AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033735.t1 MTR_7g084350 92.899 338 24 0 1 338 28 365 0.0 656
MS.gene033735.t1 MTR_3g467560 71.217 337 93 2 1 336 54 387 3.66e-178 499
MS.gene033735.t1 MTR_7g084620 70.968 341 91 5 1 338 30 365 4.05e-176 493
MS.gene033735.t1 MTR_7g084330 67.155 341 89 6 1 338 13 333 2.54e-158 446
MS.gene033735.t1 MTR_3g117060 51.320 341 158 5 1 336 30 367 7.29e-116 340
MS.gene033735.t1 MTR_6g056070 40.625 320 170 4 24 338 53 357 3.46e-80 248
MS.gene033735.t1 MTR_8g076900 38.938 339 192 5 3 336 32 360 2.05e-79 246
MS.gene033735.t1 MTR_5g020940 38.953 344 194 6 3 336 42 379 9.15e-79 246
MS.gene033735.t1 MTR_4g018820 37.758 339 193 5 3 336 32 357 8.97e-78 242
MS.gene033735.t1 MTR_1g022465 42.331 326 167 6 26 336 57 376 6.42e-77 241
MS.gene033735.t1 MTR_8g077040 36.812 345 203 5 3 338 32 370 2.28e-76 239
MS.gene033735.t1 MTR_1g022455 41.358 324 174 5 26 336 57 377 1.93e-75 237
MS.gene033735.t1 MTR_3g467270 35.345 348 204 5 3 338 31 369 6.84e-75 235
MS.gene033735.t1 MTR_8g020700 39.688 320 171 4 3 316 32 335 2.19e-72 228
MS.gene033735.t1 MTR_5g046110 37.427 342 195 5 2 336 91 420 1.64e-67 218
MS.gene033735.t1 MTR_8g020690 35.294 340 201 4 3 336 32 358 5.57e-66 212
MS.gene033735.t1 MTR_7g084340 80.833 120 23 0 218 337 11 130 1.85e-64 200
MS.gene033735.t1 MTR_8g076900 42.727 220 115 3 3 217 32 245 1.20e-53 177
MS.gene033735.t1 MTR_3g052700 36.000 300 175 6 26 314 71 364 4.28e-53 179
MS.gene033735.t1 MTR_8g020700 42.534 221 115 3 3 217 32 246 4.53e-52 173
MS.gene033735.t1 MTR_5g093900 34.056 323 194 7 26 335 73 389 1.14e-51 176
MS.gene033735.t1 MTR_3g054330 36.093 302 173 7 26 314 63 357 1.94e-50 172
MS.gene033735.t1 MTR_1g108660 31.646 316 178 7 26 331 42 329 8.56e-50 169
MS.gene033735.t1 MTR_8g077030 29.582 311 129 9 6 314 34 256 8.55e-29 116
MS.gene033735.t1 MTR_6g015620 44.094 127 66 2 213 336 12 136 1.38e-26 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033735.t1 AT1G19640 38.483 356 197 5 4 337 33 388 1.62e-73 232
MS.gene033735.t1 AT5G66430 40.615 325 173 7 2 318 23 335 1.27e-72 229
MS.gene033735.t1 AT3G11480 37.941 340 190 4 3 336 54 378 8.88e-70 223
MS.gene033735.t1 AT5G38020 39.275 331 175 7 3 318 29 348 1.07e-68 219
MS.gene033735.t1 AT5G38020 39.688 320 167 7 14 318 1 309 1.28e-67 215
MS.gene033735.t1 AT5G04370 39.296 341 187 8 2 336 63 389 3.96e-65 211
MS.gene033735.t1 AT5G04370 39.296 341 187 8 2 336 82 408 4.59e-65 211
MS.gene033735.t1 AT5G04370 39.296 341 187 8 2 336 68 394 5.83e-65 211
MS.gene033735.t1 AT3G21950 39.490 314 164 6 14 312 40 342 2.21e-64 208
MS.gene033735.t1 AT4G36470 36.957 322 176 8 9 316 41 349 1.68e-62 203
MS.gene033735.t1 AT5G04380 36.111 324 189 4 6 323 64 375 1.49e-61 201
MS.gene033735.t1 AT5G04380 36.392 316 183 4 14 323 1 304 2.04e-61 199
MS.gene033735.t1 AT3G21950 35.838 346 164 7 14 312 40 374 1.15e-57 192
MS.gene033735.t1 AT5G04380 35.185 324 185 5 6 323 60 364 2.19e-56 188
MS.gene033735.t1 AT2G14060 34.146 328 200 6 2 322 36 354 3.91e-55 184
MS.gene033735.t1 AT1G15125 32.258 341 193 11 10 337 33 348 3.30e-49 168
MS.gene033735.t1 AT5G55250 32.602 319 195 7 6 311 9 320 4.03e-48 165
MS.gene033735.t1 AT5G55250 32.602 319 195 7 6 311 47 358 1.32e-47 165
