AlfalfaGEDB | MS.gene26139

M. sativa cultivar XinJiangDaYe / MS.gene26139

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26139.t1	XP_003616222.1	96.8	442	14	0	1	442	1	442	2.80E-237	830.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26139.t1	Q9C8D1	43.8	434	215	9	19	442	17	431	7.9e-95	349.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26139.t1	G7KFZ3	96.8	442	14	0	1	442	1	442	2.0e-237	830.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051837	MS.gene26139	0.820992	5.12E-53	-1.69E-46
MS.gene052091	MS.gene26139	0.813892	2.03E-51	-1.69E-46
MS.gene055549	MS.gene26139	0.829787	4.23E-55	-1.69E-46
MS.gene055550	MS.gene26139	0.844785	6.10E-59	-1.69E-46
MS.gene055551	MS.gene26139	0.832248	1.05E-55	-1.69E-46
MS.gene057093	MS.gene26139	0.82824	1.00E-54	-1.69E-46
MS.gene060964	MS.gene26139	0.836952	6.92E-57	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene034751	MS.gene26139	PPI
MS.gene061546	MS.gene26139	PPI
MS.gene064492	MS.gene26139	PPI
MS.gene017373	MS.gene26139	PPI
MS.gene042912	MS.gene26139	PPI
MS.gene26139	MS.gene066129	PPI
MS.gene26139	MS.gene41033	PPI
MS.gene048677	MS.gene26139	PPI
MS.gene034753	MS.gene26139	PPI
MS.gene008306	MS.gene26139	PPI
MS.gene41032	MS.gene26139	PPI
MS.gene04998	MS.gene26139	PPI
MS.gene025667	MS.gene26139	PPI
MS.gene26139	MS.gene72611	PPI
MS.gene007121	MS.gene26139	PPI
MS.gene061543	MS.gene26139	PPI
MS.gene45456	MS.gene26139	PPI
MS.gene26139	MS.gene066130	PPI
MS.gene26139	MS.gene45454	PPI
MS.gene45455	MS.gene26139	PPI
MS.gene26139	MS.gene26489	PPI
MS.gene26139	MS.gene022190	PPI
MS.gene93972	MS.gene26139	PPI
MS.gene022190	MS.gene26139	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26139.t1	MTR_5g077510	96.833	442	14	0	1	442	1	442	0.0	868
MS.gene26139.t1	MTR_3g065080	63.657	443	152	8	1	439	1	438	0.0	516
MS.gene26139.t1	MTR_6g071340	48.471	425	199	8	31	442	1	418	4.41e-129	379
MS.gene26139.t1	MTR_5g048050	48.357	426	200	8	30	442	20	438	1.42e-126	374
MS.gene26139.t1	MTR_4g085720	33.584	399	239	10	29	418	8	389	7.96e-59	198
MS.gene26139.t1	MTR_2g087350	35.766	411	231	10	32	428	3	394	2.56e-56	191
MS.gene26139.t1	MTR_7g106340	33.489	427	242	14	32	433	5	414	1.32e-54	187
MS.gene26139.t1	MTR_1g056910	32.057	418	251	12	32	433	3	403	3.10e-52	181
MS.gene26139.t1	MTR_1g056880	33.410	434	240	16	32	428	4	425	9.13e-51	177
MS.gene26139.t1	MTR_1g056870	33.562	438	234	17	32	428	4	425	1.74e-50	177
MS.gene26139.t1	MTR_1g056840	31.422	436	220	17	32	428	4	399	4.98e-45	162
MS.gene26139.t1	MTR_8g092870	28.671	429	269	17	33	442	8	418	9.13e-36	137
MS.gene26139.t1	MTR_4g051515	27.578	417	268	13	32	429	3	404	3.72e-35	135
MS.gene26139.t1	MTR_5g015210	29.825	399	229	12	35	417	67	430	2.36e-33	130
MS.gene26139.t1	MTR_3g095730	26.714	423	283	16	33	442	11	419	7.01e-33	129
MS.gene26139.t1	MTR_4g063800	28.186	408	237	17	34	419	254	627	3.71e-32	129
MS.gene26139.t1	MTR_1g017770	28.244	393	248	9	35	417	45	413	1.20e-29	120
MS.gene26139.t1	MTR_4g107010	28.271	428	266	15	15	425	267	670	3.73e-27	115
MS.gene26139.t1	MTR_4g485520	26.747	415	262	16	22	418	88	478	8.39e-27	112
MS.gene26139.t1	MTR_4g485520	26.065	399	259	14	33	418	271	646	1.13e-26	113
MS.gene26139.t1	MTR_5g083030	24.760	416	262	13	33	419	291	684	2.68e-25	109
MS.gene26139.t1	MTR_7g059405	26.650	409	237	18	35	419	264	633	7.70e-25	107
