AlfalfaGEDB | MS.gene93972

M. sativa cultivar XinJiangDaYe / MS.gene93972

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93972.t1	RHN52322.1	97.5	438	11	0	1	438	1	438	1.60E-245	858.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93972.t1	Q5PNY6	43.7	442	230	6	5	438	5	435	3.9e-94	346.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93972.t1	A0A396HG48	97.5	438	11	0	1	438	1	438	1.2e-245	858.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene93972	0.807826	4.18E-50	-1.69E-46
MS.gene04998	MS.gene93972	0.863387	2.58E-64	-1.69E-46
MS.gene050359	MS.gene93972	0.803077	4.14E-49	-1.69E-46
MS.gene050414	MS.gene93972	0.825008	5.92E-54	-1.69E-46
MS.gene051226	MS.gene93972	0.845162	4.82E-59	-1.69E-46
MS.gene051699	MS.gene93972	0.812055	5.14E-51	-1.69E-46
MS.gene052016	MS.gene93972	0.830688	2.55E-55	-1.69E-46
MS.gene052296	MS.gene93972	0.814681	1.36E-51	-1.69E-46
MS.gene052493	MS.gene93972	0.801849	7.41E-49	-1.69E-46
MS.gene052533	MS.gene93972	0.825547	4.42E-54	-1.69E-46
MS.gene053298	MS.gene93972	0.828721	7.68E-55	-1.69E-46
MS.gene053879	MS.gene93972	0.806941	6.44E-50	-1.69E-46
MS.gene053963	MS.gene93972	0.804594	2.00E-49	-1.69E-46
MS.gene054222	MS.gene93972	0.804959	1.68E-49	-1.69E-46
MS.gene054892	MS.gene93972	0.872583	2.84E-67	-1.69E-46
MS.gene054893	MS.gene93972	0.874636	5.79E-68	-1.69E-46
MS.gene056677	MS.gene93972	0.806009	1.01E-49	-1.69E-46
MS.gene057093	MS.gene93972	0.810749	9.87E-51	-1.69E-46
MS.gene057102	MS.gene93972	0.80351	3.37E-49	-1.69E-46
MS.gene057622	MS.gene93972	0.850873	1.28E-60	-1.69E-46
MS.gene058159	MS.gene93972	0.82435	8.47E-54	-1.69E-46
MS.gene058592	MS.gene93972	0.813834	2.09E-51	-1.69E-46
MS.gene05878	MS.gene93972	0.803903	2.79E-49	-1.69E-46
MS.gene059170	MS.gene93972	0.812144	4.92E-51	-1.69E-46
MS.gene05952	MS.gene93972	0.835416	1.70E-56	-1.69E-46
MS.gene059774	MS.gene93972	0.811525	6.70E-51	-1.69E-46
MS.gene05985	MS.gene93972	-0.80995	1.47E-50	-1.69E-46
MS.gene06023	MS.gene93972	0.844435	7.57E-59	-1.69E-46
MS.gene060259	MS.gene93972	0.842058	3.26E-58	-1.69E-46
MS.gene060412	MS.gene93972	0.828909	6.92E-55	-1.69E-46
MS.gene060413	MS.gene93972	0.822273	2.59E-53	-1.69E-46
MS.gene060697	MS.gene93972	0.808209	3.47E-50	-1.69E-46
MS.gene061047	MS.gene93972	0.817887	2.61E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene93972	MS.gene066130	PPI
MS.gene048677	MS.gene93972	PPI
MS.gene93972	MS.gene45454	PPI
MS.gene034753	MS.gene93972	PPI
MS.gene008306	MS.gene93972	PPI
MS.gene93972	MS.gene064492	PPI
MS.gene41032	MS.gene93972	PPI
MS.gene93972	MS.gene72611	PPI
MS.gene93972	MS.gene048677	PPI
MS.gene04998	MS.gene93972	PPI
MS.gene025667	MS.gene93972	PPI
MS.gene007121	MS.gene93972	PPI
MS.gene061543	MS.gene93972	PPI
MS.gene93972	MS.gene26489	PPI
MS.gene93972	MS.gene066129	PPI
MS.gene93972	MS.gene061546	PPI
MS.gene93972	MS.gene022190	PPI
MS.gene45456	MS.gene93972	PPI
MS.gene45455	MS.gene93972	PPI
MS.gene93972	MS.gene41033	PPI
MS.gene034751	MS.gene93972	PPI