MS.gene033735.t1 AT1G68040 30.655 336 194 11 10 333 49 357 7.06e-43 152
MS.gene033735.t1 AT5G37970 31.046 306 177 6 25 319 60 342 1.41e-42 151
MS.gene033735.t1 AT5G37990 30.392 306 179 6 25 319 60 342 1.74e-42 151
MS.gene033735.t1 AT5G38100 31.307 329 186 10 2 317 13 314 2.93e-41 147
MS.gene033735.t1 AT5G38780 30.659 349 198 10 2 332 36 358 4.34e-41 147
MS.gene033735.t1 AT5G38780 31.307 329 183 9 22 332 6 309 6.27e-41 145
MS.gene033735.t1 AT5G38100 31.307 329 186 10 2 317 36 337 7.71e-41 146
MS.gene033735.t1 AT2G14060 39.487 195 110 2 2 196 36 222 6.63e-40 141
MS.gene033735.t1 AT4G26420 28.528 326 208 7 21 331 54 369 2.87e-35 132
MS.gene033735.t1 AT5G56300 28.267 329 202 10 25 333 68 382 7.70e-35 131
MS.gene033735.t1 AT1G66720 27.987 318 189 7 2 309 35 322 1.80e-33 127
MS.gene033735.t1 AT3G44870 29.592 294 166 8 26 309 53 315 4.29e-33 126
MS.gene033735.t1 AT1G66690 29.801 302 161 8 26 309 55 323 1.81e-31 121
MS.gene033735.t1 AT1G15125 33.937 221 122 8 10 220 33 239 9.90e-31 117
MS.gene033735.t1 AT1G66700 27.797 295 176 6 26 309 55 323 1.30e-30 119
MS.gene033735.t1 AT3G44860 32.365 241 137 6 26 256 53 277 1.84e-30 119
MS.gene033735.t1 AT3G44840 28.179 291 177 7 26 309 53 318 4.16e-30 117
MS.gene033735.t1 AT5G38100 30.396 227 140 7 2 224 36 248 1.94e-22 94.7
MS.gene033735.t1 AT5G38100 30.396 227 140 7 2 224 36 248 2.25e-22 94.7
MS.gene033735.t1 AT4G26420 30.857 175 109 4 25 195 58 224 7.96e-22 93.6
MS.gene033735.t1 AT4G26420 30.857 175 109 4 25 195 46 212 8.78e-22 93.6
MS.gene033735.t1 AT1G66700 32.941 170 95 4 26 190 55 210 1.98e-20 89.7

Find 55 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTCTTGGTTGAATATTT+TGG 0.117266 7.4:+22639708 None:intergenic
GATGAGTGGTTCTGCTTTCT+TGG 0.159579 7.4:+22639695 None:intergenic
ATAGATCATTGAGGTAAATT+TGG 0.201496 7.4:+22640701 None:intergenic
TTTAGTTGAAGAAGCAAAAT+TGG 0.215168 7.4:-22638448 MS.gene033735:CDS
CTCTTTCTTGTTGAATGCTT+TGG 0.242144 7.4:+22640632 None:intergenic
GTGATGGTACTAACTTTAAT+TGG 0.251253 7.4:-22639549 MS.gene033735:CDS
TTCAGGACCAAATGCACTTA+TGG 0.271528 7.4:-22640791 MS.gene033735:CDS
TGTTGGAAAGTAGTTGATTT+AGG 0.276313 7.4:-22640820 MS.gene033735:CDS
GTTGATTTAGGTTGTTCTTC+AGG 0.296247 7.4:-22640808 MS.gene033735:CDS
GATCAACAATATCTTCTTGC+AGG 0.314712 7.4:+22638307 None:intergenic
GCAACACCAGGGAGCTTCTA+TGG 0.337472 7.4:-22640580 MS.gene033735:CDS
TCTATATCATCCTACTATAA+AGG 0.359099 7.4:-22638409 MS.gene033735:CDS
GAGACAACCATAAGTGCATT+TGG 0.372630 7.4:+22640784 None:intergenic
GAGGTTTATGTTGAGCAATT+TGG 0.385082 7.4:-22639627 MS.gene033735:CDS
TGTTTCACTTCCTTTATAGT+AGG 0.392703 7.4:+22638399 None:intergenic
GAATTTGAAAATGATGATAT+CGG 0.417336 7.4:-22640860 MS.gene033735:CDS
TTTCTTGTTGAATGCTTTGG+TGG 0.417731 7.4:+22640635 None:intergenic
ATTCTTCCTATTGTGTCCAT+TGG 0.424057 7.4:-22640522 MS.gene033735:CDS
TGATGGTACTAACTTTAATT+GGG 0.439302 7.4:-22639548 MS.gene033735:CDS
CGACCTTCTTTAAGGACACT+TGG 0.439820 7.4:+22638098 None:intergenic
AAACAAGTGATCGAGGTAGA+AGG 0.446801 7.4:-22638381 MS.gene033735:CDS
TGATAGCCAATGGACACAAT+AGG 0.448336 7.4:+22640516 None:intergenic
TTAATTGGGAGAGAAAATAA+TGG 0.449128 7.4:-22639534 MS.gene033735:CDS