MS.gene26139.t1	MTR_7g078330	24.250	400	258	15	33	418	272	640	2.17e-23	103
MS.gene26139.t1	MTR_2g018010	25.949	316	194	9	33	329	290	584	9.62e-23	101
MS.gene26139.t1	MTR_4g028960	46.053	76	41	0	31	106	276	351	2.01e-21	97.4
MS.gene26139.t1	MTR_5g034440	25.647	425	279	11	15	424	268	670	2.35e-21	97.4
MS.gene26139.t1	MTR_3g078160	34.532	139	84	3	24	162	264	395	9.46e-21	95.5
MS.gene26139.t1	MTR_3g078160	34.532	139	84	3	24	162	264	395	1.14e-20	95.1
MS.gene26139.t1	MTR_3g078160	34.532	139	84	3	24	162	264	395	2.23e-20	94.0
MS.gene26139.t1	MTR_1g094215	26.582	395	256	13	35	418	64	435	2.24e-19	90.5
MS.gene26139.t1	MTR_1g094215	26.286	350	230	10	35	375	247	577	4.13e-19	90.5
MS.gene26139.t1	MTR_3g085610	41.667	84	43	1	36	119	281	358	4.28e-19	90.5
MS.gene26139.t1	MTR_4g091880	24.235	392	263	12	35	420	5	368	1.36e-18	87.4
MS.gene26139.t1	MTR_4g091880	48.529	68	34	1	35	101	5	72	1.61e-18	87.0
MS.gene26139.t1	MTR_1g069845	44.286	70	39	0	33	102	122	191	3.89e-18	87.4
MS.gene26139.t1	MTR_7g097020	37.805	82	51	0	21	102	227	308	4.78e-18	87.4
MS.gene26139.t1	MTR_1g069845	44.286	70	39	0	33	102	243	312	6.33e-18	87.0
MS.gene26139.t1	MTR_1g069845	44.286	70	39	0	33	102	239	308	6.84e-18	86.7
MS.gene26139.t1	MTR_2g011140	25.352	426	250	15	21	422	1	382	9.91e-17	82.0
MS.gene26139.t1	MTR_7g053260	43.478	69	39	0	33	101	76	144	1.35e-16	82.0
MS.gene26139.t1	MTR_7g116600	42.708	96	51	2	32	127	75	166	1.67e-16	81.6
MS.gene26139.t1	MTR_2g096850	42.857	70	40	0	32	101	586	655	2.42e-15	79.0
MS.gene26139.t1	MTR_2g096850	42.857	70	40	0	32	101	737	806	2.54e-15	79.0
MS.gene26139.t1	MTR_8g080280	38.095	84	46	1	36	119	283	360	2.81e-15	78.6
MS.gene26139.t1	MTR_1g079450	25.828	302	191	12	30	316	58	341	4.09e-15	77.4
MS.gene26139.t1	MTR_8g077205	41.667	72	42	0	31	102	159	230	7.80e-15	77.0
MS.gene26139.t1	MTR_8g077205	41.667	72	42	0	31	102	271	342	8.12e-15	77.4
MS.gene26139.t1	MTR_7g077780	40.000	70	42	0	36	105	827	896	1.73e-14	76.3
MS.gene26139.t1	MTR_7g117890	24.034	233	143	5	39	265	259	463	2.44e-14	75.9
MS.gene26139.t1	MTR_3g115670	31.333	150	80	4	39	183	34	165	4.25e-14	75.1
MS.gene26139.t1	MTR_5g032010	33.333	87	58	0	15	101	712	798	6.03e-14	74.7
MS.gene26139.t1	MTR_3g096370	64.444	45	16	0	27	71	2	46	1.66e-13	71.2
MS.gene26139.t1	MTR_1g100820	39.394	66	40	0	36	101	649	714	2.13e-13	72.8
MS.gene26139.t1	MTR_1g100820	39.394	66	40	0	36	101	647	712	2.16e-13	72.8
MS.gene26139.t1	MTR_5g020570	38.095	84	48	2	33	116	271	350	2.48e-13	72.8
MS.gene26139.t1	MTR_5g020570	38.095	84	48	2	33	116	286	365	2.50e-13	72.8
MS.gene26139.t1	MTR_1g090320	41.860	86	45	3	36	117	514	598	2.55e-13	72.8
MS.gene26139.t1	MTR_1g090320	41.860	86	45	3	36	117	516	600	2.65e-13	72.8
MS.gene26139.t1	MTR_3g466220	37.143	70	44	0	33	102	231	300	6.75e-13	71.2
MS.gene26139.t1	MTR_3g466220	37.143	70	44	0	33	102	210	279	7.68e-13	71.2
MS.gene26139.t1	MTR_3g008270	43.939	66	36	1	36	101	731	795	2.47e-12	69.3
MS.gene26139.t1	MTR_8g103227	43.284	67	37	1	36	101	396	462	3.68e-12	69.3
MS.gene26139.t1	MTR_7g005940	42.857	70	37	2	34	101	594	662	4.25e-12	68.9
MS.gene26139.t1	MTR_7g005940	42.857	70	37	2	34	101	594	662	4.32e-12	68.9
MS.gene26139.t1	MTR_1g093965	45.312	64	32	1	48	111	1	61	5.63e-12	65.1
MS.gene26139.t1	MTR_1g093995	45.312	64	32	1	48	111	1	61	7.35e-12	64.7