MS.gene93972	MS.gene26139	PPI
MS.gene061546	MS.gene93972	PPI
MS.gene064492	MS.gene93972	PPI
MS.gene017373	MS.gene93972	PPI
MS.gene042912	MS.gene93972	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93972.t1	MTR_5g048050	96.347	438	16	0	1	438	1	438	0.0	867
MS.gene93972.t1	MTR_6g071340	97.608	418	10	0	21	438	1	418	0.0	840
MS.gene93972.t1	MTR_5g077510	48.791	455	203	11	1	438	1	442	3.38e-138	403
MS.gene93972.t1	MTR_3g065080	46.341	451	213	8	1	435	1	438	1.53e-127	376
MS.gene93972.t1	MTR_2g087350	34.901	404	230	11	22	415	3	383	2.29e-55	189
MS.gene93972.t1	MTR_1g056870	31.542	428	246	11	20	415	2	414	5.24e-55	189
MS.gene93972.t1	MTR_1g056880	31.308	428	247	11	20	415	2	414	8.13e-55	188
MS.gene93972.t1	MTR_7g106340	29.782	413	252	11	22	415	5	398	2.07e-50	176
MS.gene93972.t1	MTR_4g085720	32.020	406	242	10	20	416	9	389	2.85e-49	173
MS.gene93972.t1	MTR_1g056840	29.930	431	223	13	20	415	2	388	2.20e-46	165
MS.gene93972.t1	MTR_1g056910	30.348	402	253	11	22	414	3	386	2.13e-42	154
MS.gene93972.t1	MTR_8g092870	26.651	424	280	9	18	429	3	407	4.95e-41	151
MS.gene93972.t1	MTR_5g015210	29.219	397	242	9	25	415	67	430	1.74e-37	142
MS.gene93972.t1	MTR_3g095730	27.358	424	265	15	23	429	11	408	3.16e-36	138
MS.gene93972.t1	MTR_4g051515	27.251	411	261	16	22	415	3	392	1.33e-33	130
MS.gene93972.t1	MTR_4g485520	27.119	413	250	14	18	414	99	476	1.15e-28	118
MS.gene93972.t1	MTR_4g485520	27.119	413	250	14	18	414	267	644	2.30e-28	118
MS.gene93972.t1	MTR_4g063800	27.700	426	234	17	24	422	254	632	1.79e-27	115
MS.gene93972.t1	MTR_1g017770	26.000	400	256	9	25	415	45	413	1.83e-27	114
MS.gene93972.t1	MTR_3g096370	27.670	412	264	14	18	416	3	393	6.22e-26	109
MS.gene93972.t1	MTR_5g083030	25.694	432	250	10	21	417	289	684	3.47e-25	108
MS.gene93972.t1	MTR_7g078330	25.823	395	266	11	23	416	272	640	2.22e-24	106
MS.gene93972.t1	MTR_7g059405	26.536	407	250	16	24	417	263	633	1.31e-23	103
MS.gene93972.t1	MTR_1g094215	27.857	420	245	15	24	425	63	442	1.09e-22	100
MS.gene93972.t1	MTR_4g028960	51.948	77	37	0	20	96	275	351	1.30e-22	101
MS.gene93972.t1	MTR_1g094215	27.857	420	245	15	24	425	246	625	5.47e-22	99.0
MS.gene93972.t1	MTR_3g085610	41.346	104	53	2	11	114	268	363	3.31e-21	97.1
MS.gene93972.t1	MTR_7g053260	34.532	139	76	2	3	141	56	179	7.26e-21	95.1
MS.gene93972.t1	MTR_2g018010	52.857	70	33	0	23	92	290	359	1.29e-20	95.1
MS.gene93972.t1	MTR_3g078160	42.529	87	50	0	10	96	260	346	2.59e-20	94.0
MS.gene93972.t1	MTR_3g078160	42.529	87	50	0	10	96	260	346	2.74e-20	94.0
MS.gene93972.t1	MTR_3g078160	42.529	87	50	0	10	96	260	346	3.29e-20	93.6
MS.gene93972.t1	MTR_4g107010	52.857	70	33	0	26	95	281	350	4.48e-20	93.6
MS.gene93972.t1	MTR_8g080280	24.359	468	275	19	10	420	267	712	4.58e-19	90.5
MS.gene93972.t1	MTR_7g116600	50.617	81	36	2	26	106	79	155	1.02e-18	88.6
MS.gene93972.t1	MTR_5g034440	53.030	66	31	0	27	92	282	347	3.10e-18	87.8
MS.gene93972.t1	MTR_4g091880	47.222	72	37	1	21	91	1	72	2.75e-17	83.2