TTGAGGTAAATTTGGAATGC+TGG 0.456080 7.4:+22640709 None:intergenic
TGAATGCTTTGGTGGAAATC+AGG 0.457567 7.4:+22640643 None:intergenic
GCATGTATGAAGCATGTTCC+AGG 0.460803 7.4:+22640601 None:intergenic
AAAACAAGCTCTGTGATACT+TGG 0.480684 7.4:+22638248 None:intergenic
GAAAGCAGAACCACTCATCA+AGG 0.487187 7.4:-22639691 MS.gene033735:CDS
GAGCCCTCCATCGGTGTATG+AGG 0.490938 7.4:-22639646 MS.gene033735:CDS
TTTATAGTAGGATGATATAG+AGG 0.491881 7.4:+22638411 None:intergenic
CAAGAAAGAGGAGAGAATCC+TGG 0.507141 7.4:-22640619 MS.gene033735:CDS
ATTAGCTTAAGCTTAAACCA+TGG 0.513101 7.4:-22640736 MS.gene033735:CDS
TCATGGATGAATTATTCTCA+AGG 0.523360 7.4:-22638191 MS.gene033735:CDS
TGCTTCATACATGCAACACC+AGG 0.523693 7.4:-22640592 MS.gene033735:CDS
CAAAGCATTCAACAAGAAAG+AGG 0.526195 7.4:-22640631 MS.gene033735:CDS
TTAGCTTAAGCTTAAACCAT+GGG 0.541808 7.4:-22640735 MS.gene033735:CDS
TTTGGAATGCTGGTAGCCCA+TGG 0.567597 7.4:+22640719 None:intergenic
ACGATCGGAAGAACTAGCAT+TGG 0.573764 7.4:-22639577 MS.gene033735:CDS
TAAACCTCATACACCGATGG+AGG 0.585410 7.4:+22639642 None:intergenic
TGAGTTTGGTGAAAATATCA+TGG 0.591260 7.4:-22638208 MS.gene033735:CDS
AGTCTTCCATAGAAGCTCCC+TGG 0.600448 7.4:+22640574 None:intergenic
ATCAGGTAGTAACTTCAAGA+TGG 0.603639 7.4:+22640660 None:intergenic
AACTTACTTGTGATAGCCAA+TGG 0.609905 7.4:+22640506 None:intergenic
GTCGTTGATAGCAAGATGAG+AGG 0.620263 7.4:-22638282 MS.gene033735:CDS
ACTAGCATTGGATGGTGTGA+TGG 0.625972 7.4:-22639565 MS.gene033735:CDS
AACAAGAATGAGCCCTCCAT+CGG 0.627728 7.4:-22639655 MS.gene033735:CDS
TAGATATTACCCTTGATGAG+TGG 0.628626 7.4:+22639681 None:intergenic
TCGGAAGAACTAGCATTGGA+TGG 0.630987 7.4:-22639573 MS.gene033735:CDS
AAAGCAGAACCACTCATCAA+GGG 0.632811 7.4:-22639690 MS.gene033735:CDS
GGAAGTGAAACAAGTGATCG+AGG 0.635174 7.4:-22638388 MS.gene033735:CDS
AAACCTCATACACCGATGGA+GGG 0.643833 7.4:+22639643 None:intergenic
AGGCATGGTACTCAACGAGA+TGG 0.648975 7.4:-22639487 MS.gene033735:CDS
ACATAAACCTCATACACCGA+TGG 0.652143 7.4:+22639639 None:intergenic
ACTCAACGAGATGGTCCAAG+AGG 0.710119 7.4:-22639478 MS.gene033735:intron
GCTTCATACATGCAACACCA+GGG 0.712541 7.4:-22640591 MS.gene033735:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATCTATGTAATTAT+TGG + chr7.4:22638450-22638469 None:intergenic 10.0%
!! AAAAAATCTATGTAATTATT+GGG + chr7.4:22638449-22638468 None:intergenic 10.0%
!! TCAAATAGATAAATAATAAT+AGG + chr7.4:22638953-22638972 None:intergenic 10.0%
!! TTATTATTTATCTATTTGAT+AGG - chr7.4:22638954-22638973 MS.gene033735:intron 10.0%
!!! AAGTTATATAGTTTTTTTTT+AGG + chr7.4:22639903-22639922 None:intergenic 10.0%
!!! AGTTATATAGTTTTTTTTTA+GGG + chr7.4:22639902-22639921 None:intergenic 10.0%
!!! TTTTTTTTTGAAAGATTTTA+GGG + chr7.4:22639868-22639887 None:intergenic 10.0%
!!! TTTTTTTTTTGAAAGATTTT+AGG + chr7.4:22639869-22639888 None:intergenic 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr7.4:22638983-22639002 None:intergenic 100.0%
!! AATAATAATACACAAAAAGA+AGG + chr7.4:22638841-22638860 None:intergenic 15.0%
!! ATATTAACAAAGATTCAAAT+TGG + chr7.4:22640411-22640430 None:intergenic 15.0%
!! ATTGATGAATTAAAACTATT+TGG + chr7.4:22638521-22638540 None:intergenic 15.0%
!! TTCAAAAGTAAAAATATTGT+AGG + chr7.4:22639803-22639822 None:intergenic 15.0%
!! TTGGTAAAAAAAAAATATTC+AGG + chr7.4:22638615-22638634 None:intergenic 15.0%