MS.gene26139.t1	MTR_8g027140	23.707	232	145	6	39	265	263	467	1.11e-11	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26139.t1	AT1G66160	44.045	445	218	11	10	442	6	431	3.49e-122	362
MS.gene26139.t1	AT5G37490	44.737	418	214	8	32	442	28	435	1.24e-111	335
MS.gene26139.t1	AT1G66160	41.798	445	212	12	10	442	6	415	2.17e-109	329
MS.gene26139.t1	AT3G02840	41.504	359	180	11	103	442	32	379	3.13e-71	230
MS.gene26139.t1	AT2G35930	35.075	402	225	12	32	417	9	390	1.60e-59	200
MS.gene26139.t1	AT3G19380	32.481	391	235	10	29	405	8	383	2.62e-58	197
MS.gene26139.t1	AT1G49780	32.587	402	248	9	29	418	8	398	1.40e-54	187
MS.gene26139.t1	AT3G52450	32.438	447	239	13	32	434	4	431	5.11e-54	186
MS.gene26139.t1	AT3G11840	30.159	441	257	14	32	429	7	439	1.21e-42	156
MS.gene26139.t1	AT5G64660	27.506	429	276	16	33	442	8	420	1.26e-35	136
MS.gene26139.t1	AT3G49810	28.283	396	262	6	28	423	60	433	2.10e-33	130
MS.gene26139.t1	AT5G65920	27.110	391	266	5	35	425	60	431	1.31e-32	129
MS.gene26139.t1	AT5G65920	27.110	391	266	5	35	425	60	431	1.31e-32	129
MS.gene26139.t1	AT5G09800	27.027	407	260	13	27	418	4	388	3.10e-32	127
MS.gene26139.t1	AT2G28830	25.735	408	258	16	36	424	185	566	1.71e-27	115
MS.gene26139.t1	AT3G18710	26.087	437	277	17	27	442	4	415	7.20e-27	112
MS.gene26139.t1	AT1G23030	26.633	398	253	14	32	418	238	607	4.92e-26	111
MS.gene26139.t1	AT3G46510	26.000	400	248	15	35	416	256	625	2.87e-25	109
MS.gene26139.t1	AT1G71020	27.494	411	240	17	33	418	93	470	1.26e-24	106
MS.gene26139.t1	AT1G71020	27.494	411	240	17	33	418	241	618	1.82e-24	106
MS.gene26139.t1	AT1G10560	26.044	407	257	10	41	424	294	679	4.13e-23	102
MS.gene26139.t1	AT3G54850	25.061	407	248	13	35	418	248	620	1.58e-22	100
MS.gene26139.t1	AT5G42340	30.625	160	106	2	35	193	290	445	5.49e-22	99.4
MS.gene26139.t1	AT5G01830	52.941	68	32	0	35	102	274	341	5.34e-19	90.1
MS.gene26139.t1	AT1G29340	35.811	148	84	4	33	170	303	449	7.49e-19	89.7
MS.gene26139.t1	AT1G60190	22.807	399	283	9	39	424	282	668	1.07e-18	89.4
MS.gene26139.t1	AT4G21350	48.529	68	34	1	35	101	5	72	2.05e-17	84.0
MS.gene26139.t1	AT1G27910	38.235	102	51	2	36	128	280	378	1.13e-16	83.2
MS.gene26139.t1	AT3G54790	43.243	74	42	0	33	106	200	273	1.34e-16	82.8
MS.gene26139.t1	AT3G54790	43.243	74	42	0	33	106	236	309	1.58e-16	82.8
MS.gene26139.t1	AT3G54790	43.243	74	42	0	33	106	236	309	1.58e-16	82.8
MS.gene26139.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.62e-16	82.8
MS.gene26139.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.62e-16	82.8
MS.gene26139.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.62e-16	82.8
MS.gene26139.t1	AT3G07360	43.478	92	51	1	11	102	51	141	1.85e-16	81.6
MS.gene26139.t1	AT1G67530	41.463	82	47	1	24	105	262	342	2.59e-16	81.6
MS.gene26139.t1	AT1G24330	42.857	70	40	0	36	105	276	345	3.03e-16	81.6
MS.gene26139.t1	AT1G24330	42.857	70	40	0	36	105	276	345	3.03e-16	81.6
MS.gene26139.t1	AT2G45920	32.407	108	59	2	8	101	284	391	2.41e-15	77.8
MS.gene26139.t1	AT5G57035	37.363	91	54	1	19	106	689	779	2.42e-15	79.0
MS.gene26139.t1	AT4G36550	40.845	71	42	0	35	105	219	289	2.50e-15	79.0
MS.gene26139.t1	AT3G49060	36.585	82	52	0	23	104	690	771	1.08e-14	77.0
MS.gene26139.t1	AT3G49060	36.585	82	52	0	23	104	723	804	1.11e-14	77.0
MS.gene26139.t1	AT3G49060	36.585	82	52	0	23	104	713	794	1.20e-14	76.6
MS.gene26139.t1	AT1G01680	35.789	95	60	1	15	108	212	306	1.35e-14	74.7
