AlfalfaGEDB | MS.gene064492

M. sativa cultivar XinJiangDaYe / MS.gene064492

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064492.t1	XP_003616222.1	97.1	442	13	0	1	442	1	442	5.20E-239	836.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064492.t1	Q9C8D1	44.5	434	212	9	19	442	17	431	1.4e-96	354.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064492.t1	G7KFZ3	97.1	442	13	0	1	442	1	442	3.7e-239	836.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055549	MS.gene064492	0.806139	9.51E-50	-1.69E-46
MS.gene055550	MS.gene064492	0.810269	1.25E-50	-1.69E-46
MS.gene055551	MS.gene064492	0.814632	1.40E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene064492	MS.gene45454	PPI
MS.gene45455	MS.gene064492	PPI
MS.gene061546	MS.gene064492	PPI
MS.gene017373	MS.gene064492	PPI
MS.gene064492	MS.gene26139	PPI
MS.gene042912	MS.gene064492	PPI
MS.gene064492	MS.gene72611	PPI
MS.gene93972	MS.gene064492	PPI
MS.gene064492	MS.gene048677	PPI
MS.gene034751	MS.gene064492	PPI
MS.gene034753	MS.gene064492	PPI
MS.gene41032	MS.gene064492	PPI
MS.gene064492	MS.gene26489	PPI
MS.gene064492	MS.gene066129	PPI
MS.gene064492	MS.gene061546	PPI
MS.gene064492	MS.gene022190	PPI
MS.gene04998	MS.gene064492	PPI
MS.gene048677	MS.gene064492	PPI
MS.gene025667	MS.gene064492	PPI
MS.gene008306	MS.gene064492	PPI
MS.gene007121	MS.gene064492	PPI
MS.gene061543	MS.gene064492	PPI
MS.gene45456	MS.gene064492	PPI
MS.gene064492	MS.gene41033	PPI
MS.gene064492	MS.gene93972	PPI
MS.gene064492	MS.gene066130	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064492.t1	MTR_5g077510	97.059	442	13	0	1	442	1	442	0.0	873
MS.gene064492.t1	MTR_3g065080	64.334	443	149	8	1	439	1	438	0.0	523
MS.gene064492.t1	MTR_6g071340	48.471	425	199	8	31	442	1	418	4.37e-129	379
MS.gene064492.t1	MTR_5g048050	48.122	426	201	8	30	442	20	438	1.16e-126	374
MS.gene064492.t1	MTR_4g085720	34.414	401	233	11	29	418	8	389	6.32e-60	201
MS.gene064492.t1	MTR_2g087350	35.452	409	235	12	32	428	3	394	6.95e-56	190
MS.gene064492.t1	MTR_7g106340	33.333	426	244	13	32	433	5	414	7.79e-56	191
MS.gene064492.t1	MTR_1g056880	33.487	433	241	15	32	428	4	425	5.90e-52	181
MS.gene064492.t1	MTR_1g056870	33.638	437	235	16	32	428	4	425	1.00e-51	180
MS.gene064492.t1	MTR_1g056910	31.159	414	260	11	32	433	3	403	2.33e-51	178
MS.gene064492.t1	MTR_1g056840	31.724	435	220	16	32	428	4	399	1.17e-46	166
MS.gene064492.t1	MTR_4g051515	27.381	420	265	14	32	429	3	404	2.54e-35	135
MS.gene064492.t1	MTR_8g092870	28.341	434	264	19	33	442	8	418	6.22e-35	134
MS.gene064492.t1	MTR_3g095730	26.353	425	282	18	33	442	11	419	3.96e-34	132
MS.gene064492.t1	MTR_5g015210	30.326	399	227	12	35	417	67	430	3.02e-33	130
MS.gene064492.t1	MTR_4g063800	27.586	406	242	16	34	419	254	627	5.51e-32	129
MS.gene064492.t1	MTR_1g017770	28.827	392	247	9	35	417	45	413	3.26e-30	121
MS.gene064492.t1	MTR_4g107010	28.271	428	266	15	15	425	267	670	2.25e-27	115
MS.gene064492.t1	MTR_4g485520	26.087	414	266	15	22	418	88	478	2.43e-27	114
MS.gene064492.t1	MTR_4g485520	25.815	399	260	14	33	418	271	646	3.88e-27	114
MS.gene064492.t1	MTR_3g096370	60.000	75	30	0	27	101	2	76	8.22e-26	108
MS.gene064492.t1	MTR_5g083030	25.121	414	263	11	33	419	291	684	1.15e-25	110
MS.gene064492.t1	MTR_7g059405	26.034	411	237	18	35	419	264	633	1.66e-24	106
MS.gene064492.t1	MTR_7g078330	24.060	399	260	14	33	418	272	640	2.77e-24	106
