195 MS.gene061543:CDS 50.0%
ACCGGACAACGGAAATGGGT+TGG + chr3.2:43858225-43858244 None:intergenic 55.0%
AGAAACATGTTCCGCCGCAC+CGG - chr3.2:43858135-43858154 MS.gene061543:CDS 55.0%
CCCAACCCATTTCCGTTGTC+CGG - chr3.2:43858221-43858240 MS.gene061543:CDS 55.0%
GACTCACCGGACAACGGAAA+TGG + chr3.2:43858230-43858249 None:intergenic 55.0%
! CCGGACAACGGAAATGGGTT+GGG + chr3.2:43858224-43858243 None:intergenic 55.0%
! GGTGTTTTGCTTTGCCGGTG+CGG + chr3.2:43858152-43858171 None:intergenic 55.0%
AAACACCAACTTCCCGGCGC+CGG - chr3.2:43858165-43858184 MS.gene061543:CDS 60.0%
CACCGTTAATTCGCCGTCGC+CGG + chr3.2:43858187-43858206 None:intergenic 60.0%
CGACGGCGAATTAACGGTGG+AGG - chr3.2:43858188-43858207 MS.gene061543:CDS 60.0%
! GTTTTGCTTTGCCGGTGCGG+CGG + chr3.2:43858149-43858168 None:intergenic 60.0%
CGCCGGCGACGGCGAATTAA+CGG - chr3.2:43858182-43858201 MS.gene061543:CDS 65.0%
CGGCGACGGCGAATTAACGG+TGG - chr3.2:43858185-43858204 MS.gene061543:CDS 65.0%
TAATTCGCCGTCGCCGGCGC+CGG + chr3.2:43858181-43858200 None:intergenic 70.0%
AATTCGCCGTCGCCGGCGCC+GGG + chr3.2:43858180-43858199 None:intergenic 75.0%
!! CAACTTCCCGGCGCCGGCGA+CGG - chr3.2:43858171-43858190 MS.gene061543:CDS 75.0%
!! CGTCGCCGGCGCCGGGAAGT+TGG + chr3.2:43858173-43858192 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 43858114 43859434 43858114 ID=MS.gene061543
chr3.2 mRNA 43858114 43859434 43858114 ID=MS.gene061543.t1;Parent=MS.gene061543
chr3.2 exon 43859051 43859434 43859051 ID=MS.gene061543.t1.exon1;Parent=MS.gene061543.t1
chr3.2 CDS 43859051 43859434 43859051 ID=cds.MS.gene061543.t1;Parent=MS.gene061543.t1
chr3.2 exon 43858114 43858728 43858114 ID=MS.gene061543.t1.exon2;Parent=MS.gene061543.t1
chr3.2 CDS 43858114 43858728 43858114 ID=cds.MS.gene061543.t1;Parent=MS.gene061543.t1
Gene Sequence

>MS.gene061543

ATGGTTTTATCATGGACAAAAAGAAACATGTTCCGCCGCACCGGCAAAGCAAAACACCAACTTCCCGGCGCCGGCGACGGCGAATTAACGGTGGAGGAGATAACAATCCCAACCCATTTCCGTTGTCCGGTGAGTCTAGACTTGATGAAAGATCCAGTAACTCTATCAACAGGAATCACCTACGATAGATTCAGCATCGAGAAATGGATCGAAGCTGGAAACAAAACTTGCCCAGTTACAAATCAGAAACTTTCAACCTTCGAAATCACACCAAATCACACCATAAGAAAAATGATTCAGAGTTGGTGTGTTGAAAATAGTTCTTATGGGATTGAGAGAATTCCAACACCTCGTATACCTGTTTCAGCGTACGAGGTTAATGAGGTTTGTACTAGGTTGTTGTCGGGTTGTAGAAATTTGGATGAGAAAAAGTGTGTTGAGTTTGTTGGAAAGATTAAGGTTTGGTGGAGAGAGAGTGAGAGGAATAAGAGGGTTATAATAGGAAATGGAGTTTGTTCTGTTTTAGCTACGGTTTTTGATTGTTTTTCATGTGTTTCTTTTGAGGAACATGTTGTTGTTTTGGAGGAGATTTTGGAGATTTTAACATGGATTGTTAAAACCTCTTTTAGCGATTCGAAAACGAAGATGTGTTTATCGTCTTCATCTTTGAATTGTTTAGTTTGGTTTTTAGATGGTAAAGATTTAGGGTGCAAGACAAAATGCTGTTTTGTTGCTTAAAGAAATGAATGTTGAAGAGTTATCAAGAATTGAAGGTGTTGTTGAGGGTTTGGTGAAGATTGTTAAGGAACCAATTGGGTCAAGTGCTACAAAAGCATGTTTGACTACAATTTTCAAGTTGGTTTCATCGGCGAAAAATAGAGATGAGATTAGTGAAAAGTTTGTTGAATTGGGTTTGATTTCATTTTTGCTTGAAACAATTGTTGATGGAGAAAAAGGGATATGTGAAAAGGCACTAGGTGTATTGGATTGTTTATGTGATTGTAAGAAAGGGAAAGAGGTTGTTCAAAGGAACGCTTTAGCTTTGCCTCTTGTGATTAAGAAGCTTTTGAGGGTGTCTCCTTTGGCTTCTAGTTTTGCTGTTGGGATTGTTAGGAAGATGCTTTGTGAGAAGAAGGAAGAAAGAGTTTTGATTGAAGCAATTCAACTTGGTGCTTTTCAGAAATTGTTGGTTATGTTGCAAGTTGGGTGTGAGGAGAAAACAAAGGAGGATACTACTGAGTTGTTGAAATTGTTGAATGGTTATAGAAGCAAAGCTGAATGTGTTGACAATTCATTAGATTTCAAGTATCTCAAGAACTAA
Protein sequence

>MS.gene061543.t1

MVLSWTKRNMFRRTGKAKHQLPGAGDGELTVEEITIPTHFRCPVSLDLMKDPVTLSTGITYDRFSIEKWIEAGNKTCPVTNQKLSTFEITPNHTIRKMIQSWCVENSSYGIERIPTPRIPVSAYEVNEGARQNAVLLLKEMNVEELSRIEGVVEGLVKIVKEPIGSSATKACLTTIFKLVSSAKNRDEISEKFVELGLISFLLETIVDGEKGICEKALGVLDCLCDCKKGKEVVQRNALALPLVIKKLLRVSPLASSFAVGIVRKMLCEKKEERVLIEAIQLGAFQKLLVMLQVGCEEKTKEDTTELLKLLNGYRSKAECVDNSLDFKYLKN