MS.gene26139.t1	AT1G01660	37.879	66	41	0	36	101	499	564	1.80e-14	75.9
MS.gene26139.t1	AT1G01660	37.879	66	41	0	36	101	498	563	1.90e-14	75.9
MS.gene26139.t1	AT3G61390	42.424	66	38	0	36	101	279	344	2.41e-14	74.7
MS.gene26139.t1	AT3G61390	42.424	66	38	0	36	101	354	419	3.31e-14	74.7
MS.gene26139.t1	AT5G65500	35.227	88	57	0	14	101	694	781	2.68e-13	72.4
MS.gene26139.t1	AT5G65500	35.227	88	57	0	14	101	702	789	2.87e-13	72.4
MS.gene26139.t1	AT2G45910	37.143	70	44	0	36	105	764	833	5.42e-13	71.6
MS.gene26139.t1	AT2G45910	37.143	70	44	0	36	105	764	833	5.42e-13	71.6
MS.gene26139.t1	AT1G20780	45.588	68	32	1	39	101	27	94	1.09e-12	70.9
MS.gene26139.t1	AT1G20780	45.588	68	32	1	39	101	27	94	1.09e-12	70.9
MS.gene26139.t1	AT1G20780	45.588	68	32	1	39	101	27	94	1.09e-12	70.9
MS.gene26139.t1	AT5G51270	43.939	66	36	1	36	101	736	800	1.28e-12	70.5
MS.gene26139.t1	AT5G51270	43.939	66	36	1	36	101	736	800	1.28e-12	70.5
MS.gene26139.t1	AT2G23140	38.571	70	43	0	33	102	231	300	1.29e-12	70.5
MS.gene26139.t1	AT2G23140	38.571	70	43	0	33	102	228	297	1.34e-12	70.5
MS.gene26139.t1	AT2G23140	38.571	70	43	0	33	102	228	297	1.34e-12	70.5
MS.gene26139.t1	AT2G23140	38.571	70	43	0	33	102	39	108	1.76e-12	69.7
MS.gene26139.t1	AT2G23140	38.571	70	43	0	33	102	39	108	1.76e-12	69.7
MS.gene26139.t1	AT1G56040	41.538	65	38	0	36	100	354	418	1.94e-12	69.3
MS.gene26139.t1	AT1G01670	36.000	75	48	0	31	105	206	280	2.50e-12	67.8
MS.gene26139.t1	AT5G67340	35.000	80	52	0	29	108	234	313	3.04e-12	69.3
MS.gene26139.t1	AT5G67340	35.000	80	52	0	29	108	234	313	3.04e-12	69.3
MS.gene26139.t1	AT2G19410	34.884	86	52	2	19	101	692	776	3.69e-12	68.9
MS.gene26139.t1	AT2G19410	34.884	86	52	2	19	101	706	790	3.76e-12	68.9
MS.gene26139.t1	AT2G19410	34.884	86	52	2	19	101	727	811	4.11e-12	68.9
MS.gene26139.t1	AT1G01670	36.000	75	48	0	31	105	290	364	5.14e-12	67.4
MS.gene26139.t1	AT1G01670	36.000	75	48	0	31	105	290	364	5.14e-12	67.4
MS.gene26139.t1	AT1G56030	40.000	65	39	0	36	100	324	388	5.37e-12	67.8
MS.gene26139.t1	AT1G76390	41.379	87	43	3	23	101	10	96	8.04e-12	67.8
MS.gene26139.t1	AT1G76390	41.379	87	43	3	23	101	10	96	8.04e-12	67.8
MS.gene26139.t1	AT4G25160	40.845	71	41	1	31	101	762	831	1.10e-11	67.4
MS.gene26139.t1	AT5G61560	40.580	69	40	1	36	104	724	791	1.61e-11	67.0
MS.gene26139.t1	AT5G61560	40.580	69	40	1	36	104	726	793	1.62e-11	67.0
MS.gene26139.t1	AT5G61560	40.580	69	40	1	36	104	726	793	1.62e-11	67.0
MS.gene26139.t1	AT5G61560	40.580	69	40	1	36	104	745	812	1.64e-11	67.0
MS.gene26139.t1	AT5G61560	40.580	69	40	1	36	104	745	812	1.64e-11	67.0

Find 71 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGTATAAAAGTGATTT+TGG	0.163488	5.4:-57136699	MS.gene26139:CDS
TTCAAATTCTTTGTCTTGTT+TGG	0.197820	5.4:-57136921	MS.gene26139:CDS
TGAGAAGAACGTTGTTCTTT+TGG	0.257010	5.4:-57137014	MS.gene26139:CDS
ATTCTTGTTGATGCTGAAAA+AGG	0.259858	5.4:-57136635	MS.gene26139:CDS
TTCATCAAGTCTAGAGTTAC+TGG	0.261782	5.4:+57137448	None:intergenic
TGAATTGGTGAGAAAGATAA+AGG	0.287993	5.4:-57137137	MS.gene26139:CDS
AAATTCTTCTTTGTTGCTTA+AGG	0.289781	5.4:-57136858	MS.gene26139:CDS
TTTGGAGAAGTTTGTTGAAT+TGG	0.298309	5.4:-57136681	MS.gene26139:CDS
TGAAGGGATTGTTGAATCTT+TGG	0.314720	5.4:-57136807	MS.gene26139:CDS
ATTCTTTGTCTTGTTTGGTT+TGG	0.328507	5.4:-57136916	MS.gene26139:CDS
TTGGAGAAGTTTGTTGAATT+GGG	0.341918	5.4:-57136680	MS.gene26139:CDS