MS.gene064492.t1	MTR_2g018010	26.349	315	194	9	33	329	290	584	1.82e-23	103
MS.gene064492.t1	MTR_5g034440	25.647	425	279	11	15	424	268	670	7.03e-22	99.0
MS.gene064492.t1	MTR_4g028960	46.053	76	41	0	31	106	276	351	1.82e-21	97.8
MS.gene064492.t1	MTR_3g078160	26.969	419	268	16	24	424	264	662	2.69e-21	97.1
MS.gene064492.t1	MTR_3g078160	26.969	419	268	16	24	424	264	662	2.76e-21	97.1
MS.gene064492.t1	MTR_3g078160	34.532	139	84	3	24	162	264	395	2.63e-20	94.0
MS.gene064492.t1	MTR_1g094215	26.531	392	260	11	35	418	64	435	3.25e-20	92.8
MS.gene064492.t1	MTR_1g094215	26.718	393	258	12	35	418	247	618	7.85e-20	92.4
MS.gene064492.t1	MTR_4g091880	24.490	392	262	12	35	420	5	368	1.79e-19	90.1
MS.gene064492.t1	MTR_3g085610	41.667	84	43	1	36	119	281	358	3.98e-19	90.5
MS.gene064492.t1	MTR_4g091880	48.529	68	34	1	35	101	5	72	1.26e-18	87.0
MS.gene064492.t1	MTR_1g069845	44.286	70	39	0	33	102	122	191	3.43e-18	87.4
MS.gene064492.t1	MTR_7g097020	37.805	82	51	0	21	102	227	308	4.26e-18	87.4
MS.gene064492.t1	MTR_1g069845	44.286	70	39	0	33	102	239	308	6.20e-18	87.0
MS.gene064492.t1	MTR_1g069845	44.286	70	39	0	33	102	243	312	6.27e-18	87.0
MS.gene064492.t1	MTR_7g116600	29.667	300	178	12	32	316	75	356	1.63e-17	84.7
MS.gene064492.t1	MTR_7g053260	43.478	69	39	0	33	101	76	144	1.32e-16	82.0
MS.gene064492.t1	MTR_2g011140	39.506	81	49	0	21	101	1	81	2.27e-16	80.9
MS.gene064492.t1	MTR_1g079450	26.821	302	188	12	30	316	58	341	3.12e-16	80.9
MS.gene064492.t1	MTR_2g096850	42.857	70	40	0	32	101	737	806	2.12e-15	79.0
MS.gene064492.t1	MTR_8g080280	38.095	84	46	1	36	119	283	360	2.17e-15	79.0
MS.gene064492.t1	MTR_2g096850	42.857	70	40	0	32	101	586	655	2.33e-15	79.0
MS.gene064492.t1	MTR_8g077205	41.667	72	42	0	31	102	159	230	6.36e-15	77.4
MS.gene064492.t1	MTR_8g077205	41.667	72	42	0	31	102	271	342	6.80e-15	77.4
MS.gene064492.t1	MTR_7g077780	40.000	70	42	0	36	105	827	896	1.76e-14	76.3
MS.gene064492.t1	MTR_7g117890	24.138	232	144	4	39	265	259	463	2.99e-14	75.5
MS.gene064492.t1	MTR_3g115670	31.333	150	80	4	39	183	34	165	4.14e-14	75.1
MS.gene064492.t1	MTR_5g032010	33.333	87	58	0	15	101	712	798	5.24e-14	74.7
MS.gene064492.t1	MTR_3g096370	64.444	45	16	0	27	71	2	46	1.54e-13	71.2
MS.gene064492.t1	MTR_1g100820	39.394	66	40	0	36	101	649	714	1.90e-13	72.8
MS.gene064492.t1	MTR_5g020570	38.095	84	48	2	33	116	271	350	1.92e-13	72.8
MS.gene064492.t1	MTR_1g100820	39.394	66	40	0	36	101	647	712	1.96e-13	72.8
MS.gene064492.t1	MTR_5g020570	38.095	84	48	2	33	116	286	365	2.15e-13	72.8
MS.gene064492.t1	MTR_1g090320	41.860	86	45	3	36	117	514	598	2.44e-13	72.8
MS.gene064492.t1	MTR_1g090320	41.860	86	45	3	36	117	516	600	2.53e-13	72.8
MS.gene064492.t1	MTR_3g466220	37.143	70	44	0	33	102	231	300	5.47e-13	71.6
MS.gene064492.t1	MTR_3g466220	37.143	70	44	0	33	102	210	279	5.95e-13	71.6
MS.gene064492.t1	MTR_3g008270	43.939	66	36	1	36	101	731	795	1.98e-12	69.7
MS.gene064492.t1	MTR_8g103227	43.284	67	37	1	36	101	396	462	3.32e-12	69.3
MS.gene064492.t1	MTR_7g005940	42.857	70	37	2	34	101	594	662	3.69e-12	68.9
MS.gene064492.t1	MTR_7g005940	42.857	70	37	2	34	101	594	662	3.75e-12	68.9
MS.gene064492.t1	MTR_1g093965	45.312	64	32	1	48	111	1	61	5.42e-12	65.1
MS.gene064492.t1	MTR_1g093995	45.312	64	32	1	48	111	1	61	6.62e-12	64.7
MS.gene064492.t1	MTR_8g027140	23.707	232	145	6	39	265	263	467	8.40e-12	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064492.t1	AT1G66160	44.719	445	215	11	10	442	6	431	3.42e-124	367