MS.gene93972.t1	MTR_4g091880	47.222	72	37	1	21	91	1	72	2.95e-17	83.6
MS.gene93972.t1	MTR_1g069845	44.286	70	39	0	23	92	122	191	7.00e-17	83.6
MS.gene93972.t1	MTR_7g097020	46.269	67	36	0	29	95	245	311	8.45e-17	83.6
MS.gene93972.t1	MTR_1g069845	44.286	70	39	0	23	92	243	312	1.19e-16	82.8
MS.gene93972.t1	MTR_1g069845	44.286	70	39	0	23	92	239	308	1.24e-16	82.8
MS.gene93972.t1	MTR_1g079450	39.394	99	53	3	20	118	58	149	5.93e-16	80.1
MS.gene93972.t1	MTR_8g077205	41.860	86	46	2	21	106	159	240	1.81e-15	79.0
MS.gene93972.t1	MTR_8g077205	41.860	86	46	2	21	106	271	352	2.04e-15	79.0
MS.gene93972.t1	MTR_3g466220	38.272	81	50	0	15	95	223	303	4.65e-15	78.2
MS.gene93972.t1	MTR_3g466220	38.272	81	50	0	15	95	202	282	4.93e-15	77.8
MS.gene93972.t1	MTR_5g020570	39.286	84	47	2	23	106	271	350	4.38e-14	75.1
MS.gene93972.t1	MTR_5g020570	39.286	84	47	2	23	106	286	365	5.24e-14	74.7
MS.gene93972.t1	MTR_2g011140	43.750	64	36	0	28	91	18	81	6.58e-14	73.2
MS.gene93972.t1	MTR_7g117890	43.902	82	41	2	11	91	244	321	7.46e-14	74.3
MS.gene93972.t1	MTR_8g103227	46.269	67	35	1	26	91	396	462	5.64e-13	71.6
MS.gene93972.t1	MTR_3g096370	62.791	43	16	0	18	60	3	45	1.01e-12	68.9
MS.gene93972.t1	MTR_2g096850	40.000	70	42	0	22	91	586	655	1.34e-12	70.1
MS.gene93972.t1	MTR_2g096850	40.000	70	42	0	22	91	737	806	1.57e-12	70.1
MS.gene93972.t1	MTR_3g115670	42.105	76	39	1	25	95	30	105	1.67e-12	70.1
MS.gene93972.t1	MTR_1g093965	43.750	64	33	1	38	101	1	61	2.50e-12	66.2
MS.gene93972.t1	MTR_1g090320	41.463	82	39	3	16	95	510	584	3.03e-12	69.3
MS.gene93972.t1	MTR_1g090320	41.463	82	39	3	16	95	512	586	3.22e-12	69.3
MS.gene93972.t1	MTR_7g077780	39.394	66	40	0	26	91	827	892	4.04e-12	68.9
MS.gene93972.t1	MTR_1g093995	43.750	64	33	1	38	101	1	61	5.55e-12	65.1
MS.gene93972.t1	MTR_3g008270	45.455	66	35	1	26	91	731	795	6.75e-12	68.2
MS.gene93972.t1	MTR_5g032010	37.313	67	42	0	25	91	732	798	1.06e-11	67.4
MS.gene93972.t1	MTR_1g100820	37.879	66	41	0	26	91	649	714	1.73e-11	66.6
MS.gene93972.t1	MTR_1g100820	37.879	66	41	0	26	91	647	712	1.79e-11	66.6
MS.gene93972.t1	MTR_7g005940	39.726	73	41	2	25	95	595	666	7.26e-11	65.1
MS.gene93972.t1	MTR_7g005940	39.726	73	41	2	25	95	595	666	7.39e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93972.t1	AT5G37490	43.665	442	230	6	5	438	5	435	6.93e-113	338
MS.gene93972.t1	AT1G66160	41.745	424	220	9	21	438	29	431	5.40e-109	328
MS.gene93972.t1	AT1G66160	39.387	424	214	10	21	438	29	415	2.19e-96	296
MS.gene93972.t1	AT3G02840	39.884	346	195	8	100	438	40	379	1.40e-72	233
MS.gene93972.t1	AT2G35930	33.172	413	238	13	16	415	3	390	2.33e-56	192
MS.gene93972.t1	AT1G49780	31.436	404	256	7	20	416	9	398	8.03e-49	172
MS.gene93972.t1	AT3G19380	30.534	393	246	10	20	403	9	383	5.63e-46	164
MS.gene93972.t1	AT3G11840	29.128	436	250	13	22	414	7	426	1.20e-40	151
MS.gene93972.t1	AT3G52450	45.652	138	67	4	20	155	2	133	3.63e-34	132
MS.gene93972.t1	AT5G09800	27.047	403	258	13	25	416	11	388	6.65e-32	126
MS.gene93972.t1	AT3G49810	27.094	406	253	11	25	422	64	434	2.03e-31	125