GTAACTTCATAACGAGTTAC+TGG	0.352002	5.4:+57137217	None:intergenic
GGGTTTGGTTTCAATTCTAT+TGG	0.353199	5.4:-57136660	MS.gene26139:CDS
GTTAAGGTTAATGTTGAGAT+TGG	0.360051	5.4:-57136776	MS.gene26139:CDS
GGAGTTTGTGAGAAAGCTTT+AGG	0.360911	5.4:-57136614	MS.gene26139:CDS
AAGTTACTGATACGTGTAAT+AGG	0.361648	5.4:-57137201	MS.gene26139:CDS
GTGTGTTGATTCATCATCAT+TGG	0.376962	5.4:-57136294	MS.gene26139:CDS
TATCATGGACAAAAGGTAGT+AGG	0.385260	5.4:-57137564	MS.gene26139:CDS
TAAATTAGAATGGCTTCTTA+AGG	0.391586	5.4:+57136261	None:intergenic
GAAGTTTGTTGAATTGGGTT+TGG	0.405985	5.4:-57136675	MS.gene26139:CDS
ATCTTTGGTGAACATGGTTA+AGG	0.411892	5.4:-57136792	MS.gene26139:CDS
GTCTCTATCGTAGGTTATGC+CGG	0.413834	5.4:+57137405	None:intergenic
TGCTCCTCTTGTTCTTGCTA+AGG	0.421484	5.4:-57137065	MS.gene26139:CDS
GGTGAGAAAGATAAAGGGTT+GGG	0.424541	5.4:-57137131	MS.gene26139:CDS
ATGCCGGTTGAAAGTGTAAC+GGG	0.424846	5.4:+57137421	None:intergenic
GGAAATGAATGTTGAGTCAT+TGG	0.428757	5.4:-57136837	MS.gene26139:CDS
ATTCTACGAAGGGAGTGATT+TGG	0.431522	5.4:+57137304	None:intergenic
TTCGTAGAATGATTCAAGAT+TGG	0.433178	5.4:-57137291	MS.gene26139:CDS
TTGATTGAGGCAATTCAACT+TGG	0.459886	5.4:-57136425	MS.gene26139:CDS
TTAAGGTTAATGTTGAGATT+GGG	0.460559	5.4:-57136775	MS.gene26139:CDS
AAGAAGGTTATAGTTTCAAA+TGG	0.465955	5.4:-57137088	MS.gene26139:CDS
GACCCCGTTACACTTTCAAC+CGG	0.468981	5.4:-57137424	MS.gene26139:CDS
GAATTGGTGAGAAAGATAAA+GGG	0.484864	5.4:-57137136	MS.gene26139:CDS
TTACTGGAATACGTGGAGTT+GGG	0.486770	5.4:+57137233	None:intergenic
CAATTCAGTAGCTTTCTCCT+TGG	0.500670	5.4:+57136352	None:intergenic
ATCATGGACAAAAGGTAGTA+GGG	0.502085	5.4:-57137563	MS.gene26139:CDS
TCCCCATAGATAAATTAGAA+TGG	0.503592	5.4:+57136251	None:intergenic
TGTGTTCAACACCGTTCCTA+TGG	0.506903	5.4:-57137268	MS.gene26139:CDS
TTCTTTCGACGCCATAGGAA+CGG	0.509833	5.4:+57137257	None:intergenic
GAGTCATTGGTGAAAATTGA+AGG	0.521116	5.4:-57136824	MS.gene26139:CDS
TTCGATGCTGTCTCTATCGT+AGG	0.522127	5.4:+57137396	None:intergenic
GTTACTGGAATACGTGGAGT+TGG	0.523278	5.4:+57137232	None:intergenic
GAATTGTTGAAATTGTTGAA+TGG	0.533871	5.4:-57136335	MS.gene26139:CDS
TACGTGTAATAGGATTCTTT+CGG	0.539106	5.4:-57137191	MS.gene26139:CDS
GTTGGGGAAAAGAGAGTGAA+AGG	0.542116	5.4:-57137114	MS.gene26139:CDS
AAAAGCTTCTTAATAACAAG+AGG	0.544112	5.4:+57136524	None:intergenic
TGTTGAATCTTTGGTGAACA+TGG	0.547292	5.4:-57136798	MS.gene26139:CDS
AGAGAGTGAAAGGAACAAGA+AGG	0.547893	5.4:-57137104	MS.gene26139:CDS
AGTCATTGGTGAAAATTGAA+GGG	0.551451	5.4:-57136823	MS.gene26139:CDS
CAAGATTTGTGACAAGGCTG+AGG	0.555526	5.4:-57136456	MS.gene26139:CDS
ATCTTGAATCATTCTACGAA+GGG	0.557256	5.4:+57137294	None:intergenic
TGATTGAGGCAATTCAACTT+GGG	0.559461	5.4:-57136424	MS.gene26139:CDS
TGGGATTCTTTCGACGCCAT+AGG	0.559846	5.4:+57137252	None:intergenic
TTCATGCAATGATTTACAAG+TGG	0.567554	5.4:-57137500	MS.gene26139:CDS
GTGAGAAAGATAAAGGGTTG+GGG	0.581859	5.4:-57137130	MS.gene26139:CDS
ATTCTTTCGGCGGCGCAACA+AGG	0.583900	5.4:-57137178	MS.gene26139:CDS
ACTGGGCAACGAAAATGAGT+TGG	0.587838	5.4:+57137466	None:intergenic
AAAACCTTAGCAAGAACAAG+AGG	0.596665	5.4:+57137061	None:intergenic
GTGATTTGGTACGATGTCAA+AGG	0.600729	5.4:+57137318	None:intergenic
TCATCAAGTCTAGAGTTACT+GGG	0.601581	5.4:+57137449	None:intergenic
TGTTTACAAGATTTGTGACA+AGG	0.613076	5.4:-57136462	MS.gene26139:CDS