!!! ACAAAACATAATTTTTATTC+CGG + chr7.4:22640482-22640501 None:intergenic 15.0%
!!! CTAATAAAATTCTTATTTGT+TGG + chr7.4:22638634-22638653 None:intergenic 15.0%
!!! TATTTATCTTTTTTTGATGA+TGG - chr7.4:22638857-22638876 MS.gene033735:intron 15.0%
!! AAAAATAATTTGGGAAGTTT+AGG - chr7.4:22640070-22640089 MS.gene033735:intron 20.0%
!! AAATTGAGAACACATAATTA+AGG + chr7.4:22639034-22639053 None:intergenic 20.0%
!! AACACAATATTGAAAAAATC+TGG + chr7.4:22640867-22640886 None:intergenic 20.0%
!! AGACAAATGTACTATAAAAA+GGG - chr7.4:22638811-22638830 MS.gene033735:intron 20.0%
!! CGATAATATTGTTGTATTTA+CGG - chr7.4:22638788-22638807 MS.gene033735:intron 20.0%
!! GAATTTGAAAATGATGATAT+CGG - chr7.4:22638133-22638152 MS.gene033735:CDS 20.0%
!! GTTGAAGTCAAAAATAATTT+GGG - chr7.4:22640061-22640080 MS.gene033735:intron 20.0%
!! TCTATTGATCATCATATTAT+TGG - chr7.4:22640444-22640463 MS.gene033735:intron 20.0%
!! TGAATTAAAACTATTTGGTT+TGG + chr7.4:22638516-22638535 None:intergenic 20.0%
!! TGTTGAAGTCAAAAATAATT+TGG - chr7.4:22640060-22640079 MS.gene033735:intron 20.0%
!!! AAATTTGGTGATCTTAAAAA+AGG - chr7.4:22638749-22638768 MS.gene033735:intron 20.0%
!!! ACATTGAAGACTTTTAAAAT+CGG - chr7.4:22640651-22640670 MS.gene033735:CDS 20.0%
!!! ATATCACCATATATTTTCAT+TGG + chr7.4:22640019-22640038 None:intergenic 20.0%
!!! GATCTTGTTTTATTTGTTAA+TGG - chr7.4:22639248-22639267 MS.gene033735:intron 20.0%
!!! TTAATAAACTTTTCCTTTGT+GGG - chr7.4:22640241-22640260 MS.gene033735:intron 20.0%
!!! TTTAATAAACTTTTCCTTTG+TGG - chr7.4:22640240-22640259 MS.gene033735:intron 20.0%
! AAAGATATTAATTACTCCAC+TGG - chr7.4:22639966-22639985 MS.gene033735:intron 25.0%
! AATCAATCATATTTACCTCT+TGG + chr7.4:22639533-22639552 None:intergenic 25.0%
! ATAGATCATTGAGGTAAATT+TGG + chr7.4:22638295-22638314 None:intergenic 25.0%
! ATCAATTAAGTTAAGACTTG+TGG - chr7.4:22639740-22639759 MS.gene033735:intron 25.0%
! ATGAACTTAACTTAGTTGAT+AGG + chr7.4:22638925-22638944 None:intergenic 25.0%
! ATTTGATATCCATTTCATCT+TGG - chr7.4:22640153-22640172 MS.gene033735:intron 25.0%
! GAGACAAATGTACTATAAAA+AGG - chr7.4:22638810-22638829 MS.gene033735:intron 25.0%
! TCTATATCATCCTACTATAA+AGG - chr7.4:22640584-22640603 MS.gene033735:CDS 25.0%
! TGATGGTACTAACTTTAATT+GGG - chr7.4:22639445-22639464 MS.gene033735:intron 25.0%
! TTAATTGGGAGAGAAAATAA+TGG - chr7.4:22639459-22639478 MS.gene033735:intron 25.0%
! TTGCATCCAATGAAAATATA+TGG - chr7.4:22640010-22640029 MS.gene033735:intron 25.0%
! TTTAGTTGAAGAAGCAAAAT+TGG - chr7.4:22640545-22640564 MS.gene033735:CDS 25.0%
! TTTATAGTAGGATGATATAG+AGG + chr7.4:22640585-22640604 None:intergenic 25.0%
! TTTGTTAATGGTGGTATATT+AGG - chr7.4:22639260-22639279 MS.gene033735:intron 25.0%
!! ACACAACTTTTAATAGTTGT+TGG - chr7.4:22638156-22638175 MS.gene033735:CDS 25.0%
!! CATTTTCATATAGATCATTG+AGG + chr7.4:22638304-22638323 None:intergenic 25.0%
!! TAATAAACTTTTCCTTTGTG+GGG - chr7.4:22640242-22640261 MS.gene033735:intron 25.0%
!!! CTTGTTTTATTTGTTAATGG+TGG - chr7.4:22639251-22639270 MS.gene033735:intron 25.0%
AAATATTGTAGGAAGCGATT+AGG + chr7.4:22639792-22639811 None:intergenic 30.0%
AAATGACTTTATACTACCAG+TGG + chr7.4:22639985-22640004 None:intergenic 30.0%