MS.gene93972.t1	AT5G64660	27.603	413	269	13	18	416	3	399	1.07e-30	122
MS.gene93972.t1	AT5G65920	27.340	406	252	11	25	422	60	430	1.46e-29	120
MS.gene93972.t1	AT5G65920	27.340	406	252	11	25	422	60	430	1.46e-29	120
MS.gene93972.t1	AT3G18710	25.301	415	260	15	23	416	10	395	6.69e-28	115
MS.gene93972.t1	AT3G54850	27.561	410	246	14	24	417	247	621	9.77e-26	110
MS.gene93972.t1	AT2G28830	27.518	407	248	14	23	413	182	557	5.28e-25	108
MS.gene93972.t1	AT1G71020	26.014	419	266	14	12	416	82	470	7.14e-25	107
MS.gene93972.t1	AT1G71020	26.014	419	266	14	12	416	230	618	1.48e-24	107
MS.gene93972.t1	AT1G23030	27.644	416	254	16	12	416	228	607	1.97e-24	106
MS.gene93972.t1	AT3G46510	25.176	425	271	16	3	414	235	625	1.98e-22	100
MS.gene93972.t1	AT5G42340	26.984	252	148	5	14	232	279	527	3.41e-21	96.7
MS.gene93972.t1	AT5G01830	51.389	72	35	0	25	96	274	345	3.05e-20	94.0
MS.gene93972.t1	AT1G29340	50.704	71	35	0	23	93	303	373	2.75e-19	91.3
MS.gene93972.t1	AT1G24330	33.533	167	90	6	2	153	246	406	1.10e-18	89.4
MS.gene93972.t1	AT1G24330	33.533	167	90	6	2	153	246	406	1.10e-18	89.4
MS.gene93972.t1	AT1G27910	38.596	114	62	3	14	125	268	375	1.21e-18	89.0
MS.gene93972.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.04e-18	88.2
MS.gene93972.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.05e-18	88.6
MS.gene93972.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.05e-18	88.6
MS.gene93972.t1	AT1G67530	35.606	132	75	3	2	127	243	370	2.05e-18	88.6
MS.gene93972.t1	AT3G07360	45.570	79	40	1	23	101	72	147	1.04e-17	85.5
MS.gene93972.t1	AT1G10560	32.414	145	84	2	31	164	294	435	2.01e-17	85.1
MS.gene93972.t1	AT3G54790	43.421	76	43	0	23	98	200	275	9.59e-17	83.2
MS.gene93972.t1	AT1G01680	37.931	87	49	1	9	95	222	303	1.12e-16	80.9
MS.gene93972.t1	AT3G54790	43.421	76	43	0	23	98	236	311	1.30e-16	82.8
MS.gene93972.t1	AT3G54790	43.421	76	43	0	23	98	236	311	1.30e-16	82.8
MS.gene93972.t1	AT4G21350	47.222	72	37	1	21	91	1	72	3.25e-16	80.5
MS.gene93972.t1	AT1G60190	32.215	149	83	4	27	164	280	421	2.82e-15	78.6
MS.gene93972.t1	AT4G36550	37.500	72	45	0	24	95	218	289	1.81e-14	76.3
MS.gene93972.t1	AT3G61390	43.939	66	37	0	26	91	279	344	3.76e-14	73.9
MS.gene93972.t1	AT3G61390	43.939	66	37	0	26	91	354	419	5.17e-14	73.9
MS.gene93972.t1	AT2G23140	36.082	97	58	1	23	119	228	320	1.39e-13	73.6
MS.gene93972.t1	AT2G23140	36.082	97	58	1	23	119	228	320	1.39e-13	73.6
MS.gene93972.t1	AT2G23140	36.082	97	58	1	23	119	231	323	1.42e-13	73.6
MS.gene93972.t1	AT3G49060	42.424	66	38	0	26	91	736	801	1.65e-13	73.2
MS.gene93972.t1	AT3G49060	42.424	66	38	0	26	91	703	768	1.66e-13	73.2
MS.gene93972.t1	AT3G49060	42.424	66	38	0	26	91	726	791	1.67e-13	73.2
MS.gene93972.t1	AT2G23140	36.082	97	58	1	23	119	39	131	1.74e-13	72.8
MS.gene93972.t1	AT2G23140	36.082	97	58	1	23	119	39	131	1.74e-13	72.8
MS.gene93972.t1	AT1G01660	38.571	70	43	0	26	95	499	568	1.82e-13	72.8
MS.gene93972.t1	AT1G01660	38.571	70	43	0	26	95	498	567	1.85e-13	72.8