GTGTAATAGGATTCTTTCGG+CGG	0.616603	5.4:-57137188	MS.gene26139:CDS
AATCTTGAATCATTCTACGA+AGG	0.617281	5.4:+57137293	None:intergenic
TGGTGAGAAAGATAAAGGGT+TGG	0.619284	5.4:-57137132	MS.gene26139:CDS
GATGCGTCCTTTGTCTGAAG+AGG	0.622117	5.4:-57136966	MS.gene26139:CDS
ATGCGTCCTTTGTCTGAAGA+GGG	0.633362	5.4:-57136965	MS.gene26139:CDS
AATCTACCCTCTTCAGACAA+AGG	0.640481	5.4:+57136959	None:intergenic
TATGCCGGTTGAAAGTGTAA+CGG	0.642906	5.4:+57137420	None:intergenic
TAACGAGTTACTGGAATACG+TGG	0.686345	5.4:+57137226	None:intergenic
AGGTTGTGCTGAGAGTACCA+AGG	0.750134	5.4:-57136369	MS.gene26139:CDS
TGCCGGTTGAAAGTGTAACG+GGG	0.753225	5.4:+57137422	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCAACAAAAAATTTCAACT+AGG	-	chr5.4:57136953-57136972	MS.gene26139:CDS	20.0%
!!!	AAAGAGTATAAAAGTGATTT+TGG	-	chr5.4:57137137-57137156	MS.gene26139:CDS	20.0%
!	AAAAGCTTCTTAATAACAAG+AGG	+	chr5.4:57137315-57137334	None:intergenic	25.0%
!	AAGAAGGTTATAGTTTCAAA+TGG	-	chr5.4:57136748-57136767	MS.gene26139:CDS	25.0%
!	GAATTGTTGAAATTGTTGAA+TGG	-	chr5.4:57137501-57137520	MS.gene26139:CDS	25.0%
!	GTCTTTGTGATAGTAAAAAT+GGG	-	chr5.4:57137256-57137275	MS.gene26139:CDS	25.0%
!	TCTTTGTGATAGTAAAAATG+GGG	-	chr5.4:57137257-57137276	MS.gene26139:CDS	25.0%
!	TGTCTTTGTGATAGTAAAAA+TGG	-	chr5.4:57137255-57137274	MS.gene26139:CDS	25.0%
!	TTAAGGTTAATGTTGAGATT+GGG	-	chr5.4:57137061-57137080	MS.gene26139:CDS	25.0%
!!	AAATTCTTCTTTGTTGCTTA+AGG	-	chr5.4:57136978-57136997	MS.gene26139:CDS	25.0%
!!	TGTTATTAAGAAGCTTTTGA+GGG	-	chr5.4:57137317-57137336	MS.gene26139:CDS	25.0%
!!	TTCAAATTCTTTGTCTTGTT+TGG	-	chr5.4:57136915-57136934	MS.gene26139:CDS	25.0%
!!	TTTGTCAACAATTTTTCACT+TGG	-	chr5.4:57137104-57137123	MS.gene26139:CDS	25.0%
!!!	TGTTAACTCTGTTTTTGTAA+CGG	+	chr5.4:57136497-57136516	None:intergenic	25.0%
!!!	TTGTTATTAAGAAGCTTTTG+AGG	-	chr5.4:57137316-57137335	MS.gene26139:CDS	25.0%
	AAGTTACTGATACGTGTAAT+AGG	-	chr5.4:57136635-57136654	MS.gene26139:CDS	30.0%
	AATCTTGAATCATTCTACGA+AGG	+	chr5.4:57136546-57136565	None:intergenic	30.0%
	AGTCATTGGTGAAAATTGAA+GGG	-	chr5.4:57137013-57137032	MS.gene26139:CDS	30.0%
	ATCGAAAAATGGTTTGAATC+AGG	-	chr5.4:57136457-57136476	MS.gene26139:CDS	30.0%
	ATCTTGAATCATTCTACGAA+GGG	+	chr5.4:57136545-57136564	None:intergenic	30.0%
	ATTCTTGTTGATGCTGAAAA+AGG	-	chr5.4:57137201-57137220	MS.gene26139:CDS	30.0%
	GAATTGGTGAGAAAGATAAA+GGG	-	chr5.4:57136700-57136719	MS.gene26139:CDS	30.0%
	GTTAAGGTTAATGTTGAGAT+TGG	-	chr5.4:57137060-57137079	MS.gene26139:CDS	30.0%
	TACGTGTAATAGGATTCTTT+CGG	-	chr5.4:57136645-57136664	MS.gene26139:CDS	30.0%
	TGAATTGGTGAGAAAGATAA+AGG	-	chr5.4:57136699-57136718	MS.gene26139:CDS	30.0%
	TGTTTACAAGATTTGTGACA+AGG	-	chr5.4:57137374-57137393	MS.gene26139:CDS	30.0%
	TTCATGCAATGATTTACAAG+TGG	-	chr5.4:57136336-57136355	MS.gene26139:CDS	30.0%
	TTCGTAGAATGATTCAAGAT+TGG	-	chr5.4:57136545-57136564	MS.gene26139:CDS	30.0%
!!	ATTCTTTGTCTTGTTTGGTT+TGG	-	chr5.4:57136920-57136939	MS.gene26139:CDS	30.0%
!!	TGTTCTTTTGGAAGAGATTT+TGG	-	chr5.4:57136834-57136853	MS.gene26139:CDS	30.0%
!!	TTGGAGAAGTTTGTTGAATT+GGG	-	chr5.4:57137156-57137175	MS.gene26139:CDS	30.0%
!!	TTTGGAGAAGTTTGTTGAAT+TGG	-	chr5.4:57137155-57137174	MS.gene26139:CDS	30.0%
!!!	ATGGTTTTATCATGGACAAA+AGG	-	chr5.4:57136265-57136284	MS.gene26139:CDS	30.0%
!!!	GTTAACTCTGTTTTTGTAAC+GGG	+	chr5.4:57136496-57136515	None:intergenic	30.0%