MS.gene064492.t1	AT5G37490	45.215	418	212	8	32	442	28	435	7.19e-113	339
MS.gene064492.t1	AT1G66160	42.472	445	209	12	10	442	6	415	3.21e-111	334
MS.gene064492.t1	AT3G02840	41.504	359	180	11	103	442	32	379	1.86e-71	230
MS.gene064492.t1	AT3G19380	32.992	391	233	10	29	405	8	383	6.01e-60	201
MS.gene064492.t1	AT2G35930	34.826	402	226	12	32	417	9	390	9.32e-60	201
MS.gene064492.t1	AT1G49780	33.831	402	243	10	29	418	8	398	1.09e-56	193
MS.gene064492.t1	AT3G52450	31.264	435	260	10	32	434	4	431	2.56e-53	184
MS.gene064492.t1	AT3G11840	31.066	441	253	13	32	429	7	439	2.95e-43	158
MS.gene064492.t1	AT3G49810	28.788	396	260	6	28	423	60	433	8.90e-35	134
MS.gene064492.t1	AT5G64660	27.465	426	280	17	33	442	8	420	1.04e-33	131
MS.gene064492.t1	AT5G65920	27.621	391	264	4	35	425	60	431	1.42e-33	131
MS.gene064492.t1	AT5G65920	27.621	391	264	4	35	425	60	431	1.42e-33	131
MS.gene064492.t1	AT5G09800	26.781	407	261	13	27	418	4	388	9.16e-32	125
MS.gene064492.t1	AT2G28830	25.616	406	261	15	36	424	185	566	7.29e-28	116
MS.gene064492.t1	AT1G23030	26.952	397	253	13	32	418	238	607	2.55e-27	115
MS.gene064492.t1	AT3G18710	25.850	441	273	14	27	442	4	415	6.04e-26	109
MS.gene064492.t1	AT1G71020	27.696	408	243	16	33	418	93	470	8.99e-26	109
MS.gene064492.t1	AT3G46510	26.263	396	252	15	35	416	256	625	1.34e-25	110
MS.gene064492.t1	AT1G71020	27.696	408	243	16	33	418	241	618	1.63e-25	109
MS.gene064492.t1	AT3G54850	26.076	395	259	12	35	418	248	620	2.32e-23	103
MS.gene064492.t1	AT1G10560	25.799	407	258	11	41	424	294	679	2.92e-23	103
MS.gene064492.t1	AT5G42340	30.625	160	106	2	35	193	290	445	3.15e-22	100
MS.gene064492.t1	AT5G01830	52.941	68	32	0	35	102	274	341	5.15e-19	90.1
MS.gene064492.t1	AT1G29340	35.811	148	84	4	33	170	303	449	6.73e-19	90.1
MS.gene064492.t1	AT1G60190	22.807	399	283	9	39	424	282	668	9.81e-19	89.4
MS.gene064492.t1	AT4G21350	27.508	309	195	10	35	338	5	289	1.07e-18	87.8
MS.gene064492.t1	AT1G27910	38.235	102	51	2	36	128	280	378	1.07e-16	83.2
MS.gene064492.t1	AT3G54790	43.243	74	42	0	33	106	200	273	1.09e-16	83.2
MS.gene064492.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.46e-16	82.8
MS.gene064492.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.46e-16	82.8
MS.gene064492.t1	AT1G67530	41.463	82	47	1	24	105	262	342	1.46e-16	82.8
MS.gene064492.t1	AT3G54790	43.243	74	42	0	33	106	236	309	1.50e-16	82.8
MS.gene064492.t1	AT3G54790	43.243	74	42	0	33	106	236	309	1.50e-16	82.8
MS.gene064492.t1	AT3G07360	43.478	92	51	1	11	102	51	141	1.78e-16	81.6
MS.gene064492.t1	AT1G67530	41.463	82	47	1	24	105	262	342	2.43e-16	81.6
MS.gene064492.t1	AT1G24330	42.857	70	40	0	36	105	276	345	2.85e-16	82.0
MS.gene064492.t1	AT1G24330	42.857	70	40	0	36	105	276	345	2.85e-16	82.0
MS.gene064492.t1	AT2G45920	32.407	108	59	2	8	101	284	391	2.05e-15	78.2
MS.gene064492.t1	AT4G36550	40.845	71	42	0	35	105	219	289	2.29e-15	79.0
MS.gene064492.t1	AT5G57035	38.372	86	50	1	19	101	689	774	2.69e-15	79.0
MS.gene064492.t1	AT3G49060	36.585	82	52	0	23	104	723	804	1.06e-14	77.0
MS.gene064492.t1	AT1G01680	35.789	95	60	1	15	108	212	306	1.07e-14	75.1
MS.gene064492.t1	AT3G49060	36.585	82	52	0	23	104	690	771	1.08e-14	77.0
MS.gene064492.t1	AT3G49060	36.585	82	52	0	23	104	713	794	1.12e-14	77.0
MS.gene064492.t1	AT1G01660	37.879	66	41	0	36	101	499	564	1.77e-14	75.9
MS.gene064492.t1	AT1G01660	37.879	66	41	0	36	101	498	563	1.88e-14	75.9