MS.gene93972.t1	AT2G45920	42.424	66	38	0	26	91	326	391	2.14e-13	72.0
MS.gene93972.t1	AT1G56040	38.824	85	51	1	7	90	334	418	1.32e-12	69.7
MS.gene93972.t1	AT5G67340	36.986	73	46	0	23	95	238	310	1.52e-12	70.1
MS.gene93972.t1	AT5G67340	36.986	73	46	0	23	95	238	310	1.52e-12	70.1
MS.gene93972.t1	AT1G56030	43.077	65	37	0	26	90	324	388	2.01e-12	68.9
MS.gene93972.t1	AT5G65500	41.333	75	41	1	17	91	718	789	2.22e-12	69.7
MS.gene93972.t1	AT5G51270	40.845	71	41	1	26	96	736	805	2.28e-12	69.7
MS.gene93972.t1	AT5G51270	40.845	71	41	1	26	96	736	805	2.28e-12	69.7
MS.gene93972.t1	AT5G65500	41.333	75	41	1	17	91	710	781	2.35e-12	69.7
MS.gene93972.t1	AT1G01670	40.909	66	39	0	26	91	211	276	1.12e-11	65.5
MS.gene93972.t1	AT1G01670	40.909	66	39	0	26	91	295	360	1.94e-11	65.9
MS.gene93972.t1	AT1G01670	40.909	66	39	0	26	91	295	360	1.94e-11	65.9
MS.gene93972.t1	AT5G18340	32.110	109	67	3	12	120	61	162	4.83e-11	65.1
MS.gene93972.t1	AT5G18320	30.709	127	82	4	23	146	70	193	6.22e-11	64.7

Find 53 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGAAAGCTTTAACTATTT+TGG	0.084598	6.2:-73813538	MS.gene93972:CDS
TCAAATTGATGAATCCTTAT+AGG	0.188148	6.2:-73813248	MS.gene93972:CDS
AATCATCATTGAGGATAATT+TGG	0.200592	6.2:-73813674	MS.gene93972:CDS
TTCATCAAATCAAGAGTAAT+TGG	0.206017	6.2:+73814392	None:intergenic
AGTATTCAGTTTCTACTATA+TGG	0.256281	6.2:-73813410	MS.gene93972:CDS
CTTCTTTACATTGCATGATT+TGG	0.259797	6.2:-73813863	MS.gene93972:CDS
CAAATTGATGAATCCTTATA+GGG	0.287604	6.2:-73813247	MS.gene93972:CDS
TGATTCCAAATCATTCTCTT+AGG	0.293043	6.2:-73814253	MS.gene93972:CDS
TTGAAAGAGATTCTTTCTTT+TGG	0.293237	6.2:-73813792	MS.gene93972:CDS
TGACTTAACAATTCCTTTGT+TGG	0.326123	6.2:-73813469	MS.gene93972:CDS
ATCGAGCTAACCGGTATTCT+TGG	0.327497	6.2:+73814170	None:intergenic
AAGTTCAGTTGCTTTATCTT+TGG	0.334075	6.2:+73813272	None:intergenic
TCTTCCAATTCAGCCCTATA+AGG	0.335630	6.2:+73813234	None:intergenic
ATTGGACACCTAAATTGATT+TGG	0.335859	6.2:+73814410	None:intergenic
GGTATCACTACCTCCATGTT+TGG	0.338704	6.2:+73814431	None:intergenic
GAAACATGTAGAAGCACTTA+TGG	0.341465	6.2:-73813763	MS.gene93972:CDS
GTTTAAGATGGTGTTAGGTT+GGG	0.345500	6.2:-73814492	None:intergenic
TAGTGATACCAAATCAATTT+AGG	0.346434	6.2:-73814418	MS.gene93972:CDS
GAATCCTTATAGGGCTGAAT+TGG	0.347326	6.2:-73813238	MS.gene93972:CDS
TTTGAAGCATCAAGATGTTA+AGG	0.349669	6.2:-73813838	MS.gene93972:CDS
TGTTTAAGATGGTGTTAGGT+TGG	0.352531	6.2:-73814493	None:intergenic
AAAGTGTTTAAGATGGTGTT+AGG	0.352803	6.2:-73814497	None:intergenic
GCAAAAGGTTTGGATCAATA+TGG	0.357148	6.2:-73814110	MS.gene93972:CDS
ATATGGAAGATGTGCAAATA+TGG	0.363736	6.2:-73813393	MS.gene93972:CDS
TTCATTTGTAGAATTGGGTT+TGG	0.379894	6.2:-73813607	MS.gene93972:CDS
TTCAATACTCTCTCTATCAT+AGG	0.380174	6.2:+73814340	None:intergenic
TGGTGTTAGGTTGGGGAAGA+AGG	0.382817	6.2:-73814484	MS.gene93972:CDS
ATTATCCTAAGAGAATGATT+TGG	0.383748	6.2:+73814248	None:intergenic
TCAATATGGTTGTATTGAAT+TGG	0.405007	6.2:-73814096	MS.gene93972:CDS
TAAGGAATCAGCAAAAGGTT+TGG	0.411626	6.2:-73814120	MS.gene93972:CDS