AAGAGGAAACTCAATCATTT+AGG - chr7.4:22639208-22639227 MS.gene033735:intron 30.0%
AAGTTGCAATTACAAGATAG+AGG + chr7.4:22640199-22640218 None:intergenic 30.0%
AATTACAAGATAGAGGAAAG+AGG + chr7.4:22640192-22640211 None:intergenic 30.0%
ATAATACATGCAAAGGTTTG+GGG + chr7.4:22638894-22638913 None:intergenic 30.0%
CAAAGATTCAAATTGGCAAA+AGG + chr7.4:22640404-22640423 None:intergenic 30.0%
CATAATACATGCAAAGGTTT+GGG + chr7.4:22638895-22638914 None:intergenic 30.0%
GTGATGGTACTAACTTTAAT+TGG - chr7.4:22639444-22639463 MS.gene033735:intron 30.0%
TCATGGATGAATTATTCTCA+AGG - chr7.4:22640802-22640821 MS.gene033735:CDS 30.0%
TCGAAAAAATTGAACTACTG+TGG - chr7.4:22638543-22638562 MS.gene033735:intron 30.0%
TGTTTCACTTCCTTTATAGT+AGG + chr7.4:22640597-22640616 None:intergenic 30.0%
TTAGCTTAAGCTTAAACCAT+GGG - chr7.4:22638258-22638277 MS.gene033735:CDS 30.0%
TTATTGGATGATCTAGATAC+CGG - chr7.4:22640460-22640479 MS.gene033735:intron 30.0%
TTCTATAAAATTCCCCTGTA+GGG - chr7.4:22640522-22640541 MS.gene033735:CDS 30.0%
TTTCTATAAAATTCCCCTGT+AGG - chr7.4:22640521-22640540 MS.gene033735:CDS 30.0%
! ACAGGGGAATTTTATAGAAA+AGG + chr7.4:22640521-22640540 None:intergenic 30.0%
! ATTAGCTTAAGCTTAAACCA+TGG - chr7.4:22638257-22638276 MS.gene033735:CDS 30.0%
! GCTTTCTTGGTTGAATATTT+TGG + chr7.4:22639288-22639307 None:intergenic 30.0%
! TCTCTTTTAATAGCTGAGTT+TGG - chr7.4:22640771-22640790 MS.gene033735:CDS 30.0%
! TGAGTTTGGTGAAAATATCA+TGG - chr7.4:22640785-22640804 MS.gene033735:CDS 30.0%
! TGGTGAAATTACTTCTTTTG+AGG - chr7.4:22639479-22639498 MS.gene033735:CDS 30.0%
! TGTTGGAAAGTAGTTGATTT+AGG - chr7.4:22638173-22638192 MS.gene033735:CDS 30.0%
!!! ATCTTTTTTTGATGATGGTC+TGG - chr7.4:22638862-22638881 MS.gene033735:intron 30.0%
!!! ATTACTTCTTTTGAGGTACT+AGG - chr7.4:22639486-22639505 MS.gene033735:CDS 30.0%
!!! GAAGACTTTTAAAATCGGTT+GGG - chr7.4:22640656-22640675 MS.gene033735:CDS 30.0%
!!! TGAAGACTTTTAAAATCGGT+TGG - chr7.4:22640655-22640674 MS.gene033735:CDS 30.0%
AAAACAAGCTCTGTGATACT+TGG + chr7.4:22640748-22640767 None:intergenic 35.0%
AACTTACTTGTGATAGCCAA+TGG + chr7.4:22638490-22638509 None:intergenic 35.0%
ACAGTGCATAATACATGCAA+AGG + chr7.4:22638901-22638920 None:intergenic 35.0%
ATCAGGTAGTAACTTCAAGA+TGG + chr7.4:22638336-22638355 None:intergenic 35.0%
ATCCATTTCATCTTGGATTG+AGG - chr7.4:22640160-22640179 MS.gene033735:intron 35.0%
ATCCTCAATCCAAGATGAAA+TGG + chr7.4:22640165-22640184 None:intergenic 35.0%
ATTAGAAACTAGCGTCAAGA+CGG - chr7.4:22638649-22638668 MS.gene033735:intron 35.0%
ATTCTTCCTATTGTGTCCAT+TGG - chr7.4:22638471-22638490 MS.gene033735:intron 35.0%
ATTGAGTTTCCTCTTGAACA+TGG + chr7.4:22639203-22639222 None:intergenic 35.0%
CACATATTTCATACACGATG+TGG - chr7.4:22640345-22640364 MS.gene033735:intron 35.0%
CTCTTTCTTGTTGAATGCTT+TGG + chr7.4:22638364-22638383 None:intergenic 35.0%
GAAGTTTAGGTCATTTGTAG+GGG - chr7.4:22640083-22640102 MS.gene033735:intron 35.0%
GAGGTTTATGTTGAGCAATT+TGG - chr7.4:22639366-22639385 MS.gene033735:intron 35.0%
GATCAACAATATCTTCTTGC+AGG + chr7.4:22640689-22640708 None:intergenic 35.0%
GCATAATACATGCAAAGGTT+TGG + chr7.4:22638896-22638915 None:intergenic 35.0%
GGAAGTTTAGGTCATTTGTA+GGG - chr7.4:22640082-22640101 MS.gene033735:intron 35.0%
TAGATATTACCCTTGATGAG+TGG + chr7.4:22639315-22639334 None:intergenic 35.0%