MS.gene064492.t1	AT3G61390	42.424	66	38	0	36	101	279	344	2.08e-14	74.7
MS.gene064492.t1	AT3G61390	42.424	66	38	0	36	101	354	419	2.77e-14	75.1
MS.gene064492.t1	AT5G65500	35.227	88	57	0	14	101	702	789	2.20e-13	72.8
MS.gene064492.t1	AT5G65500	35.227	88	57	0	14	101	694	781	2.25e-13	72.8
MS.gene064492.t1	AT2G45910	37.143	70	44	0	36	105	764	833	5.28e-13	71.6
MS.gene064492.t1	AT2G45910	37.143	70	44	0	36	105	764	833	5.28e-13	71.6
MS.gene064492.t1	AT1G20780	45.588	68	32	1	39	101	27	94	1.07e-12	70.9
MS.gene064492.t1	AT1G20780	45.588	68	32	1	39	101	27	94	1.07e-12	70.9
MS.gene064492.t1	AT1G20780	45.588	68	32	1	39	101	27	94	1.07e-12	70.9
MS.gene064492.t1	AT5G51270	43.939	66	36	1	36	101	736	800	1.15e-12	70.5
MS.gene064492.t1	AT5G51270	43.939	66	36	1	36	101	736	800	1.15e-12	70.5
MS.gene064492.t1	AT2G23140	38.571	70	43	0	33	102	231	300	1.17e-12	70.5
MS.gene064492.t1	AT2G23140	38.571	70	43	0	33	102	228	297	1.18e-12	70.5
MS.gene064492.t1	AT2G23140	38.571	70	43	0	33	102	228	297	1.18e-12	70.5
MS.gene064492.t1	AT2G23140	38.571	70	43	0	33	102	39	108	1.51e-12	70.1
MS.gene064492.t1	AT2G23140	38.571	70	43	0	33	102	39	108	1.51e-12	70.1
MS.gene064492.t1	AT1G56040	41.538	65	38	0	36	100	354	418	2.01e-12	68.9
MS.gene064492.t1	AT1G01670	36.000	75	48	0	31	105	206	280	2.12e-12	67.8
MS.gene064492.t1	AT5G67340	35.000	80	52	0	29	108	234	313	3.04e-12	69.3
MS.gene064492.t1	AT5G67340	35.000	80	52	0	29	108	234	313	3.04e-12	69.3
MS.gene064492.t1	AT2G19410	34.884	86	52	2	19	101	706	790	3.76e-12	68.9
MS.gene064492.t1	AT2G19410	34.884	86	52	2	19	101	692	776	3.93e-12	68.9
MS.gene064492.t1	AT2G19410	34.884	86	52	2	19	101	727	811	4.33e-12	68.9
MS.gene064492.t1	AT1G01670	36.000	75	48	0	31	105	290	364	4.49e-12	67.8
MS.gene064492.t1	AT1G01670	36.000	75	48	0	31	105	290	364	4.49e-12	67.8
MS.gene064492.t1	AT1G56030	40.000	65	39	0	36	100	324	388	5.18e-12	67.8
MS.gene064492.t1	AT1G76390	41.379	87	43	3	23	101	10	96	7.30e-12	68.2
MS.gene064492.t1	AT1G76390	41.379	87	43	3	23	101	10	96	7.30e-12	68.2
MS.gene064492.t1	AT4G25160	40.845	71	41	1	31	101	762	831	8.50e-12	67.8
MS.gene064492.t1	AT5G61560	40.580	69	40	1	36	104	724	791	1.31e-11	67.4
MS.gene064492.t1	AT5G61560	40.580	69	40	1	36	104	726	793	1.36e-11	67.4
MS.gene064492.t1	AT5G61560	40.580	69	40	1	36	104	726	793	1.36e-11	67.4
MS.gene064492.t1	AT5G61560	40.580	69	40	1	36	104	745	812	1.43e-11	67.0
MS.gene064492.t1	AT5G61560	40.580	69	40	1	36	104	745	812	1.43e-11	67.0

Find 74 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTTCTTTGTTGATTA+AGG	0.152854	5.3:+62895530	MS.gene064492:CDS
TGTCTTTGTGATAGCCAAAA+TGG	0.207784	5.3:+62895807	MS.gene064492:CDS
TGAAGGGATTGTTGAATCTT+TGG	0.251918	5.3:+62895581	MS.gene064492:CDS
TTGCTATTTGCACCCCATTT+TGG	0.252053	5.3:-62895821	None:intergenic
ATTCTTGTTGATGCTGAAAA+AGG	0.259858	5.3:+62895753	MS.gene064492:CDS
TTCATCAAGTCTAGAGTTAC+TGG	0.261782	5.3:-62894940	None:intergenic
TGAGAAGAACGTTGTTCTTT+TGG	0.273407	5.3:+62895374	MS.gene064492:CDS
TGAATTGGTGAGAAAGATAA+AGG	0.287993	5.3:+62895251	MS.gene064492:CDS
ATCAAATTCTTTGAGCTGTT+TGG	0.315007	5.3:+62895467	MS.gene064492:CDS
ATTGGAGAGGTTTGTTGAAT+TGG	0.321773	5.3:+62895707	MS.gene064492:CDS
AAAGAGTAGGAAAGTGATAT+TGG	0.342934	5.3:+62895689	MS.gene064492:CDS
GTAACTTCATAACGAGTTAC+TGG	0.352002	5.3:-62895171	None:intergenic
TTGGAGAGGTTTGTTGAATT+GGG	0.357448	5.3:+62895708	MS.gene064492:CDS