!!!	TGAGGAAGATATTTTGATTG+AGG	-	chr5.4:57137398-57137417	MS.gene26139:CDS	30.0%
	AAAACCTTAGCAAGAACAAG+AGG	+	chr5.4:57136778-57136797	None:intergenic	35.0%
	ATCATGGACAAAAGGTAGTA+GGG	-	chr5.4:57136273-57136292	MS.gene26139:CDS	35.0%
	ATCTTTGGTGAACATGGTTA+AGG	-	chr5.4:57137044-57137063	MS.gene26139:CDS	35.0%
	GAGTCATTGGTGAAAATTGA+AGG	-	chr5.4:57137012-57137031	MS.gene26139:CDS	35.0%
	GGAAATGAATGTTGAGTCAT+TGG	-	chr5.4:57136999-57137018	MS.gene26139:CDS	35.0%
	GTAACTTCATAACGAGTTAC+TGG	+	chr5.4:57136622-57136641	None:intergenic	35.0%
	TATCATGGACAAAAGGTAGT+AGG	-	chr5.4:57136272-57136291	MS.gene26139:CDS	35.0%
	TGAAGGGATTGTTGAATCTT+TGG	-	chr5.4:57137029-57137048	MS.gene26139:CDS	35.0%
	TGATTGAGGCAATTCAACTT+GGG	-	chr5.4:57137412-57137431	MS.gene26139:CDS	35.0%
	TGTTGAATCTTTGGTGAACA+TGG	-	chr5.4:57137038-57137057	MS.gene26139:CDS	35.0%
	TTGATTGAGGCAATTCAACT+TGG	-	chr5.4:57137411-57137430	MS.gene26139:CDS	35.0%
!	AGAGGGTAGATTTGTCTTTT+TGG	-	chr5.4:57136888-57136907	MS.gene26139:CDS	35.0%
!	ATAGAGACAGCATCGAAAAA+TGG	-	chr5.4:57136446-57136465	MS.gene26139:CDS	35.0%
!	GAGGGTAGATTTGTCTTTTT+GGG	-	chr5.4:57136889-57136908	MS.gene26139:CDS	35.0%
!	GGGTTTGGTTTCAATTCTAT+TGG	-	chr5.4:57137176-57137195	MS.gene26139:CDS	35.0%
!	TCATCAAGTCTAGAGTTACT+GGG	+	chr5.4:57136390-57136409	None:intergenic	35.0%
!	TGAGAAGAACGTTGTTCTTT+TGG	-	chr5.4:57136822-57136841	MS.gene26139:CDS	35.0%
!	TTCATCAAGTCTAGAGTTAC+TGG	+	chr5.4:57136391-57136410	None:intergenic	35.0%
!!	GAAGTTTGTTGAATTGGGTT+TGG	-	chr5.4:57137161-57137180	MS.gene26139:CDS	35.0%
!!	GTGTGTTGATTCATCATCAT+TGG	-	chr5.4:57137542-57137561	MS.gene26139:CDS	35.0%
!!	TGAGAGCAAGTGTTTTGAAT+TGG	-	chr5.4:57136684-57136703	MS.gene26139:CDS	35.0%
!!!	CTACTTGTTTTGTTGCAAGT+AGG	-	chr5.4:57137447-57137466	MS.gene26139:CDS	35.0%
	AATCTACCCTCTTCAGACAA+AGG	+	chr5.4:57136880-57136899	None:intergenic	40.0%
	AGAGAGTGAAAGGAACAAGA+AGG	-	chr5.4:57136732-57136751	MS.gene26139:CDS	40.0%
	ATTCTACGAAGGGAGTGATT+TGG	+	chr5.4:57136535-57136554	None:intergenic	40.0%
	CAATTCAGTAGCTTTCTCCT+TGG	+	chr5.4:57137487-57137506	None:intergenic	40.0%
	GGAGTTTGTGAGAAAGCTTT+AGG	-	chr5.4:57137222-57137241	MS.gene26139:CDS	40.0%
	GGTGAGAAAGATAAAGGGTT+GGG	-	chr5.4:57136705-57136724	MS.gene26139:CDS	40.0%
	GTGAGAAAGATAAAGGGTTG+GGG	-	chr5.4:57136706-57136725	MS.gene26139:CDS	40.0%
	GTGTAATAGGATTCTTTCGG+CGG	-	chr5.4:57136648-57136667	MS.gene26139:CDS	40.0%
	TAACGAGTTACTGGAATACG+TGG	+	chr5.4:57136613-57136632	None:intergenic	40.0%
	TGGTGAGAAAGATAAAGGGT+TGG	-	chr5.4:57136704-57136723	MS.gene26139:CDS	40.0%
	TTACTGGAATACGTGGAGTT+GGG	+	chr5.4:57136606-57136625	None:intergenic	40.0%
!	CTTTTCCTTTTCTTGCACGA+CGG	+	chr5.4:57136303-57136322	None:intergenic	40.0%
!	TATGCCGGTTGAAAGTGTAA+CGG	+	chr5.4:57136419-57136438	None:intergenic	40.0%
!!	GTGATTTGGTACGATGTCAA+AGG	+	chr5.4:57136521-57136540	None:intergenic	40.0%
!!	GTTTTTGATTCTTTCTCGCG+TGG	-	chr5.4:57136793-57136812	MS.gene26139:CDS	40.0%
!!!	AGATTTTGGAAGTGCTGACA+TGG	-	chr5.4:57136848-57136867	MS.gene26139:CDS	40.0%
	ACTGGGCAACGAAAATGAGT+TGG	+	chr5.4:57136373-57136392	None:intergenic	45.0%
	ATGCGTCCTTTGTCTGAAGA+GGG	-	chr5.4:57136871-57136890	MS.gene26139:CDS	45.0%
	CAAGATTTGTGACAAGGCTG+AGG	-	chr5.4:57137380-57137399	MS.gene26139:CDS	45.0%
	GTCTCTATCGTAGGTTATGC+CGG	+	chr5.4:57136434-57136453	None:intergenic	45.0%