TTCTTCAACTAAACCCTACA+GGG + chr7.4:22640538-22640557 None:intergenic 35.0%
TTGAGTTTCCTCTTGAACAT+GGG + chr7.4:22639202-22639221 None:intergenic 35.0%
TTTCTTGTTGAATGCTTTGG+TGG + chr7.4:22638361-22638380 None:intergenic 35.0%
! CAAAGCATTCAACAAGAAAG+AGG - chr7.4:22638362-22638381 MS.gene033735:CDS 35.0%
! GAATTTTCTACAGTCACGAT+CGG - chr7.4:22639401-22639420 MS.gene033735:intron 35.0%
! TTGAGGTAAATTTGGAATGC+TGG + chr7.4:22638287-22638306 None:intergenic 35.0%
!! GTTGATTTAGGTTGTTCTTC+AGG - chr7.4:22638185-22638204 MS.gene033735:CDS 35.0%
!!! CTAGTTTTTTTTCCCCACAA+AGG + chr7.4:22640257-22640276 None:intergenic 35.0%
AAAACCAAACGACGTGATCT+AGG + chr7.4:22639703-22639722 None:intergenic 40.0%
AAACAAGTGATCGAGGTAGA+AGG - chr7.4:22640612-22640631 MS.gene033735:CDS 40.0%
AAAGCAGAACCACTCATCAA+GGG - chr7.4:22639303-22639322 MS.gene033735:intron 40.0%
ACACATACAAGTGTAGAGGT+TGG - chr7.4:22639669-22639688 MS.gene033735:CDS 40.0%
ACATAAACCTCATACACCGA+TGG + chr7.4:22639357-22639376 None:intergenic 40.0%
ACATACAAGTGTAGAGGTTG+GGG - chr7.4:22639671-22639690 MS.gene033735:CDS 40.0%
ACTCACACATACAAGTGTAG+AGG - chr7.4:22639665-22639684 MS.gene033735:CDS 40.0%
CACATACAAGTGTAGAGGTT+GGG - chr7.4:22639670-22639689 MS.gene033735:CDS 40.0%
CTTCTTCAACTAAACCCTAC+AGG + chr7.4:22640539-22640558 None:intergenic 40.0%
GAGACAACCATAAGTGCATT+TGG + chr7.4:22638212-22638231 None:intergenic 40.0%
GGGAAGTTTAGGTCATTTGT+AGG - chr7.4:22640081-22640100 MS.gene033735:intron 40.0%
GTGGGGAAAAAAAACTAGCT+AGG - chr7.4:22640259-22640278 MS.gene033735:intron 40.0%
TCTCAATACACACAGAGAGA+GGG + chr7.4:22639008-22639027 None:intergenic 40.0%
TCTTCAACTAAACCCTACAG+GGG + chr7.4:22640537-22640556 None:intergenic 40.0%
TGAATGCTTTGGTGGAAATC+AGG + chr7.4:22638353-22638372 None:intergenic 40.0%
TGATAGCCAATGGACACAAT+AGG + chr7.4:22638480-22638499 None:intergenic 40.0%
TTCAGGACCAAATGCACTTA+TGG - chr7.4:22638202-22638221 MS.gene033735:CDS 40.0%
TTCTCAATACACACAGAGAG+AGG + chr7.4:22639009-22639028 None:intergenic 40.0%
TTTAGGTCATTTGTAGGGGT+AGG - chr7.4:22640087-22640106 MS.gene033735:intron 40.0%
!!! TTCTTTTGAGGTACTAGGCA+TGG - chr7.4:22639491-22639510 MS.gene033735:CDS 40.0%
AAACCTCATACACCGATGGA+GGG + chr7.4:22639353-22639372 None:intergenic 45.0%
AACAAGAATGAGCCCTCCAT+CGG - chr7.4:22639338-22639357 MS.gene033735:intron 45.0%
ACGATCGGAAGAACTAGCAT+TGG - chr7.4:22639416-22639435 MS.gene033735:intron 45.0%
CAAACCTAGATCACGTCGTT+TGG - chr7.4:22639696-22639715 MS.gene033735:CDS 45.0%
CAAGAAAGAGGAGAGAATCC+TGG - chr7.4:22638374-22638393 MS.gene033735:CDS 45.0%
CTCAATACACACAGAGAGAG+GGG + chr7.4:22639007-22639026 None:intergenic 45.0%
GAAAGCAGAACCACTCATCA+AGG - chr7.4:22639302-22639321 MS.gene033735:intron 45.0%
GCATGTATGAAGCATGTTCC+AGG + chr7.4:22638395-22638414 None:intergenic 45.0%
GCTTCATACATGCAACACCA+GGG - chr7.4:22638402-22638421 MS.gene033735:CDS 45.0%
GGAAGTGAAACAAGTGATCG+AGG - chr7.4:22640605-22640624 MS.gene033735:CDS 45.0%
TAAACCTCATACACCGATGG+AGG + chr7.4:22639354-22639373 None:intergenic 45.0%
TCAATACACACAGAGAGAGG+GGG + chr7.4:22639006-22639025 None:intergenic 45.0%
TGCTTCATACATGCAACACC+AGG - chr7.4:22638401-22638420 MS.gene033735:CDS 45.0%