TTGAAGCATCAAGATGTTAA+GGG	0.415181	6.2:-73813837	MS.gene93972:CDS
TTAGGATAATGATTCAAGAT+TGG	0.432186	6.2:-73814235	MS.gene93972:CDS
GAAACATCGATCGAGCTAAC+CGG	0.450457	6.2:+73814161	None:intergenic
CGAGTTAAGGAATCAGCAAA+AGG	0.455195	6.2:-73814125	MS.gene93972:CDS
GATCCTGTGACATTGTCAAC+AGG	0.477744	6.2:-73814368	MS.gene93972:CDS
TTTAAGATGGTGTTAGGTTG+GGG	0.480803	6.2:-73814491	None:intergenic
TGTGTTGAGAATAGACAAAA+TGG	0.498744	6.2:-73814212	MS.gene93972:CDS
CTATTATTGGTATTGCAAGT+TGG	0.507419	6.2:-73813309	MS.gene93972:CDS
ATTGAAAGATGGTTCAATGA+AGG	0.518770	6.2:-73814323	MS.gene93972:CDS
ATAGAGAGAGTATTGAAAGA+TGG	0.521682	6.2:-73814334	MS.gene93972:CDS
GAAGCACTTATGGAAATTGA+AGG	0.535964	6.2:-73813753	MS.gene93972:CDS
ATTCCTGTTGACAATGTCAC+AGG	0.541677	6.2:+73814365	None:intergenic
CAAAGGTGGAAAACCAAACA+TGG	0.558197	6.2:-73814444	MS.gene93972:CDS
TTGATTGAAGCACTTCAAGT+TGG	0.561935	6.2:-73813345	MS.gene93972:CDS
GATTCCTACTCCAAGAATAC+CGG	0.564162	6.2:-73814180	MS.gene93972:CDS
ACTGAATACTCTGTGGTCAA+AGG	0.567201	6.2:+73813423	None:intergenic
CTTACAACTTGATTTGTGAG+AGG	0.581086	6.2:+73814287	None:intergenic
TAATCACAAAATCATCATTG+AGG	0.586969	6.2:-73813683	MS.gene93972:CDS
CTAACCGGTATTCTTGGAGT+AGG	0.590630	6.2:+73814176	None:intergenic
AATGGAGTAATGAAAGTGAG+AGG	0.609225	6.2:-73814058	MS.gene93972:CDS
ATAGACAAAATGGTGTTGAG+AGG	0.615873	6.2:-73814202	MS.gene93972:CDS
AGTAGAAACTGAATACTCTG+TGG	0.711233	6.2:+73813416	None:intergenic
AGGTGGAAAACCAAACATGG+AGG	0.758131	6.2:-73814441	MS.gene93972:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTCATTTGTAGAATT+GGG	-	chr6.2:73814064-73814083	MS.gene93972:CDS	15.0%
!!!	TTTTTCATTGAAATTAGAAT+TGG	+	chr6.2:73814041-73814060	None:intergenic	15.0%
!!!	AATGATGATTTTGTGATTAT+TGG	+	chr6.2:73813992-73814011	None:intergenic	20.0%
!!!	GAAATTTTCATTTGTAGAAT+TGG	-	chr6.2:73814063-73814082	MS.gene93972:CDS	20.0%
!!!	TATTGAATTGGTTCAAAAAA+TGG	-	chr6.2:73813592-73813611	MS.gene93972:CDS	20.0%
!	AAAAAATGCATAGTTGAGAA+TGG	-	chr6.2:73813644-73813663	MS.gene93972:CDS	25.0%
!	AAGAACAAGAAAAACAACAA+AGG	-	chr6.2:73813215-73813234	MS.gene93972:CDS	25.0%
!	AATCATCATTGAGGATAATT+TGG	-	chr6.2:73814002-73814021	MS.gene93972:CDS	25.0%
!	AGTATTCAGTTTCTACTATA+TGG	-	chr6.2:73814266-73814285	MS.gene93972:CDS	25.0%
!	ATTATCCTAAGAGAATGATT+TGG	+	chr6.2:73813431-73813450	None:intergenic	25.0%
!	CAAATTGATGAATCCTTATA+GGG	-	chr6.2:73814429-73814448	MS.gene93972:CDS	25.0%
!	TAATCACAAAATCATCATTG+AGG	-	chr6.2:73813993-73814012	MS.gene93972:CDS	25.0%
!	TAGTGATACCAAATCAATTT+AGG	-	chr6.2:73813258-73813277	MS.gene93972:CDS	25.0%
!	TATGGTCACAAAAATTATGA+AGG	-	chr6.2:73814301-73814320	MS.gene93972:CDS	25.0%
!	TCAAATTGATGAATCCTTAT+AGG	-	chr6.2:73814428-73814447	MS.gene93972:CDS	25.0%
!	TCAATATGGTTGTATTGAAT+TGG	-	chr6.2:73813580-73813599	MS.gene93972:CDS	25.0%
!	TTAGAAGCTCAAAAATCTTT+AGG	-	chr6.2:73813782-73813801	MS.gene93972:CDS	25.0%
!	TTAGGATAATGATTCAAGAT+TGG	-	chr6.2:73813441-73813460	MS.gene93972:CDS	25.0%