ATTCTTTGAGCTGTTTGGTT+TGG	0.357701	5.3:+62895472	MS.gene064492:CDS
GGAGTTTGTGAGAAAGCTTT+AGG	0.360911	5.3:+62895774	MS.gene064492:CDS
AAGTTACTGATACGTGTAAT+AGG	0.361648	5.3:+62895187	MS.gene064492:CDS
GTGTGTTGATTCATCATCAT+TGG	0.376962	5.3:+62896094	MS.gene064492:CDS
TGATTCTTTCTCACGTGGTT+TGG	0.380428	5.3:+62895350	MS.gene064492:CDS
GTTAAGGTTAATGTTGAGAT+TGG	0.382429	5.3:+62895612	MS.gene064492:CDS
TATCATGGACAAAAGGTAGT+AGG	0.385260	5.3:+62894824	MS.gene064492:CDS
ATCTTTGGTCAACATGGTTA+AGG	0.388977	5.3:+62895596	MS.gene064492:CDS
TAAATTAGAATGGCTTCTTA+AGG	0.391586	5.3:-62896127	None:intergenic
GTCTCTATCGTAGGTTATGC+CGG	0.413834	5.3:-62894983	None:intergenic
TGCTTCTCTTGTTCTTGCTA+AGG	0.419188	5.3:+62895323	MS.gene064492:CDS
GGTGAGAAAGATAAAGGGTT+GGG	0.424541	5.3:+62895257	MS.gene064492:CDS
ATGCCGGTTGAAAGTGTAAC+GGG	0.424846	5.3:-62894967	None:intergenic
GAGGTTTGTTGAATTGGGTT+TGG	0.429998	5.3:+62895713	MS.gene064492:CDS
ATTCTACGAAGGGAGTGATT+TGG	0.431522	5.3:-62895084	None:intergenic
TTCGTAGAATGATTCAAGAT+TGG	0.433178	5.3:+62895097	MS.gene064492:CDS
GGAAATGAATGTCGAGTCAT+TGG	0.449279	5.3:+62895551	MS.gene064492:CDS
GTCTTTGTGATAGCCAAAAT+GGG	0.454330	5.3:+62895808	MS.gene064492:CDS
TTGATTGAGGCAATTCAACT+TGG	0.459886	5.3:+62895963	MS.gene064492:CDS
AAGAAGGTTATAGTTTCAAA+TGG	0.465955	5.3:+62895300	MS.gene064492:CDS
GACCCCGTTACACTTTCAAC+CGG	0.468981	5.3:+62894964	MS.gene064492:CDS
GAATTGGTGAGAAAGATAAA+GGG	0.484864	5.3:+62895252	MS.gene064492:CDS
TTACTGGAATACGTGGAGTT+GGG	0.486770	5.3:-62895155	None:intergenic
GAGTCATTGGTGAAAATTGA+AGG	0.487284	5.3:+62895564	MS.gene064492:CDS
CAATTCAGTAGCTTTCTCCT+TGG	0.500670	5.3:-62896036	None:intergenic
ATCATGGACAAAAGGTAGTA+GGG	0.502085	5.3:+62894825	MS.gene064492:CDS
TGTGTTCAACACCGTTCCTA+TGG	0.506903	5.3:+62895120	MS.gene064492:CDS
TTCTTTCGACGCCATAGGAA+CGG	0.509833	5.3:-62895131	None:intergenic
TCCTCATAGATAAATTAGAA+TGG	0.519150	5.3:-62896137	None:intergenic
CAAGATTTGTGACAAGGCTG+AGG	0.519433	5.3:+62895932	MS.gene064492:CDS
TACGTGTAATAGGATTCTTT+CGG	0.519461	5.3:+62895197	MS.gene064492:CDS
GTTGGGGAAAAGAGAGTGAG+AGG	0.520190	5.3:+62895274	MS.gene064492:CDS
TTCGATGCTGTCTCTATCGT+AGG	0.522127	5.3:-62894992	None:intergenic
GTTACTGGAATACGTGGAGT+TGG	0.523278	5.3:-62895156	None:intergenic
TGGTTTACTCATCAAAGAGT+AGG	0.526893	5.3:+62895676	MS.gene064492:CDS
GAATTGTTGAAATTGTTGAA+TGG	0.533871	5.3:+62896053	MS.gene064492:CDS
TGTTGAATCTTTGGTCAACA+TGG	0.533879	5.3:+62895590	MS.gene064492:CDS
AAAAGCTTCTTAATAACAAG+AGG	0.544112	5.3:-62895864	None:intergenic
AGAGAGTGAGAGGAACAAGA+AGG	0.555137	5.3:+62895284	MS.gene064492:CDS
ATCTTGAATCATTCTACGAA+GGG	0.557256	5.3:-62895094	None:intergenic
TGATTGAGGCAATTCAACTT+GGG	0.559461	5.3:+62895964	MS.gene064492:CDS
TGGGATTCTTTCGACGCCAT+AGG	0.559846	5.3:-62895136	None:intergenic
TTCATGCAATGATTTACAAG+TGG	0.567554	5.3:+62894888	MS.gene064492:CDS
GTGAGAAAGATAAAGGGTTG+GGG	0.581859	5.3:+62895258	MS.gene064492:CDS
ACTGGGCAACGAAAATGAGT+TGG	0.587838	5.3:-62894922	None:intergenic
AGTCATTGGTGAAAATTGAA+GGG	0.590339	5.3:+62895565	MS.gene064492:CDS
GTAGGAAAGTGATATTGGAG+AGG	0.596224	5.3:+62895694	MS.gene064492:CDS
ATTCTTTCGGCAGCGCAACA+AGG	0.598395	5.3:+62895210	MS.gene064492:CDS
GTGATTTGGTACGATGTCAA+AGG	0.600729	5.3:-62895070	None:intergenic
TCATCAAGTCTAGAGTTACT+GGG	0.601581	5.3:-62894939	None:intergenic
TGTTTACAAGATTTGTGACA+AGG	0.613076	5.3:+62895926	MS.gene064492:CDS