TGTAGGGGTAGGAGTTCAAT+TGG - chr7.4:22640098-22640117 MS.gene033735:intron 45.0%
TTTCCTCTTGAACATGGGAG+TGG + chr7.4:22639197-22639216 None:intergenic 45.0%
! ACTAGCATTGGATGGTGTGA+TGG - chr7.4:22639428-22639447 MS.gene033735:intron 45.0%
! GATGAGTGGTTCTGCTTTCT+TGG + chr7.4:22639301-22639320 None:intergenic 45.0%
! TCGGAAGAACTAGCATTGGA+TGG - chr7.4:22639420-22639439 MS.gene033735:intron 45.0%
!! GTCGTTGATAGCAAGATGAG+AGG - chr7.4:22640711-22640730 MS.gene033735:CDS 45.0%
!! ATTATTGAAATAAAAAAATT+TGG - chr7.4:22638734-22638753 MS.gene033735:intron 5.0%
AATACACACAGAGAGAGGGG+GGG + chr7.4:22639004-22639023 None:intergenic 50.0%
ACTCAACGAGATGGTCCAAG+AGG - chr7.4:22639515-22639534 MS.gene033735:CDS 50.0%
AGGCATGGTACTCAACGAGA+TGG - chr7.4:22639506-22639525 MS.gene033735:CDS 50.0%
AGTCTTCCATAGAAGCTCCC+TGG + chr7.4:22638422-22638441 None:intergenic 50.0%
CAATACACACAGAGAGAGGG+GGG + chr7.4:22639005-22639024 None:intergenic 50.0%
TGACCACTCCCATGTTCAAG+AGG - chr7.4:22639191-22639210 MS.gene033735:intron 50.0%
! TTTGGAATGCTGGTAGCCCA+TGG + chr7.4:22638277-22638296 None:intergenic 50.0%
ATACACACAGAGAGAGGGGG+GGG + chr7.4:22639003-22639022 None:intergenic 55.0%
GCAACACCAGGGAGCTTCTA+TGG - chr7.4:22638413-22638432 MS.gene033735:CDS 55.0%
GAGCCCTCCATCGGTGTATG+AGG - chr7.4:22639347-22639366 MS.gene033735:intron 60.0%
TACACACAGAGAGAGGGGGG+GGG + chr7.4:22639002-22639021 None:intergenic 60.0%
ACACACAGAGAGAGGGGGGG+GGG + chr7.4:22639001-22639020 None:intergenic 65.0%
ACACAGAGAGAGGGGGGGGG+GGG + chr7.4:22638999-22639018 None:intergenic 70.0%
CACACAGAGAGAGGGGGGGG+GGG + chr7.4:22639000-22639019 None:intergenic 70.0%
ACAGAGAGAGGGGGGGGGGG+GGG + chr7.4:22638997-22639016 None:intergenic 75.0%
CACAGAGAGAGGGGGGGGGG+GGG + chr7.4:22638998-22639017 None:intergenic 75.0%
! AGAGAGAGGGGGGGGGGGGG+GGG + chr7.4:22638995-22639014 None:intergenic 80.0%
! CAGAGAGAGGGGGGGGGGGG+GGG + chr7.4:22638996-22639015 None:intergenic 80.0%
! AGAGAGGGGGGGGGGGGGGG+GGG + chr7.4:22638993-22639012 None:intergenic 85.0%
! GAGAGAGGGGGGGGGGGGGG+GGG + chr7.4:22638994-22639013 None:intergenic 85.0%
! AGAGGGGGGGGGGGGGGGGG+GGG + chr7.4:22638991-22639010 None:intergenic 90.0%
! GAGAGGGGGGGGGGGGGGGG+GGG + chr7.4:22638992-22639011 None:intergenic 90.0%
! AGGGGGGGGGGGGGGGGGGG+GGG + chr7.4:22638989-22639008 None:intergenic 95.0%
! GAGGGGGGGGGGGGGGGGGG+GGG + chr7.4:22638990-22639009 None:intergenic 95.0%
! GGGGGGGGGGGGGGGGGGGA+AGG + chr7.4:22638982-22639001 None:intergenic 95.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 22638107 22640908 22638107 ID=MS.gene033735
chr7.4 mRNA 22638107 22640908 22638107 ID=MS.gene033735.t1;Parent=MS.gene033735
chr7.4 exon 22640513 22640908 22640513 ID=MS.gene033735.t1.exon1;Parent=MS.gene033735.t1
chr7.4 CDS 22640513 22640908 22640513 ID=cds.MS.gene033735.t1;Parent=MS.gene033735.t1
chr7.4 exon 22639479 22639733 22639479 ID=MS.gene033735.t1.exon2;Parent=MS.gene033735.t1
chr7.4 CDS 22639479 22639733 22639479 ID=cds.MS.gene033735.t1;Parent=MS.gene033735.t1
chr7.4 exon 22638107 22638472 22638107 ID=MS.gene033735.t1.exon3;Parent=MS.gene033735.t1
chr7.4 CDS 22638107 22638472 22638107 ID=cds.MS.gene033735.t1;Parent=MS.gene033735.t1
Gene Sequence

>MS.gene033735