!	TTCATCAAATCAAGAGTAAT+TGG	+	chr6.2:73813287-73813306	None:intergenic	25.0%
!!	TGATTTTATCAGCACTTAAT+TGG	-	chr6.2:73813744-73813763	MS.gene93972:CDS	25.0%
!!	TTGAAAGAGATTCTTTCTTT+TGG	-	chr6.2:73813884-73813903	MS.gene93972:CDS	25.0%
!!!	AAGATTTTTTTGACCAACAA+AGG	+	chr6.2:73814223-73814242	None:intergenic	25.0%
!!!	TGAGAAAGCTTTAACTATTT+TGG	-	chr6.2:73814138-73814157	MS.gene93972:CDS	25.0%
	AACAAGAAAAACAACAAAGG+TGG	-	chr6.2:73813218-73813237	MS.gene93972:CDS	30.0%
	ATATGGAAGATGTGCAAATA+TGG	-	chr6.2:73814283-73814302	MS.gene93972:CDS	30.0%
	ATTGAAAGATGGTTCAATGA+AGG	-	chr6.2:73813353-73813372	MS.gene93972:CDS	30.0%
	ATTGGACACCTAAATTGATT+TGG	+	chr6.2:73813269-73813288	None:intergenic	30.0%
	CTATTATTGGTATTGCAAGT+TGG	-	chr6.2:73814367-73814386	MS.gene93972:CDS	30.0%
	CTTCTTTACATTGCATGATT+TGG	-	chr6.2:73813813-73813832	MS.gene93972:CDS	30.0%
	TGACTTAACAATTCCTTTGT+TGG	-	chr6.2:73814207-73814226	MS.gene93972:CDS	30.0%
	TGATTCCAAATCATTCTCTT+AGG	-	chr6.2:73813423-73813442	MS.gene93972:CDS	30.0%
	TGGTTCAAAAAATGGAGAAA+TGG	-	chr6.2:73813600-73813619	MS.gene93972:CDS	30.0%
	TGTGTTGAGAATAGACAAAA+TGG	-	chr6.2:73813464-73813483	MS.gene93972:CDS	30.0%
	TTCAATACTCTCTCTATCAT+AGG	+	chr6.2:73813339-73813358	None:intergenic	30.0%
	TTCATTTGTAGAATTGGGTT+TGG	-	chr6.2:73814069-73814088	MS.gene93972:CDS	30.0%
!	AAGTTCAGTTGCTTTATCTT+TGG	+	chr6.2:73814407-73814426	None:intergenic	30.0%
!	TGCTTTTCAGAAGCTATTAT+TGG	-	chr6.2:73814354-73814373	MS.gene93972:CDS	30.0%
!	TTGAAGCATCAAGATGTTAA+GGG	-	chr6.2:73813839-73813858	MS.gene93972:CDS	30.0%
!	TTTGAAGCATCAAGATGTTA+AGG	-	chr6.2:73813838-73813857	MS.gene93972:CDS	30.0%
!	TTTTGAGCTTCTAATTGAAG+TGG	+	chr6.2:73813776-73813795	None:intergenic	30.0%
!!	ATAGAGAGAGTATTGAAAGA+TGG	-	chr6.2:73813342-73813361	MS.gene93972:CDS	30.0%
	AATGGAGTAATGAAAGTGAG+AGG	-	chr6.2:73813618-73813637	MS.gene93972:CDS	35.0%
	AGTAGAAACTGAATACTCTG+TGG	+	chr6.2:73814263-73814282	None:intergenic	35.0%
	CTTAACTCGAAAAAGAAGCT+CGG	+	chr6.2:73813540-73813559	None:intergenic	35.0%
	GCAAAAGGTTTGGATCAATA+TGG	-	chr6.2:73813566-73813585	MS.gene93972:CDS	35.0%
	TAAGGAATCAGCAAAAGGTT+TGG	-	chr6.2:73813556-73813575	MS.gene93972:CDS	35.0%
!	ATAGACAAAATGGTGTTGAG+AGG	-	chr6.2:73813474-73813493	MS.gene93972:CDS	35.0%
!	GAAGCACTTATGGAAATTGA+AGG	-	chr6.2:73813923-73813942	MS.gene93972:CDS	35.0%
!	TTGATTGAAGCACTTCAAGT+TGG	-	chr6.2:73814331-73814350	MS.gene93972:CDS	35.0%
!!	CTTACAACTTGATTTGTGAG+AGG	+	chr6.2:73813392-73813411	None:intergenic	35.0%
!!	GAAACATGTAGAAGCACTTA+TGG	-	chr6.2:73813913-73813932	MS.gene93972:CDS	35.0%
	ACTGAATACTCTGTGGTCAA+AGG	+	chr6.2:73814256-73814275	None:intergenic	40.0%
	ATTCCTGTTGACAATGTCAC+AGG	+	chr6.2:73813314-73813333	None:intergenic	40.0%
	CAAAGGTGGAAAACCAAACA+TGG	-	chr6.2:73813232-73813251	MS.gene93972:CDS	40.0%
	CGAGTTAAGGAATCAGCAAA+AGG	-	chr6.2:73813551-73813570	MS.gene93972:CDS	40.0%
	GAATCCTTATAGGGCTGAAT+TGG	-	chr6.2:73814438-73814457	MS.gene93972:CDS	40.0%