	GTTACTGGAATACGTGGAGT+TGG	+	chr5.4:57136607-57136626	None:intergenic	45.0%
	GTTGGGGAAAAGAGAGTGAA+AGG	-	chr5.4:57136722-57136741	MS.gene26139:CDS	45.0%
	TGCTCCTCTTGTTCTTGCTA+AGG	-	chr5.4:57136771-57136790	MS.gene26139:CDS	45.0%
	TGTGTTCAACACCGTTCCTA+TGG	-	chr5.4:57136568-57136587	MS.gene26139:CDS	45.0%
	TTCGATGCTGTCTCTATCGT+AGG	+	chr5.4:57136443-57136462	None:intergenic	45.0%
	TTCTTTCGACGCCATAGGAA+CGG	+	chr5.4:57136582-57136601	None:intergenic	45.0%
!	ATGCCGGTTGAAAGTGTAAC+GGG	+	chr5.4:57136418-57136437	None:intergenic	45.0%
!	GTTTTCCGTCGTGCAAGAAA+AGG	-	chr5.4:57136295-57136314	MS.gene26139:CDS	45.0%
	GACCCCGTTACACTTTCAAC+CGG	-	chr5.4:57136412-57136431	MS.gene26139:CDS	50.0%
	GATGCGTCCTTTGTCTGAAG+AGG	-	chr5.4:57136870-57136889	MS.gene26139:CDS	50.0%
	TGGGATTCTTTCGACGCCAT+AGG	+	chr5.4:57136587-57136606	None:intergenic	50.0%
!	TGCCGGTTGAAAGTGTAACG+GGG	+	chr5.4:57136417-57136436	None:intergenic	50.0%
!!	AGGTTGTGCTGAGAGTACCA+AGG	-	chr5.4:57137467-57137486	MS.gene26139:CDS	50.0%
	ATTCTTTCGGCGGCGCAACA+AGG	-	chr5.4:57136658-57136677	MS.gene26139:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	57136265	57137593	57136265	ID=MS.gene26139
chr5.4	mRNA	57136265	57137593	57136265	ID=MS.gene26139.t1;Parent=MS.gene26139
chr5.4	exon	57136265	57137593	57136265	ID=MS.gene26139.t1.exon1;Parent=MS.gene26139.t1
chr5.4	CDS	57136265	57137593	57136265	ID=cds.MS.gene26139.t1;Parent=MS.gene26139.t1

Gene Sequence

>MS.gene26139

ATGGTTTTATCATGGACAAAAGGTAGTAGGGTTTTCCGTCGTGCAAGAAAAGGAAAAGAGTTAATATCAAGTTCATGCAATGATTTACAAGTGGAGATAGCAATTCCAACTCATTTTCGTTGCCCAGTAACTCTAGACTTGATGAAAGACCCCGTTACACTTTCAACCGGCATAACCTACGATAGAGACAGCATCGAAAAATGGTTTGAATCAGGTAACGACACATGTCCCGTTACAAAAACAGAGTTAACATCCTTTGACATCGTACCAAATCACTCCCTTCGTAGAATGATTCAAGATTGGTGTGTTCAACACCGTTCCTATGGCGTCGAAAGAATCCCAACTCCACGTATTCCAGTAACTCGTTATGAAGTTACTGATACGTGTAATAGGATTCTTTCGGCGGCGCAACAAGGAGATGAGAGCAAGTGTTTTGAATTGGTGAGAAAGATAAAGGGTTGGGGAAAAGAGAGTGAAAGGAACAAGAAGGTTATAGTTTCAAATGGTGCTCCTCTTGTTCTTGCTAAGGTTTTTGATTCTTTCTCGCGTGGTTTGATTGAGAAGAACGTTGTTCTTTTGGAAGAGATTTTGGAAGTGCTGACATGGATGCGTCCTTTGTCTGAAGAGGGTAGATTTGTCTTTTTGGGATCTTCAAATTCTTTGTCTTGTTTGGTTTGGTTCTTAAATGATCAACAAAAAATTTCAACTAGGCAAAATTCTTCTTTGTTGCTTAAGGAAATGAATGTTGAGTCATTGGTGAAAATTGAAGGGATTGTTGAATCTTTGGTGAACATGGTTAAGGTTAATGTTGAGATTGGGACTGCTTCTACAAAAGCATGTTTGTCAACAATTTTTCACTTGGTTTACTCATCAAAGAGTATAAAAGTGATTTTGGAGAAGTTTGTTGAATTGGGTTTGGTTTCAATTCTATTGGAGATTCTTGTTGATGCTGAAAAAGGAGTTTGTGAGAAAGCTTTAGGTGTGTTGAATTGTCTTTGTGATAGTAAAAATGGGGTGCAAATAGCAAAATCAAATGCTTTGACTTTGCCTCTTGTTATTAAGAAGCTTTTGAGGGTTTCTGAATTGAGTTCTAGTTTTGTTGTTTCTATTGTTTACAAGATTTGTGACAAGGCTGAGGAAGATATTTTGATTGAGGCAATTCAACTTGGGATGTTTCAAAAACTACTTGTTTTGTTGCAAGTAGGTTGTGCTGAGAGTACCAAGGAGAAAGCTACTGAATTGTTGAAATTGTTGAATGGTTATAAAAGCAAAGCAGAGTGTGTTGATTCATCATCATTGGATTTCACACACCTTAAGAAGCCATTCTAA

Protein sequence

>MS.gene26139.t1

MVLSWTKGSRVFRRARKGKELISSSCNDLQVEIAIPTHFRCPVTLDLMKDPVTLSTGITYDRDSIEKWFESGNDTCPVTKTELTSFDIVPNHSLRRMIQDWCVQHRSYGVERIPTPRIPVTRYEVTDTCNRILSAAQQGDESKCFELVRKIKGWGKESERNKKVIVSNGAPLVLAKVFDSFSRGLIEKNVVLLEEILEVLTWMRPLSEEGRFVFLGSSNSLSCLVWFLNDQQKISTRQNSSLLLKEMNVESLVKIEGIVESLVNMVKVNVEIGTASTKACLSTIFHLVYSSKSIKVILEKFVELGLVSILLEILVDAEKGVCEKALGVLNCLCDSKNGVQIAKSNALTLPLVIKKLLRVSELSSSFVVSIVYKICDKAEEDILIEAIQLGMFQKLLVLLQVGCAESTKEKATELLKLLNGYKSKAECVDSSSLDFTHLKKPF