ATGAAGGTGAAGACTTTACTTGATGAGAATTTGAAAATGATGATATCGGACACAACTTTTAATAGTTGTTGGAAAGTAGTTGATTTAGGTTGTTCTTCAGGACCAAATGCACTTATGGTTGTCTCAAATATCATGAAAGTCATTGATAAAATTAGCTTAAGCTTAAACCATGGGCTACCAGCATTCCAAATTTACCTCAATGATCTATATGAAAATGATTTCAATACCATCTTGAAGTTACTACCTGATTTCCACCAAAGCATTCAACAAGAAAGAGGAGAGAATCCTGGAACATGCTTCATACATGCAACACCAGGGAGCTTCTATGGAAGACTGTTTCCCAATAATTACATAGATTTTTTTCATTCTTCCTATTGTGTCCATTGGCTATCACAAGTAAGTTGCTCCAAACCAAATAGTTTTAATTCATCAATTATCGAAAAAATTGAACTACTGTGGTAAATTAGAGACATAAATAATGATTTTTTTTTTCTAATTTTTCTATCCTGAATATTTTTTTTTTACCAACAAATAAGAATTTTATTAGAAACTAGCGTCAAGACGGAAATTGTCTTAAATTTAGTTAAATATGTCTAATTAATTTAGATATATTTAACTACTAAAATGATTATTGAAATAAAAAAATTTGGTGATCTTAAAAAAGGATAAGAAAATCTCTTTCGATAATATTGTTGTATTTACGGAGACAAATGTACTATAAAAAGGGATTTCCTTCTTTTTGTGTATTATTATTTATCTTTTTTTGATGATGGTCTGGTTTGAACCCCAAACCTTTGCATGTATTATGCACTGTTCCTATCAACTAAGTTAAGTTCATGAGAACCTATTATTATTTATCTATTTGATAGGTGCCTTCCCCCCCCCCCCCCCCCCCCCCCCCTCTCTCTGTGTGTATTGAGAATACCTTAATTATGTGTTCTCAATTTAATACAACATTTTAGCTGTTTTTAAAAAACATACACTACGTGTCAACGTTTAATTCGTAGTATTTTGCATTAAATTAAGTTTTTTTTTTTTGTAAATAGTCTATTTTACTAAGATTATTTAGACTCAGCATTATGAATGACCACTCCCATGTTCAAGAGGAAACTCAATCATTTAGGTGTCTTTACATATAGTTGATCTTGTTTTATTTGTTAATGGTGGTATATTAGGCACCAAAATATTCAACCAAGAAAGCAGAACCACTCATCAAGGGTAATATCTACATAACAAGAATGAGCCCTCCATCGGTGTATGAGGTTTATGTTGAGCAATTTGGAAGAGATTTCAAGAATTTTCTACAGTCACGATCGGAAGAACTAGCATTGGATGGTGTGATGGTACTAACTTTAATTGGGAGAGAAAATAATGGTGAAATTACTTCTTTTGAGGTACTAGGCATGGTACTCAACGAGATGGTCCAAGAGGTAAATATGATTGATTAATATATGTTTGATAATTAATCAGTATATGAAAGCTTAATTAAACACTTAAAAATAGTACACACTTATAAATAGTCGATTTTTTATAGACAAAATGACAAATTATCATTGAAACTCACACATACAAGTGTAGAGGTTGGGGTTCAAACCTAGATCACGTCGTTTGGTTTTACAATTTCAAAAAATTTATCAATTAAGTTAAGACTTGTGGAATGATAATACACGCACTTTATATCTCCTAATCGCTTCCTACAATATTTTTACTTTTGAAATTTAATAACGATAGAAGATATAATACAACTATATAACTTGACCCTAAAATCTTTCAAAAAAAAAAAAATACTTGACCCTAAAAAAAAACTATATAACTTGACATTTTATATCATATATAATTTTGTAAAAAAAATCATATATAAAGATATTAATTACTCCACTGGTAGTATAAAGTCATTTTACACTTGCATCCAATGAAAATATATGGTGATATAATGTCAATTGTTATGAAAATTGTTGAAGTCAAAAATAATTTGGGAAGTTTAGGTCATTTGTAGGGGTAGGAGTTCAATTGGTGAGTGTGAAGTATAATAATCATCTTTCGCTAATTTGATATCCATTTCATCTTGGATTGAGGATTTCTCCTCTTTCCTCTATCTTGTAATTGCAACTTATTATTTTCTGAATTCTTCTTTTTAATAAACTTTTCCTTTGTGGGGAAAAAAAACTAGCTAGGATGAATTATTCTAGTTTTTAAAATACTCTCAATTTTTTGTTGATAATTTTAATCGTTCTTTTACACATATTTCATACACGATGTGGTACATGTAGTCATGTTATTATTTTTACAATTATCCTTTTGCCAATTTGAATCTTTGTTAATATAGTATTTATGTTTTCTATTGATCATCATATTATTGGATGATCTAGATACCGGAATAAAAATTATGTTTTGTTTTAACTTATGCTTTGCCTTTTCTATAAAATTCCCCTGTAGGGTTTAGTTGAAGAAGCAAAATTGGACTTGTTTAATTTGCCTCTATATCATCCTACTATAAAGGAAGTGAAACAAGTGATCGAGGTAGAAGGATCTTTTATCATTCAAACATTGAAGACTTTTAAAATCGGTTGGGATGCAAACCTGCAAGAAGATATTGTTGATCATGTCGTTGATAGCAAGATGAGAGGAGAGTTCATAGCCAAGTATCACAGAGCTTGTTTTGAATCTCTTTTAATAGCTGAGTTTGGTGAAAATATCATGGATGAATTATTCTCAAGGTTTGCAAAGCTAATTGCACAATTCATTGAGATAAAGTCACCAGATTTTTTCAATATTGTGTTGTTTATGACCAAGTGTCCTTAA
Protein sequence

>MS.gene033735.t1

MKVKTLLDENLKMMISDTTFNSCWKVVDLGCSSGPNALMVVSNIMKVIDKISLSLNHGLPAFQIYLNDLYENDFNTILKLLPDFHQSIQQERGENPGTCFIHATPGSFYGRLFPNNYIDFFHSSYCVHWLSQAPKYSTKKAEPLIKGNIYITRMSPPSVYEVYVEQFGRDFKNFLQSRSEELALDGVMVLTLIGRENNGEITSFEVLGMVLNEMVQEGLVEEAKLDLFNLPLYHPTIKEVKQVIEVEGSFIIQTLKTFKIGWDANLQEDIVDHVVDSKMRGEFIAKYHRACFESLLIAEFGENIMDELFSRFAKLIAQFIEIKSPDFFNIVLFMTKCP