	GATTCCTACTCCAAGAATAC+CGG	-	chr6.2:73813496-73813515	MS.gene93972:CDS	40.0%
	TCTTCCAATTCAGCCCTATA+AGG	+	chr6.2:73814445-73814464	None:intergenic	40.0%
!!!	CGAGCTTCTTTTTCGAGTTA+AGG	-	chr6.2:73813538-73813557	MS.gene93972:CDS	40.0%
	AGGTGGAAAACCAAACATGG+AGG	-	chr6.2:73813235-73813254	MS.gene93972:CDS	45.0%
	ATCGAGCTAACCGGTATTCT+TGG	+	chr6.2:73813509-73813528	None:intergenic	45.0%
	CTAACCGGTATTCTTGGAGT+AGG	+	chr6.2:73813503-73813522	None:intergenic	45.0%
	GAAACATCGATCGAGCTAAC+CGG	+	chr6.2:73813518-73813537	None:intergenic	45.0%
	GATCCTGTGACATTGTCAAC+AGG	-	chr6.2:73813308-73813327	MS.gene93972:CDS	45.0%
	GGTATCACTACCTCCATGTT+TGG	+	chr6.2:73813248-73813267	None:intergenic	45.0%
!!	TGGTGTTAGGTTGGGGAAGA+AGG	-	chr6.2:73813192-73813211	MS.gene93972:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	73813191	73814507	73813191	ID=MS.gene93972
chr6.2	mRNA	73813191	73814507	73813191	ID=MS.gene93972.t1;Parent=MS.gene93972
chr6.2	exon	73813191	73814507	73813191	ID=MS.gene93972.t1.exon1;Parent=MS.gene93972.t1
chr6.2	CDS	73813191	73814507	73813191	ID=cds.MS.gene93972.t1;Parent=MS.gene93972.t1

Gene Sequence

>MS.gene93972

ATGGTGTTAGGTTGGGGAAGAAGGAAGAACAAGAAAAACAACAAAGGTGGAAAACCAAACATGGAGGTAGTGATACCAAATCAATTTAGGTGTCCAATTACTCTTGATTTGATGAAAGATCCTGTGACATTGTCAACAGGAATAACCTATGATAGAGAGAGTATTGAAAGATGGTTCAATGAAGGAAACTATACATGTCCTCTCACAAATCAAGTTGTAAGAAACTTTGATATGATTCCAAATCATTCTCTTAGGATAATGATTCAAGATTGGTGTGTTGAGAATAGACAAAATGGTGTTGAGAGGATTCCTACTCCAAGAATACCGGTTAGCTCGATCGATGTTTCCGAGCTTCTTTTTCGAGTTAAGGAATCAGCAAAAGGTTTGGATCAATATGGTTGTATTGAATTGGTTCAAAAAATGGAGAAATGGAGTAATGAAAGTGAGAGGAACAAAAAATGCATAGTTGAGAATGGAGCAACTAGTGCATTAGCTTTAGCATTTGATGCTTTTGCTAATGATTCTATTGAGAAAAATGTTATTGTTCTTGAAGTGATTTTATCAGCACTTAATTGGATGTTTCCACTTCAATTAGAAGCTCAAAAATCTTTAGGTTCAAAAGCTTCTTTACATTGCATGATTTGGTTTTTGAAGCATCAAGATGTTAAGGGAAAAGAAAAAGCTATTATTGCATTGAAAGAGATTCTTTCTTTTGGTGATGAGAAACATGTAGAAGCACTTATGGAAATTGAAGGAGTTAATGAGCTTTTAATTGAGTTCATTAACAAGAGAATTAGTCCAATAATCACAAAATCATCATTGAGGATAATTTGGTACTTAGTTTCATCCAATTCTAATTTCAATGAAAAAATGAAATTTTCATTTGTAGAATTGGGTTTGGTTTCTTCAATTTTGCACATTCTTATTGACTCAGAAAAAAGCACTTGTGAGAAAGCTTTAACTATTTTGGATTCTTTATTAAGCTGTGATTTTGCAAGAGAAACAGCTTATGAAAATGACTTAACAATTCCTTTGTTGGTCAAAAAAATCTTGAGAGTTTCACCTTTGACCACAGAGTATTCAGTTTCTACTATATGGAAGATGTGCAAATATGGTCACAAAAATTATGAAGGAAAAGCTTTGATTGAAGCACTTCAAGTTGGTGCTTTTCAGAAGCTATTATTGGTATTGCAAGTTGGATGTAATGATGAGACCAAAGATAAAGCAACTGAACTTCTCAAATTGATGAATCCTTATAGGGCTGAATTGGAAGATTGTGTTGATTCAGATTTCAAGAATCTTAAGAGATCATATTAA

Protein sequence

>MS.gene93972.t1

MVLGWGRRKNKKNNKGGKPNMEVVIPNQFRCPITLDLMKDPVTLSTGITYDRESIERWFNEGNYTCPLTNQVVRNFDMIPNHSLRIMIQDWCVENRQNGVERIPTPRIPVSSIDVSELLFRVKESAKGLDQYGCIELVQKMEKWSNESERNKKCIVENGATSALALAFDAFANDSIEKNVIVLEVILSALNWMFPLQLEAQKSLGSKASLHCMIWFLKHQDVKGKEKAIIALKEILSFGDEKHVEALMEIEGVNELLIEFINKRISPIITKSSLRIIWYLVSSNSNFNEKMKFSFVELGLVSSILHILIDSEKSTCEKALTILDSLLSCDFARETAYENDLTIPLLVKKILRVSPLTTEYSVSTIWKMCKYGHKNYEGKALIEALQVGAFQKLLLVLQVGCNDETKDKATELLKLMNPYRAELEDCVDSDFKNLKRSY