AATCTTGAATCATTCTACGA+AGG	0.617281	5.3:-62895095	None:intergenic
TGGTGAGAAAGATAAAGGGT+TGG	0.619284	5.3:+62895256	MS.gene064492:CDS
GATGCGTCCTTTGTCTGAAG+AGG	0.622117	5.3:+62895422	MS.gene064492:CDS
ATGCGTCCTTTGTCTGAAGA+GGG	0.633362	5.3:+62895423	MS.gene064492:CDS
AATCTACCCTCTTCAGACAA+AGG	0.640481	5.3:-62895429	None:intergenic
TATGCCGGTTGAAAGTGTAA+CGG	0.642906	5.3:-62894968	None:intergenic
TAACGAGTTACTGGAATACG+TGG	0.686345	5.3:-62895162	None:intergenic
TCTTTGTGATAGCCAAAATG+GGG	0.736673	5.3:+62895809	MS.gene064492:CDS
AGGTTGTGCTGAGAGTACCA+AGG	0.750134	5.3:+62896019	MS.gene064492:CDS
TGCCGGTTGAAAGTGTAACG+GGG	0.753225	5.3:-62894966	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCAACAAAAAATTTCAACT+AGG	+	chr5.3:62895505-62895524	MS.gene064492:CDS	20.0%
!!!	TTTGTCAACAATTTTTCATT+TGG	+	chr5.3:62895656-62895675	MS.gene064492:CDS	20.0%
!	AAAAGCTTCTTAATAACAAG+AGG	-	chr5.3:62895867-62895886	None:intergenic	25.0%
!	AAGAAGGTTATAGTTTCAAA+TGG	+	chr5.3:62895300-62895319	MS.gene064492:CDS	25.0%
!	GAATTGTTGAAATTGTTGAA+TGG	+	chr5.3:62896053-62896072	MS.gene064492:CDS	25.0%
!!	AAATGCTTCTTTGTTGATTA+AGG	+	chr5.3:62895530-62895549	MS.gene064492:CDS	25.0%
!!	TGTTATTAAGAAGCTTTTGA+GGG	+	chr5.3:62895869-62895888	MS.gene064492:CDS	25.0%
!!!	TGTTAACTCTGTTTTTGTAA+CGG	-	chr5.3:62895049-62895068	None:intergenic	25.0%
!!!	TTGTTATTAAGAAGCTTTTG+AGG	+	chr5.3:62895868-62895887	MS.gene064492:CDS	25.0%
	AAAGAGTAGGAAAGTGATAT+TGG	+	chr5.3:62895689-62895708	MS.gene064492:CDS	30.0%
	AAGTTACTGATACGTGTAAT+AGG	+	chr5.3:62895187-62895206	MS.gene064492:CDS	30.0%
	AATCTTGAATCATTCTACGA+AGG	-	chr5.3:62895098-62895117	None:intergenic	30.0%
	AGTCATTGGTGAAAATTGAA+GGG	+	chr5.3:62895565-62895584	MS.gene064492:CDS	30.0%
	ATCAAATTCTTTGAGCTGTT+TGG	+	chr5.3:62895467-62895486	MS.gene064492:CDS	30.0%
	ATCGAAAAATGGTTTGAATC+AGG	+	chr5.3:62895009-62895028	MS.gene064492:CDS	30.0%
	ATCTTGAATCATTCTACGAA+GGG	-	chr5.3:62895097-62895116	None:intergenic	30.0%
	ATTCTTGTTGATGCTGAAAA+AGG	+	chr5.3:62895753-62895772	MS.gene064492:CDS	30.0%
	GAATTGGTGAGAAAGATAAA+GGG	+	chr5.3:62895252-62895271	MS.gene064492:CDS	30.0%
	GTTAAGGTTAATGTTGAGAT+TGG	+	chr5.3:62895612-62895631	MS.gene064492:CDS	30.0%
	TACGTGTAATAGGATTCTTT+CGG	+	chr5.3:62895197-62895216	MS.gene064492:CDS	30.0%
	TGAATTGGTGAGAAAGATAA+AGG	+	chr5.3:62895251-62895270	MS.gene064492:CDS	30.0%
	TGTTTACAAGATTTGTGACA+AGG	+	chr5.3:62895926-62895945	MS.gene064492:CDS	30.0%
	TTCATGCAATGATTTACAAG+TGG	+	chr5.3:62894888-62894907	MS.gene064492:CDS	30.0%
	TTCGTAGAATGATTCAAGAT+TGG	+	chr5.3:62895097-62895116	MS.gene064492:CDS	30.0%
!!	GGGTTTGGTTTCAATTTTAT+TGG	+	chr5.3:62895728-62895747	MS.gene064492:CDS	30.0%
!!	TGTTCTTTTGGAAGAGATTT+TGG	+	chr5.3:62895386-62895405	MS.gene064492:CDS	30.0%
!!!	ATGGTTTTATCATGGACAAA+AGG	+	chr5.3:62894817-62894836	MS.gene064492:CDS	30.0%
!!!	GTTAACTCTGTTTTTGTAAC+GGG	-	chr5.3:62895048-62895067	None:intergenic	30.0%
!!!	TGAGGAAAGTATTTTGATTG+AGG	+	chr5.3:62895950-62895969	MS.gene064492:CDS	30.0%
	ATCATGGACAAAAGGTAGTA+GGG	+	chr5.3:62894825-62894844	MS.gene064492:CDS	35.0%
	ATCTTTGGTCAACATGGTTA+AGG	+	chr5.3:62895596-62895615	MS.gene064492:CDS	35.0%
	ATTCTTTGAGCTGTTTGGTT+TGG	+	chr5.3:62895472-62895491	MS.gene064492:CDS	35.0%
	GAGTCATTGGTGAAAATTGA+AGG	+	chr5.3:62895564-62895583	MS.gene064492:CDS	35.0%
	GTAACTTCATAACGAGTTAC+TGG	-	chr5.3:62895174-62895193	None:intergenic	35.0%
	GTCTTTGTGATAGCCAAAAT+GGG	+	chr5.3:62895808-62895827	MS.gene064492:CDS	35.0%
	TATCATGGACAAAAGGTAGT+AGG	+	chr5.3:62894824-62894843	MS.gene064492:CDS	35.0%
	TCTTTGTGATAGCCAAAATG+GGG	+	chr5.3:62895809-62895828	MS.gene064492:CDS	35.0%
	TGAAGGGATTGTTGAATCTT+TGG	+	chr5.3:62895581-62895600	MS.gene064492:CDS	35.0%
	TGATTGAGGCAATTCAACTT+GGG	+	chr5.3:62895964-62895983	MS.gene064492:CDS	35.0%
	TGGTTTACTCATCAAAGAGT+AGG	+	chr5.3:62895676-62895695	MS.gene064492:CDS	35.0%
	TGTCTTTGTGATAGCCAAAA+TGG	+	chr5.3:62895807-62895826	MS.gene064492:CDS	35.0%
	TGTTGAATCTTTGGTCAACA+TGG	+	chr5.3:62895590-62895609	MS.gene064492:CDS	35.0%
	TTGATTGAGGCAATTCAACT+TGG	+	chr5.3:62895963-62895982	MS.gene064492:CDS	35.0%
!	AGAGGGTAGATTTGTCTTTT+TGG	+	chr5.3:62895440-62895459	MS.gene064492:CDS	35.0%
!	ATAGAGACAGCATCGAAAAA+TGG	+	chr5.3:62894998-62895017	MS.gene064492:CDS	35.0%
!	GAGGGTAGATTTGTCTTTTT+GGG	+	chr5.3:62895441-62895460	MS.gene064492:CDS	35.0%
!	TCATCAAGTCTAGAGTTACT+GGG	-	chr5.3:62894942-62894961	None:intergenic	35.0%
!	TGAGAAGAACGTTGTTCTTT+TGG	+	chr5.3:62895374-62895393	MS.gene064492:CDS	35.0%
!	TTCATCAAGTCTAGAGTTAC+TGG	-	chr5.3:62894943-62894962	None:intergenic	35.0%
!!	ATTGGAGAGGTTTGTTGAAT+TGG	+	chr5.3:62895707-62895726	MS.gene064492:CDS	35.0%
!!	GTGTGTTGATTCATCATCAT+TGG	+	chr5.3:62896094-62896113	MS.gene064492:CDS	35.0%
!!	GTTTTTGATTCTTTCTCACG+TGG	+	chr5.3:62895345-62895364	MS.gene064492:CDS	35.0%
!!	TTGGAGAGGTTTGTTGAATT+GGG	+	chr5.3:62895708-62895727	MS.gene064492:CDS	35.0%
!!!	CTACTTGTTTTGTTGCAAGT+AGG	+	chr5.3:62895999-62896018	MS.gene064492:CDS	35.0%
	AATCTACCCTCTTCAGACAA+AGG	-	chr5.3:62895432-62895451	None:intergenic	40.0%
	ATTCTACGAAGGGAGTGATT+TGG	-	chr5.3:62895087-62895106	None:intergenic	40.0%
	CAATTCAGTAGCTTTCTCCT+TGG	-	chr5.3:62896039-62896058	None:intergenic	40.0%
	GGAAATGAATGTCGAGTCAT+TGG	+	chr5.3:62895551-62895570	MS.gene064492:CDS	40.0%
	GGAGTTTGTGAGAAAGCTTT+AGG	+	chr5.3:62895774-62895793	MS.gene064492:CDS	40.0%
	GGTGAGAAAGATAAAGGGTT+GGG	+	chr5.3:62895257-62895276	MS.gene064492:CDS	40.0%
	GTAGGAAAGTGATATTGGAG+AGG	+	chr5.3:62895694-62895713	MS.gene064492:CDS	40.0%
	GTGAGAAAGATAAAGGGTTG+GGG	+	chr5.3:62895258-62895277	MS.gene064492:CDS	40.0%
	TAACGAGTTACTGGAATACG+TGG	-	chr5.3:62895165-62895184	None:intergenic	40.0%
	TGATTCTTTCTCACGTGGTT+TGG	+	chr5.3:62895350-62895369	MS.gene064492:CDS	40.0%
	TGCTTCTCTTGTTCTTGCTA+AGG	+	chr5.3:62895323-62895342	MS.gene064492:CDS	40.0%
	TGGTGAGAAAGATAAAGGGT+TGG	+	chr5.3:62895256-62895275	MS.gene064492:CDS	40.0%
	TTACTGGAATACGTGGAGTT+GGG	-	chr5.3:62895158-62895177	None:intergenic	40.0%
!	CTTTTCCTTTTCTTGCACGA+CGG	-	chr5.3:62894855-62894874	None:intergenic	40.0%
!	TATGCCGGTTGAAAGTGTAA+CGG	-	chr5.3:62894971-62894990	None:intergenic	40.0%
!!	CGAGAGCAAGTGTTTTGAAT+TGG	+	chr5.3:62895236-62895255	MS.gene064492:CDS	40.0%
!!	GAGGTTTGTTGAATTGGGTT+TGG	+	chr5.3:62895713-62895732	MS.gene064492:CDS	40.0%
!!	GTGATTTGGTACGATGTCAA+AGG	-	chr5.3:62895073-62895092	None:intergenic	40.0%
!!!	AGATTTTGGAAGTGCTGACA+TGG	+	chr5.3:62895400-62895419	MS.gene064492:CDS	40.0%
!!!	TTGCTATTTGCACCCCATTT+TGG	-	chr5.3:62895824-62895843	None:intergenic	40.0%
	ACTGGGCAACGAAAATGAGT+TGG	-	chr5.3:62894925-62894944	None:intergenic	45.0%
	AGAGAGTGAGAGGAACAAGA+AGG	+	chr5.3:62895284-62895303	MS.gene064492:CDS	45.0%
	ATGCGTCCTTTGTCTGAAGA+GGG	+	chr5.3:62895423-62895442	MS.gene064492:CDS	45.0%
	CAAGATTTGTGACAAGGCTG+AGG	+	chr5.3:62895932-62895951	MS.gene064492:CDS	45.0%
	GTCTCTATCGTAGGTTATGC+CGG	-	chr5.3:62894986-62895005	None:intergenic	45.0%
	GTTACTGGAATACGTGGAGT+TGG	-	chr5.3:62895159-62895178	None:intergenic	45.0%
	TGTGTTCAACACCGTTCCTA+TGG	+	chr5.3:62895120-62895139	MS.gene064492:CDS	45.0%
	TTCGATGCTGTCTCTATCGT+AGG	-	chr5.3:62894995-62895014	None:intergenic	45.0%
	TTCTTTCGACGCCATAGGAA+CGG	-	chr5.3:62895134-62895153	None:intergenic	45.0%
!	ATGCCGGTTGAAAGTGTAAC+GGG	-	chr5.3:62894970-62894989	None:intergenic	45.0%
!	GTTTTCCGTCGTGCAAGAAA+AGG	+	chr5.3:62894847-62894866	MS.gene064492:CDS	45.0%
	ATTCTTTCGGCAGCGCAACA+AGG	+	chr5.3:62895210-62895229	MS.gene064492:CDS	50.0%
	GACCCCGTTACACTTTCAAC+CGG	+	chr5.3:62894964-62894983	MS.gene064492:CDS	50.0%
	GATGCGTCCTTTGTCTGAAG+AGG	+	chr5.3:62895422-62895441	MS.gene064492:CDS	50.0%
	GTTGGGGAAAAGAGAGTGAG+AGG	+	chr5.3:62895274-62895293	MS.gene064492:CDS	50.0%
	TGGGATTCTTTCGACGCCAT+AGG	-	chr5.3:62895139-62895158	None:intergenic	50.0%
!	TGCCGGTTGAAAGTGTAACG+GGG	-	chr5.3:62894969-62894988	None:intergenic	50.0%
!!	AGGTTGTGCTGAGAGTACCA+AGG	+	chr5.3:62896019-62896038	MS.gene064492:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	62894817	62896145	62894817	ID=MS.gene064492
chr5.3	mRNA	62894817	62896145	62894817	ID=MS.gene064492.t1;Parent=MS.gene064492
chr5.3	exon	62894817	62896145	62894817	ID=MS.gene064492.t1.exon1;Parent=MS.gene064492.t1
chr5.3	CDS	62894817	62896145	62894817	ID=cds.MS.gene064492.t1;Parent=MS.gene064492.t1

Gene Sequence

>MS.gene064492

ATGGTTTTATCATGGACAAAAGGTAGTAGGGTTTTCCGTCGTGCAAGAAAAGGAAAAGAGTTAATATCAAGTTCATGCAATGATTTACAAGTGGAGATAGCAATTCCAACTCATTTTCGTTGCCCAGTAACTCTAGACTTGATGAAAGACCCCGTTACACTTTCAACCGGCATAACCTACGATAGAGACAGCATCGAAAAATGGTTTGAATCAGGTAACGACACATGTCCCGTTACAAAAACAGAGTTAACATCCTTTGACATCGTACCAAATCACTCCCTTCGTAGAATGATTCAAGATTGGTGTGTTCAACACCGTTCCTATGGCGTCGAAAGAATCCCAACTCCACGTATTCCAGTAACTCGTTATGAAGTTACTGATACGTGTAATAGGATTCTTTCGGCAGCGCAACAAGGAGACGAGAGCAAGTGTTTTGAATTGGTGAGAAAGATAAAGGGTTGGGGAAAAGAGAGTGAGAGGAACAAGAAGGTTATAGTTTCAAATGGTGCTTCTCTTGTTCTTGCTAAGGTTTTTGATTCTTTCTCACGTGGTTTGGTTGAGAAGAACGTTGTTCTTTTGGAAGAGATTTTGGAAGTGCTGACATGGATGCGTCCTTTGTCTGAAGAGGGTAGATTTGTCTTTTTGGGATCATCAAATTCTTTGAGCTGTTTGGTTTGGTTCTTAAATGATCAACAAAAAATTTCAACTAGGCAAAATGCTTCTTTGTTGATTAAGGAAATGAATGTCGAGTCATTGGTGAAAATTGAAGGGATTGTTGAATCTTTGGTCAACATGGTTAAGGTTAATGTTGAGATTGGAAGTGCTTCTACAAAAGCATGTTTGTCAACAATTTTTCATTTGGTTTACTCATCAAAGAGTAGGAAAGTGATATTGGAGAGGTTTGTTGAATTGGGTTTGGTTTCAATTTTATTGGAGATTCTTGTTGATGCTGAAAAAGGAGTTTGTGAGAAAGCTTTAGGTGTGTTGAATTGTCTTTGTGATAGCCAAAATGGGGTGCAAATAGCAAAATCAAATGCTTTGACTTTGCCTCTTGTTATTAAGAAGCTTTTGAGGGTTTCTGAATTGAGTTCTAGTTTTGTTGTTTCTATTGTTTACAAGATTTGTGACAAGGCTGAGGAAAGTATTTTGATTGAGGCAATTCAACTTGGGATGTTTCAAAAACTACTTGTTTTGTTGCAAGTAGGTTGTGCTGAGAGTACCAAGGAGAAAGCTACTGAATTGTTGAAATTGTTGAATGGTTATAAAAGCAAAGCAGAGTGTGTTGATTCATCATCATTGGATTTCACACACCTTAAGAAGCCATTCTAA

Protein sequence

>MS.gene064492.t1

MVLSWTKGSRVFRRARKGKELISSSCNDLQVEIAIPTHFRCPVTLDLMKDPVTLSTGITYDRDSIEKWFESGNDTCPVTKTELTSFDIVPNHSLRRMIQDWCVQHRSYGVERIPTPRIPVTRYEVTDTCNRILSAAQQGDESKCFELVRKIKGWGKESERNKKVIVSNGASLVLAKVFDSFSRGLVEKNVVLLEEILEVLTWMRPLSEEGRFVFLGSSNSLSCLVWFLNDQQKISTRQNASLLIKEMNVESLVKIEGIVESLVNMVKVNVEIGSASTKACLSTIFHLVYSSKSRKVILERFVELGLVSILLEILVDAEKGVCEKALGVLNCLCDSQNGVQIAKSNALTLPLVIKKLLRVSELSSSFVVSIVYKICDKAEESILIEAIQLGMFQKLLVLLQVGCAESTKEKATELLKLLNGYKSKAECVDSSSLDFTHLKKPF